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All Journal Jurnal Sain Veteriner Buletin Udayana Mengabdi Jurnal Veteriner Indonesia Medicus Veterinus Jurnal Medik Veteriner Journal of Applied Veterinary Science and Technology Jurnal Kedokteran Hewan Journal of Veterinary and Animal Sciences Jurnal Medika Veterinaria Advances in Tropical Biodiversity and Environmental Sciences
Ida Ayu Pasti Apsari
Laboratorium Parasitologi Veteriner, Fakultas Kedokteran Hewan Universitas Udayana, Jl. PB. Sudirman Denpasar Bali, Indonesia 80225.
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Earth & Planetary Sciences Education Environmental Science Immunology & microbiology Medicine & Pharmacology Veterinary Other

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Prevalensi Protozoa Gastrointestinal Eimeria spp. Pada Sapi Bali Di Baturiti, Tabanan, Bali Hutapea, Maria Anastasia; Apsari, Ida Ayu Pasti; Kencana, Gst Ayu Yuniati
Indonesia Medicus Veterinus Vol 12 (4) 2023
Publisher : Faculty of Veterinary Medicine, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19087/imv.2023.12.4.517

Abstract

Ternak sapi potong merupakan salah satu komoditas peternakan yang menjadi tumpuan pemerintah Indonesia. Salah satu jenis sapi potong yang terkenal di Indonesia adalah sapi bali sehingga keberadaannya perlu dilestarikan karena merupakan plasma nutfah asli Indonesia. Upaya yang bisa dilakukan untuk melestarikan sapi bali adalah dengan menjaga kesehatan melalui pencegahan dan penanggulangan penyakit. Ternak sapi bali sangat mudah terinfeksi penyakit, salah satu di antaranya penyakit yang disebabkan oleh parasit. Infeksi parasit dapat mengakibatkan lambatnya pertumbuhan sapi terutama pada ternak muda. Salah satu infeksi parasit pada sapi adalah infeksi protozoa gastrointestinal. Infeksi protozoa gastrointestinal masih menjadi faktor yang mengganggu kesehatan sapi bali dan dapat menyebabkan kerugian ekonomi bagi peternak. Penelitian ini bertujuan untuk mengetahui prevalensi protozoa gastrointestinal Eimeria spp., yang menginfeksi sapi bali. Sampel penelitian berupa feses segar sapi bali berjumlah 60 sampel yang diambil di Kelompok Tani Suka Dharma Baturiti, Kabupaten Tabanan, Bali. Feses sapi yang berhasil dikumpulkan selanjutnya diperiksa dengan metode konsentrasi apung menggunakan larutan gula sheater. Prevalensi protozoa gastrointestinal dihitung dengan cara, jumlah sampel terinfeksi dibagi jumlah sampel yang diperiksa kemudian dikalikan 100%. Hasil penelitian didapatkan prevalensi protozoa gastrointestinal Eimeria spp. yang menginfeksi sapi bali di Baturiti, Tabanan, Bali adalah 28,33%.
Adaptation of African Swine Virus in Non-Swine Cell Lines: A Preliminary Study for Vaccine Candidate Tenaya, I Wayan Masa; Agustina, Kadek Karang; Suada, I Ketut; Apsari, Ida Ayu Pasti; Sari, Tri Komala; Handayani, Ni Made; Widayantari, Anak Agung Ayu Sauca Sunia; Suardana, Anak Agung Komang; Sumarya, I Made; Arsana, I Nyoman; Sudiartawan, I Putu; Wahyudi, I Wayan; Juliasih, Ni Ketut Ayu; Sudaryati, Ni Luh Gede; Damriyasa, I Made
Jurnal Medik Veteriner Vol. 8 No. 1 (2025): April
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol8.iss1.2025.114-123

Abstract

African Swine Fever (ASF) is among the most detrimental infectious viral diseases in pigs causing approximately 100% mortality. The disease was first reported about 83 years ago in Africa before spreading to Europe in 1957 and Asia in 2010. An adequate vaccine generally containing live attenuated virus isolates prepared in swine macrophages to control the disease is currently unavailable. Therefore, this study aimed to use murine neuroblastoma (N2a) cells, non-swine cell lines, to adapt African swine fever virus (ASFV) isolates for vaccine preparation. ASFV isolate called BL21 obtained from Bali and East Nusa Tenggara was previously propagated in swine macrophages. However, virus was currently adapted in the N2a cells to avoid unwanted issues associated with using swine macrophages, including microbial contamination, as well as technically laborious and ethical issues. The adapted BL21 was re-confirmed with quantitative polymerase chain reaction (q-PCR) and tested in vivo to examine the pathogenicity properties. The results showed that BL21 produced consistently and specifically positive q-PCR, killing experimental pigs with typical gross pathological changes of ASF. BL21 at a 10-3/mL dilution adapted in N2a cells showed similar antigenic properties causing the death of nearly 50% N2a cells in vitro and terminating all in vivo experimental pigs. In conclusion, the BL21 isolate reported in this study could be used as a vaccine candidate after more attenuation and particularly to determine a lethal dose of 50% (LD50) for future investigations.
DETECTION OF SAG1 AND BAG1 Toxoplasma gondii DNA PROBES LABELLED WITH DIGOXIGENIN-11-dUTP Apsari, Ida Ayu Pasti; Artama, Wayan Tunas; s, Sumartono; Damriyasa, I Made
Jurnal Kedokteran Hewan Vol 7, No 1 (2013): March
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v7i1.579

Abstract

The objective of this research was to detect a minimum concentration of the probes that could be used for dot blot hybridization analysis. Themethod required labeled DNA probes. In this study a non-radioactive label of Digoxigenin-11-dUTP was used for labeling the Sag1 and the Bag1 of Toxoplasma gondii DNA probe. Labeling method for the probes was done according to the random primed labeling technique. The result showed that 0.67 pg/l Sag1 probe and 0.58 pg/l Bag1 probe could be detected by anti-Dig-antibody. It could be concluded that 0.67 pg/l Sag1 probe and 0.58 pg/l Bag1 probe could be used to diagnose toxoplasmosis by dot blot hybridization method.
Landrace Swine Mortality Due to Colibacillosis in Conventional Farming Yasa, Gede Sastra Darma; Tono PG, I Ketut; Winaya, Ida Bagus Oka; Apsari, Ida Ayu Pasti; Sari, Tri Komala
Journal of Applied Veterinary Science And Technology Vol. 6 No. 2 (2025): October 2025
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/javest.V6.I2.2025.178-184

Abstract

Background: Colibacillosis, caused by Escherichia coli infection, is a major health issue in pig farming, leading to high morbidity and mortality rates. This report describes a case that occurred on a conventional pig farm in Puhu Village, Gianyar District, Bali. Purpose: This study aimed to determine the cause of death in a 43 day old Landrace pig exhibiting clinical signs including brown watery diarrhea, dehydration, and weight loss. Case(s): A necropsy was conducted at the Veterinary Pathology Laboratory, Faculty of Veterinary Medicine, Udayana University. Bacteriological examination was performed by culturing organ samples on Nutrient Agar and Eosin Methylene Blue Agar, followed by biochemical testing using Triple Sugar Iron Agar and Sulfide Indole Motility media, among others. Parasitological examination was carried out using the fecal float concentration method. Case Management: Epidemiological assessment revealed morbidity, mortality, and case fatality rates of 1.89%, 0.63%, and 33.33%, respectively. Gross pathological findings included intestinal and gastric dilation and hemorrhage, intestinal edema, and lesions in the brain, trachea, lungs, heart, liver, and kidneys. Histopathological examination revealed localized intestinal congestion, generalized cardiac congestion, and inflammatory cell infiltration in the intestinal mucosa. Bacteriological analysis confirmed Escherichia coli with alpha-hemolytic activity on blood agar. Eimeria spp. oocysts were not detected in the fecal samples. Conclusion:  The pig’s death was attributed to colibacillosis caused by Escherichia coli infection. This case highlights the importance of early detection, strict biosecurity measures, and proper sanitation practices to prevent disease outbreaks in conventional pig farming.
Phytochemical Test and Antioxidant Activity of Aqueous Extract of Marigold Flower I Made Merdana; Indira Laksmi Nandita; Pingkan Elia; Ida Ayu Pasti Apsari; I Wayan Gorda; Luh Made Sudimartini
Advances in Tropical Biodiversity and Environmental Sciences Vol. 8 No. 2 (2024): June 2024: Pages 58-118
Publisher : Institute for Research and Community Services Udayana University (LP2M Universitas Udayana)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/ATBES.2024.v08.i02.p08

Abstract

Natural medicines are increasingly in demand for safety reasons and are believed to be able to cure certain diseases. Literacy reveals that the marigold flower (Tagetes erecta L.) has medicinal potential as an antioxidant, anti-bacterial, anti-inflammatory, and anti-carcinogen. This research aimed to identify marigold flower aqueous extract's phytochemical content and antioxidant activity. The method used in this exploratory research was the maceration of marigold flower simplicia using distilled water for 24 hours, followed by evaporation to obtain marigold flower aqueous extract. Then, phytochemical screening and antioxidant bioactivity tests were carried out using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) method. The results of phytochemical screening showed that marigold flower water extract contained flavonoids, phenols, alkaloids, and tannins. Meanwhile, the antioxidant activity test obtained an IC50 value of 50.77 ppm. From these results, it can be concluded that the aqueous extract of marigold flowers has medicinal potential with vigorous antioxidant activity.
Co-Authors Adven Three Any Joy Simamora, Adven Three Any Aini, Hanifah Alshofa Nurul Anak Agung Komang Suardana Anak Agung Sagung Indraswari Anak Agung Sagung Kendran Andi Azhary Azmy, Andi Azhary Ariawan, Kadek Yudha Aryitahlia, Ninis Chandra, Michele Dina, Putu Ayu DWI SURYANTO Dwipayana, I Putu Gilang Aria Endah Rahmawati Fuady Muslih Gusti Ayu Mayani Kristina Dewi Gusti Ayu Yuniati Kencana Handayani, Ni Made Handojo, Chindi Meilina Here, Rischi Robinson Male Hutapea, Maria Anastasia I Gusti Made Krisna Erawan I Gusti Ngurah Kade Mahardika I Ketut Berata I Ketut Suada I Made Damriyasa I Made Dwinata I Made Merdana I MADE SURAGA ARTA I NYOMAN ADI SURATMA I Nyoman Arsana, I Nyoman I NYOMAN MANTIK ASTAWA I Nyoman Suarsana I Nyoman Suartha I Nyoman Sulabda I Putu Sudiarta I Wayan Gorda I Wayan Masa Tenaya I Wayan Masa Tenaya, I Wayan Masa I Wayan Wahyudi, I Wayan Ida Bagus Kade Suardana Ida Bagus Komang Ardana Ida Bagus Made Oka Ida Bagus Ngurah Swacita Ida Bagus Oka Winaya Ida Bagus Windia Adnyana Indira Laksmi Nandita Iwan Haryono Utama Kadek Karang Agustina Kencana, Gst Ayu Yuniati Ketut Ayu Cudemani Putri Komang Yogie Suryana Putra Leksana Aditya Kris Nugraha Luh Made Sudimartini Madani, Inggrid Maria Mentari Ginting, Maria Mentari Mersy Rambu Maramba Ndiha Monica Lewinsky Muliani, Ni Kadek Ni Ketut Ayu Juliasih Ni Ketut Suwiti Ni Luh Gede Sudaryati Nina Aryani Pabala, Meiksilano Ferdy Pamungkas, Pandu Adjie Pingkan Elia Prawira, Satria Yuda Putu Agus Trisna Kusuma Antara Raisis Farah Dzakiyyah Al-Aliyya Saputri, Megawati Sianturi, Inti Sari Pati R U Simamora, Saruedi Sri Kayati Widyastuti Suastini, Ni Ketut Sudiartawan, I Putu Sugiyarto - Sumartono - Sumartono . Sumartono s Sumartono s Sumarya, I Made Suryani, Ni Made Putri Tjokorda Sari Nindhia Tono PG, I Ketut TRI KOMALA SARI Wayan Tunas Artama Wayan Tunas Artama Widayantari, Anak Agung Ayu Sauca Sunia Widyasari, Ni Nyoman Ayu Yasa, Gede Sastra Darma Yoshihiro Hayashi Zefanya, Fiorencia