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All Journal Jurnal Ilmu Pertanian Indonesia Jurnal Sain Veteriner Buletin Peternakan Jurnal Peternakan Indonesia JITRO (Jurnal Ilmiah dan Teknologi Peternakan Tropis) Jurnal Kedokteran Hewan Annales Bogorienses
Kaiin, Ekayanti Mulyawati
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology Veterinary Other

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Biological Analysis of Leydig Cells-Conditioned Medium to Support Rat Bone Marrow Mesenchymal Stem Cells Differentiation Kaiin, Ekayanti Mulyawati; Prasetyaningtyas, Wahono Esthi; Mohamad, Kusdiantoro; Djuwita, Ita; Yusuf, Tuty Laswardi; Setiadi, Mohamad Agus
Annales Bogorienses Vol. 22 No. 1 (2018): Annales Bogorienses
Publisher : BRIN

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Abstract

The developed Leydig cells-conditioned medium (LCM) contains bioactive materials secreted by Leydig cells in vitro. LCM was used to evaluate the ability of bone marrow mesenchymal stem cells differentiation. Bone marrow mesenchymal stem cells (1x 106 cell/ml) were cultured in : 1) DMEM supplemented with 10% NBCS as a control (M), 2) M supplemented with 10 ng/ml testosterone; 3) M supplemented with 50% LCM ; 4) M supplemented with 50% LCM and 2.5 IU/ml hCG. Bone marrow mesenchymal stem cells that were cultured with LCM has a positive reaction (57.4%) to histochemistry staining 3β-HSD and produced 1.87 ng/ml testosterone. Supplementation of hCG to LCM increased the positive number of Leydig cells and testosterone production by 74.6% and 12.33 ng/ml (P<0.05). It can be concluded that Leydig cells-conditioned medium can support differentiation of bone marrow mesenchymal stem cells into Leydig cells.
Co-Authors ACHMAD FARAJALLAH Ananda, Ananda Bambang Purwantara Dyah Perwitasari Faisal Amri Satrio Ferdy Saputra Hariono Hariono Hasbi Hermansyah, Alfandy Herry Sonjaya ITA DJUWITA Kartiawati Alipin Kurnia, Asep Kusdiantoro Mohamad Kuswati Kuswati M Agus Setiadi Madihah Madihah, Madihah Maulana, Tulus Moch. Syamsul Arifin Zein, Moch. Syamsul Arifin Muhammad Gunawan Mutmainna, Mutmainna Nafisah, Ghina Ni Wayan Kurniani Karja Niken Ulupi Nurkarimah, Dona Astari Paskah Partogi Agung Qhatimah, Husnul Rudi Afnan Rusherdiannita, Raden Cindy Saputra, Wandi Sri Gustina Sudirman Baco, Sudirman Syahruddin Said Tri Eko Susilorini Tuti Suryati TUTY LASWARDI YUSUF Wahono Esthi Prasetyaningtyas