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All Journal Jurnal Teknologi Laboratorium Jurnal Penelitian Kesehatan Suara Forikes Health Notions THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST Journal Of Vocational Health Studies Window of Health : Jurnal Kesehatan Amerta Nutrition Jurnal Ilmu dan Teknologi Kesehatan Jurnal Analis Medika Biosains (JAMBS) INDONESIAN JOURNAL OF MEDICAL LABORATORY SCIENCE AND TECHNOLOGY Poltekita : Jurnal Ilmu Kesehatan JPP Jurnal Kesehatan Poltekkes Palembang Jaringan Laboratorium Medis Medica Arteriana (Med-Art) Medicra (Journal of Medical Laboratory Science/Technology) CENDEKIA : Jurnal Ilmu Pengetahuan Devotion: Journal of Research and Community Service International Journal of Advanced Health Science and Technology HEALTHY: Jurnal Inovasi Riset Ilmu Kesehatan Jurnal Ilmu Multidisplin Analis Kesehatan Sains (e-Journal) Jurnal Rakat Sehat : Pengabdian Kepada Masyarakat Jurnal Pengabdian Kesehatan Beguai Jejama JPK : Jurnal Penelitian Kesehatan Frontiers in Community Service and Empowerment Jurnal Ilmu Kesehatan Strada Jurnal Ilmiah Kesehatan (SJIK) Folia Medica Indonesiana
Evy Diah Woelansari
Jurusan Teknologi Laboratorium Medis Poltekkes Kemenkes Surabaya
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Health Education for Elementary School Age Children in the Kenosis Community, Surabaya Museyaroh; Juliana Christyaningsih; Yuni Ginarsih; Kasiati; Mujayanto; Nuning Marina Pengge; Kiaonarni O.W; Teresia Retna P; Pratiwi Hermiyanti; Rahayu Sumaningsih; Lembunai Tat Alberta; Evi Yunita Nugrahini; Yohanes Kambaru W; Teta puji Rahayu; Nurwening TW; Hery Sumasto; Suliati; Lully Hani Endarini; Evy Diah Woelansari; Ira Puspita Sari; Ira Rahayu Tiyar
Frontiers in Community Service and Empowerment Vol. 5 No. 1 (2026): March
Publisher : Forum Ilmiah Teknologi dan Ilmu Kesehatan (FORITIKES)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35882/ficse.v4i2.96

Abstract

ABSTRACT The tutoring center "Kenosis" in Sidoyoso, established in 2016 by GKA Gloria Pos Sidoyoso, provides free educational support for elementary school children. This program helps in the academic and character development of children, initially taught by instructors from the Inter-University Christian Fellowship (Perkantas) and now reinforced by 4 permanent teachers. In addition to the need for tutoring, children are faced with daily conditions such as unhealthy snacks, low understanding of dental hygiene, and the importance of handwashing. These bad habits increase the risk of dental caries, infectious diseases, and the consumption of harmful food additives. Education and supervision are key to improving children's health and well-being. The Poltekkes Kemenkes Surabaya service team, consisting of 21 lecturers and 11 students, provided solutions to the above issues to 35 elementary school children at the Kenosis tutoring center on January 9, 2025, by delivering educational materials and practicing how to choose healthy snacks, brush teeth, and wash hands properly and correctly. Knowledge of harmful food additives The average pre-test score was 60.2, while the post-test score was 70.5. Understanding of proper tooth brushing techniques pre-test result 60.0 and post-test 70.5, understanding of good and correct handwashing habits had an average pre-test score of 60.0 and a post-test score of 70.5. There was an increase in knowledge about harmful food additives, proper tooth brushing techniques, and proper handwashing at the "Kenosis GKA Gloria Pos Sidoyoso" tutoring session. INDEX TERMS GKA Gloria, preservatives, food, PHBS, teeth.
Perbandingan Pemeriksaan Skrining Antibodi (Indirect Coombs Test) dengan Direct Coombs Test pada Pasien dengan Riwayat Transfusi Darah Berulang Shela Yuni Arta; Suhariyadi Suhariyadi; Evy Diah Woelansari; Christ Kartika Rahayuningsih
Jaringan Laboratorium Medis Vol 7 No 2 (2025): November 2025
Publisher : Poltekkes Kemenkes Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31983/jlm.v7i2.13643

Abstract

Repeated blood transfusions can increase risk of alloimmunization and autoimunization, which causes formation alloantibodies and autoantibodies. The presence of these antibodies can make cross-matching more difficult and increase the possibility of hemolytic transfusion risk. Antibody screening tests (Indirect Coombs Test) and Direct Coombs Test play a role in detecting the presence of alloantibodies and autoantibodies in patient with a history of repeated transfusion. The aim of the study was to determine the diferences in results between antibody screening (Indirect Coombs Test) and Direct Coombs Test in patients receiving repeated blood transfusions. This study used a quasi-experimental design with a cross-sectional approach. The subjects were patients with a history of blood transfusions more than 5 times at RSUD R.T. Notopuro Sidoarjo totalling 60 samples. The data obtained were then analyzed statistically to see if there were any significant differences between the two types of examinations. The results obtained showed a significant difference between the results of the antibody screening test (ICT) and the Direct Coombs Test (DCT) (p-value 0.000), that more patients with repeated blood transfusions had positive DCT results (75%). Some patients showed positive results on one or both types of tests, indicating the presence of alloantibodies or autoantibodies. ICT and DCT tests provide complementary information in detecting antibodies in patients with repeated transfusions. The use of both is important in improving the safety of blood transfusions and minimizing the risk of hemolytic reactions.
PENGARUH WAKTU PEMERIKSAAN GLUKOSA DARAH MENGGUNAKAN TABUNG PLAIN DAN TABUNG SERUM SEPARATOR Qonita Ardiyanti Maysarah; Anik Handayati; Museyaroh Museyaroh; Evy Diah Woelansari
HEALTHY : Jurnal Inovasi Riset Ilmu Kesehatan Vol. 5 No. 4 (2026)
Publisher : Pusat Pengembangan Pendidikan dan Penelitian Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51878/healthy.v5i4.14080

Abstract

Blood glucose testing is one of the most important laboratory examinations for the diagnosis, monitoring, and evaluation of diabetes mellitus management. The accuracy of test results is influenced by various factors, particularly those occurring during the pre-analytical phase, including the type of blood collection tube used and the delay between sample collection and analysis. Delayed examination may reduce blood glucose concentration due to ongoing glycolysis within blood cells, thereby affecting the validity of laboratory results. This study aimed to analyze the effect of delayed examination time on blood glucose levels using plain tubes and Serum Separator Tubes (SST). An experimental study with a Two-Way Repeated Measures design was conducted. Serum samples obtained from seven respondents were collected into plain tubes and SST tubes and analyzed at 0, 5, and 10 hours using a Biobase Automatic Chemistry Analyzer. Data were analyzed using the Shapiro–Wilk normality test and Two-Way Repeated Measures ANOVA. The results showed that the mean blood glucose level in plain tubes decreased from 75.57 mg/dL at 0 hours to 64.29 mg/dL at 5 hours and 65.43 mg/dL at 10 hours. In contrast, blood glucose levels in SST tubes remained relatively stable at 75.71 mg/dL, 76.86 mg/dL, and 78.14 mg/dL at 0, 5, and 10 hours, respectively. The data were normally distributed (p > 0.05). Two-Way Repeated Measures ANOVA demonstrated that examination time, tube type, and their interaction had a statistically significant effect on blood glucose levels (p < 0.05). These findings indicate that delayed examination affects blood glucose levels in plain tubes, whereas SST tubes are able to maintain blood glucose stability for up to 10 hours after sample collection. ABSTRAK Pemeriksaan kadar glukosa darah merupakan salah satu pemeriksaan laboratorium yang berperan penting dalam menegakkan diagnosis, memantau perkembangan penyakit, serta mengevaluasi keberhasilan terapi pada pasien diabetes mellitus. Keakuratan hasil pemeriksaan dipengaruhi oleh berbagai faktor, khususnya pada tahap pra-analitik, seperti jenis tabung yang digunakan dan lamanya waktu antara pengambilan sampel dengan proses pemeriksaan. Penundaan pemeriksaan dapat menyebabkan penurunan kadar glukosa akibat proses glikolisis yang masih berlangsung di dalam sel darah sehingga memengaruhi validitas hasil pemeriksaan. Penelitian ini bertujuan untuk menganalisis pengaruh penundaan waktu pemeriksaan terhadap kadar glukosa darah menggunakan tabung plain dan Serum Separator Tube (SST). Penelitian menggunakan desain eksperimen dengan pendekatan Two Way Repeated Measure. Sampel berupa serum dari tujuh responden yang dimasukkan ke dalam tabung plain dan SST, kemudian dianalisis pada waktu 0 jam, 5 jam, dan 10 jam menggunakan Biobase Automatic Chemistry Analyzer. Analisis data dilakukan menggunakan uji normalitas Shapiro-Wilk dan uji Two Way Repeated Measure ANOVA. Hasil penelitian menunjukkan bahwa rerata kadar glukosa pada tabung plain mengalami penurunan dari 75,57 mg/dL pada 0 jam menjadi 64,29 mg/dL pada 5 jam dan 65,43 mg/dL pada 10 jam. Sebaliknya, kadar glukosa pada tabung SST tetap relatif stabil, yaitu 75,71 mg/dL, 76,86 mg/dL, dan 78,14 mg/dL pada masing-masing waktu pengamatan. Data penelitian berdistribusi normal (p>0,05). Hasil analisis menunjukkan bahwa waktu pemeriksaan, jenis tabung, serta interaksi keduanya memberikan pengaruh yang bermakna terhadap kadar glukosa darah (p<0,05). Dengan demikian, penundaan pemeriksaan menyebabkan perubahan kadar glukosa pada tabung plain, sedangkan penggunaan tabung SST mampu mempertahankan stabilitas kadar glukosa darah hingga 10 jam setelah pengambilan sampel.
KORELASI HASIL UJI WIDAL DENGAN RAPID TEST IgG DAN IgM PADA PASIEN SUSPEK DEMAM TIFOID Fazira Azmi Haq; Evy Diah Woelansari; Christ Kartika Rahayuningsih; Wisnu Istanto
HEALTHY : Jurnal Inovasi Riset Ilmu Kesehatan Vol. 5 No. 4 (2026)
Publisher : Pusat Pengembangan Pendidikan dan Penelitian Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51878/healthy.v5i4.14121

Abstract

Typhoid fever is a systemic infectious disease caused by Salmonella enterica serovar Typhi and requires laboratory examinations to support diagnosis. The Widal test and IgG/IgM rapid test are serological examinations that are still widely used in healthcare facilities, but they have different test characteristics. This study aimed to analyze the correlation between Widal test results and IgG and IgM rapid test results in patients with suspected typhoid fever at Dr. H. Moh. Anwar Regional General Hospital, Sumenep. This study employed an analytical observational design with a cross-sectional approach and purposive sampling technique. The study was conducted from February 2 to 28, 2026, involving 35 patients with suspected typhoid fever. The examinations included the slide agglutination Widal test and immunochromatographic IgG/IgM rapid test. Data were analyzed using Spearman's correlation test with a significance level of p < 0.05. The results showed a significant correlation between the Widal test and the IgG rapid test, with a correlation coefficient of r = 0.667 and p < 0.001, indicating a strong positive correlation. A significant correlation was also found between the Widal test and the IgM rapid test, with r = 0.861 and p < 0.001, indicating a very strong positive correlation. In conclusion, there was a significant correlation between Widal test results and both IgG and IgM rapid test results in patients with suspected typhoid fever. These findings indicate an association between the results of the two serological examinations; however, their interpretation should consider the patient's clinical condition and the stage of disease progression. ABSTRAK Demam tifoid merupakan penyakit infeksi sistemik yang disebabkan oleh Salmonella enterica serovar Typhi dan memerlukan pemeriksaan penunjang untuk membantu diagnosis. Uji Widal dan rapid test IgG/IgM merupakan pemeriksaan serologis yang masih digunakan di fasilitas pelayanan kesehatan, tetapi memiliki karakteristik pemeriksaan yang berbeda. Penelitian ini bertujuan menganalisis korelasi hasil uji Widal dengan rapid test IgG dan IgM pada pasien suspek demam tifoid di RSUD Dr. H. Moh. Anwar Sumenep. Penelitian menggunakan desain observasional analitik dengan pendekatan cross-sectional dan teknik purposive sampling. Penelitian dilaksanakan pada 2–28 Februari 2026 dengan jumlah sampel sebanyak 35 pasien suspek demam tifoid. Pemeriksaan dilakukan menggunakan uji Widal metode slide aglutinasi dan rapid test IgG/IgM metode imunokromatografi. Data dianalisis menggunakan uji korelasi Spearman dengan tingkat kemaknaan p < 0,05. Hasil penelitian menunjukkan terdapat korelasi signifikan antara hasil uji Widal dengan rapid test IgG dengan koefisien korelasi r = 0,667 dan p < 0,001 yang menunjukkan hubungan kuat. Korelasi antara hasil uji Widal dengan rapid test IgM juga signifikan dengan r = 0,861 dan p < 0,001 yang menunjukkan hubungan sangat kuat. Dengan demikian, terdapat korelasi signifikan antara hasil uji Widal dengan rapid test IgG dan IgM pada pasien suspek demam tifoid. Hasil tersebut menunjukkan adanya keterkaitan antara hasil kedua pemeriksaan serologis, tetapi interpretasinya tetap perlu mempertimbangkan kondisi klinis dan fase perjalanan penyakit.
SCREENING PASIEN SUSPEK DEMAM TIFOID DENGAN WIDAL TES DAN RAPID TEST IgM ANTI-SALMONELLA DAN RASIO MONOSIT–LIMFOSIT (MLR) Marshanda Pratiwi Miftahurrahma; Evy Diah Woelansari; Museyaroh Museyaroh; Dwi Krihariyani
HEALTHY : Jurnal Inovasi Riset Ilmu Kesehatan Vol. 5 No. 4 (2026)
Publisher : Pusat Pengembangan Pendidikan dan Penelitian Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51878/healthy.v5i4.14375

Abstract

Typhoid fever is an infectious disease caused by Salmonella typhi and remains a major public health problem in Indonesia. The diagnosis of typhoid fever is generally confirmed through serological tests such as the Widal test and the anti-Salmonella IgM rapid test. Both tests are commonly used, but they have limitations in sensitivity and specificity. The monocyte-lymphocyte ratio (MLR) is a hematological parameter that has the potential to serve as a supportive indicator because it reflects both innate and adaptive immune responses. The objective of this study was to analyze the relationship between the monocyte-lymphocyte ratio (MLR) and the Widal test and anti-Salmonella IgM rapid test in patients with suspected typhoid fever. This study was conducted using an observational analytical method with a cross-sectional design. The study sample consisted of 29 patients with suspected typhoid fever at the Klinik Ultra Medica in Tulungagung, selected through purposive sampling. The results showed that MLR values fell into the low category in 79.3% of cases, moderate in 17.2%, and high in 3.4%. The anti-Salmonella IgM rapid test results showed 37.9% positive and 62.1% negative. The Widal test results were dominated by a titer of 1/80 (79.3%) and a titer of 1/160 (13.8%). Spearman's rank correlation test yielded r = 0.200 (p = 0.298) between MLR and anti-Salmonella IgM rapid test, and r = -0.133 (p = 0.490) between MLR and Widal test. In conclusion, there is no significant association between the monocyte-to-lymphocyte ratio (MLR) and the results of either the Widal test or the anti-Salmonella IgM rapid test in patients with suspected typhoid fever (p > 0.05). ABSTRAK Demam tifoid merupakan penyakit infeksi yang disebabkan oleh bakteri Salmonella typhi dan hingga saat ini masih menjadi masalah kesehatan masyarakat di Indonesia. Penegakan diagnosis demam tifoid umumnya dilakukan melalui pemeriksaan serologi seperti tes Widal dan rapid test IgM anti-Salmonella. Keduanya masih sering digunakan, namun memiliki keterbatasan sensitivitas dan spesifisitas. Rasio monosit-limfosit (Monocyte-Lymphocyte Ratio / MLR) merupakan parameter hematologi yang berpotensi menjadi indikator pendukung diagnosis demam tifoid karena mencerminkan respons imun bawaan dan adaptif. Penelitian ini bertujuan untuk menganalisis hubungan rasio monosit-limfosit (MLR) dengan tes Widal dan rapid test IgM anti-Salmonella pada pasien suspek demam tifoid. Penelitian ini menggunakan metode observasional analitik dengan desain cross-sectional. Sampel penelitian berjumlah 29 pasien suspek demam tifoid di Klinik Ultra Medica Tulungagung yang dipilih secara purposive sampling. Hasil penelitian menunjukkan nilai MLR berada pada kategori rendah 79,3%, sedang 17,2%, dan tinggi 3,4%. Hasil rapid test IgM anti-Salmonella menunjukkan 37,9% positif dan 62,1% negatif. Hasil tes Widal didominasi oleh titer 1/80 sebesar 79,3% dan titer 1/160 sebesar 13,8%. Hasil uji korelasi Spearman Rank antara MLR dengan rapid test IgM anti-Salmonella menghasilkan  ( ), sedangkan antara MLR dengan tes Widal menghasilkan  ( ). Dengan demikian, tidak terdapat hubungan yang signifikan antara rasio monosit-limfosit (MLR) dengan hasil tes Widal maupun rapid test IgM anti-Salmonella pada pasien suspek demam tifoid ( ).    
GAMBARAN PEMERIKSAAN RHEUMATOID FACTOR PADA LANSIA DI WILAYAH KERJA PUSKESMAS PUCANG SEWU SURABAYA Widya Kartika Sari; Evy Diah Woelansari; Museyaroh Museyaroh; Dwi Krihariyani
CENDEKIA: Jurnal Ilmu Pengetahuan Vol. 6 No. 4 (2026)
Publisher : Pusat Pengembangan Pendidikan dan Penelitian Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51878/cendekia.v6i4.14049

Abstract

Rheumatoid Factor (RF) is an autoantibody commonly used as a supporting laboratory test for the identification of Rheumatoid Arthritis (RA). Rheumatoid Arthritis is an autoimmune disease characterized by chronic inflammation of the joints and may occur among older adults. This study aimed to describe the results of Rheumatoid Factor examination among elderly individuals in the working area of Pucang Sewu Public Health Center, Surabaya. This study employed a descriptive design involving 34 elderly respondents selected using purposive sampling based on predetermined inclusion and exclusion criteria. Rheumatoid Factor examination was performed using the latex agglutination method. Samples with reactive results were subsequently examined using a semi-quantitative method to determine the Rheumatoid Factor titer. Data were analyzed descriptively and presented in frequency distribution tables based on Rheumatoid Factor results, sex, and age group. The results showed that 31 respondents (91.2%) had negative Rheumatoid Factor results, while 3 respondents (8.8%) had positive results. All respondents with positive results had a Rheumatoid Factor titer of 16 IU/mL. Based on sex, all positive results were found among male respondents. Based on age group, positive results were found among respondents aged 60–69 years and 80–89 years. It can be concluded that most elderly individuals in the working area of Pucang Sewu Public Health Center, Surabaya, had negative Rheumatoid Factor results, while a small proportion had positive results with low titers. ABSTRAK Rheumatoid Factor (RF) merupakan salah satu autoantibodi yang digunakan sebagai pemeriksaan penunjang dalam identifikasi Rheumatoid Arthritis (RA). Rheumatoid Arthritis merupakan penyakit autoimun yang ditandai dengan peradangan kronis pada sendi dan dapat ditemukan pada kelompok lanjut usia. Penelitian ini bertujuan untuk mengetahui gambaran hasil pemeriksaan Rheumatoid Factor pada lansia di wilayah kerja Puskesmas Pucang Sewu Surabaya. Penelitian ini menggunakan desain deskriptif dengan melibatkan 34 responden lansia yang dipilih menggunakan teknik purposive sampling berdasarkan kriteria inklusi dan eksklusi. Pemeriksaan Rheumatoid Factor dilakukan menggunakan metode aglutinasi lateks. Sampel dengan hasil reaktif selanjutnya diperiksa secara semikuantitatif untuk menentukan titer Rheumatoid Factor. Data dianalisis secara deskriptif dan disajikan dalam bentuk tabel distribusi frekuensi berdasarkan hasil pemeriksaan Rheumatoid Factor, jenis kelamin, dan kelompok usia. Hasil penelitian menunjukkan bahwa 31 responden (91,2%) memiliki hasil Rheumatoid Factor negatif, sedangkan 3 responden (8,8%) menunjukkan hasil positif. Seluruh responden dengan hasil positif memiliki titer Rheumatoid Factor sebesar 16 IU/mL. Berdasarkan jenis kelamin, seluruh hasil positif ditemukan pada responden laki-laki. Berdasarkan kelompok usia, hasil positif ditemukan pada kelompok usia 60–69 tahun dan 80–89 tahun. Dapat disimpulkan bahwa sebagian besar lansia di wilayah kerja Puskesmas Pucang Sewu Surabaya memiliki hasil Rheumatoid Factor negatif, sedangkan sebagian kecil menunjukkan hasil positif dengan titer rendah.
GAMBARAN HASIL PEMERIKSAAN HEPATITIS B SURFACE ANTIGEN DAN ANTIBODI HEPATITIS C VIRUS PADA IBU HAMIL Vela Navira Rezi; Evy Diah Woelansari; Ratno Tri Utomo
CENDEKIA: Jurnal Ilmu Pengetahuan Vol. 6 No. 4 (2026)
Publisher : Pusat Pengembangan Pendidikan dan Penelitian Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51878/cendekia.v6i4.14316

Abstract

Hepatitis B and Hepatitis C remain public health problems that may cause serious complications in pregnant women and fetuses and can also be transmitted vertically from mother to baby. Early detection through laboratory examination during pregnancy is essential as an effort to prevent transmission and provide optimal management. This study aimed to determine the results of Hepatitis B Surface Antigen (HBsAg) and Hepatitis C Virus Antibody (Anti-HCV) examinations among pregnant women at Manding Public Health Center, Sumenep Regency. The study sample consisted of 32 pregnant women in the first and second trimesters who underwent laboratory examinations at Manding Public Health Center. The samples were selected using a purposive sampling technique based on the inclusion and exclusion criteria. Primary data were obtained from blood plasma samples examined using the immunochromatographic method (rapid test) according to standard operating procedures. Data were analyzed using descriptive methods to describe the frequency distribution of reactive and non-reactive results for HBsAg and Anti-HCV testing. This study was conducted at the Laboratory of Manding Public Health Center, Sumenep Regency, from February 1–28, 2026. The results showed that among 32 respondents, all obtained non-reactive HBsAg results (100%) and all Anti-HCV results were also non-reactive (100%). ABSTRAK Hepatitis B dan Hepatitis C masih menjadi masalah kesehatan masyarakat yang berpotensi menyebabkan komplikasi serius pada ibu hamil maupun janin, serta dapat ditularkan secara vertikal dari ibu ke bayi. Deteksi dini melalui pemeriksaan laboratorium pada masa kehamilan sangat penting dilakukan sebagai upaya pencegahan penularan serta penanganan yang optimal. Penelitian bertujuan untuk mengetahui gambaran hasil pemeriksaan Hepatitis B Surface Antigen (HBsAg) dan Antibodi Hepatitis C Virus (Anti-HCV) pada ibu hamil di Puskesmas Manding, Kabupaten Sumenep. Sampel penelitian terdiri dari 32 ibu hamil trimester I dan II yang menjalani pemeriksaan laboratorium di Puskesmas Manding, pengambilan sampel dilakukan menggunakan teknik purposive sampling berdasarkan kriteria inklusi dan ekslusi. Data primer diperoleh dari plasma darah yang diperiksa menggunakan metode immunokromatografi (rapid test) sesuai standar operasional prosedur. Analisis data dilakukan menggunakan metode deskriptif untuk menggambarkan distribusi frekuensi hasil  reaktif dan non-reaktif pada pemeriksaan HBsAg dan Anti-HCV. Penelitian dilaksanakan di Laboratorium Puskesmas Manding Kabupaten Sumenep pada periode 1-28 Februari 2026. Hasil menunjukkan bahwa dari 32 responden, seluruhnya mendapatkan hasil HBsAg non-reaktif (100%) dan hasil Anti-HCV non-reaktif (100%).
Gambaran Pemeriksaan Screening Hepatitis B dan Hepatitis C pada Ibu Hamil dengan Metode Imunokromatografi di Puskesmas Sidoarjo Melly Nury Sabriyani; Evy Diah Woelansari; Suhariyadi; Wisnu Istanto
JIKES : Jurnal Ilmu Kesehatan Vol. 5 No. 1 (2026): Juli-Desember 2026
Publisher : Yayasan Pendidikan Tanggui Baimbaian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.71456/jik.v5i1.2552

Abstract

Hepatitis B dan Hepatitis C adalah penyakit infeksi yang dapat ditularkan dari ibu kepada bayi, terutama selama proses kehamilan dan persalinan. Pemeriksaan skrining pada ibu hamil penting dilakukan sebagai upaya deteksi dini dan pencegahan penularan. Penelitian ini bertujuan untuk memperoleh gambaran hasil pemeriksaan skrining Hepatitis B dan Hepatitis C pada ibu hamil menggunakan metode imunokromatografi di Puskesmas Sidoarjo. Penelitian ini menggunakan desain deskriptif dengan pendekatan cross-sectional. Sampel penelitian terdiri atas 30 ibu hamil trimester I, II, dan III yang menjalani pemeriksaan antenatal care (ANC) di Puskesmas Sidoarjo. Pemeriksaan dilakukan menggunakan rapid test HBsAg dan Anti-HCV dengan metode imunokromatografi. Hasil penelitian menunjukkan bahwa seluruh responden memperoleh hasil nonreaktif pada pemeriksaan Hepatitis B menggunakan rapid test HbsAg sebanyak 30 orang (100%) dan pemeriksaan Hepatitis C menggunakan rapid test Anti-HCV sebanyak 30 orang (100%). penelitian ini menunujukkan tidak ditemukan infeksi Hepatitis pada ibu hamil di Puskesmas Sidoarjo. Diharapkan peneliti lanjutan menggunakan metode pemeriksaan yang lebih akurat dan sensitif, seperti ELISA atau PCR.
PERBANDINGAN KADAR HIGH SENSITIVITY C-REACTIVE PROTEIN (hs-CRP) METODE Fluoroescence Immunoassay (FIA) ANTARA SAMPEL SERUM DAN PLASMA EDTA PADA PENDERITA DIABETES MELITUS Kanayu Aulia Masardi; Suhariyadi Suhariyadi; Wisnu Istanto; Evy Diah Woelansari
HEALTHY : Jurnal Inovasi Riset Ilmu Kesehatan Vol. 5 No. 4 (2026)
Publisher : Pusat Pengembangan Pendidikan dan Penelitian Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51878/healthy.v5i4.13729

Abstract

Diabetes mellitus is a chronic metabolic disorder associated with increased inflammatory activity and contributes to the development of cardiovascular complications. High-sensitivity C-reactive protein (hs-CRP) serves as an inflammatory marker to evaluate low-grade systemic inflammation. Accurate specimen selection is crucial for analytical validity. This study aims to compare hs-CRP levels measured by Fluorescence Immunoassay (FIA) between serum and EDTA plasma specimens in patients with diabetes mellitus. An observational comparative study with a cross-sectional design was conducted using 20 paired serum and EDTA plasma samples from diabetes mellitus patients at Haji General Hospital, East Java Province, in March 2026. hs-CRP levels were measured using the FIA method. The mean hs-CRP concentration in serum was  ( ) and in EDTA plasma was  ( ). The Wilcoxon Signed Rank Test yielded a  of 0.463 ( ), indicating no statistically significant difference between serum and EDTA plasma hs-CRP levels. In conclusion, serum and EDTA plasma demonstrate analytical equivalence for hs-CRP measurement using the FIA method, allowing EDTA plasma to serve as a valid alternative specimen in routine laboratory practice. ABSTRAK Diabetes melitus merupakan gangguan metabolisme kronis yang berkaitan dengan peningkatan aktivitas inflamasi dan berkontribusi terhadap perkembangan komplikasi kardiovaskular. High-sensitivity C-reactive protein (hs-CRP) digunakan sebagai penanda inflamasi untuk menganalisis proses peradangan kronis tingkat rendah (low-grade inflammation). Ketepatan pemilihan spesimen laboratorium sangat memengaruhi validitas analitik. Penelitian ini bertujuan untuk membandingkan kadar hs-CRP metode Fluorescence Immunoassay (FIA) antara spesimen serum dan plasma EDTA pada penderita diabetes melitus. Penelitian observasional komparatif dengan desain cross-sectional ini dilakukan terhadap 20 pasang spesimen serum dan plasma EDTA dari pasien diabetes melitus di RSUD Haji Provinsi Jawa Timur pada Maret 2026. Kadar hs-CRP diukur menggunakan metode FIA. Hasil penelitian menunjukkan rerata kadar hs-CRP serum sebesar  ( ) dan plasma EDTA sebesar  ( ). Hasil uji Wilcoxon Signed Rank Test menghasilkan nilai  ( ), yang menunjukkan tidak terdapat perbedaan yang bermakna secara statistik antara kadar hs-CRP pada spesimen serum dan plasma EDTA. Kesimpulannya, serum dan plasma EDTA memiliki kesepadanan analitik dalam pemeriksaan hs-CRP metode FIA, sehingga plasma EDTA dapat digunakan sebagai spesimen alternatif yang valid dalam kondisi darurat laboratorium.  
Co-Authors ., Pestariati A.A. Ketut Agung Cahyawan W Abror, Yogi Khoirul Adelia Gita Prasasti Alifiah, Salsabiil Rochman Aliya Ayu Rizqiyah Anggraini, Anita Dwi Anita Dwi Anggraini Aprilyadi, Nadi Arifin, Syamsul Diah Aryati Aryati Ayu Puspitasari Cheppy Juwita Ayuningrum Christiyaningsih, Juliana Devan Andhika Diah Titik Mutiarawati Dini Widowati Dwi Hidayah Septiana Dwi Rahayu Edy Haryanto Eka Safitri, Devayanti Endah Astuti, Sri Sulami Endarini, Lully Hanni Entuy Kurniawan Kurniawan Evi Yunita Nugrahini Fadia Rakhmalia Fazira Azmi Haq Firli Rohmah Primula Dewi Fortuna Aliza Dewi Handayati, Anik Hariyanto Hariyanto Hermiyanti, Pratiwi Ira Puspita Sari Ira Rahayu Tiyar Istanto, Wisnu istiqomah istiqomah Istiqomah Istiqomah Juliana Christyaningsih Kanaya Aulia Masardi Kanayu Aulia Masardi Kartika Rahayuningsih Kartika Rahayuningsih, Christ Kasiati, Klanting Khambali, Khambali Kiaonarni O.W Krihariyani, Dwi Kumahartati, Intan Lembunai Tat Alberta Liliek Soetjiatie Lully Hani Endarini Luthfi Rusyadi Mamik Mamik Mamik Mamik Marshanda Pratiwi Miftahurrahma Melly Nury Sabriyani Minarti Minarti Minarti Minarti Mohd Nazil Salleh Mujayanto Museyaroh, Museyaroh Musholli Himmatun Nabilah Mutiarawati, Diah Titik Nabilah, Musholli Himmatun Niswata, Navila Arfianti Novikasari, Caturizkyana Nuning Marina Pengge Nur Zerlinda Nurwening TW Prasasti, Adelia Gita Purnomowati, Anik Purwati Purwati Puspa Wardani Qonita Ardiyanti Maysarah Rahayu Sumaningsih Rahayu, Teta Puji Rahma Yuliana, Putri Ramadhany, Rieke Dwi Ratno Tri Utomo Resty Cahya Pertiwi Retno Sasongkowati Retno Sasongkowati Rieke Dwi Ramadhany Riyanto, Digdo Rizqiyah, Aliya Ayu Salsabiil Rochman Alifiah Sasongkowati, Retno Shela Yuni Arta Shella Nur Laily Sri Utami Suhariyadi Suhariyadi Suliati Suliati Suliati Suliati Sumasto, Hery Syafira, Nurah Teresia Retna P Triasworo Nawangwulan Vela Navira Rezi Widiastutik, Dyah Widya Kartika Sari Win Darmanto Wisnu Istanto Wisnu Istanto Yohanes Kambaru W Yuni Ginarsih