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All Journal Jurnal Veteriner BIOTROPIA - The Southeast Asian Journal of Tropical Biology Indonesian Journal of Mathematics and Natural Sciences Jurnal Penelitian Pendidikan Lembaran Ilmu Kependidikan Rekayasa: Jurnal Penerapan Teknologi dan Pembelajaran Scientific Journal of Informatics Journal of Biology Education Agriprima : Journal of Applied Agricultural Sciences Unnes Science Education Journal Jurnal Gizi dan Dietetik Indonesia (Indonesian Journal of Nutrition and Dietetics) Jurnal Gizi Klinik Indonesia Biosaintifika: Journal of Biology & Biology Education JURNAL PANGAN Journal of Innovative Science Education Berkala Penelitian Hayati Life Science Proceeding of International Conference on Science, Education, and Technology Bookchapter Alam Universitas Negeri Semarang Prosiding Seminar Nasional Biologi Life Science Journal of Creativity Student
Siti Harnina Bintari
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Computer Science & IT Education Engineering Environmental Science Health Professions Immunology & microbiology Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Nursing Public Health Social Sciences Veterinary Other

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Penentuan Konsentrasi Minimum Ekstrak Daun Anting-Anting (Acalypha indica L.) sebagai Antibakteri pada Staphylococcus aureus Alam, Aulia Nuanza; Bintari, Siti Harnina; Mubarok, Ibnul
Life Science Vol 6 No 1 (2017): April 2017
Publisher : Universitas Negeri Semarang

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Abstract

Anting-anting (Acalypha indica, L.) merupakan tumbuhan herba yang dapat dimanfaatkan sebagai antibakteri, karena mengandung senyawa metabolit sekunder salah satunya adalah flavonoid. Potensi tersebut dapat dimanfaatkan untuk mengatasi bakteri resisten akibat antibiotik sintetik yang tidak terkontrol penggunaannya. Salah satu bakteri yang banyak mengalami resistensi adalah bakteri Staphylococcus aureus. Tujuan penelitian ini adalah untuk menentukan konsentrasi minimum ekstrak daun anting-anting sebagai antibakteri S. aureus. Sampel yang digunakan adalah daun anting-anting yang diperoleh dari desa Krasak Pecangaan Jepara. Sampel daun anting-anting diekstrak menggunakan metode soxhletasi kemudian ekstrak diukur kandungan total senyawa flavonoidnya. Ekstrak diujikan terhadap bakteri S. aureus ATCC 29213 dengan variasi konsentrasi ekstrak 20, 40, 60, 80, 100 (mg/mL) selanjutnya diukur zona hambatnya (mm) dengan metode well diffusion. Hasil pengukuran kandungan total senyawa flavonoid ekstrak daun anting-anting diperoleh rata-rata sebesar 18,84 mg QE/gram ekstrak. Zona hambat yang diperoleh dari masing-masing perlakuan berbeda-beda dan semakin meningkat seiring tingginya konsentrasi ekstrak. Rata-rata besar zona hambat dari konsentrasi ekstrak 20, 40, 60, 80, 100 (mg/mL) berturut-turut adalah 14,53; 18,46; 19,46; 20,65; 23,14 (mm). Berdasarkan penelitian tersebut, konsentrasi minimum ekstrak daun anting-anting sebagai antibakteri S. aureus adalah 20 mg/mL setara dengan konsentrasi flavonoid sebesar 0,38 mg QE/gram ekstrak. Acalypha indica L. is herb that be used as antibacterial, do tue they contain secondary metabolites which one of them is flavonoid. This potential can be used to overcome resistant bacteria as a result of uncontrolled synthetic antibiotics usage. One of the bacteria that have many resistances is Staphylococcus aureus. The purpose of this study is to determine the minimum concentration of A. indica L. leaf extract as S. aureus antibacterial. The sample that being used is A. indica L. leaf gained from Krasak village Pecangaan Jepara. The sample of A. indica L. leaves be extracted with soxhlet methode and measured the total flavonoid content. The extracts be tested to S. aureus ATCC 29213 with various concentration of the extract 20, 40, 60, 80, 100 (mg/mL) and inhibitory zone be measured (mm) with well diffusion methode. The results of measurements of total flavonoid content of the extract of the leaves from A. indica L. obtain an average of 18.84 mg QE/gram extract. Inhibitory zone that obtain from each treatment is various and increase along with concentrations of the extract. The average of inhibitory zone from the concentration of 20, 40, 60, 80, 100 (mg/mL) conscutively is 14.53; 18.46; 19.46; 20.65; 23.14 in mm. Based on these studies, the minimum concentration extract A. indica L. leaf an antibacterial S. aureus ATCC 29213 was the treatment of 20 mg/mL which is equivalent to the flavonoid concentration of 0.38 mg QE/gram extract.
Aktivitas Antibakteri Infusa Simplisia Sargassum muticum terhadap Pertumbuhan Staphylococcus aureus Hidayah, Nikmatul; Mustikaningtyas, Dewi; Bintari, Siti Harnina
Life Science Vol 6 No 2 (2017): October 2017
Publisher : Universitas Negeri Semarang

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Abstract

Infeksi yang disebabkan oleh bakteri sering ditemukan di negara tropis yang beriklim panas dan lembab seperti Indonesia. Staphylococcus aureus merupakan bakteri patogen penyebab infeksi kulit yang resisten terhadap beberapa antibiotik. Sargassum muticum dapat digunakan sebagai salah satu alternatif untuk menghambat pertumbuhan Staphylococcus aureus karena mengandung berbagai senyawa metabolit sekunder salah satunya fenol. Tujuan penelitian adalah untuk mengetahui aktivitas antibakteri beberapa konsentrasi infusa simplisia Sargassum muticum terhadap pertumbuhan Staphylococcus aureus. Konsentrasi infusa simplisia yang digunakan (%) yaitu 0, 1, 2, 3, 4, 5, 10 dan sebagai kontrol terdiri dari kontrol positif dan negatif. Data yang diperoleh berupa uji aktivitas antibakteri infusa simplisia S. muticum terhadap pertumbuhan S. aureus dan kandungan total fenol simplisia S. muticum dianalisis secara deskriptif, serta analisis korelasi secara statistik untuk mengetahui hubungan kedua variabel uji. Hasil penelitian menunjukkan adanya peningkatan kandungan total fenol seiring bertambahnya konsentrasi simplisia S. muticum. Konsentrasi 1-5% tidak menunjukkan aktivitas antibakteri, sedangkan konsentrasi 10% menunjukkan aktivitas antibakteri dengan membentuk hambatan sebesar 21 mm. Analisis korelasi positif dengan koefisien korelasi 0,577 artinya semakin tinggi aktivitas antibakteri maka kandungan fenol yang terdapat pada simplisia S. muticum juga semakin tinggi. Berdasarkan hasil penelitian dapat disimpulkan bahwa infusa simplisia S. muticum mulai menunjukkan aktivitas antibakteri terhadap S. aureus ATCC 29213 pada konsentrasi 10%. Infection caused by bacteria commonly found in tropical hot climates and moist like Indonesia. Staphylococcus aureus is a pathogenic bacteria responsible for skin infections that can resistant to several antibiotics. Sargasum muticum has been used as an alternative to inhibit the growth of Staphylococcus aureus, because it contains a variety of secondary metabolite compounds such as phenols. The aim of this study was to determine the antibacterial activity several concentration of the infuse powder Sargasum muticum against Staphylococcus aureus The concentration of the infuse powder used (%): 0%, 1%, 2%, 3%, 4%, 5%, 10% and as a control consisting of a positive control, and negative control. The data consist of antibacterial activity of S. muticum infuse powder against S. aureus and total phenol content of S. muticum was analyzed descriptively, statistically test correlation analysis to determine the relationship between two variables tested. The results showed an increase in total phenol content with increased concentrations of S. muticum. 1-5% concentration of infuse powdered did not show antibacterial activity, while at 10% concentration showed antibacterial activity by forming a barrier diameter of 21 mm. Analysis result of correlation is positive, with a correlation coefficient 0.577, it means that higher antibacterial activity, content of phenols in S. muticum powdered are also higher. Based on the research, it concluded that the infuse powder of Sargassum muticum showed antibacterial activity against S. aureus ATCC 29213 at a concentration of 10%.
Deteksi Escherichia coli Pada Jamu Gendong di Gunungpati dengan Medium Selektif Diferensial Utami, Sri; Bintari, Siti Harnina; Susanti, R.
Life Science Vol 7 No 2 (2018): November 2018
Publisher : Universitas Negeri Semarang

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Abstract

Jamu gendong termasuk dalam kategori obat herbal yang dikonsumsi untuk menjaga kesehatan. Kontaminasi Escherichia coli pada produk jamu gendong dapat mempengaruhi manfaat jamu gendong sebagai obat herbal. Penelitian ini bertujuan untuk mendeteksi keberadaan bakteri E. coli pada sampel jamu gendong jenis beras kencur dan kunyit asam di Kecamatan Gunungpati Semarang. Penelitian ini merupakan penelitian observasional dengan sampel diambil secara acak. Sebanyak sebelas sampel jamu beras kencur dan kunyit asam dari perajin jamu gendong diuji menggunakan medium selektif diferensial Eosin Methylene Blue Agar. Sampel positif terkontaminasi E. coli pada medium EMBA ditunjukkan dengan adanya koloni berwarna gelap dengan kilap hijau metalik. Data yang diperoleh dari hasil uji keberadaan E. coli, perhitungan jumlah koloni E. coli dan lembar observasi dianalisis secara deskriptif. Penelitian ini menunjukkan bahwa dari sebelas sampel jamu gendong yang diuji, sembilan sampel beras kencur dan tiga sampel kunyit asam positif terkontaminasi E. coli. Hasil perhitungan jumlah koloni bakteri E. coli diperoleh sembilan sampel beras kencur dan dua sampel kunyit asam tidak memenuhi aturan batas cemaran mikroba dalam Standar Nasional Indonesia. ‘Jamu gendong’ included in herbal medicine category which consumed to maintain health. Escherichia coli contamination in ‘jamu gendong’ products can affect the benefits as an herbal medicine. This study aims to detect the presence of E. coli bacteria in the sample of ‘jamu gendong’ type of ‘beras kencur’ and ‘kunyit asam’ in Gunungpati District Semarang. This research is an observational study with random sampling. A total of eleven samples of ‘jamu gendong’ of ‘beras kencur’ and ‘kunyit asam’ from ‘jamu gendong’ sellers were tested using differential selective medium of Eosin Methylene Blue Agar. Positive samples which contaminated with E. coli in the EMBA medium are indicated by the presence of dark colored colonies with metallic green luster. Data obtained from the results of the test of the presence of E. coli, calculation of the number of E. coli colonies and observation sheets were analyzed descriptively. This study showed that of the eleven samples of ‘jamu gendong’ that were tested, nine samples of ‘beras kencur’ and three samples of ‘kunyit asam’ were positively contaminated with E. coli. The results of the calculation of the number of colony of E. coli bacteria obtained nine samples of ‘beras kencur’ and two samples of ‘kunyit asam’ did not meet the rules for the limits of microbial contamination in the Indonesian National Standard.
Analisis Perkembangan Titer Antibodi Hasil Vaksinasi Infectious Bronchitis pada Ayam Petelur Strain Hisex Brown Wulandari K. E, Yenni Tyas; Susanti, R.; Bintari, Siti Harnina
Life Science Vol 8 No 1 (2019): April 2019
Publisher : Universitas Negeri Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/lifesci.v8i1.29987

Abstract

Infectious bronchitis (IB) is a disease that attacks the chicken's respiratory system caused by an infectious bronchitis virus. IB transmission can occur directly and indirectly. IB disease transmission can be done by vaccination. Vaccination can fail so that to determine the success of the vaccination program an antibody titer monitor is performed using serological tests. ELISA is one of the serological tests that can be used to measure antigens/antibodies. The main principle of the ELISA technique is the use of enzyme indicators for immunological reactions. This study aims to analyze the differences in chicken antibody titers at certain periods after vaccination. The sample in this study used 18 female layer hens of Hisex Brown Strain aged 20 weeks that were vaccinated by IB. This study was an experimental study with treatment of antibody titer measurements at 7, 14, and 21 days after vaccination. Data analysis is descriptive quantitative using the Normality test and t test. The mean results of antibody titers at each consecutive time period at 7, 14, and 21 days after vaccination were 1695, 4207, and 5978. The results of the t test showed that at each sampling period 7 dpi, 14 dpi and 21 dpi had differences significant. The number of titers of IgG antibodies to the IBV virus increases from a distance after vaccination. Conclusions were obtained that antibodies increased in each period of time taken after vaccination. Penyakit Infectious bronchitis (IB) adalah penyakit yang menyerang sistem pernafasan ayam yang disebabkan oleh Infectious bronchitis virus. Penularan IB dapat terjadi secara langsung dan tidak langsung. Penularan penyakit IB dapat dilakukan dengan cara vaksinasi. Vaksinasi dapat mengalami kegagalan sehingga untuk mengetahui keberhasilan program vaksinasi dilakukan monitor titer antibodi menggunakan uji serologis. ELISA adalah salah satu uji serologis yang dapat digunakan untuk mengukur antigen/antibodi. Prinsip utama teknik ELISA adalah penggunaan indikator enzim untuk reaksi imunologi. Penelitian ini bertujuan menganalisis adanya perbedaan titer antibodi ayam pada periode tertentu setelah vaksinasi. Sampel pada penelitian ini menggunakan 18 ekor ayam petelur betina Strain Hisex Brown umur 20 minggu yang divaksin IB. Penelitian ini merupakan penelitian eksperimental dengan perlakuan pengukuran titer antibodi pada 7, 14, dan 21 hari setelah vaksinasi. Analisis data secara deskriptif kuantitatif menggunakan uji Normalitas dan uji t. Rerata hasil titer antibodi pada setiap periode waktu berturut-turut pada 7, 14, dan 21 hari setelah vaksinasi yaitu 1695, 4207, dan 5978. Hasil uji t menunjukkan pada setiap periode pengambilan sampel yaitu 7 dpi, 14 dpi, dan 21 dpi mempunyai perbedaan yang signifikan. Jumlah titer antibodi IgG terhadap virus IBV semakin meningkat dari jarak setelah vaksinasi. Kesimpulan yang diperoleh bahwa antibodi mengalami peningkatan pada setiap periode waktu pengambilan setelah vaksinasi.
Pengaruh Pemberian Pakan dengan Penambahan Overripe Tempeh terhadap Konsumsi Pakan, Pertambahan Bobot Badan dan Konversi Pakan pada Ayam Petelur L. Putri, Afra Fauziah; Bintari, Siti Harnina
Life Science Vol 10 No 1 (2021): April 2021
Publisher : Universitas Negeri Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/lifesci.v10i1.47168

Abstract

Overripe tempeh is soybean tempeh which has undergone continuous fermentation. The use of overripe tempeh as a food ingredient in making traditional dishes was not done optimally. In fact, overripe tempeh contains isoflavones and probiotic compounds which are considered to improve the quality of animal feed. Isoflavone compounds contained in overripe tempeh have phytoestrogen properties in increasing the productivity of laying hens. This study aims to examine the effect of feeding with the addition of overripe tempeh with different concentrations on feed consumption, body weight gain, and feed conversion. This research was an experimental study with a completely randomized design (CRD) with 4 treatments and 7 replications. The research material used 28 layers of 94 week old laying hens. The feed treatment with the addition of overripe tempeh consisted of P0 (feed without overripe tempeh), P1 (7.5% addition of overripe tempeh), P2 (15% addition of overripe tempeh), and P3 (22.5% addition of overripe tempeh). The parameters observed consisted of feed consumption, body weight gain, and feed conversion. The data from the research results were analyzed for a variance with the F test at the 5% level. The results of the variance analysis showed that laying hens had a significant effect on feed consumption, but had no significant effect on body weight gain and feed conversion. It can be concluded that the use of tempeh overripe can be used up to 7.5% in layer hens feed. Keywords: overripe tempeh, layer chicken, performances, overripe tempeh, ayam petelur, performans. Overripe tempeh merupakan tempe kedelai yang telah mengalami fermentasi yang berkelanjutan. Pemanfaatan overripe tempeh sebagai bahan makanan dalam penambahan masakan tradisional tidak dilakukan secara optimal. Padahal, overripe tempeh memiliki kandungan senyawa isoflavon dan kandungan probiotik yang dinilai dapat meningkatkan mutu pakan hewan ternak. Senyawa isoflavon yang terkandung didalamnya memiliki sifat fitoestrogen sehingga dapat meningkatkan produktivitas ayam petelur. Penelitian ini bertujuan untuk mengkaji pengaruh pemberian pakan dengan penambahan overripe tempeh dengan konsentrasi yang berbeda terhadap konsumsi pakan, pertambahan bobot badan dan konversi pakan. Penelitian yang dilakukan menggunakan rancangan acak lengkap (RAL) dengan 4 perlakuan dan 7 ulangan. Materi penelitian menggunakan ayam petelur umur 94 minggu sebanyak 28 ekor. Perlakuan pakan dengan penambahan overripe tempeh terdiri dari P0 (tanpa overripe tempeh), P1 (7,5% penambahan overripe tempeh), P2 (15% penambahan overripe tempeh), dan P3 (22,5% penambahan overripe tempeh). Parameter yang diamati terdiri dari konsumsi pakan, pertambahan bobot badan, dan konversi pakan. Data hasil penelitian dianalisis ragam dengan uji F pada taraf uji 5%, jika berpengaruh nyata dilanjutkan uji BNJ (5%). Hasil analisis ragam menunjukkan bahwa ayam petelur berpengaruh nyata terhadap konsumsi pakan, namun tidak berpengaruh nyata terhadap pertambahan bobot badan dan konversi pakan. Dapat disimpulkan bahwa penggunaan overripe tempeh dapat digunakan hingga 7,5% dalam pakan ayam petelur. Keywords: overripe tempeh, layer chicken, performances, overripe tempeh, ayam petelur, performans.
Isolasi, Karakterisasi, dan Uji Aktivitas Antibakteri dari Bakteri Endofit Daun Kelor (Moringa oleifera) Sadikin, Nadya Audina Nurkhafiya; Bintari, Siti Harnina; Widiatningrum, Talitha; Dewi, Pramesti
Life Science Vol 10 No 2 (2021): November 2021
Publisher : Universitas Negeri Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/lifesci.v10i2.54441

Abstract

Endophytic bacteria are beneficial microorganisms that interact with host plants without causing any interference or damage to plants. Moringa oleifera is a medicinal plant that contains secondary metabolites and has antibacterial properties. The aim of this research is to isolate and characterize endophytic bacteria from Moringa oleifera leaves, and to test the antibacterial activity against pathogenic bacteria (Escherichia coli and Bacillus subtilus). The results of the isolation of Moringa leaf endophytic bacteria (Moringa oleifera) obtained amounted to one isolate. Based on the results of the catalase test, gram staining and spore observation, moringa oleifera leaf endophytic bacteria included in the genus Bacillus. The antibacterial test begins with the production of secondary metabolites of Moringa oleifera leaf endophytic bacteria, measures the rate of bacterial growth and tests the antibacterial activity using the Kirby Bauer method with paper disks. Observational data were analyzed statistically using ANOVA and further tests with Duncan (sig <0.05). Antibacterial activity test results showed that the incubation time of endophytic bacteria affected the growth of Escherichia coli and Bacillus subtilus bacteria. The incubation time of 10 hours showed the highest inhibition zone diameter of Escherichia coli and Bacillus subtilus bacteria respectively of 7.5 mm and 1.8 mm. The formation of inhibitory zones indicates the presence of secondary metabolite compounds from Moringa leaf endophytic bacteria which have an antibacterial effect. Bakteri endofit merupakan mikroorganisme menguntungkan yang berinteraksi dengan tanaman inang tanpa menyebabkan gangguan atau kerusakan pada tanaman. Kelor (Moringa oleifera) merupakan tanaman obat yang mengandung metabolit sekunder dan berkhasiat sebagai antibakteri. Tujuan penelitian ini adalah mengisolasi dan mengkarakterisasi bakteri endofit dari daun kelor, serta menguji aktivitas antibakteri terhadap bakteri patogen (Escherichia coli dan Bacillus subtilus). Hasil isolasi bakteri endofit daun kelor (Moringa oleifera) yang diperoleh berjumlah satu isolat. Berdasarkan hasil uji katalase, pewarnaan gram dan pengamatan spora, bakteri endofit daun kelor termasuk ke dalam genus Bacillus. Uji antibakteri dimulai dengan produksi metabolit sekunder bakteri endofit daun kelor, pengukuran laju pertumbuhan bakteri dan uji aktivitas antibakteri menggunakan metode Kirby bauer dengan paper disk. Data hasil pengamatan dianalisis statistik menggunakan ANOVA dan uji lanjut dengan Duncan (sig < 0.05). Hasil uji aktivitas antibakteri menunjukkan bahwa waktu inkubasi bakteri endofit berpengaruh terhadap pertumbuhan bakteri Escherichia coli dan Bacillus subtilus. Waktu inkubasi selama 10 jam menunjukan diameter zona hambat tertinggi terhadap bakteri Escherichia coli dan Bacillus subtilus berturut-turut sebesar 7.5 mm dan 1.8 mm. Terbentuknya zona hambat mengindikasikan adanya senyawa metabolit sekunder dari bakteri endofit daun kelor yang memiliki efek antibakteri.
PENERAPAN IPTEK USAHA PEMBUATAN TAHU DAN TEMPE DI BANDUNGAN KABUPATEN SEMARANG Sunyoto, Sunyoto; Rosidah, Rosidah; Bintari, Siti Harnina
Rekayasa : Jurnal Penerapan Teknologi dan Pembelajaran Vol 12, No 1 (2014): Juli 2014
Publisher : Universitas Negeri Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/rekayasa.v12i1.5583

Abstract

The purpose of this activity is to resolve the problems faced by the industry partner with the business of making tofu and tempeh, which is located in the village of Kenteng, Bandungan subdistrict, Semarang regency. As this activity involves the implementation team of lecturers with expertise appropriate to the needs of the field and assisted students. In the implementation used multiple methods of mutual support, with a practice emphasis directly by partners and mentoring activities. The results of these activities include 1) One unit of soybean grinding machine with an electric motor drive power 1 HP, 2) One unit of soybean crusher drive motor gasoline of 5.5 PK and has a production of capacity of 300 kg / hour, or five times that of soy-solving process manually which is only 60 kg / h, 3) Both the partners I and II have applied with better production management, and a cleaner production equipment, and workers had to use health and safety equipment. Impact activities include economic and non-economic aspects. The economic impact can be measured from the increased productivity and better product quality so as to increase the selling value of products. Non-economic impact can be measured from the comfort of work will also indirectly affect the economic benefits.
PENERAPAN IPTEK PADA INDUSTRI TAHU KHAS BANDUNGAN KABUPATEN SEMARANG Khumaedi, Muhammad; Sudarman, Sudarman; Bintari, Siti Harnina
Rekayasa : Jurnal Penerapan Teknologi dan Pembelajaran Vol 13, No 1 (2015): Juli 2015
Publisher : Universitas Negeri Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/rekayasa.v13i1.5594

Abstract

Science and technology activities for the people (IbM) was conducted with the aim of resolving the problems faced by tofu small industries in Bandungan, Semarang regency, Central Java. There are two IbM partners, namely Mrs. Biyati and Mrs. Widari. There are two aspects of the problems to be addressed, namely the aspects of production and management. As a team implementing these activities are lectures and students with expertise in accordance with the needs of the field. In the implementation of some of the methods that are used to support, among others, with lectures for the theoretical material and motivation, demonstration to deliver material practice / skills, practice directly by the partners, and mentoring activities. In this activity has generated output production and management aspects. Outputs aspects of production: 1) soy grinding machine with a gasoline motor power source so that the sound is smooth and does not interfere with citizens, 2) equipment using steam boiler (steam boiler) so that the kitchen space free of smoke, knows not of bitter and feel better, 3 ) Stamping tools screw system making it easy to operate / lighter. Outputs management aspects: 1) bookkeeping financial / business better, and 2) further increased marketing / marketing a wider range.
PENERAPAN IPTEK PADA USAHA KUE KERING DESA BUGO KABUPATEN JEPARA Setiati, Ning; Bintari, Siti Harnina; Christijanti, Wulan
Rekayasa : Jurnal Penerapan Teknologi dan Pembelajaran Vol 13, No 1 (2015): Juli 2015
Publisher : Universitas Negeri Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/rekayasa.v13i1.5595

Abstract

Objectives to be achieved in service activities is to foster the spirit of starting a new life for the 5 farmers who suffered catastrophic cold lava flood particular group Livestock quail Tani Mulyo Hamlet Quick Village Gondosuli Muntilan district and 5 breeders are joined in Group Livestock quail Rejeki Hamlet Macanan Banyudono Shaman District. Specific targets are the seeds quail ready to lay that out of 1,000 head of targeted quail egg can produce 75%, assuming a quail egg-laying 1 egg / day, sold at a retail price of Rp. 210, - / point. Results of these activities are still limited availability of seedling 3,690 tails with an average weight increase in the age of 2 weeks 28 grams and 3,450 head at the age of 8 weeks to 167 grams. Egg production reached 86% before the arranged marriage which only reached 66% egg production quail. To foster the spirit of entrepreneurship that is able to think of creative and innovative and able to read the opportunities as well as clever use it in accordance with the capabilities and not focused on just one type of work just the wives of farmers tried to create diversified products made from meat and quail eggs.
Evaluasi Keamanan Tempe dari Kedelai Transgenik Melalui Uji Subkronis pada Tikus (SAFETY EVALUATION OF TEMPE MADE FROM TRANSGENIC SOYBEAN USING SUBCHRONIC TEST ON RATS) Maryani Suwarno; Made Astawan; Tutik Wresdiyati; Sri Widowati; Siti Harnina Bintari; Mursyid .
Jurnal Veteriner Vol 15 No 3 (2014)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

The consumption need of soybean that reached up to 2,5-3 million tons per year has made Indonesiaimports this commodity 1,95 million tons from countries that adopt Genetically Modified (GM) soybean.Soybean utilization for tempe producers is about 50% from total its supply. The aim of this research wasto evaluate the safety of tempe made from GM soybean, by a subchronic test approach using male Sprague-Dawley rats. Toxicity evaluation of tempe from soybean GM was done based on EFSA (2011) principalusing animal laboratory. For 90 days, three groups of male rats (each group contain seven rats) werefeeding with tempe flour from GM soybean, tempe flour from non-GM soybean and control group wasfeeding with casein as a protein source. All rats were terminated on day 90,where the blood, liver andkidney were collected. There were no significant differences on liver and kidney weight between treatment.Hematology analysis showed the level of Hb, erythrocyte, leucocyte, trombocyte and hematocryte were notsignificantly different in between group. Serum analysis on SGOT, SGPT, total protein, albumin, globulin,blood glucose, triglyceride, ureum and creatinin level were also showed no difference between groups. As aconclusion, the consumption of tempe derived from GM soybean was safe, and cause no adverse effect onhealth.
Co-Authors A.A. Ketut Agung Cahyawan W Alam, Aulia Nuanza Alamsyah - Alfiyan, Jamil Maulana Zahriyan Anam, M. Khairul Andin Vita Amalia, Andin Vita Anhari, Saeful Ani Nur Fauziah, Ani Nur Anik Ulfah, Anik Anisa Dyah P Anisa Dyah P Astrid Farmayati Caska - Chulia Mubtadiah, Chulia Danang Dwi Saputro Deddy Muchtadi DEDDY MUCHTADI Dewi Mustikaningtyas Dewi Susilowati Dhimas Fajar Eka Purnama Dwi Handayani DWI SUSILANINGSIH DWI YULIANTI Dyah Rini Indriyanti Eka - Mukaromah, Eka - Ely Rudyatmi, Ely Endah Peniati Enni Suwarsi Rahayu Evi Widowati Farikhul Ulum Farikhul Ulum Fazat Haniyya, Fazat Fernando, G. S.N. Fidia Fibriana Fitri Andiniyati Hadi Nasbey Hayati, Dwi Puji Hayati, Dwi Puji Heni Pujiastuti Ibnul - Mubarok, Ibnul - Ibnul Mubarok Ibnul Mubarok Ibnul Mubarok Ichsani, Nadya Iin Kurnia Iin Kurnia Intan Zainafree, Intan Isnaeni Karunia Annisa Istiani, Fahrun Istiani, Fahrun Jamil Maulana Zahriyan Alfiyan Jayanti, Septi Jumiyati - Jumiyati - Kartika Nugraheni Kartika Nugraheni Khairunisa, Nisrina L. Putri, Afra Fauziah Lail Isro, Abda Latipah, Rina April Lisdiana Lisdiana MADE ASTAWAN Mafaza Khaisuntaha Mafaza Khaisuntaha Maftuhah, Anis Margareta Rahayuningsih Nugroho Edi K Martien Herna Susanti Maryani Suwarno Maryani Suwarno Maryani Suwarno Masru'ah, Masru'ah Milah, Nihayatul Moeis, Siti Fatimah Muhammad Agus Muljanto Muhammad Khumaedi Mursyid . Mursyid Mursyid Mutiara Bintang Ramadani Nadya Ichsani Najah, Khoirun Nana Kariada Trimartuti Natalia Desy Putriningtyas, Natalia Desy Natalia, Lidya Ayu Nicky, Dhea Niken Subekti Nikmatul Hidayah Ning Setiati Ning Setiati Ning Setiati Nugraini, Anisa Ratna Nurika, Nurika P. Eko Prasetyo Parmin - Pramesti Dewi Pratama, Rizka Nur Priyantini Widiyaningrum Puput Roy Purnawati R. Susanti R. Susanti Rahina Nugrahani Rahmawati, Fahmi Retno - Istifarini, Retno - Retno Sri Iswari Rivana Citra r Rivana Citra R Rohman, Shohihatur Rosidah Rosidah Sadikin, Nadya Audina Nurkhafiya Saiful Ridlo Sarjadi Sarjadi Sarjadi Sarjadi Siti Alimah Siti Fatimah Moeis Siti Maesaroh Sri Sukaesih Sri Sukaesih Sri Utami Sri Widowati Sri Widowati Stephani Diah Pamelasari, Stephani Diah Sucihatiningsh Dian Wisika Prajanti, Sucihatiningsh Dian Wisika Sudarman Sudarman Sunyoto Sunyoto Sunyoto Sunyoto Syukron Rizqi, Syukron Talitha Widiatningrum Tiara Linanti, Annisa Tirtaningrum, Fitria Ayu Tri Budi Kurniawan Tri Budi Kurniawan, Tri Budi Tutik Wresdiyati Veronika Eka J Veronika Eka J Widya Hary Cahyati Widyanigrum, Dewi Widyanigrum, Dewi Winanti, Ruri Wiwi Isnaini Wulan Christijanti Wulandari K. E, Yenni Tyas Yanuar Revandi Yatin Mulyono Yuliana, Lusia Retno Yuliana, Lusia Retno Yuniarti Yuniarti Yustinus Ulung Anggraito