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Journal : Medical Journal of Indonesia

Elastase-1 concentration in feces of term and preterm infants aged 0 – 4 months Dharmasetiawani, Nani; Firmansyah, Agus
Medical Journal of Indonesia Vol 12, No 2 (2003): April-June
Publisher : Faculty of Medicine Universitas Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (223.152 KB) | DOI: 10.13181/mji.v12i2.95

Abstract

Fecal elastase-1 concentration reflects exocrine pancreatic function. There have been some reports from Europe, but so far there has not been a report of fecal elastase-1 concentration in Indonesia, especially concerning infants. The aim of this study is to know the concentration of elastase-1 in feces of infants aged 1-120 days as a preliminary report of the study of the ontogeny of pancreatic elastase-1 in term and preterm infants. Fecal elastase-1 were measured from feces of 28 healthy preterm and 34 healthy term infants up to 120 days (4 month) of age. Elastase-1 concentration in infants less than 14 days of age fluctuated below 200 μg/gram feces. At the first day of life 80% preterm and 60% term infants had elastase-1 concentration less than 200 μg/gram feces, and by the age of 7 days 50% preterm and 33% term infants had elastase-1 concentration less than 200 μg/gram feces. After 14 days of age its concentration was more than 200 μg/gram feces, regardless of gestational age. This preliminary study corroborates supported the previous studies that the level of fecal elastase-1 reached normal level after 14 days. Future longitudinal study is needed to know elastase-1 concentration in infants less than 14 days. (Med J Indones 2003; 12: 69-72) Keywords: Fecal elastase-1, Infants, Enzyme-link immunosorbent assay
Effects of realimentation on small intestinal morphology and disaccharidase activity in malnutrition Sprague-Dawley rats Sosrosumihardjo, Rustadi; Firmansyah, Agus; Rasad, Asri; Harjodisastro, Daldiyono; Ridwan, Endi; Wanandi, Septilia I.; Retno, Dwirini
Medical Journal of Indonesia Vol 15, No 4 (2006): October-December
Publisher : Faculty of Medicine Universitas Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (149.582 KB) | DOI: 10.13181/mji.v15i4.238

Abstract

Low birth-weight infant and intrauterine growth retardation are still a health problem, especially in Indonesia due to high prevalence and need to be reduced. Malnutrition in infants are most common occur in low birth-weight infants. Malnutrition in rats resulted in hypotrophic and normoplastic mucosa of the small intestine. The finding was not only showed that small intestine was able to maintain its cell number in condition with restriction nutrient, however also suggested the posibility of epithelial regeneration if given adequate nutrient intake. Did realimentation recover the hypotrophic normoplastic mucosa to normotrophic normoplastic? The study aim to answer that question. Experimental animal study with post test-control group design was performed using 40 male litter of Sprague-Dawley rats, was fed standard chow. The study was divided into phases prenatally-induced malnutrition and continued with phase realimentation. The result of this study is the body weight, mucosal thickness, villus height, cryptus depth, ratio of villus/ crypt, number of villi, protein content, and disaccharidases of rats realimentation group was higher than non-realimentation group, but lower than control group. Prenatally-induced malnutrition did not reduced the population of small intestinal enterocytes. Realimentation in rats in prenatally-induced malnutrition was able to improve the hypotrophy of small intestinal mucosa and to increase the disaccharidases activities but did not reach the normal values. Realimentation in rats in prenatally-induced malnutrition was able to improve the maturity of small intestine mucosa but did not reach the normal values. The information will be helpfull to decide the policy of maternal malnutrition. (Med J Indones 2006; 15:208-16)Keywords: small intestinal morphology, disaccharidase activity, Sprague-Dawley rats, prenatally-induced malnutrition, realimentation.
Quantitative analysis of lactose and lactulose in urine by high performance liquid chromatography for determination of intestinal lactase activity Dharmasetiawani, Nani; Suryadi, Herman; Firmansyah, Agus
Medical Journal of Indonesia Vol 12, No 1 (2003): January-March
Publisher : Faculty of Medicine Universitas Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (231.365 KB) | DOI: 10.13181/mji.v12i1.84

Abstract

Determination of intestinal lactase activity is directly done by measuring its activity in intestinal epithelium. This is an invasive method and ethically can not be done in healthy infants. Indirectly, determination of lactase activity, stated as excretion and ingestion ratio of lactose and lactulose, needs 30 hours hospitalized infants. The aim of this study was to look for a method for determination of lactase activity which is not invasive and not necessary hospitalized. Using this method  lactose and lactulose were given as a single oral load after 2 hours fasting. Urine were collected for 5 hours starting from consuming sugar solution and then lactose and lactulose concentration in the urine were measured by High Performance Liquid Chromatography. The results showed single oral load of lactose and lactulose can be used for determination of lactase activity in infant and the infants were observed only for 7 hours. (Med J Indones 2003; 12: 8-12) Keywords: Lactase activity, Lactulose, Lactose, High Performance Liquid Chromatography
The effect of Bifidobacterium animalis lactis HNO19 supplementation among pregnant and lactating women on interleukin-8 level in breast milk and infant’s gut mucosal integrity Dewanto, Naomi E.F.; Firmansyah, Agus; Sungkar, Ali; Dharmasetiawani, Nani; Sastroasmoro, Sudigdo; Kresno, Siti B.; Suradi, Rulina; Bardosono, Saptawati; Prasetyo, Dwi
Medical Journal of Indonesia Vol 26, No 3 (2017): September
Publisher : Faculty of Medicine Universitas Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (484.297 KB) | DOI: 10.13181/mji.v26i3.1481

Abstract

Background: Newborn’s gut mucosal is not fully developed, therefore infants are prone to diarrhea. Probiotic supplementation is known to induce the gut mucosal maturity. This study aimed to identify whether probiotics supplementation among pregnant women since the third trimester would increase the infant’s gut mucosal integrity.Methods: A double-blind, randomized clinical trial was conducted to understand the potential effect of probiotic supplementation on the level of probiotics and IL-8 in breastmilk, urine IFABP, faecal α-1-antytripsin (AAT) and calprotectin in infant’s at birth (V0) and three-months old (V3). A single strain of Bifidobacterium lactis animalis HNO19 (known as DR10) was used since it was not the resident bacteria. The study was held at Budi Kemuliaan Hospital and its satellite clinics from December 2014 to December 2015.Results: About 14% (5/35) and 20% (7/35) of the subjects had DR10 in the breastmilk’s colostrum and at the age of 3-months. The median values of IL-8 in the probiotic group vs the placebo group at V0 and V3 were 2810,1 pg/mL vs 1516.4 pg/mL (p=0.327) and 173.2 pg/mL vs 132.7 pg/mL (p=0.211) respectively. IFABP level 211.7 ng/mL vs 842.5 ng/mL (p=0.243) and 25.3 ng/mL vs 25.1 ng/mL (p=0.466); AAT 136.2 mg/dL vs 148.1 mg/dL (p=0.466) and 24 mg/mL vs 29.72 mg/mL (p=0.545); Calprotectin 746.8 ng/mL vs 4645.2 ng/mL (p=0.233) and 378.6 ng/mL vs 391.3 ng/mL (p=0.888).Conclusion: Probiotic DR10 given to pregnant women since the 3rd trimester can be found in colostrum and 3-months breastmilk. However, it did not affect the level of other probiotics or IL-8 and the gut mucosal integrity.
Decreased basal and n-Butyrate stimulated colonic water and electrolyte transport in the pre- and postnatal malnourished rat Firmansyah, Agus
Medical Journal of Indonesia Vol 2, No 1 (1993): January-March
Publisher : Faculty of Medicine Universitas Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (2189.313 KB) | DOI: 10.13181/mji.v2i1.3598

Abstract

[no abstract available]