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All Journal Journal of Agromedicine and Medical Sciences Sosio Konsepsia Dentino: Jurnal Kedokteran Gigi Berkala Kedokteran Sari Pediatri Jurnal Sinergitas PkM & CSR MAGNA MEDICA: Berkala Ilmiah Kedokteran dan Kesehatan Bioinformatics and Biomedical Research Journal Prosiding Konferensi Nasional PKM-CSR Homeostasis: Jurnal Mahasiswa Pendidikan Dokter DUNIA KEPERAWATAN: JURNAL KEPERAWATAN DAN KESEHATAN
Lia Yulia Budiarti
Microbiology Department, Faculty Of Medicine Lambung Mangkurat University Banjarmasin
Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Control & Systems Engineering Dentistry Economics, Econometrics & Finance Education Engineering Environmental Science Health Professions Immunology & microbiology Medicine & Pharmacology Neuroscience Nursing Public Health Social Sciences

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Perbedaan Aktivitas Antibakteri Sabun Colek Merek Terpilih terhadap Jumlah Koloni Bakteri Staphylococcus aureus dan Escherichia coli Sri Widyarsi; Lia Yulia Budiarti; Farida Heriyani
Homeostasis Vol 4, No 3 (2021)
Publisher : Homeostasis

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 Abstract: Soap of colek is a cream soap that is often used by Sasirangan workers in Sungai Jingah Village, Banjarmasin to wash their hands after dyeing the Sasirangan cloth. The purpose of this study was to determine the differences in the activity of the 2 selected brands soap of colek on the number of bacterial colonies of Staphylococcus aureus and Escherichia coli. This study used a pure experimental method with a posttest-only design with a randomized group design. The treatments tested were soap dab selected brands A and B (concentrations of 0.5%, 1%, 12.5%, 25%, 50%, 75%, 100%) and sterile distilled water, with 3 times repetition of the treatment. The parameters observed were the number of colonies of Staphylococcus aureus and Escherichia coli on nutrient agar media. Data were analyzed using the Mann Whitney test (α.0.05). The results showed the difference in the average number of tested bacterial colonies after giving soap brand A and brand B soap of colek; all treatments soap of colek had significantly different activity in reducing the number of Staphylococcus aureus and Escherichia coli colonies; The treatment soap of colek A has a better activity in decreasing the number of tested bacterial colonies than soap of colek B. Conclusion, there are differences in the antibacterial activity of the 2 types of soap of colek selected test brands against the number of bacterial colonies of Staphylococcus aureus and Escherichia coli. Keywords: soap of colek, colony count, Staphylococcus aureus, Escherichia coli Abstrak: Sabun colek merupakan sabun krim yang sering digunakan para pekerja sasirangan di Kelurahan Sungai Jingah Banjarmasin untuk mencuci tangan setelah melakukan pencelupan kain sasirangan. Tujuan penelitian ini untuk mengetahui perbedaan aktivitas dari 2 merek sabun colek terpilih terhadap jumlah koloni bakteri Staphylococcus aureus dan Escherichia coli. Penelitian ini menggunakan metode eksperimental murni dengan rancangan posttest-only with randomized group design. Perlakuan yang diujikan adalah sabun colek terpilih merek A dan B (konsentrasi 0,5%, 1%, 12,5%, 25%, 50%, 75%, 100%) dan aquades steril, dengan pengulangan perlakuan sebanyak 3kali . Parameter yang diamati adalah jumlah koloni bakteri Staphylococcus aureus dan Escherichia coli pada media nutrient agar. Data dianalisis menggunakan uji Mann Whitney (α.0.05). Hasil penelitian didapatkan perbedaan rata rata jumlah koloni bakteri uji setelah pemberian sabun colek merek A dan merek B; semua perlakuan sabun colek memiliki aktivitas berbeda bermakna dalam menurunkan jumlah koloni Staphylococcus aureus dan Escherichia coli; perlakuan sabun colek A memiliki aktivitas lebih baik terhadap penurunan jumlah koloni bakteri uji daripada sabun colek B. Kesimpulan, terdapat perbedaan aktivitas antibakteri dari 2 jenis sabun colek merek uji terpilih terhadap jumlah koloni bakteri Staphylococcus aureus dan Escherichia coli. Kata-kata kunci: sabun colek, jumlah koloni, Staphylococcus aureus, Escherichia coli
Literature Review: Analisis Kualitas Air Sungai dengan Tinjauan Parameter pH, Suhu, BOD, COD, DO terhadap Coliform Amiratun Naillah; Lia Yulia Budiarti; Farida Heriyani
Homeostasis Vol 4, No 2 (2021)
Publisher : Homeostasis

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Abstract: The population growth in the river environment has an impact on the quality of river water so that the river has decreased in quality. This quality decline is influenced by the activities of the surrounding industries, hospitals, agriculture, settlements, trade. This study is done by analyzing related literature obtained from search results on medical journal databases, namely PubMed - MEDLINE, Cochrane library, and Google Scholar. Included articles are in English and published in 2010-2020. Total of 20 articles are included in this literature review. The data collected is the quality of river water based on pH, temperature, BOD, COD, DO parameters to total coliform. From the results of this literature review, it was found that pH and temperature did not have a significant relationship to the number of coliform bacteria in river water while BOD and COD had a significant relationship to the number of coliform bacteria. Furthermore, regarding DO parameters, there is no research that proves that DO has a relationship to the number of coliform bacteria in river water. Keywords: river, water quality, coliform. Abstrak:  Pertumbuhan populasi di lingkungan sungai memberikan dampak bagi kualitas air sungai sehingga sungai mengalami penurunan kualitas. Penurunan kualitas ini dipengaruhi oleh aktivitas perindustrian, rumah sakit, pertanian, pemukiman, perdagangan di sekitarnya. Penulisan ini dilakukan dengan menganalisis literatur terkait yang didapatkan dari hasil pencarian pada database jurnal kedokteran, yaitu PubMed – MEDLINE, Cochrane library, dan Google Scholar. Artikel yang disertakan menggunakan bahasa Inggris dan dipublikasikan pada tahun 2010-2020. Sebanyak 20 artikel disertakan pada literature review ini. Data yang dikumpulkan merupakan kualitas air sungai berdasarkan parameter pH, suhu, BOD, COD, DO terhadap total coliform. Dari hasil literature review ini didapatkan bahwa pH dan suhu tidak memiliki hubungan yang signifikan terhadap jumlah bakteri coliform dalam air sungai sedangkan BOD dan COD memilihi hubungan signifikan terhadap jumlah bakteri coliform. Selanjutnya, mengenai parameter DO belum ada penelitian yang membuktikan bahwa DO memiliki hubungan terhadap jumlah bakteri coliform pada air sungai. Kata-kata kunci: sungai, kualitas air, coliform.
Gambaran Jenis Bakteri Aerob pada Tinja Siswa Sekolah Dasar di Sekitar Bantaran Sungai Lulut Banjarmasin. Dastin Andre; Farida Heriyani; Lia Yulia Budiarti
Homeostasis Vol 2, No 1 (2019)
Publisher : Homeostasis

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Abstract: Bacteria in faeces contain normal flora and also pathogenic bacteria. The incidence of faecal-oral disease in South Kalimantan is high because of the faecal coliform in Martapura river are high. This study aims to describe type of aerobic bacteria in faeces of elementary students in Lulut riverbank which is Martapura river tributary. This research is a descriptive observational study. The data was taken from 30 faecal samples of students from Public Elementary School 3 and 7 Sungai Lulut Banjarmasin that have met the inclusion criteria. Results of this study concluded that there were 20 isolates of Escherichia coli (66.7%), Salmonella typhii 5 isolates (16.7%), and unculturable bacteria 5 isolates (16.7%) in faecal samples of students residing in riverbank, then Escherichia coli 18 isolates (60%), Salmonella typhii 4 isolates (13.3%), and unculturable ones 8 isolates (26.7%) in faecal samples of students residing outside riverbank. Keywords: Aerob bacteria, faeces, students, Lulut riverbank. Abstrak: Bakteri pada tinja anak dapat berupa flora normal maupun bakteri patogen. Kejadian faecal-oral disease di Kalimantan Selatan masih tinggi dikarenakan tingginya kandungan faecal coliform pada sungai Martapura. Penelitian ini bertujuan untuk mengetahui jenis bakteri aerob pada tinja siswa SD di sekitar bantaran Sungai Lulut Banjarmasin yang merupakan anak sungai Martapura. Penelitian bersifat observasional deskriptif. Subjek penelitian terdiri dari 30 sampel tinja siswa SDN 3 dan 7 Sungai Lulut Banjarmasin yang telah memenuhi kriteria inklusi. Hasil penelitian  terdapat Escherichia coli 20 isolat (66,7%), Salmonella typhii 5 isolat (16,7%), dan bakteri yang tidak tumbuh pada media 5 isolat (16,7%) pada sampel tinja siswa yang bertempat tinggal di bantaran Sungai Lulut, kemudian Escherichia coli 18 isolat (60%), Salmonella typhii 4 isolat (13,3%), dan bakteri yang tidak tumbuh pada media 8 isolat (26,7%) pada sampel tinja siswa yang bertempat tinggal di luar bantaran Sungai Lulut. Kata-kata kunci: Bakteri aerob, tinja, siswa, bantaran Sungai Lulut
Perbandingan Aktivitas Daya Hambat Sediaan Tunggal dengan Kombinasi Infus Phyllanthus niruri dan Peperomia pellucida terhadap Staphylococcus aureus Lie Vanny Leono; Edyson Edyson; Lia Yulia Budiarti
Homeostasis Vol 3, No 1 (2020)
Publisher : Homeostasis

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Abstract: Phyllanthus niruri and Peperomia pellucida traditionally used for treatment of a variety of diseases. Both of these plants are known to contain compounds that have antibacterial activity name flavonoids, alkaloids, tannins. Medicinal plant consumption can be used singly or in combination. The purpose of this study was to analyze the differences of  optimum inhibitory activity between a single and the combination of infusion of P. niruri and P. pellucida on the growth of S. aureus in vitro. This study method was a true experimental with post test-only design with control group design, consisting of 26 single and combination treatments of P. niruri concentrations at 5%, 15, 25%, 35% and P. pellucida concentration at 10%, 20%, 30 %, 40%. The controls used were vancomycin 30µg and distilled water. The parameter measured is the diameter of the inhibition zone. The data was analyzed used One-way ANOVA test and Duncan's post-hoc test with α = 0.05. The results showed there were significant differences from each treatment of single and combination infusion (p <0.05). The combination of P. niruri and P. pellucida infusion that produces optimum inhibitory zone effect greater than the single preparation found at Meniran 25% + P. pellucida 40%. The conclusion from this study is that the combination infusion have optimum inhibition greater than the single infusion. Keywords: single dose, combination dose, Phyllanthus niruri, Peperomia pellucida, Staphylococcus aureus, optimum inhibitory activity Abstrak: Meniran (Phyllanthus niruri) dan sirih cina (Peperomia pellucida) merupakan tanaman herbal yang digunakan masyarakat untuk mengobati berbagai macam penyakit.  Kedua tanaman ini diketahui mengandung senyawa yang bersifat antibakteri yaitu flavonoid, alkaloid, dan tanin. Sediaan tanaman obat dapat digunakan secara tunggal maupun kombinasi. Tujuan penelitian ini adalah menganalisis perbedaan aktivitas daya hambat optimum dari sediaan tunggal dan kombinasi infus meniran dan sirih cina terhadap pertumbuhan S. aureus secara in vitro. Metode penelitian ini adalah true experimental dengan rancangan post test-only with control group design, terdiri dari 26 perlakuan sediaan tunggal dan kombinasi infus pada konsentrasi meniran 5%, 15, 25%, 35% dan sirih cina 10%, 20%, 30%, 40%. Kontrol yang digunakan yaitu Vankomisin 30µg dan akuades. Parameter yang diukur yaitu diameter zona hambat. Analisis data menggunakan uji One-way ANOVA dan uji post-hoc Duncan dengan α = 0,05. Hasil penelitian didapatkan ada perbedaan bermakna dari masing-masing perlakuan sediaan tunggal dan kombinasi (p < 0,05). Sediaan kombinasi infus meniran dan sirih cina yang menghasilkan efek zona hambat optimum lebih besar daripada sediaan tunggalnya terdapat pada konsentrasi meniran 25% + sirih cina 40%. Simpulan dari penelitian ini adalah sediaan kombinasi memiliki daya hambat optimum lebih besar dibandingkan sediaan tunggalnya. Kata-kata kunci: sediaan tunggal, sediaan kombinasi, Phylanthus niruri, Peperomia pellucida, Staphylococcus aureus, Daya hambat optimum
Aktivitas Infus Kayu Apu (Pistia stratiotes) dalam Menurunkan Jumlah Bakteri Coliform pada Sampel Air Noor Rizka Yulia Rahmani; Siti Kaidah; Lia Yulia Budiarti
Homeostasis Vol 4, No 2 (2021)
Publisher : Homeostasis

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Abstract: Water lettuce (Pistia stratiotes) is an aquatic weed plant which contained antibiotic compounds and potentially to used as alternative disinfectant. This research was carried out to analyze the activity of water lettuce infusion in reducing the number of coliform bacteria colonies in the sample. The method in this research is a true experimental with pretest-posttest control group design approach. The concentrations of the infusion used are 50%, 75%, 100% (b/v). The data was analyzed with One-Way ANOVA and Post-hoc Duncan at the value of confidence of 95%. The research shows that there was a significant difference between MPN and TPC value index after the infusion added. The smallest value of MPN and TPC came from the 100% water lettuce infusion. Conclusion of this research, the water lettuce infusion has activity to decrease the number of coliform bacteria but its effectiveness is still below the 0.0002% of chlorine. Keywords: Pistia stratiotes, chlorine, MPN, TPC, coliform Abstrak: Kayu apu (Pistia stratiotes) merupakan tanaman gulma perairan yang mengandung senyawa antibakteri dan berpotensi untuk dimanfaatkan sebagai sediaan disinfektan alternatif. Tujuan penelitian ini menganalisis aktivitas infus kayu apu dalam menurunkan jumlah koloni bakteri coliform pada sampel air melalui uji MPN dan TPC. Metode penelitian eksperimental ini menggunakan rancangan pretest-posttest control group design. Perlakuan infus kayu apu 50%, 75%, 100% (b/v) diujikan terhadap sampel air perpipaan yang dimodifikasi dengan kandungan coliform setara Mac Farland 1. Data penelitian dianalisis menggunakan uji One-Way ANOVA dan uji Post-hoc Duncan pada tingkat kepercayaan 95%. Hasil penelitian, terbukti aktivitas antibakteri infus kayu apu mampu menurunkan jumlah coliform; ada perbedaan bermakna nilai indeks MPN dan TPC sampel air setelah pemberian perlakuan infus. Nilai indeks MPN dan TPC terkecil didapat dari perlakuan infus kayu apu 100%. Simpulan penelitian, infus kayu apu memiliki aktivitas dalam menurunkan jumlah bakteri coliform tetapi efektivitasnya masih di bawah klorin 0,0002%. Kata-kata kunci: Pistia stratiotes, klorin, MPN, TPC, Coliform.
Aktivitas Antibakteri Ekstrak Akar Binjai (Mangifera caesia Jack.) terhadap Shigella dysenteriae dan Salmonella typhi In Vitro Galuh Eka Suryani; Agung Biworo; Lia Yulia Budiarti
Homeostasis Vol 2, No 1 (2019)
Publisher : Homeostasis

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Abstract: Binjai is plant that’s widely spread in South Kalimantan. Binjai’s root contains saponins and tannins as antibacterial. The purpose of study to analyze the differences in inhibitory activity of binjai’s root ethanol extract against Shigella dysenteriae and Salmonella typhi in vitro. This study used true experimental studies that’s posttest with control group design, consisting of 10 treatments of binjai’s root ethanol extract (50%, 55%, 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95%) and ciprofloxacin (positive control). Data analysis was performed by one way ANOVA, post hoc Duncan, and t independent test (α = 0.05). The results of study showed that there were significant differences inhibitory power and inhibitory power increased with increasing concentration also at the same concentration, the inhibitory activity of binjai’s root ethanol extract was greater against to Salmonella typhi (23.12 mm) than Shigella dysenteriae (20.81 mm). Ethanol extract of binjai’s root has optimum inhibition at concentration 90% towards the growth both of bacteria. The conclusion is there are differences inhibitory activity of binjai’s root ethanol extract on the growth of Shigella dysenteriae and Salmonella typhi in vitro. Keywords: antibacterial activity, binjai’s root extract, Shigella dysenteriae, Salmonella typhi, in vitro. Abstrak Binjai merupakan tanaman yang banyak tersebar di Kalimantan Selatan. Akar binjai mengandung saponin dan tanin sebagai antibakteri. Tujuan penelitian ini adalah menganalisis perbedaan aktivitas daya hambat ekstrak etanol akar binjai terhadap Shigella dysenteriae dan Salmonella typhi secara in vitro. Penelitian ini menggunakan studi true experimental dengan rancangan posttest with control group design, terdiri dari 10 perlakuan ekstrak etanol akar binjai (50%, 55%, 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95%) dan siprofloksasin (kontrol positif). Analisis data dilakukan dengan uji one way ANOVA, post hoc Duncan, dan t independent  (α=0,05). Hasil penelitian menunjukkan terdapat perbedaan daya hambat yang bermakna dari masing-masing perlakuan dan daya hambat meningkat seiring dengan peningkatan konsentrasi serta didapatkan pada konsentrasi yang sama, aktivitas daya hambat ekstrak etanol akar binjai lebih besar terhadap Salmonella typhi (23,12 mm) dibandingkan Shigella dysenteriae (20,81 mm). Ekstrak etanol akar binjai memiliki daya hambat optimum pada konsentrasi 90% terhadap pertumbuhan kedua bakteri uji. Simpulannya adalah terdapat perbedaan aktivitas daya hambat ekstrak etanol akar binjai terhadap pertumbuhan Shigella dysenteriae dan Salmonella typhi secara in vitro. Kata-kata kunci: aktivitas antibakteri, ekstrak akar binjai, Shigella dysenteriae, Salmonella typhi, in vitro.
Perbandingan Potensi Antibakteri Infus Akar dari Tanaman Akar Kuning (Fibraurea tinctoria Lour.) terhadap Pseudomonas aeruginosa dan Escherichia coli Sonya Esti Kholifa; Lia Yulia Budiarti; Mohammad Bakhriansyah
Homeostasis Vol 4, No 1 (2021)
Publisher : Homeostasis

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Abstract: Fibraurea tinctoria Lour. plant is commonly used by Dayak tribes in Central Borneo as a herbal medicine. Previous studies showed that this plant contains alkaloids, flavonoids, saponins, terpenoids, tanins as antibacterial compounds. This study was aimed to analyze the comparison of antibacterial potency of Fibraurea tinctoria Lour. against Pseudomonas aeruginosa (P. aeruginosa) and Escherichia coli (E.coli). This research method was a true-experimental study with a post-test only with the control group design. Fibraurea tinctoria Lour. concentration were 15%, 35%, 50%, 75%, 100%. Ciprofloxacin 5 μg as a positive control and distilled water as a negative control. Parameters that measured in this study were the inhibitory zone diameter (milimeter) of bacterial growth. The data was analyzed using the One-Way ANOVA test and LSD Post-hoc test at the 95% confidence level. The results of this research showed that all concentration had antibacterial effect against P.aeruginosa and E.coli. In P.aeruginosa, the smallest inhibitory zone was 8.9 mm (35%) and E.coli 7.99 mm (15%); while the largest inhibitory zone (100%) was 18.62 mm in P. aeruginosa and 19.31 mm in E.coli. The conclusion is the antibacterial activity of Fibraurea tinctoria Lour. against E.coli was better than against P.aeruginosa. Keywords: antibacterial activity, yellow root infusion, Pseudomonas aeruginosa,  Escherichia coli. Abstrak:  Tanaman akar kuning (Fibraurea tinctoria Lour.) merupakan tanaman yang dimanfaatkan masyarakat Dayak di Kalimantan Tengah sebagai obat herbal alami. Penelitian terdahulu menunjukkan tanaman ini memiliki senyawa metabolit sekunder yang berperan sebagai antibakteri seperti flavonoid, alkaloid, saponin, terpenoid, dan juga tanin. Penelitian ini bertujuan untuk membandingkan potensi antibakteri infus akar kuning terhadap bakteri Pseudomonas aeruginosa (P.aeruginosa) dan bakteri Escherichia coli (E.coli). Metode yang digunakan true-experimental dengan post-test  with control group design. Perlakuan yang digunakan ialah sediaan infus konsentrasi (15%, 35%, 50%, 75%, 100%), siprofloksasin 5 μg, dan akuades masing-masing sebagai kontrol positif dan negatif. Parameter yang diukur pada penelitian ini ialah diameter zona hambat (milimeter) pertumbuhan bakteri. Data akan dianalisis menggunakan uji statistik One-Way ANOVA dan uji lanjutan Post-hoc LSD pada tingkat kepercayaan 95%. Hasil penelitian menunjukkan semua perlakuan memberikan efek antibakteri terhadap bakteri uji. Pada P.aeruginosa zona hambat terkecil 8,9 mm (35%) dan E.coli 7,99 mm (15%). Zona hambat terbesar (100%) yaitu 18,62 mm pada P.aeruginosa dan 19,31 mm pada E.coli. Kesimpulan penelitian ini adalah efek antibakteri infus akar kuning terhadap E.coli lebih baik dibandingkan terhadap P.aeruginosa. Kata-kata kunci: antibakteri, infus akar kuning, Pseudomonas aeruginosa, Escherichia coli.
Gambaran Jenis Bakteri pada Tangan Siswa Sekolah Dasar di Sekitar Bantaran Sungai Lulut Banjarmasin Pauline Surya Kurniati; Farida Heriyani; Lia Yulia Budiarti
Homeostasis Vol 2, No 1 (2019)
Publisher : Homeostasis

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Abstract: The presence of bacteria on the hands could cause the primary illness towards the body for example the infection of digestive system which proceeds to diarrhea, upper respiratory tract infection and skin diseases. Bacteria on the hands could differ from the location conducted. Therefore, this research is designed in order to obtain the hand’s bacteria of elementary students of the riverbank  Lulut Banjarmasin. The method that employed in this research was observational description alongside the cross sectional. The samples employed on this research were observed through 30 elementary students for each different groups, whom lived within less than 10 m from the riverbank and more than 10 m outside the riverbank. The result of this experiement through the swab hands towards the group of elementary students who lived near the riverbank showed that there were 70% of  Staphylococcus aureus and 30% of Escherichia coli. Whereas, the other group of elementary students who lived outside the area got 66.7% of Staphylococcus aureus and 33.3% of Escherichia coli. Through the analysis, it is indicated that Staphylococcus aureus was the most bacteria established on elementary hands students near the riverbank of Lulut River Banjarmasin. Keywords: Bacteria on hands, the riverbank, outside the riverbank, Lulut River Banjarmasin, elementary student Abstrak: Keberadaan bakteri pada tangan dapat menjadi perantara infeksi saluran cerna seperti diare, infeksi saluran pernapasan akut (ISPA) dan penyakit pada kulit. Jenis bakteri pada tangan dapat berbeda sesuai lokasi daerah. Penelitian ini bertujuan untuk mengetahui gambaran jenis bakteri pada tangan siswa Sekolah Dasar Negeri (SDN) di bantaran Sungai Lulut Banjarmasin. Metode penelitian ini adalah observasional  deskriftif dengan pendekatan cross sectional. Sampel yang digunakan dalam penelitian ini adalah masing-masing sejumlah 30 siswa siswa yang bertempat tinggal di bantaran dengan jarak ≤10 m dari sungai dan diluar bantaran dengan jarak ˃10 m dari sungai. Hasil penelitian pada swab tangan kelompok siswa sekolah dasar (SD) yang bertempat tinggal di bantaran sungai didapatkan Staphylococcus aureus sebanyak  70% dan Escherichia coli sebanyak 30%, sedangkan pada kelompok siswa SD yang bertempat tinggal di luar bantaran sungai didapatkan Staphylococcus aureus sebanyak  66,7% dan Escherichia coli sebanyak 33,3%. Dapat disimpulkan bahwa Staphylococcus aureus merupakan bakteri terbanyak pada tangan siswa SD disekitar bantaran Sungai Lulut Banjarmasin. Kata-kata kunci: Bakteri tangan, bantaran sungai, luar bantaran sungai, Sungai Lulut Banjarmasin, siswa sekolah dasar
Aktivitas Infus Eichornia crassipes Solms. (Eceng Gondok) terhadap Jumlah Koloni Staphylococcus aureus dan Escherichia coli Muhammad Bayu Fernanda; Siti Kaidah; Lia Yulia Budiarti
Homeostasis Vol 4, No 2 (2021)
Publisher : Homeostasis

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Abstract: The water hyacinth plant (Eichornia crassipes Solms.) is abundant aquatic plant that contain antibacterial compounds such as flavonoids and saponin. Antibacterial compounds can act as antiseptic. This study was aimed to analize infusion activities of water hyacinth in reducing the number of bacteriall colonies of Staphylococcus aureus and Escherichia coli. The study was a true experimental with pre and posttest with control group design,used  the water hyacinth infusion ekstract (20%, 40%, 60%, 80%, dan 100%) and alcohol 70%. Data was analyzed with one-way ANOVA and post hoc Duncan (α0.05). The result showed there was a decrease in  number of S.aureus and E.coli colonies after water hyacinth infusion; corresponds to increased concentration. The effect of water hyacinth infusion on S.aureus is larger than E.coli. The conclusion that water hyacibth infusion has activity to reduce the number of colonies of S.aureus and E.coli; effectiveness against S.aureus is greater than againt E.coli. Keywords: Eichornia crassipes Solms., number of bacteria colonies, Staphylococcus aureus, Escherichia coli, reculture isolates, hand swab.  Abstrak: Tanaman Eichornia crassipes Solms (eceng gondok) merupakan tanaman gulma air yang mengandung senyawa antibakteri flavonoid dan saponin, serta berpotensi dimanfaatkan sebagai antiseptik alternatif. Penelitian ini bertujuan menganalisis aktivitas infus eceng gondok dalam menurunkan jumlah koloni Staphylococcus aureus dan Escherichia coli, isolat bakteri uji hasil rekultur swab tangan. Rancangan penelitian ini menggunakan true experimental, pre and posttest with control group design, dengan perlakuan ekstrak infus eceng gondok 20%, 40%, 60%, 80%, dan 100%, serta alkohol 70 %. Analisis data menggunakan uji one-way ANOVA dan post-hoc Duncan (α.0.05). Hasil penelitian didapatkan penurunan jumlah koloni S.aureus dan E.coli sesudah pemberian infus eceng gondok; peningkatan konsentrasi berefek terhadap penurunan jumlah koloni bakteri uji. Efek infus eceng gondok terhadap S.aureus lebih besar dibandingkan terhadap E.coli. Simpulan, infus eceng gondok memiliki aktivitas menurunkan jumlah koloni S.aureus dan E.coli; efektivitas terhadap S.aurues lebih besar dibandingkan terhadap E.coli. Kata-kata kunci: Eichornia crassipes Solms, jumlah koloni bakteri, Staphylococcus aureus, Escherichia coli, isolat rekultur, swab tangan.
The Antibacterial Activity of Infusion of Averrhoa bilimbi L Fruits and Cananga odorata Flowers against Frequently Pathogenic Bacteria Lia Yulia Budiarti; Erida Wydiamala; Najiya Ulfa
Journal of Agromedicine and Medical Sciences Vol 8 No 3 (2022)
Publisher : Faculty of Medicine, University of Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/ams.v8i3.29312

Abstract

Infectious diseases due to opportunistic bacteria and pathogens are still a health problem. Transmission can be prevented by using an alcohol-based antiseptic liquid or naturally. Averrhoa bilimbi L. fruit plants and Cananga odorata flowers contain bioactive compounds that can inhibit bacterial growth. This study aimed to analyze the infusion activity of Averrhoa bilimbi fruit and Cananga odorata flower in single and combined dosage forms against several frequently pathogenic bacteria in vitro. This posttest-only experimental study with a control group design used the paper disc diffusion method. Observation parameters were the diameter of the inhibition zone on the test bacteria after infusion treatment of 50%, 75%, and 100% A.bilimbi fruit and 100% C.odora flowers, as well as 70% alcohol. The results showed that the diameter of the inhibition zone in the combination treatment of A.bilimbi and C.odorata infusion was significantly different from that of the single preparation (p<0.05). The combination of 100% A.bilimbi and 100% C.odorata (ratio 1:1) produced the greatest effect and was equivalent to 70% alcohol. The average inhibition of the combination infusion of Staphylococcus aureus (17.36mm) and Staphylococcus epidermidis (17.57mm) was greater than that of Escherichia coli (14.48 mm) and Salmonella enterica serovar Typhi (14.12mm). In conclusion, the infusion of Averrhoa bilimbi fruit and the Cananga odorata flower had antibacterial activity, and the combined preparation produced a better inhibitory effect than the single preparation. Keywords: antibacterial activity, Averrhoa bilimbi L., Cananga odorata, infusion, single preparation, combination preparation
Co-Authors A.A. Ketut Agung Cahyawan W Abdurahman Wahid Agung Biworo Agung Biworo Aisyah Aisyah Akbar Rihansyah, Akbar Alfi Yasmina Alfi Yasmina Alfia Fitriani, Alfia Alshazil, Renata Seikh Amalia, Maulidia Khairada Amalya, Khalida Zikra Amiratun Naillah Anward, Aliy Arivin Ari Yunanto Arietama, George Armanda, Ferdio Aulia Nalar, Gusti Ayu Apriliani Ayu Dewi Pertiwi Ayu Septiana Azhari, Nazla Puteri Chyntia Devi, Made Putri Dastin Andre Dayana, Puteri Dayani, Nor Ella Debby Saputera, Debby Derlin, Ellanda Permata Desy Elisa Kismiliansari, Desy Elisa Devin, Firdi Dewi Nurdiana Dhian Ririn Lestari Dhian Ririn Lestari, Dhian Ririn Diah Puspita Rifasanti Dita Permatasari Dwi Nur Rachmah Dwi Nur Rachmah Edi Hartoyo Edi Hartoyo Edyson Edyson Edyson Edyson, Edyson Eka Yudha Rahman Erida Wydiamala Erwin Rosadi, Erwin Fachriyad, Muhammad Fahdyannoor, Fahdyannoor Fahmi, Yafi Farida Heriyani Farida Heriyani Farida Heriyani Farida Raudah, Farida Galuh Eka Suryani Hafizhah, Ghina Hardiyanti Ruslan Hayatun Nufus Hikmah Ika Darmayanta Husna Dharma Putera, Husna Dharma Ichrom Nahzi, Muhammad Yanuar Ihsanti, Shofia Hilwa Ihya Ridlo Nizomy, Ihya Ridlo Ikhwanda Angga L., Ikhwanda Angga Isnaini Isnaini Isnaini Isnaini Joharman Joharman Khatimah, Husnul Khatimah3, Husnul Lena Rosida Lie Vanny Leono Lutfia Papita Derizky Rahmayanti M. Rizki Valian Akbar, M. Rizki Valian Maharani Laillyza Apriasari Mohammad Bakhriansyah Muhammad Bayu Fernanda Muhammad, Fadil Muthmainah, Noor Nadhila Nadhila Nadya Azzahra, Nadya Nadya Salsabila Nafiah Syella, Nafiah Nafilah Syella, Nafilah Najiya Ulfa Nasution, Naulita Sari Nisa, Rohmatun Noor Rizka Yulia Rahmani Norma Sari Normaida Novianti Nur Qamariah Nurlaili Rafina Nurwafa, Nurwafa Pauline Surya Kurniati Prenggono, Muhamad Darwin Puspa Astri Sella Putra, Andifa Anugerah Putri Putri, Putri Qiptiah, Putri Mariatul Rahmah, Resvi Amalia Ridhoni, Muhammad Zaki S., Nur Almira R. Safaana, Aurora Savitri, Dwiana Siddik, Muhammad Baihaqi Silvan Juwita Siti Kaidah Sonya Esti Kholifa Sri Hayati Nufaliana, Sri Hayati Sri Widyarsi Strata Pertiwi, Strata Sukma Noor Akbar Talitha Maghfira Ramadhinta, Talitha Maghfira Thea Shagita Ulfa, Najiya Wahyuni A, Wahyuni Widya Nursantari Wydiamala, Erida Wydiamala, Erida Zafira Aisyah Putri Zohra, Noor Fathimah