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Journal : INDONESIAN JOURNAL OF MEDICAL LABORATORY SCIENCE AND TECHNOLOGY

THE EFFECT OF BEETROOT (Beta vulgaris L.) JUICE ON CHOLINESTERASE ACTIVITY IN FARMERS EXPOSED TO ORGANOPHOSPHATE PESTICIDES Devyana Dyah Wulandari; Andreas Putro Ragil Santoso; Devyani Diah Wulansari
JURNAL INDONESIA DARI ILMU LABORATORIUM MEDIS DAN TEKNOLOGI Vol 1 No 2 (2019): Laboratory Analysis Applied to Medical Properties
Publisher : Universitas Nahdlatul Ulama Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33086/ijmlst.v1i2.1215

Abstract

A decrease in cholinesterase (chE) enzyme activity is an indicator of pesticide poisoning, especially organophosphate pesticides. The chE activity reduction will result in an increase in the amount of acetylcholine and will bound to muscarinic and nicotinic receptors in the central and peripheral nervous system which can lead to the onset of Alzheimer's disease. However, antioxidant products can slow the progression of Alzheimer's disease by protecting neurons from oxidative stress. Red Beetroot (Beta vulgaris L.) contains betalains and phenolic compounds which act as an antioxidants that are capable of preventing such illness. This study aims to prove the concept that antioxidant compounds in red beetroot juice can increase the chE activity in subjects exposed to organophosphate pesticides. This research is an observational study with an experimental approach. This research used 25 farmers who were exposed to organophosphate pesticides as respondents. They were given 500 ml of beetroot juice twice a day for 2 consecutive months. Cholinesterase levels were measured before and after consuming red beet juice. Measurement of cholinesterase levels was carried out using a kinetic photometric test. This method was developed based on recommendations from the German Society of Clinical Chemistry (DGKC). The results showed that the average of chE (U/L) Level Before Treatment was 8.102 and 8.380 after treatment with a p value 0,62. It can be concluded that there was an increase in the level of cholinesterase but it was not statistically significant. This may be caused by a different response to activity of cholinesterase after consuming beetroot juice in each subjects, age difference, smoking habits, and personal protective equipment.
ASSOCIATION BETWEEN TRIGLYCERIDE SERUM LEVELS AND GLOMERULAR FILTRATION RATE (eGFR) IN PATIENTS WITH CHRONIC RENAL FAILURE AT JEMURSARI ISLAMIC HOSPITAL SURABAYA, INDONESIA Uswatun Hasanah; Suhariyadi; Andreas Putro Ragil Santoso
JURNAL INDONESIA DARI ILMU LABORATORIUM MEDIS DAN TEKNOLOGI Vol 2 No 2 (2020): Increasing Research in Diagnostic Laboratory Testing
Publisher : Universitas Nahdlatul Ulama Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33086/ijmlst.v2i2.1668

Abstract

Chronic Renal Failure (CRF) is a progressive and irreversible decrease in kidney function. One risk factor that affects the progression of CRF is dyslipidemia due to abnormalities of lipid metabolisms. Dyslipidemia is characterized by the increase level of total cholesterol, LDL cholesterol, triglycerides, and a decrease in HDL cholesterol level. eGFR is a parameter for assessing the excretion function, by calculating the amount of filtrate produced by the renal glomerulus. This study aims to determine the relationship between serum triglyceride levels with eGFR in patient suffering from CRF at Jemursari Islamic Hospital Surabaya. This type of research is descriptive experimental with cross–sectional design. The study population was the patients with CRF. The primary data in this study was the results of examination of serum triglyceride levels in patients with CRF. The secondary data (serum creatinine levels, body weight, age, and sex) was obtained from medical records of CRF patients in February 2019. As much as 12% of patients were in the 40–50 years old category, 36% of patients were 51–60 years old, 44% of patients were 61–70 years old, and 8% of patients were 71–80 years old. 72% of patients were male. The mean triglyceride level was 146.68 mg/dL and the average eGFR was 19.86 mL/min/1.73m2. The relationships between triglyceride levels and eGFR was carried out by the Spearman–rho test where r value was –0.442 and value was 0.027. It was concluded that there was a significant negative correlation between serum triglyceride levels and eGFR in patients with CRF.
The Relationship between Hemoglobin Levels and Erythrocyte Morphology on the Third Day of Menstruation for Students in The Faculty of Health Azzaubadilluah Azzaubadilluah; Andreas PutroRagil Santoso
JURNAL INDONESIA DARI ILMU LABORATORIUM MEDIS DAN TEKNOLOGI Vol 4 No 1 (2022): The future of diagnostic laboratory testing
Publisher : Universitas Nahdlatul Ulama Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33086/ijmlst.v4i1.2461

Abstract

Menstruation is the process of releasing the endometrial lining of the uterus due to the release of the hormones estrogen and progesterone, causing bleeding after ovulation from the uterus for approximately fourteen days at regular intervals. The first three days of menstruation is a condition where there is heavy bleeding, so there is iron loss, which can cause a decrease in hemoglobin levels and there is a risk of causing anemia. The occurrence of anemia gives an illustration of morphological changes of erythrocytes. The objective of this study was to determine the relationship between hemoglobin levels and erythrocyte morphology on the third day of menstruation. This type of research is analytic observational with a cross-sectional approach. The sample in this study were 30 students from the Health Faculty of Nahdlatul Ulama University Surabaya who experienced menstruation on the third day. The hemoglobin level was analysed using the Hematology Analyzer Xp-300 method. A blood smear test with Giemsa stain were used for abnormalities erythrocytes morphology observation. The results of the statistical analysis used the chi-square test showed there was no relationship between hemoglobin levels and erythrocyte size (p-value = 0.836), erythrocyte shape (p-value = 0.416). However, there was a relationship between hemoglobin levels and erythrocyte colour (p<0.05). Based on the study result, it can be concluded that there is no relationship between hemoglobin levels and erythrocyte morphology on the third day of menstruation.
Co-Authors , Wirda Nur Fa'ida Ahmad Miftahul Kaunain Aishella, Maulida Akhmad Rizal Effendi Alfian Daffa Alfri Danti Izky Oktavia Amalia, Ikke Nanda Andini, Ary Anggriyani, Rinda Ardyarini Dyah Savitri Ardyarini Dyah Savitri Ardyarini Dyah Savitri Arif, Sheila Weni Astuti Arifina, Rosyidatul Arvian Nur Amalina Asror, Anita Rizki Rahmawati Azzaubadilluah Azzaubadilluah Devyana Dyah Wulandari Devyana Dyah Wulandari Devyani Diah Wulansari Devyani Dyah Wulansari Diaz Syafrie Abdillah Dra. Wieke Sri Wulan, S.T., MARS., M. Kes Dwi Handayani Edjianto, Soebagijo Poegoeh Effendi, Akhmad Rizal Ekawati, Evy Ratnasari Endah Budi Permana Putri Endah Budi Permana Putri Ersalina Nidianti Fadlillah Triasmoro Iskandar Filia, Sisca Gilang Nugraha Halimatul Afifah Hanum, Mufida Heris Santy, Wesiana Hotimah Masdan Salim Imratul Mukarramah Janetra Yudhis Nata P Kardina, Rizki Nurmalya Khoir, Intan Wahyunillah Zaidah Khusnah, Hidayatul Lili Susan Rahmawati Ma'at, Suprapto Mardhotillah, Rachma Rizqina Mega Silvia Anggraini Sukri Mella Rahmawati Mukminin Mufida, Nisaul Muhammad Afwan Romdloni Muhammad Taufiq Hidayat Muhammad Taufiq Hidayat Muhammad, Akbar Reza Mukhtar Adi Nugroho Murtasiyah, Erna Dwi Nathalya Dwi Kartika Sari Nesa Emilia Maretha Nila, Hartatiek Nur Izzatul Awaliyah Nur Masruroh Nur Masruroh Nur Masruroh Nur Masruroh, Nur Nur Zalsabila Isnaini Pradana, Muhammad Sungging Pratiwi, Viera Nuriza Prayekti, Endah Putri, Fildzah Karunia Rahayu, Mita Retno Kawuri Rindi Anifa Laloda Rizki Nurmalya Kardina Rohmah, Wardah S, Widya Novida S. P. Edijanto Penulis Salim, Hotimah Masdan Santoso, Setyo Dwi Setiarsih, Dini Suhariyadi Suhariyadi Suprapto Maat Syinta Nur Isnaini Ulan, Della Citra Uswatun Hasanah Widya Novitasari Wijanarko, Robert Aditya Wildani Thoyyibah Wiwi Azlina Syawalien Wulansari, Devyani Diah Wulansari, Devyani Diah Zazabilla, Indira