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All Journal HAYATI Journal of Biosciences Vegetalika Jurnal Ilmu Pertanian EUGENIA Zuriat Jurnal Ilmiah Aplikasi Isotop Radiasi BIOTROPIA - The Southeast Asian Journal of Tropical Biology Indonesian Journal of Agricultural Science AGRIVITA, Journal of Agricultural Science Jurnal Agroekoteknologi Indonesian Journal of Biotechnology Jurnal Perlindungan Tanaman Indonesia Journal of Tropical Biodiversity and Biotechnology Jurnal Bioteknologi & Biosains Indonesia (JBBI) Jurnal Pertanian Agros jurnal Ilmu Pertanian (Agricultural Science) Sains dan Matematika Agroista: Jurnal Agroteknologi BIO-SAINS | Jurnal Ilmiah Biologi
Aziz Purwantoro
Universitas Gadjah Mada
Agriculture, Biological Sciences & Forestry Astronomy Biochemistry, Genetics & Molecular Biology Chemistry Decision Sciences, Operations Research & Management Earth & Planetary Sciences Education Environmental Science Immunology & microbiology Materials Science & Nanotechnology Mathematics Physics Veterinary

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Kariotipe Kromosom Salak Parjanto Parjanto; Sukarti Moeljopawiro; W.T. Artama; Aziz Purwantoro
Jurnal Ilmu Pertanian Vol 10, No 1 (2003): Juni
Publisher : Faculty of Agriculture, Universitas Gadjah Mada jointly with PISPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ipas.60067

Abstract

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Transformation of Zinnia elegans Jacq. as an ornamental potted plant by daminozide application Nurul Annisa; Aziz Purwantoro; Dyah Weny Respatie
Jurnal Ilmu Pertanian Vol 6, No 3 (2021): December
Publisher : Faculty of Agriculture, Universitas Gadjah Mada jointly with PISPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ipas.62668

Abstract

Zinnia elegans Jacq. is one of the ornamental plants potential to be used as a potted ornamental plants. The problem to be resolved is the size of the plant can reach 1 m, so it is necessary to modify the plant height into 20 cm to 25 cm using retardant (plant growth regulator), called daminozide. The purpose of this study was to determine the best concentration and soaking time using daminozide to inhibit the growth of zinnia. The research was conducted at Mangkuyudan 57, Yogyakarta. This research was arranged in a factorial design with 3 blocks as replication. Different concentrations of daminozide were used as first factor with three levels (1 g.L-1, 2 g.L-1, and 3 g.L-1) and soaking times were used as the second factor (12 h, 24 h, and 36 h). The data were analyzed using analysis of variance and continued with HSD-Tukey at the α = 5 %. The results showed that there were an interaction between daminozide concentration and soaking time in the height of Z. elegans. There were also positive correlation between plant height, number of flower, and flowering period. The best combination of daminozide concentration and soaking times were 2 g.L-1 and 12 h. This treatment gave the best height that fit to the criteria of a potted plant which was 20.08 cm. However, it reduced flower’s diameter, number of flowers, and canopy’s size.
Effects of paclobutrazol concentrations and watering frequencies on the flowering ratio of cucumber plants (Cucumis sativus L.) Ratih Hartono Putri; Aziz Purwantoro; Valentina Dwi Suci Handayani; Dyah Weny Respatie
Jurnal Ilmu Pertanian Vol 7, No 1 (2022): April
Publisher : Faculty of Agriculture, Universitas Gadjah Mada jointly with PISPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ipas.66002

Abstract

Generally, cucumber plants have more male flowers than female flowers. The fewer number of female flowers is one of the problems of cucumber production. Flowering regulation can be performed by applying growth regulators and water management. The study aims to determine the effects of paclobutrazol concentrations and watering frequencies on the flowering ratio of cucumber plants. The experiment was arranged in a factorial Randomized Completely Block Design at the Agricultural, Food, and Fishery Extension Center, Seyegan, Yogyakarta. The first factor was the paclobutrazol concentrations consisting of four levels (a control, 0.187 mL.L-1, 0.375 mL.L-1, and 0.562 mL.L-1). Meanwhile, the second factor was the watering frequency consisting of three levels (once a day, once every two days, and once every three days). The observed data were performed using the analysis of variance (ANOVA) and followed with Duncan’s Multiple Range Test at α= 5 %. The paclobutrazol concentrations resulted in a significant effect on all of the observed variables. Meanwhile, watering frequencies showed no significant effect on the male flowers, female flowers and ratio of male to female flowers in cucumber. There was an interaction effect of paclobutrazol concentration and watering frequency on the plant height and flowering age of cucumber plants. The treatment of paclobutrazol at a concentration of 0.375 mL.L-1, decreased of male flowers and increased of female flowers, reducing the ratio of male and female flowers of cucumber plants at various watering frequencies.-1
Evaluasi Resistensi terhadap Metoksifenozida pada Spodoptera exigua di Jawa I. Indriyati Wibisono; Y. Andi Trisyono; Edhi Martono; Aziz Purwantoro
Jurnal Perlindungan Tanaman Indonesia Vol 13, No 2 (2007)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.11859

Abstract

The objective of this research was to know the resistance level of beet armyworm Spodoptera exigua to methoxyfenozide. The lara were collected from the shallot production areas in the districts of Nganjuk (East Java), Bantul and Kulonprogo (Yogyakarta Specia Territory) and Brebes (Central JAva). The bioassay used first instars of S. exigua with artificial diets that had been dipped in a series of methoxyfenozide solutions. Larval mortality was recorded at 72 hours after the exposure. The LC50 value of methoxyfenozide for the population from Sanden (Bantul) was the lowest (0.53 ppm) among 16 tested population, therefore it was used as refenrence population to determine the resistance ratio (RR) values of other populations. The RR values of the tested populations varied from 1 to 240,8 times. Population collected from Wanasari (Brebes) was found to have the highest level of resistance of methoxyfenozide as a result of using methoxyfenozide intensively to S. exigua controlling.
Pewarisan Sifat Resistensi terhadap Deltametrin pada Plutella xylostella Wahyu Listyaningrum; Y. Andi Trisyono; Aziz Purwantoro
Jurnal Perlindungan Tanaman Indonesia Vol 9, No 1 (2003)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.12284

Abstract

A field population of Plutella xylostella (Lepidoptera: Plutellidae) in Central Java has been reported to become resistant to a pyretroid synthetic deltamethrin. The objective of this research was to study the genetics of resistance in that population. The resistant population wascontinously sprayed with deltamethrin in the laboratory to increase the resistant level. The resistant population used in this study was 51 more resistant to deltamethrin than was the susceptible population. Bioassays using F1 obtaining from the reciprocal mating between the susceptible and resistant parents showed that the resistance to deltamethrin in P. xylostella was inherited recessively and the maternal effects were observed. The degree of dominance (D) was -0.8 when resistant female was mated with the susceptible male and the D value was -0.3 when the susceptible female was mated with the resistant male. Using the monogenic model it was proved that the resistance was controlled by single gen. The implementation of these findings for the development of resistance management program will be discussed.
Stability of Resistance to Imidacloprid in the Brown Planthopper (Nilaparvata lugens Stål.) from Banyumas, Central Java Danarsi Diptaningsari; Y. Andi Trisyono; Aziz Purwantoro; Arman Wijonarko
Jurnal Perlindungan Tanaman Indonesia Vol 24, No 1 (2020)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.43954

Abstract

Imidacloprid is one of the insecticides that has been widely used to control the brown planthopper (Nilaparvata lugens Stål.). The excessive use of insecticides led to the development of insecticide resistance in N. lugens. This study was aimed to determine the stability of resistance to imidacloprid in N. lugens from Banyumas, Central Java Province. The five generations of selection increased the resistance ratio (RR) from 46.20-fold to 150.39-fold. To assess the stability of resistance to imidacloprid in N. lugens, the fifth generation was further reared for 10 generations without exposure to insecticides. The stability studies showed that resistance to imidacloprid was unstable with a decrease of resistance from 150.39-fold to 38.14-fold after 10 generations without selections. Continuous selections with imidacloprid for three generations could increase the resistance level from 150.39-fold to 216.13-fold. The unstable resistance could be managed by removing the selection pressure for a period of time or switching to insecticides with different mode of actions. Information regarding resistance stability would be useful to determine an effective resistance management strategies in N. lugens.
Micropropagation of Mini Orchid Hybrid Phalaenopsis “Sogo Vivien” Exsyupransia Mursyanti; Aziz Purwantoro; Sukarti Moeljopawiro; Endang Semiarti
Journal of Tropical Biodiversity and Biotechnology Vol 1, No 1 (2016): June
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (426.637 KB) | DOI: 10.22146/jtbb.12933

Abstract

Phalaenopsis “Sogo Vivien” is an orchid hybrid with mini size plant body, and exhibits numerous beautiful pink flowers, that is ideal as ornamental pot plant. Some plants of this orchid exhibit variegated leaves that improve the beauty of the plant, not only because of the flower but also as attracted leaves. This orchid has high economical value, but mass propagation of this orchid has not established yet. An effective method to propagate both the normal and variegated plants is worth to be generated. The objective of this research was to produce a large number of P. “Sogo Vivien” plants, including the variegated plants. The method used seeds from self pollinating variegated plant, and flower stalk nodes. The seeds were sown on three various medium: VW, NP and MS, and flower stalk nodes were planted on VW + BA 10 mg l-1 + active carbon. The results showed that the best medium for in vitro culture of P. “Sogo Vivien” was NP medium, in which all seeds could grew into plantlets. Most plantlets emerged from the seeds were non variegated, only one plantlet out of 1344 seeds was variegated (0.007%). Although all emerged plantlets from flower stalk exhibited variegated leaves. Particularly, the plantlets arised from the second and third basal nodes of flower stalk showed the highest growth rate than that from the other nodes. Histological analysis showed that at 11-13 days after shoot segment plantation on NP medium, the shape of apical cells in the nodes was changed, then followed by the change of cell shape in the basal part of the nodes, produced bipolar pattern, then gradually developed into shoot. These results suggest that mass propagation could be achieved using seed culture, but to get the variegated phenotypes, the second and third nodes of flower stalk from variegated plant were the best explants to be used.
Peptone and tomato extract induced early stage of embryo development of Dendrobium phalaenopsis Orchid Nintya Setiari; Aziz Purwantoro; Sukarti Moeljopawiro; Endang Semiarti
Journal of Tropical Biodiversity and Biotechnology Vol 1, No 2 (2016): December
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (3370.3 KB) | DOI: 10.22146/jtbb.15498

Abstract

Germination and growth of orchid seeds can be accelerated by the addition of organic supplement and plant extract in culture medium. The objective of this study was to determine the effect of peptone and tomato extract on early stage of embryo development of Dendrobium phalaenopsis orchids. Orchid seeds were sown on NP and VW medium with addition of 10% of CW (NPCW and VWCW).  Five weeks after seed germination, about 58.03% seed germination was observed on VWCW medium, and only 37.45% seed germination on NPCW. Tomato extract and peptone were added in VWCW, resulting VWCWTP medium. After 4-8 weeks on VWCWTP, 94.42% seeds was germinated into plantlet, but only 67.30% germinated seeds on VWCW. To get optimal growth and development of  D.  phalaenopsis orchids embryos in the in vitro condition, supplement of 100 ml.L-1 coconut water, 100 mg.L-1 tomato extract and 2 mg.L-1 peptone into VW basic medium is required.
Innovation of Natural Orchid Cultivation Technology for Tourism Development in Banyunganti Hamlet, Jatimulyo Village, Girimulyo Sub-District, Kulon Progo District, Yogyakarta Endang Semiarti; Aziz Purwantoro; Ari Indrianto; Aries Bagus Sasongko; Oktaviana Herawati; Asri Fajar Milasari
Journal of Tropical Biodiversity and Biotechnology Vol 5, No 3 (2020): December
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jtbb.46283

Abstract

Orchid is the best tourism icon which focused on nature-based tourism development in Indonesia. Banyunganti Hamlet is one of the tourism villages in Kulon Progo which has a high diversity of orchid species. Regarding this situation, guiding and assistance for villagers which is focused on the introduction of natural orchid species and its character, conventional propagation, and modern propagation by using household-scale tissue culture techniques (sowing seeds and planting) is important to give. The long-term goal for this activity was for empowering women in Dusun Banyunganti in line with the opening of the New Yogyakarta International Airport by the government.
PHENOTYPIC AND MOLECULAR CHARACTERIZATION OF MULTISHOOTS DEVELOPMENT IN TRANSGENIC Phalaenopsis amabilis (L.) BLUME HARBORING 35S::KNAT1 (KNOTTED-LIKE Arabidopsis thaliana 1) Triono Bagus Saputro; Endang Semiarti; Aziz Purwantoro
BIOTROPIA - The Southeast Asian Journal of Tropical Biology Vol. 25 No. 1 (2018)
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11598/btb.2018.25.1.615

Abstract

Phalaenopsis amabilis (L.) Blume is one of Indonesian natural orchid which has an aesthetic flower and possesses high economic value. The low multiplication rate and long periods of life cycle are the main obstacles to conventionally propagate this orchid. The aims of this research were to analyze the stability of transgenic plant P. amabilis harboring 35S::KNAT1 based on morpho-genomic characterization. KNAT1 gene is reported as a gene that involved in the shoot formation, and it  had been successfully introduced into Phalaenopsis amabilis (L.) Blume genome. After seven times regeneration, the confirmation of the transgene existence in the genom is needed to ensure whether the plant could consistently maintain the transgene in its genome and to characterize the shoot development. The experiment was carried out in 3 steps:  1) Co-integration analysis of 35S::KNAT1 into P. amabilis genom; 2) Phenotypic analysis on the multiplication rate, morphological variation and venation pattern; and 3) Protein profile analysis of transgenic plants. The results showed that the survival rate of putative transgenic was 58.7% on NP0 medium and 62.5% on NP SIM medium. PCR analysis confirmed that 82.5% transgenic growth on NP0 and 93.33% on NP SIM contained DNA fragment of KNAT1 gene, NPTII gene and trnL-F intergenic spacer, indicating that those plants are positive transgenic. The 35S::KNAT1 transgenes and phytohormone were independently involved in multishoots formation of P. amabilis transgenic plants. The phenotypic of plantlets were classified into six main criteria, i.e. normal shape, lobed leaves, rosette, elongated stem, cup shoot and widened leaves. The normal type was the most abundant type of variation (± 29%) in both medium. Protein profile showed that all transgenic plants produced 45,8 kDa protein and that was equivalent with molecular weight of KNAT1 protein. Taken together, all those data indicated that 35S::KNAT1 transgene were consistently integrated into the transgenic plant genome.
Co-Authors , Parjanto A. Widiyatmoko Achmad Syarif Sirojuddin Agus Budi Setiawan Agus Budi Setiawan, Agus Budi Agus Slamet Ainun Nikmati Laily Allaam, Daffa Ramzy Syah Aman Suyadi Andin Puspita Andin Puspita Ari Indrianto Ari Indrianto Ari Indrianto Aries Bagus Sasongko Arman Wijonarko Asadi Abdullah Asadi Asadi Asep Rinal Supratman Asri Fajar Milasari Aurellia Tatipata Bambang Sutaryo Bekti Sulistya Utami BUDI SETIADI DARYONO Carenina Trisnaningtyas Christina Astri Wirasti Cristina Astri Wirasti Danarsi Diptaningsari Della Rosiana Ningtias Didik Indradewa Dinda Dewanti Djoko Prajitno Dyah Weny Respatie Edhi Martono Endang Pudjihartati Endang Semiarti Endang Sulistyaningsih Erlina Ambarwati Exsyupransia Mursyanti Exsyupransia Mursyanti Fajar Hayuatmaja Falah Nur Alifianto Fandi Setiawan Fitria Setyaningsih Gildantia, Elke Ginting, Tesalonika Gretaryan Wahyu Widiatmiko Handayani, Niken S. N. Hanifa, Yumna Rahmadias Harkingto Harkingto Herni Shintiavira I. Indriyati Wibisono Irwan Gery Renaldi Ixora Sartika Mercuriani JAKA WIDADA Jamhari Jamhari, Jamhari Kana Ninomiya Lilik Kusdiarti Lita Rahmadani Maria Marina Herawati Miranda Ferwita Sari Monika Andreastuti Kusumaningrum Mufit Daryatun Asniawati Muhammad Dylan Lawrie Muhammad Dylan Lawrie Nasrullah Nasrullah Nintya Setiari Nintya Setiari Nurul Annisa Oktaviana Herawati Panjisakti Basunanda Parjanto Parjanto Pauline Destinugrainy Kasi Prapto Yudono Purnomo Purnomo Puspita, Andin Putri Lukmanasari Putri, Aidha Rusita Rahayu Sulistianingsih Rani Agustina Wulandari Ratih Hartono Putri Rima Indhirawati Rina Pratiwi Pudja I. A Rohimah Handayani Sri Lestari Rudi Hari Murti Rudi Hari Murti Rudi Hari Murti Sahiral Yakub SATRIYAS ILYAS Seonghoe Jang Shogo Matsumoto Sri Nopitasari Sri Trisnowati Sukarti Moeljopawiro Sukarti Moeljopawiro Sukarti Moeljopawiro Sukarti Moeljopawiro Sukarti Moeljopawiro Sukarti Moeljopawiro Sulastri Isminingsih Supriyanta Supriyanta Supriyanta Supriyanta Suwaibah Ummul Inayah Suwijiyo Pramono SUWIJIYO PRAMONO Swandari, Tantri Tahtihal Anhar Tamam, M. Badrut Tio Eka Sinurat Triono Bagus Saputro, Triono Bagus Valentina Dwi Suci Handayani W. T. Artama W.T. Artama Wahyu Listyaningrum Wili Setiyoko Windi Mose Woerjono Mangoendidjojo Woerjono Mangoendidjojo Y. Andi Trisyono Y. Andi Trisyono Yasushi Yoshioka Yefta Pamandungan Yeni Fatmawati Yuli Setiawati Yumna Hanifa Yundari, Yundari Yuuki Asano Zulfa Layina Zulkifli Zulkifli