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All Journal Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences International Journal of Biosciences and Biotechnology Jurnal Bios Logos Jurnal Natur Indonesia Jurnal Keteknikan Pertanian AL KAUNIYAH Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam Jurnal Penelitian Kehutanan Sumatrana Jurnal Riau Biologia Jurnal Pengabdian UntukMu NegeRI BIOLINK (Jurnal Biologi Lingkungan, Industri, Kesehatan) Jurnal Biologi Tropis Biosaintifika: Journal of Biology & Biology Education Bio-Lectura Jurnal Agronomi Tanaman Tropika (JUATIKA) Dinamika Pertanian Science, Technology, and Communication Journal
Dewi Indriyani Roslim
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Religion Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Economics, Econometrics & Finance Education Electrical & Electronics Engineering Engineering Environmental Science Immunology & microbiology Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Social Sciences

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DNA Barcoding Analysis of Betok Fish (Anabas testudineus) from Kampar, Riau Based on Cytochrome Oxidase Subunit I (COI) Rahmah, Miftahul; Hasibuan, Aldy Riau Wansyah; Melia, Tisha; Al Khairi, Hapiz; Roslim, Dewi Indriyani
Jurnal Biologi Tropis Vol. 24 No. 2 (2024): April - Juni
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v24i2.7121

Abstract

Ikan betok (Anabas testudineus, Bloch 1792) is one of fishes which is a member of family Anabantidae. Betok is an endemic fish in Riau Province. The scientific study about DNA barcoding of betok fish is still rarely. This research aims to analyze the DNA barcode sequence of cytochrome c oxidase I (COI) on betok fish. Methods were used in this study such as sampling, total DNA extraction, PCR, electrophoresis, sequencing and data analysis. The COI sequence studied had size of 694 bp. The BLASTn analysis showed that the betok fish had the highest similarity of 99.7% with A. testudineus and the lowest 93.00% with A. cobojinus. There was one nucleotide that characterize the betok fish based on the COI sequence namely nucleotide number 309. This study may enrich the DNA barcode database of betok fish in GeneBank.
Carbon Sequestration Potential of Traditional Agroforestry System in Rimbo Panjang Village, Kampar Based on Tree Biomass Isda, Mayta Novaliza; Fatonah, Siti; Yulminarti, Yulminarti; Roslim, Dewi Indriyani
Jurnal Biologi Tropis Vol. 24 No. 3 (2024): July - September
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v24i3.7344

Abstract

The traditional agroforestry industry is part of the Forestry and Other Land Use Net Sink 2030 (FOLU Net Sink 2030) strategy for low carbon development and climate resilience in Indonesia. This study aims to estimate the value of biomass, carbon stock, and carbon sequestration in traditional agroforestry systems in Rimbo Panjang Village, Kampar. Six plots (each measuring 20 x 20 meters) are used; three of the plots are 15 years old and three are 9 years old, respectively, for the conventional agroforestry system.  Biomass, carbon stock and carbon sequestration were estimated at the tree, pole and sapling levels.  Biomass calculations used allometric equations based on diameter at breast height (DBH) data. The results showed that carbon sequestration in Dusun 2 (618.2 tons/ha) was greater than in Dusun 1 (399 tons/ha), with an average carbon sequestration in both dusun of 508.6 tons/ha.  Trees contribute the highest value of biomass, carbon stock, and carbon sequestration, which is 74%, The difference in the value of biomass, carbon stock, and carbon sequestration in traditional agroforestry systems in Rimbo Panjang Village is significantly influenced by the average stem diameter. The various trees that make up traditional agroforestry in Rimbo Panjang Kampar, which is generally in the yard, should ideally be maintained and developed because of their high carbon sequestration potential.
DNA Barcoding Analysis Kitolod (Hippobroma longiflora) from Riau Based on matk Gene Herman, Herman; Sari, Mayang; Multivasari, Nella; Roslim, Dewi Indriyani
Jurnal Biologi Tropis Vol. 25 No. 1 (2025): Januari - Maret
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v25i1.8479

Abstract

The kitolod plant (Hippobroma longiflora) is a traditional medicinal plant originating from the Campanulaceae family. DNA barcoding is a technique for identifying an organism using short nucleotide sequences known as DNA barcoding. One of the DNA barcodes in plants is matK. This research aims to analyze DNA barcode sequence in the matK region of the kitolod plant using the DNA barcode. Samples were taken from the area of Tarai Bangun Village, Kampar Regency, Riau Province, Indonesia as many as two different individuals. The research stages carried out are sampling, followed by DNA isolation using the Geneaid Mini Plant kit, PCR follow thermo scientific instructions, gel agarose electrophoresis, sequencing, and data analysis using the bioinformatics program, namely BioEdit, BLASTn, and MEGA 6.0. The matK sequence of the kitolod plant obtained was 841 bp. The result showed that no identity value was found to reached 100%. The highest identity value (99.88%) was found in H. longiflora NC_035361.1. 34 nucleotide variations were found with one critical nucleotide for H. longiflora from Riau and six critical nucleotides for the H. longiflora. The matK DNA sequence from Kitolod Riau in this study is the first sequence reported in database GenBank.
Co-Authors ', Herman ', Tasiah , Deviona, , , HERMAN Abd. Rasyid Syamsuri Al Khairi, Hapiz Aldri Frinaldi ALEX HARTANA Altuhaish, Adeel Abdulkarim Fadhl Andariyusti, Felly Andriani, Lestari Anna Safarrida, Anna Arief Priyadi Ashfira Ashfira Ashfira, Ashfira Asih, Hastini Aslim Rasyad Aslim Rasyad At-Thahirah At-Thahirah Awitdrus Awitdrus, Awitdrus Azrial Azrial Azrial, Azrial Budiono, Deanne Yoshe Fidela Cahyati, Isa Endar Ciska Vivian Sianturi Defrianto Defrianto Desriani Ritawati Hutagalung Dilla Mutiarawati Dini Septya Nastiti Dita Deanesia Dodi Frianto, Dodi Endah Budi Lestari ENNIE CHAHYADI, ENNIE Fadel Nugraha Faizah, Niswah Fifi Puspita Fitriani, Ana Fitriyatun Nisa, Fitriyatun Frederika Sinuraya Furqoni, Aries Tri Hadiwiyono Hadiwiyono HAJRIAL ASWIDINNOOR Hasibuan, Aldy Riau Wansyah Herman Herman Herman Herman - Herman - Herman Herman Herman Herman Herman Herman Hestia Hairima Hutagalung, Desriani Ritawati Imra Atul Uswah Ingga Yurisna Fitriani Intan Sari Nuraini Lambok Nia Natalya Larissa Anggisti Lestari Andriani Linda Novita Liza Aulia Yusfi Martupa Nainggolan Mayang Sari Mayta Novaliza Isda Miftahudin . Miftahul Rahmah Muhamad Fauzan Multivasari, Nella Mutiarawati, Dilla Nery Sofiyanti Niswah Faizah Nur Aisyah Nur Aisyah Nurin Nuryani NURKHAIRANI, PUTRI Nuryani Nuryani PATMASARI, MUDRIKA Putri Agustina PUTRI PANJAITAN, KHAIRIZA UMAMI Putri, Fatma Jumaita Putri, Shalsadila Rahmadani Rahmi Anandia Rio Riduan Rosmeilinda, Tio Fanny Roza Elvyra Saktioto Saktioto SARAGIH, JESSICA RODEARNI Siti Fatonah Siti Khumairoh, Siti Siti Nurhayati Sri Wahyuningsih Sugeng, Santoso Suha Maudina Berampu Suhardi Suhardi Suhardi Suhardi Suharyanto Suharyanto Susilo Hambeg Poromarto Tio Fanny Rosmeilinda Tisha Melia Utut Suharsono Vera Magdaleni Manullang Wahyu Lestari Wahyu Lestari Yolla Putri Ardilla Yolla Putri Ardilla YULMINARTI, YULMINARTI Yundari, Yundari Yusfi, Liza Aulia