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Journal : Agrointek

PENGARUH KETERSEDIAAN OKSIGEN PADA PRODUKSI EPIGLUKAN OLEH Epicoccum nigrum MENGGUNAKAN MEDIA MOLASES Miftahul Choiron; Jayus Jayus; Sony Suwasono
AGROINTEK Vol 7, No 1 (2013)
Publisher : Agroindustrial Technology, University of Trunojoyo Madura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21107/agrointek.v7i1.2045

Abstract

The compound β-glucan is a polymer of glucose, which is polymerized through betaglycoside bond. The compound β-glucan has anti-tumor benefits, reducing the risk of heartdisease, lowering cholesterol and so forth. The compound β-glucan can be produced by thefungus in ukstraseluler. One of the molds that can produce compounds in the extracellular β-glucan is Epicoccum nigrum, often called epiglucan. The purpose of this study was to determinethe effect of aeration and oxygenation to the production epiglukan by Epicoccum nigrum inmolasses media. This research was conducted using descriptive method with 3 under controlledconditions : anaerobic, aeration and oxygenation in the Stired Continuous Tank Reactor (batchculture). The parameters observed were the biomass (g / L), epiglucan (g/L), reducing sugarcontent (%) and pellet diameter are formed. Biomass and highest epiglikan obtained inoxygenated conditions which amounted to 7.074 g / L and 2.01 g / L. The resulting biomasspellet-shaped with the largest diameter occurs in conditions of oxygenation that is 0.37 mm.residual reducing sugar at the end of fermentation the greatest there is in the anaerobiccondition is 1.529%
ISOLASI DAN IDENTIFIKASI BAKTERI ASAM LAKTAT INDIGENOUS DARI FERMENTASI ALAMI BIJI KAKAO SEBAGAI KANDIDAT AGEN ANTIKAPANG Nurul Isnaini Fitriyana; Sony Suwasono; Joni Kusnadi
AGROINTEK Vol 9, No 1 (2015)
Publisher : Agroindustrial Technology, University of Trunojoyo Madura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21107/agrointek.v9i1.2122

Abstract

This research have focused on isolation and identification of lactic acidbacteria (LAB) with potential antifungal which candidate as biopreservativemicroorganisms. LAB produce bioactive compound with antifungal activity. Thus, of atotal of 69 lactic acid bacteria isolated and 39 isolates further tested its antifungalactivity. Indigenous moulds that will be tested for antifungal activity of LAB wereobtained from unfermented cocoa beans. There were black mould (allegedly Aspergillusniger), green mould (allegedly Aspergillus fumigatus) and grayish white mold (allegedly Mucorspp.). Thus, 18 isolates of LAB that could inhibit all of three types of fungi further identified in aphenotype, 3 isolates of LAB with highest antifungal activity in identification with API 50 CHLmethod and identified as Lactobacillus fermentum, with a different value of ID value, ID99,8% (F1L-6an) criteria : very good identification; ID 99.9% (F1L-6dsr), criteria : verygood identification , and ID 89.8% (F3L-7P) criteria : very good identification to the genus .LAB isolates obtained can be used as starter cultures to improve the quality offermentation to inhibit the growth of mould, mycotoxin production, and fungicideresidues so as to improve quality, safety of cocoa concumption, and increase the priceof Indonesian cocoa bean exports.