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All Journal Agrointek Jurnal Pendidikan Biologi Pro Food JRST (Jurnal Riset Sains dan Teknologi) PERTANIAN TROPIK Contributions of Central Research Institute for Agriculture Prosiding Seminar Nasional Pengabdian kepada Masyarakat (SINAPMAS) Biosaintifika: Journal of Biology & Biology Education
Indra Kurniawan Saputra
Universitas Negeri Malang
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Engineering

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Eksplorasi Pati dari Limbah Kulit Singkong Sebagai Sumber Karbon Alternatif Media Pertumbuhan Rhizopus oligosporus Saito Assaajidah, Alima; Susanti, Evi; Saputra, Indra Kurniawan
Pro Food Vol. 11 No. 1 (2025): Pro Food (Jurnal Ilmu dan Teknologi Pangan)
Publisher : Fakultas Teknologi Pangan dan Agroindustri, Universitas Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/profood.v11i1.513

Abstract

Singkong (Manihot esculenta Crantz) merupakan makanan pokok penting bagi sekitar 800 juta orang, terutama di wilayah tropis, dengan peningkatan produksi global sekitar 100 juta ton sejak tahun 2000. Di Indonesia, konsumsi singkong yang meningkat turut menghasilkan limbah kulit singkong, yang umumnya dibuang atau digunakan sebagai pakan ternak bernilai ekonomi rendah. Pembuangan yang tidak dikelola dengan baik dapat menyebabkan pencemaran lingkungan dan emisi gas metana. Kulit singkong, dengan kandungan pati mencapai 36%, memiliki potensi sebagai media alternatif pertumbuhan mikroorganisme yang lebih murah dibandingkan media komersial seperti Potato Dextrose Agar (PDA). Penelitian ini bertujuan untuk memanfaatkan limbah kulit singkong sebagai media pertumbuhan Rhizopus oligosporus Saito, jamur yang digunakan dalam fermentasi pangan dan mampu menghasilkan metabolit sekunder bernilai tinggi. Maka dibutuhkan pengembangan media alternatif yang efisien dan ramah lingkungan untuk pertumbuhan mikroba. Metode penelitian meliputi karakterisasi R. oligosprus Saito, persiapan inokulum, pembuatan bubuk pati kulit singkong, serta optimasi konsentrasi pati kulit singkong, glukosa, dan pH media menggunakan fermentasi substrat cair (SmF). Hasil menunjukkan bahwa R. oligosprus Saito dapat tumbuh pada media alternatif berbasis kulit singkong dengan kondisi optimum pada 40 g/L pati kulit singkong, 10 g/L glukosa, dan pH 6 – 6.5. Penelitian ini menunjukkan potensi kulit singkong sebagai sumber karbon yang ekonomis dan ramah lingkungan, sekaligus mendukung pengembangan produk bioteknologi berbasis limbah organik.
Valorization of Oil Palm Empty Fruit Bunch Waste Enhanced with Molasses for Erythritol Production Setiabang, Jeffersen Hosea; Fildy, Michael Ryan; Limawan, Kezia Zefanya; Widiyono, Advent Roan; Yonas, Nathanael Darrell; Nataniel, Jocelyn; Saputra, Indra Kurniawan; Kembaren, Riahna
Biosaintifika: Journal of Biology & Biology Education Vol. 17 No. 2 (2025): August 2025
Publisher : Universitas Negeri Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v17i2.22551

Abstract

Indonesia, a leading palm oil producer, generates 1,250 tons of Oil Palm Empty Fruit Bunches (OPEFB) waste daily, typically disposed of through polluting combustion. This study explores repurposing OPEFB as a substrate for erythritol production, a popular sweetener with minimal impact on blood sugar. The research utilized Moniliella pollinis mutant SP5, a yeast capable of producing erythritol from various substrates. The process involved acid hydrolysis of OPEFB followed by fermentation. When fortified with 15% v/v molasses, OPEFB successfully served as an optimal carbon source for erythritol production, achieving a mass yield of 0.2878 g/g. Further improvement came through the use of ethyl methane sulfonate (EMS) mutagenesis, with the best mutant (mutant 7) reaching a yield mass of 0.3298 g/g and volumetric productivity of 0.0534 g/L/h. This research aims to advance Indonesia's self-sufficiency in erythritol production while providing a sustainable solution for OPEFB waste reduction, simultaneously addressing economic and environmental concerns.
Comparative Study of Bioinsecticide Quality of Bintaro Leaf (Cerbera odollam G.) between Laboratory and Pilot Scale Kurniawan, Indra Saputra; Susinggih Wijana2; Beauty Suestining Diyah Dewanti; Muji Paramuji
Jurnal Online Pertanian Tropik Vol. 12 No. 1 (2025): JURNAL ONLINE PERTANIAN TROPIK
Publisher : Talenta Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32734/jpt.v12i1.20019

Abstract

Bioinsecticide constitutes a sustainable solution to mitigate the deleterious consequences of excessive reliance on synthetic insecticides. Bintaro (Cerbera odollam Gaertn.) is a promising candidate for bioinsecticide production due to its efficacy. However, it is imperative to note that the scale of production significantly impacts the process conditions and the quality of the resulting products. To this end, a comparative study was conducted on the manufacturing of bintaro leaf bio-insecticide on a pilot scale. The objective of this study is to ascertain the discrepancy in saponin content, water content, and toxicity of bintaro extract between laboratory and pilot scale. This study uses the reference of previous research where the best treatment of material to solvent ratio 1:10 (w/v) was obtained, namely using 15 grams of bintaro leaf material and 150 ml of solvent with 7 cycles of socletation. In the pilot scale study there was an increase in the amount of material used to 2 kg of dried bintaro leaves and 30 liters of methanol solvent. The results showed that the crude extract of bintaro leaves had a yield of 27%, saponin content of 0.63%, water content of 19.35%, and toxicity level of 45.33%.
Co-Authors Abdillah, Himas Willya Putri Agus Dharmawan Ahmad Imam Nurdin Aisyah Khoirunnisa Alfi'ah, Nurul Anastacia Verena Salim Arik Anggara Assaajidah, Alima Azzahra, Fahda Bilqis Beauty Suestining Diyah Dewanti Devi Mariya Sulfa Dwi Listyorini Edy Mulyono Evi Susanti Fachrunnisa, Rifka Fatchur Rachman Febriandri Annisa Murti FEBRIANI, DWI Fildy, Michael Ryan Frida Kunti Setiowati Ghauts Mannah Aji Abdillah Gracela Noveline Christiana Telaumbanua Haliza, Fitri Nur Indradewa, Rhian Intan Chairun Nisa Kamaliya, Eliza Fitri Kembaren, Riahna Ken Raos Regina Kinasih, Sakinah Vinda Putri Kusbiantoro, Dedi Kusmayadi, Claresia Tsany Lea Assyifa Limawan, Kezia Zefanya Lubis, Fiqi Alfisar Mardiana Lelitawati Maryadi, Mohammad Ridho Dwi Muhamad Syaikhu Alam Muhibbatul Ma'rifah Muji Paramuji Nadila Sekar Zahida Nataniel, Jocelyn Norman Yoshi Haryono Novia Rahayu Nur'aini Kartikasari Nurlaily, Febyka Rahma Ratna Juwita Rini Nofrida Rizal Muhaimin Rizqullah Janitra Kristian Abadi Rozana, Kennis Setiabang, Jeffersen Hosea Sitoresmi Prabaningtyas Susinggih Wijana2 Tsania Wardah Maulidiyah Viska Rinata Widiyono, Advent Roan Yayuk Purwaningrum Yenni Asbur Yonas, Nathanael Darrell Yunita Rakhmawati Zainuri Zainuri