Article Per Year (5 Year)
p-Index From 2020 - 2025
2.447
P-Index
This Author published in this journals
All Journal Jurnal Sain Veteriner Buletin Veteriner Udayana Buletin Udayana Mengabdi Jurnal Veteriner Indonesia Medicus Veterinus Jurnal Medik Veteriner Jurnal Kedokteran Hewan Journal of Veterinary and Animal Sciences Jurnal Medika Veterinaria
Ida Ayu Pasti Apsari
Laboratorium Parasitologi Veteriner, Fakultas Kedokteran Hewan Universitas Udayana, Jl. PB. Sudirman Denpasar Bali, Indonesia 80225.
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Education Immunology & microbiology Medicine & Pharmacology Public Health Veterinary

Published : 54 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 54 Documents
Search

 2   3   4   5   6 
DETECTION OF SAG1 AND BAG1 Toxoplasma gondii DNA PROBES LABELLED WITH DIGOXIGENIN-11-dUTP Ida Ayu Pasti Apsari; Wayan Tunas Artama; Sumartono s; I Made Damriyasa
Jurnal Kedokteran Hewan Vol 7, No 1 (2013): March
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v7i1.579

Abstract

The objective of this research was to detect a minimum concentration of the probes that could be used for dot blot hybridization analysis. The method required labeled DNA probes. In this study a non-radioactive label of Digoxigenin-11-dUTP was used for labeling the Sag1 and the Bag1 of Toxoplasma gondii DNA probe. Labeling method for the probes was done according to the random primed labeling technique. The result showed that 0.67 pg/µl Sag1 probe and 0.58 pg/µl Bag1 probe could be detected by anti-Dig-antibody. It could be concluded that 0.67 pg/µl Sag1 probe and 0.58 pg/µl Bag1 probe could be used to diagnose toxoplasmosis by dot blot hybridization method.
6. In Vitro Insecticidal Activities of Ethanol Extract of Neem Leaves Against Heterodoxus Spiniger Lice In Dogs I Made Merdana; Monica Lewinsky; Ida Ayu Pasti Apsari
Jurnal Medika Veterinaria Vol 14, No 1 (2020): J.Med.Vet
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.med.vet..v14i1.16203

Abstract

Heterodoxus spiniger is a chewing lice that have been reported to infest dogs and cats. This ectoparasite is haematophagous and as an intermediate host in transmission of cysticercoids and egg of nematode worms. This study aims to determine the in vitro insecticidal activity of ethanol extract of neem leaf (Azadirachta indica) against H. spiniger. A total of 250 H. spiniger were divided into five groups with five replications, namely: P0 as a negative control group given placebo, P1 as a positive control group given deltamethrin 0.5EC 0.05%, and groups P2, P3 and P4 were given ethanol extract of neem leaves with concentrations of 2.5%, 5% and 10%. In each test, 10 lice samples were put into a petridish with a cotton swab that had been sprayed with the solution of each treatment, then the lice were treated again with fine spray evenly. The results showed that the in vitro ethanol extract of neem leaves had a very significant effect (P0.01) on the mortality of H. spiniger. The best results are shown at 5% concentration which is capable of killing 100% of lice in 30 minutes. It can be concluded that the ethanol extract of neem leaf has insecticidal activity against H. spiniger.
Prevalensi Protozoa Gastrointestinal Eimeria spp. Pada Sapi Bali Di Baturiti, Tabanan, Bali Hutapea, Maria Anastasia; Apsari, Ida Ayu Pasti; Kencana, Gst Ayu Yuniati
Indonesia Medicus Veterinus Vol 12 (4) 2023
Publisher : Faculty of Veterinary Medicine, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19087/imv.2023.12.4.517

Abstract

Ternak sapi potong merupakan salah satu komoditas peternakan yang menjadi tumpuan pemerintah Indonesia. Salah satu jenis sapi potong yang terkenal di Indonesia adalah sapi bali sehingga keberadaannya perlu dilestarikan karena merupakan plasma nutfah asli Indonesia. Upaya yang bisa dilakukan untuk melestarikan sapi bali adalah dengan menjaga kesehatan melalui pencegahan dan penanggulangan penyakit. Ternak sapi bali sangat mudah terinfeksi penyakit, salah satu di antaranya penyakit yang disebabkan oleh parasit. Infeksi parasit dapat mengakibatkan lambatnya pertumbuhan sapi terutama pada ternak muda. Salah satu infeksi parasit pada sapi adalah infeksi protozoa gastrointestinal. Infeksi protozoa gastrointestinal masih menjadi faktor yang mengganggu kesehatan sapi bali dan dapat menyebabkan kerugian ekonomi bagi peternak. Penelitian ini bertujuan untuk mengetahui prevalensi protozoa gastrointestinal Eimeria spp., yang menginfeksi sapi bali. Sampel penelitian berupa feses segar sapi bali berjumlah 60 sampel yang diambil di Kelompok Tani Suka Dharma Baturiti, Kabupaten Tabanan, Bali. Feses sapi yang berhasil dikumpulkan selanjutnya diperiksa dengan metode konsentrasi apung menggunakan larutan gula sheater. Prevalensi protozoa gastrointestinal dihitung dengan cara, jumlah sampel terinfeksi dibagi jumlah sampel yang diperiksa kemudian dikalikan 100%. Hasil penelitian didapatkan prevalensi protozoa gastrointestinal Eimeria spp. yang menginfeksi sapi bali di Baturiti, Tabanan, Bali adalah 28,33%.
Adaptation of African Swine Virus in Non-Swine Cell Lines: A Preliminary Study for Vaccine Candidate Tenaya, I Wayan Masa; Agustina, Kadek Karang; Suada, I Ketut; Apsari, Ida Ayu Pasti; Sari, Tri Komala; Handayani, Ni Made; Widayantari, Anak Agung Ayu Sauca Sunia; Suardana, Anak Agung Komang; Sumarya, I Made; Arsana, I Nyoman; Sudiartawan, I Putu; Wahyudi, I Wayan; Juliasih, Ni Ketut Ayu; Sudaryati, Ni Luh Gede; Damriyasa, I Made
Jurnal Medik Veteriner Vol. 8 No. 1 (2025): April
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol8.iss1.2025.114-123

Abstract

African Swine Fever (ASF) is among the most detrimental infectious viral diseases in pigs causing approximately 100% mortality. The disease was first reported about 83 years ago in Africa before spreading to Europe in 1957 and Asia in 2010. An adequate vaccine generally containing live attenuated virus isolates prepared in swine macrophages to control the disease is currently unavailable. Therefore, this study aimed to use murine neuroblastoma (N2a) cells, non-swine cell lines, to adapt African swine fever virus (ASFV) isolates for vaccine preparation. ASFV isolate called BL21 obtained from Bali and East Nusa Tenggara was previously propagated in swine macrophages. However, virus was currently adapted in the N2a cells to avoid unwanted issues associated with using swine macrophages, including microbial contamination, as well as technically laborious and ethical issues. The adapted BL21 was re-confirmed with quantitative polymerase chain reaction (q-PCR) and tested in vivo to examine the pathogenicity properties. The results showed that BL21 produced consistently and specifically positive q-PCR, killing experimental pigs with typical gross pathological changes of ASF. BL21 at a 10-3/mL dilution adapted in N2a cells showed similar antigenic properties causing the death of nearly 50% N2a cells in vitro and terminating all in vivo experimental pigs. In conclusion, the BL21 isolate reported in this study could be used as a vaccine candidate after more attenuation and particularly to determine a lethal dose of 50% (LD50) for future investigations.
Co-Authors Adven Three Any Joy Simamora, Adven Three Any Aini, Hanifah Alshofa Nurul Anak Agung Komang Suardana Anak Agung Sagung Indraswari Anak Agung Sagung Kendran Andi Azhary Azmy, Andi Azhary Ariawan, Kadek Yudha Aryitahlia, Ninis Chandra, Michele Dina, Putu Ayu DWI SURYANTO Dwipayana, I Putu Gilang Aria Endah Rahmawati Fuady Muslih Gusti Ayu Mayani Kristina Dewi Gusti Ayu Yuniati Kencana Handayani, Ni Made Handojo, Chindi Meilina Here, Rischi Robinson Male Hutapea, Maria Anastasia I Gusti Made Krisna Erawan I Gusti Ngurah Kade Mahardika I Ketut Berata I Ketut Suada I Made Damriyasa I Made Dwinata I Made Merdana I MADE SURAGA ARTA I NYOMAN ADI SURATMA I Nyoman Arsana, I Nyoman I NYOMAN MANTIK ASTAWA I Nyoman Suarsana I Nyoman Suartha I Nyoman Sulabda I Putu Sudiarta I Wayan Masa Tenaya I Wayan Masa Tenaya, I Wayan Masa I Wayan Wahyudi, I Wayan Ida Bagus Kade Suardana Ida Bagus Komang Ardana Ida Bagus Made Oka Ida Bagus Ngurah Swacita Ida Bagus Oka Winaya Ida Bagus Windia Adnyana Iwan Haryono Utama Kadek Karang Agustina Kencana, Gst Ayu Yuniati Ketut Ayu Cudemani Putri Komang Yogie Suryana Putra Leksana Aditya Kris Nugraha Madani, Inggrid Maria Mentari Ginting, Maria Mentari Mersy Rambu Maramba Ndiha Monica Lewinsky Muliani, Ni Kadek Ni Ketut Ayu Juliasih Ni Ketut Suwiti Ni Luh Gede Sudaryati Nina Aryani Pabala, Meiksilano Ferdy Pamungkas, Pandu Adjie Prawira, Satria Yuda Putu Agus Trisna Kusuma Antara Raisis Farah Dzakiyyah Al-Aliyya Saputri, Megawati Sianturi, Inti Sari Pati R U Simamora, Saruedi Sri Kayati Widyastuti Suastini, Ni Ketut Sudiartawan, I Putu Sugiyarto - Sumartono - Sumartono . Sumartono s Sumarya, I Made Suryani, Ni Made Putri Tjokorda Sari Nindhia TRI KOMALA SARI Wayan Tunas Artama Wayan Tunas Artama Widayantari, Anak Agung Ayu Sauca Sunia Widyasari, Ni Nyoman Ayu Yoshihiro Hayashi Zefanya, Fiorencia