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All Journal JURNAL KIMIA SAINS DAN APLIKASI Bioscience AL KAUNIYAH Jurnal Penelitian Pendidikan IPA (JPPIPA) Jurnal Biologi Tropis EKSAKTA : Jurnal Penelitian dan Pembelajaran MIPA Indonesian Food Science and TechnologyJournal Martabe : Jurnal Pengabdian Kepada Masyarakat JURNAL PENDIDIKAN TAMBUSAI Pelita Eksakta Jurnal Biogenerasi Journal of halal product and research (JHPR) 3BIO: Journal of Biological Science, Technology and Management Jurnal Jeumpa Halal Research Journal TROPICAL GENETICS MASALIQ: Jurnal Pendidikan dan Sains Promotor: Jurnal Mahasiswa Kesehatan Masyarakat Tropical Genetics Symbiotic: Journal of Biological Education and Science Jurnal Serambi Biologi
Achyar, Afifatul
Departemen Biologi, Fakultas Matematika Dan Ilmu Pengetahuan Alam, Universitas Negeri Padang, Padang Indonesia
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Journal : Tropical Genetics

 1   2 
Analysis of Genetic Variation of MatK Gene Sequences in Ammothamnus lehmannii NCBI Popset 2440747918 Using In Silico RFLP Amini, Dara Suci; Afifatul Achyar
Tropical Genetics Vol. 3 No. 2 (2023): Genetics
Publisher : Genetikawan Muda Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/tg.v3i2.49

Abstract

Genetic diversity or genetic variation is variation that occurs in an organism due to differences in the sequence of nucleotide bases (adenine, thymine, guanine and cytosine) that form DNA in cells. Variation genetics can be studied in silico using available gene sequences in the NCBI genbank database. This study used the MatK (Maturase-K) gene sequence with the identity number Popset 2440747918 which was downloaded in fasta format from NCBI . Then screening of restriction enzyme candidates was carried out to determine the restriction enzymes prior to in silico RFLP. The restriction enzyme selected from the screening was the restriction enzyme HindIII which has the recognition site A'AGC_T. The results obtained from 79 samples of DNA sequences, 76 samples were cut and 3 samples were not. And found three allele variations with the percentage of the presence of fragments A1 (86.07%), A2 (10.12%) and A3 (3.79%). The percentage values and frequencies of these A1, A2 and A3 alleles indicate a low level of genetic variation.
Analysis of Genetic Variations in bHLH Protein (Rc) Gene Sequences in Rice (Oryza) NCBI PopSet 2496581476 Using In-Silico RFLP: Analisis Variasi Genetik Sekuen Gen Rc-bHLH Protein pada Padi (Oryza) NCBI PopSet 24596581476 Menggunakan RFLP secara In-Silico Hasanah, Nurul; Violita, Violita; Achyar, Afifatul
Tropical Genetics Vol. 3 No. 2 (2023): Genetics
Publisher : Genetikawan Muda Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/tg.v3i2.50

Abstract

Rice is a plant from the Graminae Family that is spread almost throughout the region. In rice, there is an Rc gene that codes for the formation of the basic loop-helix-loop (bHLH) protein, the Rc gene (Rc-bHLH) functions as a transcription of color pigments in brown rice. This research was conducted computationally from several related sites. The study used restriction enzyme BstUI with a side recognition of 5'-CG'CG-3'. The purpose of this study was to analyze the genetic variation of the Rc gene sequence encoding the bHLH protein in NCBI PopSet rice 2496581476 using RFLP in silico. The results showed that there were genetic variations of the Rc-bHLH gene, 2 allele variations were present in 21 rice sequences using the restriction enzyme BstUI.
Analysis of genetic sequence variation in Formicidae NCBI popset 1871743971 using in silico RFLP Pryatna, Muhamad Zacky; Satria, Rijal; Achyar, Afifatul
Tropical Genetics Vol. 4 No. 1 (2024): Genetics
Publisher : Genetikawan Muda Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/tg.v4i1.43

Abstract

In practically every region of the world, with the exception of the poles, ants are a common form of insect. This one organism lives in colonies and cooperates, therefore these insects are referred to as social insects. In an ant colony, three types of castes have their respective roles and functions, namely, there are queen ants, worker ants, and male ants. In one ant species, many colonies live scattered and each of them has different genes that are influenced by environmental factors and other factors. The purpose of this study was to determine and analyze the genetic variation of ants with the WNT-1 gene sequence in NCBI popset 1871743971. This research was conducted in May-June 2023 using the RFLP method in silico. Based on the results of the study, it was found that there were genetic variations in the WNT-1 gene sequence in Formicidae available at NCBI in PopSet 1871743971.
Analysis of genetic variation of the voltage-gated sodium chanel gene sequence in Aedes aegypti NCBI popset (2325355280) using in silico RFLP Putrizalda, Hafizhah; Achyar, Afifatul
Tropical Genetics Vol. 4 No. 1 (2024): Genetics
Publisher : Genetikawan Muda Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/tg.v4i1.52

Abstract

Aedes aegypti is a mosquito that belongs to the Culicinae Culicids family, order Diptera, class Insecta. This mosquito has the potential to transmit dengue hemorrhagic fever (DHF). DHF is a disease characterized by sudden fever, bleeding both in the skin and in the body and can cause shock and death. In-silico is a research approach whose use will be discussed with advances in technology and available databases. This approach is very commonly used in medical and other healthcare settings. The method used is to use the NCBI site, screen restriction enzymes with an insilico web and continue with the benchling site. The results obtained were using the NheI enzyme and producing two alleles namely Allele A1 and Allele A2. The A2 allele dominates because it has a fragment percentage of 63,3%.
Variasi fenotip cadel (disartria) pada populasi mahasiswa Biologi Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Negeri Padang Hambali, Ahmad; Elviana, Alifah Hazelia; Achyar, Afifatul
Tropical Genetics Vol. 5 No. 1 (2025): Genetics
Publisher : Genetikawan Muda Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/tg.v5i1.78

Abstract

Lisp (Dysarthria) is an abnormality in the nervous system that can affect the performance of the articulatory muscles or speech organs. The Hardy-Weinberg Law states that the frequency of genes in a population remains constant from one generation to the next if there are no evolutionary processes such as migration, mutation, natural selection and gene flow. This research aims to analyze the variation in the Lisp (Dysarthria) phenotype in the Biology Faculty student population. Mathematics and Natural Sciences, Padang State University. Data was obtained through an online questionnaire that identified the predominance of Lisp (Dysarthria) (Dysarthria or not Dysarthria). The results of the study showed that Lisp (Dysarthria) was found in a number of students in a proportion 10 people out of a total of 105 respondents. Analysis using the Hardy-Weinberg law shows that the frequency of dominant (p) and recessive (q) alleles. The frequency of the recessive allele (q) which causes the absence of lisp (dysarthria) is around 0.30, while the frequency of the dominant allele (P) is around 0 .7. Based on calculations, the estimated genotype proportions are 49% homozygous dominant (CC), 42% heterozygous (Cc), and 9.6% homozygous recessive (cc). These results indicate that the population of FMIPA UNP biology students is in Hardy-Weinberg equilibrium regarding the characteristic of lisp (dysarthria). Phenotypic variations of Lisp (Dysarthria) provide an overview of genetic diversity in the population environment of Biology students, Faculty of Mathematics and Natural Sciences, Padang State University and can be the basis for further research regarding the relationship between genetic factors and phenotypic expression in human populations. Keywords: Disartria, Phenotype, Variation, Student, Genetics. ABSTRAK Cadel (Disartria) merupakan kondisi kelainan pada sistem saraf yang dapat memengaruhi kinerja otot artikulator atau organ bicara. Hukum Hardy-Weinberg menyatakan frekuensi gen dalam suatu populasi tetap konstan dari satu generasi ke generasi berikutnya jika tidak ada proses evolusi seperti migrasi, mutasi, seleksi alam dan aliran gen.Penelitian ini bertujuan untuk menganalisis variasi fenotip Cadel (Disartria) pada populasi mahasiswa Biologi Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Negeri Padang.Data diperoleh melalui kuisioner daring yang mengidentifikasi dominasi Cadel(Disartria) (Disartria atau tidak Disartria).Hasil penelitian menunjukkan bahwa Cadel (Disartria) ditemukan pada sejumlah mahasiswa dengan proporsi 10 orang dari jumlah total responden 105 orang. Analisis menggunakan hukum Hardy-Weinberg menunjukkan bahwa Frekuensi alel dominan (p) dan resesif (q).frekuensi alel resesif (q) yang menyebabkan tidak adanya Cadel (Disartria) adalah sekitar 0,30, sedangkan frekuensi alel dominan (P) adalah sekitar 0,7. Berdasarkan perhitungan, proporsi genotip yang diestimasi adalah 49% homozigot dominan (CC), 42% heterozigot (Cc), dan 9,6% homozigot resesif (cc). Hasil ini mengindikasikan bahwa populasi mahasiswa biologi FMIPA UNP berada dalam keseimbangan Hardy-Weinberg terkait sifat Cadel (Disartria) . Variasi fenotip Cadel (Disartria) memberikan gambaran keberagaman genetik di lingkungan populasi mahasiswa Biologi Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Negeri Padang dan dapat menjadi dasar untuk penelitian lebih lanjut mengenai hubungan antara faktor genetik dan ekspresi fenotipik pada populasi manusia. Kata kunci: Disartria, Fenotip, Variasi, Mahasiswa, Genetika.
Variasi Fenotip Lesung Pipit pada Populasi Mahasiswa Biologi Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Negeri Padang Ara, Farrah Azzahra; Suryani, Elisa; Achyar, Afifatul
Tropical Genetics Vol. 5 No. 1 (2025): Genetics
Publisher : Genetikawan Muda Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/tg.v5i1.80

Abstract

Lesung pipi merupakan salah satu variasi fenotip yang mudah diamati pada manusia dan dipengaruhi oleh faktor genetik serta non-genetik. Penelitian ini bertujuan untuk menganalisis variasi fenotip lesung pipit pada populasi mahasiswa Biologi Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Negeri Padang. Metode penelitian ini bersifat deskriptif dengan pendekatan survei. Data dikumpulkan melalui pengamatan langsung dan pencatatan karakteristik lesung pipit, meliputi keberadaannya. Hasil penelitian menunjukkan bahwa lesung pipit ditemukan pada sejumlah mahasiswa dengan proporsi 25 orang dari jumlah total responden 105 orang. Analisis menggunakan hukum Hardy-Weinberg menunjukkan bahwa frekuensi alel resesif (d) yang menyebabkan tidak adanya lesung pipi adalah sekitar 0,48, sedangkan frekuensi alel dominan (D) adalah sekitar 0,52. Berdasarkan perhitungan, proporsi genotip yang diestimasi adalah 27,04% homozigot dominan (DD), 49,92% heterozigot (Dd), dan 23,04% homozigot resesif (dd). Hasil ini mengindikasikan bahwa populasi mahasiswa biologi FMIPA UNP berada dalam kesetimbangan Hardy-Weinberg terkait sifat lesung pipi. Variasi fenotip lesung pipit memberikan gambaran keberagaman genetik di lingkungan populasi mahasiswa Biologi Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Negeri Padang dan dapat menjadi dasar untuk penelitian lebih lanjut mengenai hubungan antara faktor genetik dan ekspresi fenotipik pada populasi manusia.
Analisis Filogenetik Bakteri Escherichia coli Berdasarkan DNA Genomik Gen BlaTEM Dari Data Sekuens NCBI: Pendekatan Bioinformatika terhadap Variasi Gen BlaTEM pada Escherichia coli Tahun 2016–2025 Palupi, Indria; Achyar, Afifatul
Tropical Genetics Vol. 5 No. 2 (2025): Genetics
Publisher : Genetikawan Muda Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/tg.v5i2.102

Abstract

Resistensi antibiotik yang disebabkan oleh bakteri penghasil β-laktamase, seperti Escherichia coli, menjadi permasalahan global yang serius. Gen blaTEM merupakan salah satu gen utama yang berkontribusi terhadap resistensi tersebut. Penelitian ini bertujuan untuk menganalisis hubungan filogenetik antar isolat Escherichia coli berdasarkan sekuens DNA gen blaTEM yang diambil dari database NCBI dalam rentang waktu 2016 hingga 2025. Data sekuens diselaraskan menggunakan metode ClustalW dalam perangkat lunak MEGA X, kemudian dianalisis menggunakan metode Neighbor-Joining dan 1000 bootstrap. Hasil analisis menunjukkan pembentukan beberapa klade utama dengan tingkat kedekatan genetik yang bervariasi. Kelompok isolat tahun 2021–2024 memiliki kedekatan genetik tinggi, sedangkan isolat tahun 2020 dan 2025 menunjukkan divergensi yang signifikan. Temuan ini mengindikasikan adanya variasi evolusioner dalam gen blaTEM yang penting untuk dipantau sebagai bagian dari strategi pengendalian resistensi antibiotik.
Co-Authors Afionita, Santi Ahmad Hambali, Ahmad Ahmad Wibisana, Ahmad Alvenaya Hindayageni Ananda Putri, Ananda Annisa Irna Putri Annisa Khaira Annisa Khaira Annisa, Silvy Aprilia, Amanda Ara, Farrah Azzahra Ardi Ardi Atifah, Yusni Aura Zahra Nafisah Azizah, Jalilah Bintang Fadhil Ramadhan Cici Mustika cynthia perdana putri Dara Suci Amini, Dara Suci Des M Dessy Arisanty Dezi Handayani Dezi Handayani Dina Sukma Dwi Hilda Putri Dwi Hilda Putri Dwi Hilda Putri Edwin Edwin Elsa Badriyya Elsa Yuniarti Elviana, Alifah Hazelia Fadhila Humaira Fardilla, Midratul Fatiha, Fathma Dwi Fatma Sri Wahyuni Fevria, Resti Fitri, Afifah Ismu Fronica, Imelda Gilang Amanda Hafizah Fadhilah Hafizh Alza Afra Helsa Rahmatika Herisanti, Dini Irma Leilani Eka Putri Iyan Robiansyah Iyan Robiansyah Jalilah Azizah Jumatul Hafsah Kardiman, Reki Khairani, Fidia Aura Khairani, Salsabila Putri Linda Advinda Marten, Threo Wanda Monica , Indiastri P Moralita Chatri Muhammad Farikh Muharani, Silvia Mukhlis Mukhlis Mutia Andini, Tri Nabilah, Rezi Nadira Nafisa Arini Nella Fauziah Novita, Yeni Nur Aqsha Nurfadillatun Nisa Wijaya Nurul Hasanah NURUL HIDAYAH Oliv Nurul Kanaya Nurul Kanaya Palupi, Indria Pinta, Sari Rahma Pradila, Andini Novalia Pramila, Cindy Pratama, Chelsylia Dara Pratama, Sandi Fransisco Pryatna, Muhamad Zacky Putri, Aulia Devani Putri, Irma Leilani Putri, Isna Aryunita Putri Putri, Santi Diana Putrizalda, Hafizhah Rahmad Wanizal Pastha Rahmawati, Atika Ayu Rahmi Holinesti Rezeki Rival Alridho Rezi Nabilah Ria Fernanda, Irma Rijal Satria Rijal Satria Rini Wulandari Rinti Mutiara Sari Riza Umami Roza, Sri Yenica Ruri Fitriyani Ruri Fitriyani S. Syamsurizal safitri, fira Salsabil, Velina Salsabilla, Vishtari Sari Rahma Pinta Sari, Rinti Mutiara Satria, Rijal Sefina, Nadia Selaras, Ganda Hijrah Sisca Alicia Farma Suryani, Elisa Vauzia Vauzia Vauzia, Vauzia Velina Salsabil Violita Violita Violita Violita Violita Violita Violita Yulita, Nelfi Yuni Ahda Yuni Ahda Zolkapli Eshak Zultsatunni’mah Zultsatunni’mah Zulyusri Zulyusri Zulyusri Zulyusri Zulyusri, Zulyusri Zulzusri, Zulzusri