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All Journal MANAJEMEN HUTAN TROPIKA Journal of Tropical Forest Management ILMU KELAUTAN: Indonesian Journal of Marine Sciences Peronema Forestry Science Journal FORESTA BIOTROPIA - The Southeast Asian Journal of Tropical Biology SELAPARANG: Jurnal Pengabdian Masyarakat Berkemajuan Jurnal Ilmu dan Teknologi Kayu Tropis Sarwahita : Jurnal Pengabdian Kepada Masyarakat PERENNIAL Journal of Sylva Indonesiana ABDI MOESTOPO: Jurnal Pengabdian pada Masyarakat Prosiding Konferensi Nasional PKM-CSR Jurnal Sylva Lestari Repong Damar: Jurnal Pengabdian Kehutanan dan Lingkungan Jurnal Pengabdian Masyarakat Bangsa Global Forest Journal Journal of Fisheries & Marine Jurnal Penelitian Hasil Hutan

Arida Susilowati Iswanto

Center Of Excellence For Mangrove, Universitas Sumatra Utara, Medan, 20155. Indonesia

Author-ID : 232061

Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Civil Engineering, Building, Construction & Architecture Computer Science & IT Control & Systems Engineering Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Education Energy Engineering Environmental Science Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Nursing Physics Public Health Social Sciences Veterinary Other

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Implementation of 16S rRNA Gene for Fish and Shrimp Barcoding in Mangrove Ecosystems in North Sumatra and Aceh, Indonesia Hatika Hairani; Rizka Amelia; Ipanna Enggar Susetya; Arida Susilowati; Yuntha Bimantara; Tadashi Kajita; Mohammad Basyuni
Jurnal Ilmiah Perikanan dan Kelautan Vol. 15 No. 1 (2023): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.v15i1.36686

Highlight Research The genetic diversity of fish and shrimp from mangrove habitats was relatively substantial, with a heterozygosity of 0.926 and the exception of Langsa samples. AMOVA revealed that the diversity among individuals in the populations were higher compared to those within individuals. DNA barcode in mangroves is useful in developing mangrove conservation and restoration initiatives Abstract Fish and shrimp are among the species that are vulnerable to high level of disturbance in mangrove ecosystem. This study aimed to investigate the implementation of 16S rRNA gene for fish and shrimp barcoding in mangrove ecosystems in North Sumatra and Aceh, Indonesia. 50 samples fresh fish and shrimp were collected from five mangrove ecosystems in North Sumatra, namely Belawan, Lubuk Kertang, Percut Sei Tuan, and Pulau Sembilan and Langsa of Aceh for DNA extraction process. The research was divided into four activities: sample collection, DNA isolation, PCR amplification, and data analysis. DNA was extracted using kit extraction (kit Reliaprep gDNA Tissue Miniprep System) and with PCR amplification. Genetic parameters were analyzed using Gen Alex 6.51 software and the relationship between sites was examined using the MVSP 3.22. The genetic diversity of fish and shrimp from mangrove habitats was relatively substantial, according to the findings, with a heterozygosity of 0.926, with the exception of Langsa samples, which were only identified in subclass A1. Genetic relationship between sites revealed that all samples clustered into two branches and were randomly dispersed within each site. This work confirmed the 16S rRNA gene worked for fish and shrimp barcoding in mangrove ecosystems, North Sumatra and Aceh, Indonesia and our findings are expected to be useful in developing mangrove conservation and restoration initiatives.

Optimizing DNA Extraction and Selecting Suitable Regions for Biodiversity Assessment: A Study on Shorea leprosula Rachmat, Henti Hendalastuti; Yulita, Kusumadewi Sri; Dwiyanti, Fifi Gus; Susilowati, Arida; Arrofaha, Nawwall; Susila, Susila; Kamal, Irsyad; Siregar, Iskandar Zulkarnaen
Jurnal Manajemen Hutan Tropika Vol. 30 No. 1 (2024)
Publisher : Institut Pertanian Bogor (IPB University)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.7226/144

The extraction method plays a crucial role in obtaining high-quality DNA samples, which is indispensable for various molecular biology techniques and analyses, enabling a deeper comprehension of genetic information and biological processes. The objectives of the study were: a) to optimize the chloroplast DNA extraction protocol by comparing modified CTAB methods and GeneAid for both leaf and wood samples of Shorea leprosula, a major commercial timber species, and b) to identify a suitable cpDNA region that exhibits variability and universality across taxa. Total DNA was analyzed by gel electrophoresis followed by Sanger sequencing to determine the amplification success. The results revealed that trnL intron, trnL-trnF, and trnG yielded readable sequences of the expected length (maximum 586 bp, 480 bp, and 908 bp, respectively), while the rps 16 intron failed to assemble a contig. The petL-psbE region provided long readability for reverse sequences (769 bp) but not for the forward sequence (195 bp). Higher successful DNA extraction was achieved from the leaves compared to the woods. The lower sequencing quality may be attributed to suboptimal primer design, the structural features of the regions resulting from extensive repetitive sequences, and the suboptimal condition of the extraction method in eliminating wood chemical compounds.

Isolation and identification of cellulolytic fungi under Swietenia macrophylla, Mimusops elengi, and Polyalthia longifolia stands at the Universitas Sumatera Utara campus, Indonesia Muhammad Zyldanvi Bahri; Deni Elfiati; Arida Susilowati; Rizka Amelia
Global Forest Journal Vol. 1 No. 01 (2023): Global Forest Journal
Publisher : Talenta Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32734/gfj.v1i01.13251

The Universitas Sumatera Utara has various types of trees scattered in the surrounding environment. Swietenia macrophylla, Mimusops elengi and Polyalthia longifolia are the three most common tree species found on campus. To maximize the potential for utilizing tree species on the Universitas Sumatera Utara campus, several efforts can be made, one of which is by exploring the presence of cellulolytic fungi. Cellulolytic fungi are fungi that are able to hydrolyze cellulose which can produce cellulase enzymes. This study aims to obtain the potential and identify cellulolytic fungi from the soil under Swietenia macrophylla, Mimusops elengi and Polyalthia longifolia stands. Sampling was taken by making 3 plots on each type of stand measuring 20 m x 20 m with a soil depth of 0 â€“ 20 cm. In each plot, 5 sampling points were made. The soil taken from each type is then composed. Isolation of cellulolytic fungi was carried out using Carboxy Methyl Cellulose (CMC) media. The potenstial of cellulolytic fungi is obtain by calculating the cellulolytic index. All isolates obtained were identified morphologically down to the genus level. The result showed that the potency of the cellulolytic fungi ranged from 0.05 to 1.36. The identification results that the isolates included the genus Aspergillus and Trichoderma.

Moraceae diversity in the Universitas Sumatera Utara's arboretum Arida Susilowati; Deni Elfiati; Ida Mallia Ginting; Muhammad Irvan Rizmi
Global Forest Journal Vol. 2 No. 02 (2024): Global Forest Journal
Publisher : Talenta Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32734/gfj.v2i02.15573

The arboretum of the Universitas Sumatera Utara (USU) is an area for collecting and conserving tree species in North Sumatra. One of dominant family found in USU Arboretum is Moraceae.Â The objective of this reserach was to determine Moraceae diversity in the USU Arboretum. The census approach of vegetation analysis was used to determine the identity and abundance of Moracea. The research found 9 (nine) Moraceae species belongs to two genera in the USU arboretum, those were Ficus microcarpa, Ficus elastic, Artocarpus heterophyllus, Ficus padana, Ficus fistulosa, Artocarpus altilis, Ficus benjamina, Ficus ampelas and Ficus hispida. The species with the highest density value (57.92%) is Ficus hispida. The diversity index, richness index, and species dominance index were all quite low in the research location, with values of 1.455, 1.226, and 0.370, respectively. Meanwhile, the species evenness index at the research location is 0.662, which is classified into moderate. To improve the diversity of Moraceae in the USU arboretum, species enrichment activities are needed.

Adaptability of Kemenyan Toba (Styrax sumatrana) and Suren (Toona sureni) On Gold Mining Tailing Susilowati, Arida; Ahmad, Alfan Gunawan; Sirait, David Raymod
Journal of Sylva Indonesiana Vol. 2 No. 01 (2019): Journal of Sylva Indonesiana
Publisher : Talenta Publisher

Tailing was residue of gold mining process which lead to some environment problem. Beside containing heavy metal, tailing have lower soil fertility, physical, chemical and biological characteristics. To overcome this problem, revegetation activities was needed. The succesfulness of revegetation activities determined by adaptive trees selection. Local species was recommended because suitable to local climate and soil conditions, in this study we choosed toba benzoin (Styrax sumatrana) and suren (Toona sureni). The objective of this research was to get information about adaptability of toba benzoin and suren on growth media containing tailings. Research was conducted in green hause Faculty of Agriculture, University of Sumatera Utara. Factorial Randomized Completely Design with two factor were used in this research. The first factor was growing media (Factor A) and the second factor was seedling species (Factor B). The observed parameter were seedling height, seedling diameter, seedling dry weight, primary root lenght, shoot-root ratio and chemical properties of media. The results showed that the species and growing medium significantly affected the height growth of seedlings, seedling diameter, seedling dry weight and length of the primary root. The addition of topsoil and compost able to improve the chemical properties of the tailings are used. Tailings media and compost with composition ratio of 1:1 is recommended for Toba benzoin and Suren. That media provide the most optimal results of Toba benzoin and suren growth.

Land Cover Change and Deforestation Characteristics in The Management Section of National Park (MNSP) VI Besitang, Gunung Leuser National Park Sulistyono, Nurdin; Bastian Samuel P. Ginting; Pindi Patana; Susilowati, Arida
Journal of Sylva Indonesiana Vol. 2 No. 02 (2019): Journal of Sylva Indonesiana
Publisher : Talenta Publisher

Gunung Leuser National Park is one of the world heritage forest located in Indonesia where the Government of Indonesia and the International world give serious attention to the condition of the area. Unfortunately the forest area of Leuser Mountain National Park significantly decreasing by years due to deforestation.. This study aims to determine land cover changes, the rate of deforestation and spatial characteristics of deforestation in the National Park Management Section Region VI Besitang Gunung Leuser National Park. Classification method was maximum likelihood classification (MLC). The results of this study indicated land cover changes from 2008 to 2016 at SPTN Region VI Besitang TNGL that forest has increased from 104.741,15 ha to 107.336,03 ha, mixed dryland agriculture has decreased from 3.690,40 ha to 2.498,53 ha, Palm oil decreased from 526,96 ha to 88,40 ha, and open land increased from 3.116,80 ha to 3.572,93 ha. The rate of deforestation in this area during that period is 221,14 ha/year or 1.769,12 ha. Factors that have a close correlation with the area of deforestation are the number of population and the number of families farmer; distance from road, river, settlement and plantation; height class, and slope class.

The Study of Silviculture System for Selective Cutting and Line Planting (TPTJ) and Indonesian Selective Cutting and Planting (TPTI) Application at PT Intracawood Manufacturing Bulungan Regency, North Kalimantan M. Taufan; Susilowati, Arida
Journal of Sylva Indonesiana Vol. 3 No. 01 (2020): Journal of Sylva Indonesiana
Publisher : Talenta Publisher

Selective Cutting and Line Planting (TPTJ) silvicultural systems and Indonesian Selective Cutting and Planting (TPTI) are two of the five silvicultural systems that have been determined by the Ministry of Forestry Regulation No. P.11 / Menhut-II / 2009 juncto P.65 / Menhut-II / 2014 concerning silvicultural systems in the area of timber forest product utilization permits (IUPHHK) in production forests. Three other silvicultural systems based on the regulation are the Gap Logging silviculture system, the Fully Made Rejuvenation System, and the Multi Silviculture System. This study aims to examine the implementation of the TPTJ and TPTI systems in the PT Intracawood Manufacturing (PT Intraca) area, Bulungan Regency, North Kalimantan. The methodology of this research is through the approach of growth analysis, comparison of stand increment, observation of environmental conditions, interviews and literature study. Based on the field research, it was found that the diameter increments of Shorea leprosula, Shorea dasyphila, and Shorea parvifolia were greater in the TPTJ system compared to TPTI. However, the TPTI silviculture system at PT Intraca is easier to apply in the field than the TPTJ silviculture system. The TPTJ system has become less effective because planting in the TPTJ system has been carried out by making a 3-meter wide track with a length of approximately 1 km in a plot and if there is potential for the wood to become ineffective. In the area of PT Intraca, to improve the effectiveness of the TPTJ system a mosaic system is used where the planting path is made only in areas with less potential and representative for planting. The types of plants planted in the TPTJ system are far superior, perspective, and are faster than the types of plants in the TPTI system. Planting/enrichment in the TPTI system is easier because it does not need to make a planting path. Planting with seedlings from the extraction is only a transfer from the growth of tillers from areas with an excessive potential for tillers.

Pelatihan Monitoring Kesehatan Pohon: Upaya Konservasi Ruang Terbuka Hijau di Kota Pekanbaru Pebriandi, Pebriandi; Sahab, Ahmad; Mardhiansyah, Muhammad; Susilowati, Arida; Darlis, Viny Volcherina; Somadona, Sonia; Sribudiani, Evi; Oktorini, Yossi; Irfani, Ewi
Jurnal Pengabdian Masyarakat Bangsa Vol. 2 No. 7 (2024): September
Publisher : Amirul Bangun Bangsa

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.59837/jpmba.v2i7.1349

Monitoring kesehatan pohon adalah kegiatan pengamatan dan evaluasi berkala terhadap kondisi fisik pohon untuk memastikan bahwa pohon tersebut tumbuh dengan baik dan sehat. Tujuan utamanya adalah mendeteksi masalah kesehatan pada pohon, seperti penyakit, hama, atau gangguan lingkungan, serta memastikan langkah-langkah perawatan yang diperlukan diambil secara tepat waktu. Program pelatihan monitoring kesehatan pohon di Hutan Kota Pekanbaru bertujuan untuk meningkatkan keterampilan peserta dalam memelihara ekosistem hutan kota. Kegiatan ini melibatkan peserta dari Dinas Lingkungan Hidup dan Kebersihan kota Pekanbaru yang dilatih mengenai identifikasi penyakit pohon dan teknik perawatan dasar. Hasilnya menunjukkan peningkatan pengetahuan dan keterampilan peserta dalam memantau kesehatan pohon. Partisipasi aktif peserta mencerminkan kesadaran lingkungan yang lebih tinggi. Pelatihan ini diharapkan dapat menjadi langkah awal untuk program berkelanjutan dalam menjaga keberlangsungan hutan kota di kota Pekanbaru.

SIFAT-SIFAT PEREKAT TANIN FORMALDEHIDA DARI BAGIAN CABANG BAKAU HITAM (Rhizophora mucronata) : Properties of Tannin Formaldehyde Adhesive Made of Part of Branches Wood of Black Mangrove (Rhizophora mucronata) Nuryawan, Arif; Ritonga, Lia Anggraini; Basyuni, Mohammad; Risnasari, Iwan; Susilowati, Arida
Jurnal Penelitian Hasil Hutan Vol. 41 No. 3 (2023): Jurnal Penelitian Hasil Hutan
Publisher : BRIN Publishing

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jphh.2023.2564

Bakau hitam (Rhizophora mucronata) yang tumbuh di hutan mangrove bermanfaat dalam menahan abrasi dan tempat berkembangnya biota laut. Untuk mempertahankan fungsi ini dan supaya hutan mangrove tetap lestari, hanya bagian kayu cabang yang dimanfaatkan. Penelitian ini bertujuan untuk mengevaluasi bagian cabang bakau hitam untuk diekstraksi kandungan taninnya sebagai bahan baku perekat kayu. Pada penelitian ini dibedakan asal taninnya, yaitu dari bagian kulit, kayu, dan campuran kulit dan kayu yang diperoleh melalui ekstraksi menggunakan panci presto (pressure cooker) pada tekanan 10-15 psi selama 30 menit pada suhu 116-121°C. Metode sintesis meliputi pengeringan, konversi, ekstraksi tanin, pembuatan perekat tanin formaldehida, dan karakterisasi perekat tanin, yang meliputi kenampakan, pH, viskositas, berat jenis, kadar solid, waktu gel, dan kandungan emisi formaldehida. Hasil ekstraksi tanin menunjukkan rendemen tanin yang diperoleh pada ekstraksi kulit (9,32%) > kulit+kayu (5,95%) > kayu (3,28%). Oleh karena itu pembuatan perekat tanin formaldehida dan karakterisasinya berdasarkan Standar Nasional Indonesia 06–4567–1998 untuk resin phenol formaldehyde (PF) hanya dilakukan pada rendemen yang tertinggi yaitu kulit. Penampakan perekat tanin formaldehida asal kulit yang dihasilkan berupa cairan berwarna merah terang kehitaman, agak kental, terdapat butiran/kotoran, dan pH-nya 10,18 (basa). Kekentalannya 70,6 cps, berberat jenis 1,137, kadar solid 27,54%, periode gel 44 menit, dan kandungan formaldehida bebasnya 5,04%. Berdasarkan hasil penelitian ini dapat disimpulkan: 1) bagian cabang bakau hitam (R.mucronata) yang memiliki kandungan tanin tertinggi ada pada bagian kulit; 2) ekstraksi tanin menggunakan panci presto bisa diterapkan sehingga menghemat waktu ekstraksi; 3) Sifat-sifat perekat tanin formaldehida yang dihasilkan mirip dengan perekat PF hanya lebih encer.

Macropropagation of macadamia nut (Macadamia integrifolia Maiden & Betche) through shoot cuttings Arida Susilowati; Cut Rizlani Kholibrina; Mutya Kana Purba; Henti Hendalastuti Rachmat; Fifi Gus Dwiyanti; Ida Mallia Ginting
Global Forest Journal Vol. 3 No. 02 (2025): Global Forest Journal
Publisher : Talenta Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32734/gfj.v3i02.21225

Macadamia integrifolia, locally known as Macadamia, is a tree species native toQueensland, Australia. The species is also recognized as an appropriate option forland rehabilitation in Indonesia, with one location being the Lake Toba catchmentarea in North Sumatra. This species is planted on a large scale because of itsadaptability and relatively short harvesting time. The high demand for macadamiaseeds cannot be supported by the species' ability to regenerate, as naturalregeneration is slow due to the thick and hard seed coat. As a result, a short-term,quality propagation technique that the community can easily use, such as shootcuttings, is required. This study aimed to determine the success of the shoot cuttingtechnique and the adventitious roots development on macadamia cuttings. Thecutting material was obtained from a healthy mother tree from the Sipiso-pisogarden, North Sumatra. The research was designed by using the factorial completeblock design with two factors randomized design with two factors namely cuttingmedia (A) and auxin application (B). The media treatment consisted of sand andtopsoil mixture (A1: 1/1 v/v), pure sand (A2), and pure topsoil (A3), while theauxin application consisted of 0 g (B1), 200 ppm (B2), and 600 ppm (B3). Alltreatments were carried out with 3 replications, each consisting of 5 individualcuttings. The results showed that macadamia shoot cuttings varied survival rateranging from 33.33 % to 86.66% with the rooting percentage ranging from 6.66%-40%. The highest survival rate was found in the top soil mixture: sand and withoutauxin treatment (88.66%) while the lowest was found in sand and without auxin(33.33 %). The cutting media and auxin application did not significantly affect allthe observed parameters. Based on histological root observations, the adventitiousroots of Macadamia form from the wound-induced root with a process that beginswith callus formation and ends with the advent of adventitious roots. This researchfound that the juvenile level of the cutting material used is considered a veryimportant factor in the success of macadamia shoot cuttings.

Co-Authors Agung Wibowo Agus Purwoko Ahmad Baiquni Rangkut Ahmad Baiquni Rangkuti Ahmad Baiquni Rangkuti Alfan Gunawan Ahmad Ameilia Zuliyanti Siregar Aminuyati Anggita Amelati Harahap Anita Zaitunah Anita Zaitunah Apri H Iswanto Apri Heri Iswanto Arif NURYAWAN Arrofaha, Nawwall Atok Subiakto Attarik, Nuriskia Bastian Samuel P. Ginting Bejo Slamet Corryanti Corryanti Cut Rizlani Kholibrina Delvian Delvian Deni Elfiati Deni Elfiati Deni Elfiati Deni Elfiati Desrita Desrita Eling Tuhono Erman Munir Evi Sribudiani Ewi Irfani Fifi Gus Dwiyanti Fitra Yuda Pratama Fitra Yuda Utama Hafizah Arinah Hafizah Arinah Harefa, Meilinda Suriani Harisyah Manurung Hatika Hairani Henti Hendalastuti Rachmat Ida Mallia Ginting Imam Wahyudi Indrawan, Imam Wahyudi Ipanna Enggar Susetya Irsyad Kamal Iskandar Z Siregar Iskandar Z. Siregar ISKANDAR ZULKARNAEN SIREGAR Iskandar Zulkarnain Siregar Iwan RISNASARI Jajang Sutiawan Jajang Sutiawan Kansih Sri Hartini Kansih Sri Hartini KUSUMADEWI SRI YULITA Laswi Irmayanti Lestari, Andi Tri Luthfi Hakim Luthfi Hakim M. Basyuni M. Taufan Mariah Ulfa Mariah Ulfa Masitta Tanjung Melinda Suriani Harefa Moehar Maraghiy Harahap Mohammad Basyuni Muhammad Irvan Rizmi Muhammad Mardhiansyah Muhammad Zyldanvi Bahri Mutya Kana Purba Nelly Anna Nelly Anna Netti Herlina OK Hasnanda Syahputra OK Hasnanda Syahputra Pebriandi, Pebriandi Pelawi, Deanova Frestiana Br Pindi Patana Prijanto Pamoengkas Purwoko, Agus Rachmat, Henti Hendalastuti Ridwanti Batubara Ridwanti Batubara Rifqi Ulfah Nikmah Ritonga, Faujiah Nurhasanah Ritonga, Lia Anggraini Rizka Amelia Rizka Amelia Rudi Hartono Rudi Hartono Ryzki Pebriansyah Sahab, Ahmad Samsuri Samsuri Samsuri, Samsuri Sirait, David Raymod Somadona, Sonia Sucipto, Tito Sulistyono, Nurdin Supriyanto Supriyanto Supriyanto Supriyanto Suri Fadhilla Susila, Susila Tadashi Kajita Tadashi Kajita Tito Sucipto Tuhono, Eling Ulfa Juniarti Siregar Viny Volcherina Darlis Yossi Oktorini Yuntha Bimantara Zulkifli Nasution Zulkifli Nasution

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