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All Journal Indonesian Journal of Mathematics and Natural Sciences Jurnal Penelitian Pendidikan Lembaran Ilmu Kependidikan Rekayasa: Jurnal Penerapan Teknologi dan Pembelajaran Scientific Journal of Informatics Journal of Biology Education Agriprima : Journal of Applied Agricultural Sciences Unnes Science Education Journal Jurnal Gizi dan Dietetik Indonesia (Indonesian Journal of Nutrition and Dietetics) Jurnal Gizi Klinik Indonesia Biosaintifika: Journal of Biology & Biology Education Jurnal Pengabdian Magister Pendidikan IPA Jurnal Kreativitas PKM JURNAL PANGAN Journal of Innovative Science Education Berkala Penelitian Hayati Life Science Proceeding of International Conference on Science, Education, and Technology Bookchapter Alam Universitas Negeri Semarang Prosiding Seminar Nasional Biologi Life Science Journal of Creativity Student Berdaya Indonesian Journal of Community Empowerment
Siti Harnina Bintari
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Computer Science & IT Education Engineering Environmental Science Health Professions Immunology & microbiology Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Nursing Public Health Social Sciences Other

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Journal : life science

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Pengaruh Lama Penyimpanan Biji Kopi Liberika pada Suhu Kamar terhadap Mutu Mikrobiologis dan Kadar Okratoksin A: The Effect of Storage Time of Liberica Coffee Beans at Room Temperature on Microbiological Quality and Ochratoxin A Levels Ramadani, Mutiara Bintang; Dewi Mustikaningtyas; Siti Harnina Bintari; Pramesti Dewi
Life Science Vol. 14 No. 1 (2025): May 2025
Publisher : Universitas Negeri Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/unnesjlifesci.v14.i1.5810

Abstract

Liberica coffee is one of the leading commodities in several regions so it has quite high economic potential. Coffee beans can be stored for quite a long time, more than one year, even more than 4 years before going through the roasting process. However, during long storage periods coffee beans can be attacked by microorganisms such as mold (ochratoxigenic and non-ochratoxigenic). This research aims to determine the effect of storage time on the quality of Liberica coffee beans, both microbiologically and chemically. The benefit of this research is to provide information on the quality of Liberica coffee beans. This research is a laboratory experimental research. The long storage time for coffee beans was 36 and 39 weeks. The results of the research showed that the yeast mold rate (AKK) on Liberica coffee beans stored for 39 weeks was greater than those stored for 36 weeks. The increase in AKK during storage is thought to be due to the presence of insects, temperature and humidity factors. A total of three mold isolates were found at week 36 and there were six mold isolates at week 39. The percentage of fungal isolate attacks at both weeks 36 and 39 was dominated by fungi of the Mucorales order (Mucor sp. and Absidia sp.). In the 36th week the attack of Mucor sp. amounted to 36.67% while in the 39th week the Mucor sp attack. 20%. and Absidia sp. 23.33%. Ochratoxin A (OTA) levels from week 36 to week 39 did not increase but instead decreased. This occurs due to OTA degradation by ochratoxin-degrading molds which are thought to be Aspergillus sp. during storage.
Antibacterial Activity Test of the Combination of Bay Leaf (Syzygium polyanthum) Extract and Nanochitosan Against Escherichia coli and Bacillus subtilis Astrid Farmayati; Siti Harnina Bintari; Pramesti Dewi; Dewi Mustikaningtyas
Life Science Vol. 14 No. 1 (2025): May 2025
Publisher : Universitas Negeri Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/unnesjlifesci.v14.i1.24777

Abstract

Indonesia is one of the world’s most biodiverse countries, including in medicinal plants that contain active compounds with potential as sources of modern medicine, particularly for treating bacterial infections. The use of herbal plants as antibacterial agents offers an alternative to overcome antibiotic resistance and the side effects of synthetic drugs. Bay leaf (Syzygium polyanthum) and nanochitosan are known for their antibacterial properties due to their bioactive compounds. However, studies on their combined effects are still limited. This study aimed to evaluate the antibacterial effectiveness of combinations of bay leaf extract (15%, 25%, and 35%) and nanochitosan (0.5% and 1%) against Escherichia coli and Bacillus subtilis. A Completely Randomized Design (CRD) was applied, using sterile distilled water as a negative control and 0.1% chloramphenicol as a positive control. The antibacterial activity test was performed using the Kirby-Bauer disk diffusion method. Results showed the positive control produced inhibition zones of 25.45 ± 1.27 mm for E. coli and 25.05 ± 0.39 mm for B. subtilis. The most effective combination was K3 (35% bay leaf extract and 0.5% nanochitosan), which produced inhibition zones of 12.65 ± 0.52 mm and 12.91 ± 0.55 mm, respectively. Statistical analysis using the Tukey HSD post hoc test confirmed significant differences among treatments. These findings suggest that the combination of bay leaf extract and nanochitosan holds promise as an alternative antibacterial agent
Effect of Retort Sterilization Temperature and Time on the Quality of Vacuum Packed Soft-Boned Milkfish Lusi Maswahyu Andriyani; Sunyoto; Siti Harnina Bintari; Pramesti Dewi; Dewi Mustikaningtyas
Life Science Vol. 15 No. 1 (2026): May 2026
Publisher : Universitas Negeri Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/unnesjlifesci.v15.i1.27873

Abstract

Soft-boned milkfish is a popular processed product of the milkfish (Chanos chanos), but it has a limited shelf life. Retort sterilization technology with vacuum retort pouch packaging can extend shelf life, but it is necessary to study the right combination of temperature and time to maintain product quality. This study aims to analyze the effect of a combination of temperature (110 °C, 121 °C, and 130 °C) and sterilization time (10, 20, and 30 min) on Total Plate Count (TPC), total protein content, and hedonic quality of the product. The parameters analyzed in this study were TPC with two replications (duplicates), total protein content measured by the Biuret method, and hedonic quality assessed through the hedonic test. The results showed that all treatments produced TPC below the SNI 7388:2009 limit of 5 × 105 CFU/g. The 121 ℃ treatment for 30 min maintained a total protein content of 26.17% and TPC value of 1.00 × 10² CFU/g, while the 130 ℃ treatment for 10 min produced a total protein content of 20.85% and TPC value of 1.00 × 10⁰ CFU/g, and produced the most favorable hedonic quality. Therefore, 130 °C for 10 min is recommended as the optimal sterilization temperature and time, because it is able to produce products that are microbiologically safe and preferred by panelists. Moisture content plays a role in influencing TPC value, total protein content, and hedonic quality. It is recommended to research the effect of temperature and sterilization time of soft-boned milkfish retort on the water content of soft-boned milkfish.
Co-Authors A.A. Ketut Agung Cahyawan W Alam, Aulia Nuanza Alamsyah - Alfiyan, Jamil Maulana Zahriyan Andin Vita Amalia, Andin Vita Andiniyati, Fitri Anhari, Saeful Ani Nur Fauziah, Ani Nur Anik Ulfah, Anik Anisa Dyah P Anisa Dyah P Asih, Tri Sri Noor Astrid Farmayati B., F. Putut Martin H. Chatarina Umbul Wahyuni Chatila Maharani Chulia Mubtadiah, Chulia Danang Dwi Saputro DEDDY MUCHTADI Deddy Muchtadi Dewi Mustikaningtyas Dewi Mustikaningtyas Dewi Susilowati Dhimas Fajar Eka Purnama DIMAS WICAKSONO Dina Nur Anggraini Ningrum Dwi Handayani DWI SUSILANINGSIH DWI YULIANTI Dwi Yulianti Dyah Rini Indriyanti Eka - Mukaromah, Eka - Ely Rudyatmi, Ely Endah Peniati Enni Suwarsi Rahayu Evi Widowati Farikhul Ulum Farikhul Ulum Fazat Haniyya, Fazat Fernando, G. S.N. Fidia Fibriana Hadi Nasbey Hayati, Dwi Puji Hayati, Dwi Puji Heni Pujiastuti Ibnul - Mubarok, Ibnul - Ibnul Mubarok Ibnul Mubarok Ibnul Mubarok Ichsani, Nadya Iin Kurnia Iin Kurnia Intan Zainafree, Intan Isnaeni Karunia Annisa Istiani, Fahrun Istiani, Fahrun Jamil Maulana Zahriyan Alfiyan Jayanti, Septi Jumiyati - Jumiyati - Kartika Nugraheni Kartika Nugraheni Khairunisa, Nisrina L. Putri, Afra Fauziah Lail Isro, Abda Latipah, Rina April Liliek Pratiwi Lisdiana Lisdiana Lusi Maswahyu Andriyani MADE ASTAWAN Mafaza Khaisuntaha Mafaza Khaisuntaha Maftuhah, Anis Margareta Rahayuningsih Nugroho Edi K Martien Herna Susanti Maryani Suwarno Maryani Suwarno Maryani Suwarno Masru'ah, Masru'ah Milah, Nihayatul Moeis, Siti Fatimah Muhammad Agus Muljanto Muhammad Khumaedi Mursyid . Mursyid Mursyid Nadya Ichsani Najah, Khoirun Nana Kariada Trimartuti Natalia Desy Putriningtyas, Natalia Desy Natalia, Lidya Ayu Nicky, Dhea Niken Subekti Nikmatul Hidayah Ning Setiati Ning Setiati Ning Setiati Ningtyas, Mega Mawarni Eva Noor Aini Habibah Nugraini, Anisa Ratna Nurika, Nurika P. Eko Prasetyo Parmin - Pramesti Dewi Pramesti Dewi Prananta, Widya Pratama, Rizka Nur Priyantini Widiyaningrum Puput Roy Purnawati R. Susanti R. Susanti Rachmadi, Moch Faizal Rahina Nugrahani Rahmawati, Fahmi Ramadani, Mutiara Bintang Retno - Istifarini, Retno - Retno Sri Iswari Rivana Citra r Rivana Citra R Rohman, Shohihatur Rosidah Rosidah Sadikin, Nadya Audina Nurkhafiya Saiful Ridlo Sarjadi Sarjadi Sarjadi Sarjadi Siswahyudianto Siti Alimah Siti Fatimah Moeis Siti Maesaroh Sri Sukaesih Sri Sukaesih Sri Utami Sri Widowati Sri Widowati Stephani Diah Pamelasari, Stephani Diah Sucihatiningsh Dian Wisika Prajanti, Sucihatiningsh Dian Wisika Sudarman Sudarman Sugianto - Sunyoto Sunyoto Sunyoto Sunyoto Sunyoto Sutikno Sutikno Syukron Rizqi, Syukron Talitha Widiatningrum Tiara Linanti, Annisa Tirtaningrum, Fitria Ayu Tri Budi Kurniawan Tri Budi Kurniawan, Tri Budi Tutik Wresdiyati Ufi Saraswati, Ufi Ulfa, Berlina Maika Umaira, Nurunnisa Veronika Eka J Veronika Eka J Widya Hary Cahyati Widyanigrum, Dewi Widyanigrum, Dewi Winanti, Ruri Wiwi Isnaeni Wiwi Isnaini Wulan Christijanti Wulandari K. E, Yenni Tyas Yatin Mulyono Yuliana, Lusia Retno Yuliana, Lusia Retno Yuniarti Yuniarti Yustinus Ulung Anggraito