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Optimasi Konsentrasi Ekstrak Alang-Alang (Imperata cylindrica L.) untuk Memacu Pertumbuhan dan Produksi Jagung Manis (Zea mays Saccharata Sturt) Mayta Novaliza Isda; Wahyu Lestari; Diana Agriani
Al-Kauniyah: Jurnal Biologi Vol 6, No 1 (2013): Al-Kauniyah Jurnal Biologi
Publisher : Department of Biology, Faculty of Science and Technology, Syarif Hidayatullah State Islami

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15408/al-kauniyah.v6i1.2829

Abstract

Sweet corn (Zea mays saccharata Struts) a type of corn of high economic value, not only used for consumption also used to make syrup. Imperata is one of the weeds that interfere with crop cultivation, but efforts continue to be made use of weeds as an addition to soil organic matter. The study was conducted to determine the concentration of the extract Imperata (Imperata cylindrica L.) appropriate in the sweet corn growth and determine the concentration of extract proper Imperata in the growth and production. The research method is a Completely Randomized Design (CRD) with 9 treatments and 3 replicates. The results showed that treatment of Imperata extracts with varying concentrations significantly influenced the growth of plant height and leaf number. But not significantly to the growth of the leaf length, leaf width, stem diameter, number of rows per cob, number of seeds per row and plant dry weight. At a concentration of 200 g / 250 ml was able to reduce the vegetative and generative growth of sweet corn.
PEMBENTUKAN TUNAS DARI BIJI MANGGIS (Garcinia mangostana L.) ASAL BENGKALIS DENGAN PENAMBAHAN BAP DAN MADU SECARA IN VITRO Mayta Novalida Isda; Siti Fatonah; Lia Novita Sari
Al-Kauniyah: Jurnal Biologi Vol 9, No 2 (2016): Al-Kauniyah Jurnal Biologi
Publisher : Department of Biology, Faculty of Science and Technology, Syarif Hidayatullah State Islami

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (399.09 KB) | DOI: 10.15408/kauniyah.v9i2.3376

Abstract

AbstrakGarcinia mangostana L. merupakan salah satu spesies dari famili Guttiferae kaya senyawa dari golongan xanthon. Manggis memiliki juvenil yang lama. Salah satu metode yang dapat memecahkan masalah dalam perbanyakan tanaman manggis dalam jumlah yang banyak, bisa sepanjang musim dan menghasilkan tanaman yang seragam yaitu kultur in vitro. Tujuan dari penelitian ini adalah untuk menentukan konsentrasi terbaik antara Benzil Amino Purin (BAP) dan madu terhadap induksi tunas dari eksplan biji manggis yang belah tiga melintang  secara in vitro. Penelitian ini menggunakan Rancangan Acak Lengkap (RAL) dengan perlakuan BAP (0;3 mg/L) dan madu (3;6 mg/L) pada media MS (Murashige-Skoog) dengan pengamatan 70 hari setelah tanam (hst). Hasil penelitian menunjukkan bahwa pemberian BAP maupun madu mampu meningkatkan pembentukan tunas. Pemberian BAP dan madu berpengaruh nyata terhadap persentase terbentuknya tunas, waktu muncul tunas, jumlah tunas dan panjang tunas.  Jumlah tunas terbanyak terdapat pada perlakuan 3 mg/L BAP sebanyak 20 tunas per biji. Kombinasi perlakuan dengan penambahan 3 mg/L BAP + 3 ml/L madu menghasilkan persentase terbentuk tunas tertinggi (100%); waktu muncul tunas tercepat 12,75 hst; dan panjang tunas tertinggi 1,86 cm. Pada penelitian ini telah berhasil menginduksi tunas dari biji manggis yang dibelah tiga melintang asal Bengkalis dengan penambahan BAP dan madu.AbstractGarcinia mangostana L. is one of the species within the family Guttiferae that rich in compounds from the class of xanthon. Mangosteen has time juvenile problems. One method that can solve the problem in the mangosteen plant propagation which can be produced in a large number in all season and uniform is in vitro culture. The purpose of this study was to determine the best concentration of BAP and honey to induce the formation of shoot from explants of mangosteen seeds split transversely into three pieces by in vitro method. This study used a Completely Randomized Design (CRD) with a treatment of BAP (0;3 mg/L) and honey (3;6 mg/L) on MS medium with 70 days of observation. The results showed that the BAP and honey can improve the formation of shoots. The addition of BAP and honey significantly affected the percentage of the formed shoots, the period of shoots appear, the number of shoots and shoot length. The treatment of 3 mg/L BAP resulted in the most shoots formed as many as 20 shoots per seed. The combined treatment 3 mg/L BAP + 3 ml/L of honey produced 100% of the formed shoots; fastest period of shoots appear (12.75 DAS); and the longest shoot (1.86 cm). Therefore, the addition of BAP and honey to the three transversely-split mangosteen seeds from Bengkalis origin successfully induced the formation of the shoot.
Induksi Akar pada Eksplan Tunas Anggrek Grammatophylum scriptum var. citrinum secara In Vitro pada Media MS dengan Penambahan NAA Dan BAP Mayta Novaliza Isda; Siti Fatonah
Al-Kauniyah: Jurnal Biologi Vol 7, No 2 (2014): Al-Kauniyah Jurnal Biologi
Publisher : Department of Biology, Faculty of Science and Technology, Syarif Hidayatullah State Islami

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (242.911 KB) | DOI: 10.15408/kauniyah.v7i2.2715

Abstract

Grammatophyllum scriptum var. citrinum is one variant of endangered from habitat destruction due to logging and forest fires and hunts by orchid collectors. The aim of this study was to determine the effect of BAP and NAA concentration in the root orchid propagation G. scriptum var. citrinum. The results showed that the application of BAP and NAA significant effect on the time of formation appeared roots (19 days), the best root number in the treatment of BAP 0.5 mg/l + 1.0 mg/l NAA for (5 pieces) and root length was obtained NAA 1 mg/l and a combination of 1.0 mg/l BAP + 0.5 mg/l NAA respectively 6.66 cm and 7.40 cm.
Potensi Ekstrak Daun Gulma Babadotan (Ageratum conyzoides L.) terhadap Perkecambahan dan Pertumbuhan Paspalum conjugatum Berg. Mayta Novaliza Isda; Siti Fatonah; Rahmi Fitri
Al-Kauniyah: Jurnal Biologi Vol 6, No 2 (2013): Al-Kauniyah Jurnal Biologi
Publisher : Department of Biology, Faculty of Science and Technology, Syarif Hidayatullah State Islami

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1097.606 KB) | DOI: 10.15408/kauniyah.v6i2.2752

Abstract

The allelopathic substances are an organic agents which produced and released by a plant that cause alteration on the neighboring plants. This research was aimed to determine the effect of Ageratum conyzoides L. leaf extract and also to determine optimum concentration on seed germination and growth of weed Paspalum conjugatum Berg.. This experiment used to extract concentration 0%, 5%, 10%, 15% and 20%. The experiment showed that germination and growth of Paspalum conjugatum Berg.were reduced and to happen damage by leaf extract of Ageratum conyzoides L. The highest germination, growth inhibition and demage at concentrations of the leaf extract 20% respectively are 80.5%, 63.15% dan 17,72 %.
Induksi Kalus dari Eksplan Daun In Vitro Keladi Tikus (Typhonium sp.) dengan Perlakuan 2,4-D dan Kinetin Marlina Agustina Sitinjak; Mayta Novaliza Isda; Siti Fatonah
Al-Kauniyah: Jurnal Biologi Vol 8, No 1 (2015): Al-Kauniyah Jurnal Biologi
Publisher : Department of Biology, Faculty of Science and Technology, Syarif Hidayatullah State Islami

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (291.973 KB) | DOI: 10.15408/kauniyah.v8i1.2703

Abstract

Rodent tuber (Typhonium sp.) belongs to Araceae that has a potential as medicinal plant. This procedure takes a long periode to produce a large quantity of plant. Therefore, an in vitro propagation using callus culture is necessary to solve this problem.  This research aimed to determine the best concentration of 2,4-D and kinetin to induce callus of in vitro leaf rodent tuber explants. This research used in vitro leaft explants of rodent tuber with MS medium.  The design used is Randomize Group Design with 10 treatment. The results showed that explants could not produce callus, the explants response just a swelling leaf explants. The treatment that could give up to 100% swelling response was 2.4-D 0.5 mg / L dan 1 mg / L and the combination treatment 0.5 mg / L 2.4-D + 0.3 mg /L kinetin, 0.5 mg / L 2.4-D + 0.5 mg / L kinetin and 1.5 mg / L 2.4-D + 0.5 mg / L kinetin.   The treatments that could maintain 66.67% explants growth was the combination 0.5 mg / L 2.4-D + 0.5 mg / L kinetin and 1 mg /L 2.4-D + 0.5 mg / L kinetin.
EMBRIOGENESIS SOMATIK DARI KALUS MANGGIS (Garcinia mangostana L.) ASAL BENGKALIS DENGAN PEMBERIAN BAP DAN MADU SECARA IN VITRO Tirtha Juliana; Mayta Novaliza Isda; Dyah Iriani
Al-Kauniyah: Jurnal Biologi Vol 12, No 1 (2019): Al-Kauniyah Jurnal Biologi
Publisher : Department of Biology, Faculty of Science and Technology, Syarif Hidayatullah State Islami

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15408/kauniyah.v12i1.5667

Abstract

AbstrakGarcinia mangostana L. dikenal dengan sebutan queen of the tropical fruits. Buah manggis terbentuk secara apomiksis yang bersifat rekalsitran. Salah satu cara perbanyakan tanaman manggis adalah dengan teknik kultur in vitro melalui embriogenesis somatik. Embriogenesis somatik manggis dilakukan dengan pembentukan kalus terlebih dahulu. Penelitian ini bertujuan untuk menentukan konsentrasi terbaik BAP dan madu secara tunggal serta kombinasinya dalam pembentukan embriogenesis somatik pada kalus biji manggis asal Bengkalis. Penelitian ini menggunakan rancangan acak kelompok (RAK) dengan pemberian konsentrasi BAP (3 dan 7 mg/L) dan madu (3, 6, dan 9 mL/L), secara baik tunggal maupun kombinasi, pada media Murashige-Skoog (MS) dengan 3 ulangan. Hasil penelitian menunjukkan bahwa pemberian BAP dan madu dalam seluruh perlakuan tersebut berpengaruh terhadap pembentukan fase-fase embriogenesis somatik kalus manggis. Konsentrasi terbaik dalam pembentukan fase embriogenesis somatik diperoleh dari perlakuan 3 mg/L BAP + 9 mL/L madu dengan presentase pembentukan kalus 100%, waktu muncul kalus 10,67 hst, volume kalus 1,33 dan adanya fase embriogenesis somatik berupa globular, hati, dan torpedo.Abstract Garcinia mangostana L. was known as the queen of the tropical fruits. Mangosteen was formed by apomixis which is recalcitrant. One of the methods of mangosteen propagation is by using a tissue culture technique through somatic embryogenesis. Mangosteen somatic embryogenesis occurs preceded by callus formation. This study aimed to determine the best concentration of BAP and honey in single as well as in combination for the formation phase of somatic embryogenesis in the callus of mangosteen from Bengkalis. The study used a randomized block design with the addition of BAP (3 and 7 mg/L) and honey (3; 6; and 9 mL/L) either single or combination in Murashige-Skoog (MS) medium with 3 replications. The results of this study indicated that the addition of BAP and honey in all treatments affected the phases of somatic embryogenesis of  mangosteen callus. The best concentration in the formation of somatic embryogenesis was obtained from, the treatment of 3 mg/L BAP + 9 mL/L which produced 100% of callus formation, with callus emergence time of 10.67 days after plantation, callus volume of 1.33 and the presence of somatic embryogenesis in the form of globular, heart, and torpedo.
Pemberian air rebusan kentang pada media Murashige-Skoog terhadap pertumbuhan protokorm anggrek sendu (Grammatophyllum stapeliiflorum) secara in vitro Fitria Elysye Ningrum; Mayta Novaliza Isda
Jurnal Ilmiah Pertanian Vol. 19 No. 1 (2022): Jurnal Ilmiah Pertanian
Publisher : Universitas Lancang Kuning

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31849/jip.v19i1.8514

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Grammatophyllum stapeliiflorum is one of the most popular orchid species because of its beauty and is characterized by the shape of the flower stalk that sticks down and the brown flowers with white and yellow spots that look like drops of water or sad. This type of orchid is almost extinct and is rarely found in its natural habitat, so it is necessary to perform in vitro propagation of orchids. The culture in vitro method is one of the most effective methods for propagating rare orchids. This study aimed to determine the effect of adding potato water on protocorm growth and determine the best concentration for the growth of G. stapeliiflorum protocorm in vitro. This study used a Completely Randomized Design (CRD) with the addition of potato water (concentrations 0, 50, 100, 150, and 200 ml/L) on Murashige-Skoog (MS) medium. The results showed that the percentage of live explants was 100% in all treatments. The addition of potato water to MS medium had no effect on the percentage of live explants but still affected the growth percentage of Protocorm-like bodies (PLBs) in vitro. The best concentration of potato water for the protocorm growth of G. stapeliiflorum in vitro to MS medium was at a concentration of 150 ml/L, which was indicated by the highest percentage of PLBs growth at 100% and the color of PLBs green (2.5GY 6/8) compared to other potato water treatments and controls.
KAJIAN MORFOLOGI DAN MIKROMORFOLOGI (SISIK SERTA TRIKOMA) 4 JENIS Pyrrosia Mirb. (Polypodiaceae) DI PROVINSI RIAU Nery Sofiyanti; Mayta Novaliza Isda
Jurnal Biologi Tropis Vol. 18 No. 2 (2018): Juli - Desember
Publisher : Universitas Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (507.07 KB) | DOI: 10.29303/jbt.v18i2.857

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Pyrrosia merupakan  golongan tumbuhan paku epifit dari family Polypodiaceae. Penelitian ini bertujuan untuk mengkarakterisasi morfologi (rhizoma, ptiolus dan lamina) dan mikromorfologi (sisik dan trikoma) dari 4 jenis Pyrrosia yang umum dijumpai di Provinsi Riau, yaitu Pyrrosia heterophyla, Pyrrosia lanceolate, Pyrrosia longifolia dan Pyrrosia pillosheloides.  Hasil karakterisasi morfologi menunjukan variasi pada organ yang diamati, terutama pada ukuran ptetiolus, bentuk dan ukuran lamina. Hasil pengamatan pada sisik menujukan varias pada warna sisik, jumlah rambut pada sisik dan posisinya. Sedangkan trikoma pada ptiolus dan lamina menunjukan persamaan bentuk yaitu stelata.
Phytochemical Contents of Underutilized Edible Plant from Riau Province, Ridan (Nephelium maingayi Hiern – Sapindaceae) Nery Sofiyanti; Mayta Novaliza Isda; Fitmawati Fitmawati; Asih Rahayu Ajeng Agesti; Ikhwan Taufik; Maya Sari; syafroni Pranata
Jurnal Biologi Tropis Vol. 21 No. 2 (2021): Mei - Agustus
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v21i2.2567

Abstract

Ridan (Nephelium maingayi) is one of Nephelium (Sapindaceae) member distributed in Riau Province. This underutilized edible plant is commonly found as wild species, and poses three different fruit morphologies (oval, asymmetric and rounded shaped fruit). The phytochemical contents of these fruit species had not been reported. This study aimed to identify the phytochemical contents of fruit parts (peel, aril and seed) of three different fruit shapes of N. maingayi (oval, asymmetric and rounded shaped fruit) from Riau Province. A total of six secondary metabolite groups were tested (alkaloid, terpenoid, steroid, flavonoid, saponin dan tannin), using qualitative analysis based on the presence of precipitation (alkaloid), the change of mixture color (steroid, terpenoid, flavonoid and tannin) and soapy foaming substance for saponin. The result showed that peels of Ridan (N. maingayi) gave the highest phytochemical contents (alkaloid, terpenoid, flavonoid, saponin and tannin.). Terpenoid and saponin were found in peel, aril and seed of three different fruit shapes. This study concluded that the phytochemical contents of three fruit shapes of Ridan (N. maingayi) varies among the different shapes of fruit. This result of this study provide the first information of bioactive profile of N. maingayi form Riau Province.
Karakteristik histokimia daun Ridan (Nephelium maingayi Hiern – Sapindaceae) dari Provinsi Riau Nery Sofiyanti; Fitmawati Fitmawati; Mayta Novaliza Isda; Asri Ria Lestari
Jurnal Biologi Udayana Vol 26 No 1 (2022): JURNAL BIOLOGI UDAYANA
Publisher : Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/JBIOUNUD.2022.v26.i01.p09

Abstract

Ridan (Nephelium maingayi) merupakan salah satu anggota famili Sapindaceae yang tersebar di Provinsi Riau, namun tidak banyak dibudi dayakan karena buah yang berukuran kecil dan agak masam. Pada penelitian ini bertujuan untuk mengetahui karakteristik histokimia daun ridan. Senyawa metabolit yang diuji adalah tannin, flavonoid, alkaloid serta lipid. Sampel yang digunakan adalah daun ridan yang sudah dewasa. Irisan sampel dilakukan menggunakan hand microtome, dan selanjutnya diuji dengan larutan FeCl3 (tannin), NaOH (flavonoid), Wagner (alkaloid) dan Sudan III (lipid). Sampel diamati dan didokumentasikan menggunakan mikroskop digital. Uji histokimia menunjukan bahwa bagian daun ridan (N. maingayi) pada umumnya mengandung tannin, alkaloid, lipid dan flavonoid. Uji lipid menunjukan hasil negatif pada jaringan kortek dan empulur tangkai anak daun, serta pada jaringan empulur ibu tangkai daun. Hasil kajian ini memberikan informasi tambahan pada kajian histokimia anggota dari marga Nephelium.
Co-Authors Adjie, Agung Purnomo Afragenia Zelfira Afrilliani, Aneka Agung Purnomo Adjie Agus Saputra Alvi, Rafi Shadiq Alzira Salsabillah Anwar, Nurhayati Appriliya Destiyana Asih Rahayu Ajeng Agesti Asri Ria Lestari Ayu Azhari Azzahwantara, Adil Damayanti, Frisca Desi Indriani, Desi Desita Salbiah Desna Tasya Rahmadhani DESTI ZARLI MANDARI, DESTI ZARLI Dewi Indriyani Roslim Dewi, Reza Puspita Dhavalyra, Salwa Dhini Yulianti Diana Agriani Diandra Prudentia Dwi Retno Sari, Dwi Retno Dyah Iriani EFENDI, RANNY ARIESTA Eki Susanto Elli Indriana Putri Elsya Desviyanti Elvianis Elvianis ENNIE CHAHYADI, ENNIE Erwina Juliantari ERWINA JULIANTARI, ERWINA Fahira, Nidia Fajar Nugraha Fariza, Isra Febby Ika Desyana Fibrianti, Chica Ollivia Fitmawati Fitmawati Fitmawati Fitmawati Fitmawati Fitmawati Fitria Elysye Ningrum Fransisca Elvarina Fransisco, Pangeran Saud Hanum, Meutia Hariono, Eko Haris Gunawan Ikhwan Taufik Ilahi, Rizky Nanda Kurnia Ismu Sodaqti Iwantono Iwan Barnawi KHAIRIJON, KHAIRIJON Lailani Sabrina Lia Novita Sari M. Athallah Dzikri Alhady M. Rizqy Pratama Syah Marlina Agustina Sitinjak Maulana Ishak Maya Sari Maya Sari Mimi Safitri Morsin, Marlia Muhammad Asyraf Muhammad Tamyis Lutfi Hakim Nadia Kristina Najmi Fadhila Nithami Nery Sofiyanti Nia Ivanka Noto Prasetio Nurhafidha Ramadhani Nurhayati Anwar Patar Sinaga Permata, Ariya Pranata, Syafroni Priyadika, Laura Aprilia Ayu Kusuma Wahyu Purwoko, Agus Putri Maisarah Qintara Sahira Rahmi Fitri Rana, Syafia Diang Ranny Wirmasari Rasyidah Ulfa Reza Puspita Dewi Riche Afrilla Riski Saputra Rizal Noor Rodesia Mustika Roza Rodesia Mustika Roza Ronald, Oriza Athalia Rosmaina Rosmaina Salsabilla, Melda Jannatul Saputra, Agus Sarah Novita Sari Umayah, Sari Sari, Fajrika Savanah Zahra Simbolon, Norsinta Ida Sinaga, Patar Sirait, Yudhistio Artia Perdamaian Siswiana Emila Siti Fatonah Siti Fatonah SRI CAHYATI, SRI Stefani, Dea Suhendra, Meyla Sujarwati Sujarwati Sujarwati Sujarwati Sujarwati Suratun Nafisah Surjawati, Surjawati Syafia Diang Rana syafroni Pranata Syahrul M. Syajali, Hidayati Tengku Emrinaldi Tirtha Juliana Titin Nilasari Titrawani Titrawani Titrawani, Titrawani Ulfatur Rohmah Vina Dwi Amanda Wahyu Lestari Wahyu Lestari Wahyu Ramadhan Wusqa, Wasila Yana Lisa Warni Yovita, Cici Yudani, Ananda Febri Yulia Rahmi YULMINARTI, YULMINARTI Yuniati Ziliwu, Friska Zulva, Naya Septia