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RESPONS FISIOLOGIS DAN DAYA TAHAN SAPI PERANAKAN ONGOLE DAN SAPI BRAHMAN CROSS TERHADAP CEKAMAN PANAS DI KPT MAJU SEJAHTERA TANJUNG SARI LAMPUNG SELATAN Adhitia, Fabian; Qisthon, Arif; Husni, Ali; Hartono, Madi
Jurnal Riset dan Inovasi Peternakan (Journal of Research and Innovation of Animals) Vol 6 No 3 (2022): Jurnal Riset dan Inovasi Peternakan: Agustus 2022
Publisher : Department of Animal Husbandry, Faculty of Agriculture, University of Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jrip.2022.6.3.300-304

Abstract

Penelitian ini bertujuan untuk mengetahui respons fisiologis dan daya tahan ternak sapi Peranakan Ongole (PO) dan sapi Brahman Cross (BX) terhadap cekaman panas di lokasi penelitian. Penelitian ini dilaksanakan di KPT Maju Sejahtera, Kecamatan Tanjung Sari, Kabupaten Lampung Selatan, Provinsi Lampung, yang terdiri dari 6 kelompok yaitu Bumi Asih Sejahtera, Karya Makmur, Sudi Makmur 3, Fajar Jaya, Barokah Jaya, dan Jaya Abadi. Penelitian ini berlangsung dari Bulan September - Oktober 2021. Data penelitian menggunakan data primer dan data sekunder. Sampel yang digunakan sebanyak 66 ekor sapi betina BX dan PO yang ditentukan dengan purposive sampling. Peubah yang diamati ialah respons fisiologis ternak meliputi suhu rektal, frekuensi pernafasan, frekuensi denyut jantung, dan indeks daya tahan panas, serta iklim mikro kandang yang meliputi, suhu udara, kelembaban relatif (RH), dan Temperature Humidity Index (THI). Hasil penelitian ini menunjukkan rataan THI di lokasi penelitian sebesar 83,58. Rataan frekuensi respirasi siang hari ternak sapi PO dan BX berturut-turut yaitu 33,50 ± 3,84 kali/menit dan 34,47 ± 5,20 kali/menit. Rataan frekuensi denyut jantung siang hari ternak sapi PO dan BX berturut-turut yaitu 70,65 ± 10,57 kali/menit dan 76,19 ± 10,30 kali/menit. Rataan suhu rektal siang hari sapi PO dan BX berturut-turut yaitu 38,58 ± 0,23 oC dan 38,92 ± 0,37 oC. Rataan daya tahan panas sapi betina PO dan BX berturut-turut adalah sebagai berikut 2,36 ± 0,25 dan 2,67 ± 0,24. Dari penelitian ini dapat disimpulkan sapi PO memiliki daya tahan panas yang lebih baik dari sapi BX.
Peran Kepala Sekolah Dalam Pemberdayaan Komite Sekolah Di SMK Al-Asy’ari Komis Kedungdung Sampang Badruttamam, Badruttamam; Husni, Ali
AL -ALLAM Vol. 6 No. 2 (2025): JULI
Publisher : LP2M Institut Agama Islam Nazhatut Thullab Sampang

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Abstract

The school committee is an institution that plays a vital role in increasing the responsibility and role of the community in decision-making at school, as a provider of consideration in the implementation of educational policies, as a supporter, and as a controller. Therefore, the school committee needs to be empowered to be more effective in carrying out its duties and functions. This thesis discusses the role of the principal in empowering the school committee at SMK Al-Asy'ari Komis Kedungdung Sampang. There are three research focuses that are the main study in this study: First, What is the role of the principal in empowering the school committee at SMK Al-Asy'ari Komis? Second, How does the principal manage the empowerment of the school committee at SMK Al-Asy'ari Komis? Third, What are the obstacles faced by the principal in empowering the school committee at SMK Al-Asy'ari Komis? This study uses a descriptive qualitative approach. Data sources were obtained through observation, interviews, and documentation. The results of this study indicate that: the principal's role in empowering the school committee at SMK Al-Asy'ari Komis uses a participatory approach, also involving alumni, guardians, committee administrators, and the Islamic boarding school in all stages of the program. Monthly forums are used for communication and collaborative problem-solving with community input, so that the program belongs to all parties involved. The principal's management of the school committee empowerment at SMK Al-Asy'ari Komis is aspirational, but direct communication remains a barrier. The principal of SMK Al-Asy'ari Komis in empowering the school committee is the lack of activity of children who live far from their parents. Limited control and communication make it difficult to achieve learning quality standards.
Molecular diversity of citrus genotypes using callose synthase 7 gene markers linked to Huanglongbing resistance Nugroho, Kristianto; Purwito, Agus; Sukma, Dewi; Kosmiatin, Mia; Santoso, Tri Joko; Husni, Ali; Martasari, Chaireni; Lestari, Puji
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 53 No. 2 (2025): Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy)
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24831/jai.v53i2.64952

Abstract

Huanglongbing (HLB), caused by Candidatus Liberibacter asiaticus, is a notable disease affecting citrus plantations globally. Several studies showed that the callose synthase 7 gene is crucial for the citrus defense system against this pathogen. The study aimed to analyze the nucleotide variations and genetic diversity among several citrus genotypes using specific gene primers designed from the callose synthase 7 gene sequence. Genomic DNA from eleven citrus genotypes was amplified using the specific primers, and Sanger sequencing was employed to identify the nucleotide sequence of the PCR products. The results revealed a total of 66 single-nucleotide polymorphisms (SNPs), 10 insertions, and 11 deletions were detected in callose synthase 7 gene fragment sequences. Of these, one out of five noteworthy SNPs identified at a position of 200 bp downstream of the START codon showed distinguishing features between susceptible and resistant/tolerant genotypes. Phylogenetic analysis clearly discriminated the eleven citrus genotypes into two clusters at a dissimilarity coefficient of 0.05, with all genotypes grouped in the first cluster, except for the Chinese box orange and orange jasmine. The identification of notable SNPs in this study can aid in developing new markers for the rapid selection of genotypes with enhanced HLB resistance in citrus breeding programs.   Keywords: Candidatus Liberibacter asiaticus; nucleotide variations; Sanger sequencing; phylogenetic analysis; SNP
PENGARUH PEMBERIAN SILASE KULIT PISANG TERHADAP PERFORMA DAN KONVERSI RANSUM PADA DOMBA EKOR TIPIS Maliya, Mirwa Siti; Adhianto, Kusuma; Liman, Liman; Husni, Ali
Jurnal Riset dan Inovasi Peternakan (Journal of Research and Innovation of Animals) Vol 9 No 3 (2025): Jurnal Riset dan Inovasi Peternakan: Agustus 2025
Publisher : Department of Animal Husbandry, Faculty of Agriculture, University of Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jrip.2025.9.3.564-573

Abstract

This research aims to determine the best treatment for giving banana peel silage on body weight gain, ration consumption and ration conversion. This research was carried out in December 2023 ̶ February 2024 located at the Animal Husbandry Department, Faculty of Agriculture, Lampung University, Bandar Lampung. This research was carried out using a Randomized Block Design (RAK) consisting of 3 treatments and 5 replications, using 15 thin-tailed sheep. The treatment used is P0: 50% concentrate + 50% tebon silage; P1: 50% concentrate + 35% tebon silage + 15% banana peel silage; P2: 50% concentrate + 20% corn stalks silage + 30% banana peel silage. The data obtained were analyzed using Analysis of Variance (ANOVA). The results of the research on daily body weight gain were (P0 50,57±29,78; P1 90,19±11,03; P2 62,64±32,31) gr/day, on ration consumption of (P0 1118,18±71,06; P1 1252,47±241,82; P2 1322,82±138,50) gr/day, and at ration conversion of (P0 25,38±14,46; P1 14±2,71; P2 25,89±14,69). Providing banana peel silage in treatment P1 had the best effect on daily body weight gain and feed consumption. The P2 treatment had the best effect on feed conversion.
PENDUGAAN BOBOT TUBUH SAPI SIMPO MENGGUNAKAN UKURAN TUBUH DAN VOLUME TUBUH DI KELOMPOK TERNAK LIMOUSIN LIVESTOCK KECAMATAN PUNGGUR KABUPATEN LAMPUNG TENGAH Musthofa, Arfan Fahrizki; Dakhlan, Akhmad; Husni, Ali; Adhianto, Kusuma
Jurnal Riset dan Inovasi Peternakan (Journal of Research and Innovation of Animals) Vol 9 No 3 (2025): Jurnal Riset dan Inovasi Peternakan: Agustus 2025
Publisher : Department of Animal Husbandry, Faculty of Agriculture, University of Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jrip.2025.9.3.612-625

Abstract

Penelitian ini bertujuan untuk menduga bobot tubuh sapi Simpo menggunakan ukuran-ukuran tubuh dan volume tubuh yang dihitung dari panjang badan dan lingkar dada. Penelitian ini dilaksanakan di Kelompok Ternak Limousin Livestock Kecamatan Punggur Kabupaten Lampung Tengah. Metode yang digunakan pada penelitian ini adalah survey. Teknik pengambilan sampel dilakukan dengan cara purposive sampling dengan cara mengukur dan menimbang tubuh sapi Simpo dengan kriteria sapi berjenis kelamin jantan, umur sapi 1-4 tahun, dan bobot tubuh berkisar 200-550 kg. Data yang diperoleh kemudian ditabulasi menggunakan program excel kemudian data panjang badan, lingkar dada, dan volume tubuh dianalisis korelasi dan regresinya dengan bobot tubuh sapi Simpo. Analisis korelasi dan regresi antara variable independen dan variabel dependen menggunakan program R. Hasil penelitian menunjukkan bahwa nilai korelasi antara lingkar dada, panjang badan, dan volume tubuh terhadap bobot tubuh masing-masing yaitu 0,81, 0,87, dan 0,91. Sedangkan persamaan regresi antara panjang badan (PB), lingkar dada (LD) dan volume tubuh (VT) terhadap bobot tubuh masing-masing yaitu BT = -306,8569 + 4,6133PB; BT = -453,0868 + 4,7904LD; dan BT = 68,69 + 0,0008738VT, dengan koefisien determinasi masing-masing adalah 0,66; 0,76; dan 0,82. Kesimpulan penelitian ini adalah bahwa ukuran tubuh dan volume tubuh dapat digunakan untuk menduga bobot tubuh sapi Simpo.
Pengembangan Potensi Lokal dan Penguatan Daya Saing Desa Jarangan Kecamatan Rejoso Kabupaten Pasuruan Melalui Inovasi Sosial, Ekonomi Kreatif, dan Transformasi Digital Ariesta, Wiwin; Syinwani, Billy M; Maulidiyah, Salamatul; Husni, Ali; Kirani, Wahyu Sasi
HIBRUL ULAMA Vol. 7 No. 2 (2025): Jurnal Hibrul’ulama
Publisher : Fakultas Agama Islam Universitas Al Washliyah

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.47662/hibrululama.v7i2.1103

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Jarangan Village is located in the Rejoso District of Pasuruan Regency. According to local government statistics, Jarangan Village is considered one of the least developed villages. However, in terms of its true potential, Jarangan Village possesses sufficient natural resources and human resources to become a thriving village. The community service team selected Jarangan Village as the location for this community service project, demonstrating the academics' commitment to village development. Several programs focused on developing local potential and strengthening competitiveness through social innovation, the creative economy, and digital transformation, all three of which are important but lacking in Jarangan Village, Rejoso District, Pasuruan Regency.
Analisis Korelasi dan Regresi antara Volume Tubuh dengan Bobot Tubuh Kambing Saburai Menggunakan Persamaan Linear (Studi Kasus di Kelompok Ternak Tani Makmur II kecamatan Gisting Kabupaten Tanggamus Provinsi Lampung) Febriyanti, Leni Nur; Dakhlan, Akhmad; Husni, Ali; Qisthon, Arif
Jurnal Riset dan Inovasi Peternakan (Journal of Research and Innovation of Animals) Vol 8 No 4 (2024): Jurnal Riset dan Inovasi Peternakan: November 2024
Publisher : Department of Animal Husbandry, Faculty of Agriculture, University of Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jrip.2024.8.4.729-736

Abstract

Penelitian ini bertujuan untuk mengetahui korelasi dan regresi antara bobot tubuh (BT) dengan volume tubuh (VT) yang dihitung menggunakan panjang badan (PB) dan lingkar dada (LD), pada kambing Saburai betina di Kelompok Tani Ternak II, Kecamatan Gisting, Kabupaten Tanggamus, Provinsi Lampung yang dilaksanakan pada Januari 2023. Ternak yang digunakan dalam penelitian ini merupakan kambing Saburai betina berumur 1—3 tahun sebanyak 33 ekor dan tidak bunting. Metode yang digunakan adalah survei dengan pengambilan data dilakukan secara purposive sampling dengan studi kasus. Data yang diperoleh ditabulasi menggunakan program excel. Data dianalisis dengan analisis korelasi dan regresi linear antara variabel independent (PB, LD, and VT) dan variabel dependen (BT) menggunakan program R. Hasil penelitian menunjukkan bahwa LD, PB, dan VT memiliki korelasi dengan BT kambing Saburai betina, dimana LD memiliki korelasi kuat dengan nilai 0,7586; PB memiliki korelasi yang kuat dengan nilai 0,7419; dan VT memiliki nilai korelasi yang kuat dengan nilai 0,8408. Sedangkan persamaan regresi antara lingkar dada (LD), panjang badan (PB), dan volume tubuh (VT) terhadap bobot tubuh masing-masing yaitu BT = -50,1805 + 1,1924LD; BT = -25,1642 + 0,8728PB; BT = 5,8799 + 0,001VT dengan koefisien determinasi (R²) secara berurutan yaitu 0,6378; 0,5504; dan 0,7070.
Embriogenesis dan Desikasi Embrio Somatik Jeruk Keprok Batu 55 (Citrus reticulata Blanco.) untuk Meningkatkan Frekuensi Perkecambahan Fathur Rahmi, Atika; Purwito, Agus; Husni, Ali; Dinarti, Diny
Jurnal Hortikultura Indonesia (JHI) Vol. 8 No. 2 (2017): Jurnal Hortikultura Indonesia
Publisher : Indonesian Society for Horticulture / Department of Agronomy and Horticulture

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (381.578 KB) | DOI: 10.29244/jhi.8.2.79-87

Abstract

ABSTRACTIn vitro breeding technique of citrus is effective when optimum explant regeneration method is obtained. Low germination frequency and high abnormality were barrier in citrus somatic embryogenesis. This research aimed at optimizing somatic embryogenesis in Tangerine var. Batu 55. This research consisted of 3 experiments. First experiment was maturation of embryogenesis, using Completely Randomized Design (CRD) method. Modified MS+MW was used as basic media added with 500 mg L-1 malt extract (control) and addition of 3 mg L-1 BAP, and 2.5 mg L-1 ABA as treatments. Second experiment was SE (cotyledonary phage) desiccation. Factorial CRD used in two factors. First factor was poly-ethylene-glicol/PEG 8000 (0, 2.5, 5, 7.5 and 10%), while second factor was immersed periods (control, 3, 6, and 9 hours), in desiccant solution (base medium + PEG). Third experiment was studied of plantlet growth and development planlets. Based on CRD 2 factor method, the first factor was PEG concentrations from the second experiment. Second factor were active charcoal treatments (with or without), in basic media. The result showed that 2.5 mg L-1 ABA produced has highest mature somatic embryo (SE). Desiccation for 9 hours, induced the highestt germination frequencies (90.29%). The best growth of plantlets shown in previous experiments immersed desiccant PEG 2.5% for 9 hours, and cultured in basic media with 2 g L-1 of activated charcoal.Keywords: desiccant, embryogenic callus, maturation, PEG 8000, somatic embryo ABSTRAK Pemuliaan tanaman melalui teknik in vitro efektif bila metode regenerasi eksplan optimum telah diperoleh. Rendahnya frekuensi perkecambahan dan tingginya abnormalitas, menjadi kendala pada embriogenesis somatik jeruk. Penelitian terdiri atas 3 percobaan paralel, bertujuan mengoptimalkan metode embriogenesis somatik jeruk, khususnya Keprok Batu 55. Percobaan pertama pematangan kalus embriogenik menggunakan Rancangan Acak Lengkap (RAL) satu faktor, dengan perlakuan penambahan ZPT (kontrol, 3 mg L-1 BAP, dan 2.5 mg L-1 ABA) pada media dasar (MS modifikasi vitamin MW) diperkaya 500 mg L-1 ekstrak malt. Percobaan kedua desikasi embrio somatik (fase kotiledon) menggunakan RAL dua faktor. Faktor pertama konsentrasi poly-ethylene-glicol/PEG 8000 (0, 2.5, 5, 7.5 dan 10%), dan faktor kedua waktu perendaman (kontrol, 3, 6, dan 9 jam) pada larutan desikan (media dasar + PEG). Percobaan ketiga mempelajari pertumbuhan dan perkembangan planlet, menggunakan RAL dua faktor. Faktor pertama konsentrasi PEG planlet pada percobaan kedua, dan faktor kedua perbedaan media dasar (tanpa dan dengan arang aktif). Hasil percobaan menunjukkan penambahan 2.5 mg L-1 ABA menghasilkan maturasi embrio somatik terbaik. Desikasi 9 jam menghasilkan frekuensi perkecambahan 90.29%. Pertumbuhan terbaik ditunjukkan planlet yang pada percobaan sebelumnya direndam 9 jam desikan PEG 2.5%, dan dibesarkan pada media dasar dengan 2 g L-1 arang aktif.Kata kunci : desikan, embrio somatik, kalus embriogenik, PEG 8000, pematangan
Perkecambahan dan Pertumbuhan in vitro Jeruk Nipis (Citrus aurantifolia) Komang Trisna Wirakusuma; Purwito, Agus; Husni, Ali; Kosmiatin, Mia
Jurnal Hortikultura Indonesia (JHI) Vol. 14 No. 1 (2023): Jurnal Hortikultura Indonesia
Publisher : Indonesian Society for Horticulture / Department of Agronomy and Horticulture

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jhi.14.1.1-8

Abstract

In vitro selection to increase the citrus resistance to Huanglongbin-HLB diseases, requires theavailability of lime in vitro shoots as a negative control, because it is one type of citrus that is verysusceptible to HLB. In vitro shoots can be obtained from seed germination in vitro with a more controlledenvironment. Shoots derived from in vitro germination can be used as controls when in vitro artificialinoculation by pathogen suspension is performed to select in vitro breeding lines. This study aims to obtainthe best concentration of gibberellins and scarification treatment for in vitro germination of lime.Germination was carried out on citrus seeds that had been sterilized on the surface of the fruit. Scarificationis done by injuring the testa of the seed. Lime seeds with and without scarification treatment were germinated on MS+VMW base media with the addition of GA3 0 mg L-1, 5 mg L-1, 10 mg L-1, and 15 mgL-1. The results showed that the optimal concentration of GA3 to induce lime seed germination was 5 mgL-1. Scarification by wounding the seed testas significantly accelerated lime germination time. Keywords: Citrus aurantifolia, in vitro selection, negative control, of GA3, scarification
Peningkatan Toleransi Kedelai Sindoro terhadap Kekeringan Melalui Seleksi In Vitro Husni, Ali; Kosmiatin, M.; Mariska, Ika
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 34 No. 1 (2006): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (200.094 KB) | DOI: 10.24831/jai.v34i1.1271

Abstract

In vitro selection of embryogenic cell mass is one alternative to improve drought tolerance in plants. Embryogenic cell callus of soybean were radiated by Gamma ray (400 rad) to produce mutation. The radiated cell  were tested with PEG (0, 10, 20 and 30 %) for drought stress tolerance. After selection, cells which tolerant to PEG were regenerated to produce somatic embryo structure, somatic seed and plantlet. Acclimatization was done in a greenhouse and analysis of proline was done at generation 1 (G1). The purpose of the experiment was to get soybean somatic seed which tolerant to drought stress. Results of experiment showed that 39.7 % embriogenic callus were produced. The higher the concentration of PEG, the higher the death of cell/callus. The rate of producing somatic embryo structure was 4.9 at 0 % PEG; 2.85 at 10 % PEG; 1.6 at 20% PEG and 0.6 at 30% PEG. Number of somatic seed which developed in regeneration medium (S11) were 79 from 0% PEG;  35 from 10% PEG; 29 from 20% PEG, and 15 from 30% PEG. Somatic seed produced 15 planlets from PEG 0%; 6 planlets from PEG10%; 4 planlets from PEG 20%. Based of proline content, all of G1 somaclones were more tolerant  than the mother plant.     Key words : Soybean, in vitro selection, PEG, regeneration, acclimatization and dry land.                     
Co-Authors Adhitia, Fabian Agus Joko Santoso Agus Purwito Annisa, Ajeng Nur Antika, Dea Mela Ariesta, Wiwin Arif Qisthon Arzakiyah, Fina Badruttamam, Badruttamam Baroya, Mushlihatun Beninda, Adellia Budiman, Edy Budiyanto, Ferly Rahmat Chaireni Martasari Dakhlan, Akhmad Daniatur, Arya Desyandri Desyandri Dewi Sukma Dewi, Anggi Derma Tungga Dian Puji Rahayu Dian Septinova Diny Dinarti Disa, Putra Rama edy santosa, purnama Eka Rosyida Erwanto Erwanto Farida Fathul Fathur Rahmi, Atika fatimah Fatimah Febriyanti, Leni Nur Fitri Yulianti Fransiska Hamdani, M Dima Iqbal Hobir, NFN Ika Mariska Ilhamsyah, Alifudin Indonant, Hassem Muhamad Inka Aulia Karsinah - Karyanti ,, Karyanti Kirani, Wahyu Sasi Kohir, Muhammad Zahir Alwan Komang Trisna Wirakusuma KRISTIANTO NUGROHO, KRISTIANTO Kurniantha, Muhammad Aldi Kusuma Adhianto Liman Liman Liman, Liman M. Kosmiatin Madi Hartono Maemunah Maemunah Maliya, Mirwa Siti Maulidiyah, Salamatul Mia Kosmiatin Muhammad Habibi Muhammad Mirandy Pratama Sirat Muhtarudin Musthofa, Arfan Fahrizki Permata Sari, Lilis Pramana, Weldi Adi Pratama Adi Prayoga Pratama, Ridho PUJI LESTARI Purnama Edy Santosa Putra, Alvin Permana Putri Pramudita Putri, Elly Yani Medyas Raden Mohamad Herdian Bhakti Ramadani, Fajar Ratna Ermawati Reni Indrayanti Reza Ramdan Rivai Rosita Rosita Rr Riyanti, Rr Rudy Sutrisna Samliok Ndobe Saputri, Nenti Sari, Berliana Setio, Slamet Setyadi, Hario Jati Slamet Susanto Sukmadjaja, D. Sulastri Sulastri Sulistiawan, Dicky Syinwani, Billy M Taruk, Medi TRI JOKO SANTOSO Tri Muji Ermayanti Triyani Dewi Veithzal Rivai Zainal Veronica Wanniatie Widiasri, Ni Luh Putu Widiawati, Arlintia Wulandari, Dyah Retno Wulandari, Dyah Retno Zakirah Raihani Ya’la