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All Journal HAYATI Journal of Biosciences METAMORFOSA Journal of Biological Sciences JBIO: jurnal biosains (the journal of biosciences) Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati Jurnal Pembangunan dan Alam Lestari AGRIVITA, Journal of Agricultural Science Jurnal Penelitian Pendidikan IPA (JPPIPA) JMM (Jurnal Masyarakat Mandiri) Bioscientist : Jurnal Ilmiah Biologi BIOGENESIS Proceeding Biology Education Conference Jurnal Gramaswara: Jurnal Pengabdian kepada Masyarakat JAPI: Jurnal Akses Pengabdian Indonesia
Maharani Pertiwi Koentjoro
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Journal : Proceeding Biology Education Conference

 1 
Produksi Kitosan Secara Enzimatik oleh Bacillus Sampah Perikanan Afifah, Kufah Nur; Koentjoro, Maharani Pertiwi; Prasetyo, Endry Nugroho
Proceeding Biology Education Conference: Biology, Science, Enviromental, and Learning Vol 14, No 1 (2017): Proceeding Biology Education Conference
Publisher : Universitas Sebelas Maret

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Abstract

The ability of Bacillus sp. has been developed by isolation bacteria from fisheries waste to synthesis chitin deaetylase. This microorganism secretes extracellular chitin deacetylase and does not involve cell lysis. Chitin deacetylase has roles as bioconversion which converts chitin to chitosan with deacetylation N-acetylglucosamine residue in chitin. The bioconversion process of chitin into chitosan in this study is occured by Bacillus sp. B3, Bacillus sp. B5, Bacillus sp. B6, and Bacillus sp. B12 through deacetylation enzymatic which is isolated from fisheries waste. The protein component of crude enzyme was measured using Bradford methods with BSA (Bovine Serum Albumin) as standard. The enzyme was purified by ammonium sulfate precipitation. Chitin deacetylase was characterized by measuring isoelectric point, enzyme activity, and protein component. While the fungtional groups of chitosan had characterized by FTIR and measured degree of deacetylation chitosan. The highest enzyme activity was achieved by Bacillus sp. B12 through ammonium sulphate purification at 75-90% fraction is 345.21U/ml with protein content is 0.0512 mg/ml. Chitin deacetylase from fourth Bacillus sp. has an isoelectric point at pH 5. The highest degree of deacetylation chitosan is 67% and the termination of acetyl groups had confirmed by IR spectra.
Penambahan Biochar Berbasis Limbah pada Inceptisol terhadap Budidaya Tembakau (Nicotiana tabacum (L.)) Varietas Kemloko di Temanggung Katon, Damarmoyo Sasongko; Susilo, Buana; Dalimartha, Lengga Nurullah; Wiguna, Eko Chandra; Isdiantoni, Isdiantoni; Koentjoro, Maharani Pertiwi; Prasetiyo, Endry Nugroho
Proceeding Biology Education Conference: Biology, Science, Enviromental, and Learning Vol 14, No 1 (2017): Proceeding Biology Education Conference
Publisher : Universitas Sebelas Maret

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Biochar is a biological charcoal derived from incomplete combustion (pyrolysis) of agricultural residualmaterials that can increase carbon sustainably, retention of water and nutrients in soil. This research aimed todemonstrate the effect of adding biochar on inceptisol for Kemloko tobacco cultivation in Temanggung, CentralJava, Indonesia. The data analysis of this research was using Factorial Random Design with two factors(treatment and dose). The results showed that the addition of biochar + compost increase productivity of 491.6kg/ha comparing to the control with the higher tobacco quality. Chemical analysis of the respected soil showedthat the biochar + compost treatment increase pH level up to 5.46 with the organic matter level and total nitrogenas well increased about 2.66% and 0.180% respectively.
Model Manajemen Terpadu Pertanian Hortikultura Organik pada Lahan Sempit Suryadi, Dede; Megawati, Ayuningtyas; Susilo, Buana; Dalimartha, Lengga Nurullah; Wiguna, Eko Chandra; Isdiantoni, Isdiantoni; Koentjoro, Maharani Pertiwi; Prasetiyo, Endry Nugroho
Proceeding Biology Education Conference: Biology, Science, Enviromental, and Learning Vol 14, No 1 (2017): Proceeding Biology Education Conference
Publisher : Universitas Sebelas Maret

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Abstract

Integrated management of organic farming is an agriculture management system with an effort in developing the organic basis of cropping system to improve soil health, and pest & disease controlling to achieve sustainable productivity. Integrated management model of organic horticulture can be done alternatively on narrow land thereby intercropping and companion of several crops in order to improve yield. For instance, planting of different plants families such as herbs & flower in one area such as kenikir and tagetes to reduce the population of pest and to improve natural enemies population.
Pengaruh Formulasi Penambahan Biochar terhadap Produksi Tanaman Tembakau Varietas K326 Cross Creek Seed USA. di Lahan Kering Kabupaten Bojonegoro Pahlevi, Reza Widhi; Susilo, Buana; Dalimartha, Lengga Nurullah; Wiguna, Eko Chandra; Isdiantoni, Isdiantoni; Koentjoro, Maharani Pertiwi; Prasetiyo, Endry Nugroho
Proceeding Biology Education Conference: Biology, Science, Enviromental, and Learning Vol 14, No 1 (2017): Proceeding Biology Education Conference
Publisher : Universitas Sebelas Maret

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Abstract

Tobacco plant has high value and strongly sensitive to physical and chemical conditions of soil condition. One alternative to increase tobacco production is modify the soil using biochar accompanied with other organic materials such as fertilizer. This research aimed to determine the best formulation on addition of biochar to the yield of tobacco (Nicotiana tabacum) plants var. K326. The study was conducted in Balen Subdistrict, Bojonegoro District, East Java, Indonesia with Randomized Block Design consisting of 5 treatments. Prior aplication the biochar was formulated with compost, OCF (Organic Compound Fertilizer), and agricultural waste. The formulation is intended to allow biochar having adequate nutrient content to increase tobacco yield. Biochar treatment formulated with OCF gave 45,8 % higher of tobacco yield than the control by 1,084 ton ha-1 and 0,497 ton ha-1 respectively.
Co-Authors Adyan Donastin Afifah, Kufah Nur Ahmad Nuril Fuad Al Fatih Al Fatih, Ahmad Nuril Fuad Al zamzami, Ilham Misbakudin Ali Mustafa Sidauruk Alviani, Melinda Nuril Amin, Abd Aziz Andi Kurniawan Anggayasti, Wresti L. Anggraeni, Ike Listya Anthon Efani Arief Rachmansyah Arifin, Nur Hikmah Ashari, Fadhil Muhamad Ayuningtyas Megawati Bambang Semedi Buana Susilo Bunga Hidayati Dalimartha, Lengga Nurullah Dwi Susanti, Yogita Ayu Efani, Anton Eko Chandra Wiguna Endry Nugroho Prasetiyo Endry Nugroho Prasetyo Eti Setiawati, Eti Fitriyah, Ikhrotul Galisong, Ridwan Danuarta Gunawan, Fahdynia Karnira Habibah, Laila Nur Harsuko Riniwati Herni Sudarwati Hidayat, Muhammad Taufiq Ida Ekawati Ina Irawati, Ina Isdianto Isdianto Isdiantoni Isdiantoni Isdiantoni Kartikaningsih, Hartati Katon Sasongko Damarmoyo Katon, Damarmoyo Sasongko Lengga Nurullah Dalimartha Lisa Aulia, Lisa Masruroh, Inayatul Moehammad, Khibar Syiar Mufidah, Elya Muhammad Fathur Rouf Hasan Muhammad Taufiq Hidayat Nurlelih, Euis Elih Pahlevi, Reza Widhi Pramudia, Zulkisam Prasetiyo, Endry Nugroho PUJI LESTARI Rico Hutama Sulistiyo Rini Yulianingsih Salamah, Lutfi Ni’matus Setyo Nugroho Siti Asmaul Mustaniroh Siti Mariyah Ulfa Sri Sudaryanti, Sri SURYADI, DEDE Susilo, Buana Tri Wahyu Hidayat Wati, Henny Diana Wiguna, Eko Chandra Wike, Wike Yanuar, Adi Tiya YOGA DWI JATMIKO Yusuf Efendi