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RESISTENSI Eleusine indica TERHADAP GLIFOSAT PADA PERKEBUNAN KELAPA SAWIT DI KABUPATEN BATU BARA Koko Tampubolon; Edison Purba; Diana Sofia Hanafiah
Jurnal Agrotek Tropika Vol 6, No 3 (2018)
Publisher : Departement of Agrotechnology, Agriculture Faculty, Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (326.319 KB) | DOI: 10.23960/jat.v6i3.2798

Abstract

Populasi Eleusine indica dari perkebunan kelapa sawit di Kabupaten Batu Bara secara menyeluruh belum pernah dilaporkan resisten-glifosat. Penelitian ini bertujuan untuk melaporkan sebaran populasi dan mengklasifikasi resistensi E. indica terhadap herbisida glifosat pada perkebunan kelapa sawit di Kabupaten Batu Bara. Penelitian ini dilaksanakan di Lahan Pusat Penelitian Gulma Fakultas Pertanian Universitas Sumatera Utara pada Oktober 2016 - Agustus 2017. Penelitian ini menggunakan glifosat dengan dosis rekomendasi 720 g b.a.ha-1 dan tiga ulangan. Populasi ESU0 (dari Lapangan Bola Politeknik Negeri Medan) sebagai pembanding. Analisis data menggunakan software IBM SPSS Statistics 20. Hasil penelitian menunjukkan bahwa populasi E. indica yang paling resisten (63,33%) pada perkebunan kelapa sawit di Kabupaten Batu Bara terdapat pada ESU4.11 (afdeling 4 Kebun Tanah Gambus). Populasi E. indica yang tergolong resisten-glifosat 83,33% (10 populasi), tergolong moderat resisten-glifosat 16,67% (2 populasi) dan tidak ada populasi yang sensitif-glifosat pada dosis rekomendasi 720 g b.a.ha-1 pada perkebunan kelapa sawit di Kabupaten Batu Bara.
Chromosome status and yield characteristics of soybean (Glycine max L. (Merr)) in saline soil as affected by induced mutation Agus Pernando Simanjuntak; Diana Sofia Hanafiah; Luthfi Aziz Mahmud Siregar
Jurnal Ilmu Pertanian Vol 6, No 2 (2021): August
Publisher : Faculty of Agriculture, Universitas Gadjah Mada jointly with PISPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ipas.63621

Abstract

To fulfill the demand of soybeans, the expansion of the planting area can be one of the choices through the conversion of marginal land by utilizing suboptimal land, such as saline soil. One of the plant breeding techniques to obtain tolerant plants to salinity is the use of chemical mutations, such as colchicine. The study aimed to reveal the effect of salinity on the characters of soybean plants (mutated with colchicine) and the effect of colchicine on the number of chromosomes and ploidy level. This study was conducted from January to March 2020 and carried out in the plastic house of Faculty of Agriculture, Universitas Sumatera Utara. The experiment was arranged in a Randomized Block Design with salinity levels (0 dS.m-1, 2 dS.m-1, 4 dS.m-1, 6 dS.m-1) as treatments and consisted of ten replications in each treatment. The result showed that the soybean plants were not successfully induced by colchicine at a concentration of 0.04 % with soaking duration of 10 hours, as indicated by the number of chromosomes and analysis of ploidy level. The soybeans that had been induced were planted in saline soil. There were significant effects observed on the productive branch, number of filled pods, and the weight of seeds per plant. Colchicine treatment did not successfully affect the number of chromosomes of soybeans but had an impact on the chromosome pattern. In addition, the salinity treatment of 4 dS.m-1 significantly affected the number of filled pods compared to the treatment of 6 dS.m-1.
Sebaran Populasi dan Klasifikasi Resistensi Eleusine indica terhadap Glifosat pada Perkebunan Kelapa Sawit di Kabupaten Deli Serdang Koko Tampubolon; Edison Purba; Diana Sofia Hanafiah; Mohammad Basyuni
Caraka Tani: Journal of Sustainable Agriculture Vol 33, No 2 (2018): October
Publisher : Universitas Sebelas Maret

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (45.81 KB) | DOI: 10.20961/carakatani.v33i2.24300

Abstract

Eleusine indica populations on oil palm plantations in Deli Serdang Regency have not been reported to be resistant to glyphosate. This study was aimed to report distribution and resistance classification of E. indica population on oil palm plantations in Deli Serdang Regency. The research was conducted on Weed Research Center in Faculty of Agriculture, Universitas Sumatera Utara from October 2016 until August 2017. This research used Randomized Block Design with recommended dose at 720 g a.i.ha-1 of glyphosate and three replications. Population ESU0 (from Politeknik Negeri Medan Ball Field) as a comparison. Data on average survival, mortality, dry weight, resistance classification of E. indica were analyzeds using IBM SPSS Statistics 20 software. The results showed that there were 12 E. indica populations classified as glyphosate-resistant (56.52%), 7 glyphosate-resistant developing (30.43%), and 4 populations classified as glyphosate-susceptible (13.04%) with the recommended dose at 720 g a.i.ha-1 from oil palm plantations in Deli Serdang Regency.
Agronomic Improvement of Argomulyo Soybean Variety [Glycine max (l) merr] through Induced Mutation by Gamma Irradiation in M1 and M2 Generation Diana Sofia Hanafiah; Trikoesoemaningtyas Trikoesoemaningtyas; Sudirman Yahya; Desta Wirnas
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 27, No 3 (2010)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2010.27.3.199

Abstract

Gamma ray irradiation, especially applied at low doses, is one of methods to produce plant genetic diversity by means of micromutation. The objectives of this research was to determine the radiosensitivity of Argomulyo variety of soybeans (Glycine max (L) Merr) by gamma ray irradiation and to estimate genetic variablity of the crop after  irradiation. To determine the radiosensitivy, the seeds of Argomulyo variety were treated with gamma ray irradiation at  200 Gy, 400 Gy, 600 Gy, 800 Gy, and 1000 Gy. Curve-fit analysis was employed to find the Lethal Doses 50 (LD50) value . To evaluate the genetic variablity after irradiation with gamma ray, seeds of Argomulyo was irradiated under LD50  at doses of 0 Gy, 50 Gy, 100 Gy, 150 Gy, and 200 Gy.  The results showed that LD50 value was 457.17 Gy. The study found that for Argomulyo variety the LD20 was 490.93 Gy and the LD50 was 457.17 Gy.  Genetic variability was observed in various agronomic characters of M2 generation after low dose of gamma ray irradiation treatment of 50, 100, 150 and 200 Gy. The highest genetic variability was found at 200 Gy for plant height, number of productive nodes, and number of pods.  The variability of agronomic characters of soybean after irradiation was due to genetic factors. The highest heritability estimates was observed in plant height and number of productive pods. The succeding generation of putative mutants will be selected for productivity and drought tolerance.
UJI POLIMORPIK DAN HETEROZIGOSITAS PADA PROGENI F4 KEDELAI (Glycine max (L.) Merril) TAHAN SALIN DENGAN MENGGUNAKAN MARKA SSR (Simple Sequence Repeats) Evi Julianita Harahap; Rosmayanti Rosmayanti; Diana Sofia Hanafiah
Jurnal Agrotek Lestari Vol 5, No 2 (2019): Oktober
Publisher : Universitas Teuku Umar

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (324.409 KB) | DOI: 10.35308/jal.v5i2.2229

Abstract

SSR marker has some merits such as quickness, simplicity, rich polymorphism and stability, thus being widely applied in genetic diversity analysis, molecular map construction and gene mapping. the purpose of this study was to determine polymorpic test and heterozygosity in F4 soybean (Glycine max (L.) Merril) progeni saline resistant characters using SSR (Simple Sequence Repeats) markers. This research was conducted in Biomolecular Laboratory, Socfindo Seed Production Laboratory (SSPL), Kebun Bangun Bandar Village Martebing District Dolok Masihul Regency Serdang Bedagai on December-May 2017. The number of samples were used 44. The five SSR primers (QS080465, QS1101, QS1112, QS100011, and Sat_091) used were specific primers, with a band pattern that was clearly visible around one or two bands. The percentage of polymorphic primers (PLP) of these three populations is high, all populations with a PLP of 100% of the saline resistant character. The effective allele number (Ne) of  7,160 for the progeny population is lower than the number of observed alleles (Na) of 10,000 which means that many progeny individuals are homozygous. The expected heterozygosity (He) value of 0.854 in the progeny population was higher than the observed heterozygosity (Ho) value of 0.027. The overall average observed heterozygosity (Ho) was 0.009 lower than the overall expected heterozygosity (He) of 0.61. This means that each character has a low heterozygosity.Keywords: Progeny F4, soybean, SSR, saline resistant, polymorphic, heterozygosity
KERAGAAN GENERASI KETIGA (M3) KEDELAI HASIL IRADIASI SINAR GAMMA PADA KONDISI OPTIMUM DAN KONDISI CEKAMAN KEKERINGAN Diana Sofia Hanafiah
Jurnal Pertanian Tropik Vol. 2 No. 1 (2015): JURNAL PERTANIAN TROPIK
Publisher : Talenta Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32734/jpt.v2i1.2877

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The objective of this research was to obtain a genotype of selected putative mutant withgood agronomic traits and high yielding on the optimum condition and its adaptability todrought stress in M3 generation. The study result showed that there were differences in thegrowth and production of each population as a result of irradiation in optimum conditions anddrought stress conditions. The population as a result of irradiation at 150 Gy and 200 Gy had a highermean value for the plant height trait, the number of productive branches, the number of productivenodes and the number of productive pods compared to irradiation population of 50 Gy, 100 Gy andthe control population of Argomulyo at optimum conditions. The decrease in the growth anddevelopment happened to all the characters observed in the irradiation population in droughtconditions compared to when grown in optimum conditions. The decrease can be seen in thecharacters of plant height, the number of pods and seed weight per plant. The growth and developmentof the population irradiation of 150 Gy and 200 Gy were more influenced by drought conditionscompared to other irradiation populations. The selection in the optimum conditions produced 291high yielding genotypes and the selection in drought conditions generated 202 genotypes andadapted to drought environments.
DISTRIBUTION MAPPING OF GLYPHOSATE-RESISTANT Eleusine indica IN SERDANG BEDAGAI REGENCY Koko Tampubolon; Edison Purba; Mohammad Basyuni; Diana Sofia Hanafiah
Jurnal Natural Volume 18, Number 3, October 2018
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (604.533 KB) | DOI: 10.24815/jn.v18i3.11201

Abstract

The presence of Eleusine indica from oil palm plantations in Serdang Bedagai Regency has never been overall reported glyphosate-resistant. This study aims to distribution mapping and resistance classification of E. indica population to glyphosate herbicide of oil palm plantations in Serdang Bedagai Regency. This research was conducted on Weed Research Center Land in Faculty of Agriculture, Universitas Sumatera Utara in October 2016 to August 2017. This research used glyphosate herbicide with the recommended dose at 720 g ai ha-1 and three replications. Population ESU0 (from Politeknik Negeri Medan Ball Field) as a comparison. Data analysis using IBM SPSS Statistics 20 software. The results showed there E. indica population classified as glyphosate-resistant amount 89.36% (42 population), classified as glyphosate-resistant moderate amount 10,64% (5 population) and there is no population glyphosate-susceptible on oil palm plantations in Serdang Bedagai Regency of recommended dose at 720 g ai ha-1.
DISTRIBUTION MAPPING OF GLYPHOSATE-RESISTANT Eleusine indica IN SERDANG BEDAGAI REGENCY Koko Tampubolon; Edison Purba; Mohammad Basyuni; Diana Sofia Hanafiah
Jurnal Natural Volume 18, Number 3, October 2018
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24815/jn.v18i3.11201

Abstract

The presence of Eleusine indica from oil palm plantations in Serdang Bedagai Regency has never been overall reported glyphosate-resistant. This study aims to distribution mapping and resistance classification of E. indica population to glyphosate herbicide of oil palm plantations in Serdang Bedagai Regency. This research was conducted on Weed Research Center Land in Faculty of Agriculture, Universitas Sumatera Utara in October 2016 to August 2017. This research used glyphosate herbicide with the recommended dose at 720 g ai ha-1 and three replications. Population ESU0 (from Politeknik Negeri Medan Ball Field) as a comparison. Data analysis using IBM SPSS Statistics 20 software. The results showed there E. indica population classified as glyphosate-resistant amount 89.36% (42 population), classified as glyphosate-resistant moderate amount 10,64% (5 population) and there is no population glyphosate-susceptible on oil palm plantations in Serdang Bedagai Regency of recommended dose at 720 g ai ha-1.
A STUDY OF HYDROPONIC MELON CULTIVATIONS WITH SEVERAL SUBSTRATE MEDIA AND VARIETIES Christy, Julieta; Putri, Lollie Agustina P.; Hanafiah, Diana Sofia
Journal of Community Research and Service Vol 1, No 2: July 2017
Publisher : Universitas Negeri Medan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24114/jcrs.v1i2.9343

Abstract

AbstractMelon is a fruit plant that has a high nutritional value. A limited agricultural land encourages the application of alternatives planting method and utilizes local materials around the community as potential planting media towards resulting a high-yielding variety. One of the cultivation techniques is the use of the hydroponics system. The objective of this study is to determine the most suitable planting medium for melon varieties to be planted hydroponically. This research was conducted in the greenhouse at the sub-district of Medan Tuntungan in the city of Medan during May to August 2017. This research used Randomize Complete Block Design Factorial with 3 replications. The use of planting media consisted of charcoal husk, cocopeat, sawdust and sand; whereby the treatment of varieties consisted of Aramis F1, Amanta F1 and Red Aroma. The results showed that the best medium for hydroponics melon is charcoal husk highlighting significant result onto the plant height, number of leaves and fruit weight of crops, from the use of charcoal husk media.Keywords: hydroponic, soilless culture, melon, medium and variety
INCREASING THE PRODUCTIVITY OF A FEW VARIETIES OF ONION (ALLIUM ASCALONICUM L.) WITH THE APLICATION OF AMELIORANT Luta, Devi Andriani; Hanafiah, Diana Sofia; Sabrina, T.
Journal of Community Research and Service Vol 1, No 2: July 2017
Publisher : Universitas Negeri Medan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24114/jcrs.v1i2.9335

Abstract

AbstractThe Onion (Allium ascalonicum L.) which is cultivated with the ameliorant can expand its conventional functions as a seasoning to different purposes such as medicines and growth regulator. The purpose of this study is to determine the type and dose of ameliorant which are suitable in improving the productivity of a few varieties of onion (Allium ascalonicum L.). This research was conducted in Labuhan Deli District in the province of Sumatera Utara, Socfindo Laboratory and Final Disposal from February – June 2017. The experiment was carried out using a Randomized Block Design with 2 factors and 3 replications. The first factor is varieties consisting of 3 varieties (Bima Brebes, Super Philip, and Medan). the second factor are Ameliorant consisting of 5 dose levels (Control (without ameliorant), Biochar 0,4 kg/m2, biochar 0,8 kg/m2, Urban waste compost 1 kg and Urban waste compost 2 kg/m2). Parameters observed ie plant height (cm), Number of tillers per hill (tillers), and fresh weight production per m2. The results showed that varieties factor gave significantly of difference to the urban waste compost aplication 1 kg can increase the production of super Philip varieties onion with total fresh weight production 1203,57 g.Keywords: Onion, Varieties, Ameliorant
Co-Authors Agus Pernando Simanjuntak Amaluddin Syahputra Ameilia Zuliyanti Siregar Andre Leo H Saragih Andreas Hutasoit Anna Eka ardy rahmadhani Arrasyid, Nurfida Khairina ASMARLAILI SAHAR Aznawi, Andi Aznan Chairani Hanum Chairani Hanum Christy, Julieta Dede Suhendra Dedy S Silaban Deliana Delvian Delvian Denny Satria, Denny Desta Wirnas DIAH PUSPITA ANWAR Dini Rizkita Pulungan Dohude, Gostry Aldica Dunan Naibaho E. Harso Kardhinata Edison Purba Edison Purba Elfiati, Deni Elfiati Ella Yula Ella Wati Simamora Emmy Harso Kardhinata Emmy Harso Kardhinata Emmy Harso Kardhinata etna adriana silaban etti sartina siregar Eva Sartini Bayu Evan Satria Saragih Evi Julianita Harahap Frans R Sipayung Gali Rakasiwi gredo oktavianus mario purba Guswinda N Sitanggang hani maura tiarli Harahap, Nurul Izzatunna Jhirah Hasanuddin Hasanuddin Heri Arifianto Hot Setiado Irda Safni Irfansyah Irfan irvan maulana amry Irwan Bonar Sibarani Isman Nuriadi Iwan RISNASARI Josua Simanjuntak Jumilawaty, Erni Jumilawaty Kiking Ritarwan Koko Tampubolon Lahay, Ratna Rosanty Larosa Harahap Lidya Sundari Lollie Agustina P Putri Lollie Agustina P. Putri Lollie Agustina P. Putri lubis, khairunnisa Luta, Devi Andriani Luthfi A. M. Siregar Luthfi A.M Siregar Luthfi Aziz Mahmud Siregar Mariati Sinuraya Mbue Kata Bangun Meylin Kristina Saragih Mohammad Basyuni Mubaraq, Alfian Mubaraq Muhdi Muhdi Muhdi Muhdi Muhdi Muhdi Muhdi Mutia Rahmah Nauly, Meutia Nauly Nazriah pratiwi Nisma Khairani Harahap Nurul Ain Lubis Nurul Hidayah Sinaga Olivia Avriyanti Hanafiah Onrizal Onrizal Pohan, Riko Andika Putri, Maharani Syahnia Rahmi Ami Rangkuti, Ahmad Baiquni Revandy Iskandar Muda Damanik Ria Arta Junistia Ridwanti, Batubara Risma Damayanti Risnasari, Iwan Risnasari Rohani Ganie, Rohani Ganie Rosmayanti Rosmayanti Rosmayati Rosmayati Rosmayati Rosmayati Rosmayati Rosmayati Salman Alfarisi Simangunsong Samosir, Zurni Zahara Samsuri Sartika EC Siallagan Siti Rachma Aulia Damanik Sivaipram, Itchika Sivaipram Slamet, Bejo Slamet Sri Yunita Simanjuntak Sudirman Yahya Sudirman Yahya Sudirman Yahya - Susilowati, Arida Susilowati Syaflida, Rahmi T Sabrina T. Chairun Nisa T. Sabrina Trikoesoemaningtyas ungu, retno kencana Y Husni Yoke Blandina Larasati Sihombing