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All Journal International Journal of Public Health Science (IJPHS) Jurnal Gizi ASPIRATOR Disease Prevention and Public Health Journal Public Health of Indonesia THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST Jurnal Kedokteran dan Kesehatan Meditory : The Journal of Medical Laboratory JBN (Jurnal Bedah Nasional) Journal of Epidemiology and Public Health Indonesian Journal of Medicine Journal of Maternal and Child Health Afiasi : Jurnal Kesehatan Masyarakat Jurnal Pengabdian Masyarakat IPTEKS Abdimasku : Jurnal Pengabdian Masyarakat Indonesian Journal of Global Health research Jurnal Penelitian Sekolah Tinggi Ilmu Kesehatan Nahdlatul Ulama Tuban Jurnal Cakrawala Promkes Epidemiology and Society Health Review (ESHR) Jurnal Pengabdian Kesehatan Komunitas (Journal Of Community Health Service) JURNAL INOVASI DAN PENGABDIAN MASYARAKAT INDONESIA Ahmad Dahlan Medical Journal International Journal of Healthcare Research Jurnal Penelitian ASPIRATOR - Journal of Vector-Borne Diseases Studies Kesmas: Jurnal Kesehatan Masyarakat Nasional (National Public Health Journal)
Fardhiasih Dwi Astuti
Faculty Of Public Health, Universitas Ahmad Dahlan, Yogyakarta
Religion Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Computer Science & IT Dentistry Economics, Econometrics & Finance Education Environmental Science Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Languange, Linguistic, Communication & Media Medicine & Pharmacology Neuroscience Nursing Public Health Social Sciences Veterinary

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Journal : THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST

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CAMPURAN INFUSA SINGKONG (Manihot esculenta), KACANG KEDELAI (Glycine max (L.) Merrill) DAN EKSTRAK RAGI SEBAGAI MEDIA ALTERNATIF PERTUMBUHAN BAKTERI Staphylococcus aureus Martha Atik Martsiningsih; Fardhiasih Dwi Astuti; Menik Kasiyati; Suyana Suyana; Ullya Rahmawati; Muji Rahayu; Ajeng Puspa Aeni
THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST Vol 7 No 1 (2024): The Journal of Muhammadiyah Medical Laboratory Technologist
Publisher : Universitas Muhammadiyah Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30651/jmlt.v7i1.19863

Abstract

The composition of nutrients needed by bacteria varies greatly such as peptone, meat extract, and agar. Nutrient Agar is one of the media used as a bacterial culture that has a composition such as protein and carbohydrates in the form of meat extract and peptone that suits the needs of most bacteria. The carbohydrate content in cassava, protein in soybeans, and nitrogen sources in yeast extract can be combined into alternative media used as bacterial growth media to determine whether the alternative media combination of cassava carbohydrate source infuse, soybean infuse, and yeast extract can be used to grow Staphylococcus aureus bacteria. This research is a pure experimental study with Post Test-Only Control Design research design. The mean growth in the number of colonies of Staphylococcus aureus bacteria on alternative media was 78,25 x  CFU/ml and NA media was 94.19 x   CFU/ml with a mean difference of 15.94 x  CFU/ml or 16.92%. The mean growth diameter of the number of colonies of Staphylococcus aureus bacteria on alternative media was 2.63 x  CFU/ml and NA media was 2.66 x   CFU/ml with a mean difference of 0.03 x  CFU/ml or 1.12%. The percentage of effectiveness of Staphylococcus aureus bacterial growth is 83.07% or moderately effective and the percentage of effectiveness of Staphylococcus aureus bacterial proliferation is 98.87% or effective. Alternative media mixture of cassava infuse (Manihot esculenta crantz), soybean (Glycine max (L.) Merrill) and yeast extract can be used to grow Staphylococcus aureus bacteria.
CAMPURAN INFUSA SINGKONG (Manihot esculenta), KACANG KEDELAI (Glycine max (L.) Merrill) DAN EKSTRAK RAGI SEBAGAI MEDIA ALTERNATIF PERTUMBUHAN BAKTERI Staphylococcus aureus Martsiningsih, Martha Atik; Astuti, Fardhiasih Dwi; Kasiyati, Menik; Suyana, Suyana; Rahmawati, Ullya; Rahayu, Muji; Aeni, Ajeng Puspa
THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST Vol 7 No 1 (2024): The Journal of Muhammadiyah Medical Laboratory Technologist
Publisher : Universitas Muhammadiyah Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30651/jmlt.v7i1.19863

Abstract

The composition of nutrients needed by bacteria varies greatly such as peptone, meat extract, and agar. Nutrient Agar is one of the media used as a bacterial culture that has a composition such as protein and carbohydrates in the form of meat extract and peptone that suits the needs of most bacteria. The carbohydrate content in cassava, protein in soybeans, and nitrogen sources in yeast extract can be combined into alternative media used as bacterial growth media to determine whether the alternative media combination of cassava carbohydrate source infuse, soybean infuse, and yeast extract can be used to grow Staphylococcus aureus bacteria. This research is a pure experimental study with Post Test-Only Control Design research design. The mean growth in the number of colonies of Staphylococcus aureus bacteria on alternative media was 78,25 x  CFU/ml and NA media was 94.19 x   CFU/ml with a mean difference of 15.94 x  CFU/ml or 16.92%. The mean growth diameter of the number of colonies of Staphylococcus aureus bacteria on alternative media was 2.63 x  CFU/ml and NA media was 2.66 x   CFU/ml with a mean difference of 0.03 x  CFU/ml or 1.12%. The percentage of effectiveness of Staphylococcus aureus bacterial growth is 83.07% or moderately effective and the percentage of effectiveness of Staphylococcus aureus bacterial proliferation is 98.87% or effective. Alternative media mixture of cassava infuse (Manihot esculenta crantz), soybean (Glycine max (L.) Merrill) and yeast extract can be used to grow Staphylococcus aureus bacteria.
Co-Authors Achmad Arman Subijanto, Achmad Arman Aeni, Ajeng Puspa Agil Dwi Prasetya Ahmad Fadly Aini, Fitha NurIzza Qurratul Ajeng Puspa Aeni Annisa Sani Arif Sujagat Arifiani, Ezra Sabrina Arlina Azka Artanti, Galuh Asep Rustiawan Aulia Putri Nugraheni Desi Nurfita DEWI FITRIANI Dwi Utami Dyah Suryani Eka Irma Saputri Elia, Tasya Erni Gustiina Eti Poncorini Pamungkasari Eva Muslimawati Saputri Fatimah, Bunga Fikri, Muhammad Ali Hastuti, Siti Kunia Widi Hastuti, Siti Kurnia Widi Hasuti, Siti Kurnia Widi Heriyanto Putri, Rona Hafida Heriyanto, M. Junaidy Heriyanto, Moch Junaidy Ike Lukita Padmawati Indriyani, Mila Melinda Kasiyati, Menik Khairani Fauziah Liena Sofiana Maretalinia, Maretalinia Marsiana Wibowo Martha Atik Martsiningsih Martini, Titim Martsiningsih, Marta Atik Martsiningsih, Martha Atik Menik Kasiyati Muamar Afdhal Mahendra Muji Rahayu Muji Rahayu Musyafaah, Novita Siti Nisrina Zahira Haqi Nurul Qamariyah Padmawati, Ike Lukita Prasetya, Agil Dwi Pratiwi, Aulia Rahmadina Prisilla Maya Heni Pratiwi Puteri, Amelia Nurhaliza Putri Arum Sholekhati Putri, Rachma Greta Perdana Qurry Amanda Qurry Amanda Izhati Ramadhani Wahyuningtyas Rokhmayanti , Rokhmayanti Rokhmayanti Rokhmayanti Rokhmayanti, Rokhmayanti Rosyidah Rosyidah, Rosyidah Rulianawati, Viandika Sani, Annisa Saputri, Eva Muslimawati Selly Aprianda Syah Putri Septian Emma Dwi Jatmika Sistiyono Sistiyono, Sistiyono Siti Kurnia Widi Hastuti Suci Musvita Ayu Sufiati Bintanah Sujagat, Arif Sukamto, Ali Sukirto, Novi Wijayanti Sukmatama, Pradisa Ryan Sulistyawati Sulistyawati Sulistyowati, Taurina Fitriya Sulistyowati, Taurina Fitriya Sunarti Suyana Suyana Suyana Suyana, Suyana Suyitno Suyitno Taurina Fitriya Sulistyowati Titim Martini Titim Martini Tri Ratnaningsih Trisasri, Ruri Ullya Rahmawati ULLYA RAHMAWATI Utami, Budi Barata Kusuma Vitri Widyaningsih Wahyu Widyaningsih Wardani, Siska Wati, Erlin Susilo Waya Cahmawati Wibowo, Rena Anjeli Widea Rossi Desvita Wulandari, Ira Pebri Yulianto, Aris Yuniar Wardani