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All Journal HAYATI Journal of Biosciences Saintek Perikanan : Indonesian Journal of Fisheries Science and Technology BIOTROPIA - The Southeast Asian Journal of Tropical Biology The Journal of Experimental Life Sciences (JELS) Jurnal Kelautan : Indonesian Journal of Marine Science and Technology Jurnal EECCIS Journal of Innovation and Applied Technology Research Journal of Life Science Journal of Environmental Engineering and Sustainable Technology Indonesian Green Technology Journal Jurnal Pembangunan dan Alam Lestari REKAYASA JFMR (Journal of Fisheries and Marine Research) Omni-Akuatika Jurnal Penelitian Pendidikan IPA (JPPIPA) Jurnal Riset Akuakultur Samakia: Jurnal Ilmu Perikanan PengabdianMu: Jurnal Ilmiah Pengabdian kepada Masyarakat Journal of Aquaculture and Fish Health Jurnal Akuakultura Universitas Teuku Umar JATI EMAS (Jurnal Aplikasi Teknik dan Pengabdian Masyarakat) Jurnal Pengabdian pada Masyarakat Ilmu Pengetahuan dan Teknologi Terintegrasi Depik Jurnal Ilmu-Ilmu Perairan, Pesisir dan Perikanan Journal of Fisheries & Marine

Maftuch Maftuch

Faculty Of Fisheries And Marine Science, Brawijaya University, Malang, Indonesia

Author-ID : 2841312

Agriculture, Biological Sciences & Forestry Humanities Automotive Engineering Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Control & Systems Engineering Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Education Electrical & Electronics Engineering Energy Engineering Environmental Science Immunology & microbiology Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Mechanical Engineering Medicine & Pharmacology Neuroscience Physics Public Health Social Sciences Veterinary Other

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The Effect of Hermetia illucens Larvae on the Hematology of Tilapia (Oreochromis niloticus) Infected Edwardsiella tarda Tirani, Elisabeth; Maftuch, Maftuch; Fadjar, Mohammad; Awaluddin, Muhammad
Journal of Aquaculture and Fish Health Vol. 13 No. 1 (2024): JAFH Vol. 13 No. 1 February 2024
Publisher : Department of Aquaculture

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jafh.v13i1.36213

Non-specific defense is the main defense in fish. One of the natural ingredients as a source immunostimulant isH. illucens larvae with a protein content of up to 30%. H. illucens larvae contains alkaloids, tannins, terpenoidsand saponins as immunostimulating agents. Immunostimulants are biological compounds that can boost theimmune system body. The purpose of this study is to find out the effect of H. illucens larvae in increasingimmunity non specific in tilapia (O. niloticus) which infected with E. tarda. In giving H. illucens larvae feed adlibitum with concentrations of A(30%), B(40%), C(50%), K(0%). In this research method, a completelyrandomized design (CRD) was used with 5 treatments and 3 replications in which each aquarium contained 10tilapia. Parameters tested are leukocytes, differential leukocytes, erythrocytes and hemoglobin. The resultsshowed that intake of 50% H. illucens larvae on tilapia could increase non-specific immunity, such as totalleukocytes, total erythrocytes, differential leukocytes, hemoglobin

GROWTH AND SURVIVAL RATE OF DOMESTICATED JIELABU FISH (Betta dennisyongi Tan, 2013) REARED WITH DIFFERENT STOCKING DENSITIES Saputra, Fazril; Zulfadhli, Zulfadhli; Nasution, Muhammad Arif; Syarif, Ahmad Fahrul; Maftuch, Maftuch; Abdan, Mu'amar; Utami, Diah Ayu Satyari; Sofian, Sofian
Jurnal Riset Akuakultur Vol 20, No 3 (2025): September (2025)
Publisher : Politeknik Kelautan dan Perikanan Jembrana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/jra.20.3.2025.307-317

Betta dennisyongi, locally known as Jielabu fish, is an endemic ornamental species originating from southwest Nanggroe Aceh Darussalam, Indonesia. The fish currently faces increasing exploitation and habitat degradation which raise conservation concerns and emphasize the need for domestication efforts. This study aimed to determine an appropriate stocking density for the early culture of B. dennisyongi. The research was conducted from October to December 2023 using a completely randomized design with four stocking densities (1, 2, 3, and 4 fish L⁻¹) with three replications. Juvenile fish collected from the wild were acclimated and reared for 60 days in 10-L aquaria. Biological parameters measured included survival, specific growth rate, length gain, weight gain, feed conversion ratio, and feed efficiency, while water quality was monitored periodically. Stocking density significantly affected survival rate and growth (p < 0.05) of B. dennisyongi, with the lowest density (1 fish L⁻¹) producing the most favorable performance, while densities of 3–4 fish L⁻¹ resulted in reduced outcomes. Feed conversion ratio and feed efficiency did not differ significantly among treatments (p > 0.05). All measured water quality parameters remained within acceptable ranges for Betta fish culture. Overall, the study indicates that lower stocking densities support better biological responses, and that 1 fish L⁻¹ is the most suitable for cultivation, while 2 fish L⁻¹ may serve as an acceptable alternative for practical application. These findings provide a foundation for developing effective domestication protocols and support conservation-oriented aquaculture of this vulnerable endemic species. Betta dennisyongi, yang dikenal secara lokal sebagai ikan Jielabu, merupakan spesies ikan hias endemik dari wilayah barat daya Nanggroe Aceh Darussalam, Indonesia. Peningkatan pemanfaatan dan degradasi habitatnya telah menimbulkan kekhawatiran dalam kegiatan konservasi dan menekankan perlunya upaya domestikasi. Penelitian ini bertujuan untuk menentukan padat tebar yang sesuai untuk pemeliharaan awal B. dennisyongi. Penelitian dilakukan pada bulan Oktober hingga Desember 2023 menggunakan rancangan acak lengkap dengan empat padat tebar (1, 2, 3, dan 4 ekor L⁻¹) masing-masing dengan tiga ulangan. Benih ikan yang dikumpulkan dari alam diaklimatisasi dan dipelihara selama 60 hari dalam akuarium berkapasitas 10 L. Parameter biologis yang diukur meliputi kelangsungan hidup, laju pertumbuhan spesifik, pertambahan panjang, pertambahan bobot, rasio konversi pakan, dan efisiensi pakan, sedangkan kualitas air dipantau secara berkala. Padat tebar berpengaruh nyata terhadap kelangsungan hidup dan pertumbuhan (p < 0.05) B. dennisyongi, di mana padat tebar terendah (1 ekor L⁻¹) memberikan performa terbaik, sedangkan padat tebar 3–4 ekor L⁻¹ menghasilkan performa yang lebih rendah. Rasio konversi pakan dan efisiensi pakan tidak berbeda nyata antarperlakuan (p > 0.05). Seluruh parameter kualitas air berada dalam kisaran yang sesuai untuk budidaya Betta fish. Secara keseluruhan, penelitian ini menunjukkan bahwa padat tebar rendah mendukung respons biologis yang lebih baik, dan 1 ekor L⁻¹ merupakan padat tebar yang paling sesuai, sementara 2 ekor L⁻¹ masih dapat diaplikasikan. Temuan ini menjadi dasar bagi pengembangan protokol domestikasi yang efektif dan mendukung budidaya yang berorientasi pada konservasi bagi spesies endemik rentan ini.

THE RANGE EXPANSION OF Parachromis managuensis (GÜNTHER, 1867) (PERCIFORMES, 4 CICHLIDAE) IN JAVA, INDONESIA Hasan, Veryl; Faqih, Abdul Rahem; Maftuch, Maftuch
BIOTROPIA Vol. 29 No. 1 (2022): BIOTROPIA Vol. 29 No. 1 April 2022
Publisher : SEAMEO BIOTROP

The Jaguar cichlid, Parachromis managuensis (Günther, 1867), is native to Central America, with introductions reported from West Java and Central Java Provinces of Indonesia. On 7-8 January 2019, sixteen specimens of P. managuensis were collected from Karangkates, the largest hydropower reservoir in East Java Province of Indonesia. A description of the morphological characters of specimens is provided.

Kajian Penggunaan Ciprofloxacin terhadap Histologi Insang dan Hati Ikan Botia (Botia macracanthus, Bleeker) yang Diinfeksi Bakteri Aeromonas hydrophila Sukarni, Sukarni; Maftuch, Maftuch; Nursyam, Happy
The Journal of Experimental Life Science Vol. 2 No. 1 (2012)
Publisher : Graduate School, Universitas Brawijaya

Aeromonas hydrophila mampu menyebabkan terjadinya infeksi dan kematian ikan botia dalam waktu yang relatif singkat pada konsentrasi yang tinggi, terbukti dengan konsentrasi 108 selml-1 menyebabkan 50% ikan uji mati. Hasil pengamatan histopatologi pada ikan terinfeksi menunjukkan ginjal yang mengalami degenerasi hyaline, munculnyavakuola yang disebabkan lisis pada glomerulus dan kemudian hancur, nekrosis pada glomerulus, terdapat banyak koloni bakteri A.hydrophila didalam ginjal, serta terjadi infiltrasi limfosit. Sel hati tidak terlihat jelas karena tertutup infiltrasi limfosit dan koloni bakteri A.hydrophila yang sangat banyak, nekrosis yang menyebabkan vakuola, terdapat infeksi sekunder bakteri yang belum teridentifikasi, serta terjadi cloudy swelling. Pada bagian insang, nekrosis lamela primer menyebabkan munculnya vakuola, kongesti pada lamela primer dan edema pada lamela sekunder, infiltrasi limfosit, poliferasi sel/fusi lamela serta hyperplasia pada lamela primer. Sementara itu pada ikan botia yang terinfeksi A.hydrophila tetapi kemudian diobati, ginjal mengalami perbaikan glomerulus dan kapsula bowman sehingga terlihat jelas, tubuli berbentuk seperti donat dengan corak titik dan garis, tidak ada lagi nekrosis dan vakuola, tidak ditemukan lagi koloni bakteri, terjadi perbaikan hyaline yang mengalami degenerasi, jaringan hematopoietic (pembentuk sel-seldarah merah) terlihat jelas dengan inti yang bulat. Pada bagian hati, tidak ditemukan lagi koloni bakteri A.hydrophila dan bakteri sekunder lainnya sehingga hepatosit terlihat jelas dengan bentuk polyhedral dengan inti 1-2, jumlah eritrosit terlihat normal, karena tidak ada lagi infiltrasi limfosit. Akan tetapi masih terdapat penyumbatan pada vena centralis yang dipenuhi oleh eritrosit. Pada insang tidak ada lagi nekrosis dan vakuola pada lamela primer sehingga jaringan terlihat solid, namun ditemukan infeksi sekunder parasit Monogenea sp. Tidak ada lagi infiltrasi limfosit, lapisan epithelium terlihat 1-2 lapis, tidak ada lagi edema dan fusi lamela sehingga lamela tampak jelas dengan ukuran panjang yang bervariasi. Kata Kunci : histopatologi, Aeromonas hydrophila, Ikan Botia

Kajian Penggunaan Ciprofloxacin terhadap Hematologi Ikan Botia (Botia macracanthus, Bleeker) yang Diinfeksi Bakteri Aeromonas hydrophila Maftuch, Maftuch; Nursyam, Happy; Sukarni, Sukarni
The Journal of Experimental Life Science Vol. 2 No. 2 (2012)
Publisher : Graduate School, Universitas Brawijaya

Uji hematologi sangat bermanfaat sebagai alat diagnostik didalam menetapkan status kesehatan ikan. Salah satu aspek dari infeksi adalah terjadinya perubahan gambaran darah. Pemeriksaan darah dapat digunakan sebagai indikator keparahan suatu penyakit tertentu. Beberapa parameter yang dapat memperlihatkan perubahan patologi pada darah meliputi jumlah leukosit, deferensial leukosit, eritrosit. Dari hasil penelitian diketahui jumlah rata-rata sel darah merah dalam darah ikan botia sehat pada masing-masing perlakuan K-, K+, A, B, C, D berturut-turut adalah 2.470.000, 2.500.000, 2.560.000, 2.350.000, 2.440.000, dan 2.430.000. Sementara itu pada ikan botia yang terinfeksi bakteri A.hydrophila diketahui jumlah rata-rata eritrosit pada masing-masing perlakuan K-, K+, A, B, C, dan D berturut-turut adalah 2.420.0000, 1.770.000, 1.780.000, 1.890.000, 1.780.000, dan 1.870.000. jumlah rata-rata eritrosit ikan botia yang terinfeksi bakteri A.hydrophila, dan kemudian diobati dengan ciprofloxacin pada masing-masing perlakuan K-, K+,A, B, C, dan D berturut-turut adalah 2.420.000, 1.630.000, 2.090.000, 2.280.000, 2.500.000, dah 2.540.000. Sementara jumlah rata-rata sel darah putih dalam darah ikan botia yang sehat pada masing-masing perlakuan K-, K+, A, B, C, D berturut-turut adalah 97.000, 90.000, 88.000, 87.000, 93.000 dan 88.000. Sementara itu jumlah rata-rata sel darah putih pada ikan botia yang terinfeksi bakteri A.hydrophila, pada masing-masing perlakuan K-, K+, A, B, C, D berturut-turut adalah 97.000, 174.000, 174.000, 179.000, 185.000 dan 185.000. Sedangkan dari hasil penelitian terhadap ikan botia yang terinfeksi A.hydrophila dan kemudian diobati dengan ciprofloxacin diperoleh hasil perhitungan rata-rata leukosit pada masing-masing perlakuan berturut-turut adalah sebagai berikut 980.000, 189.000, 125.000, 137.000, 133.000, dan 136.000. Kata Kunci : Aeromonas hydrophila, eritrosit, hemotologi, ikan botia, leukosit

Dried Skeletonema costatum in Feed Formulation for the Growth of Vaname Shrimp (Litopenaeus vannamei) Lestari, Dewi Putri; Ekawati, Arning W.; Maftuch, Maftuch
The Journal of Experimental Life Science Vol. 4 No. 2 (2014)
Publisher : Graduate School, Universitas Brawijaya

The aim of this study was to determine the effect and the best dosage used by the Skeletonema costatum in feed formulation on the growth of Vaname shrimp. This experiment used Completely Randomized Design (CRD) with four treatments and three replications. Treatment was given by using dried S. costatum in feed formula (iso protein 37% and iso energy 3.6 kkal/g feed). Feed treatment was the substitution of fish meal protein A = 0%, B = 2.5%, C = 5% and D = 7.5% with dried S. costatum protein. The observed parameters include Survival Rate (SR), Survival Growth Rate (SGR), Feed Efficiency Ratio (FER), and Protein Efficiency Ratio (PER). The results showed that the use of dried S. costatum in feeding are effective in increasing the specific growth rate, feed efficiency ratio and the ratio of protein efficiency than controls. The best dosage in feeding formula is ranged from 4.39% to 4.75%.Keywords: feeding formula, Skeletonema costatum, Vaname Shrimp

Analysis of Genotype and Phylogenetic of Koi Herpesvirus (KHV) on Carp (Cyprinus carpio) in Lombok Island of West Nusa Tenggara Province Based on Thymidine Kinase Sequence Baihani, Amira; Yanuhar, Uun; Maftuch, Maftuch
The Journal of Experimental Life Science Vol. 8 No. 1 (2018)
Publisher : Graduate School, Universitas Brawijaya

Koi herpesvirus (KHV) is a virus that usually infects carp and koi causing mass mortality, as well as economic and social loss. The results of varied test are often found in its development. Allegedly, there are mutation or genetic variation of KHV in Indonesia. Based on these problems, this research aims to understand the genetic variation and kindship of KHV isolate in the carp (Cyprinus carpio) in Lombok Island of West Nusa Tenggara Province based on thymidine kinase sequence. Water quality of fish pond was checked. KHV clinical symptom was checked visually in each fish sample, then sacrifices the organ (gill) for PCR analysis and DNA sequencing. The research results show that clinical symptoms that appear are pale fish gill and bleeding on the surface of the body. The temperature in the research site shows an optimum range for KHV development. The results electrophoresis of carp suspected with KHV shows five positives out of a total six samples. Genetically, KHV isolates from Lombok Island of West Nusa Tenggara Province is identical with the GenBank isolate code KHV-J/U/I and Indo_0C05WNT2.Keywords: Carp, Koi herpesvirus, Phylogenetic, Thymidine kinase.

Expression of Hsp70 and β-actin Genes as The Immune Response against Viral Nervous Necrosis that Infected Asian Seabass (Lates calcalifer) Wahyudi, Yusuf Arif; Yanuhar, Uun; Maftuch, Maftuch
The Journal of Experimental Life Science Vol. 8 No. 3 (2018)
Publisher : Graduate School, Universitas Brawijaya

The viral nervous necrosis (VNN) is betanodavirus of the family Nodaviridae with acute infection and associated with high levels mortality up to 100% of numerous larval-stage marine and freshwater fish species. The danger signals of viral infection that are capable of activating APCs (Antigen Presenting Cells), furthermore produced molecules protein receptors such as interferon and heat shock proteins. Stimulating interferon type I (IFN I) induce several antiviral molecules, further binding the actin cytoskeleton to reach the site of infection. This study demonstrated a correlation between increased levels of Hsp70 (heat shock proteins) and actin filamentous (β-actin) within invasion wild-type isolate of ssRNA VNN from Asian Seabass (L. calcalifer) juvenile. Furthermore, expression of Hsp70 and actin as an indicator or biomarker of stressed states in fish. The reverse transcriptase polymerase chain reaction (RT-PCR) method used to finding expression Hsp70 and β-actin. Whereas nested RT-PCR used for VNN genes. The up-regulation of Hsp70 was observed on the brain tissue higher than eye tissue of sample positive infected VNN. Whereas different expression of β-actin, β-actin receptor expression tends to be stable in the organs of the brain and eye, both invaded VNN or normal, while on the eye, the ratio slightly increased based on the intensity of the band. The research shows that altered expression of heat shock protein 70 and beta-actin gene receptors in the target organs that response to invasion VNN at Asian seabass (L. calcalifer) juvenile. Alteration expression of heat shock protein 70 and beta-actin can be used as an indicator or biomarker of stress cells, especially from VNN invasion at fish. Keywords: Asian seabass, β-actin, Hsp70, Immune response, Viral nervous necrosis.

Potential of Dayak Onion (Eleutherine palmifolia (L) Merr) Extract As Antibacterial Against Pseudomonas fluorescens Fransira, Immaria; Yanuhar, Uun; Maftuch, Maftuch
The Journal of Experimental Life Science Vol. 9 No. 2 (2019)
Publisher : Graduate School, Universitas Brawijaya

Dayak onions (E. palmifolia (L) Merr) is a herb used as medicine and known to have antibacterial activity. This research aimed to evaluate antibacterial activity of Dayak onion (Eleutherine palmifolia (L) Merr) extract against fish pathogen Pseudomonas fluorescens. The method used in this study was extraction by maceration, UV-Vis spectrophotometry analysis on the extract and antibacterial activity test with Minimum Inhibitory Concentration (MIC) as well as disc diffusion test. The results of this study showed that the yield value was 8.87%. Based on UV-Vis analysis, it confirmed that extract of Dayak onion (Eleutherine palmifolia (L) Merr) contains predominantly flavonoids and phenols with its derivatives, that have antibacterial activity. The results of MIC and disc diffusion test showed potential antibacterial activity against P. fluorescens, as observed from the value of absorbance and inhibition zones that formed. Keywords: Antibacterial, Disc diffusion, Eleutherine palmifolia (L) Merr, MIC, Pseudomonas fluorescens.

Analiysis of Phytoplankton Diversity on the Productivity of Vannamei Shrimp (Litopenaeus vannamei) Intensive Pond, Jatisari Village, Banyuwangi Juliyanto, Nabilla Anggi; Maftuch, Maftuch; Masithah, Endang Dewi
The Journal of Experimental Life Science Vol. 11 No. 2 (2021)
Publisher : Graduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jels.2021.011.02.01

Phytoplankton can be used to estimate the potential for vannamei shrimp production. It can be used as a provider of nutrient sources and has an important role in improving water quality. This study aims to analyze the phytoplankton community structure on the productivity of intensive vannamei shrimp ponds. The research was conducted in Jatisari Village, Banyuwangi, East Java, Indonesia. Carried out parameters were the calculation of density, phytoplankton diversity index, culture performance (SR, FCR, ADG), and water quality (temperature, water transparency, salinity, pH, DO, NO2, NO3, PO4, NH4). The results showed that there were six classes and 33 genera from both ponds, Chlorophyceae (10 genera), Bacillariophyceae (8 genera), Cyanophyceae (9 genera), Dinophyceae (3 genera), Euglonophyceae (1 genus), Criptophyceae (1 genus). The index value of the two ponds shows moderate diversity, H 'pond 1 is 1.76, and pond 2 is 2.02. The two plots' cultivation performance was SR 92% and 80%, FCR 1.08 and 1.13, ADG 0.31 g.day-1 and 0.35 g.day-1, respectively. The physical and chemical parameters of the research showed a good enough value for the life of vannamei shrimp and phytoplankton.Keywords: Cultivation performance, Phytoplankton, Vannamei shrimp.

Co-Authors A'yunin, Qurrota Abdul Rahem Faqih Achmad Noercholis Ade Yamindago Agus Budiarto Ahmad Fahrul Syarif Andhang Sebastian Andriyanto, Septyan Annisa Fitri Anggreini Ari Wahjudi Arief Prajitno Arif Hoetoro Arning W. Ekawati, Arning W. Arning Wilujeng Ekawati Asep A. Prihanto Asep Awaludin Prihanto Ash Shufi, Abdillah Hanan Astuti, Retno Tri Asus Maizar Suryanto H Baihani, Amira Bramantiyo Satriyo Wicaksosno D. Ardiansyah Daniar Kusumawati Desy Setyoningrum Dewi Putri Lestari, Dewi Putri DIAH AYU SATYARI UTAMI Dwi R. Fatmawati E. Sanoesi Ellana Sanoesi Endang Dewi Masithah Fadjar, Mohammad Farikhah, Reghita Dwi Febby Hadi Setyawan Febi Kesid Haryo Wibisono Febi Nadhila Nurin Friyuanita, Friyuanita Gede Angga Krishna Fariestha H. Suprastyani Happy Nursyam Hariati, Anik Martinah Heny Suprastyani Heny Suprastyani Heny Suprastyani, Heny Herlina, Cucun Hermawan Meidy Kurnianto Ihsan Sanggar Rahman Ika K. Nisyak Immaria Fransira Ismaningdyah Kurniawati Juanda, Eki Juliyanto, Nabilla Anggi Karimah Karimah Lila Gardenia M. Aziz Muslim Marsoedi Marsoedi Mauludia, Farida Mochamad Awaludin Adam Moh. Awaludin Adam MOHAMAD FADJAR Mohammad Bisri Mohammad Holil Lulloh Muhammad Arif Nasution Muhammad Arif Nasution Muhammad Awaluddin, Muhammad Muhammad Dailami, Muhammad Muhammad Musa Muhammad Musa Muhammad Nursid Mu’amar Abdan N. Farida N. Habibah Nafiqoh, Nunak Novita, Hessy Nuranti Anarkhis Nurin, Febi Nadhila Pratama, Vian Dedi Prihanto, Asep A. Putra, Gede A. A. K. Putra, Mirsa F. R. Rinaldi Redi Bintarto, Redi Rudianto Raharjo Runi Asmaranto Samuki, Khairul Sanoesi, Ellana Sebastian, Andhang Setyawan, Fahreza Okta Soelistyoadi, Rachmat Noer Sofian, Sofian Sri Andayani Sri Andayani Sri Rahayu Sri Widyarti Sugiarto S Suhermanto, Achmad Sukarni Sukarni Supriatin, Febriyani Eka Susanti, Marlinda Elvina Syarifah Hikmah J. S Teguh Dwi Widodo Tirani, Elisabeth Vendrita Damayanti Putri Veryl Hasan W., Denny Widya Wahyudi, Yusuf Arif Yahya Yahya Yanuhar, Uun Yenny Risjani Yuni Kilawati Zulfadhli Zulfadhli Zulfadhli Zulfadhli

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