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All Journal Jurnal Akuakultur Indonesia Jurnal Ilmu Tanah dan Lingkungan (Journal of Soil Science and Environment) Jurnal Ilmu Lingkungan Jurnal Lahan Suboptimal Biota IJFAC (Indonesian Journal of Fundamental and Applied Chemistry) Science and Technology Indonesia Jurnal Penelitian Sains IJEMS (Indonesian Journal of Environmental Management and Sustainability) Molekul: Jurnal Ilmiah Kimia Journal of Tropical Marine Science Berkala Penelitian Hayati SRIWIJAYA JOURNAL OF ENVIRONMENT BIOVALENTIA: Biological Research Journal
Hary Widjajanti
Jurusan Biologi FMIPA, Universitas Sriwijaya, Sumatera Selatan, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Decision Sciences, Operations Research & Management Earth & Planetary Sciences Energy Environmental Science Immunology & microbiology Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Physics Public Health

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Antidiabetic Activity of Endophytic Fungi Extract from Leaves of Kembang bulan (Tithonia diversifolia (Hemsley) A. Gray) Through α-Amylase Enzyme Inhibition Putri, Hanindita Alifia; Elisa Nurnawati; Widjajanti, Hary
Science and Technology Indonesia Vol. 10 No. 2 (2025): April
Publisher : Research Center of Inorganic Materials and Coordination Complexes, FMIPA Universitas Sriwijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26554/sti.2025.10.2.350-359

Abstract

Diabetes mellitus is a serious health problem with an increasing number of sufferers. In this context, an important method for controlling blood sugar levels is the derivation of α-amylase enzyme inhibitors from natural materials such as endophytic fungi. Therefore, this research aimed to analyze the potential of endophytic fungi from Tithonia diversifolia leaves as α-amylase enzyme inhibitors, secondary metabolites, as well as the character and identity of potential endophytic fungi. The stages included isolation and identification of endophytic fungi, extraction of secondary metabolites, α-amylase inhibition tests, analysis of bioactive compounds using Thin Layer Chromatography (TLC), and identification of potential endophytic fungi. The results showed that DT3J1 (67.83 µg/mL), DM3J1 (69.36 µg/mL), DT2J2 (80.42 µg/mL), and DT4J1 (88.64 µg/mL) of the eight endophytic fungi isolates reported high potential as inhibitors of the α-amylase enzyme, with IC50 values ​classified as strong. Potential fungal extracts contain bioactive compounds such as alkaloids, flavonoids, phenols, and terpenoids. Meanwhile, molecular identification showed that potential endophytic fungi consisted of Curvularia pseudointermedia, Diaporthe passifloricola, Nodulisporium verrucosum, and Muyocopron laterale species. This research also provided scientific evidence on the potential of the endophytic fungus Tithonia diversifolia as a candidate for antidiabetic drugs.
Exploration of Plastic Degrading Bacteria in the Musi River, PalembangCity Widjajanti, Hary; Annisa Kemala Tasya; Laila Hanum
Biota Vol 11 No 2 (2025): Jurna Biota 2025
Publisher : Faculty of Science and Technology Universitas Islam Negeri Raden Fatah Palembang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19109/biota.v11i2.27347

Abstract

Musi River is a waterway in South Sumatra that faces increasing plastic pollution, threatening aquatic ecosystems and human life. This study aims to identify and evaluate plastic-degrading bacteria from Musi River using phenotypic and molecular approaches. The methods of this study include bacterial isolation, plastic degradation test, and Scanning Electron Microscopy (SEM) analysis were carried out to assess degradation efficiency and changes in surface morphology, phenotypic and molecular identification. The results showed that isolates S1I3, S1I5, and S2I1 had high plastic degradation potential, with levels of 38.03%, 34.73%, and 30.46%, respectively. SEM observations showed changes in surface morphology, including pores and cracks. Molecular identification confirmed that S1I3 was Bacillus proteolyticus (99.87%), while S1I5 and S2I1 matched Bacillus cereus (100%).
Pertumbuhan dan Hasil Padi pada Inseptisol Asal Rawa Lebak yang Diinokulasi Berbagai Konsorsium Bakteri Penyumbang Unsur Hara Wuriesyliane Wuriesyliane; Nuni Gofar; Abdul Madjid; Hary Widjajanti; Ni Luh Putu SR
Jurnal Lahan Suboptimal : Journal of Suboptimal Lands Vol. 2 No. 1 (2013)
Publisher : Research Center for Suboptimal Lands (PUR-PLSO), Universitas Sriwijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33230/JLSO.2.1.2013.32

Abstract

The aim of this research was to study the effect of multisynergism bacterial isolates growth of rice plants as well as to obtain best composition of endophytic bacterial isolates, Azotobacter, Azospirilium and phosphate solubilizing bacteria in spurring growth of rice plants. This research was conducted in October 2012 to February 2013. This study arranged in a completely randomized design (All treatments of multiple bacterial isolates resulted in increase of soil N x P Content). The results showed that multibacterial isolates B (endophytic bacteria + Azospirillum) increased vegetative and generative growth of rice plants. Treatment G (composition of endophytic bacterial isolates, Azotobacter, Azospirilium and phosphate solubilizing bacteria) can increase rice yields. Some soil N and P content increased in response to all treatments of multiple bacterial isolates. The similar result was also observed in the N and P uptake that some multiple bacterial isolates improved N and P uptake of plants
Co-Authors A. Wulandari A. Wulandari Abdul Madjid Agung Bimantara Agustina Alfarado, Daniel Alfia Rahma Kurniawati Ali Djamhuri Andika Puspita Dewi Angga Prasetya Mulya Annisa Kemala Tasya Aria Yanka Paula Putri Arum Setiawan Arum Setiawan Asiandu, Angga Puja Bambang Yudono Budiono Budiono Dade Jubaedah Dedi Rohendi Dedik Budianta E. Patriono E. Patriono Eka Amelia Elfita Elfita Elfita Elfita Elfita Elfita Elisa Nurnawati Ervina Mukharomah Fitrya Fitrya Genti, Karta Sari Gusti Ayu Widayanti Handayani, Harminuke Eka Harminuke Eka Handayani Hary Wahyudi Heni Yohandini Laila Hanum Maraulina, Hotly Mardiyanto Mardiyanto Mardiyanto Maria Lusia Marini Wijayanti Maulida Harwati Meiyerani, Jeni Melki Melki Muharni Muharni Munawar Munawar Muslim Muslim Nabila Saraswati Neni Marlina Ni Luh Putu SR Nina Tanzerina Nita Aminasih Noviyanto NUNI GOFAR Nur Arifah Nurkayah, Nurkayah Oktiansyah, Rian Permana, Muhammad Aditya Putri, Amanda Rahmaniah Putri, Aria Yanka Paula Putri, Hanindita Alifia Rahmawati Ridwan Riris Aryawati Rosmania, Rosmania Rozirwan . Salni Salni Salni Saputra, Ade Bayu Sarno . Sarno Sarno Singgih Tri Wardana Siti Alharzsa Siti Yuliani Sri Pertiwi E Sutandar, Vivi Hendra Syarifah Tanbiyaskur Ummi Hiras Habisukan, Ummi Hiras Wuriesyliane Wuriesyliane Yustina Hapida, Yustina