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All Journal Pastura; Journal Of Tropical Forage Science Jurnal Ilmu Ternak Jurnal Ilmu-Ilmu Peternakan (Indonesian Journal of Animal Science) Kultivasi Jurnal Nutrisi Ternak Tropis dan Ilmu Pakan ZIRAA'AH MAJALAH ILMIAH PERTANIAN Agrinimal Jurnal Ilmu Ternak dan Tanaman Media Kontak Tani Ternak Agrivet: Jurnal Ilmu-ilmu Pertanian dan Peternakan JAS (Journal of Animal Science) Jurnal Peternakan Sabana Farmers: Journal of Community Services
Romi Zamhir Islami
Fakultas Peternakan Universitas Padjadjaran
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Energy Immunology & microbiology Industrial & Manufacturing Engineering Social Sciences Veterinary Other

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Eskalasi Pengetahuan Bahan Pakan dan Formulasi Ransum Ternak Ruminansia Bagi Peternak Milenial di Jawa Barat Christi, Raden Febrianto; Islami, Romi Zamhir; Febrianto, Firman; Sudrajat, Ajat
Farmers: Journal of Community Services Vol 6, No 1 (2025): Farmers: Journal of Community Services
Publisher : Unpad Press

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/fjcs.v6i1.60409

Abstract

Pemahaman bahan pakan dan formulasi ransum perlu ditingkatkan pada peternak ruminansia. Semakin mengetahui jenis bahan pakan maka akan tepat pula dalam proses penyusunan ransumnya. Tujuan dari penelitian ini adalah eskalasi pengetahuan tentang bahan pakan dan formulasi ransum menjadi solusi yang sangat diperlukan untuk meningkatkan produktivitas ternak ruminansia di Jawa Barat. Kegiatan telah dilaksanakan pada bulan November 2024 yang bertempat di Kota Bandung dan diikuti sebanyak 70 peternak milenial dari seluruh perwakilan Kabupaten di Jawa Barat. Metode yang digunakan dalam kegiatan ini diantaranya  tahapan persiapan, penyuluhan dan demonstasi, serta evaluasi pre-test dan post test. Langkah awal yang dilakukan meliputi identifikasi masalah, kajian literatur sebagai dasar pemecahan masalah, serta persiapan terkait perizinan, lokasi, dan jadwal pelaksanaan. Tahap selanjutnya mencakup penyuluhan dan demonstrasi. Hasil menunjukkan bahwa terjadi peningkatan kegiatan penyuluhan pada pengertian pakan  100%, bahan pakan ruminansia 100% yang semula 92,85%, penggolongan nutrien pakan 100% semula 85,71%,  kebutuhan nutrien pakan 93,33% semula 35% dan teknik formulasi ransum 93,33% semula 35,71%.  Terjadi peningkatan pengetahuan dan pemahaman terhadap bahan pakan dan formulasi ransum ternak ruminansai pada peternak milenial di Jawa Barat mulai pengertian pakan, bahan pakan, penggolongan nutrien pakan, penggunaan kebutuhan nutrien pokok dan teknik formulasi ransum.
KONSENTRASI DAN KEMURNIAN DNA HASIL EKSTRAKSI RUMPUT ODOT, JAGUNG HIBRIDA, PADI IR64 DAN PADI CIHERANG Rahmah, Vita Amalia; Tanuwiria, Ujang Hidayat; Islami, Romi Zamhir
Agrinimal Jurnal Ilmu Ternak dan Tanaman Vol 13 No 2 (2025): Agrinimal Jurnal Ilmu Ternak dan Tanaman
Publisher : Jurusan Peternakan Fakultas Pertanian Universitas Pattimura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/ajitt.2025.13.2.119-126

Abstract

Pemahaman terhadap karakteristik genetik tanaman sangat dibutuhkan dalam pengembangan varietas unggul guna mendukung ketersediaan pakan ternak berkualitas, yang salah satunya dapat dilakukan melalui analisis DNA. Penelitian ini bertujuan untuk menganalisis konsentrasi dan kemurnian DNA empat jenis tanaman pakan, yaitu rumput odot, jagung hibrida, padi IR64, dan padi Ciherang. Metode yang digunakan dalam penelitian ini meliputi ekstraksi DNA menggunakan Genomic DNA Mini Kit (Plant) dari Geneaid, diikuti dengan pengukuran konsentrasi dan kemurnian DNA menggunakan spektrofotometer NanoDrop. Hasil penelitian menunjukkan bahwa DNA dari seluruh sampel memiliki konsentrasi yang cukup untuk analisis molekuler, yaitu antara 12,5 hingga 75,9 ng/μL. Rasio A260/A280 menunjukkan bahwa DNA jagung hibrida, padi IR64, dan padi Ciherang memiliki tingkat kemurnian yang sesuai standar (1,8–2,0), sementara DNA rumput odot menunjukkan rasio kemurnian rata-rata 1,44 berada di bawah standar kemurnian dan mengindikasikan adanya kontaminasi protein. Dengan demikian, metode ekstraksi menggunakan kit dari Geneaid cukup efektif dalam menghasilkan DNA berkualitas dan siap untuk dilakukan analisis lebih lanjut meskipun untuk rumput odot perlu optimasi lebih lanjut guna memperoleh DNA dengan kemurnian optimal. ABSTRACT Understanding the genetic characteristics of these plants is essential for the development of superior varieties to ensure the availability of quality animal feed, which can be achieved through DNA analysis. This study aimed to analyze the concentration and purity of DNA from four types of forage crops: Pennisetum purpureum cv. Odot (odot grass), hybrid corn, IR64 rice, and Ciherang rice. The methods used included DNA extraction using the Geneaid Genomic DNA Mini Kit (Plant), followed by measurement of DNA concentration and purity using a NanoDrop spectrophotometer. The results showed that all samples yielded DNA with sufficient concentration for molecular analysis, ranging from 12.5 to 75.9 ng/μL. The A260/A280 ratio indicated that the DNA from hybrid corn, IR64 rice, and Ciherang rice met the standard purity range (1.8–2.0), while the DNA from odot grass showed an average purity ratio of 1.44, suggesting protein contamination. Therefore, the Geneaid extraction kit was effective in producing high-quality DNA suitable for further molecular analyses, although additional optimization is required for odot grass to obtain DNA with optimal purity.
Application of the DNA Molecular Markers RM20A and RM241 on King Grass (Pennisetum purpuphoides) and Dwarf Elephant Grass (Pennisetum pupureum cv. Mott) Islami, Romi Zamhir; Ari Zindhi, Muhammad Amrullah; Mansyur; Susilawati, Iin
Jurnal Ilmu-Ilmu Peternakan Vol. 34 No. 2 (2024): August 2024
Publisher : Faculty of Animal Science, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jiip.2024.034.02.15

Abstract

Feed, as the main element that provides livestock nutrition, is composed of two components, namely, concentrate and forage. Examples of commonly used forages are king grass and dwarf elephant grass. These grasses are very familiar among farmers because they have good crude protein (CP) content; specifically, the CP content of king grass is 13,50% (Siswanto et al., 2016), and the CP content of dwarf elephant grass is 14,35% (Erleli, 2020). Forage production can be disrupted by weather factors. In the dry season, the production of king grass and dwarf elephant grass decreases by 50%. As a result, there is a forage crisis in the dry season. To overcome this, genetic improvement of king grass and dwarf elephant grass is possible. Previous research has succeeded in finding primers RM20A and RM241, each of which can lead to new traits in rice plants, namely, leaf rolling and plant height. This primer can be applied to king grass and dwarf elephant grass to increase the production potential of these plants and increase plant resistance in the dry season. This research uses the PCR technique to amplify primers from the template DNA of king grass and dwarf elephant grass. Optimization was carried out via several procedures due to species differences among rice, king grass and dwarf elephant grass. The results revealed that the RM241 primer could not be amplified at all in king grass plants and was amplified only in dwarf elephant grass. Moreover, the RM20A primer produced better results, where amplification could occur in both plants. This may be due to several factors, such as the condition of the DNA template, the condition of the materials and the possibility of human error. Amplification of the two primers also revealed that they were polymorphic, indicating that these two primers could not accurately amplify SSRs in king grass and dwarf elephant grass. This also causes many smears in the visualization of the PCR results. Overall, this research was able to determine the plant height and leaf rolling characteristics of king grass and dwarf elephant grass.
Eksplorasi fitogenik daun kari Murraya koenigii L. Spreng sebagai pakan fungsional berkelanjutan: kajian literatur Nyimas Popi Indriani; Iin Susilawati; Lizah Khairani; Mansyur; Romi Zamhir Islami
Agrivet : Jurnal Ilmu-Ilmu Pertanian dan Peternakan (Journal of Agricultural Sciences and Veteriner) Vol. 14 No. 1 (2026): Juni
Publisher : Fakultas Pertanian Universitas Majalengka

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31949/agrivet.v14i1.18988

Abstract

Curry leaf plant (Murraya koenigii) is an aromatic plant from the Rutaceae family found widely in South and Southeast Asia. Used as a culinary spice, curry leaves have great potential as phytogenics (bioactive compounds), natural plant-based used in animal feed to improve health and productivity. The use of phytogenics is a safer alternative to antibiotic growth promoters (AGPs) because it can reduce the risk of microbial resistance and drug residues in livestock products. Bioactive compounds in curry leaves include carbazole alkaloids, flavonoids, saponins, tannins, phenolics, terpenoids, and essential oils, which act as antioxidants, antimicrobials, anti-inflammatories, immunomodulators, and metabolic promoters. Phytochemical analysis shows that alkaloids are dominant, accompanied by flavonoids, saponins, phenolics, and tannins, which contribute to their biological activity. Curry leaf extracts and powder help control pathogenic microflora in livestock digestive tract and reduce the risk of infection. Curry leaves can also suppress inflammation and boost the immune system by increasing antioxidant capacity and reducing oxidative stress. From a sustainability perspective, curry plants are easy to cultivate in tropical regions, can be harvested repeatedly, and have a relatively low environmental impact. However, further research is needed on optimal dosage, long-term safety, molecular mechanisms of action, and the standardization of ingredient quality and regulatory standards for their use as feed additives. In general, the curry leaves are a local phytogenic resource with multifunctional activities, potentially improving livestock health and productivity, and supporting sustainable and environmentally friendly livestock systems.
Analisis konsentrasi dan kemurnian Deoxyribo Nucleic Acid (DNA) dari rumput pakan ternak Syafiradewi Yulianti; Romi Zamhir Islami; Mansyur; Estria Furry Pramudyawardani
Agrivet : Jurnal Ilmu-Ilmu Pertanian dan Peternakan (Journal of Agricultural Sciences and Veteriner) Vol. 12 No. 2 (2024): Desember
Publisher : Fakultas Pertanian Universitas Majalengka

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31949/agrivet.v12i2.10618

Abstract

This research was conducted at the Biotechnology Research and Testing Lab of the Faculty of Animal Husbandry, Padjadjaran University, and the Rice Standard Instrument Testing Center (BBPSI Padi), Subang Regency, West Java starting on September 20 to December 22, 2023. The purpose of this study was to evaluate the effectiveness of the hipure Kit in isolating grass Deoxyribo Nucleic Acid (DNA) by ensuring adequate concentration and cleanliness of samples from contaminants for subsequent molecular applications. The ideal DNA concentration for PCR is 20-50 ng/µL, with an A260/A280 absorbance ratio of about 1.8-2.0 and an A260/A230 ratio above 2.0, indicating the absence of contamination from proteins, salts, carbohydrates, or other organic compounds. The methodology of this study was a descriptive exploration involving descriptive statistical analysis to evaluate the variation in DNA concentration between samples, using spreadsheet software to facilitate data management. The results of the Nanodrop spectrophotometric analysis showed that there variations in DNA concentration between samples, but generally within a range adequate for laboratory applications such as PCR and sequencing. In addition, the HiPure Plant DNA Kit proved effective in producing high-concentration DNA with clear and defined DNA bands in electrophoresis and effectively minimizing contaminants. Further optimization of the extraction protocol, such as adjusting the incubation time or buffer concentration, is recommended to improve DNA concentration and purity. This step will ensure higher DNA quality for downstream applications such as PCR.
KONSENTRASI DAN KEMURNIAN DNA HASIL EKSTRAKSI RUMPUT BENGGALA Khalida Ni'ami Rahmati; Romi Zamhir Islami; Mansyur Mansyur; Deny Saefulhadjar
Agrinimal Jurnal Ilmu Ternak dan Tanaman Vol 14 No 2 (2026): Agrinimal Jurnal Ilmu Ternak dan Tanaman
Publisher : Jurusan Peternakan Fakultas Pertanian Universitas Pattimura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/ajitt.2026.14.2.138-143

Abstract

Rumput benggala (Panicum maximum) merupakan hijauan pakan unggul di wilayah tropis yang memerlukan analisis molekuler untuk mendukung program pemuliaan dan identifikasi genetik. Tahap awal yang krusial dalam analisis tersebut adalah ekstraksi DNA genom berkualitas tinggi yang bebas dari kontaminan. Penelitian ini bertujuan untuk mengevaluasi efektivitas metode ekstraksi DNA menggunakan DNA Mini Kit for Plant GP 100 (Geneaid) dengan modifikasi prosedur pada tiga kultivar rumput benggala (Panicum maximum cv. Purple Guinea, cv. Common, dan cv. Gatton). Modifikasi metode yang diterapkan meliputi stabilisasi sampel melalui penyimpanan pada suhu dingin selama 24 jam sebelum penggerusan dan optimasi pengeringan GD column melalui sentrifugasi pada kecepatan tinggi (13.000 rpm selama 6 menit). Peubah yang diamati adalah konsentrasi dan kemurnian DNA menggunakan spektrofotometer NanoDrop pada panjang gelombang 260 nm dan 280 nm. Hasil penelitian menunjukkan bahwa terdapat variasi efisiensi ekstraksi antarkultivar. Kultivar Purple Guinea menghasilkan rata-rata konsentrasi DNA tertinggi sebesar 92,15 ng/µL dengan indeks kemurnian (A260/A280) 1,87. Kultivar Gatton menghasilkan konsentrasi sebesar 83,78 ng/µL dengan nilai kemurnian tertinggi dan paling konsisten, yaitu 1,90. Sementara itu, kultivar Common menghasilkan konsentrasi terendah sebesar 42,10 ng/µL dengan rata-rata kemurnian 1,82, serta menunjukkan variabilitas kualitas antarsampel yang lebih lebar. Modifikasi metode ekstraksi menggunakan Mini Kit terbukti efektif menghasilkan DNA berkualitas tinggi yang memenuhi standar untuk aplikasi molekuler lanjutan, di mana kultivar Purple Guinea dan Gatton menunjukkan hasil purifikasi terbaik. ABSTRACT Benggala or Guinea grass (Panicum maximum) is a superior forage crop in tropical regions that requires molecular analysis to support breeding and genetic identification programs. A crucial initial step in this analysis is the extraction of high-quality genomic DNA free of contaminants. This study aimed to evaluate the effectiveness of a DNA extraction method using the DNA Mini Kit for Plant GP 100 (Geneaid) with procedural modifications on three guinea grass cultivars (Panicum maximum cv. Purple Guinea, cv. Common, and cv. Gatton). The modifications applied included sample stabilization through cold storage for 24 hours prior to grinding and optimization of GD column drying via high-speed centrifugation (13,000 rpm for 6 minutes). The parameters observed were DNA concentration and purity using a NanoDrop spectrophotometer at wavelengths of 260 nm and 280 nm. The results showed variations in extraction efficiency among cultivars. The Purple Guinea cultivar yielded the highest average DNA concentration at 92.15 ng/µL with a purity index (A260/A280) of 1.87. The Gatton cultivar produced a concentration of 83.78 ng/µL with the highest and most consistent purity value of 1.90. Meanwhile, the Common cultivar produced the lowest concentration at 42.10 ng/µL with an average purity of 1.82, displaying wider quality variability among samples. The modified mini kit extraction method proved effective in generating high-quality DNA that meets the standards for advanced molecular applications, with Purple Guinea and Gatton cultivars demonstrating the best purification results.
Co-Authors Alamsyari . Aminuyati Ana Rochana Anas Anas, Anas Ari Zindhi, Muhammad Amrullah Arif Ragil Saputra Christi, Raden Febrianto Deny Saefulhadjar Dity Asa Priyastomo Estria Furry Pramudyaswari Estria Furry Pramudyawardani Febrianto, Firman Gananto, Adrian Ramadhan Herryawan Kemal Mustafa Heryawan Kemal Mustafa Iin Susilawati Iman Hernaman Khalida Ni'ami Rahmati Lizah Khairani Lizah Khairani Mansyur Mansyur Mansyur . Mansyur Mansyur Mansyur Mansyur Mansyur Mansyur Mansyur Mansyur Mansyur Mansyur Meddy Rachmadi Nena Hilmia Nisa Sakiila Arsadianti Nyimas Popi Indriani Nyimas Popi Indriani Nyimas Popi Indriani Nyimas Popi Indriani Nyimas Popi Indriani nyimas popi indriani Pramudyawardani, Estria Furry Rahmah, Vita Amalia Sidik, Ramdani Suseno Amien Syafiradewi Yulianti Syifa Nurjannah, Syifa Tidi Dhalika Ujang Hidayat Tanuwiria Vita Dwicahyani Warid Ali Qosim Yang Halimah Nur.Azizah