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All Journal HAYATI Journal of Biosciences Saintek Perikanan : Indonesian Journal of Fisheries Science and Technology BIOTROPIA - The Southeast Asian Journal of Tropical Biology The Journal of Experimental Life Sciences (JELS) Jurnal Kelautan : Indonesian Journal of Marine Science and Technology Jurnal EECCIS Journal of Innovation and Applied Technology Research Journal of Life Science Journal of Environmental Engineering and Sustainable Technology The Indonesian Green Technology Journal Jurnal Pembangunan dan Alam Lestari REKAYASA JFMR (Journal of Fisheries and Marine Research) Omni-Akuatika Jurnal Penelitian Pendidikan IPA (JPPIPA) Samakia: Jurnal Ilmu Perikanan PengabdianMu: Jurnal Ilmiah Pengabdian kepada Masyarakat Jurnal Akuakultura Universitas Teuku Umar JATI EMAS (Jurnal Aplikasi Teknik dan Pengabdian Masyarakat) Jurnal Pengabdian pada Masyarakat Ilmu Pengetahuan dan Teknologi Terintegrasi Depik Jurnal Ilmu-Ilmu Perairan, Pesisir dan Perikanan Depik Jurnal Ilmu-Ilmu Perairan, Pesisir, dan Perikanan Journal of Fisheries & Marine
Maftuch Maftuch
Faculty Of Fisheries And Marine Science, Brawijaya University, Malang, Indonesia
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Pengembangan “Desa Wisata Kampung Koi” Latukan sebagai Upaya Penguatan BUMDesa melalui Pengembangan Kelompok Pembudidaya dan BUMDesa Latukan, Kabupaten Lamongan: Empowerment of Latukan "Koi Village" as an Effort to Strengthen BUMDesa through the Development of Fish Farmers and BUMDesa Latukan Village, The Regency of Lamongan Maftuch, Maftuch; Setyawan, Fahreza Okta; Astuti, Retno Tri; Farikhah, Reghita Dwi; Ash Shufi, Abdillah Hanan
PengabdianMu: Jurnal Ilmiah Pengabdian kepada Masyarakat Vol. 9 No. 5 (2024): PengabdianMu: Jurnal Ilmiah Pengabdian kepada Masyarakat
Publisher : Institute for Research and Community Services Universitas Muhammadiyah Palangkaraya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33084/pengabdianmu.v9i5.4362

Abstract

Latukan Village is one of the villages in Lamongan Regency with Independent Village status in the 2020 Building Village Index (IDM). Independent Village has the highest status in the IDM assessment scale published by the Ministry of Villages. Through the  Empowered Village Program, Latukan Village seeks to build a village icon by constructing the Latukan “Koi Village”. However, the complete design concept regarding the development of Latukan Village as a  Kampung Koi Tourism Village has not yet been formed optimally. Therefore, it is essential to prepare strategic development steps, which include elements of Koi Fish Cultivation, Post-harvest Management, Transportation, Marketing Management, the establishment of recreational and educational parks, as well as human resource development, in this case, the Koi Fish Cultivator Group and the Harvest Management Group. The solution is to create a development design for the Kampung Koi Tourism Village as part of the BUM Desa business unit. For this reason, the Brawijaya University Community Service team designed an activity to build a stronger and more sustainable Latukan Village Icon. Service activities consist of identifying the status of the problem and planning solutions, forming a koi fish cultivator group and making portable spawning ponds, strengthening the role of BUMDesa, village government, and cultivators through focus group discussions and comparative studies, and koi fish cultivation and breeding training. All activities have been conducted and compiled in scientific publications and mass media. In its implementation, this activity involved five students concentrating on fisheries cultivation and fishery product technology.
Uji Viabilitas Kandidat Vaksin Bakteri Aeromonas Hydrophila Menggunakan Metode Formalin-Killed dengan Konsentrasi Berbeda: Viability Assay of Aeromonas Hydrophila as Candidate Vaccine Using Formalin-Killed Method at Different Concentration Herlina, Cucun; Maftuch, Maftuch; Fadjar, Mohamad
JFMR (Journal of Fisheries and Marine Research) Vol. 9 No. 1 (2025): JFMR on March
Publisher : Faculty of Fisheries and Marine Science, Brawijaya University, Malang, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jfmr.2025.009.01.7

Abstract

Salah satu upaya pencegahan yang dapat dilakukan untuk mengatasi penyakit pada ikan yaitu menumbuhkan kekebalan seperti vaksinasi karena kekebalan tubuh yang meningkat pada ikan dapat mencegah infeksi penyakit. upaya telah dilakukan untuk mengembangkan program imunisasi yang efektif dengan menggunakan vaksin yang dibuat dari sel utuh bakteri yang diinaktivasi menggunakan formalin dan/atau pemanasan Tujuan penelitian ini untuk mengetahui hasil uji viabilitas dan hasil uji adhesi sel epitel usus menggunakan vaksin inaktif formalin dengan konsentrasi berbeda. Metode dalam penelitian ini adalah metode eksperimen dengan rancangan percobaan menggunakan rancangan acak lengkap (RAL) terdiri dari 3 perlakuan (A=2%, B=3%, dan C=4%) dan kontrol dengan 3 kali ulangan. Hasil penelitian diperoleh hasil kandidat vaksin inaktif bakteri A.hydrophila sebanyak 9 ml/tabung falcon. Infeksi bakteri A. hydrophila dengan kepadatan 109 cfu/ml sebanyak 0,1 ml dengan masa pemeliharaan 1 minggu ditandai terjadi pengerasan di bagian perut dan terdapat kerusakan pada sirip punggung pada hari ketujuh. Hasil uji viabilitas menunjukkan tidak terdapat pertumbuhan koloni bakteri A. hydrophila dibandingkan dengan kontrol. Hasil uji adhesi sel epitel usus pada kontrol dan perlakuan A, B, C diperoleh rata-rata 10.3, 0.73, 0.43, dan 0.23 bakteri/10 sel epitel. Analisis uji ANOVA diperoleh nilai uji F Hitung > F Tabel, artinya perlakuan memberikan pengaruh sangat berbeda signifikan antar semua perlakuan. Hasil analisa regresi diperoleh (R2) sebesar 0.66 bahwa hubungan keterkaitan antara vaksin inaktif konsentrasi formalin berbeda dengan jumlah bakteri A. hydrophila yang menempel pada sel epitel sebesar 66%.   One preventive effort to tackle diseases in fish is by boosting immunity, such as through vaccination, as increased immunity in fish can prevent disease infections. Efforts have been made to develop effective immunization programs using vaccines made from whole bacterial cells inactivated using formalin and/or heat. The objective of this study was to determine the results of viability tests and intestinal epithelial cell adhesion tests using formalin-inactivated vaccines with different concentrations. The method used in this study was an experimental approach with a completely randomized design (CRD) consisting of three treatments (A=2%, B=3%, and C=4%) and a control, each with three replications. The study results showed that the candidate inactivated vaccine of A. hydrophila bacteria was obtained at 9 ml/falcon tube. A. hydrophila bacterial infection with a density of 10⁹ CFU/ml at 0.1 ml and a maintenance period of one week was characterized by abdominal hardening and dorsal fin damage on the seventh day. The viability test results showed no bacterial colony growth of A. hydrophila compared to the control. The intestinal epithelial cell adhesion test results for the control and treatments A, B, and C showed average bacterial adhesion of 10.3, 0.73, 0.43, and 0.23 bacteria/10 epithelial cells, respectively. ANOVA test analysis showed that the calculated F-value was greater than the table F-value, indicating a highly significant effect among all treatments. Regression analysis resulted in an R² value of 0.66, suggesting that 66% of the variation in the adhesion of A. hydrophila bacteria to epithelial cells was associated with different formalin concentrations in the inactivated vaccine.
Molecular Detection, Histopathology, and Scanning Electron Microscopy of Myxobolus koi Infecting Cyprinus carpio Koi Soelistyoadi, Rachmat Noer; Yanuhar, Uun; Maftuch, Maftuch
The Journal of Experimental Life Science Vol. 9 No. 3 (2019)
Publisher : Graduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1153.168 KB) | DOI: 10.21776/ub.jels.2019.009.03.01

Abstract

Myxobolus koi is a type of parasite that infects many freshwater fish through myxospores, resulting in a disease called myxobolosis. This research aimed is to investigate the clinical symptoms that occur in the gills of infected Koi carp (C. carpio). The utilized methods in this study are molecular detection, histopathology, and Scanning Electron Microscopy (SEM). Koi carp samples that are suspected to be infected with Myxobolus koi show several clinical signs, such as swollen and pale-colored gills and gill covers (opercula) that do not close completely, as well as white spots that appear on the edge of the lamella. The results of this study showed that through histological observations, there were changes characterized by hyperplasia of the primary lamella cartilage that envelops parasitic cysts and the encapsulation process that surrounds the cysts by gill cartilage in infected fish;SEM showed oval and elongated spores of a size of ± 12 µm (800x). TheDNA template from gill tissues in clinically infected and uninfected fish was examined byPCR testing with primers ERB 1 (Forward) and ERB 10 (Reverse); the results of electrophoresis in infected fish were detected at 2000 bp. Keywords: Cyprinus carpio, Histopathology, Molecular Detection, Myxobolus koi, Parasite, SEM.
Phytochemical Screening and Antibacterial Activity of Kratom Leaf (Mitragyna speciosa Korth.) Against Aeromonas hydrophilla Juanda, Eki; Andayani, Sri; Maftuch, Maftuch
The Journal of Experimental Life Science Vol. 9 No. 3 (2019)
Publisher : Graduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1030.866 KB) | DOI: 10.21776/ub.jels.2019.009.03.02

Abstract

Kratom (Mitragyna speciosa) is an indigenous tropical herbal plant to the Northern Malay Peninsula and Thailand. Empirically kratom leaves have several properties as herbal medicines. Currently, the treatment of diseases caused by bacteria that are resistant to antibiotics requires new compounds that have high potential. The material studied was kratom leaf extract. The solvent used for extraction is methanol. Phytochemical screening carried out includes the examination of alkaloids, flavonoids, steroids/terpenoids, phenols, tannins, and saponins. The test bacteria used was Aeromonas hydrophilla. The medium used is Nutrient Agar (NA). NaCl 0.9% as a bacterial suspension. Testing the antibacterial activity carried out with 10 (ten) variations of concentration namely 3%, 6%, 9%, 12%, 15%, 18%, 21%, 24%, 27% and 30 %. Fresh kratom leaf is collected and then wet sorted. The extraction process was using the method of maceration. This study aims to analyze the antibacterial activity of kratom leaf, measure the secondary compounds of kratom leaf extract, and measure the best concentration to kill Aeromonas hydrophilla. The extraction process obtained blackish-brown extract with 62.27 grams of extract (31.14%). Secondary metabolites of kratom leaf extract show positive results of alkaloid, saponins, tannins, phenolics, steroids, and triterpenoids. Antibacterial activity against Aeromonas hydrophila which is characterized by a kill zone around the paper disc with the best concentration that shows a wide killing zone was 24% of the extract.Keywords: Antibacterial, Extract, Kratom, Mitragynine, Phytochemical Screening.
In silico and In vitro Antibacterial Activity of Centella asiatica Leaves Bioactive Compounds Against Aquaculture Pathogenic Bacteria Andriyanto, Septyan; Maftuch, Maftuch; Andayani, Sri; Nafiqoh, Nunak; Gardenia, Lila; Novita, Hessy; Nursid, Muhammad
Jurnal Ilmiah Perikanan dan Kelautan Vol. 17 No. 3 (2025): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.v17i3.72072

Abstract

Graphical Abstract     Highlight Research The GC-MS analysis of the asiatica leaves extracts identified 53 bioactive compounds. The crude extracts of asiatica showed antibacterial efficacy against fish pathogenic bacteria. 13-Hexyloxacyclotridec-10-en-2-one has the potential to be an inhibitor of DNA gyrase. Bioactive compounds derived from C. asiatica leaves extracts show potential as antibacterial agents.     Abstract Antimicrobial agents are crucial for managing bacterial infections in fish cultures. Centella asiatica is a medicinal plant recognised for its diverse bioactive compounds with important antibacterial properties. The present study aimed to investigate the antibacterial activity of C. asiatica leaves bioactive compounds on fish pathogenic bacteria using an in vitro and in silico approach. The maceration method was used to extract bioactive compounds from C. asiatica leaves and was identified using Gas Chromatography-Mass Spectrometry (GC-MS). In vitro analysis of antibacterial activity was evaluated using the minimum inhibitory concentration method. While in silico molecular docking is applied alongside assessing Lipinski's rules of five, as well as absorption, distribution, metabolism, excretion, and toxicity properties. The result of the GC-MS examination of the C. asiatica leaf extracts identified 53 bioactive compounds. In vitro studies showed antibacterial efficacy of leaf extracts against fish pathogenic bacteria (Streptococcus agalactiae, Bacillus subtilis, and Staphylococcus aureus) with minimum inhibitory concentration values of 12,5 mg/ml. In silico molecular docking analysis showed that several bioactive compounds have the potential to be DNA gyrase inhibitors. Compound 13-Hexyloxacyclotridec-10-en-2-one has the highest inhibition with binding energy of −7,4 Kcal/mol compared to ciprofloxacin as drug standard with a binding energy value −7,3 Kcal/mol. The following compound is gamma.-Muurolene (−6,7 Kcal/mol), Copaene (−6,6 Kcal/mol) and Humulene (−6,6 Kcal/mol). These results suggest that bioactive compounds of C. asiatica leaves extracts hold promise as potential antibacterial agents for treating fish pathogenic bacteria infections.
Effect of Extracellular Crude Protein (ECP) on expression IL-1, IL-6, IFN- in nile tilapia (Oreochromis niloticus) Herlina, Cucun; Fadjar, Mohammad; Maftuch, Maftuch; Yanuhar, Uun
Depik Jurnal Ilmu Ilmu Perairan, Pesisir, dan Perikanan Vol 14, No 3 (2025): SEPTEMBER 2025
Publisher : Faculty of Marine and Fisheries, Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13170/depik.14.3.43338

Abstract

Modern strategies incorporate preventive measures such as extracellular products, as soluble proteins with antigenic properties, as well as generating a broad immune response. The aim of research is to determine and analyze the effect of the extracellular product of E. tarda on enhancing the immunity of Nile tilapia (O. niloticus). The research design with treatment durations of d0, 3rd, 6th, 9th day, and control (A, B, C, D, K). ECP vaccination was injected intraperitoneally at a dose of 0.1 ml/fish. The parameters analyzed are the expression of IL-1, IL-6, and IFN-, phagocytic activity, leukocyte differential, total white blood cells, and relative percent survival. The results of phagocytosis activity were highest in treatment C at 84.18% 1.81. The highest leukocyte differential in treatment C was lymphocytes at 56% 2.00, monocytes at 28% 2.00, and 16% 0.00, with a total leukocyte count of 29 x 10^3 cells 2.64, and RPS values ranging from 83.33% to 100% over 9 days of post-vaccination maintenance. Then, post-vaccination, there was an increase in the expression levels of the cytokine genes IL-1 and IL-6, but there was no increase in the expression levels of the IFN- gene compared to the control in Nile tilapia. (O. niloticus).Keywords:ECPIL-1IL-6IFN-Immune Response
Effect of Extracellular Crude Protein (ECP) on expression IL-1, IL-6, IFN- in nile tilapia (Oreochromis niloticus) Herlina, Cucun; Fadjar, Mohammad; Maftuch, Maftuch; Yanuhar, Uun
Depik Jurnal Ilmu Ilmu Perairan, Pesisir, dan Perikanan Vol 14, No 3 (2025): SEPTEMBER 2025
Publisher : Faculty of Marine and Fisheries, Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13170/depik.14.3.43338

Abstract

Modern strategies incorporate preventive measures such as extracellular products, as soluble proteins with antigenic properties, as well as generating a broad immune response. The aim of research is to determine and analyze the effect of the extracellular product of E. tarda on enhancing the immunity of Nile tilapia (O. niloticus). The research design with treatment durations of d0, 3rd, 6th, 9th day, and control (A, B, C, D, K). ECP vaccination was injected intraperitoneally at a dose of 0.1 ml/fish. The parameters analyzed are the expression of IL-1, IL-6, and IFN-, phagocytic activity, leukocyte differential, total white blood cells, and relative percent survival. The results of phagocytosis activity were highest in treatment C at 84.18% 1.81. The highest leukocyte differential in treatment C was lymphocytes at 56% 2.00, monocytes at 28% 2.00, and 16% 0.00, with a total leukocyte count of 29 x 10^3 cells 2.64, and RPS values ranging from 83.33% to 100% over 9 days of post-vaccination maintenance. Then, post-vaccination, there was an increase in the expression levels of the cytokine genes IL-1 and IL-6, but there was no increase in the expression levels of the IFN- gene compared to the control in Nile tilapia. (O. niloticus).Keywords:ECPIL-1IL-6IFN-Immune Response
Co-Authors A'yunin, Qurrota Abdul Rahem Faqih Achmad Noercholis Ade Yamindago Agus Budiarto Ahmad Fahrul Syarif Amira Baihani Andhang Sebastian Andriyanto, Septyan Annisa Fitri Anggreini Ari Wahjudi Arief Prajitno Arif Hoetoro Arning Wilujeng Ekawati Asep A. Prihanto Ash Shufi, Abdillah Hanan Astuti, Retno Tri Asus Maizar Suryanto H Bramantiyo Satriyo Wicaksosno Cucun Herlina D. Ardiansyah Daniar Kusumawati Desy Setyoningrum Dwi R. Fatmawati E. Sanoesi Ellana Sanoesi Endang Dewi Masithah Fadjar, Mohammad Farikhah, Reghita Dwi Febby Hadi Setyawan Febi Kesid Haryo Wibisono Febi Nadhila Nurin Friyuanita, Friyuanita Gede Angga Krishna Fariestha H. Suprastyani Happy Nursyam Hariati, Anik Martinah Heny Suprastyani Heny Suprastyani Heny Suprastyani, Heny Herlina, Cucun Hermawan Meidy Kurnianto Ihsan Sanggar Rahman Ika K. Nisyak Immaria Fransira Ismaningdyah Kurniawati Juanda, Eki Karimah Karimah Lila Gardenia M. Aziz Muslim Marsoedi Marsoedi Mauludia, Farida Mochamad Awaludin Adam Moh. Awaludin Adam MOHAMAD FADJAR Mohammad Bisri Mohammad Holil Lulloh Muhammad Arif Nasution Muhammad Arif Nasution Muhammad Dailami, Muhammad Muhammad Musa Muhammad Musa Muhammad Nursid N. Farida N. Habibah Nabilla Anggi Juliyanto Nafiqoh, Nunak Novita, Hessy Nuranti Anarkhis Nurin, Febi Nadhila Pratama, Vian Dedi Prihanto, Asep A. Putra, Gede A. A. K. Putra, Mirsa F. R. Rinaldi Redi Bintarto, Redi Rudianto Raharjo Runi Asmaranto Samuki, Khairul Sanoesi, Ellana Sebastian, Andhang Setyawan, Fahreza Okta Soelistyoadi, Rachmat Noer Sri Andayani Sri Andayani Sri Rahayu Sri Widyarti Sugiarto S Suhermanto, Achmad Sukarni Sukarni Supriatin, Febriyani Eka Syarifah Hikmah J. S Teguh Dwi Widodo Vendrita Damayanti Putri Veryl Hasan W., Denny Widya Yahya Yahya Yanuhar, Uun Yenny Risjani Yuni Kilawati Yusuf Arif Wahyudi Zulfadhli Zulfadhli Zulfadhli Zulfadhli