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All Journal AL KAUNIYAH Jurnal Kesehatan Andalas Mutiara Medika: Jurnal Kedokteran dan Kesehatan Majalah Kesehatan FKUB Medical Laboratory Technology Journal Jurnal Surya Medika PengabdianMu: Jurnal Ilmiah Pengabdian kepada Masyarakat Jurnal Kedokteran Universitas Palangka Raya Pengabdian Kampus: Jurnal Informasi Kegiatan Pengabdian Pada Masyarakat JKKI : Jurnal Kedokteran dan Kesehatan Indonesia Barigas: Jurnal Riset Mahasiswa
Permatasari, Silvani
Department Of Biology, Faculty Of Medicine, Universitas Palangka Raya, Palangka Raya
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Economics, Econometrics & Finance Environmental Science Health Professions Immunology & microbiology Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Medicine & Pharmacology Nursing Public Health Social Sciences

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Pengaruh Rebusan Akar Saluang Belum (Lavanga sarmentosa) Terhadap Jumlah Spermatid pada Gambaran Histologis Tubulus Seminiferus Mencit: Effectivity of Saluang Belum (Lavanga sarmentosa) on The Spermatids in Tubulus Seminiferus Histological of Mice Silvani Permatasari; Hairunnida Rahmatina; Ratna Widayati; I Gde Eka A
Jurnal Surya Medika (JSM) Vol. 9 No. 2 (2023): Jurnal Surya Medika (JSM)
Publisher : Institute for Research and Community Services Universitas Muhammadiyah Palangkaraya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33084/jsm.v9i2.4346

Abstract

The Dayak tribe of Kalimantan is known as the root of Saluang Belum (Lavanga sarmentosa) a traditional medicinal plant that has properties to increase stamina, and sexual arousal and is used as an alternative medicine to increase male fertility. L.sarmentosa is consumed by drinking the boiled water of the plant's roots. The aim of this study is to determine the effect infusion roots of Saluang Belum (L.sarmentosa) in increasing the spermatids as seen in the tubulus seminiferus histological of mice (Mus musculus). The root will be extracted by the infusion method with different doses for each group, namely 200 mg/KgBB, 400 mg/KgBB, and 600 mg/KgBB, and negative controls (aquades) which was carried out for 15 days to mice. On the day 16, the right testis was taken and histological preparations were made with hematoxylin-eosin staining and observed under a microscope at 400x magnification in five fields of view. Compounds contained in the infusion of L.sarmentosa are alkaloids, saponins, triterpenoids, flavonoids, phenolics, and steroids. The number of spermatids in the tubulus seminiferus histological of mice increased statistically significantly from the doses of 200 mg/KgBB, 400 mg/KgBB, and 600 mg/KBBg. Conclusion: Infusion of the roots L.sarmentosa can increase the number of spermatids seen in the tubulus seminiferus histological of mice.
Analisis Hasil Deteksi SARS-COV2 Menggunakan Rapid Tes Antigen di Laboratorium Mikrobiologi Klinik RSUD dr. Doris Sylvanus Palangkaraya: Analysis of SARS-COV2 Detection Using Antigen Rapid Tes in the Laboratory Clinical Microbiology RSUD dr. Doris Sylvanus Palangkaraya Silvani Permatasari; Florence Felicia; Ni Putu W.S.W; Misbah Misbah; Arnino A; Dede Z. A; Yanuardi Ikhsan; Rini Fitri R; Chandra Chandra; Febrianto E. P
Jurnal Surya Medika (JSM) Vol. 9 No. 2 (2023): Jurnal Surya Medika (JSM)
Publisher : Institute for Research and Community Services Universitas Muhammadiyah Palangkaraya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33084/jsm.v9i2.4417

Abstract

The antigen rapid diagnostic test is used for screening COVID-19 patients and is easy to do anywhere and saves time. The sensitivity of various brands of rapid antigen test kits can vary, so medical personnel need to pay close attention to them. Testing for the SARS-CoV2 antigen kit needs to be done diagnostically. This study aims to determine the specificity, sensitivity, and accuracy of the Haelgen antigen rapid test through diagnostic tests. The study design was cross-sectional, with a total sample of 60 people who took swabs from the nasopharynx and oropharynx. The sample is placed in the viral transport medium (VTM). Samples were examined by RT-PCR and, at the same time, examined using an antigen rapid test. The results of the analysis were carried out by calculating the sensitivity, specificity, positive predictive value (PPV), negative predictive value (NPV), and accuracy of the SARS-Cov2 antigen test compared to standard RT-PCR using a diagnostic test formula. The most common characteristics of COVID-19 patients were female and 20–29 years old (28.3%). The results of the Haelgen rapid antigen diagnostic test showed a sensitivity of 92.31%, a specificity of 100%, a positive predictive value of 100%, a negative predictive value of 87.5%, and an accuracy of 95%. The sensitivity, specificity, and accuracy of the Haelgen rapid antigen are suitable for testing for COVID-19.
Identifikasi Bakteri pada Alat-Alat Medis Bedah Minor di Ruang UGD Puskesmas Pahandut Kota Palangka Raya: Identification of Bacteria on Minor Surgical Medical Devices in the er of Pahandut Public Helath Center Palangka Raya Sherly Marselina; Natalia Sri Martani; Silvani Permatasari
Jurnal Surya Medika (JSM) Vol. 9 No. 2 (2023): Jurnal Surya Medika (JSM)
Publisher : Institute for Research and Community Services Universitas Muhammadiyah Palangkaraya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33084/jsm.v9i2.5673

Abstract

Background: HAIs are infections that are obtained from hospitals or other health care facilities, such as health centers. According to the Ministry of Health, in Indonesia it reaches 15.74%, much higher than in developed countries, which ranges from 4.8 to 15.5%. According to the Indonesian Ministry of Health, the average is 8.1%. At the Puskesmas, the Emergency Unit (ER) is the place most at risk for HAIs, for patients undergoing treatment using less sterile equipment, such as minor surgical equipment. Object: To determine the presence or absence of bacteria and to identify the types of bacteria in minor surgical medical devices in the ER Pahandut Public Health Center, Palangka Raya City. Methods: This study used a descriptive quantitative design method, to determine the presence or absence of bacteria in sterilized minor surgical medical devices. Results: The identification results found gram-positive bacteria in 3 samples of minor surgical instruments, namely 2 samples identified as Staphylococcus aureus and 1 sample identified as Streptococcus sp. Conclusion : There are bacterial contaminants in minor surgical medical devices used in the ER Pahandut Public Health Center, Palangka Raya City.
Uji Efektivitas Gel Hand Sanitizer Ekstrak Daun Sembung (Blumea balsamifera) Sebagai Antimikroba Silvani Permatasari; Dewi Klarita Furtuna; Astrid Teresa; Syarpin Syarpin; Widya Krestina
Al-Kauniyah: Jurnal Biologi Vol 16, No 1 (2023): AL-KAUNIYAH JURNAL BIOLOGI
Publisher : Department of Biology, Faculty of Science and Technology, Syarif Hidayatullah State Islami

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15408/kauniyah.v16i1.22831

Abstract

AbstrakHand sanitizer merupakan salah satu bahan antiseptik yang yang praktis agar tubuh terhindar dari penyakit yang disebabkan oleh mikroorganisme. Hand sanitizer berbasis alkohol digunakan berlebihan dan terus menerus dapat mengakibatkan iritasi pada kulit. Kandungan yang terdapat dalam bahan alami sebagai alternatif pengganti alkohol antara lain flavonoid dan terpenoid yang terdapat di daun sembung (Blumea balsamifera). Penelitian ini bertujuan untuk mengetahui efektivitas gel hand sanitizer dari ekstrak daun sembung pada berbagai konsentrasi. Serbuk daun sembung diekstraksi secara maserasi menggunakan etanol 70%, kemudian dibuat gel dengan konsentrasi 10%, 15%, dan 20% ditambah carbopol 940, TEA, serta metil paraben. Sediaan gel hand sanitizer dievaluasi melalui uji organoleptis, pH, dan homogenitas selama 4 minggu. Setelah itu, dilakukan pengujian daya antiseptik dan aktivitas antimikroba terhadap Staphylococcus aureus, Escherichia coli, dan Candida albicans. Sediaan gel hand sanitizer ekstrak daun sembung memiliki konsistensi kental, warna hijau kehitaman, aroma khas daun sembung, homogenitas baik, dan nilai pH 6. Sediaan ini juga memiliki daya antiseptik yang baik mampu mengurangi jumlah koloni hingga 95% sama dengan sediaan gel berbasis etanol. Sediaan gel konsentrasi 20% memiliki zona hambat yang sangat kuat terhadap S. aureus dan C. albicans, serta kuat melawan E. coli. Gel hand sanitizer ekstrak Blumea balsamifera menunjukkan aktivitas antimikroba dan dapat digunakan menjadi kandidat agen antimikroba organik alami.Kata kunci: Antimikroba; Antiseptik; Ekstrak Blumea balsamifera; Hand sanitizer  AbstractHand sanitizer is one of antiseptic so that our body avoids diseases caused by microorganism. Hand sanitizer based on ethanol using continuously can cause skin irritation. The secunder metabolit contained in herbal as an alternative to hand sanitizer based ethanol contained flavonoid and terpenoid, are found in sembung leaves (Blumea balsamifera). The aim of this study is to determine the effectiveness of hand sanitizer gel from sembung leaf extract at various concentrations. Sembung leaf was extracted by maceration using 70% ethanol, and then gel was made with concentrations of 10%, 15%, and 20% plus carbopol 940, TEA, methyl paraben. The hand sanitizer gel preparation was evaluated through organoleptic, pH, and homogeneity tests for 4 weeks. After that, antiseptic activity and antimicrobial activity were tested against Staphylococcus aureus, Escherichia coli, and Candida albicans. The hand sanitizer gel preparation of sembung leaf extract has a thick consistency, blackish green color, a distinctive aroma of sembung leaves, good homogeneity, and a pH value of 6. The gel with a concentration of 20% had a very strong zone of inhibition against S. aureus and C. albicans, also strong against E. coli. Blumea balsamifera extract hand sanitizer gel exhibits antimicrobial activity and can be used as a candidate natural organic antimicrobial agent.
Photochemical and bioactivity examination of fractionated saluang belum root extract (Lavanga sarmentosa) on in-vitro human sperm motility Silvani Permatasari; Syarpin; Dwi Ari Pujianto
JKKI : Jurnal Kedokteran dan Kesehatan Indonesia JKKI, Vol 14, No 2, (2023)
Publisher : Faculty of Medicine, Universitas Islam Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20885/JKKI.Vol14.Iss2.art3

Abstract

Background: Saluang belum (Lavanga sarmentosa) is one of Kalimantan's typical plants, which is as efficacious as a traditional medicine to increase sexual activity and male fertility. Based on previous studies, the content of flavonoid and steroid in 70% ethanol extract of L. sarmentosa were able to affect sperm quality of mice. Studies related to L. sarmentosa are still limited for phytochemical test and their bioactivity on human spermatozoa motility in vitro.Objective: This study is to perform phytochemical tests of compound content in fractionation with eluents of high and low polarity, namely methanol and chloroform, and then to test their bioactivity on the motility of human spermatozoa in vitro.Methods: L. sarmentosa was extracted with 96% ethanol and fractionated by using a vacuum chromatography column with chloroform and methanol as the eluent. Then obtained samples were analysed by a quantitative phytochemical test. The samples used in-vitro human spermatozoa were divided into eleven groups: control group, group administered with L. sarmentosa extract eluent chloroform of 10, 50, 100, 500, and 1000 ng/mL, and same concentration with extract eluent methanol. Furthermore, the sperm motility was analysed by using a Computer Assisted Sperm Analyser (CASA). Results: The methanol and chloroform fraction of L. sarmentosa root extract contained metabolites, namely terpenoids, flavonoids, steroids, alkaloids, saponins, and tannins. The sperm motility increased significantly at the treatment group of the methanol and chloroform fractions compared to the control group. There was a significant difference between the sperm motility incubated with methanol and that with chloroform fraction at concentration 500 and 1000 ng/mL.Conclusion: The results of sperm motility were higher in the methanol fraction than those in the chloroform fraction.
Bioactivity Examination of Uncaria gambir (W.Hunter) Roxb on In Vitro Human Sperm Motility Silvani Permatasari; Nida Halisa; Agnes Frethernety
Medical Laboratory Technology Journal Vol. 9 No. 2 (2023): December
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31964/mltj.v9i2.563

Abstract

Globally, 48 million couples experience infertility, where male infertility factors contribute to 50% of cases. Spermatozoa motility is a crucial parameter in assessing male fertility. Antioxidants act as the body's defence against excessive ROS and can be used as a treatment for male infertility. One of the local plants in Central Kalimantan that is potentially rich in antioxidants is Bajakah Kalalawit (Uncaria gambir (W.Hunter) Roxb. However, there is limited research on the bioactivity of Bajakah Kalalawit on spermatozoa in vitro. This study aims to determine the effect of ethanol extract of Uncaria gambir (W. Hunter) Roxb on spermatozoa motility in vitro. Bajakah Kalalawit stems were extracted using a 3x24-hour maceration technique with 96% ethanol. After obtaining the concentrated extract, the secondary metabolite compound content was analyzed through a phytochemical screening. The sample used was in vitro human spermatozoa that were washed and added to Bigger Whitten Whittingham medium, then incubated at 37°C for 1 hour with a 96% ethanol extract of Bajakah Kalalawit at doses of 50ng/ml, 100ng/ml, 500ng/ml, and 1000ng/ml, as well as a control group (Bigger, Whitten & Whittingham medium only). The results showed that the compounds contained in the ethanol extract of Uncaria gambir (W.Hunter) Roxb were terpenoids, flavonoids, phenolics, steroids, saponins, alkaloids, and tannins. Spermatozoa motility significantly increased in the treatment groups starting from doses of 50ng/ml, 100ng/ml, 500ng/ml, and 1000ng/ml compared to the control group. Ethanol extract of Uncaria gambir (W.Hunter) Roxb could increase spermatozoa motility in vitro and succeeded in improving reproductive technology.
Literature review : hubungan aktivitas fisik dengan kejadian hipertensi Dhaniang, Natasya; Kahanjak, Donna Novina; Permatasari, Silvani
Barigas: Jurnal Riset Mahasiswa Vol. 1 No. 2 (2023): Barigas: Jurnal Riset Mahasiswa
Publisher : Fakultas Kedokteran Universitas Palangka Raya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37304/barigas.v1i2.7924

Abstract

Hypertension, also known as high blood pressure, is a worldwide health issue. Hypertension is a condition in which the systolic and diastolic blood pressures rise above the usual limit. Lack of physical activity as a risk factor for hypertension needs more attention because this factor is a factor that can be modified with minimal effort and cost.  Understanding the tendency of the relationship between physical activity and the incidence of hypertension. The literature review uses a systematic review approach, data sources, data sources from original articles in the Pubmed.gov and Google Scholar databases with the keywords relationship, physical activity, and hypertension. There were 21 journals that met the inclusion criteria and data quality analysis. Synthesis of data using the SPIDER method. Based on the results of a literature review it was found that in the twenty-four journals analyzed, there were 18 (86%) journals that stated they were related and 3 (14%) stated otherwise. Individuals with lower physical activity tend to have a higher risk of hypertension and the factors that influence it include diet, age, nutritional status, family history of hypertension, smoking to routine health checks.
The Effect of Methanol Fractıonated of Luvunga sarmentosa on In Vıtro Sperm Membrane Integrıty Permatasari, Silvani; Nuha, Alya Firyal; Ratnasari, Arini; Widayati, Ratna; Ka Praja, Rian
Medical Laboratory Technology Journal Vol. 10 No. 1 (2024): June
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31964/mltj.v10i1.571

Abstract

Infertility cases in the world are increasing in 2020, with 186 million individuals affected worldwide. The most common cause of male infertility is due to decreased sperm quality. Sperm membrane integrity is known to play an essential role in the fertilization ability of spermatozoa. The methanol fraction of Luvunga sarmentosa root extract has the highest antioxidant activity compared to other fractions and can improve sperm motility in vitro. This study aims to determine the effect of the methanol fraction of Luvunga sarmentosa root extract on human sperm membrane integrity and the effective concentration of Luvunga sarmentosa methanol extract fraction to improve sperm membrane integrity in vitro. The samples used were washed human spermatozoa in vitro, then added BWW medium, and incubated at 37℃ for 1 hour with methanol fraction of Luvunga sarmentosa root extract at concentrations of 100, 500, 1000, and 5000ng/ml as well as the control group (BWW only). Furthermore, 100uL of each sample was added to 1mL of Hypo-osmotic swelling (HOS) solution, then incubated at 37oC for 30 minutes to see the integrity of the spermatozoa membrane from the tail swelling. The results showed that sperm membrane integrity significantly increased at 100, 500, 1000, and 5000ng/mL concentrations compared to the control group. The methanol fraction of Luvunga sarmentosa root extract was most effective at a concentration of 100ng/mL. Methanol fraction of Luvunga sarmentosa root extract can improve human sperm membrane integrity.
The Correlation between Protein, Iron, and Vitamin C Intake with Hemoglobin Levels in Pregnant Women Sitorus, Elisabeth Prihana Rotua; Handayani, Septi; Balyas, Abi Bakring; Widayati, Ratna; Fatmaria, Fatmaria; Permatasari, Silvani
Mutiara Medika: Jurnal Kedokteran dan Kesehatan Vol 24, No 1 (2024): January
Publisher : Universitas Muhammadiyah Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18196/mmjkk.v24i1.17311

Abstract

Hemoglobin is an important component of the human body. It aims to transport oxygen and carbon dioxide. Anemia during pregnancy often occurs in the second trimester. Pregnant women tend to be vulnerable to malnutrition since their nutritional needs will increase to satisfy the needs of both the mother and the fetus. Protein intake plays a crucial role in the transportation of iron in the body. Meanwhile, vitamin C serves as a promoter of iron absorption. This research aims to determine and analyze the correlation between protein, iron, and vitamin C intake and hemoglobin levels in pregnant women at Kayon Public Health Center, Jekan Raya District. This research used an analytic observational method with a cross-sectional approach and a purposive sampling technique. Bivariate analysis was also applied by using the Spearman test. The latter results obtain a p-value of 0.748 between protein intake and hemoglobin level; a p-value of 0.222 between vitamin C intake and hemoglobin level; and a p-value of 0.050 between iron intake and hemoglobin level. There was a correlation between iron intake and hemoglobin level, yet there was no correlation between protein and vitamin C intake with hemoglobin level.
Aktivitas Antioksidan Ekstrak Etanol Bajakah Kalalawit (Uncaria gambir (W.Hunter) Roxb) Menggunakan Metode DPPH: Antioxidant Activity of Ethanol Extract of Bajakah Kalalawit (Uncaria gambir (W.Hunter) Roxb) Using DPPH Method Nida Halisa; Agnes Frethernety; Silvani Permatasari; Francisca Diana Alexandra; Ysrafil Ysrafil
Jurnal Surya Medika (JSM) Vol. 10 No. 2 (2024): Jurnal Surya Medika (JSM)
Publisher : Institute for Research and Community Services Universitas Muhammadiyah Palangkaraya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33084/jsm.v10i2.6151

Abstract

Antioksidan dalam mendetoksifikasi radikal bebas akan ikut teroksidasi sehingga untuk mencukupi kebutuhan tubuh diperlukan antioksidan eksogen. Antioksidan eksogen dapat diperoleh dari tanaman herbal salah satunya Bajakah Kalalawit. Bajakah Kalalawit (Uncaria gambir (W.Hunter) Roxb.) adalah salah satu jenis bajakah yang terdapat di Kelurahan Marang, Kalimantan Tengah yang berpotensi sebagai antioksidan. Penelitian ini bertujuan untuk menganalisis aktivitas antioksidan dan kekuatan antioksidan Bajakah Kalalawit (Uncaria gambir (W. Hunter) Roxb.). Batang Bajakah Kalalawit diekstrak dengan metode maserasi 3x24 jam menggunakan pelarut etanol 96%. Setelah didapatkan ekstrak kental kemudian dianalisis kandungan senyawa metabolit sekunder dengan uji fitokimia. Uji aktivitas antioksidan dibuat dengan konsentrasi ekstrak 20ppm, 40ppm, 60ppm, 80ppm, dan 100ppm dengan metode DPPH (2,2-diphenyl-1-picrylhydrazyl). Hasil penelitian menunjukkan senyawa yang terdapat dalam ekstrak etanol Bajakah Kalalawit (Uncaria gambir (W.Hunter) Roxb) adalah terpenoid, flavonoid, fenolik, steroid, saponin, alkaloid, dan tanin. Ekstrak etanol Bajakah Kalalawit memiliki aktivitas antioksidan yang sangat kuat dengan nilai IC50 8,69ppm.
Co-Authors Agnes Frethernety Agnes Frethernety Andreyan Cesarianto Anna Marthea Veronica Aprilliana, Riska Arini Ratnasari Arnino A Astri Widiarti ASTRID TERESA Astrid Teresa Balyas, Abi Bakring Chandra Chandra Dea Tutut Dede Z. A Dewi Klarita Furtuna Dewi Klarita Furtuna Dhaniang, Natasya Dwi Ari Pujianto Elsa Trinovita Ervi Audina Munthe Farah Fauziyah Fadhiyatulqalbi Ahmad Fatmaria, Fatmaria Febrianto E. P Florence Felicia Florence Felicia Francisca Diana Alexandra Fraulein Aryati Fraulein Aryati Furtuna, Dwi Klarita Galih Indra Permana Hairunnida Rahmatina Handayani, Septi Herlina Eka Shinta I Gde Eka A Idsan, Rakha Satya Indah Noniika Indria Augustina Jhon Paris Pasaribu Kahanjak, Donna Novina Krestina, Widya Lia Sasmithae Malasinta Malasinta Malinda, Okta Marliah, Dinda Masaris Mery Irayanti Misbah Misbah Muhammad Aldri Muntir Guru Singa Nabila Putri Aditya Natalia Resoldes LBN Tobing Natalia Sri Martani Nawan Nawan Ni Putu Saraswati Ni Putu W.S.W Nida Halisa Nida Halisa Nuha, Alya Firyal Nurfianto, Sigit Patricia, Tisha Rahmidiati Ramadhani, Ahmad Ratna Widayati Ratna Widayati Ria Eriani Rian Ka Praja Rini Fitri R Seiya, Saint Septi Handayani Septi Handayani Sherly Marselina Sitorus, Elisabeth Prihana Rotua Syamsul Arifin Syarpin Syarpin Syarpin Tangdibali, Annisa Zaskia Alexandra Tasya Tasya Theressia Angel Lady Saragih Tisha Patricia Toemon, Agnes Immanuela Tri Widodo Vani Vrenika Victoria Berliani Widayati, Ratna Widya Krestina Yanuardi Ikhsan Yenisa Jestin Ganindyatama Ysrafil Ysrafil Zoelva Oktaviannur