Article Per Year (5 Year)
p-Index From 2021 - 2026
1.607
P-Index
This Author published in this journals
All Journal Jurnal Ilmu Pertanian Indonesia Jurnal Sain Veteriner Buletin Peternakan Jurnal Peternakan Indonesia JITRO (Jurnal Ilmiah dan Teknologi Peternakan Tropis) Jurnal Kedokteran Hewan Jurnal Bioteknologi & Biosains Indonesia (JBBI) Annales Bogorienses Jurnal Bioteknologi & Biosains Indonesia
Kaiin, Ekayanti Mulyawati
Unknown Affiliation
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science Health Professions Immunology & microbiology Medicine & Pharmacology Veterinary Other

Published : 13 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 13 Documents
Search

 1   2 
Biological Analysis of Leydig Cells-Conditioned Medium to Support Rat Bone Marrow Mesenchymal Stem Cells Differentiation Kaiin, Ekayanti Mulyawati; Prasetyaningtyas, Wahono Esthi; Mohamad, Kusdiantoro; Djuwita, Ita; Yusuf, Tuty Laswardi; Setiadi, Mohamad Agus
Annales Bogorienses Vol. 22 No. 1 (2018): Annales Bogorienses
Publisher : BRIN

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

The developed Leydig cells-conditioned medium (LCM) contains bioactive materials secreted by Leydig cells in vitro. LCM was used to evaluate the ability of bone marrow mesenchymal stem cells differentiation. Bone marrow mesenchymal stem cells (1x 106 cell/ml) were cultured in : 1) DMEM supplemented with 10% NBCS as a control (M), 2) M supplemented with 10 ng/ml testosterone; 3) M supplemented with 50% LCM ; 4) M supplemented with 50% LCM and 2.5 IU/ml hCG. Bone marrow mesenchymal stem cells that were cultured with LCM has a positive reaction (57.4%) to histochemistry staining 3β-HSD and produced 1.87 ng/ml testosterone. Supplementation of hCG to LCM increased the positive number of Leydig cells and testosterone production by 74.6% and 12.33 ng/ml (P<0.05). It can be concluded that Leydig cells-conditioned medium can support differentiation of bone marrow mesenchymal stem cells into Leydig cells.
CHARACTERISTICS AND KINEMATICS OF RAM FROZEN-THAWED SPERM MOVEMENT WITH DIFFERENT LECITHIN TYPES Damanik, Asman Ramadhan; Karja, Ni Wayan Kurniani; Setiadi , Mohamad Agus; Kaiin, Ekayanti Mulyawati
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 12 No. 2 (2025)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2025.10407

Abstract

This study evaluated the effectiveness of animal, plant, and synthetic lecithin-based extenders in preserving local Indonesian ram semen during cryopreservation. Post-thaw sperm quality was assessed using Computer-Assisted Sperm Analysis (CASA), focusing on motility, viability, membrane integrity, and kinematics. Data were analyzed using ANOVA and Duncan’s Multiple Range Test (DMRT) at a 95% significance level. Results showed that sperm motility, viability, and membrane integrity were significantly higher (P<0.05) in the animal lecithin-based extender (53.66±1.33%, 66.45±2.50%, and 67.68±2.33%, respectively) compared to plant (51.84±0.48%, 62.98±1.94%, and 64.27±1.51%) and synthetic (50.14±0.22%, 60.72±0.81%, 62.44±1.37%) extenders. While kinematic parameters were not significantly different among groups, synthetic lecithin showed slightly higher values in velocity-related measures, whereas animal lecithin resulted in the most consistent improvement in overall sperm quality. All lecithin-based extenders met the Indonesian National Standard (SNI) for semen quality, and sperm kinematics remained within optimal fertilization values after cryopreservation.
CHARACTERISTICS AND KINEMATICS OF RAM FROZEN-THAWED SPERM MOVEMENT WITH DIFFERENT LECITHIN TYPES Damanik, Asman Ramadhan; Karja, Ni Wayan Kurniani; Setiadi , Mohamad Agus; Kaiin, Ekayanti Mulyawati
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 12 No. 2 (2025)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2025.10407

Abstract

This study evaluated the effectiveness of animal, plant, and synthetic lecithin-based extenders in preserving local Indonesian ram semen during cryopreservation. Post-thaw sperm quality was assessed using Computer-Assisted Sperm Analysis (CASA), focusing on motility, viability, membrane integrity, and kinematics. Data were analyzed using ANOVA and Duncan’s Multiple Range Test (DMRT) at a 95% significance level. Results showed that sperm motility, viability, and membrane integrity were significantly higher (P<0.05) in the animal lecithin-based extender (53.66±1.33%, 66.45±2.50%, and 67.68±2.33%, respectively) compared to plant (51.84±0.48%, 62.98±1.94%, and 64.27±1.51%) and synthetic (50.14±0.22%, 60.72±0.81%, 62.44±1.37%) extenders. While kinematic parameters were not significantly different among groups, synthetic lecithin showed slightly higher values in velocity-related measures, whereas animal lecithin resulted in the most consistent improvement in overall sperm quality. All lecithin-based extenders met the Indonesian National Standard (SNI) for semen quality, and sperm kinematics remained within optimal fertilization values after cryopreservation.
Co-Authors ACHMAD FARAJALLAH Ananda, Ananda Bambang Purwantara Damanik, Asman Ramadhan Dyah Perwitasari Faisal Amri Satrio Ferdy Saputra Hariono Hariono Hasbi Hermansyah, Alfandy Herry Sonjaya ITA DJUWITA Kartiawati Alipin Kurnia, Asep Kusdiantoro Mohamad Kuswati Kuswati M Agus Setiadi Madihah Madihah, Madihah Maulana, Tulus Moch. Syamsul Arifin Zein, Moch. Syamsul Arifin Muhammad Gunawan Mutmainna, Mutmainna Nafisah, Ghina Ni Wayan Kurniani Karja Niken Ulupi Nurkarimah, Dona Astari Paskah Partogi Agung Qhatimah, Husnul Rudi Afnan Rusherdiannita, Raden Cindy Saputra, Wandi Setiadi , Mohamad Agus Sri Gustina Sudirman Baco, Sudirman Syahruddin Said Tri Eko Susilorini Tuti Suryati TUTY LASWARDI YUSUF Wahono Esthi Prasetyaningtyas