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All Journal Jurnal Sain Veteriner Buletin Veteriner Udayana Buletin Udayana Mengabdi Jurnal Veteriner Indonesia Medicus Veterinus Jurnal Medik Veteriner Jurnal Kedokteran Hewan Journal of Veterinary and Animal Sciences Jurnal Medika Veterinaria
Ida Ayu Pasti Apsari
Laboratorium Parasitologi Veteriner, Fakultas Kedokteran Hewan Universitas Udayana, Jl. PB. Sudirman Denpasar Bali, Indonesia 80225.
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Education Immunology & microbiology Medicine & Pharmacology Public Health Veterinary

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Kejadian Balantidiosis pada Babi Landrace (A CASE STUDY OF BALANTIDIOSIS IN LANDRACE SWINE ) Ida Bagus Oka Winaya; I Ketut Berata; Ida Ayu Pasti Apsari
Jurnal Veteriner Vol 12 No 1 (2011)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

The aim of the study was to identify the incidence of balantidiosis in landrace pigs. A total of 60 pigswere examined at Faculty of veterinary medicine, Udayana University between January 2007 and January2008. Seven out of go pigs showed cahexia and diarrhoea . Macroscopic changes were observed, such as: thecolon was fully distended with gas and slight peritonitis,whereas microscopic examination revealed thepresence of Balantidium coli trophozoites and cysta within the intestinal mucosa. Additionally, enteritiskatarrhalis, slight hemorrhagis, erosin and pseudomembranous inflammation with lymphocytes andpolymorphonuclear cells were also noted.
BLOOD SMEAR EVALUATION OF BALI DUCKS SAMPLED FROM TRADITIONAL FARMING SYSTEMS IN BALI Iwan Haryono Utama; Sugiyarto -; Anak Agung Sagung Kendran; Ida Ayu Pasti Apsari; I Nyoman Suarsana; I Gusti Made Krisna Erawan; Anak Agung Ayu Mirah Adi; Ida Bagus Oka Winaya; Yoshihiro Hayashi
Jurnal Veteriner Vol 9 No 4 (2008)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Research to confirm the abnormalities of blood corpuscles in Bali ducks based on blood smear examination has been conducted. The research samples consisted of 105 ducks from various regencies in Bali. The blood smear examination was conducted by the method described in this literature and the data was collected and tabulated by means of the descriptive method. Erythrocyte abnormalities were: polychromasia (61.9%), anisocytosis (17.1%), poikilocytosis (21.0%). Abnormalities associated with leukocyte composition were: Lymphopenic (46.7%); Heterophylic (44.8%); Heteropenic (3.8%), also Thrombocyto penia (2.9%) was present in 105 ducks. In conclusion 61,9% ducks examined deal with regenerative anaemia. They also have some blood abnormalities such as basophylia, eosinophylia, heterophylia, heteropenia, lymphopenia, and monocytosis.
Sekuen Gen Surface Antigen-1 dan Bradizoit Antigen-1 Takizoit Toxoplasma gondii sebagai Kandidat Pemindai DNA (SAG1 AND BAG1 GENE SEQUENCES ANALYSIS OF TOXOPLASMA GONDII TACHYZOITE AS PROBE CANDIDATE) Ida Ayu Pasti Apsari; Wayan Tunas Artama; Sumartono .; I Made Damriyasa
Jurnal Veteriner Vol 13 No 4 (2012)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Sag1 and bag1 is a gene specific-stage for Toxoplasma gondii tachyzoite and bradyzoite. The purposeof this study was analyze the sequences of sag1 and bag1 tachyzoite genes of local of Toxoplasma gondiiisolate as deoxyribonucleic acid probe candidate. Tachyzoite of local of Toxoplasma gondii isolate used onthis study. Gene sag1 and bag1 gene of Toxoplasma gondii were amplified by PCR, and then sequenced. Theresults showed sag1 fragment gene contained 612 bp and bag 1 contained 470 bp in length. BLAST analysisof sag1 and bag1 gene fragments as probe candidate showed that high specific for Toxoplasma gondii andno significant cross-reaction fragment with host and other parasites. The sequences 136 bp and 98 bpfragments as DNA probe candidate of Toxoplasma gondii sag1 and bag1 respectively.
Produksi dan Karakterisasi Antibodi Monoklonal Anti-Cysticercus cellulosae (PRODUCTION AND CHRACTERIZATION OF MONOCLONAL ANTIBODIES AGAINST CYSTICERCUS CELLULOSAE) Ida Bagus Ngurah Swacita; I Made Damriyasa; Nyoman Sadra Dharmawan; Nyoman Mantik Astawa; Ida Ayu Pasti Apsari; Ida Bagus Made Oka; I Wayan Masa Tenaya
Jurnal Veteriner Vol 16 No 3 (2015)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

The purpose of this study is to make a monoclonal antibody against- Cysticercus cellulosae and itscharacterization. Samples antigen prepared from T. solium larvae (C. cellulosae) was then used to immunizeBalb/c. The immune response of mice assessed by ELISA test, then the lymphocytes of mice used for theproduction of monoclonal antibodies (MoAb). Origin lymphocytes of mice that produce antibodies againstC. cellulosae antigen, fused with myeloma cells (NS1). Results fusion of two cells produces hybrid cellscalled hybridomas; cells are then screened by ELISA test. Hybridoma cells that produce only MoAb, usedto produce large quantities in vitro. Characterization of MoAb against-C.cellulosae was tested by usingELISA and Western blotting. Mice were immunized with C.cellulosae antigen showed an immune responseproducing antibodies to C.cellulosae. Based on the results of fusion, produced a total of 51 hybridoma cellclones and after being screened, only three clones of hybridoma cells that produced MoAb against–C.cellulosae. MoAb produced, named after the hole where the growth of the ELISA micro plate, the BE6,BE7, and EE9. Characteristics of this MoAb capable of tracking cellulosae of fluid larvae and recognizeantigen protein bands with molecular weight 78kDa.
Respons Imun Mencit yang Diimunisasi dengan Cysticercus cellulosae (IMMUNE RESPONSE TO TAENIA SOLIUM CYSTICERCOSIS IN MICE) Ida Bagus Ngurah Swacita; I Made Damriyasa; Nyoman Sadra Dharmawan; Nyoman Mantik Astawa; Ida Ayu Pasti Apsari; I Wayan Masa Tenaya
Jurnal Veteriner Vol 16 No 2 (2015)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Cysticercosis is a zoonotic parasitic disease which is still problem in Indonesia. The purpose of thisstudy was to investigate immune responses of mice that had been immunized using Taenia solium larval(Cysticercus cellulosae) antigens originated from infected pigs. Three kinds of the C.cellulosaeantigens,secretory and whole antigens were used to immunize three different groups Balb/c mice consisted of 15animals. The serum samples before and after immunization were tested with ELISA test. The resultsshowed that the third antigens induced highly significant titre (P<0,01)compared to unimmunized animals.However no significant different (P>0,05) were found when the third antigens were compared. It wasconcluded that immunization with the three kinds of C. cellulosae antigens can cause immunity in mice.
Efektivitas Albendazol terhadap Cacing Nematoda Sapi Bali di Kelompok Tani Suka Dharma, Baturiti, Tabanan Raisis Farah Dzakiyyah Al-Aliyya; Ida Ayu Pasti Apsari; Gusti Ayu Yuniati Kencana
Buletin Veteriner Udayana Vol. 14 No. 5 October 2022
Publisher : The Faculty of Veterinary Medicine, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2022.v14.i05.p05

Abstract

This study aims to determine the effectiveness of albendazole against nematodes in bali cattle of farmer group Suka Dharma in Baturiti, Tabanan. Samples consists of 16 bali cattles which were divided into two groups, namely the control group that was not given albendazole therapy and the treatment group which was given albendazole at a dosage of 7,5 mg/kg of cattle’s weight orally. Faeces sampling was carried out on days 0, 7, 14, 21, and 28 post albendazole treatment. Samples were examined using the Wisconsin floating method to provide number of nematode eggs per gram faeces. Faecal Egg Counts Reduction was calculated to evaluate albendazole efficacy. The data obtained were further analyzed by Wilcoxon statistical tests. FECR values indicate that nematode worm eggs in bali cattle from the treatment group decreased by 91.55% on the 28th day post albendazole administration. Analysis by Wilcoxon test showed significant differences (P<0.05) between the number of nematode worm eggs in bali cattle before albendazole treatment and post albendazole treatment. It can be concluded that albendazole is effective against nematode worm infection in bali cattle of the Farmers' Group Suka Dharma, Baturiti, Tabanan.
Prevalensi dan Faktor Risiko Trichuris spp. pada Kambing yang Dipelihara di Kota Denpasar Ketut Ayu Cudemani Putri; Ida Ayu Pasti Apsari; Tjok Sari Nindhia
Buletin Veteriner Udayana Vol. 14 No. 6 December 2022
Publisher : The Faculty of Veterinary Medicine, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2022.v14.i06.p15

Abstract

Goats are animals that are susceptible to parasitic diseases, one of which is Trichuris spp. The purpose of this study was to determine the prevalence and risk factors for the infections of Trichuris spp. on goats kept in Denpasar city. In this study, 100 feces samples were taken from several breeders in the city of Denpasar, then data were recorded such as age, sex, number of goats per cage, breed, and also the housing system. The method used in this study is the floating method by taking 3 grams of feces, the homogenized and centrifuged at 1500 rpm for 5 minutes. Do it again after disposing of the supernatant. After centrifuged add saturated salt and let stand for 2-3 minutes and do the examination under the microscope and then the data were analyzed using bivariate analysis in the form of chi-square test. The result of this study found that the prevalence of Trichuris spp. by 95 and influenced by age and breed.
Dermatitis Suppurative Mengikuti Infeksi Tungau Demodeks dan Sarcoptes pada Anjing Kampung di Jalanan Kota Denpasar Ida Bagus Oka Winaya; Ida Bagus Windia Adnyana; I Ketut Berata; Ida Ayu Pasti Apsari
Jurnal Veteriner Vol 23 No 3 (2022)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19087/jveteriner.2022.23.3.328

Abstract

Tujuan penelitian ini adalah untuk mengetahui penyebab dermatitis dan kerontokan rambut pada anjing jalanan di Kota Denpasar. Dilakukan pencatatan terhadap identitas 75 ekor anjing yang meliputi umur, ras, tipe rambut dan lokasi lesi pada permukaan tubuh. Sampel biopsi kulit dengan ukuran 6 mm diambil dari lesi kulit yang paling parah dengan tanda klinis hiperemia, gatal, adanya krusta, bersisik/scaling, kebotakan/alopecia. Pengambilan sampel biopsi dilakukan dengan terlebih dulu memberikan injeksi anestetik lokal dan dilanjutkan dengan pemberian spraying antiseptik setelah pengangkatan jaringan biopsi. Sampel jaringan kulit kemudian difiksasi dalam larutan neutral buffered formalin 10%. Setelah 24-48 jam dalam cairan NBF jaringan diembedding dalam blok paraffin dengan metode standar. Sedian dengan ketebalan 5 mikron selanjutnya diwarnai dengan hematoksilin-eosin (HE). Pemeriksaan histopatologi terhadap tujuh ekor anjing penderita dermatitis dan kerontokan rambut umur di bawah satu tahun ditemukan kumpulan tungau demodek di beberapa folikel bulu. Eksudat radang netrofil dan eritrosit juga ditemukan pada permukaan epidermis yang nekrosis. Pada pemeriksaan terhadap 26 ekor anjing penderita dermatitis dan kerontokan bulu umur di atas satu tahun ditemukan 24 ekor anjing murni terinfeksi tungau demodeks dan dua ekor terinfeksi tungau demodeks dan sarcoptes. Ditemukan juga adanya larva tungau demodeks di luar folikel rambut. Larva tungau perifolikel terlihat dikelilingi oleh netrofil pada area dermis nekrosis, sedangkan pada anjing yang terinfeksi oleh dua jenis tungau hanya ditemukan respons radang ringan di sekitar folikel rambut.
DETECTION OF SAG1 AND BAG1 Toxoplasma gondii DNA PROBES LABELLED WITH DIGOXIGENIN-11-dUTP Ida Ayu Pasti Apsari; Wayan Tunas Artama; Sumartono s; I Made Damriyasa
Jurnal Kedokteran Hewan Vol 7, No 1 (2013): March
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (367.127 KB) | DOI: 10.21157/j.ked.hewan.v7i1.579

Abstract

The objective of this research was to detect a minimum concentration of the probes that could be used for dot blot hybridization analysis. The method required labeled DNA probes. In this study a non-radioactive label of Digoxigenin-11-dUTP was used for labeling the Sag1 and the Bag1 of Toxoplasma gondii DNA probe. Labeling method for the probes was done according to the random primed labeling technique. The result showed that 0.67 pg/µl Sag1 probe and 0.58 pg/µl Bag1 probe could be detected by anti-Dig-antibody. It could be concluded that 0.67 pg/µl Sag1 probe and 0.58 pg/µl Bag1 probe could be used to diagnose toxoplasmosis by dot blot hybridization method.
Tracking Antigen Expression of Toxoplasma gondii in Mice by Immuno (Cyto) Histochemistry Ida Ayu Pasti Apsari; Ida Bagus Oka Winaya; Tjokorda Sari Nindhia; Ida Bagus Ngurah Swacita
Journal of Veterinary and Animal Sciences Vol 1 No 1 (2017)
Publisher : Institute for Research and Community Service, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/JVAS.2017.v01.i01.p08

Abstract

Mouse has been an animal model that is very sensitive towards the Toxoplasma gondii infection. This research infects 30 mice with free-range chicken heart inoculate and the detection of T. gondiiby immunohistochemistry on the infected mice. The purpose of this study is to track T.gondii antigen expression by immune(cyto)histochemistry in mice. Observation over mice was conducted over 10 weeks. After 10 weeks, the mice are examined for its peritoneal fluid by microscopic and immunohistochemistry examination. Continued by the examination of internal organs to track the expression of T.gondii antigens by immunohistochemistry. The study result showed microscopic examinations on peritoneal fluid did not found any cyst or tachyzoites form of T.gondii. Examination of peritoneal fluid by immunohistochemistry was found to detect expressions of T.gondii antigen. Examinations of the mice’s internal organs detected expressions of T.gondii antigen only in the brain by immunohistochemistry examinations.
Co-Authors Adven Three Any Joy Simamora, Adven Three Any Aini, Hanifah Alshofa Nurul Anak Agung Komang Suardana Anak Agung Sagung Indraswari Anak Agung Sagung Kendran Andi Azhary Azmy, Andi Azhary Ariawan, Kadek Yudha Aryitahlia, Ninis Chandra, Michele Dina, Putu Ayu DWI SURYANTO Dwipayana, I Putu Gilang Aria Endah Rahmawati Fuady Muslih Gusti Ayu Mayani Kristina Dewi Gusti Ayu Yuniati Kencana Handayani, Ni Made Handojo, Chindi Meilina Here, Rischi Robinson Male Hutapea, Maria Anastasia I Gusti Made Krisna Erawan I Gusti Ngurah Kade Mahardika I Ketut Berata I Ketut Suada I Made Damriyasa I Made Dwinata I Made Merdana I MADE SURAGA ARTA I NYOMAN ADI SURATMA I Nyoman Arsana, I Nyoman I NYOMAN MANTIK ASTAWA I Nyoman Suarsana I Nyoman Suartha I Nyoman Sulabda I Putu Sudiarta I Wayan Masa Tenaya I Wayan Masa Tenaya, I Wayan Masa I Wayan Wahyudi, I Wayan Ida Bagus Kade Suardana Ida Bagus Komang Ardana Ida Bagus Made Oka Ida Bagus Ngurah Swacita Ida Bagus Oka Winaya Ida Bagus Windia Adnyana Iwan Haryono Utama Kadek Karang Agustina Kencana, Gst Ayu Yuniati Ketut Ayu Cudemani Putri Komang Yogie Suryana Putra Leksana Aditya Kris Nugraha Madani, Inggrid Maria Mentari Ginting, Maria Mentari Mersy Rambu Maramba Ndiha Monica Lewinsky Muliani, Ni Kadek Ni Ketut Ayu Juliasih Ni Ketut Suwiti Ni Luh Gede Sudaryati Nina Aryani Pabala, Meiksilano Ferdy Pamungkas, Pandu Adjie Prawira, Satria Yuda Putu Agus Trisna Kusuma Antara Raisis Farah Dzakiyyah Al-Aliyya Saputri, Megawati Sianturi, Inti Sari Pati R U Simamora, Saruedi Sri Kayati Widyastuti Suastini, Ni Ketut Sudiartawan, I Putu Sugiyarto - Sumartono - Sumartono . Sumartono s Sumarya, I Made Suryani, Ni Made Putri Tjokorda Sari Nindhia TRI KOMALA SARI Wayan Tunas Artama Wayan Tunas Artama Widayantari, Anak Agung Ayu Sauca Sunia Widyasari, Ni Nyoman Ayu Yoshihiro Hayashi Zefanya, Fiorencia