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All Journal HAYATI Journal of Biosciences Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vegetalika Jurnal Ilmu Pertanian EUGENIA Zuriat Jurnal Ilmiah Aplikasi Isotop Radiasi BIOTROPIA - The Southeast Asian Journal of Tropical Biology Indonesian Journal of Agricultural Science AGRIVITA, Journal of Agricultural Science Jurnal Agroekoteknologi Indonesian Journal of Biotechnology Jurnal Perlindungan Tanaman Indonesia Journal of Tropical Biodiversity and Biotechnology Jurnal Bioteknologi & Biosains Indonesia (JBBI) Jurnal Pertanian Agros jurnal Ilmu Pertanian (Agricultural Science) Sains dan Matematika Agroista: Jurnal Agroteknologi BIO-SAINS | Jurnal Ilmiah Biologi Indonesian Journal of Jamu Jurnal Ilmu-Ilmu Pertanian
Aziz Purwantoro
Universitas Gadjah Mada
Agriculture, Biological Sciences & Forestry Astronomy Biochemistry, Genetics & Molecular Biology Chemistry Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Education Environmental Science Health Professions Immunology & microbiology Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Physics Veterinary

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Journal : jurnal Ilmu Pertanian (Agricultural Science)

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Evaluasi Daya Gabung Karakter Hasil dan Komponen Hasil Lima Galur Mentimun Gungun Wiguna, Aziz Purwantoro, dan Nasrullah
Jurnal Ilmu Pertanian Vol 16, No 1 (2013): Juni
Publisher : Faculty of Agriculture, Universitas Gadjah Mada jointly with PISPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ipas.2524

Abstract

INTISARIPendugaan daya gabung umum tetua dan daya gabung khusus persilangan diperlukan sebagai pedoman untuk memilih tetua secara efektif dalam program hibridisasi. Penelitian bertujuan untuk menduga nilai daya gabung lima galur mentimun hasil persilangan berdasarkan rancangan dialel metode 2 model 1 menurut griffing. Hibridisasi dilakukan di Lembang dari bulan Oktober 2011 hingga Mei 2012. Evaluasi tetua dan F1 dilakukan di Lembang dan Subang dari bulan Juli hingga Oktober 2012, mengunakan Rancangan Acak Kelompok Lengkap dengan tiga  ulangan  pada  tiap  lokasi. Hasil penelitian menunjukkan DGU dan DGK sangat berbeda nyata untuk semua karakter. Interaksi DGU×lokasi sangat nyata untuk karakter panjang buah dan diameter buah, serta nyata pada karakter berat per buah. Interaksi DGK×lokasi  sangat nyata untuk karakter diameter buah. Galur P1 memiliki nilai daya gabung umum terbaik untuk karakter berat buah per tanaman. Galur P3 memiliki  nilai  daya  gabung umum terbaik  untuk  karakter jumlah buah per tanaman. Kombinasi persilangan yang memiliki nilai DGK tinggi untuk karakter hasil dihasilkan oleh hibrida P1×P2, P1×P5, P2×P5, dan P3×P4.Kata kunci : mentimun, DGU, DGK
Induksi Ketahanan Tanaman Jagung (Zea mays L.) Terhadap Penyakit Bulai Melalui Seed Treatment Serta Pewarisannya pada Generasi S1 Hoerussalam, Aziz Purwantoro, dan Andi Khaeruni
Jurnal Ilmu Pertanian Vol 16, No 2 (2013): Desember
Publisher : Faculty of Agriculture, Universitas Gadjah Mada jointly with PISPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ipas.2532

Abstract

INTISARIKetahanan terhadap penyakit merupakan salah satu sifat yang sangat penting dalam pemuliaan tanaman karena mempengaruhi kualitas dan tingkat produksi tanaman. Salah satu upaya untuk meningkatkan ketahanan tanaman terhadap penyakit adalah melalui induksi ketahanan sistemik yang dipicu oleh pengaplikasian elisitor dengan melibatkan koordinasi dan ekspresi dari gen tertentu (gen SAR) serta ditandai oleh akumulasi senyawa tertentu seperti asam salisilat atau asam jasmonat.Penelitian terdiri dari tiga bagian percobaan, yaitu: 1. Seleksi galur. Percobaan ini bertujuan untuk mendapatkan galur yang mengalami peningkatan status ketahanan dan memilih satu dari enam galur yang paling responsif terhadap perlakuan (elisitor). Percobaan menggunakan enam varietas jagung hibrida C02, C05, C13, C19, C20 dan SC 4D-139 yang diaplikasikan empat macam elisitor, yaitu Plant Growth Promoting Rhizobakteri (PGPR) Bio1 dan Bio2, asam salisilat (Abio1), serta Benzothiadiazole-S-Methyl (Abio2) melalui seed treatment. Percobaan dilakukan di lapang dengan menggunakan tanaman penyebar (spreader) sebagai sumber inokulum. Benih yang sudah di treatment kemudian ditanam, dan diamati sampai umur 42 hari setelah tanam. Evaluasi perubahan status ketahanan dilakukan dengan cara membandingkan status ketahanan asal (non treatment) dengan status ketahanan setelah diinduksi. Tanaman dari varietas yang mengalami peningkatan status ketahanan akan di selfing untuk mendapatkan benih generasi S1. 2. Status ketahanan terinduksi yaitu verifikasi ketahanan terimbas di tingkat fisiologis dan molekuler melalui pengukuran asam salisat dan deteksi gen PR-1 menggunakan teknik PCR. 3. Evaluasi pewarisan ketahanan pada generasi hasil selfing (S1) dari varietas yang mengalami peningkatan status ketahanan.Hasil menunjukkan jagung galur C20 paling responsif terhadap keempat macam elisitor dan mengalami peningkatan status dari agak rentan menjadi agak tahan ( perlakuan Bio1 dan Abio1) dan menjadi tahan (perlakuan Bio2 dan Abio2). Verifikasi secara fisiologis dan molekular menunjukan bahwa kandungan asam salisilat cenderung mengalami peningkatan setelah inokulasi P. maydis dibandingkan dengan sebelum inokulasi patogen dan terdeteksi gen PR-1 pada tanaman dari varietas C20 hasil treatment. Sementara itu, analisis studi pewarisan menunjukkan peningkatan ketahanan galur jagung C20 diturunkan pada populasi generasi S1 dan mengikuti pola pewarisan Mendel untuk rasio 15:1.Kata kunci: induksi ketahanan, elisitor, asam salisilat, PGPR, pathogenesis-related protein
Pengaruh Giberelin Terhadap Karakter Morfologi dan Hasil Buah Partenokarpi pada Tujuh Genotipe Tomat (Solanum lycopersicum L.) Agus Budi Setiawan; Rudi Hari Murti; Aziz Purwantoro
Jurnal Ilmu Pertanian Vol 18, No 2 (2015): August
Publisher : Faculty of Agriculture, Universitas Gadjah Mada jointly with PISPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ipas.6521

Abstract

Giberelin merupakan zat pengatur tumbuh yang berperan dalam pertumbuhan dan perkembangan buah tomat. Buah partenokarpi dapat diinduksi dengan menggunakan giberelin. Penelitian ini bertujuan untuk menentukan respon tujuh genotipe tomat terhadap GA3 terkait karakter fruit set, ukuran, dan hasil buah tomat. Penelitian ini dilakukan di Green House UPTD Balai Pengembangan Perbenihan Tanaman Pangan dan Hortikultura, Dinas Pertanian Pemerintah Daerah Istimewa Yogyakarta serta Laboratorium Genetika dan Pemuliaan Tanaman, Fakultas Pertanian, UGM mulai bulan Oktober 2014 hingga Februari 2015. Penelitian menggunakan rancangan faktorial 7 genotipe x 2 konsentrasi GA3 yang disusun dalam rancangan acak kelompok lengkap dengan 3 blok. Kluster bunga dengan bunga pertama fase 12 yang tidak dikastrasi disemprot GA3 dengan interval 3 hari sekali sebanyak 6 kali. Hasil penelitian menunjukkan bahwa B78 merupakan genotipe yang responsif terhadap GA3dengan ditandai dengan peningkatan jumlah lokul menjadi 6 (lokul/buah) danmengalami penurunan fruit set sebesar 81,96% serta ukuran buah yang menurun secara nyata dibandingkan dengan buah berbiji. Genotipe yang tanggap terhadap aplikasi GA3 untuk menginduksi buah partenokarpi dengan hasil dan ukuran buah yang bagus adalah Gamato 1 ditandai dengan penurunan bobot buah per tandan yang relatif kecil yaitu 28,38% serta buah partenokarpi yang dihasilkan memiliki ukuran panjang dan diameter buah yang masih jauh lebih besar (41,68 mm dan 46,11 mm) dibandingkan genotipe lainnya. Buah partenokarpi A65, Gamato 3, A175, Gamato 5 dan Kaliurang 206 mengalami penurunan ukuran (panjang, diameter, dan ketebalan daging buah) serta penurunan hasil buah tomat dibandingkan dengan buah berbiji.
Diversity Analysis of 15 Hibiscus Accession Based on RAPD Marker Miranda Ferwita Sari; Aziz Purwantoro
Jurnal Ilmu Pertanian Vol 3, No 2 (2018): August
Publisher : Faculty of Agriculture, Universitas Gadjah Mada jointly with PISPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ipas.33211

Abstract

Genus Hibiscus consist of 300 species in tropical and subtropical regions. Indonesia has many species of Hibiscus genus such as Hibiscus rosa-sinensis, kenaf, rosella, waru, sharon, and others. These species have similar flower morphology despite their different benefits. Flower morphology can be use as morphological marker characters to identify the genetic relationship in one genus of Hibiscus. However, morphological markers are less accurate because they are strongly influenced by the environment, requiring quite amount of time, and showing limited and inconsistent diversity. These limitations make researcher chooses molecular markers that are considered more accurate because the material used is plant DNA. One of the most used molecular marker is Random Amplified Polymorphic DNA (RAPD). This study used DNA extraction from the leaf of 15 Hibiscus genus. The next steps were DNA quantification, temperature and primer optimization, DNA amplification with PCR, electrophoresis with agarose gel, and data analysis. The result showed 10 primers operon used for Hibiscus to produce many polymorphic bands. Analysis result showed a high diversity in control population (Hibiscus cannabinus (K1), Hibiscus sabdariffa (K2) Hibiscus mutabilis (K3), Hibiscus syriacus (K4), Hibiscus schizopetalus (K5)) while in Hibiscus rosa-sinensis, the diversity was low especially on the genotypes of flowers with the same color. Molecular marker is a right way to identify the diversity in a population. Control group which consist of Hibiscus cannabinus, Hibiscus sabdariffa, Hibiscus mutabilis, Hibiscus syriacus and Hibiscus schizopetalus has high heterozygosity means control group has high diversity. From the PCA result, grouping in control plants is based on their age (perennial and annual), while in Hibiscus rosa-sinensis the grouping is based on the flowers color. Hibiscus rosa-sinensis is cross pollinated plants caused the position stigma higher than anthers, its prooved by this experiment that varians within population Hibiscus rosa-sinensis higher than among population.
The Effect of Drying and Storage on the Quality of Shallot (Allium cepa L. Aggregatum group) Bulbs Rohimah Handayani Sri Lestari; Endang Sulistyaningsih; Aziz Purwantoro
Jurnal Ilmu Pertanian Vol 3, No 3 (2018): December
Publisher : Faculty of Agriculture, Universitas Gadjah Mada jointly with PISPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ipas.34203

Abstract

Post-harvest handling in shallot such as drying of bulbs can influence its quality during and after storage. The objective of this study was to determine the impact of drying and storage treatment on the quality of shallot bulbs during 12 weeks of storage . The study was carried out in Samiran hamlet, Parangtritis village, Bantul district, Special Region of Yogyakarta and Crop Science Laboratory of the Faculty of Agriculture, Universitas Gadjah Mada, from June until November 2016. It was arranged in factorial randomized complete block design (RCBD) and consisted of two factors. The first was the drying treatments: drying the bulbs on the field and on woven bamboo nets both plastic covered and uncovered. The second was the storage treatments: storing the bulbs in the farmer’s warehouse (31,030C±0,04 and RH of 60,50%±0,28), in air-conditioned room (22,40oC± 0,02 and RH of 61,60%±0,09), and at room temperature (30,47oC±0,03 and RH of 60,50%±0,12). Each treatment combination was replicated three times as blocks. The results showed that water content of all treatments were changing followed by fluctuating of the total soluble solid throughout the storage period while bulb firmness tended to decrease. Bulbs which were stored in air-conditioned rooms showed the highest percentage of sprouted bulbs, vigor index and germination rate than other treatments. Meanwhile, drying treatment did not give significant influence.          
Telomere Length Determination Based on Different Sex and Leaf Development of Snake Fruit (Salacca zalacca GART. VOSS.) Revealed by Polymerase Chain Reaction Rima Indhirawati; Aziz Purwantoro
Jurnal Ilmu Pertanian Vol 3, No 3 (2018): December
Publisher : Faculty of Agriculture, Universitas Gadjah Mada jointly with PISPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ipas.36172

Abstract

Telomere has special roles at the ends of eukaryotic chromosomes that consisting of repeated DNA sequences. It protects chromosomes and DNA from damage. The plant sex and the leaf development may change in telomere length. Snake fruit (Salacca zalacca GART. VOSS.) is dioecious plants that have female and male organs on separate plants. The aim of this research is to determine the telomere length of snake fruit from different plant sex and the leaf development. In this research, we observed telomere length in snake fruit (female and male plants) using Polymerase Chain Reaction (PCR) procedure. The results from this study showed that telomere lengths of male and female are equal. Telomere lengths in the leaf development showed that younger leaves of both male and female leaves are longer compared to older and dried leaves.
Genetic Diversity of Croton (Codiaeum variegatum (L.) Rumph.ex A. Juss) and Its Offspring Based on RAPD Markers Mufit Daryatun Asniawati; Aziz Purwantoro
Jurnal Ilmu Pertanian Vol 4, No 2 (2019): August
Publisher : Faculty of Agriculture, Universitas Gadjah Mada jointly with PISPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ipas.40899

Abstract

Croton (Puring) is a native  plant of Indonesia which has varied leaf shapes and colors. The diversity of croton increase through hybridization. The information on genetic diversity and relationship between parent and its offspring of crotons is very limited. This study aims to analyze the genetic diversity of cultivar Mawar (MW) and Walet (W) as parent compare to their offspring i.e, Black Marlet (BM), Kingkit 1 (KA), Kingkit 2 (KB), and Kamaratih (KM) using RAPD markers. This study used DNA extraction from the fresh leaf of six cultivars. The next steps were DNA quantification, primary optimization, DNA amplification with PCR, and electrophoresis. Statistical analysis was carried out using Genalex software. A total of 40 primers were screened, out of which 10 were selected for the analysis of genetic diversity. A total of 106 polymorphic bands were generated, ranging from 130 to 1850 bp. The results of RAPD analysis showed that Mawar as female parent had the highest polymorphic bands percentage of 69.01%, while Walet as male parent and its offspring ranged from 31.15 % to 43.94%. The genetic distance of the offspring with Walet ranged from 0.176 to 0.234 and genetic distance of the offspring with Mawar ranged from 0.314 to 0.372. It was indicated that all of offspring were closer to the male parent.
Similarity level of Nepenthes spp. based on the qualitative characters Putri Lukmanasari; Aziz Purwantoro; Rudi Hari Murti; Zulkifli Zulkifli
Jurnal Ilmu Pertanian Vol 5, No 3 (2020): December
Publisher : Faculty of Agriculture, Universitas Gadjah Mada jointly with PISPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ipas.55728

Abstract

Nepenthes, also known as pitcher plant, are unique and interesting flora developed as ornamental plants. This species has an attraction not only on the flowers but also on the diverse shapes and colors of the pitchers. Each Nepenthes species and hybrid has a variety of characters. The genetic relationship and diversity in Nepenthes species and hybrids can be determined based on the morphological characterization. The purpose of this study was to determine the similarity level among the Nepenthes accessions based on 31 qualitative characters. In this study, 30 Nepenthes species and 11 hybrids were used. The range of the similarity coefficient of Nepenthes species was 0.28-0.65, and divided into two major groups namely group A and B. Group A was the Nepenthes population which had petioles consisted of 17 accessions, while group B had folius consisting of 24 accessions. It indicated that when the similarity value is higher, the similarity level between each plant becomes higher. Principle Coordinates Analysis (PCoA) provided an overview of the relationship among the genotypes in coordinates. Species with a high similarity coefficient were in a close position, whereas species with a low similarity coefficient were in a remote position. Based on the PCoA, the species were grouped based on the similar characters, which was supported by the dendrograms.
Somaclonal Variation of Lily And Their Chromosomes Stability Aziz Purwantoro
Jurnal Ilmu Pertanian Vol 9, No 2 (2002): November
Publisher : Faculty of Agriculture, Universitas Gadjah Mada jointly with PISPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ipas.58603

Abstract

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Analisis Diallel untuk Daya Gabung Tanaman Jagung (Zea mays L.) Pada Tiga Tingkat Kerapatan Tanam Aziz Purwantoro; Woerjono Mangoendidjojo; Lilik Kusdiarti
Jurnal Ilmu Pertanian Vol 4, No 6 (1991): Juli
Publisher : Faculty of Agriculture, Universitas Gadjah Mada jointly with PISPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ipas.58701

Abstract

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Co-Authors , Parjanto A. Widiyatmoko Achmad Syarif Sirojuddin Agus Budi Setiawan Agus Budi Setiawan, Agus Budi Agus Slamet Ainun Nikmati Laily Allaam, Daffa Ramzy Syah Aman Suyadi Andin Puspita Andin Puspita Ari Indrianto Ari Indrianto Ari Indrianto Aries Bagus Sasongko Arman Wijonarko Asadi Abdullah Asadi Asadi Asep Rinal Supratman Asri Fajar Milasari Aurellia Tatipata Bambang Sutaryo Bekti Sulistya Utami BUDI SETIADI DARYONO Christina Astri Wirasti Cristina Astri Wirasti Danarsi Diptaningsari Della Rosiana Ningtias Didik Indradewa Dinda Dewanti Djoko Prajitno Dyah Weny Respatie Edhi Martono Endang Pudjihartati Endang Semiarti Endang Sulistyaningsih Erlina Ambarwati Exsyupransia Mursyanti Exsyupransia Mursyanti Fajar Hayuatmaja Falah Nur Alifianto Fandi Setiawan Fitria Setyaningsih Gildantia, Elke Ginting, Tesalonika Gretaryan Wahyu Widiatmiko Handayani, Niken S. N. Hanifa, Yumna Hanifa, Yumna Rahmadias Harkingto Harkingto Herni Shintiavira I. Indriyati Wibisono Ilmiah, Haviah Hafidhotul Inayah, Suwaibah Ummul Irwan Gery Renaldi Ixora Sartika Mercuriani JAKA WIDADA Jalil, Muhamad Jamhari Jamhari, Jamhari Kana Ninomiya Kurniawan, Febri Yuda Lilik Kusdiarti Lita Rahmadani Maria Marina Herawati Miranda Ferwita Sari Monika Andreastuti Kusumaningrum Mufit Daryatun Asniawati Muhammad Dylan Lawrie Muhammad Dylan Lawrie Nasrullah Nasrullah Nintya Setiari Nintya Setiari Nurul Annisa Oktaviana Herawati Panjisakti Basunanda Parjanto Parjanto Pauline Destinugrainy Kasi Prapto Yudono Purnomo Purnomo Purnomo Puspita, Andin Putri Lukmanasari Putri, Aidha Rusita Qurrotul Uyun, Qurrotul Rahayu Sulistianingsih Rani Agustina Wulandari Ratih Hartono Putri Rima Indhirawati Rina Pratiwi Pudja I. A Rohimah Handayani Sri Lestari Rudi Hari Murti Rudi Hari Murti Rudi Hari Murti Sahiral Yakub SATRIYAS ILYAS Seonghoe Jang Shogo Matsumoto Sri Nopitasari Sri Trisnowati Sukarti Moeljopawiro Sukarti Moeljopawiro Sukarti Moeljopawiro Sukarti Moeljopawiro Sukarti Moeljopawiro Sukarti Moeljopawiro Sulastri Isminingsih Supriyanta Supriyanta Supriyanta Supriyanta Supriyanta Supriyanta Suwaibah Ummul Inayah SUWIJIYO PRAMONO Suwijiyo Pramono Swandari, Tantri Tahtihal Anhar Tamam, M. Badrut Tio Eka Sinurat Triono Bagus Saputro, Triono Bagus Trisnaningtyas, Carenina Valentina Dwi Suci Handayani W. T. Artama W.T. Artama Wahyu Listyaningrum Wili Setiyoko Windi Mose Woerjono Mangoendidjojo Woerjono Mangoendidjojo Y. Andi Trisyono Y. Andi Trisyono Yasushi Yoshioka Yefta Pamandungan Yeni Fatmawati Yuli Setiawati Yundari, Yundari Yuuki Asano Zulfa Layina Zulkifli Zulkifli