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All Journal Jurnal Veteriner BIOTROPIA - The Southeast Asian Journal of Tropical Biology Indonesian Journal of Mathematics and Natural Sciences Jurnal Penelitian Pendidikan Lembaran Ilmu Kependidikan Rekayasa: Jurnal Penerapan Teknologi dan Pembelajaran Scientific Journal of Informatics Journal of Biology Education Agriprima : Journal of Applied Agricultural Sciences Unnes Science Education Journal Jurnal Gizi dan Dietetik Indonesia (Indonesian Journal of Nutrition and Dietetics) Jurnal Gizi Klinik Indonesia Biosaintifika: Journal of Biology & Biology Education JURNAL PANGAN Journal of Innovative Science Education Berkala Penelitian Hayati Life Science Proceeding of International Conference on Science, Education, and Technology Bookchapter Alam Universitas Negeri Semarang Prosiding Seminar Nasional Biologi Life Science Journal of Creativity Student
Siti Harnina Bintari
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Computer Science & IT Education Engineering Environmental Science Health Professions Immunology & microbiology Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Nursing Public Health Social Sciences Veterinary Other

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The Potential of Microbial Symbionts Macrotermes gilvus Hagen Termite Gut as Degrading Agents of Cellulose in Bioethanol Production Dewi Susilowati; Niken Subekti; Siti Harnina Bintari
Biosaintifika: Journal of Biology & Biology Education Vol 10, No 2 (2018): August 2018
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v10i2.14965

Abstract

Water hyacinth is a potential feedstock for bioethanol production because of their high cellulose. The microbial symbionts of the Macrotermes gilvus termite’s gut have a high endoglucanase enzyme activity. This research was aimed to analyze the pH, temperature and agitation effects towards cell density, endoglucanase enzyme activity and reducing sugar, and to determine the effective optimum condition that can produce maximum reducing sugar. This research used central composite design (CCD) with the total number of run was . The independent variables were including pH (5.9, 6.4, 7.0, 7.6, 8.0), temperature (30 0C, 33 0C, 37 0C, 41 0C, 44 0C) and agitation (90 rpm,114 rpm, 150 rpm,185 rpm, 210 rpm), with six replications at central points. Parameters measured were cell density, endoglukanase enzyme activity and reducing sugar, thus analyzed by the statistical software package MINITAB 18.0. The Student’s t-test result showed the primary sequence influencing cell density as pH ˃ agitation ˃ temperature and towards endoglucanase enzyme activity and reducing sugar as pH ˃ temperature ˃ agitation, P ˂ 0.05. The maximum reducing sugar (60.13 ± 3.16 mmolL-1) was obtained at pH 6.95, temperature 37 0C and agitation 150 rpm. The results of this research can be used to explore the more potential microbial symbionts of the Macrotermes gilvus Hagen termite’s gut.
The Density and Viability of Metarhizium anisopliae Conidia on Several Growth Media Dyah Rini Indriyanti; Siti Harnina Bintari; Ning Setiati; Jamil Maulana Zahriyan Alfiyan
Biosaintifika: Journal of Biology & Biology Education Vol 13, No 2 (2021): August 2021
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v13i2.31408

Abstract

Metarhizium anisopliae is a parasitic fungus on insects, and thus called entomopathogenic fungus. This fungus is used as a biological control agent for insect pests. Fungal propagation can be done using a variety of media. The purpose of this study was to analyze the growth of M. anisopliae on four types of media, with conidial density and viability as the growth parameters. This research was conducted at the Laboratory of Microbiology, Universitas Negeri Semarang. This study was an experimental research used a Completely Randomized Design (CRD) with one factor and four treatments: Control (PDA/Potato Dextrose Agar medium), Treatment I (ELSA/Extract Larvae Sucrose Agar medium), Treatment II (CWSA/Coconut Water Sucrose Agar medium), Treatment III (CWELSA/Coconut Water and Extract Larvae Sucrose Agar medium). The and results showed that there was an effect of growth media on the density and viability of M. anisopliae conidia. CWELSA media had the highest conidial density (2.91 x 108 cfu/mL) and viability (97.17%). CWSA media had  2.82 x 108 cfu/mL and 95.33%. PDA media had 2.25 x 108 cfu/mL and 92.83%. ELSA media had 1.64 x 108 cfu/mL and 90.83%. The high conidial density and viability of M. anisopliae is CWELSA  medium. This study is as an alternative growth medium to improve the quality of M. anisopliae propagation.
Microbiological and Biochemical Tests on Tempe Production Using Tempe Mold Innovation Siti Harnina Bintari; Dhimas Fajar Eka Purnama; Danang Dwi Saputro; Sunyoto Sunyoto; Pramesti Dewi; Ibnul Mubarok
Biosaintifika: Journal of Biology & Biology Education Vol 14, No 2 (2022): August 2022
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v14i2.37700

Abstract

Tempe is a popular fermented food in Indonesia, one of the important things in the process of making tempeh is the tempe incubation container. The purpose of this study was to prove the quality of tempe products made using the tempe mold innovation suitable for production, to analyze the comparison between the quality of tempe products made using a tempe incubation tool and without using the tool. Experimental research with Completely Randomized Design (CRD), applying a tempe incubator and without using a tool as a control. Test methods used include testing water content, hygiene, number of mold colonies and protein content. Based on the results of the study, tempe products made using a tempe printer contain a moisture content of 51.4 - 56.2%, protein content 17.92 - 18.58%, the number of mold colonies 3.65 x 105 untill 4.08 x 105 cell/gr and negative Escherichia coli. Based on the results of the study, it was concluded that the tempe products made using the tempe mold innovation tool had met the biological and chemical quality standards based on SNI 3144:2015 and had an overall quality test result that was superior to the control.
ISOLASI DAN IDENTIFIKASI KHAMIR SECARA MORFOLOGI DI TANAH KEBUN WISATA PENDIDIKAN UNIVERSITAS NEGERI SEMARANG Jumiyati -; Siti Harnina Bintari; Ibnul Mubarok
Biosaintifika: Journal of Biology & Biology Education Vol 4, No 1 (2012): March 2012
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v4i1.2265

Abstract

Tujuan penelitian untuk mengisolasi dan mengidentifikasi khamir secara morfologi di tanah Kebun Wisata Pendidikan Unnes. Penelitian ini merupakan penelitian eksplorasi yang dilakukan di Laboratorium Mikrobiologi Jurusan Biologi FMIPA Unnes. Populasi penelitian adalah khamir di tanah Kebun Wisata Pendidikan Unnes. Sampel penelitian adalah isolat khamir yang diambil dengan teknik Purposive Sampling yaitu membagi kebun menjadi lima zona dan setiap zona diambil secara acak lima titik pengambilan kemudian dihomogenkan. Sampel dibawa ke Laboratorium untuk dilakukan isolasi, purifikasi dan identifikasi secara morfologi koloni dan sel. Simpulan penelitian ditemukan tujuh isolat khamir dan termasuk ke dalam lima genus yaitu Saccharomyces, Candida, Debaromyces, Brettanomyces dan Saccharomycodes. The purpose of the research was to isolate and to identify morphologically the individually-isolated yeasts from the soil of Biology Educational Garden Semarang State University. The exploration research was conducted in the Microbiology Laboratory Department of Biology, FMIPA Semarang State University. The yeasts were collected using purposive sampling technique in five zones and from each zone five random plots were selected to obtain the samples. Samples were isolated, purified and identified morphologically in terms of the colonies and the cells. Result revealed that seven isolated yeasts from five generas (Saccharomyces, Candida, Debaromyces, Brettanomyces, and Saccharomycodes) had been collected from the soil of Biology Educational Garden Semarang State University.
Isolasi dan Identifikasi Virus Avian Influenza Subtipe H5N1 pada Unggas di Pasar Tradisional Semarang Farikhul Ulum; R. Susanti; Siti Harnina Bintari
Biosaintifika: Journal of Biology & Biology Education Vol 5, No 2 (2013): September 2013
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v5i2.3914

Abstract

Meningkatnya kasus infeksi virus Avian Influenza (AI) subtipe H5N1 atau lebih dikenal dengan flu burung yang menyebabkan kematian pada manusia sangat dikhawatirkan dapat menular dari manusia ke manusia. Penelitian ini bertujuan untuk mendapatkan isolat virus Avian Influenza subtipe H5N1 pada unggas yang diperjualbelikan di pasar tradisional di Semarang. Sebanyak 55 sampel usap kloaka diambil dari unggas sehat dan belum divaksin di 6 pasar tradisional Kota Semarang. Inokulum ditumbuhkan pada telur ayam berembrio specific pathogen free (TAB-SPF) umur sembilan hari. Kemudian telur diinkubasikan selama 4 hari. Cairan alantois dipanen dan diuji kemampuannya mengaglutinasi sel darah merah. Cairan alantois yang menunjukkan aktivitas hemaglutinasi, selanjutnya diekstraksi RNA-nya dan diidentifikasi VAI subtipe H5N1 dengan metode Reverse Transcriptase-Polymerase Chain Reaction (RT-PCR) menggunakan primer spesifik H5 dan primer N1. Kemudian DNA hasil RT-PCR dianalisis dengan teknik elektroforesis. Hasil penelitian menunjukkan bahwa terdapat 4 isolat positif VAI subtipe H5N1 dengan sebaran 2 isolat dari sampel yang berasal dari pasar Mangkang, 1 isolat dari pasar Rejomulyo dan 1 isolat dari pasar Karimata. Berdasarkan hasil penelitian dan pembahasan dapat disimpulkan bahwa unggas yang diperjualbelikan di pasar tradisional di Kota Semarang ada yang terinfeksi VAI subtipe H5N1.The increasing cases of viral infection of Avian Influenza (AI) H5N1 subtype or more commonly known as bird flu that causes death in humans very feared to spread from human to human. The aim of this research was to obtain isolates of Avian Influenza virus (AIV) subtype H5N1 that marketable in traditional markets in Semarang. A total of 55 cloacal swab samples taken from healthy and unvaccinated fowl in the 6 traditional market in Semarang. Inoculum was grown in embryonated chicken eggs specific pathogen free (SPF TAB) nine days. Then the eggs were incubated for 4 days. Allantoic fluids were harvested and tested for their ability to agglutinate red blood cells. Allantoic fluid that showed hemagglutination activity, further their RNA was extracted and AIV subtype H5N1 identified with Reverse Transcriptase-Polymerase Chain Reaction (RT-PCR) method using specific primers primary H5 and N1. Then the results of RT - PCR were analyzed by electrophoresis technique. The results showed that there are 4 positive isolates with the distribution of the H5N1 subtype AIV 2 isolates samples derived from market Mangkang, 1 isolate from market Rejomulyo and 1 isolate from market Karimata. Based on the results of research and discussion, it can be concluded that the fowl that marketable in traditional markets in Semarang there were AIV infected with subtype H5N1.
Efek Inokulasi Bakteri Micrococcus luteus Terhadap Pertumbuhan Jamur Benang dan Kandungan Isoflavon pada Proses Pengolahan Tempe (Effect Inoculation of Micrococcus luteus to Growth of Mold and Content Isoflavone at Tempe Processing) Siti Harnina Bintari; Anisa Dyah P; Veronika Eka J; Rivana Citra R
Biosaintifika: Journal of Biology & Biology Education Vol 1, No 1 (2009): March 2009
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v1i1.43

Abstract

Tempe content of aglikon isoflavone which have potency to as anticancer compound. During processing of tempe, beside tempe mold (Rhizopus oligosporus) share other microbial namely bacterium. One of the bacterium residing at tempe ecosystem is Micrococcus luteus, having the character of gram positive and can conduct genistein biotransformation and daidzein become factor II compound (6,7,4-tri hydroksi isoflavone). Its problems how amount of R.oligosporus cell and M. luteus at processing of tempe associated is full scale of yielded tempe isoflavone. The study aimed to improve full scalely tempe aglikon isoflavone through inoculation of M.luteus bacteria. Used complete random designd, with M.luteus bacteria, as independent variable and variable depended is the amount of mold cell (R..oligosporus), amount of full scale M.luteus cell and total of isoflavone. Result of one way Anava indicate that inoculation of M.luteus bacteria do not degrade the amount of R.oligosporus cell with F call (53,28) F tab (3,10) at significancy level 5%. Amount of M.luteus cell from 1,3 x 109 cell / gr [at] fermentation first becomes 3,4 x 106 cell/gr and decrease after day fermentation second becoming 1,75 x 106 cell/gr. Proportion of increasing isoflavone content at day fermentation 1-2 from 19,1 becoming 52,7 mg/100gr. Inoculation of M. luteus bacteria increased to count isoflavone aglicone and no trouble at growth of tempe mold and it relate to the increasing of is full scale of yielded tempe isoflavone.
TINGKAT KEGANASAN KANKER SERVIKS PASIEN PRA-RADIASI MELALUI PEMERIKSAAN AgNORs, MIB-1 DAN Cas- 3 Iin Kurnia; Siti Harnina Bintari; Mafaza Khaisuntaha
Biosaintifika: Journal of Biology & Biology Education Vol 4, No 2 (2012): September 2012
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v4i2.2269

Abstract

Kanker serviks sering ditemukan di negara berkembang. Pengobatan kanker melalui radioterapi untuk mengetahui tingkat proliferasi dan mengurangi tingkat keganasan. Biomarker proliferasi dan apoptosis berupa AgNORs, MIB-1, dan Caspase 3. Namun belum dijelaskan mengenai korelasi ketiga biomarker dalam kaitannya dengan proliferasi dan apoptosis pada sel kanker serviks. Tujuan penelitian untuk mengetahui korelasi antara AgNORs, MIB-1, dan apoptosis pada kanker serviks. Penelitian observasional laboratoris menggunakan metode pewarnaan dengan menekankan kontras warna antara sitoplasma dan inti sel. Objek berupa sediaan mikroskopis dari 30 biopsi pasien kanker serviks. Pengambilan data dengan metode crocker dan blind manner. Analisis data menggunakan uji korelasi, dari ju mlah 21 pasien yang diamati menunjukkan. AgNORs dan MIB-1 memiliki angka relatif tinggi. Angka yang diperoleh ini berbanding terbalik dengan apoptosis yang relatif rendah. Korelasi antara AgNORs dengan MIB-1 menunjukkan r= 0,33 dan p= 0,15. AgNORs dengan apoptosis memiliki korelasi negatif yakni, r=-0,08 dan p= 0,73. MIB-1 dengan apoptosis memiliki korelasi negatif pula r= -0,18 dan p= 0,43. Kesimpulannya korelasi AgNORs dengan apoptosis memiliki kecenderungan lebih baik dari pada MIB-1 dengan apoptosis.Cervical cancer is often found in the developing countries. The treatment of cancer through radiotherapy was performed to determine the proliferation level and to reduce the malignancy level of cancer. The proliferation and apoptotic biomarkers were AgNORs, MIB-1, and Cas- 3. However, the correlation between the three biomarkers in relation to the proliferation and apoptosis in cervical cancer cells was not clear. The purpose of the study was to determine the correlation between AgNORs, MIB-1 and apoptosis in cervical cancer. This study was an observational research laboratory using a staining method to emphasize the color contrast between the cytoplasm and the nucleus of the cells. The microscopic preparations of the 30 patients with cervical cancer biopsies had been used as the study objects. Data was collected using the Crocker and Blind method and was then analyzed using correlation test. Data from 21 patients with AgNORs and MIB-1 showed a relatively high value. The figure obtained was inverse proportionally to the relatively low apoptotic value. The correlation between AgNORs and MIB-1 showed r = 0.33 and p = 0.15. There was negative correlation between AgNORs and apoptosis at r = -0.08 and p = 0.73. Meanwhile, between MIB-1 and apoptosis has also a negative correlation at r = -0.18 and p = 0.43. It was concluded that the correlation between AgNORs and apoptosis tended to be better than the MIB-1 and apoptosis.
The Growth of Beauveria bassiana on Corn Waste Medium Dyah Rini Indriyanti; Siti Harnina Bintari; Ning Setiati; Puput Roy Purnawati
International Conference on Science, Education, and Technology Vol. 8 (2022)
Publisher : Universitas Negeri Semarang

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Abstract

The purpose of this study was to analyze the density and viability of Beauveria bassiana grown on corn waste medium with 1% NPK (Nitrogen Phosphate Potassium) supplementation. This experimental study used a completely randomized design with 3 treatments and 9 repetitions. Each growth medium consisted of 25 grams of corn waste supplemented with NPK 1% with a dose of 0 mL (P0), 2mL (P1), and 3 mL (P2). The results of this study showed that the highest fungal density (16.72x108 conidia/mL) and viability (44.66%) were obtained from P2 treatment. It can be concluded that 1% NPK on corn waste medium affect the density and viability of Beauveria bassiana.
PENGEMBANGAN PRODUK TEMPE KELOR PADA UMKM SEBAGAI UPAYA PENGEMBANGAN WISATA DESA WILAYAH EMBUNG PATEMON GUNUNG PATI SEMARANG Siti Harnina Bintari; Wiwi Isnaini; Sri Sukaesih; Endah Peniati; Ely Rudyatmi
Prosiding Seminar Nasional Biologi Vol. 11 (2023)
Publisher : Prosiding Seminar Nasional Biologi

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Abstract

Wilayah Patemon mempunyai aset berupa Wisata Embung untuk tempat wisata masyarakat domestik. Upaya ini terus digalakkan guna memacu kunjungan masyarakat lokal dan wisata kuliner termasuk tempe. Tempe yang dipromosikan adalah tempe ori dan tempe kelor serta olahannya berupa keripik kelor tempe. Pembuatan tempe dilakukan oleh peserta kegiatan pengabdian untuk edukasi dan menjual produk hasil olahannya guna meningkatkan pendapatan dan bertambahnya pamor desa wisata Embung Patemon dengan melibatkan UMKM tempe Berkah NJ dan masyarakat setempat tergabung dalam komunitas kewirausahaan di kelurahan Patemon. Pengembangan produk tempe menjadi tempe kelor merupakan inovasi teknologi pengolahan pangan melalui fortifikasi tepung daun kelor, dan peserta memberi respon baik. Dari hasil observasi yang telah dilakukan penambahan tepung daun kelor konsentrasi 1% dengan lama fermentasi 36 jam menunjukkan prosentase flavonoid meningkat dan menunjukkan penurunan mulai umur fermentasi 48 jam. Selama proses pendampingan kegiatan pengembangan tempe kelor terjadi peningkatan pemasaran, penguatan kegiatan kewirausahaan dan terlaksana sosialisasi P-IRT serta penandanganan MoU antara dekan FMIPA dengan pihak kelurahan Patemon.
ANALISIS PENGARUH MOL TEMPE OVERFERMENTED TERHADAP KARAKTERISTIK KOMPOS: ASPEK FISIK, KIMIA, DAN BIOLOGI Siti Maesaroh; Siti Harnina Bintari
Prosiding Seminar Nasional Biologi Vol. 11 (2023)
Publisher : Prosiding Seminar Nasional Biologi

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Abstract

Penelitian ini mengeksplorasi pemanfaatan limbah pertanian, peternakan, dan industri, seperti jerami, kotoran kambing, dan tempe overfermented, dalam pembuatan kompos. Rancangan penelitian menggunakan Rancangan Acak Lengkap (RAL) dengan empat perlakuan (P1, P2, P3, P4). P1 menggunakan starter EM4, P2 MOL tempe overfermented, P3 EM4:MOL tempe overfermented 1:1, dan P4 EM4:MOL tempe overfermented 1:3, yang diulang enam kali, dengan pemeraman 21 hari. Analisis data melibatkan uji One Way ANOVA, dan analisis deskriptif. Hasil penelitian menunjukkan perubahan signifikan pada sifat fisik kompos, termasuk aroma, warna, tekstur, dan penyusutan, selama tiga minggu. Nilai pH dan suhu kompos juga berubah signifikan. Rasio C/N menurun secara berturut-turut pada P1, P2, P3, dan P4 yaitu 6,3; 6,0; 5,5; 5,0 dengan rasio C/N pada P0 (tanpa starter) sebesar 11,3. Analisis biologi menunjukkan P2 memiliki total bakteri tertinggi, 5,96 x 107, berasal dari bakteri asam laktat (BAL) dan Bacillus sp. Penelitian ini menyimpulkan bahwa pemanfaatan limbah tempe overfermented dan pertanian, peternakan, serta limbah industri dapat menjadi solusi inovatif untuk kompos organik. Hal ini mendukung upaya mengurangi limbah melalui daur ulang, membuka peluang pengembangan pupuk organik efektif dan berkelanjutan, dengan dampak positif pada pertanian dan lingkungan.
Co-Authors A.A. Ketut Agung Cahyawan W Alam, Aulia Nuanza Alamsyah - Alfiyan, Jamil Maulana Zahriyan Anam, M. Khairul Andin Vita Amalia, Andin Vita Anhari, Saeful Ani Nur Fauziah, Ani Nur Anik Ulfah, Anik Anisa Dyah P Anisa Dyah P Astrid Farmayati Caska - Chulia Mubtadiah, Chulia Danang Dwi Saputro Deddy Muchtadi DEDDY MUCHTADI Dewi Mustikaningtyas Dewi Susilowati Dhimas Fajar Eka Purnama Dwi Handayani DWI SUSILANINGSIH DWI YULIANTI Dyah Rini Indriyanti Eka - Mukaromah, Eka - Ely Rudyatmi, Ely Endah Peniati Enni Suwarsi Rahayu Evi Widowati Farikhul Ulum Farikhul Ulum Fazat Haniyya, Fazat Fernando, G. S.N. Fidia Fibriana Fitri Andiniyati Hadi Nasbey Hayati, Dwi Puji Hayati, Dwi Puji Heni Pujiastuti Ibnul - Mubarok, Ibnul - Ibnul Mubarok Ibnul Mubarok Ibnul Mubarok Ichsani, Nadya Iin Kurnia Iin Kurnia Intan Zainafree, Intan Isnaeni Karunia Annisa Istiani, Fahrun Istiani, Fahrun Jamil Maulana Zahriyan Alfiyan Jayanti, Septi Jumiyati - Jumiyati - Kartika Nugraheni Kartika Nugraheni Khairunisa, Nisrina L. Putri, Afra Fauziah Lail Isro, Abda Latipah, Rina April Lisdiana Lisdiana MADE ASTAWAN Mafaza Khaisuntaha Mafaza Khaisuntaha Maftuhah, Anis Margareta Rahayuningsih Nugroho Edi K Martien Herna Susanti Maryani Suwarno Maryani Suwarno Maryani Suwarno Masru'ah, Masru'ah Milah, Nihayatul Moeis, Siti Fatimah Muhammad Agus Muljanto Muhammad Khumaedi Mursyid . Mursyid Mursyid Mutiara Bintang Ramadani Nadya Ichsani Najah, Khoirun Nana Kariada Trimartuti Natalia Desy Putriningtyas, Natalia Desy Natalia, Lidya Ayu Nicky, Dhea Niken Subekti Nikmatul Hidayah Ning Setiati Ning Setiati Ning Setiati Nugraini, Anisa Ratna Nurika, Nurika P. Eko Prasetyo Parmin - Pramesti Dewi Pratama, Rizka Nur Priyantini Widiyaningrum Puput Roy Purnawati R. Susanti R. Susanti Rahina Nugrahani Rahmawati, Fahmi Retno - Istifarini, Retno - Retno Sri Iswari Rivana Citra r Rivana Citra R Rohman, Shohihatur Rosidah Rosidah Sadikin, Nadya Audina Nurkhafiya Saiful Ridlo Sarjadi Sarjadi Sarjadi Sarjadi Siti Alimah Siti Fatimah Moeis Siti Maesaroh Sri Sukaesih Sri Sukaesih Sri Utami Sri Widowati Sri Widowati Stephani Diah Pamelasari, Stephani Diah Sucihatiningsh Dian Wisika Prajanti, Sucihatiningsh Dian Wisika Sudarman Sudarman Sunyoto Sunyoto Sunyoto Sunyoto Syukron Rizqi, Syukron Talitha Widiatningrum Tiara Linanti, Annisa Tirtaningrum, Fitria Ayu Tri Budi Kurniawan Tri Budi Kurniawan, Tri Budi Tutik Wresdiyati Veronika Eka J Veronika Eka J Widya Hary Cahyati Widyanigrum, Dewi Widyanigrum, Dewi Winanti, Ruri Wiwi Isnaini Wulan Christijanti Wulandari K. E, Yenni Tyas Yanuar Revandi Yatin Mulyono Yuliana, Lusia Retno Yuliana, Lusia Retno Yuniarti Yuniarti Yustinus Ulung Anggraito