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All Journal Jurnal Ilmu Pertanian Indonesia AL KAUNIYAH Elkawnie JURNAL ENGGANO BIOLINK (Jurnal Biologi Lingkungan, Industri, Kesehatan) Jurnal Penelitian Pendidikan IPA (JPPIPA) Jurnal Pengabdian Pada Masyarakat Martabe : Jurnal Pengabdian Kepada Masyarakat BIOEDUSAINS: Jurnal Pendidikan Biologi dan Sains Jurnal Mangifera Edu Jurnal Pembelajaran dan Biologi Nukleus Dharma Raflesia : Jurnal Ilmiah Pengembangan dan Penerapan IPTEKS Jurnal Pengabdian kepada Masyarakat Nusantara SN-Biosper Jurnal Pengabdian Pada Masyarakat Bulletin of Chemical Reaction Engineering & Catalysis
Risky Hadi Wibowo
Universitas Bengkulu
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Pemanfaatan Tanaman Obat Keluarga (TOGA) Meningkatkan Imunitas Tubuh sebagai Pencegahan COVID-19 Reza Pertiwi; Doni Notriawan; Risky Hadi Wibowo
DHARMA RAFLESIA Vol 18, No 2 (2020): DESEMBER (ACCREDITED SINTA 5)
Publisher : Universitas Bengkulu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33369/dr.v18i2.12665

Abstract

Pada bulan Desember, 2019, serangkaian kasus pneumonia yang penyebabnya tidak diketahui muncul di Wuhan, Hubei, Cina, dengan gambaran klinis sangat menyerupai virus pneumonia. Setelah dilakukan uji laboratorium menunjukkan adanya virus baru, yang diberi nama COVID-19. Jumlah kasus terus tumbuh secara eksponensial menyebabkan WHO mengingatkan semua negara untuk melakukan kegiatan pencegahan dan pengendalian yang kuat agar dapat menghentikan penyebaran virus ini. Sejauh ini, belum ditemukannya obat spesifik untuk penyembuhan infeksi COVID-19. Terapi yang digunakan hanyalah menghilangkan gejala yang muncul dengan meningkatkan imunitas penderita. Potensi peningkatan imunitas dari jamu dapat diperoleh dari tanaman obat keluarga (TOGA). Tanaman obat dapat meningkatkan kekebalan tubuh, karena bersifat pencegahan (preventif) dan promotif melalui kandungan metabolit sekunder contohnya seperti gingiro pada jahe dan santoriso pada temulawak yang mampu meningkatkan sistem kekebalan tubuh. Berdasarkan observasi mahasiswa UNIB dan masyarakat di Kelurahan Pematang Gubernur, Kecamatan Muara Bangkahulu RT. 22, masih membutuhkan jamu sebagai pencegahan terhadap penyebaran virus COVID-19
Kombinasi Ekstrak Metanol Daun Ketepeng Cina (Cassia alata Linn.) dengan Enam Ekstrak Tumbuhan Terpilih dalam Menghambat Pertumbuhan Bakteri Staphylococcus aureus Lisna Hamni; Avidlyandi Avidlyandi; Charles Banon; Khafit Wiradimafan; Deni Agus Triawan; Risky Hadi Wibowo; Morina Adfa
BIOEDUSAINS:Jurnal Pendidikan Biologi dan Sains Vol 5 No 2 (2022): BIOEDUSAINS:Jurnal Pendidikan Biologi dan Sains
Publisher : Institut Penelitian Matematika, Komputer, Keperawatan, Pendidikan dan Ekonomi (IPM2KPE)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31539/bioedusains.v5i2.3996

Abstract

This study aimed to determine the antibacterial activity and the effect of the combination of methanol extract of the leaves of Chinese ketepeng (Cassia alata Linn.) with six selected plant extracts against Staphylococcus aureus bacteria. The method used is well diffusion and paper tape method. The concentration of the test extract was 100 mg/mL (1:1), DMSO 50% v/v was used as a negative control and the antibiotic clindamycin as a positive control. The results showed that most of the combinations of the methanol extract of the Chinese ketepeng leaf (C. alata) with the six selected plant extracts produced an average diameter of the inhibition zone, which was greater than the average diameter of the inhibition zone of every single extract. However, the average diameter of the combined inhibition zones was not more significant than the sum of the average diameters of the inhibition zones of every single extract. In conclusion, the combination of the methanol extract of the Chinese ketepeng leaf (C. alata) with six selected plant extracts had additive/indifferent effects in inhibiting the growth of S. aureus. Keywords: Antibacterial, Cassia alata Linn, Combination, Staphylococcus aureus
Kemampuan Bakteri Endofit Pelarut Fosfat dari Tumbuhan Akar Kuning (Arcangelisia flava (L.) Merr) Asal Pulau Enggano, Provinsi Bengkulu Risky Hadi Wibowo; Stella Reformanda Sembiring; Sipriyadi Sipriyadi; Welly Darwis; Rochmah Supriyati; Thoriqul Hidayah; Sal Prima Yudha
Al-Kauniyah: Jurnal Biologi Vol 15, No 2 (2022): AL-KAUNIYAH JURNAL BIOLOGI
Publisher : Department of Biology, Faculty of Science and Technology, Syarif Hidayatullah State Islami

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15408/kauniyah.v15i2.17632

Abstract

AbstrakFosfor merupakan salah satu unsur makro nutrien yang dibutuhkan untuk perkembangan tumbuhan dan dapat dihasilkan oleh bakteri pelarut fosfat. Namun, keberadaan fosfat dalam tumbuhan tidak dapat digunakan oleh tumbuhan itu sendiri, sehingga dibutuhkan bantuan bakteri endofit yang berperan sebagai agen pemacu pertumbuhan tanaman yang dapat melarutkan fosfat pada tumbuhan, khususnya tumbuhan akar kuning (Arcangelisia flava (L.) Merr) yang tumbuh liar di Pulau Enggano, Provinsi Bengkulu. Tujuan penelitian ini untuk memperoleh bakteri endofit yang dapat melarutkan fosfat. Isolasi dilakukan dengan metode sterilisasi permukaan, menggunakan alkohol 70% dan natrium hipoklorit 5,25% lalu ditempelkan di media NA. Isolat yang diperoleh kemudian diuji kemampuan melarutkan fosfat dengan digoreskan ke media Pikovskaya, lalu diukur zona bening. Hasil penelitian menunjukkan dari 18 isolat bakteri endofit, 8 di antaranya dapat melarutkan fosfat. Isolat AKE15 dan AKE18 memiliki kemampuan lebih tinggi untuk melarutkan fosfat dengan indeks pelarut fosfat (IP) ± 1,59 dan ± 1,01. Identifikasi berdasarkan pewarnaan Gram dan uji biokimia dari 8 isolat bakteri endofit pelarut fosfat (BPF) memiliki kedekatan dengan 4 genus, yaitu Bacillus, Micrococcus, Pseudomonas, dan Morococcus.AbstrakPhosphorus is one of the macronutrients required for plant development and produced by phosphate solubilizing bacteria. However, plants are unable to utilize phosphate themselves, thus they need the help of endophytic bacteria that play role as plant growth-promoting with ability to solubilize phosphates in plants, particularly in yellow root plants (Arcangelisia flava (L.) Merr) that grow wildly in Enggano Island, Bengkulu Province. The research aimed to obtain endophytic bacteria that can solubilize phosphate. Isolation of endophytic bacteria was carried out by surface sterilization method using 70% alcohol and 5.25% sodium hypochlorite to be further placed on Nutrient Agar (NA) media. The isolates obtained were further tested for their ability to solubilize phosphate by streaking the isolate on Pikovskaya media and measuring the clear zones formed. The results showed that 8 out of 18 endophytic bacterial isolates were able to solubilize phosphate. Endophytic isolates of AKE15 and AKE18 were found to have higher ability to solubilize phosphate with a phosphate solubilizing index (IP) of about ± 1.59 and ± 1.01, respectively. Identification based on Gram staining and biochemical tests indicated that 8 endophytic bacterial solubilizing isolates were closely related to 4 genera, namely Bacillus, Micrococcus, Pseudomonas, and Morococcus. 
Pengenalan dan Cara Mengidentifikasi Makrofungi untuk Menunjang Proses Belajar Mengajar Guru dan Santriwati di Madrasah Aliyah Al-Hasanah, Bengkulu Tengah Welly Darwis; Risky Hadi Wibowo; Helmiyetti Helmiyetti; Reza Wahyuni; Elsi Silvia; Dhea Amelia Sari; Aldi Trianda; Aulia Adriansyah
Jurnal Pengabdian Pada Masyarakat Vol 7 No 1 (2022): Jurnal Pengabdian Pada Masyarakat
Publisher : Universitas Mathla'ul Anwar Banten

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (507.924 KB) | DOI: 10.30653/002.202271.39

Abstract

Information about the introduction and how to identify the macrofungi in the Madrasah Aliyah Al-Hasanah is still low, so it is necessary to introduce methods for dealing with macrofungi. Identification of macrofungi is one of the methods used to determine the macrofungi, whether they are pathogenic or not by looking at the morphological characteristics of the macrofungi. The activity of community service began with licensing by the Madrasah, providing material about macrofungi, the community service team shows some examples of wet herbariums for several previously identified macrofungi. After studying the macrofungi technique, the community service team and students developed skills in finding macrofungi from several wet herbariums that had been provided by the community service team, and formed study groups for participants to better understand the introduction and how to identify macrofungi. In this community service, it is hoped that the participants can develop skills in finding, discovering, identifying and preserving of the macrofungi and become apart of learning material for teachers and students. Macrofungi can also be used as medicine in the health sectors, as an environmental decomposer and useful in the economic field because they can be used as food ingredients.
the Pengenalan Makanan Bernutrisi dan Pemeriksaan Saturasi Oksigen Pada Anak Penyandang Talasemia Santi Nurul Kamilah Kamilah; Choirul Muslim; Fatimatuzzahra; Vestidhia Yunisya Atmaja; Dian Fita Lestari; Sipriyadi; Risky Hadi Wibowo
Jurnal Pengabdian kepada Masyarakat Nusantara Vol. 4 No. 2 (2023): Jurnal Pengabdian kepada Masyarakat Nusantara (JPkMN)
Publisher : Cv. Utility Project Solution

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55338/jpkmn.v4i2.1065

Abstract

The majority of thalassemia patients in Rejang Lebong Bengkulu had dietary issues. Due to their parents' insufficient understanding of thalassemia, some of them do not undergo routine blood transfusions. With the goal to improve parents' understanding of thalassemia and its problems, this community service project was carried out at the Indonesian Thalassemia Parents Association (POPTI) organization in the Rejang Lebong Regency of Bengkulu. It included measuring oxygen saturation and introducing nutritious foods for people with thalassemia. In order to explain thalassemia, the inheritance of thalassemia features, and the introduction of significant nutritious foods for persons with thalassemia, this activity was carried out utilizing the telling method. On 10 thalassemia patients with ages ranging from 2 to 20 years old, the oxygen saturation was assessed using the direct survey method and an oximeter. According to the oximeter, all individuals with thalassemia have normal oxygen saturation levels. Both parents and kids are aware of the value of blood transfusions and a healthy diet for thalassemia. This activity has a good impact on parents and kids to improve parents' knowledge about thalassemia and its issues, the causes of thalassemia and its heredity, children's oxygen saturation, and its meaning. Parents may reduce the risk of health issues in people with halassemia by understanding the reasons for food that needs to be taken and needs to be avoided. Sebagian besar anak penyandang talasemia yang ada di Rejang Lebong Bengkulu mengalami permasalahan gizi. Beberapa diantaranya tidak melakukan transfusi darah rutin akibat pemahaman orang tua yang masih rendah mengenai talasemia. Kegiatan pengabdian kepada masyarakat ini dilakukan pada komunitas Persatuan Orang Tua Penderita Thalassemia Indonesia (POPTI) cabang Bengkulu yang ada di wilayah Kabupaten Rejang Lebong Bengkulu dengan tujuan untuk meningkatkan pemahaman orang tua tentang penyakit talasemia dan permasalahannya melalui pemeriksaan saturasi oksigen dan pengenalan makanan bernutrisi bagi penyandang talasemia. Kegiatan ini dilaksanakan dengan metode ceramah untuk menyampaikan penjelasan tentang talasemia, pewarisan sifat talasemia, pengenalan makanan bernutrisi penting bagi penyandang talasemia. Pemeriksaan saturasi oksigen dilakukan dengan metode survei langsung menggunakan alat oximeter pada 10 penyandang talasemia dengan rentang usia 2-20 tahun. Oximeter menunjukkan bahwa, semua penyandang talasemia memiliki saturasi oksigen dalam kadar normal. Orang tua dan anak telah memahami pentingnya transfusi darah dan makanan bernutrisi yang tepat bagi talasemia. Kegiatan ini telah memberikan manfaat yang baik bagi orang tua dan anak dalam upaya peningkatan wawasan dan pemahaman orang tua tentang penyakit talasemia dan permasalahannya, penyebab munculnya talasemia dan pewarisannya, saturasi oksigen anak serta maknanya. Orang tua dapat memahami alasan suatu makanan perlu dikonsumsi dan perlu dihindari bagi anak penyandang talasemia sehingga dapat mengurangi kemungkinan permasalahan kesehatan yang muncul pada anak penyandang talasemia.
Reduction of 4-nitrophenol Mediated by Silver Nanoparticles Synthesized using Aqueous Leaf Extract of Peronema canescens Salprima Yudha S; Aswin Falahudin; Risky Hadi Wibowo; John Hendri; Dennie Oktrin Wicaksono
Bulletin of Chemical Reaction Engineering & Catalysis 2021: BCREC Volume 16 Issue 2 Year 2021 (June 2021)
Publisher : Masyarakat Katalis Indonesia - Indonesian Catalyst Society (MKICS)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.9767/bcrec.16.2.10426.253-259

Abstract

In this study, we developed an alternative of 4-nitrophenol reduction mediated by silver nanoparticles (AgNPs) which was synthesized using aqueous extract of the Peronema canescens leaf through an eco-friendly approach. The reducing 4-nitrophenol to 4-aminophenol mediated by AgNPS in the presence of sodium borohydride as a hydrogen source proceeded rapidly at room temperature without any additional treatments. The AgNPS synthesis was simple and was carried out under mild conditions. Ultraviolet–visible spectroscopy was performed to examine the properties of the obtained AgNPs, which displayed an absorption peak at 431 nm. A transmission electron microscopy analysis revealed that the AgNPs were spherical in shape and had an average particle size of 19 nm as determined by particle size analysis. Copyright © 2021 by Authors, Published by BCREC Group. This is an open access article under the CC BY-SA License (https://creativecommons.org/licenses/by-sa/4.0). 
Isolation and Screening of Soil Chitinolytic Actinobacteria as the Anti-Fungal Producer of Plant Pathogens Risky Hadi Wibowo; Sipriyadi Sipriyadi; Nisa Rachmania Mubarik; Iman Rusmana; Maggy Thenawidjaya Suhartono
Elkawnie Vol. 6 No. 2 (2020)
Publisher : Faculty of Science and Technology Universitas Islam Negeri Ar-Raniry

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22373/ekw.v6i2.7400

Abstract

Abstract: Chitinolytic actinobacteria are currently more widely used because of their ability as the biological control agents to the pathogenic fungi, especially in horticultural and plantation crops. This research was conducted to obtain isolates of chitinolytic soil actinobacteria from the rhizosphere of the rubber plant (Hevea brasiliensis) area in IPB University. Antifungal activities from these actinobacteria hopely able to inhibit the growth of plant pathogenic fungi in Vitro on chitin agar media. Fusarium oxysporum and Sclerotium rolfsii are used in the inhibition test of chitinolytic actinobacteria. The results successfully obtained 16 isolates of actinobacteria were grew on  Humic Acid Vitamin (HV) agar. It showed that six of 16 actinobacteria isolates were able to produce inhibition zones to the growth of hyphae of pathogenic fungi on potato dextrose agar (PDA). KK-15 and KK-07 isolates were able to produce the largest inhibition percentages in F. oxysporum and S. rolfsii. Based on the chitinolytic index (CI) values, KK-15 and KK-07 isolates produced CI values of 1.25 and 1.5, respectively. The morphological characteristics and Gram staining of both KK-15 and KK-07 isolates are closely relative with Streptomyces sp. Abstrak: Bakteri kitinolitik saat ini banyak diteliti karena kemampuannya sebagai agens pengendali hayati jamur patogen khususnya pada tanaman hortikultura dan perkebunan. Penelitian ini dilakukan untuk mendapatkan isolat-isolat bakteri kitinolitik asal tanah dari bagian perakaran tanaman karet (Hevea brasiliensis) di daerah perkebunan karet Institut Pertanian Bogor (IPB). Aktivitas antifungi dari bakteri ini diharapkan  mampu menghambat pertumbuhan jamur patogen tanaman secara in Vitro pada media agar-agar kitin. Fusarium oxysporum dan Sclerotium rolfsii digunakan dalam uji hambat aktinobakteri kitinolitik. Hasil penelitian memperoleh 16 isolat aktinobakteri berhasil diisolasi dengan menggunakan media agar-agar Humic Acid Vitamin (HV), dan dari 16 isolat tersebut, enam isolat mampu menghasilkan zona hambat terhadap pertumbuhan hifa dari kedua jamur patogen pada media Potato Dextrose Agar (PDA) secara in Vitro. Isolat KK-15 dan KK-07 mampu menghasilkan diameter hambatan terbesar pada jamur F. oxysporum dan S. rolfsii. Berdasarkan nilai index kitinolitik (IK), isolat KK-15 dan KK-07 menghasilkan nilai IK sebesar 1,25 dan 1,5 secara berurutan. Karakteristik morfologi dan pewarnaan Gram dari kedua isolat yaitu KK-15 dan KK-07 memiliki kedekatan dengan kelompok Streptomyces spp.
Potential Antagonistic Interactions of Cassia alata L. Leaf Extract and Commercial Antibiotics against Staphylococcus aureus: An In Vitro Evaluation Oki Rokhim Mawakhid; Putri Ramanda; Samuel Billie Tua Sinaga; Winda Shari; Avidlyandi Avidlyandi; Khafit Wiradimafan; Salprima Yudha S.; Risky Hadi Wibowo; Morina Adfa
Jurnal Penelitian Pendidikan IPA Vol 12 No 5 (2026): In Progress
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v12i5.14500

Abstract

Several in vitro studies have demonstrated that the combination of plant extracts with antibiotics may reduce the minimum inhibitory concentration (MIC) of antibiotics against resistant microorganisms. Interactions between plant extracts and antibiotics can be synergistic, additive, or antagonistic. This study aimed to evaluate the potential interaction between Cassia alata leaves methanol extract and five selected conventional antibiotics in inhibiting the growth of Staphylococcus aureus ATCC 29213. Antibacterial activity was assessed using the well diffusion and paper strip methods. The methanol extract of C. alata leaves, when tested singly at a concentration of 100 mg/mL, produced an average inhibition zone of 11.74 mm. Four antibiotics, namely ciprofloxacin, clindamycin, chloramphenicol, and tetracycline, exhibited stronger antibacterial activity with inhibition zones ranging from 19.56–27.26 mm, whereas co-trimoxazole showed no inhibitory effect. The combination of C. alata leaves methanol extract with ciprofloxacin, clindamycin, chloramphenicol, tetracycline, and cotrimoxazole resulted in inhibition zones of 22.26 ± 0.703 mm, 22.54 ± 1.222 mm, 17.91 ± 1.094 mm, 17.57 ± 0.652 mm, and 9.30 ± 1.180 mm, respectively. The combined treatments have potential antagonistic effects, as the inhibition zones were smaller than the sum of the individual activities of the extract and antibiotics. Therefore, the combination was considered less effective in suppressing growth of S. aureus ATCC 29213
Morphological Diversity of Endophytic Bacteria Peppermint (Mentha Piperita L.) Rangga Ardiansyah; Sartika Wati Dwi Sapitri; Muhammad Naufal Dzakwan; Nisa Muhammad Thalib; Monika Thamara; Lia Putri Maharani; Risky Hadi Wibowo; Sipriyadi Sipriyadi; Welly Darwis
JURNAL PEMBELAJARAN DAN BIOLOGI NUKLEUS Vol 11, No 4: Jurnal Pembelajaran Dan Biologi Nukleus December 2025
Publisher : Universitas Labuhanbatu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36987/jpbn.v11i4.6683

Abstract

Background: The peppermint plant (Mentha piperita L.) is a type of medicinal plant that is widely found in temperate regions, especially Europe, North America and Africa. This plant contains essential oils, menthol, and menthone, menthafuran, sesquiterpenes, triterpenes, flavonoids, carotenoids, tannins and various minerals are also widely contained in peppermint leaf oil (M. piperita L.). The purpose of this study was to isolate and determine the morphological diversity of endophytic bacteria from peppermint plants (M. piperita L.). Methodology: The method used in this study is to take isolation samples, pure culture, characterization and gram staining to determine whether it includes gram-negative or positive bacteria. The results of the study of 12 cultures of endophytic bacterial isolates from plants (M. piperita L.) were taken 10 isolates from each different culture media. Findings: The results of morphological observations of bacterial colonies show diverse characteristics in terms of surface, appearan, ceelevation, edges, and colour. Gram Staining Test of Endophytic Bacterial Isolates from the roots, leaves, and stems of mint plants against endophytic bacteria showed that there were gram-positive and gram-negative bacterial isolates. Then the results of bacterial cell shape morphology have a diversity of Cocobacil, Coccus, and Bacil forms so it can be concluded that endophytic bacterial isolates from peppermint plants (M. piperita L.) have varied morphological diversity. Contribution: Data on the morphological diversity of endophytic bacteria from M. piperita L. provides important basic data for further research on the potential of M. piperita L. endophytic bacteria
Co-Authors Aldi Trianda Alfredi Anis Fadhila Ginting Sinisuka Angga Martias Aswin Falahudin Aulia Adriansyah Avidlyandi, Avidlyandi Charles Banon Choirul Muslim Della Indah Medani Della Indah Medani Dennie Oktrin Wicaksono Dhea Amelia Sari Dian Fita Lestari Elsi Silvia Fatimatuzzahra Gustina Dwi Wulandari Gustina Dwi Wulandari Gustria Ernis Helmiyetti Helmiyetti Hizkia Puspa Pertiwi Iman Rusmana John Hendri Khafit Wiradimafan Lia Putri Maharani Lisna Hamni Maggy Thenawidjaya Suhartono Margareth Hutasoit Monika Thamara Morina Adfa Muhammad Naufal Dzakwan Nadya Rosianti Nadya Rosianti Nanang Sugianto Nisa Muhammad Thalib NISA RACHMANIA MUBARIK Notriawan, Doni Oki Rokhim Mawakhid Putri Ramanda R R. Sri Astuti Rangga Ardiansyah Redo Setiawan Reza Pertiwi Reza Pertiwi Reza Pertiwi Reza Pertiwi Reza Wahyuni Rochmah Supriati Sal Prima Yudha SALPRIMA YUDHA S Salprima Yudha S. Samuel Billie Tua Sinaga Santi Nurul Kamilah Sartika Wati Dwi Sapitri Sipriyadi Stella Reformanda Sembiring Tessa Rianti Malau Thoriqul Hidayah Thoriqul Hidayah Triawan, Deni Agus Vero Neka Emelda Vestidhia Yunisya Atmaja Welly Darwis Welly Darwis Welly Darwis Welly Darwis Welly Darwis Winda Shari Yuni Kristina Serlyani