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Journal : Jurnal Sain Veteriner

Screening and Identification of Lactic Acid Bacteria from Livestock Rumen as Probiotic Candidates Widianingrum, Desy Cahya; Tanzil, Ahmad Ilham; Masnilah, Rachmi; Nurcahyanti3, Suhartiningsih Dwi; Fitriani, Vivi; Khasanah, Himmatul
Jurnal Sain Veteriner Vol 43, No 2 (2025): Agustus
Publisher : Faculty of Veterinary Medicine, Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.104307

Abstract

Population of lactic acid bacteria (LAB) in the rumen of fattening livestock is relatively high and has the potential to be utilized. This study aimed to screen and identify LAB from the rumen as probiotic candidates. The research used rumen liquid from cattle, goats, and sheep collected from slaughterhouses in Bondowoso. The screening and identification processes were conducted at the Livestock Agroindustry Laboratory, Faculty of Agriculture, University of Jember. The screening included colony morphology, Gram staining, cell shape, catalase test, and Carbon dioxide (CO2) gas production ability. The final identification of potential probiotic bacterial candidates was based on their bile salt tolerance. The results showed that there were LAB candidates with 22 isolates (from sheep/OS), 19 isolates (from cattle/OC), and 18 isolates (from goats/OG). The bacterial colony shape included oval (2OS, 1OC), circular (11OS, 18OC, 12OG), irregular (7OS, 6OG), and spindle (2OS). Colony sizes ranged from small (0.5–1.2 mm: 7 OS, 9OC, 4OG), medium (1.2–3.0 mm: 7OS, 7OC, 7OG), to large (3.0–5.0 mm: 8OS, 3OC, 7OG), with the majority of colonies being white and cream-colored, and a minority being yellow. All bacteria were Gram-positive, with coccus (19OS, 14OC, 2OG) and bacillus (3OS, 5OC, 16OG) shaped cells. All samples were capable of producing CO2 gas, and 50% of the isolates possessed catalase enzymes. Based on bile salt tolerance tests, 15% of the isolates had a survival rate of over 50%. In conclusion, LAB from the rumen, particularly goats, were identified as potential probiotic candidates for further development.
Co-Authors A.A. Ketut Agung Cahyawan W Abdul Manan Abdul Sakti Agusrinal Agusrinal Ahmad Ilham Tanzil Ahmaliun, La De Aldira, Davina Amanda Aradea Sufla Andri Akhiruddin Lubis Anis Zubaidah Annika Maizeli Azhari, Novia Azizah Azizah Azizah Azizah Bana, Sahindomi Basrudin Basuki , Bhusry Afra Diwanto Bintang Dharma Budiman, Subhan Arif Cahyoadi Bowo Daniati Daniati Delfia Putri Desi Ratnasari DEWI FITRIANI Diana Susanti Diana Susanti, Febriyanti, Ruth Rize Paas Megahati S, Diana Susanti, Diga Mulya Dinda Ayu Candra Kirana Ella Selselviana Elza Safitri Erismar Amri Fahidu, Wa Ode Hastiani Fatahuddin, Muhammad Febri Hartisa Gandri, La Hadjar, Nur Hayati Hasani, Umar Ode Hermiyanto, Bambang Hidayat, Herlan Himmatul Khasanah I Ketut Suada Jasmi Jasmi Kahirun, Kahirun Khasanah, Ulfa Maunatul Khusnul Hayati La Baco S La De Ahmaliun La Ode Midi, La Ode La Ode Muhammad Erif Laia, Despri Evida Laily Mutmainnah Lies Indriyani Liza Yulia Sari Lucyana Deviy Akmal Lufri Lufri Mades Fifendy Maisal, Agung Soleh Mandala, Marga Maya Gustiani Putri Mega Hairani Megahati S Megahati S Megahati Siregar Mhd. Sarip Muhsimin Muhsimin, Muhsimin Mulyati Mulyati Mutia Yunelki Nur Arafah Nurcahyanti3, Suhartiningsih Dwi Nurhadi Pitaloka, Amelia Ayu Pujirahayu, Niken Rachmi Masnilah Razinsyah, Mia Renny Risdawati Rina Nofrika Yanti Romi Julyada RUTH RIZE PAAS MEGAHATI, RUTH RIZE PAAS Sadim Klaida, Fitriani Siska Nerita Siti Riski Amelia Siwi, La Ode Sri Samsundari Sri Samsundari, Sri Sri Wahyuni Syadzli Agus Saputera Teke, Junartin Tika, Sukma Agustin Dyan Tuwu, Eka Rahmatiah Uslinawaty, Zakiah Wati, Gita Seftia Widianingrum, Desy Cahya Wina, Mahrani Wiwit Gusdianti Yudi Angkana Yuni Ahda