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All Journal Proceedings of Annual International Conference Syiah Kuala University - Life Sciences & Engineering Chapter Journal of Stem Cell Research and Tissue Engineering Ovozoa: Journal of Animal Reproduction Jurnal Agro Veteriner
ERMA SAFITRI
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Agriculture, Biological Sciences & Forestry Engineering Health Professions Immunology & microbiology Medicine & Pharmacology Veterinary Other

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Pengaruh injeksi nikotin terhadap motilitas, viabilitas, dan integritas membran spermatozoa mencit (Mus Musculus) Kautsar Pandu Pramudita; Widya Paramita Lokapirnasari; Suheni Susilowati; Hermin Ratnani; Erma Safitri; Epy Muhammad Luqman
Ovozoa: Journal of Animal Reproduction Vol. 9 No. 3 (2020): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ovz.v9i3.2020.77-81

Abstract

The process of spermatogenesis is sensitive to nicotine, a main component of cigarette smoke. This study aimed to observe the effect of nicotine injection on motility, viability, and membrane integrity of the spermatozoa of mice (Mus musculus). The mice were 10 weeks old, weighing about 20 grams, and in healthy and fertile conditions. Mice were adapted for 1 week by being given feed in the form of pellets and drinking water every day. Twenty of the mice were divided into four groups. Group K as a control was injected with 0.9% NaCl at 2.5 mg/Kg bw. Group P1 was injected with nicotine at 2.5 mg/Kg bw. P2 was injected with nicotine at 5 mg/Kg bw. Group P3 was injected with nicotine at 10 mg/Kg bw. The injection was carried out subcutaneously as much as 0.5 ml once a day for 35 days. Data analysis used the Analysis of Variances (ANOVA), followed by Duncan's Multiple Range Test. The results showed that the viability, motility, and membrane integrity of the spermatozoa decreased significantly (p <0.05) according to the increase in the dose of nicotine injected. It could be concluded that subcutaneous nicotine injection linearly decreased the motility, viability, and membrane integrity of the spermatozoa of mice.
The Role of adaptive MSCs as an Attempt Regeneration of Spermatogenesis Process Using by Hypoxia Precondition In Vitro erma safitri
Journal of Stem Cell Research and Tissue Engineering Vol. 4 No. 2 (2020): JOURNAL OF STEM CELL RESEARCH AND TISSUE ENGINEERING
Publisher : Stem Cell Research and Development Center, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jscrte.v4i2.22752

Abstract

The aim of this research was to obtain to get adaptive MSCs by an treatment in hypoxia precondition in viro culture. Ini this research, hypoxia precondition was to be given 3% O2 concentration and compared to those of normoxia culture in O2 > 20%. Results showed that under 3% O2 concentration, CD105+ and CD45- by flowcytometri, immunocytokimia and immunofluorescence didn’t experience of change (undifferentiated). Meanwhile under > 20% O2 concentration, cells have experienced of change (not undifferentiated again), that was indicated with CD105+ become decrease and CD45- increase by flowcytometri, immunocytokimia and immunofluorescence. Conclusion, in this research showed that hypoxia precondition with 3% O2 concentration very support MSCs to constantly adaptive before transplantated for disturbance of spermatogenesis process, because did’nt experience become progenitor cells was not expectation (still undifferentiated).
Correlation of Body Condition Score and Parity in Dairy Cows that Experienced Repeated Breeding in Sendang, Tulungagung Pristian Devon Martyas Wahyuningrum; Pudji Srianto; Budiarto; Tita Damayanti Lestari; Herry Agoes Hermadi; Erma Safitri
Jurnal Agro Veteriner Vol. 7 No. 2 (2024): Journal Agro Veteriner
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/agrovet.v7i2.57623

Abstract

Repeat breeding is a syndrome which affected the reproduction and production efficacy of dairy cattle. Dairy cow is one of an animals which produce milk. This syndrome may cause high economic losses in dairy cattle farm. This research was conducted from February to March 2023. The aim of this research was to determine the correlation of body condition score and parity of repeat breeding on the dairy cow at Tani Wilis Cooperation, Sendang, Tulungagung. A total of 46 dairy cows that contributed to the repeat breeding were used in this study. Sampling was done by purposive sampling with the following condition the date AI and the date of parturition. This research was conducted in a survei with primary and secondary data collection. Primary data retrieval is done by interviewing farmers, body condition score of each dairy cow, and direct observation. As for the secondary data obtained by recording the card Artificial Insemination (AI) owned by inseminator. Data were analyzed using correlate statistics followed by Spearmann Test. The result of the research indicated that no significant difference (P>0,05) in body condition score and parity.
Uterine prolapse and related factors in beef cow at the Besuki Animal Health Center, Situbondo regency, East Java, Indonesia Kevin Mardotillah; Suzanita Utama; Erma Safitri; Herry Agoes Hermadi; Sri Pantja Madyawati; Djoko Legowo; Abdullah Hasib
Ovozoa: Journal of Animal Reproduction Vol. 13 No. 3 (2024): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ovz.v13i3.2024.146-152

Abstract

This study aims to determine the factors related to uterine prolapse in beef cow in the Besuki Animal Health Center working area in Situbondo regency. The sample consisted of 100 cows that calved in 2020-2022, whose data was obtained from the Department of Animal Husbandry of Situbondo regency, East Java, Indonesia. Determination of the months of the rainy and dry season was obtained from the Meteorological, Climatological and Geophysical Agency, Juanda Class I Meteorological Station. Other data regarding breed, age, parity of cow and dystocia incidence in cow was obtained by conducting interviews with farmers followed by confirmation with inseminator records, and direct observation of the housing system. The collected data were cross-tabulated and analyzed using Chi-square analysis to determine possible factors causing uterine prolapse. The results showed that the incidence of uterine prolapse in beef cow in the working area was 35% (35/100). The factors of housing, age, parity, and incidence of dystocia were significantly related (p <0.05) to the incidence of uterine prolapse, while breed and season did not have a significant influence (p > 0.05). It could be concluded that the age and parity of the cow at the time of calving, the practice of housing cow, and the incidence of dystocia were related to the incidence of uterine prolapse in cows in the Besuki Animal Health Center operational area.
Synergistic protective effects of α-tocopherol and zinc sulfate on superoxide dismutase activity and p53 expression in ovarian granulosa cells of lead-exposed female Wistar rats (Rattus norvegicus) iqbal dwi warsito; Suherni Susilowati; Erma Safitri; Ragil Angga Prastiya; Maya Nurwartanti Yunita; Amung Logam Saputro
Ovozoa: Journal of Animal Reproduction Vol. 14 No. 1 (2025): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ovz.v14i1.2025.16-24

Abstract

This study aimed to evaluate the protective effects of α-tocopherol and zinc sulfate on oxidative stress parameters in ovarian granulosa cells of female Wistar rats (Rattus norvegicus) exposed to lead acetate (Pb(C₂H₃O₂)₂). A total of 25 rats were randomly divided into five groups: Control (C), lead exposure only (T0), α-tocopherol + lead (T1), zinc sulfate + lead (T2), and α-tocopherol + zinc sulfate + lead (T3). Treatments were administered orally for 21 days. Superoxide dismutase (SOD) levels were measured spectrophotometrically, and p53 expression was analyzed using immunohistochemistry. The results showed that SOD levels significantly decreased in group T0 compared to the control (p <0.05), while treatment with either α-tocopherol (T1), zinc sulfate (T2), or their combination (T3) significantly improved SOD levels compared to T0. Moreover, p53 expression was markedly elevated in T0, indicating enhanced oxidative stress and potential apoptosis, whereas all antioxidant-treated groups showed reduced p53 expression, with T3 demonstrating values comparable to the control group. In conclusion, the combination of α-tocopherol and zinc sulfate provided a synergistic antioxidant effect, effectively enhancing SOD levels and suppressing p53 expression in granulosa cells of lead-exposed rats. This suggested a promising therapeutic potential of these compounds in mitigating lead-induced ovarian toxicity.
The effect of binder mycotoxins on the histopathology of broiler kidneys exposed to a combination of mycotoxins Anastasya, Jelita; Mirni Lamid; Poedji Hastutiek; Erma Safitri; Mohammad Anam Al-Arif; Hani Plumeriastuti
Jurnal Agro Veteriner Vol. 8 No. 2 (2025): Jurnal Agro Veteriner
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/agrovet.v8i2.74036

Abstract

This study was made to know the histopathological changes of kidney in broiler chicken fed by mix mycotoxin (aflatoxin B1 and ochratoxin A) contaminated feed. A total of 20 chickens aged DOC to 35 days was divided into 4 groups (K-, K+, P1 and P2) each group consisted of 5 chickens. Group K- as a control without the addition of the mix mycotoxin to the fed. Group K+ feed was added with 0.1 mg/kg of aflatoxin B1 and 0.1 mg/kg of ochratoxin A. Group P1 and P2 feed were added mix mycotoxin with the same dose with addition of 1.1 g/kg mycotoxin binder in P1 group and 1.6 g/kg of mycotoxin binder in P2 group. Treatment of contaminated feed has been given for 28 days from day 8 until day 35. At the end of the treatment period (day 35), chickens were euthanized, and histopathological examination was carried out. Kruskal-Wallis test showed a significant difference (p<0.05) on the mean rank of the kidney cell necrosis, cell degeneration, inflammatory cell infiltration, and haemorrhage. The conclusion of this study is that the administration of mycotoxin binder has an effect in preventing and reducing the necrosis, degeneration, inflammatory cell infiltration, and kidney haemorrhage.
Co-Authors . Helen . Mulyani . Purwati Abdullah Abdullah Abdullah Hasib Al arif, Mohammad Anam Anastasya, Jelita Budiarto Candra Bumi Dadik Raharjo Dian Ayu Permatasari Dilla Aranda Mayola Dirga Januar Surya Utama Djoko Legowo Emy Koestanti Sabdoningrum Epy Muhammad Luqman Fedik Abdul Rantam Hana Eliyani Hani Plumeriastuti Hardijanto Hardijanto Hermin Ratnani Herry Agoes Hermadi Imam Mustofa iqbal dwi warsito Ismudiono Ismudiono Kautsar Pandu Pramudita Kevin Mardotillah Koesnoto Supranianondo Mas’ud Hariadi Mirni Lamid Nanda Irsa Meydilasari Nunuk Dyah Retno Lastuti Poedji Hastutiek Prastiya, Ragil Angga Pristian Devon Martyas Wahyuningrum Pudji Srianto R. Heru Prasetyo Rimayanti Rimayanti Rimayanti Rochmah Kurnijasanti Rosiana Febrianila Rudi Irvanto Saputro, Amung Logam Sintya Kumalasari Wibowo Sri Hidanah Sri Mulyati Sri Pantja Madyawati Sri Wigati Mardi Suheni Susilowati Suherni Susilowati Sunaryo Hadi Warsito Suzanita Utama Tantri Nawangwulan Tatik Hernawati Tita Damayanti Tita Damayanti L Tita Damayanti Lestari Tjuk Imam R. Tjuk Imam Restiadi Tri Nurhajati Trilas Sardjito Vina Ari Prastyorini Wahyu Retno Swari Widya Paramita Widya Paramita L. Widya Paramita Lokapirnasari Wurlina, W Yunita, Maya Nurwartanti