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All Journal Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Jurnal Hortikultura Indonesia (JHI) Majalah Ilmiah Peternakan Suluh Pembangunan: Journal of Extension and Development AGRIVITA, Journal of Agricultural Science BERITA BIOLOGI Prosiding SAKTI (Seminar Ilmu Komputer dan Teknologi Informasi) Jurnal Riset dan Inovasi Peternakan Buletin Penelitian Tanaman Rempah dan Obat Wahana Peternakan Jurnal Riset dan Inovasi Peternakan (Journal of Research and Innovation of Animals) Journal La Lifesci JUSTICIA SAINS: Jurnal Ilmu Hukum SmartComp Berita Biologi

Ali Husni

Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian, Jalan Tentara Pelajar 3A, Bogor 16111

Author-ID : 283551

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Electrical & Electronics Engineering Environmental Science Immunology & microbiology Law, Crime, Criminology & Criminal Justice Social Sciences Veterinary

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Analisis Korelasi dan Regresi antara Volume Tubuh dengan Bobot Tubuh Kambing Saburai Menggunakan Persamaan Linear (Studi Kasus di Kelompok Ternak Tani Makmur II kecamatan Gisting Kabupaten Tanggamus Provinsi Lampung) Febriyanti, Leni Nur; Dakhlan, Akhmad; Husni, Ali; Qisthon, Arif
Jurnal Riset dan Inovasi Peternakan (Journal of Research and Innovation of Animals) Vol 8 No 4 (2024): Jurnal Riset dan Inovasi Peternakan: November 2024
Publisher : Department of Animal Husbandry, Faculty of Agriculture, University of Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jrip.2024.8.4.729-736

Penelitian ini bertujuan untuk mengetahui korelasi dan regresi antara bobot tubuh (BT) dengan volume tubuh (VT) yang dihitung menggunakan panjang badan (PB) dan lingkar dada (LD), pada kambing Saburai betina di Kelompok Tani Ternak II, Kecamatan Gisting, Kabupaten Tanggamus, Provinsi Lampung yang dilaksanakan pada Januari 2023. Ternak yang digunakan dalam penelitian ini merupakan kambing Saburai betina berumur 1—3 tahun sebanyak 33 ekor dan tidak bunting. Metode yang digunakan adalah survei dengan pengambilan data dilakukan secara purposive sampling dengan studi kasus. Data yang diperoleh ditabulasi menggunakan program excel. Data dianalisis dengan analisis korelasi dan regresi linear antara variabel independent (PB, LD, and VT) dan variabel dependen (BT) menggunakan program R. Hasil penelitian menunjukkan bahwa LD, PB, dan VT memiliki korelasi dengan BT kambing Saburai betina, dimana LD memiliki korelasi kuat dengan nilai 0,7586; PB memiliki korelasi yang kuat dengan nilai 0,7419; dan VT memiliki nilai korelasi yang kuat dengan nilai 0,8408. Sedangkan persamaan regresi antara lingkar dada (LD), panjang badan (PB), dan volume tubuh (VT) terhadap bobot tubuh masing-masing yaitu BT = -50,1805 + 1,1924LD; BT = -25,1642 + 0,8728PB; BT = 5,8799 + 0,001VT dengan koefisien determinasi (R²) secara berurutan yaitu 0,6378; 0,5504; dan 0,7070.

Embriogenesis dan Desikasi Embrio Somatik Jeruk Keprok Batu 55 (Citrus reticulata Blanco.) untuk Meningkatkan Frekuensi Perkecambahan Fathur Rahmi, Atika; Purwito, Agus; Husni, Ali; Dinarti, Diny
Jurnal Hortikultura Indonesia (JHI) Vol. 8 No. 2 (2017): Jurnal Hortikultura Indonesia
Publisher : Indonesian Society for Horticulture / Department of Agronomy and Horticulture

ABSTRACTIn vitro breeding technique of citrus is effective when optimum explant regeneration method is obtained. Low germination frequency and high abnormality were barrier in citrus somatic embryogenesis. This research aimed at optimizing somatic embryogenesis in Tangerine var. Batu 55. This research consisted of 3 experiments. First experiment was maturation of embryogenesis, using Completely Randomized Design (CRD) method. Modified MS+MW was used as basic media added with 500 mg L-1 malt extract (control) and addition of 3 mg L-1 BAP, and 2.5 mg L-1 ABA as treatments. Second experiment was SE (cotyledonary phage) desiccation. Factorial CRD used in two factors. First factor was poly-ethylene-glicol/PEG 8000 (0, 2.5, 5, 7.5 and 10%), while second factor was immersed periods (control, 3, 6, and 9 hours), in desiccant solution (base medium + PEG). Third experiment was studied of plantlet growth and development planlets. Based on CRD 2 factor method, the first factor was PEG concentrations from the second experiment. Second factor were active charcoal treatments (with or without), in basic media. The result showed that 2.5 mg L-1 ABA produced has highest mature somatic embryo (SE). Desiccation for 9 hours, induced the highestt germination frequencies (90.29%). The best growth of plantlets shown in previous experiments immersed desiccant PEG 2.5% for 9 hours, and cultured in basic media with 2 g L-1 of activated charcoal.Keywords: desiccant, embryogenic callus, maturation, PEG 8000, somatic embryo ABSTRAK Pemuliaan tanaman melalui teknik in vitro efektif bila metode regenerasi eksplan optimum telah diperoleh. Rendahnya frekuensi perkecambahan dan tingginya abnormalitas, menjadi kendala pada embriogenesis somatik jeruk. Penelitian terdiri atas 3 percobaan paralel, bertujuan mengoptimalkan metode embriogenesis somatik jeruk, khususnya Keprok Batu 55. Percobaan pertama pematangan kalus embriogenik menggunakan Rancangan Acak Lengkap (RAL) satu faktor, dengan perlakuan penambahan ZPT (kontrol, 3 mg L-1 BAP, dan 2.5 mg L-1 ABA) pada media dasar (MS modifikasi vitamin MW) diperkaya 500 mg L-1 ekstrak malt. Percobaan kedua desikasi embrio somatik (fase kotiledon) menggunakan RAL dua faktor. Faktor pertama konsentrasi poly-ethylene-glicol/PEG 8000 (0, 2.5, 5, 7.5 dan 10%), dan faktor kedua waktu perendaman (kontrol, 3, 6, dan 9 jam) pada larutan desikan (media dasar + PEG). Percobaan ketiga mempelajari pertumbuhan dan perkembangan planlet, menggunakan RAL dua faktor. Faktor pertama konsentrasi PEG planlet pada percobaan kedua, dan faktor kedua perbedaan media dasar (tanpa dan dengan arang aktif). Hasil percobaan menunjukkan penambahan 2.5 mg L-1 ABA menghasilkan maturasi embrio somatik terbaik. Desikasi 9 jam menghasilkan frekuensi perkecambahan 90.29%. Pertumbuhan terbaik ditunjukkan planlet yang pada percobaan sebelumnya direndam 9 jam desikan PEG 2.5%, dan dibesarkan pada media dasar dengan 2 g L-1 arang aktif.Kata kunci : desikan, embrio somatik, kalus embriogenik, PEG 8000, pematangan

Perkecambahan dan Pertumbuhan in vitro Jeruk Nipis (Citrus aurantifolia) Komang Trisna Wirakusuma; Purwito, Agus; Husni, Ali; Kosmiatin, Mia
Jurnal Hortikultura Indonesia (JHI) Vol. 14 No. 1 (2023): Jurnal Hortikultura Indonesia
Publisher : Indonesian Society for Horticulture / Department of Agronomy and Horticulture

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jhi.14.1.1-8

In vitro selection to increase the citrus resistance to Huanglongbin-HLB diseases, requires theavailability of lime in vitro shoots as a negative control, because it is one type of citrus that is verysusceptible to HLB. In vitro shoots can be obtained from seed germination in vitro with a more controlledenvironment. Shoots derived from in vitro germination can be used as controls when in vitro artificialinoculation by pathogen suspension is performed to select in vitro breeding lines. This study aims to obtainthe best concentration of gibberellins and scarification treatment for in vitro germination of lime.Germination was carried out on citrus seeds that had been sterilized on the surface of the fruit. Scarificationis done by injuring the testa of the seed. Lime seeds with and without scarification treatment were germinated on MS+VMW base media with the addition of GA3 0 mg L-1, 5 mg L-1, 10 mg L-1, and 15 mgL-1. The results showed that the optimal concentration of GA3 to induce lime seed germination was 5 mgL-1. Scarification by wounding the seed testas significantly accelerated lime germination time. Keywords: Citrus aurantifolia, in vitro selection, negative control, of GA3, scarification

Induksi Tetraploid Tunas Pucuk Jeruk Siam Simadu (Citrus nobilis Lour) Menggunakan Kolkisin secara In Vitro Yulianti, Fitri; Purwito, Agus; Husni, Ali; Dinarti, Diny
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 43 No. 1 (2015): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Seedless fruit is one of the criteria (necessary) to improve the quality of Simadu tangerine. The most effective method to obtain seedless triploid cultivars is hybridisation between tetraploid and diploid parents. Simadu tangerine is a diploid plant. Tetraploid Simadu tangerine can be obtained with doubling chromosome using colchicines.The aim of this research was to obtain tetraploid Simadu tangerine shoot which would serve as parent to produced seedless Simadu tangerine. Shoot-tips of Simadu tangerine without leaves were treated with colchicines at four different concentrations (0, 0.1, 0.2, and 0.3%) for 3 hours. The results showed that the high concentration of 0.3% reduced survival rate. The colchicine treatments reduced growth of shoot-tip of Simadu Tangerine.The leaves of colchicines treated shoots were thicker than control. Leaves from control (0% colchicine) and 0.1% colchicine treated shoots had 8.67 and 18.25 chloroplast per pair of guard cells. Compared to those of control, leaves with 0.1% colchicine had lower stomatal density, and larger stomatal size. It appeared that 0.1% colchicine treatment resulted in tetraploid Simadu Tangerine Shoot.Keywords:chloroplasts, doubling chromosomes, stomatal size, stomatal density

Radiosensitivitas dan Seleksi Mutan Putatif Jeruk Keprok Garut (Citrus reticulata L.) berdasarkan Penanda Morfologi ,, Karyanti; Purwito, Agus; Husni, Ali
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 43 No. 2 (2015): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

ABSTRACTMandarin Citrus cv. garut is a of local citrus variety with several superiority, but not competitive to imported citrus. Quality improvement have been the subject of citrus breeding programme. The objective of this research was to determine lethal dose 50 (LD50) and putative mutans regenerant selected based on morphological markers. Callus was irradiated at doses of 0, 20, 30, 40, 50, 60, 70, 80, 90 and 100 Gy and regenerated through somatic embryogenesis. The result of radiosensitivity showed that lethal dose 50 (LD50) analyzed by Curve Expert 1.4 software was 75.31 Gy. Observation on callus showed variation on morphology and weight of callus. Callus growth was not inhibited at doses 0-50 Gy but at doses 60-100 Gy callus growth was inhibited. Gamma irradiation also affected the formations of somatic embryos. After six weeks on maturation medium, the highest number of embryo somatic was produced from doses of 20 and 100 Gy and following culture on germination medium, the highest number of planlet was produced from doses of 20 and 40 Gy. Based on morphological characters, the selected regenerants had variability of 0-58%.Keywords: gamma irradiation, germination, lethal dosis 50 (LD50), maturation, somatic embryo

Transformation of Ponkan Mandarin (Citrus reticulata Blanco) by CRISPR/Cas-9-gRNA-CsCS to increase plant resistance to huanglongbing disease Dian Puji Rahayu; Purwito, Agus; Kosmiatin, Mia; Husni, Ali
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 52 No. 2 (2024): Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy)
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24831/jai.v52i2.53465

The gene that regulates callose could be modified by CRISPR/Cas9 technology. This research aimed to insert the CRISPR/Cas9-CsCS gene into Ponkan orange genome using Agrobacterium tumefaciens. The explants were soaked in a bacterial suspension for 20 minutes and incubated for 2-3 days. In vitro acceleration growth was conducted with a two-factor completely randomized design. The first factor is the type of explant with three levels (nucellar embryo, zygotic embryo, cotyledon node), and the second factor is the type of media with 6 levels (VMW, MT, MSK0, MSK1, MSK2, MSK3). The results showed of all explant types, the highest plant height average and number of leaves were obtained in a media combination of MS + Kinetin 2 mg L-1, MT, and MS + Kinetin 3 mg L-1. The highest transformation efficiency was in the nucellar embryo explant, while the highest regeneration efficiency was in the zygotic embryo explant. The highest shoot tip grafting percentage was achieved in the cotyledon node explant at 100%. In the grafting phase, the putative transformants before and after artificial bacterial inoculation showed that Ponkan 606 and Ponkan 597 had the highest plant heights, respectively. The intensity of Huanglongbing attacks after bacterial inoculation showed that three genotypes did not show HLB symptoms in the 24th week of observation, namely genotypes 598, 606, and 607. This study concluded that gene transformation in citrus plants produced three genotypes that did not show HLB symptoms. Keywords: transgenic; Agrobacterium tumefaciens; Citrus Vein Phloem Degeneration (CVPD)

INDUKSI POLIPLOIDI TANAMAN TELANG (Clitoria ternatea L.) DENGAN KOLKISIN Fransiska; Husni, Ali; Indrayanti, Reni
Berita Biologi Vol 23 No 3 (2024): Berita Biologi
Publisher : BRIN Publishing (Penerbit BRIN)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/berita_biologi.2024.2513

Tanaman telang dapat dimanfaatkan sebagai alternatif pakan ternak karena kandungan nutrisinya. Produktivitas tanaman telang dapat ditingkatkan dengan menginduksi tanaman poliploidi dengan kolkisin. Tanaman poliploidi mempunyai ukuran fenotipik yang besar daripada diploidnya yang berpengaruh terhadap produktivitas tanaman tersebut. Tujuan dari penelitian ini adalah mengetahui pengaruh jenis telang dan lama perendaman kolkisin terhadap kecambah, karakter fenotipik dan tingkat ploidi tanaman. Penelitian ini dilaksanakan di Balai Besar Pengujian Standar Instrumen Bioteknologi dan Sumber Daya Genetik Pertanian (BSIP Biogen). Penelitian ini menggunakan Rancangan Acak Lengkap dengan duafaktor yaitu jenis telang (putih dan biru) dan lama perendaman kolkisin (0, 24, dan 48 jam. Data dianalisis secara statistik dengan ANAVA pada taraf nyata 5%. Telang biru dengan perendaman kolkisin selama 24 jam menghasilkan panjang daun, panjang stomata dan jumlah kloroplas tertinggi. Telang putih yang diberikan perlakuan perendaman kolkisin selama 24 jam menghasilkan tinggi kecambah dan lebar kotiledon yang paling tinggi dan perendaman kolkisin selama 48 jam menghasilkan karakter lebar daun, panjang dan lebar mahkota bunga terbesar. Telang biru dan putih dengan perlakuan perendaman kolkisin selama 24 dan 48 jam menghasilkan tanaman miksoploid (diploid dan tetraploid).

Urgensi Reaktualisasi Undang-Undang Nomor 28 Tahun 2014 Untuk Menjawab Tantangan Perlindungan Hak Cipta Digital Habibi, Muhammad; Annisa, Ajeng Nur; Husni, Ali
JUSTICIA SAINS - Jurnal Ilmu Hukum Vol 10, No 2 (2025): JUSTICIA SAINS: Jurnal Ilmu Hukum
Publisher : Universitas Sang Bumi Ruwa Jurai

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24967/jcs.v10i2.4123

Digital transformation presents new challenges for copyright protection in Indonesia. Law No. 28 of 2014 on Copyright is considered to be insufficiently responsive to the dynamics of digital infringement, such as illegal distribution, works based on modern technology, and weaknesses in the protection system and royalty distribution on digital platforms. This article discusses the urgency of updating the Copyright Law to make it more adaptive, including expanding the scope of protection in the digital realm, integrating security technologies such as Digital Rights Management (DRM) and watermarking, strengthening the responsibility of User-Generated Content (UGC) platforms, and aligning regulations with international standards. Key challenges include the difficulty of cross-border law enforcement, low public literacy, dualism in protection systems, and the lack of transparency in collective management organizations. Proposed solutions include inter-agency collaboration, improving public literacy, utilizing work protection technologies, and reforming the royalty distribution system. With comprehensive regulatory updates and cross-sector collaboration, copyright protection in Indonesia is expected to become more effective and competitive in the digital age.

IMPLEMENTASI SISTEM AKUARIUM IKAN LOUHAN MENGGUNAKAN FUZZY LOGIC Afriliana, Ida; Husni, Ali
Smart Comp :Jurnalnya Orang Pintar Komputer Vol 11, No 3 (2022): Smart Comp: Jurnalnya Orang Pintar Komputer
Publisher : Politeknik Harapan Bersama

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30591/smartcomp.v11i3.3712

Salah satu teknologi yang saat ini sedang populer adalah teknologi yang bersifat otomatis. Sistem yang bersifat otomatis berpengaruh terhadap kehidupan manusia yang menjadikan aktivitasnya menjadi lebih efektif dan efisien salah satunya dalam pemeliharan ikan Louhan. Tujuan dari penelitian ini adalah membuat Implementasi Sistem Akuarium Ikan Louhan Menggunakan Fuzzy Logic. Logika Fuzzy merupakan metode yang dapat digunakan untuk mengatasi masalah yang mempunyai banyak jawaban dan digunakan untuk menjelaskan ketidakjelasan menurut Prof. Lofti Zadeh. Pada penelitian ini penulis menggunakan fuzzy logic metode mamdani dengan suhu dan kekeruhan sebagai input dari fuzzy dan outputnya berupa heater dan fan dan juga kondisi air. Hasil yang diperoleh dari penelitian ini adalah mengetahui suhu air di dalam akuarium ikan louhan dan mengetahui tingkat kekeruhan air akuarium, dengan hasil untuk suhu ketika 260C heater akan menyala dan fan mati, dan ketika suhu 260C â€“ 300C heater dan fan mati, ketika suhu 300C heater mati dan fan menyala. Kekeruhan mendapatkan hasil jika 650 kondisi air keruh, jika 450 â€“ 650 kondisi air cukup bersih, dan jika 450 kondisi air bersih. Dari aspek suhu dan kekeruhan tersebut dapat dimonitoring melalui website.Kata Kunci:Â Metode Fuzzy Logic, Matlab 2015, Sistem Kontrol

Biopharmaceutical potential of indonesian husk tomato (Physalis angulata L.): genetic diversity, metabolite biosynthesis, and breeding for enhanced fruit quality Shoidah, Fajriyatus; Fadillah, Muhammad Asya; Martasari, Chaireni; Nugroho, Kristianto; Wulandari, Dyah Retno; Kosmiatin, Mia; Husni, Ali; Waluyo, Budi; Budiarto, Kurniawan; Yusuf, Hadi Mokhamad
AGRIVITA Journal of Agricultural Science Vol 48, No 3 (2026): October 2026
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v48i3.5055

The Husk Tomato (Physalis angulata L.) has been valued as a medicinal plant due to its rich secondary metabolites. These compounds include physalins, which exhibit antitumor activity; withanolides, recognized as potent anti-inflammatory agents; and other components such as flavonoids, phenolic acids, alkaloids, and carotenoids. Despite its therapeutic potential, the commercial utilization of P. angulata remains limited by inconsistent fruit quality. This review summarizes current knowledge on Indonesian P. angulata germplasm, focusing on its botany, growth characteristics, genetic diversity, and metabolite profiles. Previous reviews mainly discussed botanical and pharmacological properties, whereas this review emphasizes Indonesian germplasm diversity and breeding perspectives. We critically evaluate several breeding strategies aimed at improving fruit quality, including the utilization of genetic resources, hybridization, mutation breeding, polyploidization, and in vitro techniques. Furthermore, we highlight the application of genetic engineering, particularly the overexpression of the squalene synthase (SQS) gene, suggesting that this approach could efficiently enhance the biosynthesis of squalene derivatives, such as physalins and withanolides. By evaluating the achievements and challenges of these strategies in P. angulata and other Solanaceae species, this review offers a roadmap for future research, aiming to maximize fruit quality and unlock the extensive biopharmaceutical applications of the Indonesian Husk Tomato.

Co-Authors Adhitia, Fabian Agus Joko Santoso Agus Purwito Annisa, Ajeng Nur Antika, Dea Mela Arif Qisthon Arzakiyah, Fina Baroya, Mushlihatun Beninda, Adellia Budi Waluyo Budiman, Edy Budiyanto, Ferly Rahmat Chaireni Martasari Dakhlan, Akhmad Daniatur, Arya Desyandri Desyandri Dewi, Anggi Derma Tungga Dian Puji Rahayu Dian Septinova Diny Dinarti Disa, Putra Rama edy santosa, purnama Eka Rosyida Erwanto Erwanto Fadillah, Muhammad Asya Farida Fathul Fathur Rahmi, Atika fatimah Fatimah Febriyanti, Leni Nur Fitri Yulianti Fransiska Hamdani, M Dima Iqbal Hobir, NFN Ida Afriliana Ilhamsyah, Alifudin Indonant, Hassem Muhamad Inka Aulia Karyanti ,, Karyanti Kohir, Muhammad Zahir Alwan Komang Trisna Wirakusuma KRISTIANTO NUGROHO, KRISTIANTO Kurniantha, Muhammad Aldi Kurniawan Budiarto Kusuma Adhianto Liman Liman Liman, Liman Madi Hartono Maemunah Maemunah Maliya, Mirwa Siti Medi Taruk Mia Kosmiatin Muhammad Habibi Muhammad Mirandy Pratama Sirat Muhtarudin Musthofa, Arfan Fahrizki Permata Sari, Lilis Pramana, Weldi Adi Pratama Adi Prayoga Pratama, Ridho Purnama Edy Santosa Putra, Alvin Permana Putri Pramudita Putri, Elly Yani Medyas Raden Mohamad Herdian Bhakti Ramadani, Fajar Ratna Ermawati Reni Indrayanti Reza Ramdan Rivai Rosita Rosita Rr Riyanti, Rr Rudy Sutrisna Samliok Ndobe Sari, Berliana Setio, Slamet Setyadi, Hario Jati Shoidah, Fajriyatus Slamet Susanto Sukmadjaja, D. Sulastri Sulastri Sulistiawan, Dicky TRI JOKO SANTOSO Tri Muji Ermayanti Triyani Dewi Veithzal Rivai Zainal Veronica Wanniatie Widiasri, Ni Luh Putu Widiawati, Arlintia Wulandari, Dyah Retno Wulandari, Dyah Retno Yusuf, Hadi Mokhamad Zakirah Raihani Ya’la

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