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All Journal HAYATI Journal of Biosciences El-Hayah : Jurnal Biologi Jurnal Matematika dan Ilmu Pengetahuan Alam Journal of Tropical Biodiversity and Biotechnology Jurnal Biodjati BIOTROPIC The Journal of Tropical Biology Jurnal Layanan Masyarakat (Journal of Public Service) Jurnal Riset Biologi dan Aplikasinya Cakrawala Repositori Imwi Jurnal Pengabdian kepada Masyarakat Nusantara Narra J Berkala Penelitian Hayati Folia Medica Indonesiana
Alfiah Hayati
Department Of Biology, Faculty Of Science And Technology Universitas Airlangga
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Computer Science & IT Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Environmental Science Health Professions Immunology & microbiology Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Neuroscience Public Health Social Sciences Other

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Journal : Narra J

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Effect of pegagan (Centella asiatica) nanoparticle coated with chitosan on the cytokine profile of chronic diabetic mice Muchtaromah, Bayyinatul; Firdaus, Ana MK.; Ansori, Arif NM.; Duhita, Maharani R.; Minarno, Eko B.; Hayati, Alfiah; Ahmad, Mujahidin; Analisa, Izza
Narra J Vol. 4 No. 1 (2024): April 2024
Publisher : Narra Sains Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52225/narra.v4i1.697

Abstract

Diabetes is closely related to immune response problems when it occurs chronically. Pegagan (Centella asiatica) is a medicinal plant with active compounds. Madecassoside is beneficial in treating diabetes, and nanoparticle technology is expected to enhance the medicinal potential and availability of pegagan compounds. The aim of this study was to determine the effect of chitosan-coated pegagan nanoparticles on the cytokine profile of chronic diabetic mice, which included CD4+TNF-α+, CD8+TNF-α+, CD4+IFN-γ+, CD8+IFN-γ+ and IL-6+. An experimental study with a randomized complete block design (CRD) consisting of six treatments with seven replicates was conducted. The groups were: healthy mice as negative control; diabetic mice treated with distilled water as positive control and diabetic mice treated with nanoparticle coated with chitosan (NPC) 20 mg/kg, 30 mg/kg, 40 mg/kg, and metformin 130 mg/kgBW. The data were tested using one-way analysis of variance (ANOVA) with a significance level of 5% and continued with the Duncan’s multiple range test. The results showed that pegagan NPC could significantly reduce the relative number of CD4+TNF-α+, CD8+TNF-α+, CD4+IFN-γ+ and CD8+IFN-γ+ and IL-6 in the dose of 20 mg/kg, 30 mg/kg and 40 mg/kg (p<0.05). The treatment dose of 20 mg/kg reduced CD4+TNF-α+, CD8+TNF-α+, CD4+IFN-γ+, CD8+IFN-γ+ to the levels of healthy mice and a dose of 30 mg/kg could reduce IL-6 as in healthy mice. These findings suggest that chitosan-coated pegagan nanoparticles are a promising therapy for diabetes, as they have the potential to modulate the immune response associated with chronic diabetes.
Plant-based synthesis of gold and silver nanoparticles using Artocarpus heterophyllus aqueous leaf extract and its anticancer activities Dewi, Firli RP.; Rohmatika, Aulia U.; Jamil, Arniza KM.; Demircan, Turan; Idris, Muhammad F.; Litazkiyyah, Litazkiyyah; Fahmi, Muhammad; Rosyidah, A'liyatur; Hayati, Alfiah; Sugiharto, Sugiharto
Narra J Vol. 5 No. 2 (2025): August 2025
Publisher : Narra Sains Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52225/narra.v5i2.1770

Abstract

Green synthesis of nanoparticles has garnered significant attention for its sustainable and environmentally friendly approach. Despite extensive research on Artocarpus heterophyllus-derived nanoparticles using seeds, fruits, and rind, the therapeutic potential of its leaf extract remains largely unexplored, particularly in MCF-7 breast cancer cells. The aim of this study was to investigate the potential of aqueous leaf extract from A. heterophyllus as a reducing and capping agent to synthesize silver nanoparticles (AgNPs) and gold nanoparticles (AuNPs), as well as to evaluate their anticancer efficacy. The nanoparticles were characterized using ultraviolet-visible spectroscopy, transmission electron microscopy, Fourier-transform infrared spectroscopy, X-ray diffraction, and particle size analysis to confirm the formation. To evaluate anticancer potential, key oncogenes associated with cancer proliferation and survival were analyzed, including c-Myc, cyclin D1, human epidermal growth factor receptor-2 (HER-2), microRNA-622 (miR-622), and cyclooxygenase-2 (COX-2). The present study demonstrated that AgNPs and AuNPs synthesized from A. heterophyllus extract had distinct sizes and shapes, with AgNPs averaging approximately 12.75 nm and exhibiting a spherical morphology, while AuNPs averaged 109.26 nm and had a pentagonal shape. Furthermore, AuNPs had no anticancer activity. In contrast, AgNPs showed potent anticancer effects, with inhibitory concentration (IC50) values of 124.626 and 54.981 µg/mL at 48 and 72 hours, respectively. The AgNPs treatment increased the proportion of cells in G2/M phase, indicating the induction of mitotic catastrophe leading to cell death. AgNPs downregulated the expression of several oncogenes associated with cancer cell proliferation and survival (cyclin D1, COX-2, HER-2, and miR622), but did not significantly reduce c-Myc expression. In conclusion, AgNPs derived from A. heterophyllus leaf extract have significant potential as a novel therapeutic agent in cancer treatment while preserving its biocompatibility, emphasizing the promise of sustainable and cost-effective synthesis of plant-based nanoparticles.
Co-Authors Adelah Putri Milenia Sari Affandi, Moch Agoes Soegianto Agus Supriyanto Agustina, Widayati Almando Geraldi Amin, M. Hilman F. Analisa, Izza Analisa, Nur Izzah Ansori, Arif NM. Antien Rekyan Seta Aucky Hinting Aunurohim Aurira Thrisna Dwi Aprianti Azizaturrohmah Bayyinatul Muchtaromah Berlian Rustantina Binti Mar`atus Solikha Demircan, Turan Dewi, Firli RP. Dewi, Putri Alicia Duhita, Maharani R. Dwi Winarni Ely Nuril Fajriyah Erlyn Nurul Fauziah Erna Agustina Erna Agustina Fajriyah, Ely Nuril Farah Annisa Nurbani fatimah Fatimah Fatma Aulia Nadiyah Fatmi Indah Hati Fauziah, Erlyn Nurul Firdaus, Ana MK. Firli Rahmah Primula Dewi Gebby Agnessya Esa Oktavia Habibi, Soraya Hana Widyana Hanik Faizah I.B. Rai Pidada Idris, Muhammad F. Iman Dary Supriyadi Putra Inesavira Rindaputri Inne Ninda Resita Jamil, Arniza KM. Junairiah Junairiah Khaleyla, Firas Khusnita Giarti Ladiya Fitri Ma’rufah Laili Musyarofah Lestari, Lita I.D. Litazkiyyah, Litazkiyyah Manikya Pramudya Maya Safitri Maya Safitri Minarno, Eko B. Mochammad Affandi Muhamad Fadhil Mirza Muhamad Hilman Fu'adil Muhamad Maulana Abdizen MUHAMMAD FAHMI Muhammad Hilman Fu'adil Amin Mujahidin Ahmad Nabilla, Nithasya Nashrulloh, Allam Thoriq Nazil Dwi Rahayuningtyas Ni Wayan Sukma Antari Nimatuzahroh, Nimatuzahroh Nina Puspita Ninik Fadhillah Nita Yuliarini Nur Izzah Analisa Nurbani, Farah Annisa Nureka Tiantono Nuris Auliya Nurul Mahmudati Prilya Dewi Fitriasari Princessa Nandita Febrionny Resita, Inne Ninda Risanda Ulinnuha Rohmatika, Aulia U. Rosmanida Rosyidah, A'liyatur S. Sugiharto Salamun Salamun Salsabila, Syarifah Septiani, Adiibtia Setiyowati, Putri Ayu Ika Soesanto Mangkoewidjojo Soraya Habibi Sri Puji Astuti Wahyuningsih Sri Puji Astuti Wahyuningsih, Sri Puji Astuti Sri Sukmaningrum Sugiharto Suhestri Suryaningsih Sukarti Moeljopawiro Tri Nurhariyati Trisnadi Widyaleksono Catur Putranto Umi Nur Alfiah Ummi Zubaidah Virid Gibson, Virid Vuanghao Lim Vuanghao, Lim Widi Pangestu Wilujeng Widjiati Widjiati Widjiati Widjiati, Widjiati Yuli Winarsih