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All Journal Jurnal Kesehatan Folia Medica Indonesiana Biota JURNAL KESEHATAN LINGKUNGAN (Journal of Environmental Health) Universa Medicina BIOTROPIC The Journal of Tropical Biology Journal of Health Science and Prevention AGRIPEAT AL-HAYAT: Journal of Biology and Applied Biology Biota Scripta Biologica Al-Ard: Jurnal Teknik Lingkungan Jurnal Riset Biologi dan Aplikasinya Indian Journal of Forensic Medicine & Toxicology Nusantara Journal of Community Engagement Global Health Science (GHS) JURNAL BIOLOGI PAPUA Engagement: Jurnal Pengabdian Kepada Masyarakat
Moch. Irfan Hadi
Universitas Islam Negeri Sunan Ampel Surabaya
Religion Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Dentistry Education Engineering Environmental Science Health Professions Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Nursing Public Health Social Sciences Other

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Journal : Biota

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Optimization of PCR Protocol for the Identification of Yeasts Isolated from Apis mellifera Honeycomb based on the ITS rDNA Ayudya Fitri Arifa; Nirmala Fitria Firdhausi; Irul Hidayati; Yuanita Rachmawati; Moch. Irfan Hadi
Biota Vol 8 No 2 (2022): Jurnal Biota 2022
Publisher : Faculty of Science and Technology Universitas Islam Negeri Raden Fatah Palembang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19109/Biota.v8i2.10181

Abstract

Yeast is a microorganism that can be found in honeycomb. Yeast identification is a process to find and identify new species. One of which is molecular identification of yeast with rDNA sequences in the ITS region. Before carrying out molecular identification, it is necessary to optimize yeast DNA amplification methods to obtain good DNA sequences that ease the yeast identification process. The purpose of this study was to discover the optimum PCR (Polymerase Chain Reaction)protocols for the identification of yeasts isolated from Apis mellifera honeycomb based on the ITS rDNA. This study used 3 PCR (Polymerase Chain Reaction) protocols, i.e., from Kanti et al. (2018), Ediningsari (2008), and Maulana (2011). This results study shows that the optimum PCR protocol was from Maulana (2011), which produced clear and whole DNA fragment luminescences.
Co-Authors Abdul Azis Adiningsih, Setyo Aditya Sukma Pawitra Aditya Sukma Prawira Agung, Tri Sunan AGUSTINA, EVA Albarkah, M. Rafly ANDYARINI, ESTI NOVI Annisa Inda Sanabila Asri Sawiji Atiqoh Zummah Ayudya Fitri Arifa Choiriyah, S.Si, Chumaidatul Corie Indria Prasasti Dedi Ananta Purba Dedy Suprayogi Dian Tugu Warsito Taufiq Eko Teguh Pribadi Esti Tyastirin Estri Kusumawati Eva Agustina Eva Agustina, Eva Evi Iriani Natalia Faizah, Hanik Fatih, M. Fachrul Funsu Andiarna Hanik Faizah Ika Mustika Irul Hidayati Izza Mahendra Kartika Dewi Kartika Dewi Khuliyah Candraning Diyanah Linggar Dwi Kinasih M Yusuf Alamudi M. Chusnan Ma'arif M. Yusuf Alamudi Ma’arif, M. Chusnan Mei Lina Fitri Kumalasari Mei Lina Fitri Kumalasari Mei Lina Fitri Kumalasari Mei lina Fitri Kumalasari Mei Lina Fitri Kumalasari Mei Lina Fitri Kumalasari Mirna Widiyanti Mirna Widiyanti Mirna Widiyanti Mirna Widiyanti MISBAKHUL MUNIR Misbakhul Munir Misbakhul Munir Muhamad Ratodi Muhammad F. Musyaffa Muhammad Falikhul Musyaffa' Muhammad Yusuf Alamudi Muhammad Yusuf Alamudi Muhammad Yusuf Alamudi Muhammad Yusuf Alamudi Muhid , Abdul Nadlir Nadlir Nadlir Nadlir, Nadlir Nanang Supriatna Natalia, Evi Iriani Nirmala Fitria Firdhausi Nova Lusiana Nugraha, Dimas Putra Wahyu Nur Maulida Safitri Nusa, Raras Widya Puji Maryono Purba, Dedi Ananta Putra, Riswanda Rangga Risa Purnamasari Rizqiyah, Ziadatur Robayanto Robayanto Sanabila, Annisa Inda Setyo Adiningsih Setyo Adiningsih Sigit Ari Saputro Soedjajadi Keman Sri Hidayati Sri Hidayati Suhardi, Haidar Rafli Putra Syahrul Afifa Ratna Sari Tatag Bagus Prakarsa Teguh Taruna Utama Violando, Wiga Alif Widia Purnamasari Yuanita Rachmawati Yudied Agung Mirasa