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PENINGKATAN KESADARAN WARGA RT 014/RW 008 JATIPULO, JAKARTA BARAT MENGENAI KESEHATAN GIGI PADA MASA PANDEMI COVID-19 Sheila Soesanto; Octarina Octarina; Joko Kusnoto
Jurnal AKAL : Abdimas dan Kearifan Lokal Vol. 2 No. 1 (2021): Jurnal AKAL : Abdimas dan Kearifan Lokal
Publisher : Universitas Trisakti

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (2388.965 KB) | DOI: 10.25105/akal.v2i1.9032

Abstract

Selama masa pandemi Covid-19 ini, pelayanan bidang kesehatan, khususnya bidang kedokteran gigi hanya menangani kasus-kasus emergensi. Pembatasan ini dilakukan karena resiko penularan virus antara dokter gigi dan pasien sangatlah tinggi mengingat posisi dokter gigi dan pasien ‘face to face’ selama perawatan. Komunitas masyarakat di RT 014/RW 008, Kelurahan Jatipulo, Kecamatan Palmerah, Jakarta Barat merupakan daerah yang padat penduduk dalam lingkup yang sempit dan sederhana. Oleh karena itu, sangat diperlukan edukasi pada masyarakat untuk meningkatkan kebersihan gigi dan mulut secara khusus agar terhindar dari gigi berlubang dan mengetahui cara pembersihan gigi yang tepat. Dengan demikian masyarakat dapat menghindari pergi ke ke dokter gigi yang merupakan upaya pencegahan dari terjangkitnya virus Covid-19. Penyuluhan kesehatan gigi dilakukan pada komunitas masyarakat di RT 014/RW 008, melalui media Zoom pada tanggal 28 Juli 2020. Materi yang diberikan meliputi penjelasan mengenai pentingnya menjaga kesehatan gigi dan mulut, demonstrasi menyikat gigi yang baik, pelatihan sikat gigi bersama, dan sesi tanya jawab. Penyuluhan berjalan dengan lancar yang ditandai dengan adanya komunikasi dua arah dengan masyarakat. Selain itu, terlihat antusias warga yang tinggi waktu menyikat gigi bersama dan berkonsultasi mengenai masalah-masalah kesehatan gigi yang mereka alami. Dilakukan pemberian kuesioner sebelum dan sesudah penyuluhan yang meliputi pengetahuan seputar kesehatan gigi. Pengetahuan komunitas masyarakat di RT 14/RW 008, Jatipulo mengenai cara menyikat gigi yang baik dan benarpun bertambah dengan pemberian materi penyuluhan. Hal ini terlihat dari hasil penilaian kuesioner yang meningkat setelah dilakukan penyuluhan.
PENILAIAN HASIL PENYULUHAN PROTOKOL KESEHATAN GIGI DAN MULUT SELAMA MASA PEMBELAJARAN JARAK JAUH (KAJIAN PADA SISWA SMPN 1 DRAMAGA, BOGOR JAWA BARAT) Komariah Komariah; Octarina Octarina; Lies Zubardiah; Del Afriadi Bustam
Jurnal AKAL : Abdimas dan Kearifan Lokal Vol. 2 No. 2 (2021): Jurnal AKAL : Abdimas dan Kearifan Lokal
Publisher : Universitas Trisakti

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (2858.64 KB) | DOI: 10.25105/akal.v2i2.10267

Abstract

Pandemi Covid-19 yang telah berjalan selama setahun, sangat mengganggu disegala bidang tanpa kecuali dibidang pendidikan. Penutupan sekolah SMPN 1 Dramaga, satu langkah yang dilakukan pemerintah guna menurunkan rantai penularan dengan melakukan aktivitas sekolah di rumah, dengan mengubah sistem pembelajaran dari pembelajaran tatap muka (PTM) menjadi pembelajaran jarak jauh (PJJ). Keterbatasan pengetahuan siswa mengenai Covid-19, protokol kesehatan, serta kesehatan gigi dan mulut menjadi kendala pada masa pandemi pada kesehatan siswa. Penyuluhan dilakukan untuk mengetahui kendala yang dihadapi siswa SMPN 1 Dramaga selama masa PJJ, pengetahuan kesehatan gigi dan mulut, serta Prokes selama pandemi Covid-19. Hasil dari kuesioner yang diberikan memperlihatkan pada semua point kuesioner terjadi peningkatan baik pada pengetahuan protokol kesehatan maupun pengetahuan tentang kesehatan gigi dan mulut.
Perbandingan Kekuatan Tekan Gipsum Bangunan, Dental Plaster, Dan Orthodontic Plaster Nadya Putri Winandari; Octarina Octarina; Johan Arief Budiman
Jurnal Kedokteran Gigi Terpadu Vol. 2 No. 1 (2020): Jurnal Kedokteran Gigi Terpadu
Publisher : Fakultas Kedokteran Gigi Universitas Trisakti

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1105.584 KB) | DOI: 10.25105/jkgt.v2i1.7513

Abstract

Latar Belakang: Gipsum merupakan mineral alam yang dimanfaatkan dalam konstruksi bangunan dan bidang kedokteran gigi. Bahan dasar partikel -hemihidrat dimiliki oleh gipsum bangunan serta gipsum kedokteran gigi tipe 2 yaitu dental plaster dan orthodontic plaster. Sifat mekanis berupa kekuatan tekan memiliki peranan penting dalam aplikasi gipsum di bidang kedokteran gigi. Tujuan: Untuk membandingkan kekuatan tekan gipsum bangunan dan gipsum kedokteran gigi tipe 2 setelah 24 jam. Metode: Penelitian eksperimental laboratoris dibagi kedalam 3 kelompok (gipsum bangunan, dental plaster, orthodontic plaster) yang masing-masing terdiri dari 10 sampel. Gipsum dimanipulasi dengan perbandingan 50 g bubuk dan 25 mL akuades, diaduk hingga homogen menggunakan gypsum mixer (HL-YMC, Cina). Adonan gipsum dituang pada mould silindris berukuran (20±0,2) mm x (40±0,4) mm sesuai standar ISO 6873/ Spesifikasi ADA No. 25 dan diletakkan pada vibrator. Berat gipsum ditimbang setelah pembuatan sampel dan setelah 24 jam. Pengujian kekuatan tekan dilakukan 24 jam setelah pembuatan sampel menggunakan Universal Testing Machine (Shimadzu AGX-V 10kN, Japan) dengan chs 1 mm/min. Hasil: Pada penelitian didapatkan perbandingan nilai rerata kekuatan tekan gipsum bangunan (11,51 0,65) MPa, dental plaster (11,18 0,75) MPa, dan orthodontic plaster (13,15 0,79) MPa. Orthodontic plaster memiliki kekuatan tekan tertinggi sedangkan nilai kekuatan tekan gipsum bangunan tidak berbeda bermakna dengan dental plaster. Analisis menggunakan One way ANOVA dan Post Hoc-Tukey menjelaskan bahwa nilai kekuatan tekan antar kelompok memiliki perbedaan signifikan dengan p<0,05. Perbedaan bermakna terjadi antara kelompok orthodontic plaster terhadap gipsum bangunan dan dental plaster. Kesimpulan: Penelitian ini menunjukkan bahwa gipsum bangunan dapat digunakan sebagai bahan alternatif gipsum kedokteran gigi tipe 2.
Pengaruh aplikasi bovine amniotic membrane pada soket tulang alveolar terhadap ekspresi BMP-2: studi eksperimental in vitro Geneva Ariesta; Octarina Octarina; Elly Munadziroh; Ekowati Handharyani
Jurnal Kedokteran Gigi Universitas Padjadjaran Vol 35, No 2 (2023): Agustus 2023
Publisher : Fakultas Kedokteran Gigi Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/jkg.v35i2.46718

Abstract

ABSTRAKPendahuluan: Pencabutan gigi dapat menyebabkan resorpsi tulang alveolar. Bovine Amniotic Membrane (BAM) diharapkan menjadi material pengisi soket tulang alveolar sehingga dapat mengurangi terjadinya resorpsi tulang alveolar setelah dilakukan pencabutan. BMP-2 merupakan indikator terjadinya pembentukan tulang alveolar. Tujuan penelitian ini adalah menganalisis ekspresi BMP-2 dengan aplikasi BAM pada soket tulang alveolar tikus Sprague dawley (SD). Metode: …….. …….. Jenis penelitan ini adalah eksperemintal laboratorik. Teknik pengambilan jumlah sampel menggunakan rumus Federer. Sebanyak 16 tikus Sprague dawley jantan dibagi menjadi dua kelompok, yaitu kelompok kontrol dan kelompok BAM. Pencabutan dilakukan pada gigi insisivus sentral kanan mandibula. Setelah dilakukan pencabutan, pada kelompok bam soket alveolar diaplikasikan material BAM dengan ukuran 1,5x10 mm. Hari ke-14 dan ke-28, euthanasia dilakukan, kemudian dilakukan pemotongan pada bagian soket tulang alveolar yang akan dipreparasi menjadi preparat imunohistokimia (IHK). Pembuatan preparat IHK menggunakan pewarnaan antigen-antibodi BMP-2 (1:200, Affinity Biosciences, Inc. USA). Analisis preparat IHK menggunakan mikroskop ZEISS seri AXIO SCOPE A.1 dan software ZEN 3.4 perbesaran 40x. Perhitungan ekspresi BMP-2 menggunakan software imageJ. Analisis data statistik penelitian menggunakan one-way ANOVA dengan uji post hoc Tukey. Hasil:  Nilai rerata ekspresi BMP-2 pada kelompok kontrol hari ke-14 dan ke 28 secara berurutan (5,93 ± 0,70) dan (6,60 ± 1,11). Kelompok BAM menunjukkan adanya peningkatan nilai rerata ekspresi BMP-2 pada hari ke-14 dan ke-28 secara berurutan (9,46 ± 0,50) dan (11,73 ± 3,60) secara statistik dengan one way annova pos hoc tukey berbeda bermakna (p<0,05). Simpulan: Aplikasi material BAM pada soket tulang alveolar dapat meningkatkan ekspresi BMP-2 pada hari ke-14 dan 28. Hal tersebut akan menyebabkan penyembuhan tulang alveolar dan berkurangnya resorpsi tulang alveolar.The effect of bovine amniotic membrane application at the alveolar bone socket on the expression of BMP-2: in vitro experimental studyABSTRACTIntroduction: Tooth extraction can cause alveolar bone resorption. Bovine Amniotic Membrane (BAM) is expected to be a filling material for the alveolar bone socket so that it can reduce the occurrence of alveolar bone resorption after extraction. BMP-2 is an indicator of alveolar bone formation. The application of BAM is expected to reduce of alveolar bone resorption after extraction. Aim: The aim of this study was to analyze the expression of BMP-2 by application of BAM in the alveolar bone socket of Sprague Dawley rats (SD). Methods: This type of research is laboratory experimental. The sampling technique used the Federer formula. A total of 16 male SD rats were divided into two groups, which are the control group and the BAM group. Extraction was performed on the mandibular right central incisor. In the BAM group, after removal of the alveolar socket BAM material with a size of 1.5 x 10 mm was applied. On the 14th and 28th day, mice were euthanized and then cut into the alveolar bone socket which will be prepped into an immunohistochemical specimen (IHK). Preparation of IHK will be prepped using BMP-2 antigen-antibody staining (1:200, Affinity Biosciences, Inc. USA). Analysis of CPI preparate using the ZEISS AXIO SCOPE A.1 series microscope and ZEN 3.4 software with 40x magnification. BMP-2 expression calculation using imageJ software. Statistical data analysis of the research used one-way ANOVA with Tukey's post hoc test. Results: The mean value of BMP-2 expression in the control group on day 14 and 28 respectively (5.93 ± 0.70) and (6.60 ± 1.11). The BAM group showed an increase in the mean value of BMP-2 expression on the 14th and 28th days respectively (9.46 ± 0.50) and (11.73 ± 3.60) which were statistically significantly different (p<0, 05). Conclusion: Application of BAM to the alveolar bone socket can increase BMP-2 expression on days 14 and 28. This will lead to alveolar bone healing and reduced alveolar bone resorption.Key wordsbovine amnion membrane, bone morphogenetic protein 2, alveolar socket preservation
Pengaruh suhu air terhadap setting time dari bahan cetak alginat Tansza S. Putri; Deviyanti Pratiwi; Eddy; Rosalina Tjandrawinata; Dewi L. Margaretta; Florencia L. Kurniawan; Octarina
e-GiGi Vol. 12 No. 1 (2024): e-GiGi
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35790/eg.v12i1.47105

Abstract

Abstract: Irreversible hydrocolloid impression materials or alginate is commonly used in dental practice. Its setting time range is 1–4.5 minutes. The setting time is affected by several factors such as water temperature mixed with the alginate powder. There are previous studies evaluated the effect of water temperature on the alginate’s setting time, however, the collected data is still minimal. Therefore, this study aimed to evaluate the effect of water temperature on alginate’s setting time on larger scale. There were total of 423 samples divided into three groups: cold, moderate, and warm temperature of water mixed with alginate; each group consisted of 141 samples. Differences in setting times of the qroups were analyzed with the Kruskal-Wallis test and the Tukey’s HSD. Alginate powder used in this study was the normal-set type. Mixing the alginate powder with water was performed as the factory instruction, and then the mixture was poured into molds and the setting times were measured. The results showed that the setting times of the three groups were significantly different. Group I (cold) had the highest setting time (211 seconds), followed by Group II (room temperature) which was 147 seconds, and Group III (warm) had a setting time of 106 seconds.  In conclusion, water temperature has an effect on setting time, that is, the higher the temperature, the faster the setting time, and vice versa. Keywords: alginate; irreversible hydrocolloid; setting time; water temperature   Abstrak: Bahan cetak hidrokoloid ireversibel atau alginat merupakan bahan umum yang digunakan di praktek kedokteran gigi, dan memiliki setting time antara 1–4,5 menit. Setting time ditentukan oleh beberapa faktor di antaranya suhu air yang digunakan dalam mencampur bahan cetak tersebut. Terdapat penelitian-penelitian terdahulu yang mengevaluasi efek suhu terhadap setting time, namun data yang dikumpulkan masih minimal. Penelitian ini bertujuan untuk mengetahui efek suhu terhadap setting time bahan cetak alginat dalam skala besar. Terdapat tiga kelompok penelitian yaitu menggunakan suhu air yang dicampurkan dengan alginat dingin, sedang (suhu ruang), dan hangat. Besar sampel penelitian ini ialah 141 sampel per kelompok, sehingga total sampel ialah 423 sampel. Perbandingan setting time dari tiga kelompok kemudian dianalisis secara statistik menggunakan uji Kruskal-Wallis dan Tukey’s HSD untuk mengetahui ada tidaknya perbedaan bermakna dari kelompok-kelompok tersebut. Bubuk alginat yang digunakan merupakan alginat tipe normal. Pencampuran bubuk alginat dengan air dilakukan sesuai dengan instruksi pabrik. Adonan yang telah diaduk dituang ke dalam cetakan dan dilakukan pengukuran setting time. Hasil penelitian mendapatkan setting time dari ketiga kelompok berbeda secara bermakna. Kelompok I (dingin) memiliki setting time tertinggi yaitu selama 211 detik, diikuti kelompok II (sedang) selama 147 detik, dan kelompok III (hangat) selama 106 detik. Simpulan penelitian ini ialah suhu air memiliki efek terhadap setting time, yaitu semakin tinggi suhu air maka semakin singkat setting time, demikian pula sebaliknya. Kata kunci: alginat; hidrokoloid ireversibel; setting time; suhu air
Peningkatan Pengetahuan Lansia Anggrek Bulan Untuk Pencegahan Kehilangan Gigi Octarina; Cindy; Nugroho, Didi; Kusnoto, Joko; Roro Asyurati Asia, Raden; Sundjoyo, Mikha
JURNAL ABDIMAS KESEHATAN TERPADU Vol. 3 No. 1 (2024): Jurnal Abdimas Kesehatan Terpadu
Publisher : Fakultas Kedokteran Gigi, Universitas Trisakti bekerjasama dengan Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Trisakti

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25105/jakt.v3i1.20122

Abstract

Dental and oral health for elderly is crucial and has impact on the individual’s general health. Physical decline in the elderly is characterized by skin starting to shrink, hair beginning to change colour, loss of bone density, ability to see up to loss of mucous teeth. Tooth loss can interfere with the function of chewing, speaking and aesthetics. This condition can be aggravated by poor dental and oral hygiene. Therefore, dental health education is important to provide elderly an information about how to maintain oral health and prevent dental problem. The method used in this research is by providing education through power point media, given to Anggrek Bulan Elderly Community in November 25th 2023. Evaluation of participants’ knowledge was carried out before and after education by giving assessment sheet in the form of multiple choices to determine the level of community knowledge. Based on evaluation results, it was found that there was an increase in knowledge among the participants. This can be seen in average value of post-test results which has increased.
Aggregatibacter actinomycetemcomitans dan Porphyromonas gingivalis: Bakteri Destruksi Tulang Alveolar Berliana, Stefhanie; Octarina
Jurnal Kedokteran Gigi Terpadu Vol. 6 No. 1 (2024): Jurnal Kedokteran Gigi Terpadu
Publisher : Fakultas Kedokteran Gigi Universitas Trisakti

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25105/jkgt.v6i1.20817

Abstract

The oral cavity is a suitable habitat for various types of bacteria, including Aggregatibacter actinomycetemcomitans and Porphyromonas gingivalis. Both of these bacteria can damage periodontal tissues, including alveolar bone, through various mechanisms. Prevention against these bacteria can be accomplished by maintaining oral hygiene. The purpose of this literature review was to provide information about bacteria that can cause damage to alveolar bone and ways to prevent it. The results of this literature review would provide information about the mechanisms of Aggregatibacter actinomycetemcomitans and Porphyromonas gingivalis bacteria in causing alveolar bone damage, as well as ways to prevent these bacteria.
Metode uji sitotoksisitas biomaterial dengan  bentuk scaffold padatan dan berpori spons Belatriks Kalangit, Ruth; Octarina
Jurnal Kedokteran Gigi Terpadu Vol. 6 No. 1 (2024): Jurnal Kedokteran Gigi Terpadu
Publisher : Fakultas Kedokteran Gigi Universitas Trisakti

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25105/jkgt.v6i1.20886

Abstract

Three-dimensional scaffolds play a crucial role in supporting cell growth and tissue regeneration, especially in bone regenerative applications. Their utilization in dentistry demands effective cytotoxicity assessment, particularly for sponge-shaped scaffolds with a unique porous structure fostering a complex environment for cell growth. Therefore, the imperative lies in selecting cytotoxicity testing methods suitable for these characteristics. Relevant methods for such scaffolds include MTS, Alamar Blue, ATP, CCK-8, and MTT assays. MTS assay efficiently measures cell viability in a three-dimensional setting, while Alamar Blue and CCK-8 assays, with their water-soluble nature and high reproducibility, prove advantageous. ATP assay provides direct insights into cellular metabolic activity, and MTT assay assesses cell viability based on enzymatic capabilities. Due to their water-soluble characteristics, minimizing additional extraction steps, and ensuring high reproducibility, Alamar Blue and CCK-8 emerge as the most fitting options for cytotoxicity assessment in dentistry's sponge-shaped scaffolds.
Antibacterial and cytotoxic effects of fresh bovine amniotic membrane with hydroxyapatite (BAM-HA): a laboratory experiment Octarina, Octarina; Berliana, Stefhanie; Kalangit, Ruth Belatriks
Padjadjaran Journal of Dentistry Vol 36, No 1 (2024): March 2024
Publisher : Faculty of Dentistry Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/pjd.vol36no1.53128

Abstract

Introduction: Bacterial infections, particularly by Aggregatibacter actinomycetemcomitans (A. actinomycetemcomitans) and Porphyromonas gingivalis (P. gingivalis), can worsen alveolar bone resorption after tooth extraction. The capability of Bovine Amniotic Membrane-Hydroxyapatite (BAM-HA) biocomposite to reduce this resorption has been explored. However, before clinical use, cytotoxicity testing is imperative to ensure its biocompatibility. The aim of the study was to assess both the antibacterial effects and cytotoxicity of the BAM-HA biocomposite to ensure its suitability for clinical use biocompatibility of the BAM-HA biocomposite before its clinical application. Methods: The laboratory-based research involved testing BAM combined with HA powder in 4:1 and 4:2 ratios via freeze-drying and underwent antibacterial tests against A. actinomycetemcomitans and P. gingivalis, using the plate count method. Cytotoxicity tests were performed on HGF cells, including negative control, positive control, BAM-HA (4:1), and BAM-HA (4:2) groups, with statistical analysis conducted using One-Way ANOVA and Post Hoc Bonferroni and Tukey tests. Results: Antibacterial tests against A. actinomycetemcomitans revealed significant reduction in colony count with BAM-HA ratios 4:1 (129.0 ± 12.7 CFU/mL) and 4:2 (77.3 ± 15.5 CFU/mL) compared to the negative control (186.6 ± 27.5 CFU/mL). Similar reductions were observed for P. gingivalis, with BAM-HA ratios 4:1 (51.3 ± 6.6 CFU/mL) and 4:2 (3.1 ± 1.5 CFU/mL) compared to the negative control (117.3 ± 22.0 CFU/mL). Cytotoxicity tests showed no significant differences in HGF cell viability and IC50 values between the negative control and BAM-HA (4:1) or BAM-HA (4:2) groups. Conclusion: The BAM - HA biocomposite shows antibacterial effects against A. actinomycetemcomitans and P. gingivalis. Moreover, BAM - HA ratios of 4:1 and 4:2 do not induce cytotoxic effects on human gingival fibroblasts, suggesting potential biocompatibility for clinical applications.KeywordA. actinomycetemcomitans, antibacterial effects, BAM-HA biocomposite, cytotoxicity, P. gingivalis
The effect of application of bovine amniotic membrane on osteoblasts, osteocytes, and collagen in the post-extraction alveolar bone socket of Sprague Dawley rats Wibowo, Artdhea Regita; Octarina, Octarina; Munadziroh, Elly; Handharyani, Ekowati
Padjadjaran Journal of Dentistry Vol 35, No 2 (2023): July 2023
Publisher : Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/pjd.vol35no2.46522

Abstract

ABSTRACTIntroduction: Tooth extraction can result in loss of alveolar bone. The application of biomaterials Bovine Amniotic Membrane (BAM) that containing growth factors and collagen is expected to play a role in socket preservation. This study aimed to observe the effect of BAM application on the growth of osteoblasts, osteocytes, and collagen in histological preparate of the alveolar bone socket of Sprague Dawley rats (SD). Methods: 16 male SD rats were used in this group. SD rats were extracted on the mandible central incisivus. Afterward, it was divided into two groups, specifically the control group (without treatment) and the treatment group (BAM application). After extraction, the treatment group was applied with BAM with a size of 1.5 mm x 10 mm in socket alveolar bone. On the 14th and 28th day, the rats were euthanized and the alveolar bone socket of mandibular tissue was taken and stained with Hematoxylin Eosin (HE) and Masson Trichrome (MT). Results: The results of all groups showed that the number of osteoblasts and collagen thickness was higher on day 14th compared to day 28th, while osteocytes continued to increase from day 14th to 28th. The thickness of collagen, osteoblasts, and osteocytes in groups with BAM application was higher than without application. Statistical results showed that there were significant differences between groups (p<0.05). Conclusion: The application of BAM can act as material preservation socket. This biomaterial can accelerate recovery by increasing collagen thickness, osteoblasts, and osteocytes.