Claim Missing Document
Check
Articles

Pengaruh Beberapa Jenis Lampu terhadap Hasil Tanaman Selada Keriting Hijau (Lactuca sativa L.) pada Plant Factory Rusmita, Gst Ayu Putu Ika Yulia; Suada, I Ketut; Wirawan, I Gede Putu
Agrotrop : Journal on Agriculture Science Vol 13 No 3 (2023)
Publisher : Fakultas Pertanian Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/AJoAS.2023.v13.i03.p05

Abstract

Effect of Several Types of Lights on Yield of Green Curly Lettuce (Lactuca sativa L.) at Plant Factory. Light intensity is the most important factor in the process of plant growth, light sources that can be received by plants other than the sun can also use light. This research took place from March to April 2022. This research was conducted at the Plant Factory, Faculty of Agriculture, Jl. Moyo Island No. 16X, Pedungan, South Denpasar. In this research experiment the design used was a completely randomized design (CRD) with 6 types of lamp treatments, namely: LED grow light, white LED lamp, yellow fluorescent lamp, white fluorescent lamp, combination of white, yellow, far-red HPL lamps and white fluorescent lamps. HPL combination of red, blue, yellow and repeated 4 times. The results of statistical analysis showed that the type of lamp treatment had a very significant effect on all observed variables, except number of leaves, and root dry weight. The type of lamp treatment had an effect on the yield of lettuce grown at the plant factory and was indicated by the total fresh weight with a value of (171.50 g) and total dry weight with value of (5.09 g) which were significantly different from other treatments. The best type of lamp for lettuce growth and yield is the best yellow fluorescent lamp.
Intraspecific SSR Marker Screening for Detection of Dendrobium crumenatum Mutants Generated from In Vitro Gamma Irradiation Dwiyani, Rindang; Sanjaya, I Putu Wahyu; Yuswanti, Hestin; Darmawati, Ida Ayu Putri; Suada, I Ketut; Manullang, Prila Kartika; Fitriani, Yuyun
Journal of Tropical Biodiversity and Biotechnology Vol 9, No 2 (2024): June
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jtbb.89896

Abstract

Determination of D. crumenatum mutant obtained from in vitro mutation breeding needs a long time due to its long-life cycle. SSR molecular markers can be used for early mutant detection. Specific SSR markers developed in D. crumenatum are not yet available. Alternative published SSR markers were developed from D. catenatum. The aims of this study are to screen the most informative SSR markers generated from D. catenatum tested in irradiated D. crumenatum population and to determine the gamma irradiation dose resulting the most mutants. Ten SSR markers were randomly selected and tested in 25 individuals of D. crumenatum plantlets irradiated with several doses (0, 5, 10, 15, and 20 Gy; 5 plantlets each dose). The result showed 7 of 10 primers were polymorphic and the other three were  monomorphic. All seven polymorphic primers can be used to identified intraspecific variation in the D. crumenatum mutant population. Markers dnsr28 and dnsr98 were the most informative, with the highest polymorphic information content (PIC) value of 0.5. Irradiation D. crumenatum protocorms using 10-15 gray doses were detected as the highest mutant percentage obtained up to 100% in the sample tested. This resulting marker information can be used to screen wider mutant population to decrease the non-mutant individuals in the population for maintenance and cost efficiency. The 10-15 Gy can be used as reference doses for gamma irradiation in 3 months old D. crumenatum protocorm materials. 
Phytochemical Content Identification And Antioxidant Activity Test Of Ethanol Extract Parijata Fruit (Medinilla Speciosa Blume) In Bedugul Area, Bali, Indonesia Mintari, Gusti Ayu Willem; Suada, I Ketut; Phabiola, Trisna Agung; Wijaya, Nyoman; Yuliadhi, Ketut Ayu; Bakar, Nor Kartini Abu
International Journal of Biosciences and Biotechnology Vol 11 No 1 (2023): International Journal of Biosciences and Biotechnology
Publisher : Central Laboratory for Genetic Resource and Molecular Biology, Faculty of Agriculture, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/IJBB.2023.v11.i01.p04

Abstract

Antioxidants are compound that function as free radical scavengers, these compounds can be found in parijata fruit (Medinilla speciosa Blume). The secondary metabolites of this fruit can be identified by the GC-MS method. The aims of study to identification of phytochemical content and test the antioxidant activity of the ethanol extract of Parijata fruit (Medinilla speciosa Blume) in the Bedugul area of Bali. This method is used because it is more sensitive and the results of the analysis are easy to understand. Using the GC-MS method, 11 organic compounds with a quality value of ? 90 were found in the ethanol extract of parijata fruit which have antioxidant, antimicrobial, and others. The 3 compounds with the highest Area Under Curve were 5-Hydroxymethylfurfural (AUC 31.05%); 1,2,3- Benzenetriol (AUC 22.32%); and 4H-pyran-4-one-2,3-dihydro-3,5-dihydroxy-6-methyl- (AUC 6.97%). These three compounds have antioxidant properties. Parijata fruit antioxidant activity test was tested with the DPPH method. The antioxidant activity of the ethanol extract of parijata fruit has an IC50 value of 11.26 mg/L, in the category of very strong antioxidant activity. So that with this category, parijata fruit can be given further treatment as food for health. In addition, compounds with antimicrobial properties can be used as biopesticide products in agriculture.
Characteristics of Indigenous Bacterial Isolates from Cocoa Plantations in Meko Village, Central Sulawesi, with Ability to Degrade Cellulose Shchegolkova, Nataliya; Kalimutu, Putu Krisnawan; Wirawan, I Gede Putu; Suada, I Ketut
International Journal of Biosciences and Biotechnology Vol 11 No 2 (2024): International Journal of Biosciences and Biotechnology
Publisher : Central Laboratory for Genetic Resource and Molecular Biology, Faculty of Agriculture, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/IJBB.2024.v11.i02.p06

Abstract

Indonesia is the third largest cocoa bean producing country in the world after Ghana and the Ivory Coast with current Indonesian cocoa bean production of 600,000 tons per year. The quality of cocoa is still relatively low compared to the harvest potential that should be obtained, such as the Sulawesi III clone which provides a yield potential of 2-2.4 tons per hectare. This study aims to obtain indigenous bacteria from Meko village so that the role of each bacteria can be further investigated. The results of growing bacteria on LB media found 25 isolates which were observed to have quite varied morphology including round and irregular shapes, having rough and dull and slimy surfaces, opaque, cloudy and colored (yellow and reddish) and having flat and rising elevations. Most of the bacteria obtained were rod-shaped, namely 23 were gram negative and three were gram positive. The shape of the cell is dominated by the shape of the rod, about 19 are rod-shaped and the remaining six are round.
Identification and Antioxidant Activity Test of Strawberry Ethanol Extract Rosalinda and Osogrande (Fragaria x Ananassa) Varieties Enjelina, Ni Desak Gede Rista; Wijaya, I Nyoman; Suada, I Ketut; Phabiola, Trisna Agung; Utama, I Wayan Eka Karya; Yudha, I Kadek Wisma; Zhao, Tiejun
International Journal of Biosciences and Biotechnology Vol 11 No 1 (2023): International Journal of Biosciences and Biotechnology
Publisher : Central Laboratory for Genetic Resource and Molecular Biology, Faculty of Agriculture, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/IJBB.2023.v11.i01.p06

Abstract

Strawberries (Fragaria sp.) have many varieties spread across Indonesia. Pancasari Village, Buleleng is a strawberry producing area in Bali, there are several strawberry varieties cultivated by strawberry farmers in Bali, including the Rosalinda variety which has a sweet taste and the Osogrande has a sour taste. The purpose of this research is to determine the compounds contained in the fruits of these two varieties and to determine their antioxidant activity. Phytochemical analysis was performed using the GC-MS (Gas Chromatography-Mass Spectrometry) method, antioxidant activity was performed using the DPPH method. The results showed that the thick ethanol extract of (Fragaria x Ananassa) the Rosalinda and Osogrande varieties contained the same compound, namely 5-Hydroxymethylfurfural which has a function as an antioxidant, with 7 compounds that have known uses in the Rosalinda variety and 11 compounds in the Osogrande variety. The antioxidant activity of thick ethanol extract of the Rosalinda strawberry fruit is higher with IC50 of 10,05 ppm than the thick ethanol extract of Osogrande variety strawberry fruit with IC50 of 10,47 ppm, which means both are classified as having very strong antioxidant activity.
ANTIFUNGAL ACTIVITY OF SIAM CITRUS (Citrus nobilis L.) ESSENTIAL OIL AGAINTS Lasiodiplodia theobromae THE PATHOGEN OF BLENDOK DISEASE Pranata, Kadek Dwipayana; Wirawan, I Gede Putu; Wibawa, I Putu Agus Hendra; Suada, I Ketut; Wijaya, I Nyoman; Phabiola, Trisna Agung
International Journal of Biosciences and Biotechnology Vol 11 No 2 (2024): International Journal of Biosciences and Biotechnology
Publisher : Central Laboratory for Genetic Resource and Molecular Biology, Faculty of Agriculture, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/IJBB.2024.v11.i02.p08

Abstract

Siam citrus (C. nobilis L.) is a type of citrus that is most widely cultivated in Bangli Regency. The main disease that attacks citrus plants is blendok caused by the fungus L. theobromae. Based on research siam citrus peel contains essensial oils that have potential as antifungals. The aim of this study was to study chemical content of essensial oil from siam citrus peel and its activity as an antifungal againts L. theobromae. Essensial oil was destilled using hydro steam distillition and analyzed by means of GC-MS. Antifungal activiy testing was carried out using the agar-well diffusion method with concentrations of 1%, 10%, 25%, 50%, 75%, and 100%. Dithane M45 6 g/l and DMSO 10% were used as positive and negative controls. The results showed that the siam citrus peel from Kintamani, Bangli contains essensial oils with the main chemical components, i.e. D-limonene (57.26%), Beta-pinene (9.09%), and Beta-myrcene (4.03%) which has uses as an antifungal, additive, antitumor, asthma and allergy reliever, repellent, anti-inflammatory, antioxidant, anticancer, and antibacterial. Essential oil concentration of 25 to 100% can inhibit the growth of L. theobromae. The largest diameter of inhibition is shown at a concentration of 100% and the smallest diameter of inhibition is shown at a concentration of 25%. Keywords: C. nobilis L., essensial oils, GC-MS, L. Theobromae, antifungal
Adaptation of African Swine Virus in Non-Swine Cell Lines: A Preliminary Study for Vaccine Candidate Tenaya, I Wayan Masa; Agustina, Kadek Karang; Suada, I Ketut; Apsari, Ida Ayu Pasti; Sari, Tri Komala; Handayani, Ni Made; Widayantari, Anak Agung Ayu Sauca Sunia; Suardana, Anak Agung Komang; Sumarya, I Made; Arsana, I Nyoman; Sudiartawan, I Putu; Wahyudi, I Wayan; Juliasih, Ni Ketut Ayu; Sudaryati, Ni Luh Gede; Damriyasa, I Made
Jurnal Medik Veteriner Vol. 8 No. 1 (2025): April
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol8.iss1.2025.114-123

Abstract

African Swine Fever (ASF) is among the most detrimental infectious viral diseases in pigs causing approximately 100% mortality. The disease was first reported about 83 years ago in Africa before spreading to Europe in 1957 and Asia in 2010. An adequate vaccine generally containing live attenuated virus isolates prepared in swine macrophages to control the disease is currently unavailable. Therefore, this study aimed to use murine neuroblastoma (N2a) cells, non-swine cell lines, to adapt African swine fever virus (ASFV) isolates for vaccine preparation. ASFV isolate called BL21 obtained from Bali and East Nusa Tenggara was previously propagated in swine macrophages. However, virus was currently adapted in the N2a cells to avoid unwanted issues associated with using swine macrophages, including microbial contamination, as well as technically laborious and ethical issues. The adapted BL21 was re-confirmed with quantitative polymerase chain reaction (q-PCR) and tested in vivo to examine the pathogenicity properties. The results showed that BL21 produced consistently and specifically positive q-PCR, killing experimental pigs with typical gross pathological changes of ASF. BL21 at a 10-3/mL dilution adapted in N2a cells showed similar antigenic properties causing the death of nearly 50% N2a cells in vitro and terminating all in vivo experimental pigs. In conclusion, the BL21 isolate reported in this study could be used as a vaccine candidate after more attenuation and particularly to determine a lethal dose of 50% (LD50) for future investigations.
Morphological Identification of Balinese Caulerpa Seaweeds Dewi, Ni Kadek Emi Sintha; Wirawan, I Gede Putu; Suada, I Ketut
Jurnal Biologi Tropis Vol. 24 No. 2b (2024): Special Issue
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v24i2b.7858

Abstract

Bulung boni and bulung anggur are species of Caulerpa seaweeds commonly found along the coastline of Serangan Island, Bali. Although these species have been widely utilized in various ways, formal species identification of these two macroalgae in Bali had not been conducted prior to this study. This research aims to characterize the morphological structures of bulung boni and bulung anggur through detailed morphological examination. Identification keys and previous studies were used to assist in the morphological identification process. The findings revealed that bulung boni shares morphological characteristics with Caulerpa cylindracea, while bulung anggur is morphologically comparable to Caulerpa macrophysa. These results provide valuable insights into the species composition of seaweeds in the region and contribute to a better understanding of local biodiversity.
DNA Barcoding Based on matK Gene and Phytochemistry Analyses of Local Balinese Kayu Tangi ( Lagerstroemia sp.) Suada, I Ketut; Wirawan, I Gede Putu; Ningsih, Gusti Ketut Asti Dea; Wijaya, I Nyoman; Munthe, Lambok Jenny Sagita; Gayatri, Anak Agung Sagung Intan; Sasadara, Maria Malida Vernandes; Parwata, I Made Oka
Biosaintifika: Journal of Biology & Biology Education Vol. 16 No. 3 (2024): December 2024
Publisher : Universitas Negeri Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v16i3.9265

Abstract

Kayu tangi (Lagerstroemia sp.) is one of the medicinal plants that is often used traditionally by Balinese people for treating insomnia, diabetes, and dysentery. This study aims to determine the phylogenetic trees and the active compounds in Lagerstroemia tomentosa extract and its antioxidant activities. The methods used in molecular identification are DNA isolation, PCR, and DNA sequencing. The DNA sample was BLASTed to see its homology with the sequences in GenBank. These sequences were then used for phylogenetic tree analysis. The phytochemical analysis was done using the GC-MS method and the antioxidant activity test used the DPPH (1,1-diphenyl-2-picrylhydrazyl) after being reacted with the tested extract.  The results of molecular identification showed that the samples used were closely related to Lagerstroemia tomentosa species (MW044208.1). The antioxidant activity test results showed an IC50 of 2.1 ugml-1 which is included in the very strong category. The phytochemical test results showed that the most dominant compounds contained in the plant stem bark were furfural (AUC 8.54%), beta-sitosterol (AUC 6.00%), and gamma-sitosterol (AUC 3.81%). Based on PubChem data, these compounds have antioxidant and antimicrobial properties as well as insecticide and herbicide. In conclusion, kayu tangi is very close to  Lagerstroemia tomentosa and it’s potentially used as a medicine with strong antioxidant activities.  
Co-Authors Agnes - Tasia Agung Denlira Sitepu AGUSTINA BORU MANIK Ahmat Fansidar Aletha Yuliana Mandala, Aletha Yuliana Alhamdani, Yusuf Riska Alvionita Alexther Lingga Anak Agung Komang Suardana Anastasia Cornelia Anastasya, Sindika Anggara Fajri Prasafitra ANITA KAROLINA SIMAMORA Apriyani, Ester Muki Arhiono, Haru Nira Putra Bakar, Nor Kartini Abu Cesarina Pascalia dos Santos Alegria Chandra Immanuel Saragih Christine Regina Fenita Wenno, Christine Regina Fenita Cita, I Putu Giri Wahyu Eka Dayana, Anak Agung Putri Isadela Dewi, Ni Kadek Emi Sintha Dimas Indra Dwi Purnama Dwi Yoga Sutrisna Dyah Ayu Widiasih Eggy Hidta Lusandika ELIYA KUSUMAWATI Elly Hariati Br Purba, Elly Hariati Br Emerensia Patryconsitha Aman Enjelina, Ni Desak Gede Rista Gayatri, Anak Agung Sagung Intan Gea, Octo Berkat Gracemon Loe Mau, Gracemon Handayani, Ni Made Hapsari Mahatmi HESTI YOHANA GINTING HESTIN YUSWANTI I Dewa Made Nurja Sadhi I Gede Indrawan I Gede Made Sunu Satwika Nur Agung I GEDE PUTU WIRAWAN I Gusti Ketut Suarjana I Made Damriyasa I Made Merdana I Made Robi I Made Sukada I Nyoman Arsana, I Nyoman I NYOMAN MANTIK ASTAWA I Nyoman Polos I NYOMAN WIJAYA I Putu Agus Hendra Wibawa I Putu Sampurna I Wayan Batan I Wayan Masa Tenaya, I Wayan Masa I Wayan Suardana I Wayan Wahyudi, I Wayan Ida Ayu Pasti Apsari Ida Ayu Putri Darmawati Ida Ayu Putu Aselya Mardyawati Ida Ayu Resmihariningsih Ida Bagus Komang Ardana Ida Bagus Ngurah Swacita Julita Dewitri Merthayasa Kadek Karang Agustina Kalimutu, Putu Krisnawan KARTINI HUTASOIT KETUT AYU YULIADHI Ketut Budiasa Ketut Tono Pasek Gelgel Lely Anggriani Nababan Luh Putu Pradnya Swari LULUK WULANDARI Mamboran, Nikko Marthen Manullang, Prila Kartika Maria Yasinta Manuama Martha Putri Manullang Mas Djoko Djoko MAS DJOKO RUDYANTO Maulina Nababan, Maulina Mintari, Gusti Ayu Willem Munthe, Lambok Jenny Sagita Narty, Yasinta Ni Ketut Ayu Juliasih Ni Luh Gede Sudaryati Ningsih, Gusti Ketut Asti Dea NOER SYAIFUL HAKIM Nurul Masyita, Nurul Pande Made Satya Sumitra Paramita, Ni Made Diana Pradnya Parwata, I Made Oka Prabarini Hanum Sari Pranata, Kadek Dwipayana Purnama Layli, Purnama Putra, I Made Arya Udyana Reny Navtalia Sinlae REZKI ABADI BONTONG Richard Stenly Tindjabate Rindang Dwiyani Rizki, Muhamad Farrel Rusmita, Gst Ayu Putu Ika Yulia Sabaaturohma, Clara Luceatriani Sanjaya, I Putu Wahyu Saputra, I Dewa Ketut Ari Sasadara, Maria Malida Vernandes Satria Yanuwardani Setiawan Shchegolkova, Nataliya Sidabutar, Febyana Sihombing, Vivi Ekatry SISKA MAHARGIAN FIBRIANTI Sonia Dewi Citra Sembiring Sosiawan, I Gede Arya Mas Sousa, Rojelio Dias Trindade Sudiartawan, I Putu Sukardika, Ketut Elok Sumarya, I Made SURAINIWATI SURAINIWATI TRI KOMALA SARI TRISNA AGUNG PHABIOLA Uli Rehlitna Sembiring, Uli Rehlitna UTAMA, I WAYAN EKA KARYA Wahyu Maha Putra, Anak Agung Gede Agung Widayantari, Anak Agung Ayu Sauca Sunia Wilson Lois, Wilson Wisnu Pradana YUDHA, I KADEK WISMA Yunita Sri Hastuti, Yunita Sri Yuyun Fitriani Zhao, Tiejun