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All Journal HAYATI Journal of Biosciences Buletin Agrohorti Jurnal Ilmu Pertanian Indonesia Jurnal Fitopatologi Indonesia Microbiology Indonesia BIOTROPIA - The Southeast Asian Journal of Tropical Biology Biogenesis: Jurnal Ilmiah Biologi Jurnal Hortikultura AGRIVITA, Journal of Agricultural Science Indonesian Journal of Biotechnology JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA Jurnal Entomologi Indonesia Makara Journal of Science Biodiversitas Journal of Biological Diversity
Abdjad Asih Nawangsih
Department Of Plant Protection, Faculty Of Agriculture, IPB University, Jl. Meranti, Kampus IPB Dramaga, Bogor, West Java, Indonesia 16680
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Veterinary

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Diversity of phyllospheric Actinomycetes in Liliaceae plants and their potential as growth inhibitors of Alternaria porri CHEPPY WATI; ABDJAD ASIH NAWANGSIH; ARIS TRI WAHYUDI; SURYO WIYONO; ABDUL MUNIF
Biodiversitas Journal of Biological Diversity Vol. 24 No. 10 (2023)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d241003

Abstract

Abstract. Wati C, Nawangsih AA, Wahyudi AT, Wiyono S, Munif A. 2023. Diversity of phyllospheric Actinomycetes in Liliaceae plants and their potential as growth inhibitors of Alternaria porri. Biodiversitas 24: 5234-5242. Actinomycetes are microorganisms belonging to a group of Gram-positive bacteria, that have the potential to act as biological control agents against various plant pathogens. Phyllospheric Actinomycetes particularly, can inhibit growth of various microorganisms, such as the fungus Alternaria porri Ell. Cif., which causes purple blotch disease in the shallot plants. These microbes thrive on the surface of leaves, particularly in plants belonging to Liliaceae family. Environmental conditions have been proven to greatly affect their diversity. Present investigation aims to determine diversity of phyllospheric Actinomycetes from Liliaceae plants and their potential to inhibit growth of A. porri. The methods used were to investigate, characterize, and quantify isolates from Liliaceae plants. An antagonistic test was conducted on the isolates to examine the proportion of A. porri fungus growth inhibition. The results showed that plants species Allium fistulosum and A. tuberosum were the most common hosts for phyllospheric actinomycetes. Morphological differences were found in shape, color, elevation, edges, surface, and hyphae size, as well as the type of spore violation. The surface texture of colonies was smooth, rough, powdery, and opaque, usually appearing after 7-12 days of isolation. The isolates also inhibited growth of A. porri, causing the mycelium thins, and a clear zone of inhibition between pathogens and Actinomycetes forms. Isolates BCW9 caused the highest suppression of fungus growth 57.78%. This study is the first experiment to evaluate the diversity of phyllosphere Actinomycetes from Liliaceae plants, which are able to control purple blotch disease caused by A. porri in Indonesia. The phyllospheric Actinomycetes were isolated from the same habitat of A. porri. These investigation show the prospect of phyllospheric Actinomycetes as biocontrol agents against another fungal diseases on leaves of another plants.
Glucanase activity produced by rhizospheric Streptomyces tritolerans ARJ 32 and Streptomyces collinus ARJ 38 and the analysis of their encoding genes DEMMA ZILBA BUDIMAN; WIDYA ESTI PURWANINGTYAS; JEPRI AGUNG PRIYANTO; IVAN PERMANA PUTRA; ABDJAD ASIH NAWANGSIH; ARIS TRI WAHYUDI
Biodiversitas Journal of Biological Diversity Vol. 24 No. 10 (2023)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d241065

Abstract

Abstract. Budiman DZ, Purwaningtyas WE, Priyanto JA, Putra IP, Nawangsih AA, Wahyudi AT. 2023. Glucanase activity produced by rhizospheric Streptomyces tritolerans ARJ 32 and Streptomyces collinus ARJ 38 and the analysis of their encoding genes. Biodiversitas 24: 5831-5837. The enzyme ?-1,3-glucanase is capable of breaking down the glucan components in the cell walls of phytopathogenic fungi. Streptomyces spp. isolated from the maize rhizosphere, are believed to produce enzymes with glucanolytic activity, making them promising candidates for use as a biological control agent against these fungi. This work aims to quantitatively analyze the enzymatic activity of glucanase in Streptomyces tritolerans ARJ 32 and Streptomyces collinus ARJ 38 and identify their encoding genes (bglS) and the three-dimensional modeling of their protein structures. The dinitrosalicylic acid (DNS) method quantitively assays the glucanase enzyme. The TA-Cloning of the bglS gene was performed using pGEM-T Easy Plasmid Vector, and the three-dimensional protein structure was constructed using the I-TASSER program. According to the findings, both Streptomyces isolates exhibited glucanolytic activity. The glucanase enzyme activities of S. tritolerans ARJ 32 and S. collinus ARJ 38 peaked at eight days of incubation, with values of 31.116 U/mg and 41.599 U/mg, respectively. The partial bglS gene was present in both Streptomyces and identified as endo-?-1,3-glucanase from the glycoside hydrolase family 16 (GH 16). The deduced partial amino acid sequences were aligned and showed some highly conserved residue in the catalytic domain of GH 16. The three-dimensional structural model built from the partial bglS amino acid sequences displayed high-quality parameters and overlapped with the protein model of partial endo-?-1,3-glucanase from Nocardiopsis sp. F96 (2HYK). These preliminary results suggest that the two Streptomyces isolates had the potential to be used as glucanase enzyme producers.
Variation in symptoms and morphology of Fusarium spp. on shallot associated with basal plate rot disease in Brebes District, Central Java Province, Indonesia LISA MARIANAH; ABDJAD ASIH NAWANGSIH; ABDUL MUNIF; GIYANTO GIYANTO; EFI TODING TONDOK
Biodiversitas Journal of Biological Diversity Vol. 25 No. 5 (2024)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d250538

Abstract

Abstract. Marianah L, Nawangsih AA, Munif A, Giyanto, Tondok ET. 2024. Variation in symptoms and morphology of Fusarium spp. on shallot associated with basal plate rot disease in Brebes District, Central Java Province, Indonesia. Biodiversitas 25: 2198-2208. Basal plate rot disease is an important disease of shallots that causes losses in the field and storage. The disease is caused by Fusarium complex species, with different levels of virulence and host susceptibility. The six species of Fusarium spp. reported to cause basal plate rot disease of onion were F. oxysporum f.sp. cepae, F. proliferatum, F. redolens, F. solani, F. acutatum and F. tricinctum. Information about the variation in symptoms and morphology of fungi that cause basal plate rot disease on shallots, especially in Indonesia, is still very limited. Besides, the species that cause basal plate rot disease on shallots has not been molecularly confirmed using specific primers. The objective of this study was to isolate, identify, and study the variation in symptoms of the fungus associated with basal plate rot disease. The fungi were isolated from shallot plants symptomatic of basal plate rot disease collected from Brebes, Central Java Province. Eight isolates of Fusarium with different morphologies were successfully isolated and identified. Five isolates were identified as F. solani (isolate BC1, BC2, BC3, BBS1, and BBS4), two isolates as F. oxysporum f.sp. cepae (isolate BC4 and BBS6) and one isolate F. proliferatum (isolate BBS5). All eight isolates were pathogenic on shallots with different levels of virulence and symptom variations. BC4 isolate had the highest level of virulence, resulting in plant death, and was identified as F. oxysporum f.sp. cepae.
Cellulolytic bacteria as biocontrol agents of chili basal stem rot disease caused by Phytophthora capsici FARHANTYO FAUZAN DWINUGROHO; GIYANTO GIYANTO; EFI TODING TONDOK; ABDJAD ASIH NAWANGSIH
Biodiversitas Journal of Biological Diversity Vol. 27 No. 1 (2026)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d270109

Abstract

Abstract. Dwinugroho FF, Giyanto, Tondok ET, Nawangsih AA. 2026. Cellulolytic bacteria as biocontrol agents of chili basal stem rot disease caused by Phytophthora capsici. Biodiversitas 27 (1): d270109. https://doi.org/10.13057/biodiv/d270109. Phytophthora capsici is a major pathogen that causes severe yield losses in chili cultivation. Its cellulose-rich cell wall presents an opportunity for biocontrol using cellulolytic bacteria capable of degrading cellulose. This study aimed to isolate cellulolytic bacteria with antagonistic activity against P. capsici and to evaluate their effectiveness in managing basal stem rot in chili. A total of 279 bacterial isolates were collected from diverse natural sources, of which 72 exhibited cellulolytic activity, with cellulolytic indices ranging from 0.22 to 10.42. Pathogenicity screening through hypersensitivity and hemolysis assays identified 26 non-pathogenic isolates. These isolates inhibited P. capsici growth by 8.15 to 79.81%. Selection of promising isolates was conducted using the Analytical Hierarchy Process (AHP), which considered both inhibition rate and plant growth-promoting traits (volatile organic compound [VOC] production, phosphate solubilization, nitrogen fixation, indole-3-acetic acid [IAA] production, and siderophore production. Based on this evaluation, five isolates were selected: S55, S61, K15, S80, and FS28, with inhibition rates of 79.81, 69.26, 57.78, 46.85, and 40.37%, respectively. In vivo greenhouse assays showed that S55 and K15 provided the highest plant survival rates (86.67 and 75.56%, respectively), compared to 50% in the control. Molecular identification revealed that S55 and S61 were closely related to Pseudomonas aeruginosa, K15 to Proteus penneri, FS28 to Bacillus altitudinis, and S80 to Brevundimonas sp. These findings demonstrate the potential of cellulolytic bacteria as biocontrol agents against P. capsici, while also highlighting the functional diversity of isolates with plant growth-promoting traits. This diversity expands the pool of candidates for sustainable and targeted disease management strategies in chili cultivation.
Screening, genetic diversity of aiiA gene in AHL-lactonase producing bacteria and their potential to suppress the virulence factors of Ralstonia syzygii subsp. indonesiensis Tiara Edelwinna; Giyanto Giyanto; Abdjad Asih Nawangsih
Jurnal Hama dan Penyakit Tumbuhan Tropika Vol. 25 No. 2 (2025): SEPTEMBER, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA: JOURNAL OF TROPICAL PLAN
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jhptt.225287-297

Abstract

Ralstonia syzigii subsp. indonesiensis (Rsi) is a causal pathogen of Phylotype IV within the Ralstonia solanacearum species complex, commonly found in chili plants. This pathogenic bacterium uses a quorum sensing (QS) mechanism that relies on N-acyl homoserine lactone (AHL) signals to regulate the expression of virulence genes, such as those encoding exopolysaccharides (EPS). Biological control of Rsi can be achieved by disrupting its QS system. The aim of this study was to isolate AHL-lactonase-producing bacteria, analyze the genetic diversity of their aiiA gene, and evaluate their effectiveness in suppressing EPS production in Rsi. The research involved sampling, isolating, and screening bacterial candidates from chili plants as AHL-lactonase producers using bioassays and molecular techniques, followed by evaluation of their ability to inhibit EPS expression as a virulence factor of Rsi. Bacterial samples were isolated from Brebes, Bandung, and Garut Regencies. Molecular identification revealed that the twelve selected isolates belonged to the genus Bacillus. Sequencing results showed genetic diversity in the aiiA gene among isolates obtained from regions with different altitudes. All isolates demonstrated the ability to suppress Rsi virulence factors.
Co-Authors , Giyanto . Giyanto Abdul Munif ABDUL MUNIF ABDUL MUNIF Abidin, Anugrawati Af'idzatuttama Af’idzatuttama Aisyah Imas Alina Akhdiya Alina Akhdiya Anak Agung Keswari Krisnandika Anja Meryandini Aris Tri Wahyudi Asysyuura, Asysyuura Boedi Tjahjono Budi Marwoto CHEPPY WATI Deden Sukmadjaja DEMMA ZILBA BUDIMAN Desta Wirnas DIMAS ANDRIANTO EDI HUSEN Efi Toding Tondok Eka Wijayanti Eka Wijayanti Ely Lailatul Maghfiroh Ely Maghfiroh Eny Widajati Erina Sulistiani FARHANTYO FAUZAN DWINUGROHO FAWWAZ DINANTY Firdaus, Muhamad Fajar Fitri Fatma Wardani Giyanto Giyanto Giyanto GIYANTO GIYANTO GIYANTO GIYANTO Gustan Pari Gustan Pari Haerani Haerani HANIFA NUR Fijrina Hanudin Hanudin HIMA DEWI Mariastuti Husda Marwan Ida Parida IKA DAMAYANTI Ika Roostika Iman Rusmana Imas Aisyah isnainy dinul Mursyalatiyus Ivan Permana Putra J.A. Priyanto Jepri Agung Priyanto Juang Gema Kartika Kikin H Mutaqin Krisnandika, A. A. Keswari LISA MARIANAH Marianah, Lisa MAYA SARI Meity S. Sinaga Meity Suradji Sinaga Meity Suradji Sinaga Meliah, Siti Muhammad Evan Nurrahmawan MUHAMMAD FAIZ AMRI Nahrisa Rahmadhani NAUFAL GHAZI FITHRIANSYAH Purwanto, Ukhradiya Magharaniq Safira RAHAYU FITRIANI WANGSA PUTRIE Sigiro, Ria Heni Siti Meliah Siti Zulaiha SRI MARTINA WIRASWATI SRI MARTINA WIRASWATI Sudir Sudir, Sudir Sulistiyani SURYO WIYONO Suryo Wiyono Suryo Wiyono Tiara Edelwinna Tita Widjayanti Tita Widjayanti . TRI ASMIRA DAMAYANTI Trikoesoemaningtyas UMI FATMAWATI Umi Fatmawati Wati, Cheppy WIDYA ESTI PURWANINGTYAS Wiraswati, Sri Martina Yana Anisa . YULIN LESTARI Zhenita Vinda Tri Handini