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Modifications in Adenoviral vectors to enhance its tropism: a literature review Erly Sintya; Ni Luh Putu Harta Wedari; Ni Nyoman Sri Budayanti; Ni Luh Made Mirah Rahayu
Journal of Clinical Microbiology and Infectious Diseases Vol. 2 No. 2 (2022): Available Online: December 2022
Publisher : Indonesian Society for Clinical Microbiology (Perhimpunan Dokter Spesialis Mikrobiologi Klinik Indonesia)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51559/jcmid.v2i2.32

Abstract

Viruses’ high rate of cellular entry raises the possibility that they could be utilized as vectors to introduce new functional copies of a gene into a cell. This review aims to explore modifications in Adenoviral vectors to enhance its tropism. It is feasible to exploit the infection pathway to achieve a therapeutic objective without the following expression of viral genes, which, if expressed, would cause disease and damage. It has been demonstrated that this is achievable. This is performed by swapping a therapeutic gene with an existing harmful gene in the genome of the virus. Due to its unique features, adenovirus, commonly known as Ad, is an intriguing possibility for use as a viral vector in gene therapy. Despite this, therapeutic applications of ad vectors are limited due to their immunogenicity and broad native tropism. Several distinct forms of nonimmunogenic polymers are utilized in the chemical or physical modification of ad vectors to circumvent these obstacles. In this review, many modifications, including capsid pseudotyping, serotype switching, and multiple conjugation-based techniques, are discussed in order to boost the specificity of target adenoviruses.
Karakteristik Bakteri Asam Laktat pada Feses Luwak di Beberapa Wilayah Pulau Bali Winatha, I Gde Pangestu Putrama; Hendrayana, Made Agus; Sukrama, I Dewa Made; Budayanti, Ni Nyoman Sri
E-Jurnal Medika Udayana Vol 11 No 7 (2022): E-Jurnal Medika Udayana
Publisher : Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/MU.2022.V11.i7.P11

Abstract

Kopi luwak merupakan kopi yang difermentasi pada saluran pencernaan luwak yang dapat memberikan cita rasa yang khas pada kopi tersebut. Pada proses saluran pencernaan hewan luwak terdapat bakteri yang memegang pernan penting dalam proses fermentasi biji kopi, yaitu bakteri asam laktat. Penelitian ini bertujuan untuk mengetahui karakteristik bakteri asam laktat pada feses luwak yang memegang peranan penting dalam saluran pencernaan hewan luwak. Penelitian ini adalah penelitian deskriptif dengan rancangan penelitian observasional. Penelitian ini adalah penelitian deskriptif dengan rancangan penelitian observasional. Pada penelitian ini digunakan sebanyak 18 sampel yang diambil dari agrowisata di setiap kabupaten di Pulau Bali. Seluruh sampel diperiksa di Laboratorium Mikrobiologi Fakultas Kedokteran Universitas Udayana dan Laboratorium Kesehatan Provisinsi Bali. Sampel dikultur pada media MRS-CaCo3 dan hasil positif bakteri asam laktat akan dilakukan uji gas CO2, uji pH, uji katalase, uji pewarnaan gram, serta diidentfikasi menggunakan metode semi automatic BD BBL Crsytal. Dari 18 sampel feses luwak yang dikultur ditemukan 16 diantaranya merupakan bakteri gram positif dan berkatalase negatif yang merupakan ciri ciri bakteri asam laktat. Pada uji gas CO2 ditemukan 9 koloni bakteri yang bersifat homofermentatif, dan 7 bersifat heterofermentatif. Pada uji pH keseluruhan koloni bakteri berada pasa suasana asam dengan rentang pH 4-5. Pada pengamatan di bawah mikroskop ditemukan bentuk bakteri bulat dan batang. Kemudian terdapat beberapa spesies yang ditemukan, yaitu: Corynebacterium Genitalium, Kytococcus sedentarius, Streptococcus sanguinis, dan Streptococcus faecium. Pada penelitian ini ditemukan beberapa karakteristik bakteri asam laktat yaitu bakteri gram positif dengan katalase negatif, memiliki suasana pH yang asam, memiliki bentuk bervariasi dari batang hingga bulat, serta dapat bersifat heterofermentatif atau homofermentatif. Kata Kunci : Kopi luwak, Bakteri asam laktat, Karakteristik
IDENTIFIKASI BAKTERI Methicillin-Resistant Staphylococcus aureus PADA TELEPON GENGGAM MAHASISWA PSSKPD 2019 FAKULTAS KEDOKTRERAN UNIVERSITAS UDAYANA Hendrawan, Gresya; Sukrama, I Dewa Made; Budayanti, Ni Nyoman Sri; Hendrayana, Made Agus
E-Jurnal Medika Udayana Vol 12 No 7 (2023): E-Jurnal Medika Udayana
Publisher : Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/MU.2023.V12.i07.P12

Abstract

Latar belakang: Kebersihan dari telepon genggam kerap dilupakan oleh penggunanya, tidak terkecuali oleh mahasiswa. Hal ini menyebabkan telepon genggam dapat beresiko menjadi tempat hidup berbagai bakteri, salah satunya adalah Staphylococcus aureus. Beberapa strain S. aureus memiliki kemampuan bertahan terhadap antibiotik metisilin atau yang disebut Methicillin-resistant Staphylococcus aureus (MRSA). Adanya bakteri resisten dapat menyebabkan berbagai infeksi serius sehingga lebih sulit untuk disebuhkan dan membutuhkan biaya pengobatan yang tinggi. Maka dari itu skrining sangat penting dilakukan untuk meningkatkan kewaspadaan agar dapat mencegah kontaminasi dan infeksinya. Tujuan: Penelitian ini bertujuan untuk mengetahui persentase mahasiswa PSSKPD 2019 Fakultas Kedokteran Universitas Udayana yang telepon genggamnya teridentifikasi bakteri S. aureus dan MRSA. Metode: Penelitian adalah observasional dengan pendekatan potong lintang deskriptif. Sampel penelitian dipilih secara acak dengan teknik simple random sampling. Pengambilan sampel dilakukan dengan melakukan swab pada layar telepon genggam dengan menggunakan lidi kapas steril. Spesimen kemudian ditumbuhkan pada media Mannitol Salt Agar. Koloni yang terbentuk diuji dengan menggunakan metode katalase, koagulase dan pengecatan gram. Sampel yang teridentifikasi positif S. aureus kemudian diperiksa kemampuan resistensinya terhadap antibiotik dengan menggunakan disk cefoxitin 30?g. Hasil: Penelitian dilakukan pada 37 sampel telepon genggam milik mahasiswa PSSKPD angkatan 2019 FK Unud yang diperiksa untuk mengidentifikasi bakteri S. aureus dan MRSA. Ditemukan sebanyak 4 sampel atau sebesar 10,81% positif S. aureus. Dari total isolat S. aureus, 50% merupakan bakteri MRSA. Simpulan: Terdapat kontaminasi bakteri S. aureus pada telepon genggam milik mahasiswa kedokteran yang cukup tinggi. Di antara bakteri tersebut yang terdapat bakteri yang resisten terhadap antibiotik yaitu MRSA.
IDENTIFIKASI BAKTERI Methicillin-Resistant Staphylococcus aureus PADA PERMUKAAN BENDA DI POLIKLINIK RAWAT JALAN RUMAH SAKIT UNIVERSITAS UDAYANA Saranova, Hilda; Hendrayana, Made Agus; Sukrama, I Dewa Made; Budayanti, Ni Nyoman Sri
E-Jurnal Medika Udayana Vol 13 No 9 (2024): E-Jurnal Medika Udayana
Publisher : Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/MU.2024.V13.i09.P02

Abstract

Peralatan medis yang digunakan secara bersamaan tanpa disinfeksi secara memadai dapat memudahkan transmisi mikroorganisme penyebab infeksi nosokomial, khususnya Staphylococcus aureus. Pencegahan infeksi nosokomial dapat dilakukan dengan mengoptimalkan peran pelayanan kesehatan salah satunya dengan melakukan pengamatan mengenai gambaran dan resistensi bakteri terhadap antibiotika. Tujuan penelitian ini untuk mengetahui jumlah MRSA yang menjadi reservoir alat medis pada lingkungan Poliklinik Rawat Jalan dan Instalasi Gawat Darurat Rumah Sakit Universitas Udayana. Penelitian ini menggunakan desain deskriptif observasional potong-lintang dengan populasi terjangkau berupa peralatan medis stetoskop, termometer, termogun, dan tensimeter dengan frekuensi penggunaan yang tinggi. Sampel diambil menggunakan metode total sampling dengan tetap memperhatikan kriteria inklusi dan eksklusi. Sampel diambil menggunakan metode swab yang kemudian dikultur pada media MSA. Uji katalase dan koagulase dilakukan untuk koloni yang dicurigai Staphylococcus aureus dan dilanjutkan dengan pewarnaan Gram. Isolat kemudian dilakukan pengujian sensitivitas antibiotik menggunakan cefoxitin 30 ?g. Dari total 28 sampel, tidak ditemukan MRSA pada peralatan medis. Prevalensi alat medis yang menjadi reservoir bakteri S.aureus secara keseluruhan adalah 10,71%. Prevalensi tertinggi berdasarkan lokasi terdapat pada poliklinik anak (7,14%) dan ruangan screening poliklinik (3,57%). Ketiga sampel S.aureus yang didapatkan menunjukkan hasil sensitif yang menandakan bahwa bakteri tersebut merupakan Methicillin Sensitive Staphylococcus aureus (MSSA).
KOLONISASI BAKTERI METHICILLIN-RESISTANT STAPHYLOCOCCUS AUREUS (MRSA) PADA UANG KERTAS YANG BEREDAR DI KANTIN FAKULTAS KEDOKTERAN UNIVERSITAS UDAYANA Chandra, Katherine Silvania; Mayura, I Putu Bayu; Budayanti, Ni Nyoman Sri; Hendrayana, Made Agus
E-Jurnal Medika Udayana Vol 13 No 5 (2024): E-Jurnal Medika Udayana
Publisher : Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/MU.2024.V13.i05.P15

Abstract

Money serves as a widely utilized medium of transactional value in society. The risk of infection resulting from bacterial dissemination on paper currency is notably high. Infections caused by microorganisms on paper currency are largely caused by bacteria that resist antibiotics, exemplified by Methicillin-Resistant Staphylococcus aureus (MRSA). The resistance exhibited by these bacteria to antibiotic treatments has claimed millions of lives and can lead to substantial global health repercussions. Therefore, this study aims to ascertain the presence of Methicillin-Resistant Staphylococcus aureus (MRSA) bacteria contaminating paper currency. This research was conducted with observational methodology and a descriptive cross-sectional approach. Thirty samples of paper currency are swabbed, and specimens are subsequently inoculated onto Staphylococcus medium. Colonies of Staphylococcus are re-identified using gram staining, catalase tests, followed by coagulase tests to ascertain the presence of Staphylococcus aureus bacteria. Isolates are classified as Methicillin-Resistant Staphylococcus aureus (MRSA) if the inhibition zone measures less than or equal to 21 mm. The research findings indicate the colonization of Staphylococcus aureus and Methicillin-Resistant Staphylococcus aureus (MRSA) bacteria on the paper currency circulating in the canteen of the Medical Faculty at Udayana University. The percentage of Staphylococcus aureus colonization is 56.66% and Methicillin-Resistant Staphylococcus aureus (MRSA) colonization is 53.33%. Keywords : Paper Currency, Methicillin-Resistant Staphylococcus aureus(MRSA).
IDENTIFIKASI SUBTIPE Enterotoxigenic Escherichia coli DAN Enteroaggregative Escherichia coli PADA BIJI KOPI FESES LUWAK (Paradoxorus hermaphroditus) DARI BEBERAPA AGROWISATA DI BALI Wira Guna, I Gede Bhima; Budayanti, Ni Nyoman Sri; Hendrayana, Made Agus; Sukrama, I Dewa Made
E-Jurnal Medika Udayana Vol 12 No 9 (2023): E-Jurnal Medika Udayana
Publisher : Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/MU.2023.V12.i09.P15

Abstract

ABSTRAK Latar Belakang: Kopi luwak merupakan salah satu kopi yang terkenal akan citra rasanya. Indonesia merupakan salah satu penghasil kopi luwak terbaik di dunia, Bali merupakan salah satu tempat yang banyak memproduksi kopi luwak. Kopi luwak berasal dari hasil pencernaan hewan luwak yang memakan biji kopi. Luwak merupakan hewan berdarah panas yang didalam sistem pencernaannya terdapat banyak bakteri flora nomal, yaitu Escherichia coli. Pada proses pengolahan dari biji kopi feses luwak masih banyak dilakukan secara tradisional, hal tersebut membuat petani luwak yang mengolah berpeluang tercemar bakteri Escherichia coli sehingga dapat menyebabkan diare. Penelitian ini bertujuan untuk mengdentifikasi adanya bakteri Escherichia coli subtipe Enterotoxigenic Escherichia coli (ETEC) dan Enteroaggergative Escherichia coli (EAEC) pada biji kopi feses luwak dari beberapa agrowisata di Bali. Metode: Penelitian ini adalah penelitian deskriptif dengan rancangan penelitian observasional. Penelitian ini menggunakan sebanyak 18 sampel yang diambil pada agrowisata di setiap kabupaten dan kota di Bali. Seluruh sampel diperiksa di Laboratorium Mikrobiologi Fakultas Kedokteran Universitas Udayana. Sampel tersebut dikultur pada media Eosin Methylen Blue Agar (EMBA) dan hasil murni bakteri Escherichia coli dilanjutkan uji konfrontasi menggunakan pengecatan gram, serta tahap identifikasi subtipe ETEC dan EAEC dengan menggunakan metode Polymerase Chain Reaction (PCR). Hasil: Dari 18 sampel yang dikumpulkan, 13 (72%) sampel positif bakteri Escherichia coli. 13 sampel positif dilanjutkan ke proses PCR dan didapatkan tidak terdeteksi adanya subtipe ETEC dan EAEC. Simpulan: Pada penelitian ini ditemukan bakteri Escherichia coli pada Sebagian besar sampel biji kopi feses luwak yaitu 72%. Namun setelah diidentidikasi lebih lanjut pada metode PCR, tidak adanya subtipe ETEC dan EAEC pada seluruh sampel positif Escherichia coli. Kata Kunci : Biji kopi feses luwak, Escherichia coli, Diare
IDENTIFIKASI BAKTERI Escherichia coli SUBTIPE Enteroaggregative Escherichia coli DAN Enteropathogenic Escherichia coli DENGAN METODE KULTUR DAN POLYMERASE CHAIN REACTION PADA SATE DAGING BABI DI KOTA DENPASAR Putra, I Dw. Gd. Bayu Artha Pratama; Sukrama, I Dewa Made; Budayanti, Ni Nyoman Sri; Hendrayana, Made Agus
E-Jurnal Medika Udayana Vol 11 No 5 (2022): E-Jurnal Medika Udayana
Publisher : Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/MU.2022.V11.i05.P13

Abstract

ABSTRACT Pork satay is a traditional Balinese dish whose basic ingredient is pork. Pork contains Escherichia coli bacteria which is a normal flora in pigs, so if it is not processed properly and hygienically, it has the potential to be contaminated with Escherichia coli bacteria from pork or in the processing process which can cause diarrhea if consumed. This study aims to identify the presence of Escherichia coli bacteria contamination as well EAEC and EPEC subtypes on pork satay in Denpasar City. This research is a descriptive study with an observational research design. In this study, using cluster purposive sampling technique with 16 samples taken from each sub-district in Denpasar City. All samples were examined at the Microbiology Laboratory, Faculty of Medicine, Udayana University. The samples were cultured on Eosin Methylene Blue Agar (EMBA) media and the positive results of Escherichia coli bacteria from the culture process were continued to the identification stage using the Polymerase Chain Reaction (PCR) method. Of the 16 samples of pork satay tested for culture, 9 (56%) samples were positive for Escherichia coli bacteria. All positive samples were continued with the identification process using the Polymerase Chain Reaction (PCR) method and the results showed no one of EAEC and EPEC subtypes were detected in all positive samples. In this study, most of the pork satay samples were contaminated with Escherichia coli bacteria, but after molecular identification, no one of EAEC and EPEC subtypes were found in all samples that were positive for Escherichia coli bacteria. Keywords : Pork satay, Escherichia coli, Diarrhea
UJI DAYA HAMBAT EKSTRAK ETANOL BASE GEDE TERHADAP PERTUMBUHAN BAKTERI Escherichia coli Wibisana, I Dewa Nyoman Adi Ningrat; Hendrayana, Made Agus; Sukrama, I Dewa Made; Budayanti, Ni Nyoman Sri
E-Jurnal Medika Udayana Vol 13 No 9 (2024): E-Jurnal Medika Udayana
Publisher : Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/MU.2024.V13.i09.P01

Abstract

Foodborne disease is a condition in which food is contaminated by microorganisms or chemicals, causing disease. One of the microorganisms that most often causes this condition is Escherichia coli. There is an assumption which states that, base gede/base genep has antibacterial properties which inhibit the growth of bacteria in typical Balinese food such as lawar. Various studies have proven that the basic ingredients of base gede have antibacterial activity obtained from secondary metabolites. The purpose of this study was to determine the phytochemical compounds contained in the base gede and the inhibitory power of the base gede in inhibiting the growth of the Escherichia coli ATCC 25922. This study examined the content of secondary metabolites qualitatively and the experimental test was the true experimental posttest only control group design. The method used was the disk diffusion (Kirby Bauer) with variations in the concentration of ethanol extract base gede as many as four groups (25%, 50%, 75% and 100%) and the negative control group was 96% ethanol and the positive control group ciprofloxacin 5g. The results of the phytochemical test found that the base gede ethanol extract contained phenols, saponins, tannins, alkaloids, terpenoids, and flavonoids. Inhibition test results observed inhibition zones formed at concentrations of 50%, 75%, 100% and K+ with diameters respectively 5.65+/-0.65 mm ; 7.00+/-1.35mm ; 7.78+/-1.18 mm dan 34.52+/-1.49 mm. Kruskal wallis test showed a value of p = 0.001 (p <0.05) which showed that the concentration group had an effect on the inhibition of E.coli ATCC 25922. There was a significant difference between the 50% and 100% concentrations obtained from the Mann-Whitney analysis with value of p = 0.043 (p<0.05). Keywords : Inhibition test, base gede, Escherichia coli
Antimicrobial Resistance Profile of MDR & Non-MDR Meropenem-Resistant Pseudomonas aeruginosa Isolates of Patients in Intensive Care Unit of Tertiary Hospital Lameng, Imaculata Sonia Vidaryo; Budayanti, Ni Nyoman Sri; Prilandari, Luh Inta; Adhiputra, I Ketut Agus Indra
Indonesian Journal of Tropical and Infectious Disease Vol. 9 No. 3 (2021)
Publisher : Institute of Topical Disease Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ijtid.v9i3.30000

Abstract

Pseudomonas aeruginosa is one of the gram-negative bacteria that causes infection in the Intensive Care Unit (ICU) which is easily resistant. Patients infected with carbapenem-resistant P. aeruginosa are predicted to have a poor prognosis. This study aims to know the resistance profile of meropenem-resistant P. aeruginosa in the ICU. The results of this study can be used as a measure on the success of antimicrobial resistance control, infection control programs and become a reference for empirical therapy in the ICU. This study used a cross-sectional retrospective descriptive research method and was carried out at the Clinical Microbiology Laboratory of Sanglah Hospital Denpasar for three years, from 2018 to 2020. The results showed 38 of the 93 isolates of P. aeruginosa in the ICU were resistant to meropenem and were derived from sputum and urine. The percentage of meropenem-resistant P. aeruginosa isolates was higher in the multi-drug-resistant group and mostly came from sputum specimens. In 2018, Non-MDR meropenem-resistant P. aeruginosa isolates was that 100% sensitive to all other antibiotics used to treat P. aeruginosa infections, including; ceftazidime, cefepime, ciprofloxacin, gentamicin, amikacin, and piperacillin-tazobactam. In 2019 no meropenem-resistant P. aeruginosa isolates were found. In 2020, its sensitivity to antibiotics ceftazidime and piperacillin-tazobactam was 20.0%, ciprofloxacin 60.0% and to antibiotics gentamicin and amikacin 100%. MDR meropenem-resistant P. aeruginosa isolates in 2018 were still sensitive to ceftazidime (15.4%) and amikacin (69.2%) antibiotics, while in 2019 they were only sensitive to amikacin (37.5%). In 2020, P. aeruginosa isolates were sensitive to the antibiotics ceftazidime and cefepime (11.1%), piperacillin-tazobactam (22.2%), and amikacin (88.9%). Amikacin may be the choice of treatment for MDR meropenem-resistant P. aeruginosa.
Clostridium difficile Infection (CDI) by Hypervirulent BI/NAP1/027 Strain: a Comprehensive Review of Toxigenicity, Pathogenesis, Risk Factors, and Preventative Measures Budayanti, Ni Nyoman Sri; Aryana, I Gusti Putu Suka; Wedari, Ni Luh Putu Harta
Indonesian Journal of Tropical and Infectious Disease Vol. 10 No. 1 (2022)
Publisher : Institute of Topical Disease Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ijtid.v10i1.31030

Abstract

Clostridium difficile is an anaerobic bacil gram-positive bacteria, able to form spores and toxin, that is transmitted among humans through the fecal–oral route. Clostridium difficile infection (CDI), a typical nosocomial infection has been contributed to a signifi cant proportion of morbidity and mortality among in-patients with a case-fatality rate of 14% within 30 days after diagnosis. Profound culture and toxin examination for C. difficile are still minimal in many hospitals in various Asian countries. Consequently, C. difficile reports in Asia remain rare. Highly virulent form of C. difficile caused greater fatality and epidemics severity. Elderly age, hospitalization, exposure to antibiotics e.g., cephalosporins, fluoroquinolones, clindamycin, and penicillin contributed as main risk factors. Hypervirulent strain BI/NAP1/027 demonstrated to carry CdtLoc gene locus encodes CD196 ADP-ribosyltransferase (CDT) or known as binary toxin. Virulence factors are TcdA, TcdB, CDTa CDTb in which hypersporulation and mutation of TcD gene by hypervirulent strain led to toxin hyperexpression. Early cases detection, building management team to evaluate patient positive with all C. difficile toxins, hand hygiene improvement, continuation of contact precautions after diarrhea resolution, audit of infection control, and restriction of antimicrobials should be implemented as preventative measures. Focus measures also should emphasize on development of vaccine of C. difficile to boost immune state of elderly people. This review aims to describe severity of disease caused by hypervirulent BI/NAP1/027 C. difficile strain, its mechanism or pathogenesis, risk factors, current treatment options available, along with proposed preventative measures and infection control.
Co-Authors A. Soebandrio A. Widayati Adhiputra, I Ketut Agus Indra Adinda Putra Pradhana Agus Eka Darwinata Agus Roy Rusly Hariantana Hamid Agus Simahendra Agustinus I Wayan Harimawan Ajeng Diantini Amin, Yusuf Sidang Anggita Dewi Arikandini, Dewa Ayu Putu Aisaka Rajeshi Aryana, I Gusti Putu Suka Bryan Setiawan Candra, I Kadek Bayu Adhi Chandra, Katherine Silvania Christopher Ryalino D A Indah Gitaswari Dave Gerald Oenarta Dea Antariksa Dewa Ayu Agung Anggita Ningrat Dwija, Ida Bagus Nyoman Putra Dyah Pradnyaparmita Duarsa Eriata, Anselmus Helbert Erly Sintya Erly Sintya F. S. Wignall Fedik Abdul Ratam Gusti Ayu Dianti Violentina Hearty Indah Oktavian Hendrawan, Gresya I Dewa Agung Gede Meisha Dhanam I Dewa Ayu Made Dian Lestari I Dewa Made Sukrama I Gede Gita Sastrawan I Gede Pradnya Wisnu Murthi I Gusti Agung Indah Pradnyani R.S I Gusti Ayu Dewi Ratnayanti I Gusti Kamasan Arijana I Gusti Ngurah Kade Mahardika I Gusti Ngurah Mayun I Gusti Nyoman Sri Wiryawan I Made Ady Wirawan I Made Sutha Saskara I Nyoman Hariyasa Sanjaya I Nyoman Wande I Putu Bayu Mayura I Putu Bayu Mayura, I Putu Bayu I Wayan Adi Pranata I Wayan Rivandi Pradiyadnya Mardana I Wayan Sugiritama I Wayan Suranadi I. B. P. Dwija I. K. Sukardika I.K. Suata Ida Ayu Andhira Dewi Suarisavitra Ida Ayu Putu Putri Andari Ida Bagus Gede Adiguna Wibawa Ida Sri Iswari Indramawan Setyojatmiko Indraningrat, Anak Agung Gede K. Subrata K. Wirasandhi Kadek Anggie Wigundwipayana Kadek Dede Frisky Wiyanjana Kadek Karang Agustina Komang Januartha Putra Pinatih Krishna Murprayana Lameng, Imaculata Sonia Vidaryo M. A.C.M. Nusatya Made Agus Hendrayana Made Widianantara Made Wiryana Marco Reeiner N. K. Niti Susila, N. K. N. K. Susilarini Ni Kadek Seri Mahayanti Ni Luh Made Mirah Rahayu Ni Luh Putu Harta Wedari Ni Luh Putu Harta Wedari Ni Luh Putu Harta Wedari, Ni Luh Putu Harta Ni Luh Ranthi Kurniawathi Ni Made Adi Tarini Ni Made Linawati Ni Made Mertaniasih Ni Made Susilawathi Ni Nengah Dwi Fatmawati Ni Nengah Dwi Fatmawati Nyoman Indra Karunia Putri Nyoman Yenny K, Nyoman Pande Putu Januraga Prilandari, Luh Inta Prisela Zharaswati Prisillia Brigitta Putra, I Dw. Gd. Bayu Artha Pratama Putra, Made Dwi Ambara Putu Arya Suryanditha Putu Dian Pratita Lestari Putu Eka Arimbawa Raka-Sudewi A. A. Rastuti, Made Rina Rustawan, I Nengah Tony Saranova, Hilda Sari Kusumadewi Sathya Deva, I Dewa Gde Setiabudy, Marta Shita Diwyani Sudarsa Tjokorda Gde Agung Senapathi V. Wiwing Violentina, Gusti Ayu Dianti Wahyu Hidayati Wibisana, I Dewa Nyoman Adi Ningrat Widowati, I Gusti Ayu Rai - Wijaya, Carolin Winatha, I Gde Pangestu Putrama Winaya, Made Illene Wira Guna, I Gede Bhima Yanti, Ni Komang Semara Yuntari, Gusti Ayu Krisma