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All Journal Jurnal Akuakultur Indonesia Jurnal Ilmu Tanah dan Lingkungan (Journal of Soil Science and Environment) Jurnal Ilmu Lingkungan Jurnal Lahan Suboptimal Sains Tanah IJFAC (Indonesian Journal of Fundamental and Applied Chemistry) Science and Technology Indonesia Jurnal Penelitian Sains IJEMS (Indonesian Journal of Environmental Management and Sustainability) Molekul: Jurnal Ilmiah Kimia Journal of Tropical Marine Science Berkala Penelitian Hayati SRIWIJAYA JOURNAL OF ENVIRONMENT BIOVALENTIA: Biological Research Journal

Hary Widjajanti

Jurusan Biologi FMIPA, Universitas Sriwijaya, Sumatera Selatan, Indonesia

Author-ID : 201755

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Decision Sciences, Operations Research & Management Earth & Planetary Sciences Energy Environmental Science Immunology & microbiology Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Physics Public Health

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Pertumbuhan dan Hasil Padi pada Inseptisol Asal Rawa Lebak yang Diinokulasi Berbagai Konsorsium Bakteri Penyumbang Unsur Hara Wuriesyliane Wuriesyliane; Nuni Gofar; Abdul Madjid; Hary Widjajanti; Ni Luh Putu SR
Jurnal Lahan Suboptimal : Journal of Suboptimal Lands Vol. 2 No. 1 (2013)
Publisher : Research Center for Sub-optimal Lands (PUR-PLSO), Universitas Sriwijaya

The aim of this research was to study the effect of multisynergism bacterial isolates growth of rice plants as well as to obtain best composition of endophytic bacterial isolates, Azotobacter, Azospirilium and phosphate solubilizing bacteria in spurring growth of rice plants. This research was conducted in October 2012 to February 2013. This study arranged in a completely randomized design (All treatments of multiple bacterial isolates resulted in increase of soil N x P Content). The results showed that multibacterial isolates B (endophytic bacteria + Azospirillum) increased vegetative and generative growth of rice plants. Treatment G (composition of endophytic bacterial isolates, Azotobacter, Azospirilium and phosphate solubilizing bacteria) can increase rice yields. Some soil N and P content increased in response to all treatments of multiple bacterial isolates. The similar result was also observed in the N and P uptake that some multiple bacterial isolates improved N and P uptake of plants

IDENTIFIKASI DAN SINERGISME KAPANG LIPOLITIK DARI LIMBAH SBE (SPENT BLEACHING EARTH) SEBAGAI AGEN BIOREMEDIASI Ervina Mukharomah; Munawar Munawar; Hary Widjajanti
Jurnal Ilmu Lingkungan Vol 13, No 1 (2015): April 2015
Publisher : School of Postgraduate Studies, Diponegoro Univer

Identifikasi dan sinergisme lipolitik dari Limbah SBE (Spent Bleaching Earth) yang berpotensi sebagai agen bioremediasi telah dilakukan pada bulan Januari-Maret 2015. Pengambilan sampel dengan metode Random Sampling dari PT Wilmar International Group Mariana Sumatera Selatan. Penelitian dilakukan di Labolatorium Mikrobiologi Jurusan Biologi Fakultas Matematika dan Ilmu Pengetahuan Universitas Sriwijaya. Penelitian ini bertujuan untuk memperoleh isolat kapang lipolitik yang berpotensi mendegradasi residu minyak dan sebagai agen remediasi. Hasil penelitian ini diperoleh kapang lipolitik yang mampu mendegradasi minyak yaitu Cylindrocladium sp (V2), Fumago sp (V5) dan Aspergilus Fumigatus (V8). Ketiga isolat kapang ini dapat menurunkan residu minyak sebesar 76,6 %. Kapang yang memiliki potensi tinggi dalam mendegradasi minyak yaitu Aspergilus Fumigatus (V8).

Triacylglycerols produced by biomass of endophytic fungus Neopestalotiopsis surinamensis from the Scurrula atropurpurea Elfita Elfita; Muharni Muharni; Mardiyanto Mardiyanto; Fitrya Fitrya; Elisa Nurmawati; Hary Widjajanti
IJFAC (Indonesian Journal of Fundamental and Applied Chemistry) Vol 5, No 3 (2020): October 2020
Publisher : IJFAC (Indonesian Journal of Fundamental and Applied Chemistry)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24845/ijfac.v5.i3.95

In Indonesia, Scurrula atropurpurea is a medicinal plant known as benalu. Triacylglycerols can be produced by plants, animals, algae, and microorganisms such as endophytic fungi. Triacylglycerols can be used in cosmetics, food, and medicine because they have biological activities such as antitumor, antibacterial, and cytotoxic. Besides, the compound can be used as a biodiesel substitute for triacylglycerols sourced from oil palm. This study aims to isolate and characterize triacylglycerol from biomass of endophytic fungal N. surinamensis from the S. atropurpurea leaves. The compound was isolated and purified by the column chromatography method. The structure of the compound was determined by spectroscopic data ( FTIR, 1H-NMR, and 13C-NMR). Analysis of the spectrum and compared with the literature, the isolated compound is a triacylglycerol.

Bioremediation of Spent Bleaching Earth (SBE) Wastes using Lipolitic Bacteria (Bacillus cereus) with Variation of Inoculum Volume Maria Lusia; Nuni Gofar; Hary Widjajanti
Science and Technology Indonesia Vol. 3 No. 1 (2018): January
Publisher : Research Center of Inorganic Materials and Coordination Complexes, FMIPA Universitas Sriwijaya

Spent bleaching earth (SBE) is a solid waste was generated from the CPO refining step into cooking oil. SBE that was discharged directly into the environment has the potential to pollute the environment, because in the SBE waste contained oil and acid residues, which are easily to be oxidized and flammable. Therefore, SBE must be processed first before being discharged into the environment. One way to manage SBE is by bioremediation. Bioremediation is a method on cleaning the environment from contaminants by using biological agents, such as bacteria, fungi etc. The bacterial isolates used in this study were Bacillus cereus. This study aims to obtain the best inoculum and to know the ability of Bacillus cereus bacteria in degrading the oil content in SBE waste. This study used Completely Randomized Design with the volume of Bacillus cereus bacteria inoculum as a treatment, consisting of 6 treatment levels of 0 mL kg-1, 25 mL kg-1, 50 mL kg-1, 75 mL kg-1, 100 mL kg-1, 125 mL kg-1. Each treatment level was repeated 3 times, so taht 18 experimental units were obtained. Observation was done once a week, in a month. Parameters observed were bacterial population, percentage of oil degradation, and oil content degradation. The best treatment result for the bacterial population was obtained at the treatment of 100 mL kg-1, at week 4 which was 7,4 x 108 cfu g-1, and for the oil degradation was obtained at 50 mL kg-1 on the treatment at week 4 as big as 90,43%.

Phylogenetic Analysis Of Endophytic Fungi Isolate from Bellucia pentamera Naudin Based On ITS rDNA Andika Puspita Dewi; Elisa Nurnawati; Laila Hanum; Hary Widjajanti
Indonesian Journal of Environmental Management and Sustainability Vol. 3 No. 4 (2019): December
Publisher : Research Centre of Inorganic Materials and Complexs

Endophytic fungi can produce secondary metabolites of endophytic fungi isolated from host plants. The purpose of this study is to identify and analyze the sequencing of endophytic fungi isolates with ITS and Beta-tubulin markers and phylogenetic trees. The endophytic fungi isolate DKJ1, DKJ3a, DKJ3c and DKJ4 were successfully isolated from the cardia plant (Bellucia pentamera Naudin) indicated by Aspergillus niger group, Aspergillus fumigatus group and Penicillium sp. The results of the sequencing analysis of isolates DKJ1, DKJ3a, DKJ3c, and DKJ4 were successfully amplified with an annealing temperature of 54ºC with a pair of ITS1-ITS4 primers with a molecular weight of 570 bp and a Beta-Tubulin primer with an annealing temperature of 56.1ºC molecular weight of 550 bp. From the results of identification and analysis of DNA sequencing of endophytic fungi DKJ1, DKJ3a, DKJ3c and DKJ4 with the primary pair of ITS and Beta-tubulin shows that the phylogenetic tree is different from the species obtained. ITS DKJ1 isolates have similarities with the species Aspergillus piperis CBS 112811, ITS DKJ3c has similarities with the species Aspergillus flavus var flavus strain ATCC 16833, ITS DKJ3a has similarities with Penicillium rolfsii strain NRRL 1078 species and ITS DKJ4 has similarities with Penicillium oxalicum NRRL 787 species. Whereas isolate from DKJ1 Beta-Tubulin has similarities with NRRL 4875 Aspergillus tubingensis species, DKJ3c has similarities with species of Aspergillus novoparasiticus strain DTO 223-C4 and DKJ4 has a similarity with Penicillium guaibinensis species. But there are similarities based on Cluster A (Aspergillus Group) and Cluster B (Penicillium Group) on phylogenetic trees.

Antibacterial Activity of The Secondary Metabolites Produced by Endophytic Fungi Isolated from Acanthus ilicifolius Plant Gusti Ayu Widayanti; Hary Widjajanti; Salni Salni
Indonesian Journal of Environmental Management and Sustainability Vol. 3 No. 4 (2019): December
Publisher : Research Centre of Inorganic Materials and Complexs

Twelve endophytic fungi were isolated from the Acanthus ilicifolius plant. Two of them have the best antibacterial activity, namely Culvularia lunata and Diaporthe phaseolorum. Fungi can produce secondary metabolites that have the potential as antibacterial compounds. This study aims to isolate antibacterial compounds using column chromatography methods and test the antibacterial activity using diffusion methods to use paper discs. Isolation of secondary metabolites produces 27 eluates. From TLC, it is known that the endophytic fungi Culvularia lunata produce an active compound as an antibacterial that is phenol compound. Endophytic fungi Diaporthe phaseolorum produces three types of active compounds as antibacterial namely phenol, terpenoids, and alkaloids, each component of the active compound are tested for sensitivity to bacterias Escherichia coli, Staphylococcus aureus, Shigella dysenteriae, and Salmonella typhi. MIC values ??of terpenoid compounds with concentrations of 125-250 ppm are antibacterial compounds with good activity against Escherichia coli, Staphylococcus aureus, Shigella dysenteriae, and Salmonella typhi.

Potency of endophytic fungi from Nauclea orientalis L. as antioxidant producer Elisa Nurnawati; Hary Widjajanti; Vivi Hendra Sutandar; Maulida Harwati; Eka Amelia; Siti Alharzsa; Nina Tanzerina
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 27 No 1 (2021): December 2021
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869//bphjbr.27.1.20216

Nauclea orientalis L. is widely distributed in Indonesia. Secondary metabolites such as flavonoids, polyphenols, and saponins are abundant in the bark of N. orientalis L. These compounds have the potential to act as antioxidants. Endophytic fungi, through genetic transfer and coevolution, can produce the same metabolites as their host plant. As a result, understanding the potential of endophytic fungi from N. orientalis L. to produce antioxidant compounds that can be developed is critical. According to the findings of this study, twelve isolates have the potential to produce secondary metabolites with antioxidant properties. Three isolates had high antioxidant activity: DB2 was identified as Aspergillus minisclerotigenes with an IC50 of 21 g/mL containing tannins, terpenes, and flavonoids, AB3 as Colletotrichum perseae with an IC50 of 31 g/mL containing tannins and terpenoids, and AB1 as Diaporthe tulliensis with an IC50 of 48 g/mL containing tannins. The secondary metabolite group has the potential to be developed into an antioxidant agent

Antioxidant Activity of Syzygium samarangense L. and Their Endophytic Fungi Budiono Budiono; Elfita Elfita; Muharni Muharni; Heni Yohandini; Hary Widjajanti
Molekul Vol 14, No 1 (2019)
Publisher : Universitas Jenderal Soedirman

Leaves of jambu air (Syzygium samarangense L.) has been used by local residents as medicine for various diseases caused by free radical agents in human’s body. This study aims to find a new source of antioxidants from medicinal plants and their endophytic fungi Syzygium samarangense leaves was fractionated by maceration method using gradient solvent i.e. n-hexane, ethyl acetate, and methanol. The antioxidant activity of the leaf fractions was determined using 1,1diphenyl-2-picryl hydrazyl (DPPH) method. The chemical compound was isolated from active fraction by chromatographic techniques and their chemical structure was identified using spectroscopy techniques. The endophytic fungi was isolated from S. samarangense leaves and continued to cultivation in potato-dextrose broth (PDB) medium for four weeks. The medium was extracted using ethyl acetate and determined its antioxidant activity. Fungi’s isolate with highest activity was analyzed to identify its molecular. Ethyl acetate fraction of S. samarangense leaves showed highest antioxidant activity. Spectroscopy analysis result concluded the isolated compound is 5,7-dihydroxy-6,8-dimethyl flavanone. Four endophytic fungi had been isolated form S. samarangense leaves. Antioxidant activity test showed that ethyl acetate extract of endophytic fungi BJA-1 has the highest value. Molecular identification of BJA-1 shows high homology with Lasiodiplodia venezuelensis strain CBS 129753.

POTENCY OF ENDOPHYTIC FUNGI ISOLATED FROM Muntingia calabura AS ANTIFUNGAL SUBSTANCES AGAINST Candida parapsilosis Agung Bimantara; Hary Widjajanti; Elisa Nurnawati
BIOVALENTIA: Biological Research Journal Vol. 8 No. 2 (2022)
Publisher : Biology Department, Faculty of Mathematics and Natural Sciences, Sriwijaya University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24233/biov.8.2.2022.226

Alternative is needed to overcome the problem of dandruff caused by C. parapsilosis. Endophytic fungi isolated from plants are capable of synthesizing compounds to produce various secondary metabolites. This study aimed to obtain the most active endophytic fungal isolates, determine the class of compounds, and determine the antifungal activity of the endophytic fungi (M. calabura) against C. parapsilosis. Fifteen isolates of endophytic fungi isolated from M. calabura were coded FDK1-FDK15. There was a color change in the media after 30 days of cultivation isolates of fungi. FDK4 and FDK13 have potential as antifungals. FDK13 fungal extract had the highest inhibition zone diameter of 20.00 mm. 20 eluates were obtained from the isolation of com-pounds (Thin Layer Chromatography), FDK 131, 1314, 1317, and 1320 eluates are compounds of the terpenoid group and FDK1316 eluates are phenol group compounds that are active in inhibiting yeast growth. FDK1317 was the most active compound isolate in inhibiting the growth of C. parapsilosis with an inhibition zone diameter of 12.5 mm.

Isolation and Potency Test of Sulfate Reducing Bacteria (SRB) as Bioremediation Agent for Ex-Coal Mining Soil Aria Yanka Paula Putri; Hary Widjajanti; Harminuke Eka Handayani
Sriwijaya Journal of Environment Vol 5, No 1 (2020): Environmental Friendly
Publisher : Program Pascasarjana Universitas Sriwijaya

Mining activities can have negative impact on the environment causing water and soil pollution. Chemical physical properties also biological environment to change condition to be low organic matter, low soil pH, low water holding capacity, inadequate nutrient and acid generating material. Coal mining land is acidic cause of sulfur oxidation reaction with rainwater to produce sulfuric acid so that acidic of the soil condition. Biotechnology study is a wise effort to solution environment pollution with Sulfate Reducing Bacteria (SRB) as bioremediation agents. SRB can contaminate sulphate with an indicator pH increase which indicates of a SRB population increase and sulfate content decrease. This study aims to obtain a SRB isolate as potential a bioremediation agent for ex-coal mining soil. This study is experiment research with SRB isolate as treatment, that obtained from ex-coal mining soil at PT. Bukit Asam Tanjung Enim, South Sumatera. The result of this study obtained 10 (ten) SRB isolated a from the bacterial isolation stage. Ten SRB isolates were selected, obtained by five SRB isolates that have black criteria with the number of colonies approaching the same so that it has the potential as a bioremediation agent for ex-coal mining soil. SRB isolates P10T1R2, P9T2R2, P6T1R3, P5T1R1 dan P2T1R2 were potential tested on ex-coal mining to increase pH and reduce sulfate. The P5T1R1 SRB isolate has the potential to reduce sulfate at a concentration of 3561 ppm. Furthermore the result of the 5% LSD test show that this isolates is significantly different that other isolates so that it can be used as bioremediation agent.

Co-Authors A. Wulandari A. Wulandari Abdul Madjid Agung Bimantara Agustina Alfarado, Daniel Alfia Rahma Kurniawati Ali Djamhuri Andika Puspita Dewi Angga Prasetya Mulya Aria Yanka Paula Putri Arum Setiawan Arum Setiawan Asiandu, Angga Puja Bambang Yudono Budiono Budiono Dade Jubaedah Dedi Rohendi Dedik Budianta E. Patriono E. Patriono Eka Amelia Elfita Elfita Elfita Elfita Elfita Elfita Elisa Nurnawati Ervina Mukharomah, Ervina Fitrya Fitrya Genti, Karta Sari Gusti Ayu Widayanti Handayani, Harminuke Eka Harminuke Eka Handayani Hary Wahyudi Heni Yohandini Laila Hanum Maraulina, Hotly Mardiyanto Mardiyanto Mardiyanto Maria Lusia Marini Wijayanti Maulida Harwati Meiyerani, Jeni Melki Melki Muharni Muharni Munawar Munawar Munawar Munawar Muslim Muslim Nabila Saraswati Neni Marlina Ni Luh Putu SR Nina Tanzerina Nita Aminasih Noviyanto NUNI GOFAR Nur Arifah Nurkayah, Nurkayah Oktiansyah, Rian Permana, Muhammad Aditya Putri, Amanda Rahmaniah Putri, Aria Yanka Paula Putri, Hanindita Alifia Rahmawati Ridwan Riris Aryawati Rosmania, Rosmania Rozirwan . Salni Salni Salni Saputra, Ade Bayu Sarno . Sarno Sarno Singgih Tri Wardana Siti Alharzsa Siti Yuliani Sri Pertiwi E Sutandar, Vivi Hendra Syarifah Tanbiyaskur Ummi Hiras Habisukan, Ummi Hiras Wuriesyliane Wuriesyliane Yustina Hapida, Yustina

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