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All Journal Jurnal Akuakultur Indonesia Jurnal Ilmu Tanah dan Lingkungan (Journal of Soil Science and Environment) Jurnal Ilmu Lingkungan Jurnal Lahan Suboptimal Sains Tanah Biota IJFAC (Indonesian Journal of Fundamental and Applied Chemistry) Science and Technology Indonesia Jurnal Penelitian Sains IJEMS (Indonesian Journal of Environmental Management and Sustainability) Molekul: Jurnal Ilmiah Kimia Journal of Tropical Marine Science Berkala Penelitian Hayati SRIWIJAYA JOURNAL OF ENVIRONMENT BIOVALENTIA: Biological Research Journal Jurnal Medika: Medika
Hary Widjajanti
Jurusan Biologi FMIPA, Universitas Sriwijaya, Sumatera Selatan, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Decision Sciences, Operations Research & Management Dentistry Earth & Planetary Sciences Energy Environmental Science Health Professions Immunology & microbiology Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Nursing Physics Public Health

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Eukaryote microbes potential for bioflocs in the swamp aquaculture Marini Wijayanti; Tanbiyaskur; Jubaedah, Dade; Saputra, Ade Bayu; Genti, Karta Sari; Agustina; Nabila Saraswati; Siti Yuliani; Hary Widjajanti
Jurnal Akuakultur Indonesia Vol. 19 No. 1 (2020): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (493.034 KB) | DOI: 10.19027/jai.19.1.19-29

Abstract

ABSTRACT Eucaryote microbes have an important role in forming bioflocs in the brackishwater aquaculture ponds. Bioflocs become potential live feed for milkfish or crustacea. This study aimed to identify the potential of eukaryotic microbes in the brackishwater aquaculture as the biofloc candidates potential development. This study was done through the water quality assessment and potential indigenous microbes isolation approach. Sampling was retrieved from the water subcomposite and sediment on each intertidal inlet and outlet. The water quality of brackishwater pond and intertidal swamp tended to lack of nutrient as containing inoptimal dissolved oxygen, salinity, pH, and ammonia content for the milkfish culture. Therefore, liming and fertilizing should be done to enrich the indigenous microbes. Eucaryote microbe isolated from the enrichment media was the aquatic fungi (multicellular and unicellular fungi) and microalgae (bacillariophyta, chlorophyta, and cyanophyta). Fungi and yeast formed a floculation with microalgae. This form will become a biofloc candidate as a live feed and water quality controller for the development of brackishwater aquaculture in the swamp area. Keywords: eucaryote microbes, biofloc, aquaculture, pond, brackish water swamp ABSTRAK Mikrob eukariot berperan penting dalam pembentukan bioflok di tambak budidaya pada lahan rawa payau. Bioflok menjadi pakan alami untuk budidaya ikan bandeng atau udang. Tujuan riset ini adalah mengidentifikasi mikrob eukariot rawa payau potensial untuk pengembangan kandidat bioflok di akuakultur rawa payau. Riset ini dilakukan dengan pendekatan kualitas air serta isolasi mikrob indigenous tambak dan rawa pasang surut untuk mendapatkan isolat mikrob potensial sebagai kandidat pembentuk bioflok. Sampling dilakukan secara subkomposit pada air dan sedimen di setiap inlet dan outlet pasang surut. Kualitas air tambak dan rawa pasang surut menunjukkan kondisi oksigen terlarut, salinitas, pH, dan amonia berada di luar batas optimum budidaya ikan bandeng, sehingga perlu dilakukan pengapuran dan pemupukan untuk memperbanyak mikrob indigenous-nya. Isolasi mikrob eukariot dari media pengayaan diperoleh jenis fungi akuatik yang multiseluler dan uniseluler, sedangkan mikroalga yang diperoleh yaitu dari golongan Bacillariophyta, Chlorophyta, dan Cyanophyta. Fungi yang dapat membentuk hifa dapat merangkai mikroalga dan khamir untuk membentuk flok. Susunan ini diharapkan dapat menjadi bahan bioflok yang berguna sebagai pengendali kualitas air sekaligus pakan alami bagi pengembangan budidaya di lahan rawa payau. Kata kunci: mikrob eukariot, bioflok, budidaya, tambak, rawa payau
Antidiabetic Activity of Endophytic Fungi Extract from Leaves of Kembang bulan (Tithonia diversifolia (Hemsley) A. Gray) Through α-Amylase Enzyme Inhibition Putri, Hanindita Alifia; Elisa Nurnawati; Widjajanti, Hary
Science and Technology Indonesia Vol. 10 No. 2 (2025): April
Publisher : Research Center of Inorganic Materials and Coordination Complexes, FMIPA Universitas Sriwijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26554/sti.2025.10.2.350-359

Abstract

Diabetes mellitus is a serious health problem with an increasing number of sufferers. In this context, an important method for controlling blood sugar levels is the derivation of α-amylase enzyme inhibitors from natural materials such as endophytic fungi. Therefore, this research aimed to analyze the potential of endophytic fungi from Tithonia diversifolia leaves as α-amylase enzyme inhibitors, secondary metabolites, as well as the character and identity of potential endophytic fungi. The stages included isolation and identification of endophytic fungi, extraction of secondary metabolites, α-amylase inhibition tests, analysis of bioactive compounds using Thin Layer Chromatography (TLC), and identification of potential endophytic fungi. The results showed that DT3J1 (67.83 µg/mL), DM3J1 (69.36 µg/mL), DT2J2 (80.42 µg/mL), and DT4J1 (88.64 µg/mL) of the eight endophytic fungi isolates reported high potential as inhibitors of the α-amylase enzyme, with IC50 values ​classified as strong. Potential fungal extracts contain bioactive compounds such as alkaloids, flavonoids, phenols, and terpenoids. Meanwhile, molecular identification showed that potential endophytic fungi consisted of Curvularia pseudointermedia, Diaporthe passifloricola, Nodulisporium verrucosum, and Muyocopron laterale species. This research also provided scientific evidence on the potential of the endophytic fungus Tithonia diversifolia as a candidate for antidiabetic drugs.
Pengaruh Metode Filtrasi Terhadap Peningkatan Kualitas Fisik Air Muara Sungsang, Banyuasin Meiyerani, Jeni; Melki, Melki; Widjajanti, Hary; Rozirwan, Rozirwan; Aryawati, Riris
Journal of Tropical Marine Science Vol 8 No 1 (2025): Journal of Tropical Marine Science (on going)
Publisher : Universitas Bangka Belitung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33019/jour.trop.mar.sci.v8i1.5680

Abstract

Water quality in the form of turbidity, colour, odour, taste and suspended residue are significant issues in Sungsang Banyuasin. The filter utilizes a filtration method to remove solid particles and contaminants. The research site consists of Sungsang, Sungsang II, and Sungsang III areas. The purpose of this study was to determine the water quality in the form of colour, taste, odour, turbidity, suspended residue, and salinity, as well as the ability of the filter to overcome water quality. Filtration consists of sedimentation, filter membranes, and filter media consisting of zeolite, silica, anthracite, ferrolite, activated carbon, and bioring. Water quality measurements used organoleptic to determine odour and taste, handrefractometer to measure salinity, direct reading to measure suspended residue, and spectrophotometry to measure colour and turbidity. The breech water was odourless and tasteless, and the suspended residue value ranged from 17-19 mg/L below the quality standard of 50 mg/L. Colour and turbidity values are greater than 50 on their respective scales and salinity values range from 11-13 ppt. The filtration process successfully reduced the values of suspended residue, colour, turbidity, and salinity.
Pelatihan Pemanfaatan Bioteknologi untuk Produk Sabun Alami Ramah Lingkungan Masyarakat Hanum, Laila; Widjajanti, Hary; Nurnawati, Elisa; Kamal, Mustafa; Harvianti, Yuniar; Oktariansyah, Yadi
Jurnal Medika: Medika Vol. 4 No. 4 (2025)
Publisher : LPPM Universitas Pahlawan Tuanku Tambusai

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31004/8qyxsp08

Abstract

Pemanfaatan tanaman obat keluarga menjadi alternatif penting untuk meningkatkan kesehatan masyarakat secara alami dan berkelanjutan. Meski banyak tanaman seperti jeruk, manggis, rambutan, dan lidah buaya tersedia di sekitar rumah, pengetahuan masyarakat tentang penggunaannya sebagai bahan antiseptik masih terbatas. Padahal, bahan alami tersebut dapat diolah menjadi produk ramah lingkungan seperti sabun herbal, yang lebih aman dibandingkan sabun berbahan kimia yang berpotensi menimbulkan dampak negatif pada kulit dan lingkungan. Program pengabdian ini memperkenalkan bioteknologi sederhana melalui pemanfaatan ekstrak tanaman obat sebagai bahan pembuatan sabun antiseptik herbal. Kegiatan difokuskan pada edukasi dan pelatihan bagi ibu-ibu dan remaja putri di Desa Tanjung Atap Barat, Kecamatan Tanjung Batu, Kabupaten Ogan Ilir, desa binaan Universitas Sriwijaya. Metode pelaksanaan meliputi observasi, pelatihan, pendampingan, dan evaluasi. Program ini meningkatkan pengetahuan dan keterampilan masyarakat dalam memanfaatkan sumber daya lokal untuk mendukung pola hidup bersih dan sehat, sekaligus membuka peluang usaha berbasis produk herbal serta mendukung upaya pelestarian lingkungan.
Exploration of Plastic Degrading Bacteria in the Musi River, PalembangCity Widjajanti, Hary; Annisa Kemala Tasya; Laila Hanum
Biota Vol 11 No 2 (2025): Jurna Biota 2025
Publisher : Faculty of Science and Technology Universitas Islam Negeri Raden Fatah Palembang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19109/biota.v11i2.27347

Abstract

Musi River is a waterway in South Sumatra that faces increasing plastic pollution, threatening aquatic ecosystems and human life. This study aims to identify and evaluate plastic-degrading bacteria from Musi River using phenotypic and molecular approaches. The methods of this study include bacterial isolation, plastic degradation test, and Scanning Electron Microscopy (SEM) analysis were carried out to assess degradation efficiency and changes in surface morphology, phenotypic and molecular identification. The results showed that isolates S1I3, S1I5, and S2I1 had high plastic degradation potential, with levels of 38.03%, 34.73%, and 30.46%, respectively. SEM observations showed changes in surface morphology, including pores and cracks. Molecular identification confirmed that S1I3 was Bacillus proteolyticus (99.87%), while S1I5 and S2I1 matched Bacillus cereus (100%).
Co-Authors A. Wulandari A. Wulandari Abdul Madjid Agung Bimantara Agustina Alfarado, Daniel Alfia Rahma Kurniawati Ali Djamhuri Andika Puspita Dewi Angga Prasetya Mulya Annisa Kemala Tasya Aria Yanka Paula Putri Arum Setiawan Arum Setiawan Asiandu, Angga Puja Bambang Yudono Budiono Budiono Dade Jubaedah Dedi Rohendi Dedik Budianta E. Patriono E. Patriono Eka Amelia Elfita Elfita Elfita Elfita Elfita Elfita Elisa Nurnawati Ervina Mukharomah, Ervina Fitrya Fitrya Genti, Karta Sari Gusti Ayu Widayanti Handayani, Harminuke Eka Harminuke Eka Handayani Harvianti, Yuniar Hary Wahyudi Heni Yohandini Laila Hanum Maraulina, Hotly Mardiyanto Mardiyanto Mardiyanto Maria Lusia Marini Wijayanti Maulida Harwati Meiyerani, Jeni Melki Melki Muharni Muharni Munawar Munawar Munawar Munawar Muslim Muslim Mustafa Kamal Nabila Saraswati Neni Marlina Ni Luh Putu SR Nina Tanzerina Nita Aminasih Noviyanto NUNI GOFAR Nur Arifah Nurkayah, Nurkayah Oktiansyah, Rian Permana, Muhammad Aditya Putri, Amanda Rahmaniah Putri, Aria Yanka Paula Putri, Hanindita Alifia Rahmawati Ridwan Riris Aryawati Rosmania, Rosmania Rozirwan . Salni Salni Salni Saputra, Ade Bayu Sarno . Sarno Sarno Singgih Tri Wardana Siti Alharzsa Siti Yuliani Sri Pertiwi E Sutandar, Vivi Hendra Syarifah Tanbiyaskur Ummi Hiras Habisukan, Ummi Hiras Wuriesyliane Wuriesyliane Yadi Oktariansyah Yustina Hapida, Yustina