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All Journal HAYATI Journal of Biosciences Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Buletin Agrohorti Jurnal Ilmu Pertanian Indonesia Jurnal Hortikultura Indonesia (JHI) Jurnal Silvikultur Tropika (JST) Agrotrop : Journal on Agriculture Science Bionatura Zuriat Jurnal Natur Indonesia Atom Indonesia Journal Jurnal Ilmiah Aplikasi Isotop Radiasi Jurnal Agro Teknologi Tropika Jurnal Ilmu Komputer dan Agri-Informatika Jurnal Hortikultura Jurnal AgroBiogen Jurnal Penelitian Tanaman Industri Buletin Penelitian Tanaman Rempah dan Obat Pelita Perkebunan Jurnal Agroekoteknologi Jurnal Agro Jurnal Penelitian Karet Tropical Animal Science Journal Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Journal of Tropical Crop Science JURNAL BUDIDAYA PERTANIAN Jurnal Agrotek Lestari Jurnal Penelitian Tanaman Industri (Littri) Buletin Penelitian Tanaman Rempah dan Obat Warta Perkaretan Jurnal Sains Terapan : Wahana Informasi dan Alih Teknologi Pertanian Jurnal Natural
Desta Wirnas
Departemen Agronomi Dan Hortikultura, Fakultas Pertanian, Institut Pertanian Bogor (IPB University), Jl. Meranti, Kampus IPB Darmaga, Bogor 16680, Indonesia
Agriculture, Biological Sciences & Forestry Astronomy Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Education Energy Environmental Science Immunology & microbiology Industrial & Manufacturing Engineering Materials Science & Nanotechnology Neuroscience Physics Social Sciences Veterinary

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Evaluating Genetic Variability of Sorghum Mutant Lines Tolerant to Acid Soil W. Puspitasari; S. Human; D. Wirnas; Trikoesoemaningtyas Trikoesoemaningtyas
Atom Indonesia Vol 38, No 2 (2012): August 2012
Publisher : PPIKSN-BATAN

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17146/aij.2012.165

Abstract

High rainfall in some parts in Indonesia causes soil become acidic. The main constraint of acid soil is phosphor (P) deficiency and aluminum (Al) toxicity which decrease plant productivity. To overcome this problem, it is important to develop a crop variety tolerant to such conditions. Sorghum is probably one of the potential crops to meet that objective. Sorghum has been reported to have wide adaptability to various agro-ecology and can be used as food and animal feed. Unfortunately, sorghum is not Indonesian origin so its genetic variability is still low. From previous breeding works with induced mutation, some promising mutant lines have been developed. These mutant lines were included in the experiment carried out in Tenjo with soil condition was classified as acid soil with pH 4.8 and exchangeable-Al content 2.43 me/100 g. The objectives of this experiment were to study the magnitude of genetic variability of agronomy and grain quality characters in sorghum in order to facilitate the breeding improvement of the species. Plant materials used in this study were ten genotypes, including 6 mutant lines and 4 control varieties. The randomized block design with three replications was used in the experiment. The genetic variabilities of agronomic and grain quality characters existed among genotypes, such as plant height, number of leaves, stalk diameter, biomass weight, panicle length, grain yield per plant, 100 seed weight and tannin content in the grain. The broad sense heritabilities of agronomic characters were estimated ranging from medium to high. Grain yield showed significantly positive correlation with agronomic characters observed, but it was negatively correlated with protein content. Received: 05 December 2011; Revised: 21 June 2012; Accepted: 02 July 2012
Keragaan Fenotipik Kedelai pada Dua Kondisi Intensitas Cahaya Ekstrim WAGE RATNA ROHAENI;   TRIKOESOEMANINGTYAS; DESTA WIRNAS
Agrotrop : Journal on Agriculture Science Vol 4 No 1 (2014)
Publisher : Fakultas Pertanian Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (23.435 KB)

Abstract

Phenotypic of Soybean at Two Extreme Condition of Light Intensity.Cultivation of soybean inrow rubber area was one of extensification programme for improving national soybean production. Theproblem on row rubber area was the low light intensity that could disturb soybean growth. Plant breedingfor creating tolerant variety for low light intensity was doing until now. Bogor Agricultural University hadmany lines for being candidate of tolerant variety. F6 soybean population from single seed descentmethode need to explored for that trait on low light intensity condition. The aim of this research was toknow the differences of soybean phenotypic between on low light intensity and full light intensity condition.The research was done on March – May 2009. Randomized complete block design with 3 replicationwas using as experimental design which lines as the treatment on two condition (low light and full lightcondition). The result saw that low light intensity was changing type of soybean growth, determinatebecame indeterminate type of growth. Low light intensity made soybean became early on floweringfase and water content on seed was increase. Low light intensity made decreasing the productivity until37.25% than full light intensity condition.
The Relationship of Nutmeg Populations from Tidore, Ternate, and Bogor Based on Morphological Marker Tias Arlianti; Desta Wirnas; NFN Sobir; Otih Rostiana
Buletin Penelitian Tanaman Rempah dan Obat Vol 30, No 2 (2019): Buletin Penelitian Tanaman Rempah dan Obat
Publisher : Pusat Penelitian dan Pengembangan Perkebunan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/bullittro.v30n2.2019.69-80

Abstract

Banda Nutmeg (Myristica fragrans), is one of Indonesia's main spices commodities. Maluku Island, North Maluku, Siau, and Papua are the center of origins and center of nutmeg diversity; whereas, Bogor is the largest nutmeg cultivation area in West Java. The diversity and relationship between Bogor nutmeg with Maluku nutmeg have not been studied, even though it is crucial for local varieties selection and seeds provision. The study aimed to determine the diversity and relationship of nutmegs from Tidore, Ternate, and Bogor. The experiment was conducted in eight locations: Tidore (Gurabunga and Jaya), Ternate (Marikurubu), and Bogor (Cigombong, Ciawi, Leuwisadeng, Sukajadi, and Tamansari) from November 2017 - December 2018. Materials used were 46 nutmeg accessions of 8 – 30 year old plants with good growth and known of their origin. The experiments were performed using direct observation methods on habitus, leaf, fruit, seed, mace,  and flower followed IPGRI descriptor.  The results showed that qualitative diversity was observed in the fruit shape, shape of fruit-based and fruit-tip, fruit color, and tree shape. Mace thickness was the most substantial diversity for the quantitative character (50.38 %). The difference within intra-population in all aspects observed was low, except for the fruit character and mace weight. The genetic relatedness of the Bogor population was closer to Ternate (60 %) than Tidore (46 %). The genetic relationship amongst five Bogor populations found to be very close. Further, Leuwisadeng, Tamansari, and Sukajadi populations were found to have the highest genetic relationship and similarity (80 %).
ANALISIS DAYA HASIL LATEKS DAN HERITABILITAS KARAKTER KUANTITATIF DARI BEBERAPA GENOTIPE KARET PP/07/04 Sayurandi Sayurandi; Desta Wirnas; Sekar Woelan
Jurnal Penelitian Karet JPK : Volume 34, Nomor 1, Tahun 2016
Publisher : Pusat Penelitian Karet - PT. Riset Perkebunan Nusantara

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22302/ppk.jpk.v34i1.226

Abstract

Tujuan penelitian ini adalah untuk mempelajari karakter pertumbuhan, daya hasil lateks dan heritabilitas karakter kuantitatif  beberapa genotipe karet  PP/07/04. Sebanyak lima belas  genotipe karet dan dua klon pembanding PB 260 dan RRIC 100 diuji pada penelitian ini. Pengujian genotipe tersebut dibangun di Kebun Percobaan Balai Penelitian Sungei Putih, Pusat Penelitian Karet pada tahun 2004, yang terletak di Kabupaten Deli Serdang - Provinsi Sumatera Utara. Rancangan yang digunakan pada penelitian ini adalah Rancangan Acak Lengkap (RAL) dengan tiga ulangan. Hasil penelitian menunjukkan bahwa genotipe berpengaruh nyata terhadap dua belas karakter yang diamati yaitu lilit batang, tebal kulit, jumlah cincin pembuluh lateks, diameter sel pembuluh lateks, panjang alur sadap, kecepatan aliran lateks, indeks penyumbatan, kadar fosfat anorganik, kadar sukrosa, kadar thiol, kadar karet kering, dan  daya hasil lateks. Berdasarkan karakter pertumbuhan dan daya hasil lateks menunjukkan bahwa genotipe HP 92/309 memiliki potensi hasil lateks tinggi, sedangkan genotipe HP 92/542 memiliki hasil lateks tinggi dan pertumbuhan jagur. Dua belas karakter yang diamati memiliki nilai heritabilitas tinggi dengan nilai h2bs antara 0,52 – 0,95. Hal ini menunjukkan bahwa karakter-karakter tersebut lebih dipengaruhi oleh faktor genetik. Diterima : 5 April 2016 / Direvisi : 30 Juni 2016 / Disetujui : 3 Agustus 2016 How to Cite : Sayurandi, S., Wirnas, D., & Woelan, S. (2016). Analisis daya hasil lateks dan heritabilitas karakter kuantitatif dari beberapa genotipe karet PP/07/04. Jurnal Penelitian Karet, 34(1), 1-12. Retrieved from http://ejournal.puslitkaret.co.id/index.php/jpk/article/view/226
Pewarisan Karakter Daya Simpan Benih Kedelai pada Kondisi Suhu Ruang (25°C) Desta Wirnas; Eni Widajati; Atika Bakti Sari
Jurnal Agroekoteknologi Vol 4, No 1 (2012)
Publisher : Jurusan Agroekoteknologi Fakultas Pertanian Untirta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (364.665 KB) | DOI: 10.33512/j.agrtek.v4i1.613

Abstract

ABSTRACTThe objective of the research was to obtain information on inheritance of characters related seed soybean storability. Genetic material used were 10 varieties and 10 breeding lines. The research was arranged in randomized completely block design with three replicates. The results showed that all characters observed was significantly affected by genotypes. Electrical conductivity showed the highest heritability among the characters observed. Some genotypes (B.3749, B.3641, Tanggamus, and Burangrang) showed good storability after 2 month stored period under room temperature conditions.Key words: soybean, seed storability, heritability
ISOLASI FRAGMEN GEN PENYANDI PUTRESIN N-METILTRANSFERASE DAN QUINOLINAT FOSFORIBOSILTRANSFERASE ASAL TEMBAKAU LOKAL TEMANGGUNG (Nicotiana tabacum) SESANTI BASUKI; NURHAJATI AA MATTJIK; SUWARSO SUWARSO; DESTA WIRNAS; SUDARSONO SUDARSONO
Jurnal Penelitian Tanaman Industri Vol 17, No 3 (2011): September 2011
Publisher : Pusat Penelitian dan Pengembangan Perkebunan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jlittri.v17n3.2011.109-117

Abstract

ABSTRAKUpaya untuk menurunkan kandungan nikotin merupakan salah satuprioritas utama penelitian tembakau. Nikotin adalah senyawa alkaloidutama berpotensi dikonversi menjadi senyawa nor-nikotin yang bersifatkarsinogen. Gen PMT sebagai penyandi enzim putresin n-metiltransferase(PMT) dan gen QPT - penyandi enzim quinolinat fosforibosiltransferase(QPT) merupakan dua gen kunci yang berperan penting pada proses bio-sintesis nikotin. Penelitian ini bertujuan untuk mengisolasi potongan genPMT dan QPT asal tembakau lokal Indonesia, mengkarakterisasi danmenganalisis runutan DNA-nya. Tahapan penelitian dimulai dengan me-rancang primer degenerate berdasarkan informasi yang ada di pangkalandata Bank Gen NCBI (National Centre for Biotechnology Information),mengamplifikasi PCR menggunakan templat DNA genomik tembakaulokal cv. Sindoro1, mengklon potongan DNA hasil PCR dan menentukanrunutan DNA-nya. Hasil penelitian menunjukkan dari dua belas pasangprimer degenerate yang dirancang, hanya dua pasang primer yang meng-hasilkan potongan DNA hasil amplifikasi PCR, yaitu pasangan primerPMt-7 (F & R) untuk gen PMT dan primer QPt-3 (F & R) untuk gen QPT.Setelah dilakukan penentuan runutan DNA-nya, amplikon yang didapatdari hasil PCR dengan pasangan primer PMt-7 sebesar 1418 bp, sedangkanuntuk primer QPt-3 sebesar 205 bp. Runutan DNA gen PMT dan gen QPTasal tembakau lokal cv. Sindoro1 mempunyai tingkat kesamaan yang ting-gi dengan gen PMT dan gen QPT asal tembakau lainnya yang ada dipangkalan data Bank Gen NCBI.Kata kunci : Gen PMT, gen QPT, lintasan biosintesis nikotin, perunutanDNA, amplifikasi PCR, primer degenerateABSTRACTIsolation of Genes encoding Putrescine N-Methyl-transferase and Quinolinat Phosphoribosyl transferasederived from Temanggung Tobacco Cultivar (Nicotianatabacum)Reduction of nicotine content is one of the major objective intobacco research. Nicotine is the main alcaloid compound that potentiallycould be converted into a carcinogenic compound (nor-nicotine). The PMTgene encoding putrescine N-methyl transferase (PMT) and the QPT gene -encoding quinolinate phosphoribosyl transferase (QPT) are the two keyenzymes involved in nicotine biosynthesis. The objectives of this researchwere to isolate PMT and QPT gene fragments originated from Indonesianlocal tobacco, to characterize, and to analyze their DNA sequences. Theresearch activities included: degenerate primer design based oninformation available in the GenBank DNA Database NCBI (NationalCentre for Biotechnology Information), PCR amplification usingdegenerate primer and genomic DNA template of a local tobacco cv.Sindoro1, clone the PCR amplified products, and determine their DNAnucleotide sequences. Results of the experiment indicated that from 12degenerate primer pairs synthesized, only two were able to yield positivePCR amplified products. These primer pairs were PMt-7 (F & R primers)for PMT and QPt-3 (F & R primers) for QPT. After DNA sequencing, theamplified DNA product amplified using PMt-7 degenerate primer pairswere 1418 bp, while that using QPt-3 primer pairs were only 205 bp.Nucleotide sequences of PMT or QPT gene fragments originated fromlocal tobacco cv. Sindoro1 showed a high nucleotide sequences identity ascompared to that of the respective genes from other tobacco species thatwere available in the GenBank DNA Database NCBI.Key words: PMT gene, QPT gene, nicotine biosynthetic pathways, DNAsequencing, PCR amplification, degenerate primer
The Relationship of Nutmeg Populations from Tidore, Ternate, and Bogor Based on Morphological Marker Tias Arlianti; Desta Wirnas; NFN Sobir; Otih Rostiana
Buletin Penelitian Tanaman Rempah dan Obat Vol 30, No 2 (2019): Buletin Penelitian Tanaman Rempah dan Obat
Publisher : Pusat Penelitian dan Pengembangan Perkebunan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/bullittro.v30n2.2019.69-80

Abstract

Banda Nutmeg (Myristica fragrans), is one of Indonesia's main spices commodities. Maluku Island, North Maluku, Siau, and Papua are the center of origins and center of nutmeg diversity; whereas, Bogor is the largest nutmeg cultivation area in West Java. The diversity and relationship between Bogor nutmeg with Maluku nutmeg have not been studied, even though it is crucial for local varieties selection and seeds provision. The study aimed to determine the diversity and relationship of nutmegs from Tidore, Ternate, and Bogor. The experiment was conducted in eight locations: Tidore (Gurabunga and Jaya), Ternate (Marikurubu), and Bogor (Cigombong, Ciawi, Leuwisadeng, Sukajadi, and Tamansari) from November 2017 - December 2018. Materials used were 46 nutmeg accessions of 8 – 30 year old plants with good growth and known of their origin. The experiments were performed using direct observation methods on habitus, leaf, fruit, seed, mace,  and flower followed IPGRI descriptor.  The results showed that qualitative diversity was observed in the fruit shape, shape of fruit-based and fruit-tip, fruit color, and tree shape. Mace thickness was the most substantial diversity for the quantitative character (50.38 %). The difference within intra-population in all aspects observed was low, except for the fruit character and mace weight. The genetic relatedness of the Bogor population was closer to Ternate (60 %) than Tidore (46 %). The genetic relationship amongst five Bogor populations found to be very close. Further, Leuwisadeng, Tamansari, and Sukajadi populations were found to have the highest genetic relationship and similarity (80 %).
Agronomic Improvement of Argomulyo Soybean Variety [Glycine max (l) merr] through Induced Mutation by Gamma Irradiation in M1 and M2 Generation Diana Sofia Hanafiah; Trikoesoemaningtyas Trikoesoemaningtyas; Sudirman Yahya; Desta Wirnas
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 27, No 3 (2010)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2010.27.3.199

Abstract

Gamma ray irradiation, especially applied at low doses, is one of methods to produce plant genetic diversity by means of micromutation. The objectives of this research was to determine the radiosensitivity of Argomulyo variety of soybeans (Glycine max (L) Merr) by gamma ray irradiation and to estimate genetic variablity of the crop after  irradiation. To determine the radiosensitivy, the seeds of Argomulyo variety were treated with gamma ray irradiation at  200 Gy, 400 Gy, 600 Gy, 800 Gy, and 1000 Gy. Curve-fit analysis was employed to find the Lethal Doses 50 (LD50) value . To evaluate the genetic variablity after irradiation with gamma ray, seeds of Argomulyo was irradiated under LD50  at doses of 0 Gy, 50 Gy, 100 Gy, 150 Gy, and 200 Gy.  The results showed that LD50 value was 457.17 Gy. The study found that for Argomulyo variety the LD20 was 490.93 Gy and the LD50 was 457.17 Gy.  Genetic variability was observed in various agronomic characters of M2 generation after low dose of gamma ray irradiation treatment of 50, 100, 150 and 200 Gy. The highest genetic variability was found at 200 Gy for plant height, number of productive nodes, and number of pods.  The variability of agronomic characters of soybean after irradiation was due to genetic factors. The highest heritability estimates was observed in plant height and number of productive pods. The succeding generation of putative mutants will be selected for productivity and drought tolerance.
PENGARUH DINAMIKA GUGUR DAUN TERHADAP KERAGAMAN HASIL LATEKS BEBERAPA GENOTIPE KARET HARAPAN HASIL PERSILANGAN 1992 DI PENGUJIAN PLOT PROMOSI Sayurandi Sayurandi; Desta Wirnas; Sekar Woelan
Warta Perkaretan Vol. 36 No. 1 (2017): Volume 36, Nomor 1, Tahun 2017
Publisher : Pusat Penelitian Karet - PT. Riset Perkebunan Nusantara

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (732.636 KB) | DOI: 10.22302/ppk.wp.v36i1.306

Abstract

Tanaman karet menggugurkan daun secara periodik akibat cekaman kekeringan yang terjadi pada bulan-bulan kering setiap tahunnya. Pada umumnya gugur daun di Provinsi Sumatera Utara terjadi pada bulan Januari sampai bulan Maret. Tujuan penelitian ini adalah untuk mengamati keragaman hasil lateks beberapa genotipe karet harapan hasil persilangan 1992 di pengujian Plot Promosiakibat dinamika gugur daun. Penelitian ini dilakukan pada bulan Agustus 2015 – Maret 2016 di Kebun Percobaan Balai Penelitian Sungei Putih, Pusat Penelitian Karet yang terletak di Kabupaten Deli Serdang. Materi genetik yang digunakan pada penelitian ini yaitu sebanyak 15 genotipe karet harapan berasal hasil persilangan 1992 Plot Promosi  dengan 2 klon PB 260 dan RRIC 100 pada umur sebelas tahun. Hasil penelitian menunjukkan bahwa keragaman hasil lateks  dipengaruhi oleh kondisi fase perdaunan  sebagai akibat perubahan pola curah hujan bulanan. Genotipe nomer HP 92/542  memiliki hasil lateks paling tinggi pada semua fase perdaunan, sedangkan paling rendah terdapat pada genotipe HP nomer 92/677 dan HP 92/726. Berdasarkan kondisi fase perdaunan menunjukkan bahwa rerata hasil lateks paling tinggi terdapat pada saat tanaman dalam fase daun penuh. Penurunan hasil lateks secara nyata terjadi pada saat kondisi gugur daun total dan pembentukan daun muda. Persentase penurunan hasil lateks pada kondisi tersebut masing-masing adalah 71,46% dan 73,23% terhadap fase daun penuh.
INTERAKSI GENOTIPE X MUSIM KARAKTER FISIOLOGI LATEKS DAN DAYA HASIL BEBERAPA GENOTIPE KARET HASIL PERSILANGAN TAHUN 1992 Sayurandi Sayurandi; Desta Wirnas; Sekar Woelan
Warta Perkaretan Vol. 36 No. 2 (2017): Volume 36, Nomor 2, Tahun 2017
Publisher : Pusat Penelitian Karet - PT. Riset Perkebunan Nusantara

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (851.313 KB) | DOI: 10.22302/ppk.wp.v36i2.388

Abstract

Latex physiology characters and yield potential of rubber tree  (Hevea brasiliensis) are determined by genetic factor, environments, and genotype x environment interaction. The environmental conditions greatly influence latex physiology characters and latex yield potential are the monthly rainfalls. The objective of the research was to get information about the performance of latex physiology characters and yield potential of some promising rubber genotypes of crossing results in 1992 in dry and wet months period. The experimental design used was randomize completely design (RCD) with two factors and three replications. The first factor was genotypes consisted of 15 genotypes and 2 control clones of PB 260 and RRIC 100. The second factor was season consisted of the wet months period (August - November 2015) and dry months period (January - March 2016) based on classification of Oldeman. The research result showed that the genotype x season interaction effect was significant to sucrose content, inorganic phosphate content, thiol content, latex flow rate, plugging index, yield index, and latex yield potential. Genotype HP 92/542 was a more tolerant to seasonal changes and this genotype had high latex yield and more stable. The genotype can be developed as a new genetic material of superior rubber clone. Genotype HP 92/726 was also tolerant to seasonal changes but the genotype had low latex yield. The genotype HP 92/838 was intolerant to seasonal changes and the genotype had low latex yield and unstable.
Co-Authors ,, Mawaddah . SUDARSONO . SUWARSO Achmad . Adinda Wuriandani Adinda Wuriandani Agung Wahyu Susilo Agung Wahyu Susilo Agung Wahyu Susilo Ahmad Ansori Mattjik Ahmad Ansori Mattjik AHMAD JUNAEDI Aidi Noor Akhmadi, Gerland Alaydi, Nafian Amris Makmur Arvita Netti Sihaloho, Arvita Netti Atika Bakti Sari Azri Kusuma Dewi Bambang Sapta Purwoko Buang Abdullah Dedek hanafiah Dewi Andriani Diana Sofia Hanafiah Didy Sopandie Edi Santosa Edison Jambormias EDIZON JAMBORMIAS Eka Bobby Febrianto, Eka Bobby Eni Widajati Faqih Udin Faradila Median Rini Firman Ardiansyah Gopar, R. A. HAJRIAL ASWIDINNOOR Halimah Tus Sa'diah Halimatus Syahdia Hasibuan HESTI MAULIDA Hidayatun, Nurul Human, dan Soeranto I. Prihantoro, I. Imam Widodo Ishmatalhaq, Zannuba Iskandar Lubis Isnaini Isnaini Iswari S. Dewi Iswari Saraswati Dewi Jaisyurahman, Usamah Jasmi Jekki Irawan Junaedi, dan Ahmad Karlin Agustina Khairil Anwar L. Abdullah Lela Marlenasari M A Chozin Mahpuzah, Marfiatun Manalu, Victor Manotar Pademan Mardhiyyah, Rodhiyatan Marlenasari, Lela Maryono, Marina Yuniawati Mattjik, Ahmad A Maulida, Hesti Mayang Sari Memen Surahman Mizan, Muhammad Rauful Mizan, Muhammad Rauful Momongan, Jorex Daniel Muhamad Syukur Muhammad Arif Yudiarto Munarti Munif Ghulamahdi Nindita, Anggi Nita Kartina, Nita Nofika Senjaya NUR KHOLISOH NURHAJATI AA MATTJIK NURHAJATI MATTJIK Nurheni Wijayanto OTIH ROSTIANA P. D. M. H. Karti Patty, Jacob R Pipit Werdhiwati Prasetiyono, dan Joko Prasetiyono, Joko Pratama, Muhammad Antony Jefri Purbokurniawan . Putra, B. Putri Andini Mandasari Ratih Irma Khairani Saragih Rini, Erin Puspita Rini, Faradila Median S. Human Saniaty, Arina Saragih, Ery Leonardo Saragih, Ratih Irma Khairani Sayurandi Sayurandi Sayurandi Sayurandi Sayurandi Sayurandi Sayurandi Sayurandi, Sayurandi Safitri, Heni Sekar Woelan Sekar Woelan Sekar Woelan Sekar Woelan SESANTI BASUKI SESANTI BASUKI Sherly Rahayu Siti Marwiyah Siti Marwiyah Sobir Sobir Sobir Sobir Sobir Sobir Soeranto Human SRI WARDANI Sudarsono SUDARSONO SUDARSONO Sudarsono, dan Sudirman Yahya Sudirman Yahya Sudirman Yahya Sudirman Yahya - Sumiati, dan Sungkono Sungkono Sunyoto Sunyoto Surjono H Sutjahjo, Surjono H Surjono Hadi Sutjahjo Suwarno, Punjung Medaraji SUWARSO SUWARSO Tasliah, nFN Tias Arlianti Tri Budiyanti Trikoesoemaningtyas Trikoesoemaningtyas Trikoesoemaningtyas Trikosoemaningtyas Trikosoemaningtyas Trikosoemaningtyas Usamah Jaisyurahman W. Puspitasari Wage Ratna Rohaeni WAGE RATNA ROHAENI Wardhani, Azhahara Putri Kusuma Wibawa, Rentang Fajar Cakra Wuriandani, Adinda Yani Nurhadryani Yudiwanti Wahyu E. Kusumo Yulidar Yulidar