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All Journal International Journal of Power Electronics and Drive Systems (IJPEDS) International Journal of Renewable Energy Development POSITRON Jurnal Sains dan Pendidikan Fisika Jurnal Ilmu Ternak Veteriner WARTAZOA Indonesian Bulletin of Animal and Veterinary Sciences PROFIT : Jurnal Administrasi Bisnis Jurnal Fisika Unand JFA (Jurnal Fisika dan Aplikasinya) PROSIDING SEMINAR KIMIA Jurnal Fisika FLUX JURNAL BIOLOGI INDONESIA International Research Journal of Business Studies (E-Journal) Jurnal Penelitian dan Pengkajian Ilmu Pendidikan: e-Saintika Indonesian Journal of Electrical Engineering and Computer Science Edunesia : jurnal Ilmiah Pendidikan Computational and Experimental Research in Materials and Renewable Energy (CERiMRE) Makara Journal of Science Sewagati Jurnal Akuntansi Manado (JAIM) International Research Journal of Business Studies Journal of Engineering and Technological Sciences

Darminto .

Dept. Of Physics, Fac. Of Math. And Natural Sciences-Sepuluh November Institute Of Tech. Surabaya Kampus ITS Sukolilo, Surabaya 60111, Indonesia

Author-ID : 250221

Aerospace Engineering Arts Humanities Automotive Engineering Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Control & Systems Engineering Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Education Electrical & Electronics Engineering Energy Engineering Languange, Linguistic, Communication & Media Materials Science & Nanotechnology Mathematics Physics Public Health Social Sciences Veterinary Other

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Studies on the susceptibility of ostriches (Struthio camelus) to the Indonesian velogenic strain of Newcastle disease virus ., Darminto; Bahri, Sjamsul
Indonesian Journal of Animal and Veterinary Sciences Vol 2, No 4 (1998)
Publisher : Indonesian Animal Sciences Society

Susceptibility of ostriches (Struthio camelus) to the Indonesian velogenic strain of Newcastle disease virus (NDV) was evaluated by artificial infection . Twelve - 5 to 6 week old ostriches were divided into 3 groups each containing 4 birds . The first group was inoculated through respiratory system by dropping directly the virus solution into the nostrils, while the second group was inoculated through digestive system by dropping directly the virus solution into the oesophagus, with the dose of infection 106ELDSo (50%-embryo lethal dose) per bird . Meanwhile, the third group was treated as uninfected control . All infected birds developed antibody responses, but only two inoculated birds from the first group and two inoculated birds from the second group developed clinical signs of Newcastle disease (ND), with no specific pathological alterations . Infected birds, either sicks or healthy, excreted the challenge viruses through the respiratory system and still be detected up to the end of this experiment, ie . 15 days post-inoculation . The challenge viruses can be re-isolated from the brain, trachea, lungs, heart, liver, spleen, kidneys, small intestine, cecal-tonsil, and proventriculus of the infected birds . This study concludes that: (1) the ostriches are susceptible to the infection of the Indonesian velogenic strain ofNDV; (2) all infected birds developed immune responses, but only half of them develops el jtigi aj i disease ; (3) the infected birds excreted the challenge viruses for a considerable long time which may play role as the Mginiseti.ce ofinfectron the other healthy ostriches ; and (4) the challenge viruses can be re-isolated from various organs of the birds . . Â Keywords : Newcastle disease vir4, 9strich, immune response, artificial infection

Serotype variation among infectious bronchitis viral isolates taken from several areas of Java Indriani, Risa; ., Darminto
Indonesian Journal of Animal and Veterinary Sciences Vol 5, No 4 (2000)
Publisher : Indonesian Animal Sciences Society

Infectious bronchitis (IB) is an acute highly contagious viral respiratory disease of poultry caused by virus belongs to the family of Coronaviridae. The virus consist of many serotypes with low level of cross-protectivity among serotypes. Field data showed that the outbreaks of IB were frequently reported in chicken flocks, although vaccinations against the disease have been practiced. Hence, the study on serotype relationship among isolates of the viruses is essentially required. The aim of this study was to isolate and characterize IB viruses from chicken flocks in some areas of Java. Isolation of the virus was carried out in nine-day old embrionated chicken eggs and identified by means of agar gel precipitation (AGP) tests against standard antisera to IB virus. The serotypes of the IB viral isolates were determined by cross-neutralization tests in nine day old embryonated chicken eggs using r value derived from homologous and heterologous serum titres as criteria. This study obtained 12 IB viral isolates which were identified on the basis of the ability to cause lesions in chicken embryos and positive to agar gel presipitation test against standard positive antiserum to the virus. Based on the cross-neutralization tests in embryonated chicken eggs, isolate I.9 was formed to have relationship closed to Mass-41 serotype, while I.2, I. 3, and I.7 isolates were closely to the serotype of Con-46. Virus isolates (I.5, I.14, I.24, and I.25) were decided to have no serotype relationships to either Mass-41 or Con-46 serotype. Since the I.5, I.14, I.24 and I.25 isolates were not neutralized by antisera against the previous identified local infectious bronchitis viral isolates, and that were considered to be distinct serotype to the previously identified local IB viral isolates. Â Key words: Infectious bronchitis, virus, embryonated egg, cross neutralization test.

Antibody response and protection of inactivated-local isolate vaccine for infectious bronchitis in laying chicken Indriani, Risa; ., Darminto
Indonesian Journal of Animal and Veterinary Sciences Vol 6, No 2 (2001)
Publisher : Indonesian Animal Sciences Society

Infectious bronchitis (IB) is an acute highly contagious viral respiratory disease of poultry caused by Coronavirus. IBV infection consists of many serotypes and can only be controlled by vaccination. An effective IB vaccine should be prepared from local isolates, due to the antigenic variation among serotypes. The aims of this research were to develop inactivated IB vaccine derived from IBV local isolate and to determine the efficacy of that vaccine in layer flocks. Five layer chicken groups were used in this experiments, group I was vaccinated with commercial IBV live vaccine thrice, group II was vaccinated with commercial IBV live vaccine once and repeated with inactivated local IBV isolate twice, group III was vaccinated with commercial IBV live vaccine once and repeated with commercial inactivated twice, group IV was vaccinated with IBV live vaccine once, and group V was not vaccinated. After the chickens reached at a stable egg production they were challenged with IBV local isolates. Antibody responses were examined by means of haemagglutination hibitition (HI) test and HI titres were expressed as log2 of the reciprocal of the highest dilution of serum causing inhibition of a log2 HA titre of 2. The mean titres of antibody responses of chicken in group I, II, III, IV, and V was 4.9 Â± 0.87, 6.8 Â± 0.97, 7.7 Â± 0.46, 2.9 Â± 0.94, and 2.0 Â± 1.67 respectively. The levels of protection against challenges were determined by viral isolation, this in group I, II, III, IV, and V was 63, 73, 60, 50, and 0% respectively. Clinical symptom of egg quality was slightly reduced in group I, IV, and V and it were unchanged in group II and III. Group II gave better in number of egg Â production than the other groups. The results indicated that the IBV inactivated localisolate vaccine gave high titres of Â antibody and higher protection rates than that of commercial IBV inactivated vaccine. Inaddition, IBV local isolate vaccinated group prevented from declining egg production after challenged with IBV local isolate. Â Key words: Infectious bronchitis, layer, antibody titre, vaccine, challenge virus

The detection of infectious bronchitis viral antigen by means of immunohistochemical technique in broiler chicken infected with I-269 IB isolate or injected with H-120 live vaccine Damayanti, Rini; ., Darminto
Indonesian Journal of Animal and Veterinary Sciences Vol 6, No 4 (2001)
Publisher : Indonesian Animal Sciences Society

A study was carried out to detect the antigen of infectious bronchitis vius (IBV) in broiler chicken by means of immunohistochemical technique. A total of 150 - fourteen days old broiler chicken were divided into three groups i.e. 50 chicken were infected with an IB isolate of I-269, 50 chicken were injected with H-120 life vaccine, and 50 chicken served as un-treated control. Clinical signs and gross pathological changes were observed. Each of five chicken of each group were necropsied at 1, 2, 3, 4, 7, 10, 14, 21, 28, and 35 day(s) post infection/vaccination. The antigen could be detected at one day through 35 days post vaccination/infection. In the vaccinated group, histopathological lesions and the detected antigen were minimal. In contrast, the infected chicken showed varied histolopathological lesion in accordance with the numerous antigens. The antigen were observed in the lymphocytes/macrophages in the trachea, lungs and kidney, and in the epithelium of trachea, alveoli, broncheolus and tubular sitoplasm of the kidney of both vaccinated and infected groups. In the infected group, antigen was also detected in the lymphocytes and macrophages of the affected organs. Â Key words: Infectious bronchitis, broiler chicken, I-269 IB isolate, immunohistochemistry

The development of an Enzyme Linked Immunosorbent Assay for detecting Injectious laryngotrachitis viral antibodies in chicken serum Indriani, Risa; Adjid, R.M Abdul; ., Darminto; Hamid, Helmy
Indonesian Journal of Animal and Veterinary Sciences Vol 7, No 2 (2002)
Publisher : Indonesian Animal Sciences Society

The aim of this study was to develop an Enzyme-linked immunosorbent assay (ELISA) for detection of antibody against gallid herpes virus, the causal agent of infectious laryngotracheitis (ILT) in chicken. Its application in experimental chicken under laboratory condition was also evaluated. Results showed that ELISA for ILT was developed successfully with sensitivity and specificity was 98% and 97,14% respectively. The ELISA was able to determine the dynamic of antibodies respond in experimental chickens following vaccination and artificial infection with ILT virus. It was concluded that this ELISA offers a simple, sensitive and specific antibody assay for detection of antibodies against ILT virus in chickens arising from vaccination or infection. Â Key words: ELISA, antibody, chicken, Infectious laryngotrachitis

Purification of neuraminidase from Influenza virus subtype H5N1 Tarigan, Simson; Indryani, Risa; ., Darminto; Ignjatovic, Jagoda
Indonesian Journal of Animal and Veterinary Sciences Vol 14, No 1 (2009)
Publisher : Indonesian Animal Sciences Society

Influenza-virus neuraminidase plays vital role in the survival of the organisms. Vaccination of animals with this glycoprotein confers immune responses so that enable it to protect the animals from incoming infection. Supplementation of conventional vaccines with this glycoprotein increases the protection and longevity of the vaccine. Purified neuraminidase can also be used to develop serological tests for differentiation of serologically positive animals due to infection or to vaccination. In this study purification of neuraminidase from influenza virus subtype H5N1 was described. Triton x-100 and Octyl Î²-D-glucopyranoside were used to extract and diluted the glycoprotein membrane. The enzymatic activity of the neuraminidase was assayed using a fluorochrome substrate, 4-methylumbelliferyl-a-D-N-acetyl neuraminic acid, which was found to be simple, sensitive and suitable for the purification purpose. The neuraminidase was absorbed selectively on an oxamic-acid agarose column. The purity of neuraminidase eluted from this affinity column was high. A higher purity of the neuraminidase was obtained by further separation with gel filtration on Superdex-200. The purified neuraminidase was enzymatically active and did not contain any detectable haemagglutinin, either by haemagglutination assay or by monospecific antibodies raised against H5N1 hemagglutinin.Â The purified neuraminidase was recognized strongly by antibodies raised against an internal but only weakly by that against C-terminal regions of the neuraminidase protein of H5N1-influenza virus. The purified neuraminidase was in tetrameric forms but dissociated into monomeric form on reducing condition, or mostly dimeric form on non-reducing SDS-PAGE. Key Words: Neuraminidase, Influenza, H5N1, Methylumbelliferyl, Oxamic-acid

Epidemiology of JapaneseâBâ encephalitis infection in pigs in Riau and North Sumatera Provinces Sendow, Indrawati; Syafriati, Tatty; Hadi, Upik Kesumawati; Malole, Martin; Soviana, Susi; ., Darminto
Indonesian Journal of Animal and Veterinary Sciences Vol 8, No 1 (2003)
Publisher : Indonesian Animal Sciences Society

Epidemiology study on Japanese-B-Encephalitis (JE) was conducted in Riau and North Sumatera Provinces. A total of 190 pig sera from Riau Province and 164 pig sera from North Sumatera were tested using competitive ELISA (C-ELISA) to detect antibodies against JE virus. Insect collection was also conducted using several methods near pig farms in those provinces and identified into species to gain more information on its role to distribute JE infection. Serological results indicated that 70% pig in Sumatera and 94% pig in Riau had antibodies against JE virus. The highest prevalence of reaktor was detected in pig of more than 4 months age in both Provinces. The results of insect collection showed that Culex tritaeniorchynchus and Culex quinquefasciatus were the most dominant species in both provinces. Based on serological testing, indicated that JE virus infected pig in Sumatera and Riau Provinces, and higher reactor was obtained in older pig. Culex tritaeniorchynchus and Culex quinquefasciatus were the dominant insect species in both provinces, hence those species had a possibility to play an important role of JE transmission. Â Key words: JE, pigs, serology, insects

Comparison of sequences of hypervariable region (HVR) subunit S-1 gene of field isolate I-37 infectious bronchitis virus with Connecticut serotype Dharmayanti, N.L.P Indi; Indriani, Risa; ., Darminto
Indonesian Journal of Animal and Veterinary Sciences Vol 8, No 2 (2003)
Publisher : Indonesian Animal Sciences Society

Infectious Bronchitis is a contagious and acute respiratory disease in chickens caused by infectious bronchitis virus (IBV).Antigenic differences in IBV are associated with changes in the sequence of the spike glycoprotein (S). The subunit S1 which demonstrates more sequence variability than S-2 have been identified as hypervariable region (HVR-1 and 2). There were several IB virus field isolates included I-37 have been identified in Indonesia by serum neutralization method. However, gene sequence variation in HVR subunit S-1 had not yet been identified. Isolate I-37 was close to the serotype Connecticut 46 (Conn 46). The aim of this study is to identify sequence variation of HVR subunit S-1 gene of isolate I-37 produced by Reverse Transcriptase-Polymerase Chain Reaction (RT-PCR) and sequencing. Several procedures were carried out in the study including virus titration, propagation and was concentrated from the allantoic fluid infected with IBV. Then, RNA was extracted for RTPCR. urther the product was sequnced and its homology with IBV references from GenBank was compared by GenMac version 8.0. Result showed that isolate I-37 produced 515 bp of amplification product. Isolate I-37 and Conn 46 are same serotype, yet their HVR subunit S-1 nucleotides and amino acids (protein) differ by 6.9% and 15.6% respectively. It might be concluded that isolate I-37 was variant of Conn 46. Â Key words: Sequences variation, IBV, I-37 field isolate, HVR subunit S-1 gene

Detection of avian influenza virus H5N1 subtype in organs of chicken affected by higly pathogenic avian infuenza in East and West Java by using immunohistochemical technique Damayanti, R; Dharmayanti, N.L.P.I; Indriani, R; Wiyono, A; ., Darminto
Indonesian Journal of Animal and Veterinary Sciences Vol 9, No 3 (2004)
Publisher : Indonesian Animal Sciences Society

The study was conducted to detect antigen H5N1 of highly pathogenic Avian Influenza (HPAI) virus in various farms in East and West Java. The immunohistochemical technique was applied due to Hematoxilin-eosin (H&E) staining was impossible to visualize the antigen in tissue. Immunohistochemical staining was applied for some visceral organs collected from the areas where the outbreaks occurred in September-October 2003. The specimens were processed as histopathological paraffin blocks using standard method. The blocks that were suspected to have antigen H5N1 were cut and rabbit antisera to H5N1 produced from the local isolate was applied as the primary antibody. Biotinylated secondary antibody and avidin biotin peroxidase from a commercial kit were administered. The antigen present in the tissues were visualized by adding a substrate called Amino Ethyl Carbazole (AEC) resulting in reddish brown colour. This immunostaining proved to be accurate and reliably quick method to detect H5N1 antigen present in the avian tissues. In conclusion, the outbreak of bird flu was caused by H5N1 strain and the antigen could be found in wattles, combs, brain, trachea, lungs, heart, proventriculus, liver, spleen, kidney and ovary. Â Key words: Highly pathogenic Avian Influenza (HPAI), chicken, H5N1, outbreak, immunohistochemistry

Co-Authors A Ratnawati A Wiyono abdurrouf Abdurrouf Adler Haymans Manurung Agung Suwandaru Agus Wiyono Ahzan, Sukainil Alimuddin Ali Anne Zulfia Arifin, Rizal Bambang Subali Budhi Priyanto Budi Priyanto Cahyoingrat, Suryo E. Cahyono, Yoyok D.A Hewajuli Dadan Hamdani, Dadan Daniels, Peter Daniels, Peter Dedi Supriadi Dharmayanti, N.L P.I. Dharmayanti, N.L P.I. Dharmayanti, NLP Dharmayanti, NLP Eddy S Siradj Eko Handoyo Endang Siti Astuti Endhah Purwandari Fahmi Astuti, Fahmi Fatimah Karunia Sultera Field, Hume Field, Hume Firdaus, Aulia Anisa Gatut Yudoyono H Hamid Haniffudin Nurdiansah Hartawan, R. Hartawan, R. Helmy Hamid Hesti Irnanta Hewajuli, D.A. Hewajuli, D.A. I Wayan T. Wibawan Ida Ayu Putu Sri Widnyani Ida Widaningrum, Ida Indrawati Sendow Intifadhah, Sahara Hamas Iwan Dini Iwan Yahya Jagoda Ignjatovic Johari, Norhasnidawani Joni Dwi Pribadi Kholis Nurhanafi Kolif Yulianto L Parede Lee, Vannajan Sanghiran Lintang Edityastono Malik Anjelh Baqiya Manik, Tetti Novalina Martin Malole Mashuri Mashuri Mochammad Zainuri Morrissy, Chris Morrissy, Chris Muhammad Taqiyuddin Muhammad Zainuri Muharam Saepulloh Munir, Rahmawati Muzakki, Fikrun Najib N. Suryana N.L.P Indi Dharmayanti N.L.P.I Dhamaryanti N.L.P.I Dharmayanti N.L.P.I. Dharmayanti Ninis Hadi Haryanti, Ninis Hadi NLP Indi Dharmayanti Nugroho, Ferry Anggoro Ardy Nuni Widiarti Nurma Sari, Nurma Nurshabrina, Atiqa Taqiyya P Ronohardjo Prayogi, Saiful Prayogi, Soni Puspita, Niniek Fajar R Damayanti R Indriani R M A Adjid R. Indriani R.M Abdul Adjid R.M. Abdul Adjid R.M.A. Adjid Ratnawati, A. Ratnawati, A. Retno Asih, Retno RISA INDRIANI Risa Indryani Risdiana, Risdiana Rizoputra, Ivan Rizqy Wahyu Septiono S Hardjoutomo Saleh, Muh Santoso, Tanto Budi Shaliha, Shaliha Shinta Maharani Trivena Sholih, Ahmad Simon Sadok Siregar, Simon Sadok Simson Tarigan Siti Ragil Handayani Sitorus, Mido Ester Sjamsul Bahri SRI WARDANI Suarso, Eka Suhadak Suhadak Suminar Pratapa Suryajaya Suryajaya, Suryajaya Susan M Noor Susi Soviana T Sjafriati T Syafriati Taher Al habsji Tatty Syafriati Totok Wianto Upik Kesumawati Hadi W. Asmara W. T. Artama, W. T. Wahyudi, Sriati Winardi, Yoyok Zainul Arifin Zainuri, M Zulkarnain, Zulkarnain

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