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Pengembangan Protokol Deteksi Staphylococcus aureus Berbasis Molekuler Koentjoro, Maharani Pertiwi; Alviani, Melinda Nuril; Jatmiko, Yoga Dwi; Habibah, Laila Nur; Al Fatih, Ahmad Nuril Fuad; Kartikaningsih, Hartati
Bioscientist : Jurnal Ilmiah Biologi Vol 12, No 1 (2024): June
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v12i1.9494

Staphylococcus aureus becomes a normal flora in humans, especially on the skin and in the nose. However, if it becomes excessive or if there are pathogenic variants, it can cause various health problems. The purpose of the study is to develop a molecular-based detection method for Staphylococcus aureus using the norA primer gene. The norA gene in Staphylococcus aureus is known to play a role in pathogenesis with its antibiotic resistance ability. This type of research is analytical observational with a cross-sectional method. The methods in the study include the isolation and identification of Staphylococcus aureus from healthcare facility equipment. Isolation and identification include bacterial isolation using Blood Agar Plate (BAP) media; isolate purification, Gram staining; biochemical tests using Mannitol Salt Agar (MSA) media, glucose tests, Voges Proskauer (VP) tests, catalase tests, and coagulase tests. Furthermore, S. aureus isolates were tested using a molecular-based method, namely Polymerase Chain Reaction (PCR). This method includes DNA isolation stages, qualitative testing with agarose gel electrophoresis, semi-quantitative testing with image J software, amplification with Real-Time Polymerase Chain Reaction (RT-PCR) using norA gene primers. The Mann-Whitney test results gave a value of p = 0.334 (p> 0.05) indicating the suitability between the culture method and the PCR method with the developed protocol in detecting Staphylococcus aureus. The developed method includes the use of base sequences in the norA gene primer, optimization of annealing and extension temperatures, and the concentration of DNA templates used.

ANTIMICROBIAL ACTIVITY TEST OF MEDICINAL PLANT EXTRACT USING ANTIMICROBIAL DISC AND FILTER PAPER AGAINST Staphylococcus aureus : CLEAR ZONE RESULTS COMPARISON Hidayat, Muhammad Taufiq; Koentjoro, Maharani Pertiwi; Prasetyo, Endry Nugroho
JBIO: jurnal biosains (the journal of biosciences) Vol 10, No 2 (2024): JBIO : JURNAL BIOSAINS (THE JOURNAL OF BIOSCIENCES)
Publisher : Universitas Negeri Medan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24114/jbio.v10i2.43276

Ekstrak tumbuhan mengandung metabolit sekunder berupa flavonoid dan senyawa fenolik yang memiliki aktivitas antibiotik. Uji aktivitas antibiotik dapat dilakukan dengan metode cakram Kirby-Bauer yang aktivitas antibiotiknya ditunjukkan dengan terbentuknya zona bening. Penelitian ini dilakukan dengan menggunakan ekstrak daun Moringa oleifera dan kladode Opuntia cochenillifera yang diekstrak menggunakan etil asetat sebagai pelarut. Uji aktivitas antibiotik terhadap bakteri Staphylococcus aureus dilakukan pada media Mannitol Salt Agar dan Luria Bertani Agar. Cakram Kirby-Bauer yang digunakan adalah Antimicrobial Disc Oxoid® dan Filter Paper. Hasil penelitian ini menunjukkan bahwa penggunaan Antimicrobial disc Oxoid® dan Filter Paper tidak menunjukkan perbedaan secara statistik pada hasil zona bening (t-value = 0,45; p-value = 0,655; dengan α = 95%). Sementara itu, hasil Clear Zone pada Mannitol Salt Agar memberikan hasil yang lebih baik daripada Luria Bertani Agar dan signifikan secara statistik (nilai-t = 2,46; nilai-p = 0,02; dengan α = 95%). Temuan ini menunjukkan bahwa kertas saring dapat menjadi alternatif yang murah untuk uji antibiotik dengan hasil yang tidak berbeda secara signifikan dibandingkan dengan cakram antibiotik komersial. Namun, uji antibiotik terhadap Staphylococcus aureus lebih baik dilakukan pada Mannitol Salt Agar dibandingkan dengan Luria Bertani Agar.

Alteration of the Rhizosphere Bacteria Community Respond Differently to Plant Growth Promoting Rhizobacteria in Peanut Soil’s Poteran Island Ekawati, Ida; Wati, Henny Diana; Koentjoro, Maharani Pertiwi; Sudarwati, Herni; Isdiantoni, Isdiantoni; Prasetyo, Endry Nugroho
AGRIVITA Journal of Agricultural Science Vol 46, No 3 (2024)
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v46i3.3947

Plant growth-promoting rhizobacteria (PGPR) has been detailed to affect soil microbial exercises or community composition. There is a lack of information on the degree to which PGPR as a biofertilizer concurrently influences the action, estimate, and composition of the soil microbial community. This research investigated the impacts of the timing and frequency of PGPR application as biofertilizers on the rhizosphere bacteria community in arable soil. We compared four treatments in an experimental field site, namely: one-time treatment of PGPR at the beginning of planting (P1), twice treatment of PGPR (P2), 15-days of planting (DP), and 30-DP (P3); and without treatment of PGPR (WP). A total rhizosphere bacteria community fingerprint was surveyed using ribosomal intergenic spacer analysis (RISA) using a culture-dependent and culture-independent approach. The rhizosphere bacteria community was surveyed during 80 DP. The unweighted pair-group method with arithmetic mean (UPGMA) clustering showed that the rhizosphere bacteria community in three-frequency applications of PGPR was more abundant than without PGPR application and one or two PGPR applications. This study revealed that the rhizosphere bacteria community was increased in soil with PGPR application, especially in P3, three dosages of PGPR application. Increasing the rhizosphere bacteria community could strongly influence the clay foam soil nutrient.

PENDAMPINGAN PENGUATAN TATA KELOLA PENCEGAHAN, PENANGANAN DAN PEMULIHAN KEKERASAN SEKSUAL DI SATUAN PENDIDIKAN Koentjoro, Maharani Pertiwi; Riniwati, Harsuko; Wike, Wike; Setiawati, Eti; Irawati, Ina; Arifin, Nur Hikmah; Fitriyah, Ikhrotul
JMM (Jurnal Masyarakat Mandiri) Vol 9, No 1 (2025): Februari
Publisher : Universitas Muhammadiyah Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31764/jmm.v9i1.27831

Abstrak: Kekerasan seksual di lingkungan pendidikan, mengalami peningkatan jumlah kasus akibat pertumbuhan penduduk, perkembangan teknologi, dan ketimpangan gender yang belum terselesaikan. Di sisi lain, kesadaran hak asasi manusia terus meningkat. Sebagai upaya pencegahan dan penanganan yang komprehensif, Peraturan Menteri Pendidikan, Kebudayaan, Riset, dan Teknologi Nomor 46 Tahun 2023 menetapkan peraturan mengenai pencegahan dan penanganan kekerasan seksual di satuan pendidikan, termasuk sekolah umum. Tujuan dari kegiatan pengabdian ini untuk membantu meningkatkan pemahaman dan strategi pimpinan satuan pendidikan dalam upaya mencegah kasus kekerasan seksual melalui kolaborasi dengan instansi terkait. Mitra program pengabdian ini adalah guru pengajar di SMP Brawijaya Smart School, Kota Malang sebanyak 30 orang. Sebagai upaya peningkatan kapasitas, metode pengabdian dilakukan melalui diskusi, sosialisasi, dan pelatihan pencegahan serta penanganan kekerasan seksual kepada guru dan tenaga kependidikan. Kegiatan ini menunjukkan hasil positif dengan meningkatnya pemahaman peserta terkait tata kelola kekerasan seksual. Hasil post-test menunjukkan tingkat pemahaman naik sebesar 30%. Program ini diharapkan berkontribusi terhadap penguatan kebijakan pencegahan dan penanganan kekerasan seksual di lingkungan pendidikan di Malang.Abstract: Sexual violence in educational settings has increased in number of cases due to population growth, technological development, and unresolved gender inequality. On the other hand, human rights awareness continues to increase. As a comprehensive prevention and handling effort, the Minister of Education, Culture, Research and Technology Regulation No. 46 of 2023 stipulates regulations regarding the prevention and handling of sexual violence in educational units, including public schools. The purpose of this service activity is to help improve the understanding and strategies of education unit leaders in preventing cases of sexual violence through collaboration with related agencies. The partners of this service programme are thirty teacher at SMP Brawijaya Smart School in Malang City. As a capacity building effort, the service method was carried out through discussions, socialisation, and training on prevention and handling of sexual violence to teachers and education personnel. This activity showed positive results with an increase in participants' understanding of sexual violence governance. The post-test results showed that the level of understanding increased by 30%. This programme is expected to contribute to the strengthening of policies for the prevention and handling of sexual violence in the educational environment in Malang.

The Role of Biofilm on Microplastics as A Vector for Heavy Metals in the Waters of Sendang Biru, Malang Regency Al Fatih, Ahmad Nuril Fuad; Kurniawan, Andi; Koentjoro, Maharani Pertiwi
HAYATI Journal of Biosciences Vol. 32 No. 4 (2025): July 2025
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.32.4.1092-1102

Microplastic contamination in water provides an ideal surface for biofilm formation. It facilitates other pollutants, such as heavy metals, to attach to their surface. This interaction leads to biological and environmental problems. We tested this phenomenon by investigating the presence of microplastics in water, biofilms on surfaces, and heavy metal accumulation during September 2024. It further examines the relationship between microplastic and their contamination in fish. Samples were collected from two stations, namely Kondang Buntung Fish Auction Place (TPI) and the Indonesian Navy Post, water from Sendang Biru, using a purposive sampling method. A total of 20 liters of water and 6 Banyar fish (Rastrelliger kanagurta) specimens were analyzed. In contrast, biofilm morphology and heavy metal content were analyzed using Scanning Electron Microscopy with energy-dispersive X-ray. The result found that fibre-shaped microplastics, with fragments and filaments, dominated water samples. Microplastics 1-5 mm in size show that their colors included black, blue, red, and transparent. Five biofilm morphologies on the microplastic surfaces were identified: platelet, thin film, solid film, sporous, and diatoms. Heavy metals (Pb, Hg, Cd, Al, Mg, Fe) were detected with weight percentages ranging from 0.473 to 2.533%. Microplastics are found in the digestive tracts of fish, predominantly microplastic as filaments followed by fragments and fibers. We found that biofilm-coated microplastics accumulate heavy metals, posing a risk of pollutant transfer to aquatic organisms.

Workshop Klinik Penulisan Jurnal Nasional Penelitian dan Pengabdian Masyarakat sebagai Upaya Pengembangan Kapasitas Sumber Daya Pengajar Koentjoro, Maharani Pertiwi; Yoga Dwi Jatmiko; Bunga Hidayati; Bambang Semedi; Yusuf Efendi; Ahmad Nuril Fuad Al Fatih; Isdiantoni; Ida Ekawati; Endry Nugroho Prasetyo
Jurnal Gramaswara: Jurnal Pengabdian kepada Masyarakat Vol. 3 No. 3 (2023): Jurnal Gramaswara: Jurnal Pengabdian kepada Masyarakat
Publisher : Faculty of Cultural Studies, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.gramaswara.2023.003.03.09

Currently, many teaching resources have not fully understood how to write and submit to a journal. This can be attributed to various factors, such as a lack of training or experience in journal writing, a greater focus on teaching or research activities, or limited opportunities to engage in journal writing communities. Therefore, this workshop aims to enhance the capacity of teaching resources in writing and publishing research and community service in national journals. The implementation method consists of preparation, activity execution, mentoring, and evaluation stages. The results of the activity evaluation show that 80% of participants have improved their abilities to comprehend the structure of scientific journals, writing ethics, effective writing techniques, and 100% of participants are capable of drafting journals according to environmental style guidelines. Measured capacity development of teaching resources enables them to write journals in accordance with the guidelines of each publisher, avoiding rejection. Furthermore, the ability to understand the Open Journal System (OJS) application during submission is essential for teaching resources to publish in nationally accredited journals.

Diseminasi Teknologi Produksi Mini Instalasi Pengolahan Air Limbah (IPAL) Dengan Model Biofilter Sebagai Upaya Pengembangan Produk Unggulan Batik Berbasis Ramah Lingkungan Nurlelih, Euis Elih; Mustaniroh, Siti Asmaul; Yulianingsih, Rini; Mufidah, Elya; Koentjoro, Maharani Pertiwi
JAPI (Jurnal Akses Pengabdian Indonesia) Vol 10, No 1 (2025)
Publisher : Universitas Tribhuwana Tunggadewi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33366/japi.v10i1.6605

Limbah cair dari Industri batik dengan kandungan berbagai zat kimia, seperti pewarna dan bahan pencuci dapat mencemari lingkungan jika tidak diolah dengan baik. Oleh karena itu, sistem pengolahan air limbah yang efektif, ramah lingkungan, dan sesuai dengan kebutuhan usaha kecil menengah (UKM) diperlukan. Kegiatan pengabdian ini bertujuan untuk merancang dan menguji keefektifan Mini Instalasi Pengolahan Air Limbah (Mini IPAL) dengan model biofilter berbasis Teknologi Tepat Guna (TTG) dalam mengolah limbah cair dari proses pembuatan batik agar dapat digunakan kembali atau dibuang tanpa membahayakan lingkungan. Metode yang digunakan meliputi tahap persiapan, tahap pelaksanaan dan tahap evaluasi. Hasil kegiatan berupa Mini IPAL dengan sistem biofilter yang terdiri dari beberapa komponen, seperti bak pencucian, tangki pengendapan, tangki penyaringan yang terdiri dari beberapa lapisan filter (batu besar, pasir gombong kasar, arang, pasir halus, ijuk, dan batu seloidin), serta instalasi perpipaan untuk aliran air limbah. Hasil uji coba menunjukkan bahwa Mini IPAL model biofilter mampu mengurangi kadar polutan secara signifikan, termasuk zat warna dan partikel tersuspensi, dengan efisiensi yang tinggi. Sistem ini juga terbukti ekonomis, mudah dalam perawatan, dan hemat ruang, menjadikannya cocok untuk diterapkan pada UKM di daerah dengan keterbatasan lahan dan sumber daya.

Penggunaan Probiotik Alami Berbahan Rempah pada Tambak Tradisional Blandongan, Kota Pasuruan Kartikaningsih, Hartati; Koentjoro, Maharani Pertiwi; Sudaryanti, Sri; Anggraeni, Ike Listya; Gunawan, Fahdynia Karnira; Ashari, Fadhil Muhamad
Jurnal Gramaswara: Jurnal Pengabdian kepada Masyarakat Vol. 5 No. 2 (2025): Jurnal Gramaswara: Jurnal Pengabdian kepada Masyarakat
Publisher : Faculty of Cultural Studies, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.gramaswara.2025.005.02.11

Traditional farmers in Pasuruan City face challenges in increasing pond productivity due to declining land quality and reliance on natural feed. Milkfish growth is often hampered by lack of feed and increased disease due to environmental changes. Although manufactured feed and probiotics are available, they are too expensive for traditional farmers. Pasuruan City has great potential for the development of milkfish farming, and the Banyu Urip Fish Farmers Group (POKDAKAN) is an experienced community in this field. The use of probiotics made from spices, such as ginger, turmeric, and curcuma, is proposed as an eco-friendly and economical solution as these ingredients are abundant and effective in improving pond water quality and fish disease resistance. The results of probiotic application showed significant improvement, with 85% of farmers reporting up to 30% increase in production, and 90% recording a decrease in fish disease cases. Pond water quality also improved, particularly in ammonia and dissolved oxygen levels. The use of this natural probiotic not only positively impacts the pond environment, but also supports the sustainability of milkfish farming productivity in Pasuruan City.

Pengembangan Protokol Deteksi Staphylococcus aureus Berbasis Molekuler Koentjoro, Maharani Pertiwi; Alviani, Melinda Nuril; Jatmiko, Yoga Dwi; Habibah, Laila Nur; Al Fatih, Ahmad Nuril Fuad; Kartikaningsih, Hartati
Bioscientist : Jurnal Ilmiah Biologi Vol. 12 No. 1 (2024): June
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v12i1.9494

Staphylococcus aureus becomes a normal flora in humans, especially on the skin and in the nose. However, if it becomes excessive or if there are pathogenic variants, it can cause various health problems. The purpose of the study is to develop a molecular-based detection method for Staphylococcus aureus using the norA primer gene. The norA gene in Staphylococcus aureus is known to play a role in pathogenesis with its antibiotic resistance ability. This type of research is analytical observational with a cross-sectional method. The methods in the study include the isolation and identification of Staphylococcus aureus from healthcare facility equipment. Isolation and identification include bacterial isolation using Blood Agar Plate (BAP) media; isolate purification, Gram staining; biochemical tests using Mannitol Salt Agar (MSA) media, glucose tests, Voges Proskauer (VP) tests, catalase tests, and coagulase tests. Furthermore, S. aureus isolates were tested using a molecular-based method, namely Polymerase Chain Reaction (PCR). This method includes DNA isolation stages, qualitative testing with agarose gel electrophoresis, semi-quantitative testing with image J software, amplification with Real-Time Polymerase Chain Reaction (RT-PCR) using norA gene primers. The Mann-Whitney test results gave a value of p = 0.334 (p> 0.05) indicating the suitability between the culture method and the PCR method with the developed protocol in detecting Staphylococcus aureus. The developed method includes the use of base sequences in the norA gene primer, optimization of annealing and extension temperatures, and the concentration of DNA templates used.

ANTIMICROBIAL ACTIVITY TEST OF MEDICINAL PLANT EXTRACT USING ANTIMICROBIAL DISC AND FILTER PAPER AGAINST Staphylococcus aureus : CLEAR ZONE RESULTS COMPARISON Hidayat, Muhammad Taufiq; Koentjoro, Maharani Pertiwi; Prasetyo, Endry Nugroho
JURNAL BIOSAINS Vol. 10 No. 2 (2024): JBIO : JURNAL BIOSAINS (THE JOURNAL OF BIOSCIENCES)
Publisher : Universitas Negeri Medan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24114/jbio.v10i2.43276

Ekstrak tumbuhan mengandung metabolit sekunder berupa flavonoid dan senyawa fenolik yang memiliki aktivitas antibiotik. Uji aktivitas antibiotik dapat dilakukan dengan metode cakram Kirby-Bauer yang aktivitas antibiotiknya ditunjukkan dengan terbentuknya zona bening. Penelitian ini dilakukan dengan menggunakan ekstrak daun Moringa oleifera dan kladode Opuntia cochenillifera yang diekstrak menggunakan etil asetat sebagai pelarut. Uji aktivitas antibiotik terhadap bakteri Staphylococcus aureus dilakukan pada media Mannitol Salt Agar dan Luria Bertani Agar. Cakram Kirby-Bauer yang digunakan adalah Antimicrobial Disc Oxoid® dan Filter Paper. Hasil penelitian ini menunjukkan bahwa penggunaan Antimicrobial disc Oxoid® dan Filter Paper tidak menunjukkan perbedaan secara statistik pada hasil zona bening (t-value = 0,45; p-value = 0,655; dengan Î± = 95%). Sementara itu, hasil Clear Zone pada Mannitol Salt Agar memberikan hasil yang lebih baik daripada Luria Bertani Agar dan signifikan secara statistik (nilai-t = 2,46; nilai-p = 0,02; dengan Î± = 95%). Temuan ini menunjukkan bahwa kertas saring dapat menjadi alternatif yang murah untuk uji antibiotik dengan hasil yang tidak berbeda secara signifikan dibandingkan dengan cakram antibiotik komersial. Namun, uji antibiotik terhadap Staphylococcus aureus lebih baik dilakukan pada Mannitol Salt Agar dibandingkan dengan Luria Bertani Agar.

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