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Thomas Mata Hine
Fakultas Peternakan, Kelautan Dan Perikanan, Universitas Nusa Cendana
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Journal : Jurnal Nukleus Peternakan

 1   2 
SUPEROVULASI PADA INDUK SAPI BALI (SUPEROVULATION ON BALI COWS) Burhanuddin *; W. Marlene Nalley; Thomas Mata Hine; Heri Armadianto
JURNAL NUKLEUS PETERNAKAN Vol 1 No 1 (2014): Juni 2014
Publisher : Universitas Nusa Cendana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35508/nukleus.v1i1.696

Abstract

Pengaruh superovulasi pada korpus luteum (CL) sapi bali telah diteliti. Ten sapi bali tidak bunting berumur antara lima sampai tujuh tahun dalam kondisi baik, partus normal dan siklus estrus yang teratur digunakan dalam penelitian ini. Injeksi seri Prostaglandin (PGF2ᾳ) diikuti oleh suntikan gonadotropin eksogen yang diberikan kepada sapi sebagai perlakuan. Jumlah korpus luteum dievaluasi dan dikelompokkan menjadi tiga kategori tinggi (> 7), menengah (3-6) dan rendah (0-2). Kegiatan ovarium ditentukan dengan palpasi rektal terutama tentang jumlah korpus luteum setelah program superovulasi. Hasil penelitian menunjukkan bahwa 50% dari sapi memiliki rata-rata jumlah 4.00 CL (kategori sedang), 20% dengan rata-rata 7,50 CL (kategori tinggi), dan 30% adalah kategori rendah dengan rata-rata jumlah 1,7 CL.
PENGARUH SUPLEMENTASI TEPUNG BIJI ASAM TERFERMENTASI Saccharomyces cerevisiae DALAM RANSUM BASAL INDUK TERHADAP LITTER SIZE, BERAT LAHIR DAN PERSENTASE LAHIR HIDUP ANAK BABI PERANAKAN VDL Stef Amiron Ratu; Thomas Mata Hine; Johanis Ly
JURNAL NUKLEUS PETERNAKAN Vol 2 No 2 (2015): Desember 2015
Publisher : Universitas Nusa Cendana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35508/nukleus.v2i2.766

Abstract

The study purpose was to find out the best level of fermented tamarind seeds meal supplementation in pregnant sow’s basal diet performing the highest litter size, birth weight and born life percentage of newborn cross bred VDL piglets. There were 12 pregnant sows of 1 – 2 years of age with body weight average was 153.80Kg (CV= 12.22%) were used in the study. Block design 4 X 3 were administrated in the study. The 4 following diets were R0: basal diet (pregnant sow diet 15 CPl); R1: R0 + 5% fermented tamarind seeds meal, R2: R0 + 7,5% fermented tamarind seeds meal; and R3: R0 + 10% fermented tamarind seeds meal. The variables evaluated were birth weight and born life percentage. Analysis of variance (Anova) and Duncan’s multiple range test were applied in data analysis. Statistical results showed that effect of Saccharomyces cerivisiae fermented tamarind seeds meal was highly significant (P<0.01) on born life percentage, but not significant (p>0.05) on either litter size or birth weight of the new born piglets. The conclusion drawn in that supplementation of Saccharomyces cerivisiae fermented tamarind seeds meal 5 – 10% improved litter size, birth weight and born life percentage of the new born piglets ABSTRAK Penelitian ini bertujuan untuk mendapatkan level suplementasi terbaik tepung biji asam terfermentasi Saccharomyces cerivisiae dalam ransum basal induk bunting yang menghasilkan litter size, berat lahir dan persentase lahir hidup anak babi peranakan VDL terbaik. Penelitian ini menggunakan 12 ekor ternak babi betina berumur 1-2 tahun dengan rata – rata bobot badan awal 153,80 kg (KV=12,22%). Rancangan percobaan yang digunakan adalah Rancangan Acak Kelompok (RAK) terdiri dari 4 perlakuan dan 3 ulangan. Ransum perlakuan adalah R0: pakan basal (pakan induk 15 PK, tanpa tepung biji asam) R1: pakan basal + 5% tepung biji asam fermentasi, R2: pakan basal + 7,5% tepung biji asam fermentasi. R3: pakan basal + 10% tepung biji asam fermentasi. Variabel yang diuji adalah: litter size, berat lahir dan persentase lahir hidup anak. Prosedur analisis ragam dan uji jarak berganda Duncan digunakan dalam analisis data yang diperoleh. Hasil penelitian menunjukkan bahwa perlakuan suplementasi tepung biji asam terfermentasi Saccharomyces cerivisiae dalam ransum basal berpengaruh sangat nyata (P<0,01) terhadap persentase kelahiran hidup, tetapi berpengaruh tidak nyata (P>0.05) terhadap litter size dan bobot lahir anak. Kesimpulan dari penelitian ini adalah suplementasi tepung biji asam terfermentasi Saccharomyces cerevisiae 5-10% dalam ransum basal meningkatkan litter size, bobot lahir dan meningkatkan persentase lahir hidup anak.
TAMPILAN ESTRUS DAN TINGKAT KEBERHASILAN INSEMINASI BUATAN KAMBING KACANG YANG DIINDUKSI MENGGUNAKAN PROSTAGLANDIN F2α (ESTRONTM BIOVETA) DENGAN DOSIS YANG BERBEDA Soleman Manaze Weiri Sinda; Thomas Mata Hine; Wilmientje Marlene Nalley
JURNAL NUKLEUS PETERNAKAN Vol 4 No 2 (2017): Desember 2017
Publisher : Universitas Nusa Cendana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35508/nukleus.v4i2.826

Abstract

The aim of this study was to evaluate the effect of different levels of PGF2α (Estron ™ bioveta) induction on estrus performance and artificial insemination of kacang does. Sixteen parous kacang does (3-4 years) were used in the study. The aniamls were housed in group following a block randomized design of 4 treatments with 4 blocks. The 4 treatments offered were: injection with physiological NaCl (P0); 0.25 mL PGF2α (P1); 0.50 mL PGF2α (P2); and 0.75 mL PGF2α (P3). Variable measured were: estrus intensity, estrus percentage, estrus duration, service per conception, and conception rate. All data collected were descriptively analyzed. Kacang does injected with PGF2α 0.5-0.75 mL resulted in higher estrus (100%) for P2 and P3, respectively than those injected with 0, 25 mL (P1). The higher estrus intensity was recorded for does had treatment P1 and P2 (score 3) than those treated with P0 and P1 (score 1-2). Goats injected P2 and P3 had longer estrus duration (38.75 - 45.50 hours) than P1 and P0 (32.67- 37.33 hours). Furthermore, the higher conception rate were recorded for P1 (66.67% S/C 1.33), followed by P0 (33.33% S/C 1.67), P2 (0.75 S/C 1.75), and P3 (25% S/C 1.75), respectively. Therefore, it can be concluded that does injected with 0.25 mL PGF2α (Estron ™, Bioveta) had highest conception rate. ABSTRAK Penelitian ini bertujuan untuk mengetahui pengaruh induksi PGF2α dengan berbagai dosis yang berbeda terhadap tampilan estrus dan tingkat keberhasilan inseminasi buatan (IB) kambing kacang. Penelitian ini menggunakan kambing kacang betina sebanyak 16 ekor dengan umur 3-4 tahun. Ternak dikandangkan secara koloni mengikuti pola rancangan acak kelompok (RAK) dengan 4 perlakuan dan 4 ulangan. Perlakuan yang dicobakan adalah injeksi menggunakan NaCl fisiologis (P0), 0,25 mL PGF2α (P1), 0,50 mL PGF2α (P2), dan 0,75 mL PGF2α (P3). Parameter yang diukur adalah intensitas estrus, persentase estrus, lama estrus, service per conception, dan persentase kebuntingan. Hasil penelitian menunjukkan bahwa injeksi PGF2α sebanyak 0,5 mL (P2) dan 0,75 mL (P3) pada kedua perlakuan menghasilkan persentase estrus 100%, lebih tinggi daripada yang dinjeksi 0,25 mL (P1) hanya 75%. Tampilan intensitas estrus lebih tinggi pada perlakuan P1 dan P2 dengan skor 3, sedangkan P0 dan P1 hanya menunjukkan intensitas estrus dengan skor 1-2. Demikian juga dengan lama estrus P2 dan P3 mencapai 38,75 – 45,50 jam pada perlakuan, lebih lama daripada P1 dan P0 dengan lama estrus 32,67 – 37,33 jam. Angka kebuntingan tertinggi ditunjukkan pada perlakuan P1 66,67% dan 1,33 untuk nilai services per conception diikuti dengan P0 yaitu 33,33% dan 1,67; dan angka kebuntingan terendah terdapat pada perlakuan P2 dan P3 dan 0,75 yaitu 25% dan 1,75. Dari hasil penelitian ini dapat disimpulkan bahwa injeksi PGF2α ( Estron™, Bioveta ) sebanyak 0,25 mL menghasilkan angka kebuntingan tertinggi
EFEKTIVITAS BERBAGAI KONSENTRASI MINYAK ZAITUN EKSTRA VIRGIN (OLEUM OLIVAE) DALAM PENGENCER SITRAT KUNING TELUR (The Effectivity of Various Virgin Extra Oil Concentration (Oleum Olivae) in Citrate Egg-Yolk Diluent on the Quality of Duroc Liquid Semen) Yohanis Umbu Daku Waluwanja; Wilmientje Marlene Nalley; Thomas Mata Hine; Kirenius Uly
JURNAL NUKLEUS PETERNAKAN Vol 6 No 2 (2019): Desember
Publisher : Universitas Nusa Cendana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35508/nukleus.v6i2.2101

Abstract

The purpose of this study was to determine the efect of extra virgin olive oil on the quality of duroc pig spermatozoa during preservation. Semen was collected twice a week using the golve hand method of two 3 and 4 years old male duroc boar wiht healthy body and reproductive organs. Good quality semen was diluted with citrate-egg yolk (C-EY) supplemented with extra virgin olive oil (EVOO) at various consentration: 0. 6. 8. 10 and 12 %. Diluted semen is stored at 18-20°C and sperm quality was evaluated every 8 hours. The resulsts showed that the duroc spermatozoa in CEY diluents supplemented with EVOO 12% (P4) showed higher quality (P <0,05) compared to others treatment. with motility 41.50±2.24%. viability 47.53±1.59%. abnormalities 5.27±0.19% and survival 65.6±0.44 hours. The conclusion of this study is that 12% extra virgin olive oil in citric-yolk diluents is more effective in maintaining the quality of liquid sperm duroc pigs. ABSTRAK Tujuan dari penelitian ini adalah untuk menguji pengaruh penambahan minyak zaitun extra virgin terhadap kualitas spermatozoa babi duroc selama preservasi. Semen ditampung dua kali seminggu menggunakan metode glove hand dari dua ekor babi duroc jantan berumur 3 dan 4 tahun dengan kondisi tubuh dan organ reproduksi yang sehat. Semen yang berkualitas baik diencerkan dengan sitrat-kuning telur (S-KT) yang disuplementasi dengan minyak zaitun extra virgin (MZEV) pada konsentrasi: 0,6,8,10 dan 12%. Semen yang telah diencerkan disimpan pada suhu 18-20 ᶱ C dan kualitas spermatozoa dievaluasi setiap 8 jam. Hasil penelitian menunjukkan bahwa spermatozoa yang dipreservasi dalam SKT yang disuplementasi oleh (SKT-MZ12) mempunyai kualitas yang lebih tinggi (P<0,05) dibandingkan dengan keempat perlakuan lainnya. Kualitas spermatozoa yang dipreservasi dalam pengencer SKT+MZEV 12% dan disimpan selama 64 jam menghasilkan motilitas 41,50±2,24%, viabilitas 47,53±1,59%, abnormalitas 5,27±0,19% dan daya tahan hidup 65,6±0,44 jam. Kesimpulan dari penelitian ini adalah penambahan minyak zaitun extra virgin 12% dalam pengencer sitrat- kuning telur lebih efektif dalam mempertahankan kualitas sperma cair babi duroc.
PENGARUH PEMBERIAN EKSTRAK HIPOFISA DAN PROBIOTIK ABG-O PADA ANAK BABI PERSILANGAN TERHADAP PENINGKATAN BERAT BADAN SAPIH, UKURAN LINEAR TUBUH DAN METABOLIT DARAH (The effect of giving pituitary and probiotic extracts abg-o to piglets crosses.....) Mardemus Misa; Wilmientje M Nalley; Thomas Mata Hine; Twenfosel Dami Dato; Maritje A Hilakore; Mariana Nenobais; Aloysius Marawali
JURNAL NUKLEUS PETERNAKAN Vol 7 No 1 (2020): Juni
Publisher : Universitas Nusa Cendana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35508/nukleus.v7i1.2256

Abstract

The purpose of this study was to determine the effect of giving pituitary extract (EH) and probiotics ABG-O on piglets crossing to increase weaning weight, linear size of body and blood metabolites. The research material was a crossbred of landrace and duroc aged 2 weeks, as many as 24 birds. The experimental design was a completely randomized design with three treatments and eight replications. The three treatments are P0 (control), P1 (EH injection 1 mL), and P2 (injection EH 1 mL + probiotic ABG-O 2 mL). EH injections are carried out every 5 days and administration of probiotics is given orally using spoit every day until the piglets are weaned. The parameters observed were weight gain, linear size of body and blood metabolites. This data was analyzed by ANOVA and continued with Duncan test. The results of this study indicate that the average daily weight gain of crossbred piglets in treatments P0, P1, and P2 are 0.11; 0.13; 016 kg, body length (0.40; 0.45; 0.44 cm), chest circumference (0.29; 0.27; 0,30 cm), and shoulder height (0.25; 0.24; 0.25 cm). Blood glucose levels (117.31; 112.85; 117.25) and total plasma protein (4.60; 4.90; 4.80). The results of the statistical analysis showed that the EH and ABG probiotic treatments affected the UN (P<0.05) but did not affect (P> 0.05) the size of the linear body and blood metabolites. It can be concluded that the administration of pituitary extracts and ABG probiotics can increase the weaning weight but does not affect the linear size of the body blood metabolites and total protein plasma of crossing piglets.
PENGARUH PENAMBAHAN VIRGIN COCONUT OIL DALAM PENGENCER TRIS KUNING TELUR TERHADAP KUALITAS SPERMATOZOA SAPI BALI SELAMA PRESERVASI (Infiluence addition virgin coconut oil in tris egg yolk on the quality of bali bull spermatozoa during preservation) Jernih Blegur; Wilmientje M Nalley; Thomas Mata Hine
JURNAL NUKLEUS PETERNAKAN Vol 7 No 2 (2020): Desember
Publisher : Universitas Nusa Cendana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35508/nukleus.v7i2.2997

Abstract

The purpose of this study was to examine the effect of adding various doses of virgin coconut oil to the quality of bali bull spermatozoa during preservation. Semen is accommodated twice a week using an artificial vaginal method from two 3-4 year old bali bull with normal body condition and reproductive organs. Semen with motility> 70%, concentration> 1000 × 106, and abnormality <15% was diluted with Tris-egg yolk (T-KT) diluent added with virgin coconut oil (VCO) at a concentration of: 0% (T0), 2 % (T1), 4% (T2), and 6% (T3). The diluted semen was stored at 3-5ᶱC. Evaluated every 24 hours for motility, viability, abnormalities and vitality of spermatozoa up to a motility of at least 40%. The results showed that semen diluted with T-EY with 6% VCO (T3) had a higher quality (P <0.05) compared to the other three treatments, with motility reaching (43.10 ± 2 , 83%), viability (48.68 ± 1.59%), abnormalities (5.92 ± 0.90%) and survival (6.00 ± 0.00 days). The conclusion of this study is the addition of 6% virgin coconut oil in Tris-egg yolk diluent is more effective in maintaining the quality of bali cattle liquid semen.
PENGARUH PENAMBAHAN SARI WORTEL DALAM PENGENCER SITRAT KUNING TELUR TERHADAP KUALITAS SPERMATOZOA KAMBING BLIGON (The effect of carrot juice supplementation in citrate - egg yolk extender on spermatozoa quality of bligon goat) Maria Epifania Barek; kirenius uly; Thomas Mata Hine; Wilmientje Marlene Nalley; Henderiana L.L. Belli
JURNAL NUKLEUS PETERNAKAN Vol 7 No 2 (2020): Desember
Publisher : Universitas Nusa Cendana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35508/nukleus.v7i2.3152

Abstract

This study aims to determine the effect of adding various doses of carrot juice into citrate-egg yolk diluents (C-EY) on bligon buck semen quality. Semen was collected twice a week using an artificial vaginal method of three buck with normal body condition and reproductive organs. Semen was 70% of motility, konsentration ≥1000x106mL and abnormality ≤ 15 diluted with C-EY added with carrot juice at a concentrations: 0% (CJ-0), 10% (CJ-10), 12.5% (CJ-12,5), 15% (CJ-15), 17.5% (CJ-17,5), and 20% (CJ-20), and stored in a refrigerator at 3-5o C. The quality of spermatozoa was evaluated every 24 hours. The Variables measured were spermatozoa motility, viability, and abnormalities. The results showed that spermatozoa preserved by C-EY supplemented by 17.5% carrot juice (CJ-17.5) had a higher quality (P<0.05) compared to the other four treatments, with motility (49.04 ± 1.0%), viability (55.70 ± 1.22%), dan abnormalities (4.57 ± 0.88 %) on the 4th day of storage. This study concludes that 17.5% carrot juice into citrate eggyolk extender improves the bligon buck semen
PENGARUH WAKTU EKUILIBRASI TERHADAP KUALITAS SEMEN BEKU BABI LANDRACE DALAM PENGENCER DURASPERM TERMODIFIKASI (Effect of equilibration time on the quality of landrace boar frozen semen in modified durasperm extender) Sofia Marlize; Thomas M. Hine; Wilmientje M. Nalley
JURNAL NUKLEUS PETERNAKAN Vol 8 No 2 (2021): Desember
Publisher : Universitas Nusa Cendana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35508/nukleus.v8i2.4867

Abstract

This study aims to determine the effect of equilibration time on the quality of landrace spermatozoa both pre- and post-freezing and to find the best equilibration time. The materials used is fresh semen collected from a 2,5 years old landrace boar. Collecting was carried out twice per week using the glove hand method. Semen of good quality (sperm motility ≥70%, sperm concentration ≥200 x 106 cells/ml, and percentage of sperm abnormalities ≤15%) was diluted with durasperm that had been modified with lontar fruit juice, while the cryoprotectants used glycerol and sucrose. Holding time was carried out for 2 hour at 27-28°C, centrifuged at 2000 rpm for 15 minutes, and the sperm sediment was diluted with the same diluent. After that, the liquid semen was packed in a 0.5 mL straw and equilibrated at 3-5°C for 1 (P1), 2 (P2), or 3 hours (P3). The freezing of semen was carried out on the surface of liquid nitrogen at a distance of 5 cm for 10 minutes, and then stored in a liquid nitrogen container. Thawing is done by placing the frozen semen straws in warm water (37°C) for 30 seconds. The research data were analyzed with analysis of variance and continued with the Duncan test. The results showed that the equilibration time at 2 hours (P2) gave the best results (P<0,05) with the mean percentage of motility, viability, abnormality, intact plasma membrane and recovery rate respectively were 30,62%, 58,21%, 4,68%, 58,94% and 43,74%. It was concluded that the equilibration time did not affect the quality of pre-freezing spermatozoa, but it did affect the quality of post-freezing spermatozoa with the best equilibration time to maintain the quality of frozen semen of landrace boar was 2 hours.
KUALITAS SEMEN BEKU BABI DUROC DALAM PENGENCER TRIS MODIFIKASI DENGAN WAKTU EKUILIBRASI YANG BERBEDA (The frozen sperm quality of duroc pig in modified tris diluent with different equilibration time) Selsiana Dongkot; Aloysius Marawali; Thomas M. Hine; Wilmientje M. Nalley
JURNAL NUKLEUS PETERNAKAN Vol 9 No 1 (2022): Juni
Publisher : Universitas Nusa Cendana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35508/nukleus.v9i1.5491

Abstract

This study aimed to examine the quality of frozen sperm of duroc boar in modified Tris diluent with different equilibration time. Semen was collected from two duroc males and evaluated macroscopically and microscopically. Semen with motility 75%, concentration 200x106 cell/mL and abnormality <20% was diluted in Tris modified diluent, then held for two hours at room temperature (27-28ºC). The semen that has been in the holding time is then centrifuged at 2000 rpm for 15 minutes. The pellet from centrifuge were diluted again with modified Tris diluent + 3% glycerol. The diluted semen was packed in 0,50 mL mini straws and equilibrated according to the treatment equilibration time, namely P1: 1 hour equilibration time, P2: 2 hour equilibration time, P3: 3 hour equilibration time. After equilibration , it was frozen over liquid nitrogen vapor (-110ºC) at a distance of 5 cm for 10 minutes, then stored in liquid nitrogen (-196ºC) and left for 24 hours. Then thawing with warm water at 37ºC for 30 seconds in a water bath. The result showed that motility, viability, abnormality, intact plasma membrane and recovery rate were significantly higher (P<0,05) at two hour equilibration , namely 23.12%, 45.44%, 4.65%, 50,15%, 33.03% respectively compared to one and two hour equilibration time, namely 20%, 42.17%, 8.19%, 42.67%, 28.57% and 16,87%, 36.39%, 7.64%, 37.73%, 24.99% respectively. This study conclude that the equilibration time of two hours resulted in higher quality of frozen sperm of duroc boar than one and three hours.
PENGARUH KOMBINASI SUSU KACANG KEDELAI DAN PENGENCER SITRAT KUNING TELUR TERHADAP KUALITAS SEMEN SAPI BALI (Effect of combination soy bean milk and citrate egg yolk liqid on the quality of bali cattle semen) Maria E.E. Fernandez; Wilmientje M Nalley; Franky M.S. Telupere; Thomas M. Hine
JURNAL NUKLEUS PETERNAKAN Vol 9 No 2 (2022): Desember
Publisher : Universitas Nusa Cendana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35508/nukleus.v9i2.7949

Abstract

One of the diluent containing lipoproteins and lecithins that function in maintaining and protecting the integrity of the plasma membrane of spermatozoa from cold shock is soy milk. The purpose of this study was to determine the effect of the combination of soy bean milk and egg yolk citrate liqud on the quality of liquid semen of bali cattle. The material used in this study was fresh semen of 3 year old bali cattle. Semen was diluted with egg yolk citrate diluent with a combination of soy bean milk, namely T0: 100% citrate egg yolk (CEY); T1: CEY 75%+soy bean milk (SBM) 25%; T2: CEY 50%+SBM 50%; T3: CEY 25%+SBM 75%; and T4: 100% SBM. Liqud semen was stored at a temperature of 3-5oC. The parameter studied were motility, viability, abnormality and liveability of spermatozoa. The research data ware analyzed using an analysis of variance (ANOVA) and followed by the multiple range test. The results showed that T1 significantly difference (P<0.05) from other treatments, with motility of 42.40±2.50%, viability 52.15±6.31%, abnormality 6.44±0.84%, and liveability 6.00±0.00 days. From these result it could be concluded that the combination of 25% soy bean milk and 75% egg yolk citrate liqud was the best treatment, and spermatozoa can be stored until six days.
Co-Authors A. Kaka A. Parakkasi A. Parakkasi Abdullah Baharun Alfred Nubatonis Aloysius Marawali Aloysius Marawali Aloysius Marawali Aloysius Marawali Arief Boediono Ayang C. Tafuli Banamtuan, A. N. Bayu Rosadi Bette, Yustiany Y Bire Lawa, Alvrado Burhanuddin * Burhanuddin . C. Tafuli, Ayang D. J. Djawapatty Doda Dengi, Donatus Donatus Doda Dengi Dondin Sajuthi Dui, Theresia A. A. T. Enny T Setiatin Enny T Setiatin, Enny Franky M S Telupere George Valentino Kosat Grasele, Fransiskus Allianus Gue, Febronia Dhenge Gunawan G Gunawan G, Gunawan Henderiana Belli Henderiana L.L Belli Heri Armadianto Heri Armadianto Hilly, Fanny Tesalonika Honin, Olfi Mariana Iman Supriatna Ina Mato, Sefania J A Tamoes Jernih Blegur Johanis Ly Kihe, Johny Nada Kii, Mariana B.I. Kirenius Uly Kirenius Uly kirenius uly Kirenius Uly Kristina Meldiana Hayati Kurnia Wawang, Silviani L. L Belli, Henderiana L. L Belli, Henderiana Lawa, Alvrado B Mardemus Misa Maria E.E. Fernandez Maria Epifania Barek Maria Orsella Rindiani Mariana Nenobais Maritje Aleonor Hilakore Masae, Sinoptis Melkiur Nalley, Marlene Nalley, W. Marlene Nalley, Welmince M. Nalley, Wilmientje M Nalley, Wilmientje M. Nalley, Wilmientje Marlen Ni Made Paramita Setyani Nuba, Maria Deran Odo, Remigius Abha Pandahuki, Fransiska Onya Papituan, Mardan Laes Petrus Kune Petrus Kune Petrus Kune Petrus Kune Petrus Kune Riwu, Agustinus R. Sefania Ina Mato Selsiana Dongkot Setyani, Ni M P Siena, Sofia M.S. Sofia Marlize Soleman Manaze Weiri Sinda Stef Amiron Ratu Tapatab, Kelvin Alberto Imanuel Telupere, Franky M S Toto, Novaldy Imanuel Tree May Thiessen Outang Twen O. Dami Dato Ulu, Krista Florida Uly, Kirenius W. M. Nalley W. Marlene Nalley W. Marlene Nalley Wilmientje M. Nalley Wilmientje Marlen Nalley Wilmientje Marlene Mesang Nalley Wilmientje Marlene Nalley, Wilmientje Marlene Wilmintje Marlene Nalley Wirona Adu Yohanes Paulus Martinus Bay Yohanis Umbu Daku Waluwanja Yustiani Yuliana Bette Yustiany Yuliani Bette Yusuf Sae