Article Per Year (5 Year)
p-Index From 2021 - 2026
4.405
P-Index
This Author published in this journals
All Journal Jurnal Sain Veteriner Agrotrop : Journal on Agriculture Science International Journal of Biosciences and Biotechnology Jurnal Veteriner Indonesia Medicus Veterinus BIOTROPIA - The Southeast Asian Journal of Tropical Biology agriTECH Journal of Tropical Biodiversity and Biotechnology Jurnal Biologi Tropis Jurnal Medik Veteriner Biosaintifika: Journal of Biology & Biology Education Buletin Veteriner Udayana
I Ketut Suada
Laboratorium Kesehatan Masyarakat Veteriner, Fakultas Kedokteran Hewan, Universitas Udayana
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science Immunology & microbiology Medicine & Pharmacology Public Health Veterinary

Published : 69 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 69 Documents
Search

 3   4   5   6   7 
ANTIFUNGAL ACTIVITY OF SIAM CITRUS (Citrus nobilis L.) ESSENTIAL OIL AGAINTS Lasiodiplodia theobromae THE PATHOGEN OF BLENDOK DISEASE Pranata, Kadek Dwipayana; Wirawan, I Gede Putu; Wibawa, I Putu Agus Hendra; Suada, I Ketut; Wijaya, I Nyoman; Phabiola, Trisna Agung
International Journal of Biosciences and Biotechnology Vol 11 No 2 (2024): International Journal of Biosciences and Biotechnology
Publisher : Central Laboratory for Genetic Resource and Molecular Biology, Faculty of Agriculture, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/IJBB.2024.v11.i02.p08

Abstract

Siam citrus (C. nobilis L.) is a type of citrus that is most widely cultivated in Bangli Regency. The main disease that attacks citrus plants is blendok caused by the fungus L. theobromae. Based on research siam citrus peel contains essensial oils that have potential as antifungals. The aim of this study was to study chemical content of essensial oil from siam citrus peel and its activity as an antifungal againts L. theobromae. Essensial oil was destilled using hydro steam distillition and analyzed by means of GC-MS. Antifungal activiy testing was carried out using the agar-well diffusion method with concentrations of 1%, 10%, 25%, 50%, 75%, and 100%. Dithane M45 6 g/l and DMSO 10% were used as positive and negative controls. The results showed that the siam citrus peel from Kintamani, Bangli contains essensial oils with the main chemical components, i.e. D-limonene (57.26%), Beta-pinene (9.09%), and Beta-myrcene (4.03%) which has uses as an antifungal, additive, antitumor, asthma and allergy reliever, repellent, anti-inflammatory, antioxidant, anticancer, and antibacterial. Essential oil concentration of 25 to 100% can inhibit the growth of L. theobromae. The largest diameter of inhibition is shown at a concentration of 100% and the smallest diameter of inhibition is shown at a concentration of 25%. Keywords: C. nobilis L., essensial oils, GC-MS, L. Theobromae, antifungal
Adaptation of African Swine Virus in Non-Swine Cell Lines: A Preliminary Study for Vaccine Candidate Tenaya, I Wayan Masa; Agustina, Kadek Karang; Suada, I Ketut; Apsari, Ida Ayu Pasti; Sari, Tri Komala; Handayani, Ni Made; Widayantari, Anak Agung Ayu Sauca Sunia; Suardana, Anak Agung Komang; Sumarya, I Made; Arsana, I Nyoman; Sudiartawan, I Putu; Wahyudi, I Wayan; Juliasih, Ni Ketut Ayu; Sudaryati, Ni Luh Gede; Damriyasa, I Made
Jurnal Medik Veteriner Vol. 8 No. 1 (2025): April
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol8.iss1.2025.114-123

Abstract

African Swine Fever (ASF) is among the most detrimental infectious viral diseases in pigs causing approximately 100% mortality. The disease was first reported about 83 years ago in Africa before spreading to Europe in 1957 and Asia in 2010. An adequate vaccine generally containing live attenuated virus isolates prepared in swine macrophages to control the disease is currently unavailable. Therefore, this study aimed to use murine neuroblastoma (N2a) cells, non-swine cell lines, to adapt African swine fever virus (ASFV) isolates for vaccine preparation. ASFV isolate called BL21 obtained from Bali and East Nusa Tenggara was previously propagated in swine macrophages. However, virus was currently adapted in the N2a cells to avoid unwanted issues associated with using swine macrophages, including microbial contamination, as well as technically laborious and ethical issues. The adapted BL21 was re-confirmed with quantitative polymerase chain reaction (q-PCR) and tested in vivo to examine the pathogenicity properties. The results showed that BL21 produced consistently and specifically positive q-PCR, killing experimental pigs with typical gross pathological changes of ASF. BL21 at a 10-3/mL dilution adapted in N2a cells showed similar antigenic properties causing the death of nearly 50% N2a cells in vitro and terminating all in vivo experimental pigs. In conclusion, the BL21 isolate reported in this study could be used as a vaccine candidate after more attenuation and particularly to determine a lethal dose of 50% (LD50) for future investigations.
Morphological Identification of Balinese Caulerpa Seaweeds Dewi, Ni Kadek Emi Sintha; Wirawan, I Gede Putu; Suada, I Ketut
Jurnal Biologi Tropis Vol. 24 No. 2b (2024): Special Issue
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v24i2b.7858

Abstract

Bulung boni and bulung anggur are species of Caulerpa seaweeds commonly found along the coastline of Serangan Island, Bali. Although these species have been widely utilized in various ways, formal species identification of these two macroalgae in Bali had not been conducted prior to this study. This research aims to characterize the morphological structures of bulung boni and bulung anggur through detailed morphological examination. Identification keys and previous studies were used to assist in the morphological identification process. The findings revealed that bulung boni shares morphological characteristics with Caulerpa cylindracea, while bulung anggur is morphologically comparable to Caulerpa macrophysa. These results provide valuable insights into the species composition of seaweeds in the region and contribute to a better understanding of local biodiversity.
DNA Barcoding Based on matK Gene and Phytochemistry Analyses of Local Balinese Kayu Tangi ( Lagerstroemia sp.) Suada, I Ketut; Wirawan, I Gede Putu; Ningsih, Gusti Ketut Asti Dea; Wijaya, I Nyoman; Munthe, Lambok Jenny Sagita; Gayatri, Anak Agung Sagung Intan; Sasadara, Maria Malida Vernandes; Parwata, I Made Oka
Biosaintifika: Journal of Biology & Biology Education Vol. 16 No. 3 (2024): December 2024
Publisher : Universitas Negeri Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v16i3.9265

Abstract

Kayu tangi (Lagerstroemia sp.) is one of the medicinal plants that is often used traditionally by Balinese people for treating insomnia, diabetes, and dysentery. This study aims to determine the phylogenetic trees and the active compounds in Lagerstroemia tomentosa extract and its antioxidant activities. The methods used in molecular identification are DNA isolation, PCR, and DNA sequencing. The DNA sample was BLASTed to see its homology with the sequences in GenBank. These sequences were then used for phylogenetic tree analysis. The phytochemical analysis was done using the GC-MS method and the antioxidant activity test used the DPPH (1,1-diphenyl-2-picrylhydrazyl) after being reacted with the tested extract.  The results of molecular identification showed that the samples used were closely related to Lagerstroemia tomentosa species (MW044208.1). The antioxidant activity test results showed an IC50 of 2.1 ugml-1 which is included in the very strong category. The phytochemical test results showed that the most dominant compounds contained in the plant stem bark were furfural (AUC 8.54%), beta-sitosterol (AUC 6.00%), and gamma-sitosterol (AUC 3.81%). Based on PubChem data, these compounds have antioxidant and antimicrobial properties as well as insecticide and herbicide. In conclusion, kayu tangi is very close to  Lagerstroemia tomentosa and it’s potentially used as a medicine with strong antioxidant activities.  
TOTAL ESCHERICHIA COLI IN BROILER FECES AT VARIOUS AGES IN BADUNG REGENCY Krisis Mariani Buulolo; I Ketut Suada; I Putu Sampurna
Buletin Veteriner Udayana Bul. Vet. Udayana. December 2024 Vol. 16 No. 6
Publisher : Fakultas Kedokteran Hewan Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2024.v16.i06.p02

Abstract

Broiler chickens are quality meat-producing chickens and are known to the public with various advantages, feces are a food process accompanied by urine and undigested food residues. The purpose of this study is to determine the total number of escherichia coliw bacteria in the feces of broiler chickens of various ages in Badung Regency. This study aims complete random design consisting of 3 location factors, namely Petang District, Abiansemal District, Mengwi District. The broiler chicken feces sample taken is 2-3 grams in each farm. In this study, the culture method of escheriachia coli bacteria uses the spread method on Methene Blue agar eosin media.The sample is characterized by metallic green colonies and black dots in the middle of the colony. The analysis of the data into the Y log before being analyzed to see the number of differences in the number of E. coli bacteria is carried out by variety analysis, if there is a real difference (P<0.05) then it is continued with the Duncan Multiple Distance test. The total research results of Escherichia coli bacteria are 8,973 in the evening of 1-21 days and 8,640 in the evening of 22-35 days and 8,640 in Mengwi District and 8,640 in 1-21 days and 8,640 in 22-35 days and 8,536 in Abiansemal District at the age of 1-21 days and 8,536 in 22-35 days. It can be concluded that the Total Escherichia coli Bacteria in broiler Chicken Feces in Petang District, Mengwi District, Abiansemal District has a very real effect (P<0.05). Further research is needed on humid environmental conditions with different types of cages in broiler chickens.
TOTAL PLATE COUNTS OF BACTERIA IN THE FECES OF STARTER AND FINISHER AGE BROILERS IN BADUNG REGENCY Matilde Fatima Correia; I Ketut Suada; Tjokorda Sari Nindhia
Buletin Veteriner Udayana Bul. Vet. Udayana. February 2025 Vol. 17 No. 1
Publisher : Fakultas Kedokteran Hewan Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2025.v17.i01.p02

Abstract

Broiler chickens are a superior breed resulting from crossbreeding different chicken breeds with high productivity, especially in meat production. Chicken feces are the final product of the digestion process of feed and water consumed by livestock, in both liquid and solid forms. This study aims to determine the total plate count (TPC) of bacteria present in the feces of broiler chickens at the starter and finisher stages in Badung Regency. This research is an observational study using purposive stratified random sampling with a quantitative analysis approach. A total of 3 grams of broiler chicken feces were collected from each farm in Mengwi, Abiansemal, and Petang Districts. The total plate count of bacteria was determined using the culture method on Nutrient Agar media. The identified bacterial colonies had characteristics such as a clear white color, round shape, and glossy appearance. Data analysis was performed using variance analysis (ANOVA), and if significant differences were found (P<0.05), Duncan’s Multiple Range Test was conducted. Before analysis, the TPC data were transformed into log Y. The results showed that the TPC (log Y) in Mengwi District was 8.0608 CFU/g at 1-21 days of age and 7.6132 CFU/g at 22-35 days of age. In Abiansemal District, the values were 8.0928 CFU/g at 1-21 days and 7.7941 CFU/g at 22-35 days, while in Petang District, the values were 8.1476 CFU/g at 1-21 days and 7.9565 CFU/g at 22-35 days. Overall, the highest TPC was found in Petang District, followed by Abiansemal and Mengwi. It can be concluded that there is a significant difference (P<0.05) in the total plate count of bacteria in broiler chicken feces across the three districts based on the age groups of 1-21 days and 22-35 days. Further research is needed on broiler farm waste management following Indonesian National Standards (SNI) .
OCCUPATIONAL HEALTH AND SAFETY ANALYSIS IN THE CATTLE SLAUGHTERING PROCESS AT THE SLAUGHTERHOUSE IN DENPASAR CITY Joanita Auxilia de Sa Pereira; Kadek Karang Agustina; I Ketut Suada
Buletin Veteriner Udayana Bul. Vet. Udayana. December 2025 Vol. 17 No. 6
Publisher : Fakultas Kedokteran Hewan Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2025.v17.i06.p03

Abstract

The cattle slaughtering process at the Slaughterhouse (RPH) involves various occupational health and safety (OHS) hazards that require effective management. This study aims to identify potential OHS hazards and assess their risk levels at each stage of the cattle slaughtering process at the Denpasar City RPH. The research method used an observational approach with the Hazard Identification and Risk Assessment (HIRA) technique to analyze physical, biological, ergonomic, and psychosocial hazards. The results show that several work stages have a high-risk level (score ≥10), particularly during the slaughtering stage (physical risk score 10, biological score 12, ergonomic score 12) and the use of the restraining pen (physical risk score 16). Dominant hazards include exposure to zoonotic bacteria, injuries from sharp tools and animals, and musculoskeletal disorders due to non-ergonomic working postures. Psychosocial factors such as work stress also contributed significantly, with risk scores reaching 12 in several stages. Based on these findings, this study recommends implementing comprehensive mitigation measures, including: (1) improved use of standardized personal protective equipment (PPE), (2) regular OHS training on animal handling and tool use, (3) redesign of work facilities to be more ergonomic, (4) implementation of an ISO 45001-based OHS management system, and (5) a mental health program for workers. These recommendations are expected to reduce work accident rates and increase productivity at the Denpasar City RPH, while also serving as a reference for developing OHS policies in the livestock sector.
EFFECTIVENESS OF INDIGENOUS ENDOMYCORRHIZAL BIOFERTILIZER PROTOTYPE ON ORGANIC SALAK (Salacca zalacca) PLANTATIONS AND ITS EFFECT ON NUTRIENT AND CARBOHYDRATE CONTENT OF LEAVES Rai, I Nyoman; Suada, I Ketut; Wiraatmaja, I Wayan; Astiari, Ni Komang Alit
BIOTROPIA Vol. 28 No. 3 (2021): BIOTROPIA Vol. 28 No. 3 December 2021
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11598/btb.2021.28.3.1333

Abstract

Organically cultivated salak (Salacca zalacca) on dry land has limited production in Bali. Typically, fertilization is carried out using leaf litter or other organic fertilizers because soil fertility is low for salak plantations. The present study analyzed the effectiveness of an indigenous endomycorrhizal biofertilizer on the nutrient and total carbohydrate content of salak leaves and fruits. The study used a randomized block design with nine replicates. The treatment consisted of three levels, i.e., (1) fertilization with leaf litter only, as practiced by farmers and used as the control (C); (2) fertilization with an indigenous endomycorrhizal biofertilizer prototype (P); and (3) combined fertilization with leaf litter and an indigenous endomycorrhizal biofertilizer prototype (PM). Spores of indigenous endomycorrhizae from salak plantations were used to prepare the biofertilizer prototype. The results showed that the P and PM treatments provided beneficial results, such as: (1) significantly increasing chlorophyll content, relative water content of leaves, and the number and weight of fruits per tree; (2) improving fruit quality by increasing sweetness and weight per fruit; and (3) having a positive effect on water uptake and nutrient absorption, as indicated by high N and P contents in leaf tissue and high carbohydrate content in leaves.
Bioactive Compounds and Antibacterial Activity of Red Beetroot (Beta vulgaris L.) Ethyl Acetate Extraction Pranatayana, Ida Bagus Gde; Tampubolon, Immanuel Anthonio Caesebo; Wirawan, I Gede Putu; Wijaya, I Nyoman; Suada, I Ketut; Khalimi, Khamdan; Yudha, I Kadek Wisma
agriTECH Vol 46, No 1 (2026)
Publisher : Faculty of Agricultural Technology, Universitas Gadjah Mada, Yogyakarta, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/agritech.105476

Abstract

Red beetroot has been reported to possess various medicinal properties and antimicrobial activity. Therefore, this study aims to determine the compounds contained in the ethyl acetate extract of red beetroot pulp and evaluate its anti Escherichia coli effect. The extract was obtained by soaking the fruit in ethyl acetate, and then assessed for its components using GC-MS analysis. Antibacterial activity was tested using a disc diffusion assay. The results showed that the ethyl acetate extract of red beetroot pulp contained a total of 20 major compounds. These included ester of 1,3-benzenedicarboxylic acid and bis(2-ethylhexyl), 1,4-benzenedicarboxylic acid, and n-hexadecanoic acid. In the antibacterial test, the sample was highly effective against E. coli (IC 50 was 0,783 mg/mL). In addition, the diameter of the growth inhibition zone increased along with the extract concentration. These results suggest that the ethyl acetate extract of red beetroot pulp offers a natural antibacterial substitute to its synthetic equivalents.
Co-Authors Agnes - Tasia Agung Denlira Sitepu AGUSTINA BORU MANIK Ahmat Fansidar Aletha Yuliana Mandala, Aletha Yuliana Alhamdani, Yusuf Riska Alvionita Alexther Lingga Anak Agung Komang Suardana Anastasia Cornelia Anastasya, Sindika Anggara Fajri Prasafitra ANITA KAROLINA SIMAMORA Apriyani, Ester Muki Arhiono, Haru Nira Putra Bakar, Nor Kartini Abu Cesarina Pascalia dos Santos Alegria Chandra Immanuel Saragih Christine Regina Fenita Wenno, Christine Regina Fenita Cita, I Putu Giri Wahyu Eka Dayana, Anak Agung Putri Isadela Dewi, Ni Kadek Emi Sintha Dimas Indra Dwi Purnama Dwi Yoga Sutrisna Dyah Ayu Widiasih Eggy Hidta Lusandika ELIYA KUSUMAWATI Elly Hariati Br Purba, Elly Hariati Br Emerensia Patryconsitha Aman Enjelina, Ni Desak Gede Rista Gayatri, Anak Agung Sagung Intan Gea, Octo Berkat Gracemon Loe Mau, Gracemon Handayani, Ni Made Hapsari Mahatmi HESTI YOHANA GINTING HESTIN YUSWANTI I Dewa Made Nurja Sadhi I Gede Indrawan I Gede Made Sunu Satwika Nur Agung I GEDE PUTU WIRAWAN I Gusti Ketut Suarjana I Made Damriyasa I Made Merdana I Made Robi I Made Sukada I Nyoman Arsana, I Nyoman I NYOMAN MANTIK ASTAWA I Nyoman Polos I NYOMAN RAI I NYOMAN WIJAYA I Putu Agus Hendra Wibawa I Putu Sampurna I Putu Sampurna I Wayan Batan I Wayan Masa Tenaya, I Wayan Masa I Wayan Suardana I Wayan Wahyudi, I Wayan I WAYAN WIRAATMAJA Ida Ayu Pasti Apsari Ida Ayu Putri Darmawati Ida Ayu Putu Aselya Mardyawati Ida Ayu Resmihariningsih IDA BAGUS GDE PRANATAYANA Ida Bagus Komang Ardana Ida Bagus Ngurah Swacita Joanita Auxilia de Sa Pereira Julita Dewitri Merthayasa Kadek Karang Agustina Kalimutu, Putu Krisnawan KARTINI HUTASOIT KETUT AYU YULIADHI Ketut Budiasa Ketut Tono Pasek Gelgel KHAMDAN KHALIMI Krisis Mariani Buulolo Lely Anggriani Nababan Luh Putu Pradnya Swari LULUK WULANDARI Mamboran, Nikko Marthen Manullang, Prila Kartika Maria Yasinta Manuama Martha Putri Manullang Mas Djoko Djoko MAS DJOKO RUDYANTO Matilde Fatima Correia Maulina Nababan, Maulina Mintari, Gusti Ayu Willem Munthe, Lambok Jenny Sagita Narty, Yasinta Ni Ketut Ayu Juliasih Ni Komang Alit Astiari Ni Luh Gede Sudaryati Ningsih, Gusti Ketut Asti Dea NOER SYAIFUL HAKIM Nurul Masyita, Nurul Pande Made Satya Sumitra Paramita, Ni Made Diana Pradnya Parwata, I Made Oka Prabarini Hanum Sari Pranata, Kadek Dwipayana Purnama Layli, Purnama Putra, I Made Arya Udyana Reny Navtalia Sinlae REZKI ABADI BONTONG Richard Stenly Tindjabate Rindang Dwiyani Rizki, Muhamad Farrel Rusmita, Gst Ayu Putu Ika Yulia Sabaaturohma, Clara Luceatriani Sanjaya, I Putu Wahyu Saputra, I Dewa Ketut Ari Sasadara, Maria Malida Vernandes Satria Yanuwardani Setiawan Shchegolkova, Nataliya Sidabutar, Febyana Sihombing, Vivi Ekatry SISKA MAHARGIAN FIBRIANTI Sonia Dewi Citra Sembiring Sosiawan, I Gede Arya Mas Sousa, Rojelio Dias Trindade Sudiartawan, I Putu Sukardika, Ketut Elok Sumarya, I Made SURAINIWATI SURAINIWATI Tampubolon, Immanuel Anthonio Caesebo Tjokorda Sari Nindhia TRI KOMALA SARI TRISNA AGUNG PHABIOLA Uli Rehlitna Sembiring, Uli Rehlitna UTAMA, I WAYAN EKA KARYA Wahyu Maha Putra, Anak Agung Gede Agung Widayantari, Anak Agung Ayu Sauca Sunia Wilson Lois, Wilson Wisnu Pradana YUDHA, I KADEK WISMA Yunita Sri Hastuti, Yunita Sri Yuyun Fitriani Zhao, Tiejun