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KERAGAMAN GENETIK, KEBUGARAN DAN INKOMPATIBILITAS REPRODUKSI Hemiptarsenus varicornis Girault (Hymenoptera: Eulophidae), PARASITOID LARVA Liriomyza huidobrensis (Diptera: Agromyzidae) . Reflinaldon; Damayanti Buchori; Dwinardi Aprianto
Jurnal Hama dan Penyakit Tumbuhan Tropika Vol. 11 No. 1 (2011): MARET, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (292.801 KB) | DOI: 10.23960/j.hptt.1111-10

Abstract

Several experiments have been conducted to study genetic variation, fitness and reproductive incompatibility of H. varicornis from different geographic populations. Genetic variation from Pandai Sikek (PS), Alahan Panjang (AP) and Kayu Aro (KA) was analyzed by using random amplified polymorphic DNA-polymerase chain reaction (RAPD-PCR) technique and the similarity of genetics measured using NTSys program. The fitness of female wasps such as longevity, fecundity and preoviposition was observed and then compared among those populations. Incompatibility in reproduction was determined by accounting of reproductive compatibility (RC) index in crossing of intra and interpopulation both of PS and AP. The results showed high genetic variation of H. varicornis among population from Alahan Panjang, Pandai Sikek and Kayu Aro with similarity coefficient of 30 to 70%. The best fitness showed the female wasps from Kayu Aro that was significantly different (P= 0.00) in longevity (24.60 ± 6.4 days), fecundity (63.6 ± 28.6 eggs) and parasitization (53.60%) but not significantly different (P=0.07) in number of the first day eggs (1.1 ± 1.4 eggs). Crossing of AP and PS indicated incompatibility in reproduction among the population.
PENGARUH EKSTRAK RANTING AGLAIA ODORATA LOUR. TERHADAP PERKEMBANGAN PARASITOID ERIBORUS ARGENTEOPILOSUS (CAMERON) PADA INANG CROCIDOLOMIA BINOTALIS ZELLER Sudarmo , Djoko Prijono, Syafrida Manuwoto, dan Damayanti Buchori
Jurnal Hama dan Penyakit Tumbuhan Tropika Vol. 1 No. 2 (2001): SEPTEMBER, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (135.069 KB) | DOI: 10.23960/j.hptt.2163-70

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The effect of Aglaia odorata Lour. twig extract on development of a parasitic wasp Eriborus argenteopilosus (Cameron) in its host Crocidolomia binotalis Zeller. This laboratory study was conducted to evaluate the effect of ethyl acetate soluble fraction of methanolic twig extract of Aglaia odorata Lour. (Meliaceae) on the development of a parasitic wasp Eriborus argenteopilosus (Cameron) (Hymenoptera: Ichneumonidae) in its host larvae Crocidolomia binotalis Zeller (Lepidoptera: Pyralidae). Extract treatment was given through the host. C. binotalis larvae were fed broccoli leaves treated with A. odorata extract at LC25, LC50, and LC70, then were exposed to the female adults E. argenteopilosus. Mortality of the parasitized host larvae, survival of immature stages of the parasitoid in the host larvae, and longevity and fecundity of the emerging female parasitoids were recorded. The results showed that combinations of extract treatments (at LC25, LC50, and LC70) and parasitization increased the mortality of the parasitized host larvae (38,4 - 42,7%) more than twice that of the parasitized control larvae. Those extract treatments, however, did not exert any negative effects on developmental time of the parasitoid immature stages nor on some morphological characters, longevity and fecundity of the emerging female parasitoids. Thus, A. odorata extract showed promise to be integrated with the parasitoid for the control of C. binotalis.
Dinamika Populasi Kompleks Parasitoid Telur Helicoverpa armigera (Hubner) Pada Ekosistem Kapas Monokultur dan Kapas Tumpangsari dengan Jagung Damayanti BUCHORI; Bandung SAHARI; Dwi Adi SUNARTO
Jurnal Pengendalian Hayati Vol 1 No 2 (2008)
Publisher : Fakultas Pertanian Universitas Jember

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Abstract

Eggs of Helicoverpa armigera (Hübner) (Lepidoptera: Noctuidae) on its host plants could beparasitized by some species of parasitoids, especially of the genus Trichogrammatoidea andTrichogramma. This research was aimed to study the population dynamics of egg parasitoids of H.armigera on unsprayed fields of cotton monoculture and intercropped with maize. The plot size ofeach field was 19 m x 20 m, with five replicates. We collected H. armigera eggs from both fieldsperiodically since 23 days after planting (dap) to 100 dap. The eggs were collected on 9 sample units(size of sample unit: 1 m2) in 7-days interval. The number of collected and parasitized eggs wasrecorded and the emerged parasitoids were identified to species level. The result showed that thefluctuation pattern of H. armigera egg population was not different on cotton monoculture and cottonintercropped with maize. The parasitoid population was relatively high during the flowering phase ofcotton and maize. The parasitoids of H. armigera eggs found in cotton monoculture and cottonintercropped with maize were T’toidea armigera, T’toidea guamensis, T’toidea bactrae bactrae,T’toidea bactrae fumata, T. chilonis and T. chilotraeae . The dominant species on early season untilthe flowering phase of the plants was T’toidea armigera, while on the latter phase of the plantphenology was T. chilotraeae. Implication of this population dynamics and the succession of theparasitoids in biological control are discussed.
Does Landscape Complexity and Semi-Natural Habitat Structure Affect Diversity of Flower-Visiting Insects in Cucumber Fields? Akhmad Rizali; Damayanti Buchori; Susilawati Susilawati; Pudjianto Pudjianto; Yann Clough
AGRIVITA, Journal of Agricultural Science Vol 40, No 1 (2018): FEBRUARY
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v40i0.1722

Abstract

Presence of insects in agricultural habitat is affected by the surrounding circumstances such as the complexity and structure of landscape. Landscape structure is often formed as a consequence of the fragmentation of semi-natural habitat, which can negatively affect species richness and abundance of insects. This study was aimed to study the effect of complexity and structure of landscape on the diversity, abundance and traits of flower-visiting insects in cucumber fields. This study was conducted in cucumber fields surrounded by other agricultural crops, shrubs, semi-natural habitat and housing area, in Bogor, Cianjur and Sukabumi regencies, West Java, Indonesia. In a total of 16 agricultural areas, complexity and parameter of landscape especially class area (CA), number of patches (NumP), mean patch size (MPS), total edge (TE), and mean shape index (MSI) of seminatural habitats were measured. Sampling of flower-visiting insects was conducted using scan sampling methods. The result showed that landscape complexity affected species richness (but not abundance and trait) of flower-visiting insects both for mobile and less-mobile insects. Flower-visiting insects also responded differently to landscape structure. Species richness, abundance and variation of body size of mobile insects were affected by structure of semi-natural habitat.
Detection of Wolbachia Endosymbiont in Several Agriculturally Important Insect Parasitoids in Bogor, Indonesia Mahardika Gama Pradana; Giyanto Giyanto; Seiichi Furukawa; Satoshi Nakamura; Damayanti Buchori
AGRIVITA, Journal of Agricultural Science Vol 41, No 2 (2019)
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v41i2.1855

Abstract

Wolbachia that have been found to be maternally inherited in arthropods, including insects behave primarily as a reproductive parasite by inducing feminization and/or death among genetic males; causing cytoplasmic incompatibility, and activating parthenogenesis, in host insects. This research aims to detect the presence of Wolbachia in several parasitic insects and to determine the diversity of Wolbachia at the supergroup level. Insect genetic samples were collected, amplified using wsp (Wolbachia surface protein) gene and with the sequences then analyzed using species homologues drawn from the Genbank database. These activities were dedicated to detect intracellular presence of Wolbachia and assembled the phylogenetic tree of the respected bacteria. Phylogenetic analysis confirmed that Wolbachia existed in the samples belonged to the supergroup A (found in Cherapron sp.) and supergroup B (found in E. japonica). Wolbachia were detected in seven out of nine wasps i.e. Anagyrus lopezi, Ceraphron sp., Microplitis manilae, Brachymeria lasus, Scelionidae sp01, Trichogramma sp, and Exorista japonica. Single infection by Wolbachia supergroup A was detected in A. lopezi, Ceraphron sp., M. manilae, and Scelionidae sp01. Meanwhile single infection by Wolbachia supergroup B was detected only in E. japonica. Double infection by both supergroups occurred in B. lasus and Trichogramma sp. samples.
NGENGAT PARASITOID (Lepidoptera : Epipyropidae) PADA WERENG PUCUK METE DI PERTANAMAN JAMBU METE DI PULAU LOMBOK BAMBANG SUPENO; DAMAYANTI BUCHORI; PUDJIANTO PUDJIANTO; UTOMO KARTOSUWONDO; CHRISTIAN H. SCHULZE
Jurnal Penelitian Tanaman Industri Vol 15, No 1 (2009): Maret 2009
Publisher : Pusat Penelitian dan Pengembangan Perkebunan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jlittri.v15n1.2009.16-23

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ABSTRAKWereng pucuk mete, Sanurus indecora (Homoptera : Flatidae),merupakan hama utama tanaman jambu mete di pulau Lombok. Berbagaicara pengendalian telah dilakukan baik secara kimiawi maupun biologi.Pengendalian biologis yang telah banyak digunakan adalah pemanfaatandan eksplorasi musuh alami yang mencakup parasitoid, patogen danpredator. Salah satu musuh alami wereng daun (leafhoppers) dan werengpohon (planthoppers) yang masih belum diteliti di Indonesia dan baru 20spesies yang ada di di dunia adalah Epipyropidae. Ektoparasitoid familiEpipyropidae yang berasosiasi dengan imago S. indecora telah ditemukanpertama di Indonesia, khususnya di Pulau Lombok. Tujuan penelitian iniadalah untuk mengetahui keberadaan ngengat parasitoid pada inang S.indecora pada pertanaman jambu mete di lahan kering Pulau Lombok.Penelitian ini difokuskan pada dua kegiatan utama, yaitu pengambilansampel di lapang dan pengamatan laboratorium. Lokasi penelitian beradadi tiga desa di wilayah Kecamatan Gangga, Kayangan, dan Bayan.Penelitian dilakukan selama musim kemarau 2007 (Mei hingga Oktober2007) dan musim hujan (November 2007 hingga April 2008) di tiga kebunjambu mete milik petani yang ditanam secara monokultur. Dari penelitianini dapat diberikan beberapa kesimpulan, yaitu : wereng pucuk mete S.indecora berkembang baik pada musim kemarau (April hingga September)dan cenderung menurun pada saat musim hujan (Oktober hingga Maret),dengan populasi tinggi dicapai pada bulan Agustus hingga Oktober 2007.Larva ektoparasitoid Epypiropidae menyerang S. indecora jantan danbetina yang bersifat soliter dan atau gregarius. Laju parasitisasiEpipyropidae pada S. indecora jantan lebih kecil daripada betina, yaituberkisar 5,89 – 12,16% dan betina berkisar 15,23 – 19,23%. LarvaEpipyropidae tidak dapat menekan laju pertumbuhan populasi S. indecoradi pertanaman monokultur jambu mete di pulau Lombok. Denganperkataan lain bahwa semakin tinggi populasi S. indecora semakin rendahlaju parasitisasi yang ditemukan.Kata kunci : Anacardium occidentale, Sanurus indecora, ngengatparasitoid, EpipyropidaeABSTRACTParasitoid moth (Lepidoptera : Epipyropidae) on cashewplanthopper at cashew plantation in LombokSanurus indecora Jacobi is a serious pest attacking cashewplantation in Lombok Island. A number of natural enemies of flatids werefound on cashew plantation such as predator, pathogen, and parasitoid. Allmembers  of  Epipyropidae  (Lepidoptera)  are  ectoparasitoid  onplanthoppers and leafhoppers (Homoptera). The first report onEpipyropidae in Indonesia was documented, in Lombok whereEpipyropidae parasitized S. indecora. Study was conducted to determinethe prevalence of parasitoid moth on S. indecora at Lombok uplandcashew plantations. This experiment was conducted on May 2007 untilApril 2008 in three village areas of Gangga, Kayangan, and Bayandistricts. The results showed that population of Sanurus indecora increasesgradually from April until October (dry season) and decreases fromNovember until March (rainy season), with the highest population occursin August to October. Epipyropidae attacks both male and female of S.indecora J. Parasitation rate of male ranges from 0.38 – 46.00% with anaverage of 8.96%. Parasitation rate of female varies from 8.77 - 38.52%with an average of 17.45%. Epipyropidae is a solitary and or gregariousparasitoid. The parasitation rate was negatively correlated with S. indecorapopulation. The numbers of Epipyropidae larvae were correlated with thenumbers of S. indecora infected.Key words : Anacardium occidentale, Sanurus indecora, parasitoid moth,Epipyropidae
Pengaruh cendawan endofit terhadap biologi dan statistik demografi wereng batang cokelat Nilaparvata lugens Stál (Hemiptera: Delphacidae) Amanda Mawan; Damayanti Buchori; Hermanu Triwidodo
Jurnal Entomologi Indonesia Vol 12 No 1 (2015): Maret
Publisher : Perhimpunan Entomologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (440.956 KB) | DOI: 10.5994/jei.12.1.11

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Endophytic fungi is an endosymbiont that lives within host plant tissues and does not necessary cause any harm to plants. This type of fungus are important as mediators in plant-herbivore interactions. One of the endophytic fungi in rice is Nigrospora sp. The effects of Nigrospora sp. on the biology and demographic statistic of Nilaparvata lugens Stál (Hemiptera: Delphacidae) were studied in the laboratory. We used Nigrospora sp. culture powder was used to inoculate the fungi to rice seeds by mixing 10 g of flour endophytic per 1 kg rice seeds. The mixture was then stored in damp and dark storage. Results showed that the rice seeds treated with endophytic fungi showed some resistance to N. lugens. Eggs and early stages of nymph mortality was increased, higher than the control. Endophytic fungi also affect the nymphs growth rates by slowing it down, prolonging N. lugens life cycle, preoviposition period as well as delayed the age at first reproduction. N. lugens population growth is effected by Nigrospora sp. in laboratory scale. Thus, it has the potential as an alternative way to control N. lugens population. In addition, inoculation of endophytic fungi could be a useful method for protecting rice plants from N. lugens.
Keanekaragaman dan persebaran lalat buah Tribe Dacini (Diptera: Tephritidae) di Kabupaten Bogor dan sekitarnya Anik Larasati; Purnama Hidayat; Damayanti Buchori
Jurnal Entomologi Indonesia Vol 10 No 2 (2013): September
Publisher : Perhimpunan Entomologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (911.056 KB) | DOI: 10.5994/jei.10.2.51

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Bogor and its surrounding area is known as one of the region in West Java that has high diversity of horticultural plants that may have an affect on fruit fly diversity. Research on fruit fly diversity is not merely provide information on the species richness of fruit flys but also provide information on its distribution and dispersion. The aim of this research was to investigate the diversity and distribution of fruit flies species and their hosts in Bogor and its surrounding area. Fruit flies were collected from 119 sampling areas in Bogor Cianjur, Bekasi and Depok. Fruit flies were sampled using two methods, i.e. host rearing and trapping. Traps were modified from Lynfield traps and combined with two different attractants, i.e. metil eugenol (ME) and Cue lure (CL). We found 18 species of fruit flies collected from traps and 24 host plants. The result showed that distribution, diversity and abundance of fruit flies was influenced by the diversity of host plants.
Teknik perbanyakan massal parasitoid Anagrus nilaparvatae (Pang et Wang) (Hymenoptera: Mymaridae) dengan kotak plastik Araz Meilin; Y. Andi Trisyono; Edhi Martono; Damayanti Buchori
Jurnal Entomologi Indonesia Vol 9 No 1 (2012): April
Publisher : Perhimpunan Entomologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (435.883 KB) | DOI: 10.5994/jei.9.1.7

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Anagrus nilaparvatae (Pang et Wang) is an egg-parasitoid of rice brown planthopper (Nilaparvata lugens Stål). The objective of this study was to develop a mass-rearing technique of A. nilaparvatae. Mass-rearing was done using a plastic box (14 cm × 18.5 cm × 18.5 cm, made of plastic-mica, the upper side equipped with a 10 cm test tube), placed on a tray with rice seedlings containing an estimated of 11,130 eggs of N. lugens. About 355 adult parasitoids were successfully released per box when each box was infested with 50 parasitoids. The number of parasitoids produced were reduced to 164 adults when the infestation was made at 100 parasitoids per box. The emergence of parasitoids started around 07:00 am until 04:00 pm with the highest rate (36.2%) occured at 10:00 am. One plastic box cost only Rp10.000,-; thus the estimate cost to produce one parasitoid using this tecnique was Rp60,- These findings suggest that the plastik box is a simple and inexpensive technique for mass-rearing of A. nilaparvatae.
Pengaruh lama ketiadaan inang terhadap kapasitas reproduksi parasitoid Snellenius manilae Ashmead (Hymenoptera: Braconidae) Mohamad Eldiary Akbar; Damayanti Buchori
Jurnal Entomologi Indonesia Vol 9 No 1 (2012): April
Publisher : Perhimpunan Entomologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (380.491 KB) | DOI: 10.5994/jei.9.1.14

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The objective of this research was to study the  influence  of host deprivation on the oviposition and physiological condition of Snellenius manilae Ashmead. The research was conducted at Laboratory of Bioecology of Parasitoid and Predator, Department of Plant Protection, Faculty of Agriculture, IPB. Ten parasitoids of the same age and cohort were used in this experiment. Deprivation of hosts were done for 1, 2, 3, 4, 5, 6 and 7 days at the beginning and toward the end of their life. All hosts were replaced every 24 hours. Result indicated that host deprivation affects the reproductive capacity of Snellenius manilae. Even though parasitoids were able to lay eggs even when they were deprived of hosts for seven consecutive days, the overall results of host deprivation experiment showed that the length and timing of deprivation period can affect reproductive capacity. Deprivation of hosts tends to increase the parasitism rate and the numbers of eggs laid upon their first encounter of hosts after the treatment. The difference is more pronounced on treatements that allowed parasitism to occur before the deprivation treatment. However, the overall results suggests that deprivation overall decrease the reproductive capacity of the parasitoid. The longevity of deprived individuals was also lengthened. Parasitoids that were given host before deprivation treatments tend to produced more eggs than those were not. These results showed that deprivation of hosts in the field may affect the effectiveness of parasitoids and the success of biological control.
Co-Authors . Giyanto . Reflinaldon Abdilah, Nurullah Asep Abdul Munif Abdul Rahim Adha Sari Adha Sari Agus Wahyana Anggara Akhmad Rizali Akhmad Rizali Akhmad Rizali ALI NURMANSYAH Amanda Mawan Amrulloh, Rosyid Anik Larasati Anik Larasati Anis Rohmani Apriyadi, Rion Araz Meilin Araz Meilin Araz Meilin Armowidi, Tri Asep Saepuddin Azhar, Azru B T Rahardjo BAHAGIAWATI AMIR HUSIN Bambang Supeno Bambang Supeno Bambang Supeno, Bambang Bambang Suryobroto Bandung Sahari Bandung Sahari BANDUNG SAHARI Bandung Sahari Bonjok Istiaji Bramasto Nugroho Budi Susetyo Choirul Mahdianto CHRISTIAN H. SCHULZE CHRISTIAN HANSJOACHIM SCHULZE Dadan Hindayana Dede Maryana DEWI SARTIAMI DJOKO PRIJONO DWI ADI SUNARTO Dwinardi Aprianto DWINITA W UTAMI Edhi Martono Endang Sri Ratna Endang Sri Ratna ENRI AGUS SETIANI Erna Dwi Herawati Evawaty S. Ulina, Evawaty S. Fahmi, Fajrin Fatimah Siddikah Fatimah Siddikah Fitta Setiajiati Gilang Aditya Rahayu Hagus Tarno Harianto, Muhammad Hasmiandy Hamid Hasriyanty Hasriyanty Heri Tabadepu Hermanu Triwidodo Husni dan Zulfanazli Pudjianto Alfian Rusdy I WAYAN SUANA I Wayan Winasa Idham Sakti Harahap Ihsan Nurkomar Ihsan Nurkomar Inez H. Slamet -Loedin Iriyani, Silfi Johannis, Midzon Kasmiatun Kasmiatun Kasmiatun Kusumah, R Yayi Munara LILIK BUDIPRASETYO Lizmah, Sumeinika Fitria Loedin, Inez H. Slamet Luna Lukvitasari Lutfi Afifah Mahardika Gama Pradana Manuwoto, Syafrida Martina Martina Marwoto . Masy'ud, Burhanuddin MAWAN, AMANDA MELTA R. FAHMI MERIJN MARINUS BOS Mihwan Sataral MOERFIAH . Mohamad Eldiary Akbar Muhamad Nurhuda Nugraha Muhammad Arman Muhammad Badrus Sholih Muhammad Iqbal Tawakkal Murtiyarini Murtiyarini N. Usyati Najmi, Lailatun Nely Zubaedah Nina Herlina NOVRI NELLY Novriyanti NURARIATY AGUS NURINDAH NURINDAH Nurkomar, Ihsan Nurul Novianti Puspitaningtyas Nurulalia, Lia Permatasari, Gusthi Ayu PURNAMA HIDAYAT RACHMAWATI RACHMAWATI RAHMAT SYAHNI Ramadhani Eka Putra RIKA RAFFIUDIN Rinekso Soekmadi Rizky Nazarreta Rizky Nazarreta Rizky Nazarreta, Rizky Roni Koneri Rosyid Amrulloh Rudi Tarumingkeng S. Tjitrosemito Safitri, Betari Sapdi Sapdi Sapdi Sapdi SARI, ADHA Satoshi Nakamura SAURIN HEM Seiichi Furukawa SEIKI YAMANE Septiantina Dyah Riendriasari Setiajiati , Fitta Sih Kahono SJAFRIDA MANUWOTO SJAFRIDA MANUWOTO Sri Heriza Sri Ningsih Desi Afriany Sulthoni, Fahmi Suryo Wiyono Susilawati Susilawati Susilawati Susilawati Swastiko Priyambodo, Swastiko SYAFRIDA MANUWOTO Syafrida Manuwoto TARUNI SRI PRAWAST MIEN KAOMINI ANY ARYANI DEDY DURYADI SOLIHIN Tawakkal, Muhammad Iqbal Tazkiyatul Syahidah TEGUH SANTOSO Tri Atmowidi TRIMURTI HABAZAR Usyati, N. UTOMO KARTOSUWONDO UTOMO KARTOSUWONDO Van Basten Tambunan WINDRA PRIAWANDIPUTRA, WINDRA Woro Anggraitoningsih Noerdjito Woro Anggraitoningsih Noerdjito Y. Andi Trisyono YAHER WANDI Yann Clough YULIARTI, NETTI