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Hamdani Budiman
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology Veterinary

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19. Histological of Thymus in Tegal Duck (Anas javanicus) at Different Ages Hamdani Budiman; Anugrah Septian; Hamny Hamny; Zainuddin Zainuddin; Cut Dahlia Iskandar; Mustafa Sabri; Muhammad Hambal; Abdul Harris
Jurnal Medika Veterinaria Vol 13, No 1 (2019): J. Med. Vet.
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.med.vet..v13i1.3523

Abstract

The aim of this research was to observe the histological of the thymus in tegal duck (Anas javanicus) according to the variation of ages. The animals used in this research were one month, two months and three months old of tegal duck. The thymus of each duck was analyzed by Hematoxylin-Eosin (HE) method and then observed under a light microscope. The result of the research performed differences in thymus structure of these three ages. The cortex had increased at the age of 2 months and decreased at the age of 3 months. The longest diameter of medulla at the age of 3 months was followed by ages 2 months and 1 month of tegal ducks.  The distribution of Hassall's body had increased at every age level. So, the cortex thickness decreased at the age of 2 months to 3 months, while the diameter of the medulla was getting longer.
PENGAMATAN LESI MAKROSKOPIS PADA HATI AYAM BROILEYANG DIJUAL DI PASAR LAMBARO ACEH BESAR DAN HUBUNGANNYA DENGAN KEBERADAAN MIKROBA Hamdani Budiman; T. Reza Ferasyi; Tapielaniari -; M. Nur Salim; Ummu Balqis; Muhammad Hambal
Jurnal Medika Veterinaria Vol 9, No 1 (2015): J. Med. Vet.
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.med.vet..v9i1.2997

Abstract

Penelitian ini bertujuan mengetahui hubungan antara lesi makroskopis dengan keberadaan mikroba pada hati ayam broiler. Sampel ayam broiler diperoleh di pasar Lambaro Aceh Besar dengan memilih lima hati ayam normal dan lima hati ayam yang mengalami lesi untuk dilakukan uji mikrobiologis. Hati diambil seberat 25 g, kemudian dilakukan stomaker untuk mendapatkan ekstrak pengencerannya. Selanjutnya dilakukan uji mikrobiologis, pengamatan dilakukan selama 24 jam dengan menghitung koloni per cawan petri. Hasil dari penelitian menunjukkan sampel hati ayam normal memiliki koloni masing-masing sebanyakn Sn21 (6,5x1022), x Sn2 (2,3x103), Sn3 (1,3x103), Sn4 (1,5x103), dan Sn5 (2,3x102) Cfu/ml, sedangkan sampel hati yang mengalami lesi memiliki koloni masing-masing sebanyak SL1  (3,1x104), SL2 (1,8x106), SL3 (2,7x105), SL4(4,5x106), dan SL5 (1,2x107 )Cfu/ml. Berdasarkan hasil pengamatan disimpulkan bahwa hati yang mengalami lesi cenderung memiliki jumlah mikroba yang lebih tinggi dibandingkan dengan hati yang normal.
PENGARUH PEMBERIAN KACANG PANJANG (Vigna unguiculatTERHADAP STRUKTUR MIKROSKOPIS GINJAL MENCIT (Mus musculus) YANG DIINDUKSI ALOKSAN Atika Resty Handani; M. Nur Salim; Abdul Harris; Hamdani Budiman; Zainuddin -; Sugito -
Jurnal Medika Veterinaria Vol 9, No 1 (2015): J. Med. Vet.
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.med.vet..v9i1.2987

Abstract

Penelitian ini bertujuan mengetahui pengaruh pemberian kacang panjang (Vigna unguiculata) dengan pengenceran bervariasi terhadap struktur mikroskopis ginjal mencit yang diinduksi aloksan. Sampel dalam penelitian ini berjumlah 12 ekor mencit (Mus musculus) jantan dengan rata-rata bobot badan 25-40 g yang dibagi menjadi empat kelompok perlakuan. Kelompok pertama (K0) sebagai kontrol negatif, hanya diberi akuades. Kelompok K1, K2, dan K3 diinduksi aloksan masing-masing 0,5 ml. Kelompok K1 sebagai kontrol positif. Kelompok K2 diberikan terapi 100 g kacang panjang yang dicampurkan dengan 50 ml akuades, sebanyak 0,5 ml. Perlakuan keempat (K3) diberikan terapi 100 g kacang panjang yang dicampurkan dengan 100 ml akuades, sebanyak 0,5 ml. Terapi diberikan pada pagi dan sore hari selama waktu 7 hari , secara oral dengan menggunakan sonde lambung. Pada hari ke-8 semua mencit dieutanasia dengan menggunakan kloroform dan diambil organ ginjal untuk pembuatan preparat histologis dan pemeriksaan struktur mikroskopis. Perubahan struktur mikroskopis diperiksa berdasarkan parameter sel glomerulus, sel tubulus hemoragi, peradangan dan nekrosis. Berdasarkan hasil pengamatan, pemberian kacang panjang pada kelompok K2 dan kelompok K3 dapat memperbaiki struktur glomerulus dan tubulus ginjal.
GAMBARAN DARAH TIKUS YANG DIINFEKSIKAN Trypanosoma evansi DAN DIBERI EKSTRAK DAUN SERNAI (Wedelia biflora) Dianatul Habibah Harahap; Yudha Fahrimal; Hamdani Budiman
Jurnal Medika Veterinaria Vol 7, No 2 (2013): J. Med. Vet.
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.med.vet..v7i2.2949

Abstract

Penelitian ini bertujuan mengetahui nilai packed cell volume (PCV), eritrosit,  dan leukosit darah tikus yang diinfeksikan Trypanosoma  evansi (T.evansi) dan diberi ekstrak daun sernai (Wedelia biflora). Hewan yang digunakan dalam penelitian adalah 25 ekor tikus jantan. Selama penelitian tikus diberi makan secara ad libitum. Rancangan yang digunakan adalah rancangan acak lengkap (RAL) pola searah dengan 5 kelompok perlakuan. Masing-masing kelompok perlakuan terdiri atas lima ekor tikus. Kelompok K0 tanpa infeksi T.evansi dan ekstrak sernai,kelompok KI diinfeksikan 103 T. evansi, kelompok KII diinfeksikan 103 T. evansi dan ekstrak daun sernai 30 mg/kg bobot badan, kelompok KIII diinfeksikan 103T. evansi dan ekstrak daun sernai 45 mg/kg BB, dan kelompok KIV diinfeksikan 103 T. evansi dan ekstrak daun sernai 60 mg/kg BB. Masing-masing kelompok diberi perlakuan selama 3 hari berturut-turut secara oral menggunakan sonde lambung. Hari berikutnya setelah selesai perlakuan, sampel darah diambil melalui ekornya. Nilai PCV dianalisis dengan metode mikrohematokrit dan untuk eritosit dan leukosit dianalisis dengan metode haemositometer. Rata-rata (±SD) nilai PCV darah tikus (%) pada K0, KI, KII, KIII, dan KIV berturut-turut adalah 40±0,7; 32±1,5; 35,2±6,9; 35±0,7; 34,8±3,5; eritrosit (106/ µl) berturut-turut adalah 7,62±2,6; 2,74±2,7; 4,98±1,9; 6,32±2,5; 7±1,9; dan leukosit (103 sel/µl) berturut-turut adalah 14,8±2,6; 42,2±2,7; 36,8±1,9; 34,2±2,5; 29,4±1,9. Dari hasil penelitian disimpulkan bahwa infeksi dari T. evansi berpengaruh terhadap gambaran darah tikus yang menyebabkan penurunan nilai PCV dan eritrosit sedangkan nilai leukosit mengalami peningkatan. Dosis 60 mg/kg BB ekstrak daun sernai mempunyai efek yang paling besar terhadap penurunan nilai PCV dan jumlah leukosit, akan tetapi meningkatkan jumlah eritrosit darah tikus yang diinfeksikan T. evansi dibandingkan dosis 45 dan 30 mg/kg.
10. Isolation and Identification of Lactic Acid Bacteria Genus Pediococcus from Sumatran Orangutan (Pongo abelii) faeces at Kandi Zoo and Kinantan Zoo West Sumatera Resti Reimena; Erina Erina; Darniati Darniati; Fakhrurrazi Fakhrurrazi; Darmawi Darmawi; Hamdani Budiman
Jurnal Medika Veterinaria Vol 11, No 1 (2017): J. Med. Vet.
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.med.vet..v11i1.3524

Abstract

This research has purposes to isolating and identifying the lactic acid bacteria genus Pediococcus in sumatran orangutan (Pongo abelii) feaces. Feaces are isolated from one orangutan at Kandi Zoo and two orangutans at Kinantan Zoo. The isolating of the lactic acid bacteria was done by using streak plate method with the MRSA (de Man Rogosa Sharpe Agar) specific media. Identification was done by the morphological observing, Gram stain, catalase test, oxidase test and biochemical test. The result showed that one isolate feaces from Kandi Zoo has cocci shaped with the isolate prediction approaches genus Pediococcus sp. The characteristic features of isolate are cocci shaped, Gram positive, tetrad cell structure, nonmotile, catalase negative, and oxidase negative. Based on these characteristics, it can be concluded that there is a lactic acid bacteria coloni which grouped into genus Pediococcus in male orangutan feaces from Kandi Zoo
PENGARUH KEPADATAN POPULASI TERHADAP GAMBARAN PATOLOGI ANATOMI DAN HISTOPATOLOGI INSANG IKAN NILA (Oreochromis niloticus) Eka Janni Haqqawiy; Winaruddin -; Dwinna Aliza; Hamdani Budiman
Jurnal Medika Veterinaria Vol 7, No 1 (2013): J. Med. Vet.
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.med.vet..v7i1.2914

Abstract

Penelitian ini bertujuan mengetahui gambaran patologi anatomi  ikan nila dan histopatologi insang ikan nila dengan kondisi kepadatan populasi pada wadah pemeliharaannya di atas normal. Lima puluh ekor ikan nila dibagi atas empat kelompok perlakuan yaitu kelompok kontrol terdiri atas 5 ekor ikan, kelompok I  10 ekor ikan, kelompok II 15 ekor ikan, dan kelompok III 20 ekor ikan. Insang difiksasi dalam larutan Davidson 10% kemudian dilakukan pembuatan sediaan histologi dengan menggunakan pewarnaan hematoksilin dan eosin (HE). Pengamatan histopatologi dilakukan dengan mikroskop cahaya biokuler, kemudian dilakukan pemotretan dengan fotomikrograf. Data yang diperoleh dianalisis secara deskriptif. Hasil pemeriksaan histopatologi ditemukan nekrosis, hiperplasia lamella primer, dan sekunder, serta fusi lamella pada insang ikan nilayang dipelihara dalam padat populasi di atas normal.
GAMBARAN HISTOPATOLOGIS LIMPA TIKUS PUTIH (Rattus novergicus) JANTAN DIINFEKSI Trypanosoma evansi DAN DIBERI EKSTRAK KULIT BATANG JALOH (Salix tetrasperma Robx) (Histopathological finding of Rat (Rattus novergicus) Spleen Infected with Trypanosoma evansi and Treated with Willow Bark (Salix tetrasperma Roxb) Extract) Elsa Mariane Ramadani; Hamdani Budiman; Sugito Sugito; Ummu Balqis; Muhammad Hambal; Razali Daud
Jurnal Medika Veterinaria Vol 9, No 2 (2015): J. Med. Vet.
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.med.vet..v9i2.3811

Abstract

The aim of this research was to determine the histopathological finding of rat (Rattus novergicus) spleen infected with Trypanosoma evansi and treated with willow bark (Salix tetrasperma Roxb) extract.This research used 25 male rats divided into four treatment groups and fed ad libitum. Group 0 (K0) was control group, group I (K1) was infected with 103/0.3 ml. T. evansi, group II (K2), III (K3), and IV (K4) were infected with103/0.3 ml T. evansi and treated with willow bark extract 30, 45, and 60 mg/kg bw, respectively. All groups were treated for 3 consecutive days orally using a stomach tube. On day four, rats were euthanized using chloroform and necropsied, then spleen were collected for histophatological examination. The results showed that all spleen on group K1, K2, K3, and K4 showed hyperplasia of white pulp, hemosiderin, and necrosis with different severity levels compared to normal spleen structure. In conclusion, the administration of willow bark extract at the dose of 30 mg/kg bw has a potency to prevent spleen from T. evansi infection. However, the higher the dose of willow bark extract the higher the level of spleen damage.Key words: spleen, T. evansi, willow bark extract
6. Identification of Parasites on Squid (Loligo spp.) in The Fish Auction Site (FAS) Lampulo Banda Aceh Resty Fauzana Rahman; Muttaqien Bakrie; Muhammad Hambal; Elia Wardani; Yudha Fahrimal; Dwina Aliza; Hamdani Budiman
Jurnal Medika Veterinaria Vol 11, No 1 (2017): J. Med. Vet.
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.med.vet..v11i1.4063

Abstract

A study has been conducted on squid (Loligo spp.) in the Fish Auction Site (FAS) Lampulo Banda Aceh, in March 2016 to study and identify parasites that infect the squid as well as determine the level of the prevalence of parasites. A total 20 squid were used as samples and examined in the Laboratory of Parasitology of Veterinary Faculty Syiah Kuala University. The ectoparasite examination on the surface of the body was conducted using body scraping on mucous and skin that were observed under microscope, and the endoparasite examination conducted by observation the abdomen of squid that was placed on the petridish and observed using microscope. The study revealed that one genus of endoparasite that infected squid was Anisakis sp. with prevalence of 5%.
GAMBARAN HISTOPATOLOGIS HATI TIKUS (Rattus novergicus) YANG DIINFEKSI Trypanosoma evansi SETELAH PEMBERIAN EKSTRAK KULIT BATANG JALOH (Salix tetrasperma Roxb) (Histopathological Changes of Rat (Rattus novergicus) Liver Infected with Trypanosoma evansi and Treated with Willow Tree Bark Extract (Salix tetrasperma Roxb)) Mulya Fahmi; Yudha Fahrimal; Dwinna Aliza; Siti Aisyah; Hamdani Budiman; Muhammad Hambal
Jurnal Medika Veterinaria Vol 9, No 2 (2015): J. Med. Vet.
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.med.vet..v9i2.3824

Abstract

This research aimed to determine the effect of willow (Salix tetrasperma Roxb) tree bark extract on histopathological changes of liver of rat (Rattus novergicus) infected with Trypanosoma evansi. Liver of 25 male rat, Wistar strain, were used in this study and all rat were fed with commercial feed ad libitum. This research used completely randomized design (CRD) with 5 treatments, each treatment consisted of 5 replicates. Treatment I was negative control group without any treatment, treatment II were only infected with 103 T. evansi, treatment III, IV, and V were infected with 103 T. evansi and treated with willow tree bark extract with the dose of 30, 45, and 60 mg/kg bw, respectively. The administration of willow tree bark extract was carried out for 3 consecutive days orally using a stomach tube. All rats were sacrificed and necropsied then liver were collected for histopathological examination. Percentage of histophatological changes found in hepatocytes of rat infected with T. evansi after the administration of willow tree bark extract in group P1, P2, P3, P4, and P5 were: normal hepatocytes 96.28%; 0.00%; 8.06%; 40.61%; and 0.00%; hydropic degeneration 0.00%, 44.98%, 58.00%, 38.29%, and 35.04%; fatty degeneration 0.00%, 0.00%, 0.00%, 0.00%, and 8.76%; necrosis 3.72%, 55.02%, 33.94%, 21.10%, and 56.20, respectively. In conclusion, the administration of willow tree bark extract is able to protect rat’s liver from T. evansi infection and the dose of 45 mg/kg bw is more effective than 30 mg/kg bw but the dose of 60 mg/kg bw of willow tree bark extract worsen the liver damage.Key words: histopathology, liver, T. evansi, willow
20. The Histological Structure of Tegal Duck (Anas javanicus) Spleen at Different Age Nabila Latifa Hafizsha; Hamdani Budiman; Hamny Hamny; Dian Masyitha; Cut Dahlia Iskandar; Ummu Balqis; Muhammad Jalaluddin
Jurnal Medika Veterinaria Vol 11, No 2 (2017): J. Med. Vet.
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.med.vet..v11i2.3522

Abstract

The aim of the  study was to determine the histological structure of tegal duck (Anas javanicus) spleen according to ages variation. One month, 2 month and 3 months of tegal duck were used as the sample of this study. The spleen of each duck was analyzed by Hematoxylin-Eosin (HE) method, then observed under light microscope. The result of this study performed there were the differences of histological structure of the ducks spleen. The capsule thickness had increased along with increasing age, which was 16.0 ± 1.0 µm; 25.3 ± 2.5 µm; and 29.0 ± 1.0 µm. The boundary between the two pulp in the tegal duck 3 months  old more obvious than 1 month or 2 months old. The diameter of white pulp had increased in the oldest duck. It can be concluded that the development of spleen histological structure was more perfect with the  increasing age of tegal duck.
Co-Authors . Darniati Abdul Harris Al Azhar Amiruddin . Amiruddin Amiruddin Anugrah Septian Atika Resty Handani Baehaqi Cut Dahlia Iskandar Darmawi Darmawi Dessy Florenstina BR. G Dian Masyitha Dianatul Habibah Harahap Dwina Aliza Dwinna Aliza Eka Janni Haqqawiy Elia Wardani Elsa Mariane Ramadani Erina Erina Fachrurrazi Fachrurrazi Fakhrurrazi Fakhrurrazi Fatin Syarifahnur Hamny Sofyan Has Nita Herrialfian - Hiqmah Laila Irwandi Yusuf Ismail Ismail M Nur Salim M. Hasan M. Nur Salim Mardhiah Abdian Mecky Desca Saad Muhammad Hambal Muhammad Hambal Muhammad Hanafiah Muhammad Hasan Muhammad Jalaluddin Mulya Fahmi Mustafa Sabri Muttaqien Bakrie Nabila Latifa Hafizsha Nurjannah Annah NURLIANA NURLIANA Razali Daud Razali Razali Resti Reimena Resty Fauzana Rahman Roslizawaty - Roslizawaty Roslizawaty Salim, Muhammad Nur Siti Aisyah Sugito - Sugito Sugito Sugito Sugito syafarina hanum T. Armansyah T. Fadrial Karmil T. Reza Ferasyi Tapielaniari - Teuku Reza Ferasyi Teuku Reza Ferasyi Teuku Shaddiq Rosa Ummu Balqis Widya Nanda Winaruddin Winaruddin Wulan Windasari Yudha Fahrimal Zainuddin - Zainuddin Zainuddin