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All Journal HAYATI Journal of Biosciences WARTAZOA Indonesian Bulletin of Animal and Veterinary Sciences Jurnal Penelitian Pendidikan IPA (JPPIPA) Syntax Literate: Jurnal Ilmiah Indonesia Jurnal Pengabdian Magister Pendidikan IPA Journal of Aquaculture and Fish Health Jurnal Gema Ngabdi Jurnal Abdi Insani Jurnal Warta Desa (JWD) Journal of Fisheries & Marine
Muhamad Ali
Fakultas Peternakan Universitas Mataram
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Earth & Planetary Sciences Economics, Econometrics & Finance Education Engineering Environmental Science Health Professions Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Mathematics Physics Social Sciences Veterinary Other

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Penggunaan Sinbiotik Untuk Meningkatkan Pertumbuhan Ayam Pedaging Di Peternak Muara Selayar Desa Pijot Kabupaten Lombok Timur Muhamad Ali; Djoko Kisworo; Embun Suryani
Jurnal Gema Ngabdi Vol. 4 No. 3 (2022): JURNAL GEMA NGABDI
Publisher : Universitas Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jgn.v4i3.282

Abstract

Broiler cultivation is one of the largest MSMEs in East Lombok Regency, West Nusa Tenggara Province (NTB). The group of breeders carries out maintenance on a scale between 3,500 heads and 7,500 heads per cage. The Muara Selayar farmer group is one of the broiler farmer groups located in Pijot Village, Keruak District, East Lombok Regency. The main problem faced by the Muara Selayar Farmer Group at this time is that production performance is not optimal, which is characterized by sluggish chicken growth, many dwarf chickens, poor feed conversion, high mortality rate, which results in low profits obtained and even losses. The considerable number of dwarf chickens although it varies every period incurs losses due to poor ration conversion with low selling prices. The solution offered to bridge the problem of low broiler production performance in the Muara Selayar Broiler Farm Group is the use of symbiotic as a feed mixture for broilers of the starter period. Symbiotic are a mixture of prebiotics (feed ingredients that are difficult to digest in the digestive tract but benefit the host through selective stimulation of growth or activity of one or more microbes in the gut) and probiotics (live microbes that have benefits for the host). The results of this activity show that the use of symbiotic has succeeded in increasing the body's resistance and growth of slaughter chickens and laying hens.
DISEMINASI PENERAPAN TEKNOLOGI AUDIO ON DEMAND MELALUI PODCAST SEBAGAI MEDIA INFORMASI DI ERA INDUSTRI 4.0 Halil Akhyar; Deasy Mariyani; Susi Rahayu; Muhamad Ali
Jurnal Abdi Insani Vol 9 No 3 (2022): Jurnal Abdi Insani
Publisher : Universitas Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/abdiinsani.v9i3.633

Abstract

Information technology is a technology utilized to create, modify, communicate or disseminate up-to-date information through an advanced media. Podcast is the current channel to spread out an informative and educational information. The popularity of podcasts as a public medium to deliver information increases significantly in the recent year. This is supported by the condition of the Covid-19 pandemic. The podcast is continuously applicated in all areas, such as entertainments, governments and educational circumstances. The two-way communication has become an interactive broadcasting system not only for the young people but also for the elderly people. So that the presence of podcasts continues to be positively welcomed by the community. The event aims to form a podcast as a media for promotion and dissemination of information related to research and service innovations produced by lecturers at the University of Mataram. It is carried out in some stages, including surveying the situation and field conditions, implementating, and evaluating. In terms of the implementation phase, it is divided into three main activities, such as socializing, training, and mentoring. To facilitate these activities, several approaches were applied, like participatory rural appraisal (PRA), persuasive approaches, and educational approaches. The results of the survey conducted by the health promotion team from the health department of West Nusa Tenggara Province showed that LPPM required several equipment, such as cameras/webcams, microphones, earphones, a mixer, tripods, lightings, an OBS software, an Adobe Premier Pro, and a personal computer. In addition, from the results of observations, it is concluded that the Research and Service Institute (LPPM) of the Mataram University has a suitable room to hold the podcast. Overall, the implementation of podcast at the LPPM has been successfully implemented. The evaluation as one of the most important part shows that there are technical and non-technical obstacles occurred, such as none-standardized computer equipment and room arrangements. This is an important note for the next implementation. However, in general, the podcast as an informative, educative, and accurate information and promotion media has been successfully established at the LPPM of Mataram University.
Enhancing Solubility of Recombinant Plasmodium Lactate Dehydrogenase (pLDH) Using Combination of Cold-Inducible Expression System and Cold-Stirred Bioreactor Alimuddin; Muhamad Ali; Sahrul Alim; Muhamad Amin
HAYATI Journal of Biosciences Vol. 30 No. 3 (2023): May 2023
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.30.3.561-566

Abstract

A major drawback associated with an expression of a high-level Plasmodium Lactate Dehydrogenase (pLDH) using Escherichia coli is the low solubility due to the formation of an inclusion body (IB). This study aimed to develop a suitable protocol for enhancing the solubility of pLDH expressed in E. coli. Firstly, a pLDH-encoding gene was amplified from the blood sample of malaria-infected patients and ligated into pBlueScript II KS+ for sequencing. Afterward, the pLDH gene was digested and cloned into pColdTF for expression. The recombinant plasmid was transformed into the E. coli BL21 (DE3) RIPL Codon Plus Strain. Then, the bacterial host was initially cultured at 37°C until reaching optical density (OD) at 600 nm: 0.5. Thereafter, the growth temperature was lowered to 15°C, followed by the addition of 0.1 mM IPTG into the culture medium for inducing pLDH expression. Thereafter, the bacterial hosts were cultured in a cold-stirred bioreactor (15°C). The result showed that a combination of the low culture conditions (15°C) and a low amount of IPTG increased the solubility of pLDH. This result suggests that this protocol can be a convenient method for generating high-quality recombinant protein using the E. coli system.
Isolation of Cellulolytic Bacteria from Kalkun (Meleagris gallopavo) Gastro-Intestinal Tract as a Candidate Probiotics for Poultry Muh. Aidil Fitriyan Fadjar Suryadi; Muhammad Hipzul Mursyid; Khairil Anwar; Muhamad Ali; Djoko Kisworo
Jurnal Penelitian Pendidikan IPA Vol 9 No 5 (2023): May
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v9i5.3739

Abstract

This study has been conducted to isolate and identify cellulolytic bacteria from the digestive tract of turkeys (Meleagris gallopavo) as probiotic candidates for poultry. This study has obtained 4 bacteria that have the potential as probiotic candidates through the morphological identification stage, the ability to produce cellulase enzymes and resistance to acidic pH conditions. The test found that RDL code bacteria bacteria have the highest ability to produce enzyme cellulase characterized by the formation of a clearing zone with a diameter of 11 mm, the second highest PVB2 code bacteria with a clearing zone diameter of 7 mm, DB1 4 mm, and RSL 2 mm and the results of bacterial viability tests isolated at low pH (2.0) showed the highest viability after incubating for 4 hours. RDL viability is 65%, RSL 48%, DB1 50%, and PVb2 29%. So based on the tests that have been carried out, it is estimated that the 4 bacteria are candidates for Lactobacillus sp. worthy to be used as a probiotic for poultry
IDENTIFIKASI PENYEBAB KEMATIAN DAN KEKERDILAN PADA AYAM RAS PEDAGING DI KELOMPOK PETERNAK MUARA SELAYAR DESA PIJOT KECAMATAN KERUAK KABUPATEN LOMBOK TIMUR Made Sriasih; Sulaiman Ngongu Depamede; Wayan Wariata; Muhamad Ali; Anwar Rosyidi; Aini Aini
Jurnal Abdi Insani Vol 10 No 2 (2023): Jurnal Abdi Insani
Publisher : Universitas Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/abdiinsani.v10i2.488

Abstract

Success in broiler farming depends on several factors, namely feed, day-old chick (DOC), poultry management, and disease attacks. The Muara Selayar broiler farmer group in Pijot village in the Keruak sub-district of East Lombok is one of the non-industrial poultry farmers. The problems faced by the Muara Selayar farmer group are the mortality rate of DOC, which reached 7.4% (above the 5% threshold), and stunting syndrome. This community service activity aimed to identify the causes of the problems faced by the group so that losses experienced by farmers could be minimized. This community service activity was carried out through direct identification of dead chickens (post-mortem identification), surveys, and discussions. Based on the identification results, the cause of death of chickens in the Muara Selayar group was coccidiosis, characterized by reddish feces and bleeding in the cecum. Stunting syndrome in livestock is mainly caused by poor quality (grade) of seeds from the partners. The results of the discussion activities conducted with the farmers showed that they need bargaining power with partners about the quality of the DOC, so it is necessary to have special communication with partners who cooperate with them. Farmers must pay attention to good husbandry practices such as sanitizing flocks and the surrounding environment and treating livestock with safe materials that do not cause drug residues. From this community service activity, it can be concluded that coccidiosis was the cause of the death of chickens in the Muara Selayar farmer group. However, other poultry diseases such as Pullorum, ND, and CRD should receive attention because they often appear during the rearing period based on farmer reports. Stunting syndrome is mainly caused by low DOC quality.
Isolasi dan Identifikasi Bakteri Asam Laktat Penghasil Enzim Fitase sebagai Kandidat Probiotik Untuk Ternak Unggas Zaid Al Gifari; Khairil Anwar; Anwar Rosyidi; Muhamad Ali; Muhamad Amin
Syntax Literate Jurnal Ilmiah Indonesia
Publisher : Syntax Corporation

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (287.27 KB) | DOI: 10.36418/syntax-literate.v7i6.8369

Abstract

Probiotik merupakan mikroorganisme hidup yang bersifat non-patogenik dan non-toksik yang menguntungkan bagi inang. Bakteri asam laktat (BAL) merupakan bakteri probiotik yang digunakan secara luas pada ternak unggas dan memiliki keunggulan dalam menghasilkan enzim ekstraseluler. Penelitian ini bertujuan untuk melakukan isolasi, identifikasi dan karakterisasi bakteri asam laktat penghasil enzim fitase yang bersumber dari saluran pencernaan entok (Cairina moschata). Berdasarkan produksi enzim fitase, 5 isolat bakteri yaitu AL01, RIL07, FA16, AN32, dan NS05 teridentifikasi menghasilkan enzim fitase yang ditandai dengan dengan terbentuknya clearing zone.
Isolasi, Identifikasi, dan Karakterisasi Pediococcus spp. dan Lactobacillus spp. dari Saluran Pencernaan Entok (Cairina moschata) Sebagai Kandidat Probiotik Unggas khairunnisah khairunnisah; Fatimah Azh Zhahro Bagis; Fitri Andriani; Khairil Anwar; Zaid AL Gifari; Anwar Rosyidi; Muhamad Ali
Syntax Literate Jurnal Ilmiah Indonesia
Publisher : Syntax Corporation

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (358.703 KB) | DOI: 10.36418/syntax-literate.v7i12.10835

Abstract

Probiotik merupakan mikroorganisme hidup yang dapat berguna untuk meningkatkan produktivitas ternak baik kecernaan pakan maupun kesehatan. Saluran pencernaan merupakan salah satu sumber probiotik. Bakteri asam laktat (BAL) merupakan mikroorganisme yang banyak dimafaatkan sebagai probiotik untuk ternak unggas dan mempunyai keunggulan mampu menghasilkan enzim ekstraseluler. Penelitian ini dilakukan dengan mengisolasi bakteri dari saluran pencernaan Entok (Cairina moschata) untuk mendapatkan bakteri asam laktat dari genus Pediococcus spp. dan Lactobacillus spp. sebagai kandidat probiotik unggas. Isolasi dilakukan dengan mengambil isi dalam pada saluran pencernaan kemudian diencerkan dengan pengenceran bertingkat dan dibiakkan dengan metode pour plate pada media MRS. Kemudian dipilih koloni bakteri yang berbeda untuk isolasi lebih lanjut. Isolat bakteri diambil dari 3 sumber yakni proventrikulus, duodenum, dan ileum. Identifikasi dilakukan secara morfologi dan fisiologi. Identifikasi morfologi meliputi identifikasi koloni bakteri dan sel bakteri. Identifikasi secara fisiologi dilakukan dengan uji katalase, pembentukan gas H2S, produksi indol, motilitas, dan kemampuan memfermentasi karbohidrat. Kakteristik bakteri Pediococcus spp. dan Lactobacillus spp. sebagai kandidat probiotik unggas dilihat kemampuan menghasilkan enzim fitase dan protease, serta aktivitas antimikroba patogen Escherichia coli ATCC 25922 dan Staphylococcus aureus. Berdasarkan hasil identifikasi secara morfologi, fisiologi, kemampuan dalam menghasilkan enzim fitase dan protease, serta aktivitas antimikroba 5 isolat untuk identifikasi berdasarkan gen sekuen 16S rRNA. Hasil sekuensing menunjukkan isolat D3 merupakan Pediococcus pentosaceus strain AM26, isolat P2 merupakan Pediococcus pentosaceus strain L1, dan isolat M1S dan M8S merupakan Lactobacillus salivarius strain RBL73, serta M3S merupakan Lactobacillus salivarius strain 2968.
Synthesis and Characterization of Nano Chitosan from Vannamei Shrimp Shell (Litopenaeus vannamei) Dian Wijaya Kurniawidi; Siti Alaa; Eva Nurhaliza; Desti Olga Safitri; Susi Rahayu; Muhamad Ali; Muhamad Amin
Jurnal Ilmiah Perikanan dan Kelautan Vol. 14 No. 2 (2022): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.v14i2.32864

Abstract

Highlight Research New method to synthesis nano chitosan from Vannamei shrimp with high Deacetylation Degree Nano chitosan from Vannamei shrimp can adsorb heavy metal Fe up to 81.35% Shrimp shells as an alternative adsorbents for Fe   Abstract Shrimp cultivation produces shrimp wastes in several forms, including shells from the molting process. Shrimp shell waste can be used as a source of nano chitosan. Many researches have used nano chitosan for various applications, one of which is to adsorb heavy metal pollution. This present study aimed to extract chitosan from shrimp shells and investigate the ability of nano chitosan to adsorb Fe metal. The research began by isolating chitin and chitosan from shrimp shells through deproteinization, demineralization, and deacetylation. The obtained chitosan was afterward characterized using Fourier Transform Infrared (FTIR). Furthermore, the glassy ionic method was used to synthesize nano chitosan. Nano chitosan was characterized using a scanning electron microscope (SEM), and the Fe metal adsorption ability was measured using Atomic Absorption Spectroscopy (AAS). The FTIR results showed that the synthesized shrimp shells had successfully formed chitin compounds with the appearance of the C-O-C functional group, while the chitosan compounds with the appearance of O-H and N-H groups. The SEM characterization showed that nano chitosan with a size of 173.71 nm was successfully formed with identical morphology in the form of a flat (spherical), elongated, and irregular position. The nano chitosan was able to absorb Fe solution up to 81.35%. Therefore, nano chitosan from the shrimp shells is suitable as an adsorbent of heavy metal Fe.
Co-Authors aini aini aini Ali Haris Alimuddin Anwar Rosyidi Anwar Rosyidi Arafatul Ulfa Bagus Dwi Hari Setyono Dara Puspita Anggraeni Deasy Mariyani Desti Olga Safitri Dian Wijaya Kurniawidi Diswandi Djoko Kisworo Djoko Kisworo Eva Nurhaliza Fatimah Azh Zhahro Bagis Fitri Andriani Halil Akhyar Hilalia Jihad Akbar Khairil Anwar Khairil Anwar Khairil Anwar Khairil Anwar khairunnisah khairunnisah L. Muhammad Furkan Lalu Muhammad Furkan M Ashari Made Sriasih Mahfud Akbar Muh. Aidil Fitriyan Fadjar Suryadi Muhamad Amin Muhammad Hipzul Mursyid Muhammad Zubair Muhdin Muhdin Nefi Andriana Fajri Novia Anggraeni Sahrul Alim Sarifudin Serip Serifudin Serif Siti Alaa Sri Wahyulina Sulaiman Ngongu Depamede Suryani, Embun Susi Rahayu Wariata, Wayan Zaid Al Gifari