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All Journal HAYATI Journal of Biosciences Jurnal Silvikultur Tropika (JST) Jurnal Floratek Proceedings of Annual International Conference Syiah Kuala University - Life Sciences & Engineering Chapter BIOTROPIA - The Southeast Asian Journal of Tropical Biology AL KAUNIYAH BIOTIK: Jurnal Ilmiah Biologi Teknologi dan Kependidikan Journal of Aceh Physics Society Jurnal Penelitian Pendidikan IPA (JPPIPA) Prosiding Seminar Nasional Biologi, Teknologi dan Kependidikan (Biotik) Makara Journal of Science Jurnal Natural Journal of Patchouli and Essential Oil Products Jurnal Ilmiah Mahasiswa Pertanian Heca Journal of Applied Sciences Grimsa Journal of Science Engineering and Technology Biosaintifika: Journal of Biology & Biology Education Biosainsdik: Biologi Sains dan Kependidikan
Essy Harnelly
Biology Department, Faculty Of Mathematics And Natural Sciences, Syiah Kuala University
Agriculture, Biological Sciences & Forestry Astronomy Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Decision Sciences, Operations Research & Management Dentistry Earth & Planetary Sciences Economics, Econometrics & Finance Education Energy Engineering Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Library & Information Science Materials Science & Nanotechnology Medicine & Pharmacology Neuroscience Nursing Physics Public Health Social Sciences Veterinary Other

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Struktur dan Komposisi Vegetasi Pada Kawasan Lindung Desa Kekuyang Kecamatan Ketol Aceh Tengah Suryadi Suryadi; Ulfa hansri Ar-Rasyid; Essy Harnelly
Jurnal Ilmiah Mahasiswa Pertanian Vol 8, No 2 (2023): Mei 2023
Publisher : Fakultas Pertanian, Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17969/jimfp.v8i2.24449

Abstract

Abstrak Kawasan lindung adalah kawasan yang ditetapkan memiliki fungsi utama untuk melindungi ekosistem, mengatur tata air dan pengendalian erosi, namun pada kawasan lindung di Desa Kekuyang fungsi tersebut sudah mulai terganggu dikarenakan adanya perkebunan masyarakat di dalam kawasan lindung. Tujuan dari penelitian ini adalah untuk mengetahui struktur dan komposisi vegetasi pada kawasan lindung Desa Kekuyang, Kecamatan Ketol Aceh Tengah. Penelitian dilakukan pada tiga tipe hutan yaitu hutan sekunder tua, hutan sekunder dan agroforestri kopi. Penelitian ini telah dilakukan pada bulan agustus 2022 di kawasan lindung Desa Kekuyang, Kecamatan Ketol, Aceh Tengah dengan menggunakan metode systematic with random start dengan plot berjumlah 23 plot, setiap plot di bagi menjadi plot semai, pancang, tiang dan pohon. Dari hasil penelitian tentang struktur dan komposisi ditemukan bahwa struktur dan komposisi jenis vegetasi dihutan sekunder tua ditemukan 15 jenis, dan pada hutan sekunder ditemukan 19 jenis, dari kedua transek didapatkan 13 jenis tumbuhan penyusun yang sama yaitu jenis bayur, suren, nyatoh, jeluntung, kenari, gayam, jelantang gajah, meranti merah, ulin, medang sahang, luwingan, kareumbi dan mara. Sedangkan pada transek agroforestri kopi hanya ditemukan 10 jenis dan hanya dua jenis tumbuhan penyusun yang sama dengan hutan sekunder yaitu jenis aren dan kemiri.Analysis Of Vegetation Stucture and Composition in The Protected Area Of Kekuyang Village Ketol District Central AcehAbstract. Protected area is an area that is determined to have the main function of protecting ecosystems, regulating water management and erosion control, but in protected areas in Kekuyang village this function has begun to be disrupted due to the presence of community plantations in protected areas. The purpose of this research is to determine the structure and vegetation composition as well as knowing the diversity, evenness and richness of vegetation types. The research was conducted in three types of forest, namely old secondary forest, secondary forest and coffee agroforestry. This research was conducted in August 2022 in the protected area of Kekuyang Village, Ketol District, Central Aceh using the systematic method with random start with a plot of 23 plots, each plot was divided into plots of seedlings, saplings, poles and trees.From the results of research on structure and composition it was found that the structure and composition of vegetation species in old secondary forest found 15 species, and in secondary forest found 19 species, from both transects obtained 13 species of the same constituent plants namely bayur, suren, nyatoh, jeluntung, walnut, gayam, elephant nettles, red meranti, ironwood, medang sahang, luwingan, kareumbi and mara. Meanwhile, in the coffee agroforestry transect, only 10 species were found and only two species of plants that make up the same as the secondary forest, namely sugar palm and candlenut species.
POPULATION STRUCTURE OF DIPTEROCARPACEAE SPECIES IN KETAMBE RESEARCH STATION, GUNUNG LEUSER NATIONAL PARK, ACEH TENGGARA Rizki Amelia; Saida Rasnovi; Essy Harnelly
Jurnal Natural Volume 19, Number 1, February 2019
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24815/jn.v19i1.12869

Abstract

Ketambe Research Station is one of the oldest and most complete stations in the world which has abundant biodiversity. The Dipterocarpaceae is one of the family identified as growing in the forest area of Ketambe Research Station. Ecologically, Dipterocarpaceae has several limiting factors for its growth and distribution and the most determining factors are soil factors and climate. Dipterocarpaceae is also known as nest and source of food for orangutans, namely Shorea spp. Therefore, it is necessary to conduct research that aims to determine the population structure of Dipterocarpaceae species in Ketambe Research Station. The method used Quadrat Sampling Technique. Theplacement of the sampling plot was randomly carried out in 25 plots with an area of 1 ha. The result showed that there were three species of Dipterocarapaceae found in this station i.e., Parashorea lucida, Shorea johorensis and Hopea dryobalanoides. Based on the population structure pyramid, P. lucida will survive and develop in the future S. johorensis and H. dryobalanoides, on the other hand, are estimated to decrease or hardly survived in the future.
Comparison of two DNA extraction methods for dry Leaf Dipterocarpaceae ESSY HARNELLY; NITA TAUHIDA; ISKANDAR ZULKARNAIN SIREGAR
Jurnal Natural Volume 22 Number 3, October 2022
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24815/jn.v22i3.26588

Abstract

DNA extraction is the first step in obtaining high-concentration and high-purity DNA that can use in the subsequent steps. Because the leaf structure is challenging and contains several secondary metabolites that can affect DNA extraction results, DNA extraction on dry leaves of Dipterocarpaceae is considered problematic. This research aims to find a suitable method for extracting DNA from the preserved Dipterocarpaceae leaves. Preserved leaves of Dipterocarpaceae in silica gel will dry up, making them tough to destroy. Perform DNA extraction is generally using CTAB buffers or extraction kits. According to the results of this study, extraction with modified CTAB buffer result in DNA with higher concentration and purity values than DNA extracted with the kit. DNA isolated from the modified CTAB can be used as a template in the PCR process to amplify the matK gene.
Diversity and population structure of Dipterocarpaceae species in Weh Island Nature Tourism Park, Indonesia RIDHA UL FAHMI; SAIDA RASNOVI; ESSY HARNELLY
Jurnal Natural Volume 21 Number 2, June 2021
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24815/jn.v21i2.20300

Abstract

The Dipterocarpaceae family is one of the identified plant groups with high conservation value that exist in Weh Island Nature Tourism Park. However, the data availability on the species diversity and population structure of Dipterocarpaceae in Weh island in particular and Aceh in general is still very limited. Thus, it is necessary to conduct studies on species diversity and population structure of Dipterocarpaceae from Weh Island. This study aims to obtain data on species abundance and diversity, species composition of each stratum, population structure, and stand structure of Dipterocarpaceae in Weh Island Nature Tourism Park. This research utilized transect method with randomly selected plots according to topographic conditions. We found two Dipterocarpaceae species: Shorea sumatrana (Slooten) Desch and Dipterocarpus hasseltii Blume. Shorea sumatrana (Slooten) Desch has the greatest abundance of all types and strata. The diversity of Dipterocarpaceae species in Weh Island Nature Tourism Park is categorized as low because the diversity index value is in the range of H'1. This means that the presence of seedling and sapling strata from each species has a major effect on the survival of the Dipterocarpaceae family in the future. The stand structure of the Dipterocarpaceae family in normal conditions is indicated by an inverted “J” shaped diagram.
MOLECULAR IDENTIFICATION OF SHOREA JOHORENSIS IN KETAMBE RESEARCH STATION, GUNUNG LEUSER NATIONAL PARK Nir Fathiya; Essy Harnelly; Zairin Thomy; Iqbar Iqbar
Jurnal Natural Volume 18, Number 2, June 2018
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24815/jn.v18i2.10123

Abstract

Shorea johorensis is one of the red meranti plants in Ketambe Research Station, Gunung Leuser National Park, Aceh Tenggara. Currently, Shorea johorensis also is well known as a major source of valuable commercial timber. This research aimed to analyze the phylogenetic of Shorea johorensis based on chloroplast and nuclear DNA in Ketambe Research Station so that it can be known the relationship of Shorea johorensis with other species Dipterocarpaceae in GenBank database. The research was conducted from July 2015 to August 2016 in Ketambe Research Station and Forestry and Forest Genetics Laboratory of Molecular, Bogor Agricultural University. The method used quadrat sampling technique with purposive sampling and experimental laboratory that consisted of DNA extraction, PCR, electrophoresis, and sequencing. The data analysis was done using BioEdit, MEGA6, and BLAST. The results showed that the phylogenetic tree of Shorea johorensis based on the rbcL and matK showed that Shorea johorensis was closely related with some species Hopea; but the phylogenetic tree based on psbA-trnH, 5.8S rRNA, ITS2, and 28S rRNA showed that Shorea johorensis was closely related with Shorea robusta.Keywords: Chloroplast dna, Ketambe Research Station,Nuclear DNA, Shorea johorensis
Anatomical changes of Ipomoea reptans due to mercury uptake and accumulation in contaminant soil NITA TAUHIDA; ESSY HARNELLY; MUHAMMAD NASIR; MUHAMMAD BAHI
Jurnal Natural Volume 22 Number 1, February 2022
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24815/jn.v22i1.23198

Abstract

Heavy metal contaminants like mercury is a serious problem to human, animals, and some plants’ health. Phytoremediation is an alternative technique, which can remediate the contaminants from soil using a hyperaccumulator plant. The goal of this research was to study anatomical changes of plant main organs (roots, stems, and leaves) of  Ipomoea reptans which are assumed as a hyperaccumulator plant that grows in mercury contaminant. The mercury concentration of the growth medium was 0, 61.871, 92.258, and 107.046 ppb. Ipomoea reptans were harvested after 27 days. The anatomical changes of the plant’s main organs were observed by preparing the cross-section of roots, stems, and leaves of I. reptans. The result showed that mercury treatment has caused anatomical damage at the xylem vessel of the root, and decreased bulliform cell size. The anatomical damage was found only in the root of I. reptans. The mercury concentration in media decrease to 0 ppb (P0), 50,420 ppb (P1), 58,583 (P2), and 96,120 (P3).
PHYLOGENETIC RELATIONSHIPS WITHIN FAMILIES MYRTACEAE IN TRIPA PEAT SWAMP FOREST USING INTERNAL TRANSCRIBED SPACER (ITS) Ardhana Yulisma; Zairin Thomy; Essy Harnelly
Jurnal Natural Volume 18, Number 2, June 2018
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24815/jn.v18i2.10105

Abstract

The Tripa peat swamp forest is located on the west coast of Aceh province and falls administratively under the two districts of Nagan Raya and Aceh Barat Daya. Tripa peat swamp forest has a high diversity of plants. The previous research discovered that Myrtaceae is one of the dominant families in the forest. This study aimed to predict phylogenetic relationships among trees species within families Myrtaceae that live in the Tripa peat swamp forest using Internal Transcribed Spacer (ITS). The research was conducted at Laboratory of Forest Genetics and Molecular Forestry, Faculty of Forestry, IPB from June 2015 to December 2016. The methods were conducted with an experimental laboratory of molecular identification consisting of DNA extraction, amplification, and sequencing. Data was analyze using BioEdit, Clustal X, Mega 6, and BLAST. The morphology identification reported that there were four trees species within families Myrtaceae, i.e., Syzygium sp 1, Syzygium garciniifolium, Syzygium leptostemon and Tristaniopsis whiteana. Reconstruction of phylogenetic tree using the Neighbor-Joining (NJ) method 1000x showed that the ITS region was successfully to predict phylogenetic relationships within genus Syzygium and Tristaniopsis.Keywords: tripa peat swamp forest, tree species, phylogenetic, myrtaceae, internal transcribed spacer
THE EFFECT OF SOME OF COCONUT WATER CONCENTRATION IN ARTIFICIAL MEDIA TO CHRYSANTHEMUM GROWTH (Dendranthema grandiflora) BY IN VITRO Nurur Rahmy; Zairin Thomy; Yunita Yunita; Essy Harnelly
Jurnal Natural Volume 19 Number 2, June 2019
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24815/jn.v19i2.11541

Abstract

Artificial medium for in vitro culture is an alternative media to subtitute Murashige and Skoog (MS). Growmore added with organic substances as coconut water can be used as artificial medium. Coconut water can affect plant growth, so the research is to investigate the influence of coconut water in different concentration to chrysanth’s (Dendranthema grandiflora) growth should be done. The research was conducted in Laboratory of Molecular and Cell Biology, Department of Biology, Faculty of Mathematics and Natural Sciences, Syiah Kuala University Darussalam, Banda Aceh, from April until July 2013. The research was designed by using Randomized Block Design with sub sampling. Media act as group, while coconut water concentration as treatment. Observation was started from growing time up to a month after planting. The result showed that there was no significant different between using MS and artificial medium with different coconut water concentration to initiate shootlet.
Exploration of potential wild medicinal mushrooms from Pocut Meurah Intan forest park, Aceh, Indonesia HENDRIX INDRA KUSUMA; ESSY HARNELLY; ZAIRIN THOMY; MUHAMMAD ADRIYAN FITRA; SAMINGAN SAMINGAN
Jurnal Natural Volume 20 Number 3, October 2020
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24815/jn.v20i3.16916

Abstract

Mushroom is one of living things with abundant diversity and has various beneficial potentials; one of them is as medicine. The use of mushrooms as medicine has long been known primarily as traditionally made herbs and the knowledge has been passed down for generations. The Pocut Meurah Intan Forest Park as a conservation forest, stores a variety of mushrooms in it. Data about the type of mushroom that has medicinal potential existed in the forest park has never been published so it is important to do so. This study aims to record the types of mushrooms that have benefits as medicines. The method used is an exploratory survey through a trail or tracking path then identified as a medicinal mushroom base on literature studies. The results of the study found 15 species of mushrooms that have potential as medicine, namely Cookeina tricholoma, Sarcoscypha coccinea, Tremella fuciformis, Auricularia auricula-judae, Flammulina velutipes, Schizophyllum commune, Scleroderma citrinum, Boletus edulis, Trametes versicolor, Lentinus tigrinus, Lentinus squarosulus, Ganoderma applanatum, Ganoderma resinaceum, Thelephora ganbajun and Lycoperdon echinatum. This shows that Pocut Meurah Intan Forest Park has great resources and potential that can be utilized for the benefit of the surrounding community, and it needs to be preserved so that diversity is maintained and can be used by future generations.
Pro-Apoptosis Activity of Pogostemon cablin Benth. Against Nasopharyngeal Carcinoma through the BCL-2 Inhibition Signaling Pathway: A Computational Investigation Syahraini, Aigia; Harnelly, Essy; Hermanto, Feri Eko
Makara Journal of Science Vol. 27, No. 3
Publisher : UI Scholars Hub

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Resistance to chemotherapy and radiotherapy frequently emerges in the later stage of nasopharyngeal carcinoma (NPC) tumorigenesis. The decreased response of NPC to radiotherapy and chemotherapy occurs owing to the inhibition of cancer cell apoptosis by the B-cell lymphoma-2 (BCL-2) protein. Thus, inhibiting BCL-2 protein may become a powerful approach to eliminate NPC through apoptosis regulation. Meanwhile, Pogostemon cablin is reported to exhibit anticancer properties, but there are limited studies on its use for NPC treatment. The objective of this study is to investigate the potential bioactive compounds in P. cablin as anti-apoptosis BCL-2 protein inhibitors using in-silico approach. Natural compounds from P. cablin were retrieved from the KNApSAcK database and screened for inhibitory effects on BCL-2 protein via molecular docking coupled with molecular dynamics. It was found that apigenin, rhamnetin, and apigenin 7-(6″-p coumarylglucoside) showed potential inhibitory properties against BCL-2 protein based on binding affinity and interaction chemistry. The highest binding affinity was recorded for apigenin 7-(6″-p coumarylglucoside) at −9.9 kcal/mol, followed by rhamnetin and apigenin at −7.2 kcal/mol. These compounds are also bound to the inhibitory sites of BCL-2 and venetoclax, mainly by hydrophobic bonds and Van der Waals interactions. Nevertheless, molecular dynamics simulations revealed that apigenin 7-(6″-p-coumarylglucoside) had unstable conformation and binding to BCL-2. In summary, this study demonstrated that P. cablin has excellent potency as an alternative or complementary therapy against radiotherapy and chemotherapy resistance of NPC, mainly through rhamnetin and apigenin.
Co-Authors Adrian Damora Agus Sara Alim Misbullah Amirunnas , Amirunnas Annisa Ammalia Kiti Ardhana Yulisma Arif Habibal Umam Ariqah, Nada Cici Ariska A Dina Firmadiana Faradhila, Jihan Fauzi, Fazlin Mohd Fauziah . Fenty Ferayanti Fikrul Akhyar Gani, Fadli A. Habib, Ahasan Hairul Basri Hawati Hawati HENDRIX INDRA KUSUMA Hermanto, Feri Eko Idroes, Ghifari Maulana Iqbar Iqbar Irma Dewiyanti Irma Sari ISKANDAR ZULKARNAIN SIREGAR Isnaini, Nadia Itawarnemi, Hilmina Khairan Khairan Kusuma, Hendrix Lenni Fitri Lydia Septa Desiyana, Lydia Septa M. Satria Fitri Malahayati T. Hanafiah Martunis - Martunis Martunis Maysarah, Hilda Meutia Zahara Mira Humaira Misrahanum Misrahanum MUHAMMAD ADRIYAN FITRA MUHAMMAD BAHI Muhammad Irfan Muhammad Rusdi Muhammad Subianto Munira, Alya Mutia Zahara Nafissa, Naja Nanda Muhammad Razi Nazaruddin Nir Fathiya NITA TAUHIDA NITA TAUHIDA Nur Fadli Nurur Rahmy Oktaviana, Nurul Onny Ulfa Rahayu Prajaputra, Vicky Rahayu, Sri Riska Ramadhaniaty, Mutia Rauzana, Anita Razief Perucha Fauzie Afidh RIDHA UL FAHMI Rina Sriwati Rinaldi Idroes Rizki Amelia Rizki, Alia Rizky Amelia Rusita Fitri Saida Rasnovi SAIDA RASNOVI Saida Rasnovi Samingan Samingan Sari, Febia Saudah Saudah SITI MARYAM Siti-Azizah, Mohd Nor Sreeramanan Subramaniam Sri Jumiati, Sri Sri Wati Suryadi Suryadi Syaharani, Cut Puspita Salsabila Syahraini, Aigia Syaifullah Muhammad Teuku Rizky Noviandy Ulfa hansri Ar-Rasyid Wira Dharma Wira Dharma Yekki Yasmin Yudi Haditiar Yunita Yunita Yurinda Yurinda ZAINAL ABIDIN MUCHLISIN Zairin Thomy Zairin Thomy Zairin Thomy Zairin Thomy Zairin Thomy ZAIRIN THOMY Zairin Thomy Zairin Thomy Zairin Thomy Zakaria, Rahmad Zulfan Zulkarnain Zulkarnain Zulwanis, Zulwanis Zumaidar Zumaidar