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All Journal HAYATI Journal of Biosciences Jurnal MIPA Jurnal Akuatika Jurnal Natur Indonesia Majalah Kedokteran Bandung Cropsaver : Journal of Plant Protection Dharmakarya : Jurnal Aplikasi Ipteks Untuk Masyarakat AGRIVITA, Journal of Agricultural Science Jurnal Riset Kimia SCIENCE TECH: Jurnal Ilmiah Ilmu Pengetahuan dan Teknologi Al-Kimia Chimica et Natura Acta Indonesian Journal of Chemistry Jurnal Teknologi & Industri Hasil Pertanian Jurnal Pengolahan Pangan Molekul: Jurnal Ilmiah Kimia JURPIKAT (Jurnal Pengabdian Kepada Masyarakat) Jurnal Ilmiah Berkala: Sains dan Terapan Kimia Kartika : Jurnal Ilmiah Farmasi al Kimiya : Jurnal Ilmu Kimia dan Terapan Jurnal Sains dan Kesehatan Kimia Padjadjaran
Safri Ishmayana
Departemen Kimia, Fakultas Matematika Dan Ilmu Pengetahuan Alam, Universitas Padjadjaran
Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Dentistry Earth & Planetary Sciences Economics, Econometrics & Finance Education Engineering Environmental Science Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Physics Public Health Social Sciences

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Journal : Jurnal MIPA

 1 
Pengaruh Suplementasi Ion Logam Besi Terhadap Kinerja Fermentasi dan Toleransi Sel Ragi Saccharomyces cerevisae terhadap Cekaman Lingkungan Rachman, Saadah D.; Putri, Tysza Ainnunnisa Maulidya; Safari, Agus; Anggraeni, Nenden I.; Fadhlillah, Muhammad; Ishmayana, Safri
Jurnal MIPA Vol 9, No 2 (2020)
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35799/jmuo.9.2.2020.28565

Abstract

Selama proses fermentasi bioetanol, ragi Saccharomyces cerevisiae terpapar berbagai cekaman lingkungan. Ion logam yang berpotensi untuk meningkatkan kinerja fermentasi dan toleransi sel terhadap cekaman ialah ion logam besi II (Fe2+) yang berperan sebagai kofaktor dalam berbagai proses metabolisme. Tujuan dari penelitian ini adalah mempelajari pengaruh suplementasi ion Fe2+ dalam media fermentasi terhadap kinerja fermentasi, serta toleransi sel ragi terhadap cekaman etanol, oksidatif, asam lemah dan tekanan osmotik. Penelitian diawali dengan melakukan fermentasi glukosa menggunakan S. cerevisiae A12 selama 120 jam dengan pengambilan sampel dengan interval waktu 6 jam untuk 24 jam pertama serta 12 jam untuk sisanya. Masing-masing sampel ditentukan nilai OD600nm, persentase sel hidup, jumlah sel hidup, kadar glukosa, kadar etanol. Pada jam ke 24, sel diuji daya tahannya terhadap cekaman. Hasil penelitian menunjukkan bahwa suplementasi ion logam Fe2+ hanya mampu meningkatkan laju produksi etanol, tetapi tidak mempengaruhi parameter lain.During bioethanol fermentation process, Saccharomyces cerevisiae yeast cells are exposed to various environmental stress factors. One of metal ions that have potency for improving fermentation performance and yeast stress tolerance is ferrous ion (Fe2+) that acts as cofactors in various metabolism process. The present study was directed to investigate the effect of ferrous ion supplementation to the fermentation media on fermentation performance, improving yeast stress tolerance against ethanol, oxidative, weak acid and hyperosmotic stresses. The fermentation was conducted by fermenting glucose using S. cerevisiae strain A12 for 120 hours. The sampling was performed every 6 hours during the first 24 hours and 12 hours for the rest of fermentation. The sample was examined for their OD600nm, total cell number, viable cell number, glucose content and ethanol content. At 24 hours the cell was examined for their stress tolerance. The result of the present study indicates that supplementation using ferrous ion improve the rate of ethanol production, but not other parameters.
Co-Authors ', O. Suprijana Abubakar Sidik Abubakar Sidik Ace Tatang Hidayat Agus Safari Agus Safari Agus Safari Agus Safari Agus Safari Agus Safari Agus Safari Agus Safari Ahsanul Chaliqin Gayo Anastasya Firdausi Anggraeni, Nenden I. Anggraeni, Nenden Indrayati Anne Carolina Annisyaban Fatiha Azzahra Ari Hardianto Asep Juanda Budiman, Mulydayana Reanifah Carolina, Anne Ceppy Nasahi Danar Dono Dani Permana Desi Harneti Putri Huspa Devi, Puspitasari Rosiana Dian S Kamara Dian S. Kamara Dian S. Kamara Djajasoepena, Sadiah Djajasoepena, Sadiah DJOKO PRIJONO Ekawardani, Savira Endah Wulandari Fadhlillah, Muhammad Fadhlillah, Muhammad Fahruddin, Muhammad Fahmi Farhan Azhwin Maulana Fazl, Fazli Fazli Fazl Fitri Widiantini Frida F. Isnanisafitri Frida F. Isnanisafitri Frida F. Isnanisafitri Ghina, Sarah Fahma Ginting, Sani D. R. Br Gobel, Khaeriska Rahayu Hafiz, Ersanda Harahap, Winni Suryani Hari R. Haryadi Hasriani, Shela Amelia Herlian E. Putra Herlina Marta Hesti Lina Wiraswati Horasio, Debora Tamaris Husain Akbar Sumeru Ida Indrawati Idar Idar Idar Idar Idar Idar Idar Idar Idar, Idar Ika Wiani Iman Permana Maksum Isnanisafitri, Frida Febriani Juanda, Asep Kamara, Dian S Kamara, Dian S. Kamara, Dian S. Kindi Farabi Latifah, Rina G. Latifah, Rina Gaida Lia Faridah Linar Z Udin Linar Z Udin Linar Z. Udin M. Yusuf Awaluddin Mawalia, Khiftul Muhammad Fadhlillah Muhammad Fadhlillah Muhammad Fadhlillah Muhammad Fadhlillah Muhammad Fadhlillah Muhammad Yusuf Nabila, Najwa Nadia Maulida Nenden I. Anggraeni Nenden I. Anggraeni Neneng S Widayani Neneng Sri Widayani Nurisa Fadillah Isnaeni Nurlelasari Nurlelasari Nurrusyda, Fajriana S Nurrusyda, Fajriana Shafira Nurrusyda, Fajrina Shafira O. Suprijana O. Suprijana PUTRI, RAFIKA Putri, Rafika Nanda Putri, Tysza Ainnunnisa Maulidya R. Arif Malik Ramadhan R. Ukun M.S. Soedjanaatmadja Rachman, Saadah D Rachman, Saadah D. Rachman, Saadah D. Rachman, Saadah Diana Rani Maharani Regaputra Satria Janitra Ridya Widya Pasisca Roni Sutrisna Rudi Hartono Rusky I. Pratama Saadah D Rachman Saadah D. Rachman Saadah D. Rachman Saadah D. Rachman Saadah D. Rachman Saadah D. Rachman Saadah Diana Rachman Saadah Diana Rachman Saadah Diana Rachman Sadiah Djajasoepana Sadiah Djajasoepena Safari, Agus Sani Dwiningrum Rahayu Br Ginting Sani Dwiningrum Rahayu Br Ginting Savira Ekawardhani Septiana, Reysha A. Q. A. Shabarni Gaffar Shabrinna, Hanif Shahab, Ali Ridho Soetijoso Soemitro Soetijoso Soemitro Sofyan Multazam N Aji Sumeru, Husain A. Sumeru, Husain Akbar Suprijana, O. Supriyadi, Isma Yustifania Sutrisna, Roni Sylvi Qurrotul Aini Tambunan, Clara Junita Toto Subroto Tri Mayanti Trianing Tyas Kusuma Anggaeni Udin, Linar Z Unang Supratman Wahyu Widayat Westy Juniaty Widayani, Neneng Sri Wiliandini, Fadilla Yeni W Hartati Yeni Wahyuni Hartati Yogas Dwi Pratiwi Yusuf Hidayat Yusuf Hidayat Yusup Hidayat Yusup Hidayat Yusup Hidayat Zamzahra, Salma