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Journal : JURNAL FARMASI DAN ILMU KEFARMASIAN INDONESIA

Antioxidant Properties of Various Yacon Leaf Water Extracts and Physicochemical Profile of Decoction During Refrigerated Storage Wicaksana, Firman; Hartanti, Dwi; Hamad, Alwani
JURNAL FARMASI DAN ILMU KEFARMASIAN INDONESIA Vol. 11 No. 2 (2024): JURNAL FARMASI DAN ILMU KEFARMASIAN INDONESIA
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jfiki.v11i22024.128-136

Abstract

Background: Yacon (Smallanthus sonchifolius (Poepp.) H.Rob.) leaves show promising antioxidant properties, and have traditionally been  used for diabetes management in Baturraden, Banyumas, Central Java, Indonesia. This study evaluated the effects of traditional extraction methods and crude drug-to-solvent ratios on the content and activity of antioxidants and physicochemical properties of yacon leaf water extracts during storage. Methods: Crude drugs were extracted by infusion, short-time decoction, and longer-time decoction at ratios of 1:10, 1:20, and 1:100 (w/v). Antioxidant content was analyzed using standard total flavonoid content (TFC) and total phenolic content (TPC). The antioxidant activity was evaluated using 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging activity and ferric-reducing antioxidant power (FRAP) assays. Yacon leaf water extracts at a ratio of 1:20 (w/v) were stored in tightly closed bottles at 4±2°C for 26 days. The organoleptic characteristics, color, pH, TFC, TPC, and DPPH scavenging activity were evaluated on days 0,1, 3, 6, 12, 18, and 26. Results: The different methods and crude drug-to-water ratios generated different antioxidant activities and contents of the yacon extracts. Yacon leaf decoction for 15 min in a ratio of 1:20 (w/v) produced extract with the best scavenging activity (450.27±5.48 mM Trolox equivalent (TE)/100 g dry weight (DW)), TFC (6.43±0.18 mg Quercetin equivalent (QE)/g DW), and TPC (3.91±0.04 mg gallic acid equivalent (GAE)/g DW). The yacon leaf decoction started to undergo aroma and PH changes on days 3 and 6, respectively. On day 12, the TFC, TPC, and DPPH SA of yacon leaf decoction remained 93.93±3.70, 96.52±1.81, and 89.99±0.91% of the freshly prepared extract, respectively. Conclusion: Our results suggest that extraction using the decoction method for 15 min at a water-to-crude drug ratio of 1:20 (w/v) generated an extract with the best antioxidant profile, which chemically started to change on day 12 during refrigerated storage.
Co-Authors Abdul Haris Mulyadi Afifah, Dini Nur Afwa Hayuningtyas Afwa Hayuningtyas Amiratus Sholekhah Andi Ghina Septhea Andi Ghina Septhea Andi Ghina Septhea, Andi Ghina Anwar Ma'ruf Anwar Ma’ruf Anwar Ma’ruf Ardi Wiranata Arianingsih, Elfa Asmiyenti D. Djalil Asmiyenti Djaliasrin Djalil Asmiyenti Djalisrin Djali Bekti Wulan Sari Bekti Wulan Sari Bekti Wulan Sari Betty Ika Hidayah Charisma, Shintia Lintang Dea Y. Pradani Denia Awanda Putri Denia Awanda Putri Desiana Pradiyanti Dianita Yulia Sukma Dewi Dodi Siraj Muamar Zain Dwi Hartanti Dwi Hartanti Dwi Hartanti Dwi Hartanti Dwi Hartanti Dwi Hartanti Dwi Hartanti Dwi Hartanti Dwi Nugraheni Eka Yuliani Saputri Eka Yuliani Saputri Eli Nurlaeli Eli Nurlaeli Eli Nurlaeli, Eli Endar Pusapawiningtyas Endar Puspawiningtyas Endar Puspawiningtyas Endar Puspawiningtyas Endar Puspawiningtyas Endar Puspawiningtyas, Endar Fadlilah, Intan Nur Fitri Atyani, Fitri Fitriyani Fitriyani Ganda Kurniawan Ganda Kurniawan, Ganda Giswantara Giswantara Haq, M Tsabit Sofatul Haryanto, Totok Haryo Wibisono Haryo Wibisono Hasanah, Yeti Rusmiati Heru Sutopo Heru Sutopo Heru Sutopo Hidayah Anisa Fitri Hierro Azi Priawan Hierro Azi Priawan Intan Nur Fadlilah Istifah Istifah, Istifah Jeri Rinawati Kristiono Kristiono M. Gigih Panji Mahardika, M. Gigih Panji Ma'ruf, Anwar Magribi, Imam Maulana Mauliana, Dinda Mubshair Naveed Mubshair Naveed Mubshair Naveed Muliastri Mentari Naveed, Mubshair Neni Damajanti, Neni Noni Indriani Nur Afifah Andriyani Nur Afifah Andriyani Nur Afifah Handayani Nur Afifah Handayani, Nur Afifah Nur Annisa Septiyaningrum Nur Yulianingsih Nurul Fadhilah Deni Saputri Nurul Fadhilah Deni Saputri, Nurul Fadhilah Deni Pinyapat Jitphongsaikul Pinyapat Jitphongsaikul Pinyapat Jitphongsaikul Pradani, Dea Yulinestria Puspitasari, Adinda Putri, Regita Nanda Regawa Bayu Pamungkas Rinawati, Jeri Rizky Destya Sary Santika, Dita Santosa, Arif Prashadi Septian Chandra Sasmita Shintia Lintang Charisma Shintia Lintang Charisma Sintia Jumitera Suparman Suparman Syah Putri, Tina Syarifah, Arini Tina Syah Putri Tri Nur Chasanah Vishal Chherti Wahyu Utaminingrum Wardani, Tri Kusuma Wicaksana, Firman Widya Agustina Widya Agustina Widya Ayu Dianata Wiwin Anggraeni Yulianingsih, Nur Yulianti , Henti