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All Journal Buletin Peternakan Jurnal Biologi Udayana Bioma : Berkala Ilmiah Biologi Microbiology Indonesia BIOTROPIA - The Southeast Asian Journal of Tropical Biology AL KAUNIYAH Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati BERITA BIOLOGI JURNAL BIOLOGI INDONESIA ANNALES BOGORIENSES Biosaintifika: Journal of Biology & Biology Education AJARCDE (Asian Journal of Applied Research for Community Development and Empowerment) Annales Bogorienses Berita Biologi
Atit Kanti
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Earth & Planetary Sciences Education Energy Environmental Science Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Veterinary Other

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Journal : BERITA BIOLOGI

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C0-CULTURE OF AMYLOLYTIC FUNGI Aspergillus niger AND OLEAGINOUS YEAST Candida orthopsilosis ON CASSAVA WASTE FOR LIPID ACCUMULATION [Akumulasi lipid oleh kultur campuran kapang Aspergillus niger dan khamir Candida orthopsilosis pada media limbah singkong] Kanti, Atit; Sudiana, I Made
BERITA BIOLOGI Vol 16, No 2 (2017)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (3452.972 KB) | DOI: 10.14203/beritabiologi.v16i2.2207

Abstract

The objective of study was to evaluate the effectiveness of co-culture between amylolytic fungi  Aspergillus niger and lipid accumulating yeast Candida orthopsilosis for lipid accumulation on cassava waste. When grown in 5 % starch medium, all microbes was able to grow, but best growth was observed on Aspergillus niger, other microbes grew slower. Moderate growth was observed  on Saccharomycopsis fibuliger.  The two isolates were then selected for further studies. Aspergillus niger and Saccharomycopsis fibuliger were amylolytic microbes. The amylolytic activities were affected by temperature, pH and nitrogen sources.  Optimum temperature and pH for enzyme production were 30°C and 7.0 respectively. Both yeast extract and sodium nitrate were good nitrogen sources for amylase production. On cassava waste, the highest biomass and total lipid content were obtained by co-culture of Aspergillus niger and lipid accumulating yeast Candida orthopsilosis. Major lipid composition was oleic acids and strearic acids. The ability of co-culture of Aspergillus niger and lipid accumulating yeast Candida orthopsilosis grew and accumulated lipid on cassava waste would suggest that these culture were candidate for biofuel production.
DIVERSITY OF XYLOSE ASSIMILATING YEAST FROM THE ISLAND OF ENGGANO, SUMATERA, INDONESIA [Keragaman Khamir Pengguna Xilose yang Diisolasi dari Pulau Enggano, Sumatera, Indonesia] Kanti, Atit; Sumerta, I Nyoman
BERITA BIOLOGI Vol 15, No 3 (2016)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (3160.618 KB) | DOI: 10.14203/beritabiologi.v15i3.2215

Abstract

Naturally occurring yeasts (wild type) are important genetic resources for many industrial interests. Though biodiversity exploration of Enggano has been done since 1944, but there is no report for yeast diversity from this island. Due to its industrial interest, xylose fermenting yeast is target for many scientists. This present study aims to isolate xylose assimilating yeast from island of Enggano. The samples were collected from varying sources included soil, leaf litter, decay wood, fruit and sediment. Xylose enrichment culture technique was performed to enrich xylose fermenting yeast. While for the isolation of yeast several methods which include dilution, ballistopore falling, direct isolation, membrane filtration were done. Of 200 isolates, 76 strains (38 %) were xylose assimilating yeast which mostly belong to Candida which include C. insectorum, C. tropicalis, C. boidinii, C. pseudolambica, C. yuanshanica, C. silvae, Cyberlindnera saturnus, Williopsis saturnus and Sporobolomyces poonsookiae. One strain Candida sp. that was isolated from soil could be assigned as a candidate of novel species on base on its morphological and biochemical characteristics along with analyses of gene sequence from D1/D2 domain. This study confirms that yeast survey is very important to obtain new genetic resources for industrial interest as well as for taxonomic study.
KEANEKARAGAMAN KHAMIR YANG DIISOLASI DARI SUMBER DAYA ALAM PULAU ENGGANO, BENGKULU DAN POTENSINYA SEBAGAI PENDEGRADASI SELULOSA [Diversity of Yeasts Isolated From Natural Resources of Enggano Island, Bengkulu and Its Cellulolytic Potency] Sumerta, I Nyoman; Kanti, Atit
BERITA BIOLOGI Vol 15, No 3 (2016)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v15i3.2220

Abstract

The present study revealed the occurrence and diversity of yeasts from natural resources of Enggano Island. The yeasts were isolated from soil, sediment, leaf, leaf litter, fruit, and flower. Isolation was performed using direct plating, membrane filtration, ballistospore-falling and enrichment culture using glucose, xylose and xylan as carbon source. After isolating, yeasts were screened for its cellulolytic potency. The occurrence of yeasts in different isolation technique are clearly found. Most of 87 yeasts isolated, abundant in leaf, soil, and sediment sample. The total yeasts isolated were classified into 32 species based on D1/D2 LSU 26S rDNA sequences analysis which genera of Candida were predominant (33%). The predominant yeast species were identified as Candida tropicalis (16,1%), Cyberlindnera saturnus (16,1%), and Rhodosporidium paludigenum (11,5%). Screening on carboxymethyl cellulose medium, there are 43 isolates within 22 yeasts species have cellulolytic activity. Four species of Basidiomycetous have high activity which includes Pseudozyma antartica Y15Eg001; Pseudozyma hubeiensis Y15Eg015; Anthracocystis chrysopogonis Y15Eg072; Cryptococcus laurentii Y15Eg017 and two from Ascomycetous are Sarocladium bactrocephalum Y15Eg226, Y15Eg227. Some of cellulolytic yeast isolates are indicated as new species candidate and required to reconfirm in another conserve regions to ensure its taxonomic position. Various yeasts isolated from Enggano Island with its cellulolytic potency should contribute to scientific information regarding microbial genetic resources of outer islands of Indonesia.
DIVERSITAS DAN PROFIL METABOLIT SEKUNDER JAMUR ENDOFIT YANG DIISOLASI DARI TUMBUHAN GAMBIR (Uncaria gambler) SERTA AKTIVITAS BIOLOGISNYA SEBAGAI ANTIBAKTERI Jamal, Yuliasri; Ilyas, Muhamad; Kanti, Atit; Agusta, Andria
BERITA BIOLOGI Vol 9, No 2 (2008)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (853.072 KB) | DOI: 10.14203/beritabiologi.v9i2.2023

Abstract

Five kinds of endophytic filamentous fungi i.e. Pestalotiopsis sp. GNC, Fusarium sp. GNC-A, Fusahum sp. GNC-B, Pestalotiopsis sp. GUC and Fusarium sp. GUC were isolated from surface sterilized of steems of gambir nasi and gambir udang (Uncaria gambier Roxb.) collected from Rengat, Riau Province,Indonesia. Identification of the endophytic fungi were done through morphologically observations and chemotaxonomically approach. In an antibacterial assays, the ethyl acetate extract of the Pestalotiopsis sp.GNC cultures showed their activity against pathogenic bacteria, Eschericia coli, Staphylococcus aureus and Bacillus sublilis.Beside the above three pathogenic bacteria, the ethyl acetate extract of Fusarium sp. GNC-B culture was also active against
AKTIVITAS CMC-ase KHAMIR Candida sp. YANG DIISOLASI DARI TANAH KEBUN BIOLOGI WAMENA, PAPUA Kanti, Atit; Sudiana, I Made
BERITA BIOLOGI Vol 6, No 5 (2003)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v6i5.1190

Abstract

Cellulolytic Candida sp.was isolated from Wamena, Papua.The strain was able to grow in media with carboxymethyl cellulose as a sole carbon source implying that the isolate produced 1-3 oc endo-gluconase.To study the effect of glucose and osmotic pressure on 0.1% glucose and 0.1% NaCl were augmented to CMC-contained media.Glucose augmentation affects cellulolytic activity of culture,probably due to higher biomass production in media. NaCl addition appear not to affect cellulolytic ability. Profile of pH also varied depend on the cultivation media. Maximum growth rate was achieved when glucose was added which was implying that glucose stimulate cell growth.
DIVERSITY AND ECOLOGICAL PERSPECTIVE OF SOIL YEAST IN GUNUNG HALIMUN NATIONAL PARK*[Khamir Tanah Taman Nasional Gunung Halimun: Keragaman dan Perspektif] Kanti, Atit; Sudiana, I Made
BERITA BIOLOGI Vol 6, No 1 (2002)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (302.168 KB) | DOI: 10.14203/beritabiologi.v6i1.1167

Abstract

Taman Nasional Gunung Halimun merupakan hutan hujan tropika yang berada di pulau Jawa. Penelitian ini ditujukan untuk mengetahui populasi dari khamir tanah pada berbagai ketinggian tempat (600 m, 1000 m, dan 1500 m) di atas permukaan laut, dan selanjutnya mempelajari peran ekologi dari khamir, dilakukan dengan mengisolasi dan menganalisa karakter fisiologi terutama dalam mendekomposisi selulosa dan hidrolisa fosfat. Khamir yang diisolasi dari tanah dan daerah perakaran dimurnikan dan ditumbuhkan pada media yang mengandung carboxymethylcellulose (CMC) sebagai sumber karbon utama, dan juga ditumbuhkan pada media pivoskaya untuk mengetahui kemampuan pelarutan Ca3(PO4)2. Populasi khamir pada daerah yang atas sedikit lebih tinggi .Sebanyak 23 isolat khamir yang termasuk dalam marga Debaryomyces, Pichia, Rhodotorula, dan Candida diisolasi dari tanah.15 isolat mempunyai kemampuan menghidrolisa selulosa dan 9 isolat mampu melarutkan Ca3(PO4)2. Khamir ditemukan di tanah dan daerah perakaran dan memegang peran penting dalam degradasi senyawa organik dan mineralisasi fosfat di dalam tanah.
IDENTIFICATION OF YEASTS ISOLATED FROM GUNUNG HALIMUN NATIONAL PARK*[Identifikasi Khamir pada Taman Nasional Gunung Halimun] Kanti, Atit; Julistiono, Heddy; Sudiana, I Made
BERITA BIOLOGI Vol 6, No 1 (2002)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (596.655 KB) | DOI: 10.14203/bb.v6i1.1177

Abstract

Dua puluh sembilan isolat khamir diisolasi dari tanah Taman Nasional Gunung Halimun. Sumber isolat berasal dari batang pohon lapuk, akar lapuk yang diambil dari Gunung Botol, Cikaniki, dan Cipta Rasa untuk dipelajari aspek taksonominya. Berdasarkan atas karakter morfologi dan fisiologi, isolat-isolat tersebut digolongkan kepada kelompok ascomyceteous, basidiomyceteous dan imperfect khamir. Selanjutnya ketiga golongan tersebut dimasukkan ke dalam sepuluh kelompok (Kelompok 1 sampai X). Dari 29 isolat tersebut, 7 isolat dimasukkan ke dalam kelompok I diindentifikasi sebagai Debaryomyces hansenii, 6 dalam kelompok II sebagai Candida sp, 2 dalam kelompok III sebagai Pichia membranafaciens, 5 isolat dalam kelompok IV sebagai Candida galacta, 1 dalam kelompok V sebagai Candida sake, 4 dalam kelompok VI sebagai Cryptococcus humicolus, 1 dalam kelompok VII sebagai Rhodotorula minuta, 1 dalam kelompok VIII sebagai Candida sp, 1 dalam kelompok Candida sp, dan 1 dalam kelompok X dalam Candida sp. Macam sampel tampaknya tidak berpengaruh kepada keragaman jenis khamir seperti ditunjukkan oleh jenis yang sama diisolasi dari berbagai jenis sampel. Dari banyaknya jenis khamir yang diisolasi menunjukkan bahwa keragaman jenis khamir di Taman Nasional Gunung Halimun tergolong tinggi.
CELLULOLYTIC YEAST ISOLATED FROM SOIL GUNUNG HALIMUN NATIONAL PARK* [Khamir Selulotik yang Diisolasi dari Tanah Taman Nasional Gunung Halimun] Kanti, Atit; Sudiana, I Made
BERITA BIOLOGI Vol 6, No 1 (2002)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (338.421 KB) | DOI: 10.14203/beritabiologi.v6i1.1173

Abstract

Beberapa khamir tanah yang lermasuk dalam marga Debaryomyces dan Candida diisolasi dari tanah Taman Nasional Gunung Halimun.Kemampuan selulotiknya diuji dcngan menumbuhkannya pada media yang mengandung carboxymethyl cellulose (CMC) sebagai sumber karbon utama. Pertumbuhan biomassa dan pH diikuti selama kultivasi. Pengamh penambahan glukosa terhadap aktivitas selulolitik juga dipelajari.Dari 23 isolat yang diuji, 19 termasuk khamir selulolitik, dan 1 isolat yang mempunyai kemampuan tertinggi dipelajari karakterisktik ezymatik sclulasenya. Penambahan glukosa menstimulasi pertumbuhan sel dan menyebabkan kebutuhan sel akan glukosa bertambah, dan sclanjutnya memacu sintesa ensim. Dari isolat-isolat yang diuji, isolat Debaryomyces S-6 mempunyai aktifitas paling tinggi yaitu sebesar 12 UI pada 96 jam inkubasi. Selama waktu kultivasi pH meningkat dari 6,5 menjadi 7. Adanya khamir yang menghidrolisa selulosa mengindikasikan khamir memegang peranan penting dalam transformasi bahan organik dalam tanah.
THE COMMUNITY OF SOIL YEASTS IN GUNUNG HALIMUN NATIONAL PARK Kanti, Atit; Sudiana, I Made; Julistiono, Heddy
BERITA BIOLOGI Vol 5, No 6 (2001)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (409.796 KB) | DOI: 10.14203/beritabiologi.v5i6.1081

Abstract

Fifty-two isolates were isolated from Gunung Halimun National Park on the basis of morphological and some physiological characteristics.Those isolates were belonged to three groups namely, ascomycetous, basidiomycetous and imperfect yeasts.Rhodotolum sp.was only found in Ciptarasa site at 1500 m asl, ascomycetous yeasts was only isolated from deteoretic root in Gunung Botol site, while Candida sp.(small globose shaped cells) was only isolated from soil at 1800 m asl of Gunung Botol site. Type of plant species appeared has no effect on yeasts diversity as shown by similar yeasts diversity was observed at rhizosphere soil of three dominating plant (Schima waallichii, Castanopsis javanica and Altingia excelsa) at Cikaniki study site.
THE PHSYIOLOGICAL CHARACTER OF BACTERIA ISOLATED FROM BANANA’S RHIZOSPHERE FROM MALAKA, EAST NUSA TENGGARA, AND THEIR ROLE ON PLANT GROWTH PROMOTION ON MARGINAL LAND Napitupulu, Toga Pangihotan; Kanti, Atit; Sudiana, I Made
BERITA BIOLOGI Vol 18, No 3 (2019)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v18i3.3748

Abstract

The objective of the study was to isolate microorganism that can produce growth hormone, fix atmospheric nitrogen, and solubilize inorganic phosphate that may be used for enhancing the growth of banana in marginal land.  A total of 25 bacteria associated with banana that belonged to six genera were isolated from namely Acinetobacter, Enterobacter, Pantoea, Klebsiella, Rhizobium, and Cupriavidus genera. Gammaproteobacteria is the dominant class, followed by Betaproteobacteria and Alphaproteobacteria. All isolates were screened for multiple plant growth promoting traits which may play a role in banana growth, namely fixing nitrogen, solubilizing phosphate, and producing Indole Acetic Acid (IAA). Twenty-two isolates were capable to fix nitrogen, 21 isolates can solubilize insoluble phosphate, and 15 isolates produced IAA dependent of L-Tryptophan presence. Despite most of the bacteria isolates exhibited one plant growth-promoting activities, Enterobacter and Klebsiella genera showed three of plant growth promoting bacteria traits. 
Co-Authors Ahmad Thontowi Ahmad Thontowi ANDO KATSUHIKO ANDRIA AGUSTA Anja Meryandini Ariyanti Oetari Arwan Sugiharto Bambang Prasetya Chandra, Nicholas Dwi CHIHARU NAKASHIMA Elsera Br Tarigan, Elsera Br Endang Kusdiyantini Endang Sukara Euis HERMIATI Evana Faradila Ayu, Near Putri Guswenrivo, Ikhsan Habibi, Moh Hafid, Lukman HEDDY JULISTIONO Heddy Julistiono Helbert Helbert I Made Sudiana I Made Sudiana Idris Idris Ikhwani, Azra Zahrah Nadirah Kamal, Andi Saptaji Kartika Dyah Palupi latifah K Darusman Latifah K. Darusman Latupapua, H.J.D. LATUPAPUA, H.J.D. Lestari, Agis Dwi lina marlina, lina Lutfi Nia Kholida Maman Rahmansyah Mia Kusmiati Miftahul Ilmi, Miftahul Muhamad Ilyas Muhamad Ilyas, Muhamad Muhammad Ilyas Mujahidah, Shofia Mujahidah, Shofia N Nurhayati Nampiah Sukarno Napitupulu, Toga P. Napitupulu, Toga Pangihitan Napitupulu, Toga Pangihotan Pandanwangi, Aisyah Amelia Pratama, Aulia Brellian Purbani, Debora Christin Putri, Ade Lia RASTI SARASWATI Raunsai, Marlin Megalestin Rosida Rosida Senlie Octaviana Sri Listiyowati Sudiana, I. Made Sumerta, I Nyoman Sumerta, I Nyoman Sumerta, I Nyoman Sumerta, I Nyoman Suprapedi Suprapedi Urip PERWITASARI Wellyzar Sjamsuridzal WIBOWO MANGUNWARDOYO Yantyati Widyastuti Yopi YULIASRI JAMAL Yuliasri Jamal