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All Journal Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Jurnal Hortikultura Indonesia (JHI) Majalah Ilmiah Peternakan Suluh Pembangunan: Journal of Extension and Development AGRIVITA, Journal of Agricultural Science BERITA BIOLOGI ANNALES BOGORIENSES Prosiding SAKTI (Seminar Ilmu Komputer dan Teknologi Informasi) Jurnal Riset dan Inovasi Peternakan Buletin Penelitian Tanaman Rempah dan Obat Wahana Peternakan Jurnal Riset dan Inovasi Peternakan (Journal of Research and Innovation of Animals) Journal La Lifesci JUSTICIA SAINS: Jurnal Ilmu Hukum SmartComp Annales Bogorienses Berita Biologi

Ali Husni

Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian, Jalan Tentara Pelajar 3A, Bogor 16111

Author-ID : 283551

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Electrical & Electronics Engineering Environmental Science Immunology & microbiology Law, Crime, Criminology & Criminal Justice Medicine & Pharmacology Social Sciences Veterinary

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PENGARUH PEMBERIAN SILASE KULIT PISANG TERHADAP PERFORMA DAN KONVERSI RANSUM PADA DOMBA EKOR TIPIS Maliya, Mirwa Siti; Adhianto, Kusuma; Liman, Liman; Husni, Ali
Jurnal Riset dan Inovasi Peternakan (Journal of Research and Innovation of Animals) Vol 9 No 3 (2025): Jurnal Riset dan Inovasi Peternakan: Agustus 2025
Publisher : Department of Animal Husbandry, Faculty of Agriculture, University of Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jrip.2025.9.3.564-573

This research aims to determine the best treatment for giving banana peel silage on body weight gain, ration consumption and ration conversion. This research was carried out in December 2023 ̶ February 2024 located at the Animal Husbandry Department, Faculty of Agriculture, Lampung University, Bandar Lampung. This research was carried out using a Randomized Block Design (RAK) consisting of 3 treatments and 5 replications, using 15 thin-tailed sheep. The treatment used is P0: 50% concentrate + 50% tebon silage; P1: 50% concentrate + 35% tebon silage + 15% banana peel silage; P2: 50% concentrate + 20% corn stalks silage + 30% banana peel silage. The data obtained were analyzed using Analysis of Variance (ANOVA). The results of the research on daily body weight gain were (P0 50,57±29,78; P1 90,19±11,03; P2 62,64±32,31) gr/day, on ration consumption of (P0 1118,18±71,06; P1 1252,47±241,82; P2 1322,82±138,50) gr/day, and at ration conversion of (P0 25,38±14,46; P1 14±2,71; P2 25,89±14,69). Providing banana peel silage in treatment P1 had the best effect on daily body weight gain and feed consumption. The P2 treatment had the best effect on feed conversion.

PENDUGAAN BOBOT TUBUH SAPI SIMPO MENGGUNAKAN UKURAN TUBUH DAN VOLUME TUBUH DI KELOMPOK TERNAK LIMOUSIN LIVESTOCK KECAMATAN PUNGGUR KABUPATEN LAMPUNG TENGAH Musthofa, Arfan Fahrizki; Dakhlan, Akhmad; Husni, Ali; Adhianto, Kusuma
Jurnal Riset dan Inovasi Peternakan (Journal of Research and Innovation of Animals) Vol 9 No 3 (2025): Jurnal Riset dan Inovasi Peternakan: Agustus 2025
Publisher : Department of Animal Husbandry, Faculty of Agriculture, University of Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jrip.2025.9.3.612-625

Penelitian ini bertujuan untuk menduga bobot tubuh sapi Simpo menggunakan ukuran-ukuran tubuh dan volume tubuh yang dihitung dari panjang badan dan lingkar dada. Penelitian ini dilaksanakan di Kelompok Ternak Limousin Livestock Kecamatan Punggur Kabupaten Lampung Tengah. Metode yang digunakan pada penelitian ini adalah survey. Teknik pengambilan sampel dilakukan dengan cara purposive sampling dengan cara mengukur dan menimbang tubuh sapi Simpo dengan kriteria sapi berjenis kelamin jantan, umur sapi 1-4 tahun, dan bobot tubuh berkisar 200-550 kg. Data yang diperoleh kemudian ditabulasi menggunakan program excel kemudian data panjang badan, lingkar dada, dan volume tubuh dianalisis korelasi dan regresinya dengan bobot tubuh sapi Simpo. Analisis korelasi dan regresi antara variable independen dan variabel dependen menggunakan program R. Hasil penelitian menunjukkan bahwa nilai korelasi antara lingkar dada, panjang badan, dan volume tubuh terhadap bobot tubuh masing-masing yaitu 0,81, 0,87, dan 0,91. Sedangkan persamaan regresi antara panjang badan (PB), lingkar dada (LD) dan volume tubuh (VT) terhadap bobot tubuh masing-masing yaitu BT = -306,8569 + 4,6133PB; BT = -453,0868 + 4,7904LD; dan BT = 68,69 + 0,0008738VT, dengan koefisien determinasi masing-masing adalah 0,66; 0,76; dan 0,82. Kesimpulan penelitian ini adalah bahwa ukuran tubuh dan volume tubuh dapat digunakan untuk menduga bobot tubuh sapi Simpo.

Analisis Korelasi dan Regresi antara Volume Tubuh dengan Bobot Tubuh Kambing Saburai Menggunakan Persamaan Linear (Studi Kasus di Kelompok Ternak Tani Makmur II kecamatan Gisting Kabupaten Tanggamus Provinsi Lampung) Febriyanti, Leni Nur; Dakhlan, Akhmad; Husni, Ali; Qisthon, Arif
Jurnal Riset dan Inovasi Peternakan (Journal of Research and Innovation of Animals) Vol 8 No 4 (2024): Jurnal Riset dan Inovasi Peternakan: November 2024
Publisher : Department of Animal Husbandry, Faculty of Agriculture, University of Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jrip.2024.8.4.729-736

Penelitian ini bertujuan untuk mengetahui korelasi dan regresi antara bobot tubuh (BT) dengan volume tubuh (VT) yang dihitung menggunakan panjang badan (PB) dan lingkar dada (LD), pada kambing Saburai betina di Kelompok Tani Ternak II, Kecamatan Gisting, Kabupaten Tanggamus, Provinsi Lampung yang dilaksanakan pada Januari 2023. Ternak yang digunakan dalam penelitian ini merupakan kambing Saburai betina berumur 1—3 tahun sebanyak 33 ekor dan tidak bunting. Metode yang digunakan adalah survei dengan pengambilan data dilakukan secara purposive sampling dengan studi kasus. Data yang diperoleh ditabulasi menggunakan program excel. Data dianalisis dengan analisis korelasi dan regresi linear antara variabel independent (PB, LD, and VT) dan variabel dependen (BT) menggunakan program R. Hasil penelitian menunjukkan bahwa LD, PB, dan VT memiliki korelasi dengan BT kambing Saburai betina, dimana LD memiliki korelasi kuat dengan nilai 0,7586; PB memiliki korelasi yang kuat dengan nilai 0,7419; dan VT memiliki nilai korelasi yang kuat dengan nilai 0,8408. Sedangkan persamaan regresi antara lingkar dada (LD), panjang badan (PB), dan volume tubuh (VT) terhadap bobot tubuh masing-masing yaitu BT = -50,1805 + 1,1924LD; BT = -25,1642 + 0,8728PB; BT = 5,8799 + 0,001VT dengan koefisien determinasi (R²) secara berurutan yaitu 0,6378; 0,5504; dan 0,7070.

Embriogenesis dan Desikasi Embrio Somatik Jeruk Keprok Batu 55 (Citrus reticulata Blanco.) untuk Meningkatkan Frekuensi Perkecambahan Fathur Rahmi, Atika; Purwito, Agus; Husni, Ali; Dinarti, Diny
Jurnal Hortikultura Indonesia (JHI) Vol. 8 No. 2 (2017): Jurnal Hortikultura Indonesia
Publisher : Indonesian Society for Horticulture / Department of Agronomy and Horticulture

ABSTRACTIn vitro breeding technique of citrus is effective when optimum explant regeneration method is obtained. Low germination frequency and high abnormality were barrier in citrus somatic embryogenesis. This research aimed at optimizing somatic embryogenesis in Tangerine var. Batu 55. This research consisted of 3 experiments. First experiment was maturation of embryogenesis, using Completely Randomized Design (CRD) method. Modified MS+MW was used as basic media added with 500 mg L-1 malt extract (control) and addition of 3 mg L-1 BAP, and 2.5 mg L-1 ABA as treatments. Second experiment was SE (cotyledonary phage) desiccation. Factorial CRD used in two factors. First factor was poly-ethylene-glicol/PEG 8000 (0, 2.5, 5, 7.5 and 10%), while second factor was immersed periods (control, 3, 6, and 9 hours), in desiccant solution (base medium + PEG). Third experiment was studied of plantlet growth and development planlets. Based on CRD 2 factor method, the first factor was PEG concentrations from the second experiment. Second factor were active charcoal treatments (with or without), in basic media. The result showed that 2.5 mg L-1 ABA produced has highest mature somatic embryo (SE). Desiccation for 9 hours, induced the highestt germination frequencies (90.29%). The best growth of plantlets shown in previous experiments immersed desiccant PEG 2.5% for 9 hours, and cultured in basic media with 2 g L-1 of activated charcoal.Keywords: desiccant, embryogenic callus, maturation, PEG 8000, somatic embryo ABSTRAK Pemuliaan tanaman melalui teknik in vitro efektif bila metode regenerasi eksplan optimum telah diperoleh. Rendahnya frekuensi perkecambahan dan tingginya abnormalitas, menjadi kendala pada embriogenesis somatik jeruk. Penelitian terdiri atas 3 percobaan paralel, bertujuan mengoptimalkan metode embriogenesis somatik jeruk, khususnya Keprok Batu 55. Percobaan pertama pematangan kalus embriogenik menggunakan Rancangan Acak Lengkap (RAL) satu faktor, dengan perlakuan penambahan ZPT (kontrol, 3 mg L-1 BAP, dan 2.5 mg L-1 ABA) pada media dasar (MS modifikasi vitamin MW) diperkaya 500 mg L-1 ekstrak malt. Percobaan kedua desikasi embrio somatik (fase kotiledon) menggunakan RAL dua faktor. Faktor pertama konsentrasi poly-ethylene-glicol/PEG 8000 (0, 2.5, 5, 7.5 dan 10%), dan faktor kedua waktu perendaman (kontrol, 3, 6, dan 9 jam) pada larutan desikan (media dasar + PEG). Percobaan ketiga mempelajari pertumbuhan dan perkembangan planlet, menggunakan RAL dua faktor. Faktor pertama konsentrasi PEG planlet pada percobaan kedua, dan faktor kedua perbedaan media dasar (tanpa dan dengan arang aktif). Hasil percobaan menunjukkan penambahan 2.5 mg L-1 ABA menghasilkan maturasi embrio somatik terbaik. Desikasi 9 jam menghasilkan frekuensi perkecambahan 90.29%. Pertumbuhan terbaik ditunjukkan planlet yang pada percobaan sebelumnya direndam 9 jam desikan PEG 2.5%, dan dibesarkan pada media dasar dengan 2 g L-1 arang aktif.Kata kunci : desikan, embrio somatik, kalus embriogenik, PEG 8000, pematangan

Perkecambahan dan Pertumbuhan in vitro Jeruk Nipis (Citrus aurantifolia) Komang Trisna Wirakusuma; Purwito, Agus; Husni, Ali; Kosmiatin, Mia
Jurnal Hortikultura Indonesia (JHI) Vol. 14 No. 1 (2023): Jurnal Hortikultura Indonesia
Publisher : Indonesian Society for Horticulture / Department of Agronomy and Horticulture

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jhi.14.1.1-8

In vitro selection to increase the citrus resistance to Huanglongbin-HLB diseases, requires theavailability of lime in vitro shoots as a negative control, because it is one type of citrus that is verysusceptible to HLB. In vitro shoots can be obtained from seed germination in vitro with a more controlledenvironment. Shoots derived from in vitro germination can be used as controls when in vitro artificialinoculation by pathogen suspension is performed to select in vitro breeding lines. This study aims to obtainthe best concentration of gibberellins and scarification treatment for in vitro germination of lime.Germination was carried out on citrus seeds that had been sterilized on the surface of the fruit. Scarificationis done by injuring the testa of the seed. Lime seeds with and without scarification treatment were germinated on MS+VMW base media with the addition of GA3 0 mg L-1, 5 mg L-1, 10 mg L-1, and 15 mgL-1. The results showed that the optimal concentration of GA3 to induce lime seed germination was 5 mgL-1. Scarification by wounding the seed testas significantly accelerated lime germination time. Keywords: Citrus aurantifolia, in vitro selection, negative control, of GA3, scarification

Induksi Tetraploid Tunas Pucuk Jeruk Siam Simadu (Citrus nobilis Lour) Menggunakan Kolkisin secara In Vitro Yulianti, Fitri; Purwito, Agus; Husni, Ali; Dinarti, Diny
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 43 No. 1 (2015): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Seedless fruit is one of the criteria (necessary) to improve the quality of Simadu tangerine. The most effective method to obtain seedless triploid cultivars is hybridisation between tetraploid and diploid parents. Simadu tangerine is a diploid plant. Tetraploid Simadu tangerine can be obtained with doubling chromosome using colchicines.The aim of this research was to obtain tetraploid Simadu tangerine shoot which would serve as parent to produced seedless Simadu tangerine. Shoot-tips of Simadu tangerine without leaves were treated with colchicines at four different concentrations (0, 0.1, 0.2, and 0.3%) for 3 hours. The results showed that the high concentration of 0.3% reduced survival rate. The colchicine treatments reduced growth of shoot-tip of Simadu Tangerine.The leaves of colchicines treated shoots were thicker than control. Leaves from control (0% colchicine) and 0.1% colchicine treated shoots had 8.67 and 18.25 chloroplast per pair of guard cells. Compared to those of control, leaves with 0.1% colchicine had lower stomatal density, and larger stomatal size. It appeared that 0.1% colchicine treatment resulted in tetraploid Simadu Tangerine Shoot.Keywords:chloroplasts, doubling chromosomes, stomatal size, stomatal density

Radiosensitivitas dan Seleksi Mutan Putatif Jeruk Keprok Garut (Citrus reticulata L.) berdasarkan Penanda Morfologi ,, Karyanti; Purwito, Agus; Husni, Ali
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 43 No. 2 (2015): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

ABSTRACTMandarin Citrus cv. garut is a of local citrus variety with several superiority, but not competitive to imported citrus. Quality improvement have been the subject of citrus breeding programme. The objective of this research was to determine lethal dose 50 (LD50) and putative mutans regenerant selected based on morphological markers. Callus was irradiated at doses of 0, 20, 30, 40, 50, 60, 70, 80, 90 and 100 Gy and regenerated through somatic embryogenesis. The result of radiosensitivity showed that lethal dose 50 (LD50) analyzed by Curve Expert 1.4 software was 75.31 Gy. Observation on callus showed variation on morphology and weight of callus. Callus growth was not inhibited at doses 0-50 Gy but at doses 60-100 Gy callus growth was inhibited. Gamma irradiation also affected the formations of somatic embryos. After six weeks on maturation medium, the highest number of embryo somatic was produced from doses of 20 and 100 Gy and following culture on germination medium, the highest number of planlet was produced from doses of 20 and 40 Gy. Based on morphological characters, the selected regenerants had variability of 0-58%.Keywords: gamma irradiation, germination, lethal dosis 50 (LD50), maturation, somatic embryo

Transformation of Ponkan Mandarin (Citrus reticulata Blanco) by CRISPR/Cas-9-gRNA-CsCS to increase plant resistance to huanglongbing disease Dian Puji Rahayu; Purwito, Agus; Kosmiatin, Mia; Husni, Ali
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 52 No. 2 (2024): Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy)
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24831/jai.v52i2.53465

The gene that regulates callose could be modified by CRISPR/Cas9 technology. This research aimed to insert the CRISPR/Cas9-CsCS gene into Ponkan orange genome using Agrobacterium tumefaciens. The explants were soaked in a bacterial suspension for 20 minutes and incubated for 2-3 days. In vitro acceleration growth was conducted with a two-factor completely randomized design. The first factor is the type of explant with three levels (nucellar embryo, zygotic embryo, cotyledon node), and the second factor is the type of media with 6 levels (VMW, MT, MSK0, MSK1, MSK2, MSK3). The results showed of all explant types, the highest plant height average and number of leaves were obtained in a media combination of MS + Kinetin 2 mg L-1, MT, and MS + Kinetin 3 mg L-1. The highest transformation efficiency was in the nucellar embryo explant, while the highest regeneration efficiency was in the zygotic embryo explant. The highest shoot tip grafting percentage was achieved in the cotyledon node explant at 100%. In the grafting phase, the putative transformants before and after artificial bacterial inoculation showed that Ponkan 606 and Ponkan 597 had the highest plant heights, respectively. The intensity of Huanglongbing attacks after bacterial inoculation showed that three genotypes did not show HLB symptoms in the 24th week of observation, namely genotypes 598, 606, and 607. This study concluded that gene transformation in citrus plants produced three genotypes that did not show HLB symptoms. Keywords: transgenic; Agrobacterium tumefaciens; Citrus Vein Phloem Degeneration (CVPD)

In Vitro Induction of Tetraploid Pummelo ’Nambangan’ (Citrus maxima (Burm.) Merr.) by Colchicine Treatment Using Germinated Seed, Shoot Tip and Cotyledonary Node As Explants Wulandari, Dyah Retno; Purwito, Agus; Susanto, Slamet; Husni, Ali; Ermayanti, Tri Muji
Annales Bogorienses Vol. 19 No. 1 (2015): Annales Bogorienses
Publisher : BRIN

Show Abstract | Download Original | Original Source | Check in Google Scholar

Tetraploid citrus are important for interploidal hybridization to create triploid seedless citrus. Colchicine is the most commonly used as antimitotic agent to induce polyploid plants. Tetraploid induction by colchicine in Pummelo ‘Nambangan’ was conducted in vitro using different types of explants. The aim of this research was to induce tetraploid pummelo ‘Nambangan’ by colchicine treatment using germinated seed, shoot tip and cotyledonary node as explants. Tetraploid shoot induction was conducted by soaking germinated seeds, shoot tips and cotyledonary nodes in 0.1 % colchicine for 1, 3 and 5 hours. Regenerant shoots were grown on MS medium and their growth was observed after four weeks in culture. Ploidy level was determined using flow cytometry analysis. Stomata density, length and width of stomatal guard cell were also recorded. The results showed that shoot elongation was inhibited by colchicine treatment. Soaking of shoot tip explants in 0.1 % colchicine for 1 hour resulted in 66.66 % of putative tetraploid shoots. Compared to diploid shoots, tetraploids had lower stomata density but bigger in guard cell size.

Characterization of Zygotic and Nucellar Embryo of Six Indonesian Mango Cultivars Using Molecular Markers Fatimah, Fatimah; Husni, Ali; Kosmiatin, Mia; Karsinah, Karsinah; Baroya, Mushlihatun
Annales Bogorienses Vol. 20 No. 2 (2016): Annales Bogorienses
Publisher : BRIN

Show Abstract | Download Original | Original Source | Check in Google Scholar

One of difficulties in mangoes hybridization is polyembryonic seeds. This phenomenon reduces the chance of recovering true hybrid seedlings. The identification of the zygotic embryo is difficult and the possible approach is by using molecular marker. The objective of this study was to evaluate the utilization of two different marker systems (dominant markers and SSR) to characterize the occurrence of zygotic or nucellar embryo in polyembryony mango cultivars Garifta Merah, Lalijiwo, Manalagi, Madu, Saigon Kuning, and Saigon Merah. The type of embryo was evaluated by comparing the exhibiting amplification patterns, if different from the mother plant considered as zygotic and identified as nucellar if they exhibited the same banding pattern as the mother plant. From both of this marker systems out of the 16 SSR and 16 evaluated dominant primers, nine primers of each systems amplified the largest number of allele and sharply defined band. Dendogram analysis showed that the evaluated dominant markers could distinguish the zygotic and nucellar embryo clearly compare to evaluated SSR primers. The number of zygotic embryos derived from SSR was 64% and dominant markers were 47%. Based on zygotic and nucellar size, number and position, indicating no relationship between the type of embryo and embryo size, number and the position.

Co-Authors Adhitia, Fabian Agus Joko Santoso Agus Purwito Annisa, Ajeng Nur Antika, Dea Mela Arif Qisthon Arzakiyah, Fina Baroya, Mushlihatun Beninda, Adellia Budi Waluyo Budiman, Edy Budiyanto, Ferly Rahmat Chaireni Martasari Dakhlan, Akhmad Daniatur, Arya Desyandri Desyandri Dewi, Anggi Derma Tungga Dian Puji Rahayu Dian Septinova Diny Dinarti Disa, Putra Rama edy santosa, purnama Eka Rosyida Erwanto Erwanto Fadillah, Muhammad Asya Farida Fathul Fathur Rahmi, Atika fatimah Fatimah Febriyanti, Leni Nur Fitri Yulianti Fransiska Hamdani, M Dima Iqbal Hobir, NFN Ida Afriliana Ilhamsyah, Alifudin Indonant, Hassem Muhamad Inka Aulia Karsinah - Karyanti ,, Karyanti Kohir, Muhammad Zahir Alwan Komang Trisna Wirakusuma KRISTIANTO NUGROHO, KRISTIANTO Kurniantha, Muhammad Aldi Kurniawan Budiarto Kusuma Adhianto Liman Liman Liman, Liman Madi Hartono Maemunah Maemunah Maliya, Mirwa Siti Medi Taruk Mia Kosmiatin Muhammad Habibi Muhammad Mirandy Pratama Sirat Muhtarudin Musthofa, Arfan Fahrizki Permata Sari, Lilis Pramana, Weldi Adi Pratama Adi Prayoga Pratama, Ridho Purnama Edy Santosa Putra, Alvin Permana Putri Pramudita Putri, Elly Yani Medyas Raden Mohamad Herdian Bhakti Ramadani, Fajar Ratna Ermawati Reni Indrayanti Reza Ramdan Rivai Rosita Rosita Rr Riyanti, Rr Rudy Sutrisna Samliok Ndobe Sari, Berliana Setio, Slamet Setyadi, Hario Jati Shoidah, Fajriyatus Slamet Susanto Sukmadjaja, D. Sulastri Sulastri Sulistiawan, Dicky TRI JOKO SANTOSO Tri Muji Ermayanti Triyani Dewi Veithzal Rivai Zainal Veronica Wanniatie Widiasri, Ni Luh Putu Widiawati, Arlintia Wulandari, Dyah Retno Wulandari, Dyah Retno Yusuf, Hadi Mokhamad Zakirah Raihani Ya’la

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