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All Journal Buletin Peternakan Microbiology Indonesia BIOTROPIA - The Southeast Asian Journal of Tropical Biology Indonesian Journal of Biotechnology Elkawnie Livestock and Animal Research Animal Production : Indonesian Journal of Animal Production Indonesian Journal of Chemistry Jurnal Riset Agribisnis dan Peternakan Agroindustrial Journal
Lies Mira Yusiati
Department Of Animal Nutrition And Feed Science, Faculty Of Animal Science, Universitas Gadjah Mada, Yogyakarta, 55281, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Decision Sciences, Operations Research & Management Engineering Environmental Science Immunology & microbiology Industrial & Manufacturing Engineering Materials Science & Nanotechnology Medicine & Pharmacology Social Sciences Veterinary Other

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Pengaruh Suplementasi Probiotik Bakteri Asam Laktat Terhadap Histomorfologi Usus Dan Performan Puyuh Jantan Sapta Chandra Marnadi Hidayat; Sri Harimurti; Lies Mira yusiati
Buletin Peternakan Vol 40, No 2 (2016): BULETIN PETERNAKAN VOL. 40 (2) JUNI 2016
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21059/buletinpeternak.v40i2.9072

Abstract

The present study was conducted to investigate the effects of lactic acid bacteria (LAB) probiotics supplementation on intestinal histomorpholgy and performance of male quail. The LAB probiotics supplement contained of three strains, those are Lactobacillus murinus, Streptococcus thermophilus, and Pediococcus acidilactici. A total of 96 day-old  male quails (DOQ) were randomly divided into four groups of LAB probiotic supplementation reared for 42 days. Oral supplementation of LAB probiotic were 0 (P0), 107(P1), 108 (P2), and 109(P3)CFU/mL/bird/day. Data were analyzed by analysis of variance CRD One-Way Anova continued with Duncan’s New Multiple Range Test (DMRT). The results showed that LAB probiotic treatment affected on the intestinal morphology, body weight gain, and feed conversion of male quail (P <0.05). Height of intestinal villi were 288.45±8.18, 322.72±1.84, 324.82±2.06, and 390.80±13.24 (µm) respectively. Width of intestinal villi were 112.82±2.35, 138.62±2.35, 162.57±17.15 and 130.72±13.45 (µm) respectively. Depth of intestinal crypts were 61.25±1.49, 72.50±6.25, 69.85±4.55, and 69.05±4.57 (µm) respectively. Feed intake were not different, but resulted difference body weight gain, which were 102.85±4.72, 109.20±4.13, 115.07±7.61, and 118.75±2.54 (g), respectively. Feed conversion were 4.30±0.43, 4.26±0.17, 3.91±0.30, and 3.79±0.27, respectively. This study indicated that supplementation of 109 CFU/mL/bird/day of LAB probiotics showed the best result.
Estimasi Sintesis Protein Mikrobia Rumen Menggunakan Ekskresi Derivat Purin dalam Urin dengan Teknik Spot Sampling pada Kambing Bligon dan Kambing Kejobong Dianestu Putra; Lies Mira Yusiati; Ristianto Utomo
Buletin Peternakan Vol 40, No 3 (2016): BULETIN PETERNAKAN VOL. 40 (3) OKTOBER 2016
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21059/buletinpeternak.v40i3.12766

Abstract

This study were aimed to determine the correlation between concentration of purine derivatives (PD) in spot sample with PD total excretion in Bligon and Kejobong goats and determine the appropriate sampling time, in order to predicting microbial protein synthesis in both breeds. Six male Bligon goats and six male Kejobong goats with age range from 8 to 14 months and body weight from 16 to 21 kg were placed in metabolism cages. Peanut straw and water were given to both groups of goats through ad libitum feeding and drinking. The study was done in 14 days for adaptation, 3 days for collection. Sample of feeds, feed residues, and feces were collected and then analyzed to determine dry matter and organic matter content. Spot urine and the total daily urine samples were also collected. Samples collection of spot sampling technique was run by taking the urine periodically with 3 hours intervals at 24 hours. Urine samples were analyzed for the content of creatinine and PD which includes allantoin, uric acid, xanthine, and hypoxanthine. Data were tested for the correlation between concentration of PD spot urine sample with total PD daily excretion. It is known that the concentration of PD and creatinine (µmol/L) for Bligon were 1,418.40 and 202.85 respectively, while for Kejobong were 1,547.40 and 219.68 respectively. Total excretion of PD, allantoin, uric acid, xanthyne and hypoxanthine and creatinine (µmol/W0,75/day) for Bligon were 114.14, 95.86, 17.31, 0.97, and 16.40 respectively, with microbial protein synthesis efficiency was 4.61 g N/kg degraded of organic matter in rumen (DOMR). Total excretion of PD allantoin, uric acid, xanthyne and hypoxanthine and creatinine (µmol/W0,75/day) for Kejobong were 180.18, 158.17, 20.60, 1.40, and 24.87 respectively, with microbial protein synthesis efficiency was 6.90 g N/kg DOMR. Based on this study also known that the best time for spot sampling to determine the total excretion of PD in Bligon was in the range time of 11.00 am to 2.00 pm, with equation Y=1.474X+48.81, while Kejobong goat in the range of 2.00 to 5.00 pm, with equation Y=2.678X+5.692.
IMPROVING PHYSICO-CHEMICAL CHARACTERISTIC AND PALATABILITY OF KING GRASS (Pennisetum hybrid) SILAGE BY INOCULATION OF Lactobacillus plantarum - Saccharomyces cerevisiae CONSORTIA AND ADDITION OF RICE BRAN Ahmad Sofyan; Yantyati Widyastuti; Ristianto Utomo; Lies Mira Yusiati
Buletin Peternakan Vol 41, No 1 (2017): BULETIN PETERNAKAN VOL. 41 (1) FEBRUARI 2017
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21059/buletinpeternak.v41i1.12980

Abstract

This study was conducted to determine effectiveness of inoculants consisted of lactic acid bacteria Lactobacillus plantarum (Lp) and yeast Saccharomyces cerevisiae (Sc) combined with addition of rice bran on the physico-chemical characteristics and palatability of king grass (Pennisetum hybrid) silage. The experiment was arranged on the factorial randomized design (3x3) consisting of the inoculants treatments (control, Lp, Lp+Sc) and the addition level of rice bran (0, 5 and 10%). The measured variables were physico-chemical characteristics i.e. colour, odour, pH, lactic acid, Fleigh points, and palatability of silage. Inoculation of Lp and Lp+Sc improved silage odour and reduced fungal contamination. Silage was treated by Lp+Sc and rice bran (5-10%) showed reduction of pH and an increase of lactic acid and Fleigh points. However, interaction between inoculants and rice bran treatment was not significance. Either inoculation or addition of rice bran tended to enhance the palatability of silage in cattle. It concluded that the addition of inoculants L. plantarum and S. cerevisiae with/without addition of 5-10% rice bran could improve the physico-chemical characteristics of silage and its palatability to ruminant.
Effects of Four Essential Oils on Nutrients Digestibility of In Vitro Ruminal Fermentation Asih Kurniawati; Widodo Widodo; Wayan Tunas Artama; Lies Mira Yusiati
Buletin Peternakan Vol 42, No 2 (2018): BULETIN PETERNAKAN VOL. 42 (2) MAY 2018
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21059/buletinpeternak.v42i2.33522

Abstract

This research was done to study the effect of four essential oils (EOs), as feed additives, on ruminal nutrient digestibility in order to find out candidate of rumen modifier. Those four EOs were destilated from Foeniculum vulgare (Mill), Pinus merkusii (Jungh. & de Vriese), Cymbopogon nardus (L.) Rendle and Melaleuca leucadendra (L.). Their effect on dry matter (DM), organic matter (OM), crude protein (CP) and crude fiber (CF) digestibility were studied using in vitro rumen fermentation technique according to Theodorou method. Data were statistically analysed using analysis of variance factorial 4x5 design. Feed for fermentation substrate consist of Pennisetum purpureum, rice bran and wheat pollard. Essential oil individually was added and mix with substrate to meet the final concentration in fermentation media of 0, 100, 200, 400 and 800 mg/l. P. merkusii, C. nardus and M. leucadendra EOs reduced DM, OM, and CF digestibilities. The decreasing were in line with the increasing of EOs doses. Therefore F. vulgare only reduced CF digestibility. The value of DM, OM and CP digestibilities in fermentation with F. vulgare did not differ from control. CP digestibility differed among EO treatments. In F. vulgare, and P. merkusii, treatments, CP digestibility did not differ from control, whereas overall CP digestibility in C. nardus tended to be higher than control and CP digestibility in M. leucadendra was significantly higher. All EOs addition reduced CF digestibility at all level. CF digestibility in fermentation added 800 mg/l of M. leucadendra EO was slumped to only 14.29% of control. Among four EOs M. leucadendra is the most potent on interrupt rumen feed fermentation whereas P. merkusii and C. nardus in moderate ways and F. vulgare in delicate manner.
Effect of Cashew Nutshell Oil Supplementation as Phenol Source for Protein Protection on In Vitro Nutrient Digestibility Rahma Fitriastuti; Lies Mira Yusiati; Budi Prasetyo Widyobroto; Zaenal Bachruddin; Chusnul Hanim
Buletin Peternakan Vol 43, No 4 (2019): BULETIN PETERNAKAN VOL. 43 (4) NOVEMBER 2019
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21059/buletinpeternak.v43i4.35591

Abstract

The current study was aimed to evaluate the ability of phenol content in cashew nutshell liquid (CNSL) in binding protein and its effect on in vitro nutrient digestibility. This research divided into three steps, there were determination of total phenol and tannins content in CNSL, optimalization binding of CNSL tannin to bovine serum albumin with the determination of protein-percipitable phenolics compound and protein content using Lowry method, after that amount of optimalization supplementation of CNSL was used for measuring on in vitro nutrient digestibility by Tilley and Terry method. The results showed that phenol and tannin content of CNSL were 148.69 g/100 ml and 28.3 g/100 ml. One gram tannin in CNSL could be bound by 65.83 g protein. Optimum tannin were used in this study was 5.85 g tannin or equivalent to 163.91 mg of CNSL. Amount of CNSL were used in various levels, there are 0, 50, 100, 150 dan 200 mg. CNSL supplementation reduced dry matter, organic matter crude protein digestibility in rumen, and didn’t reduce dry matter dan crude protein in vitro post rumen digestibility. Supplementation of CNSL increased feed efficiency on in vitro.
Comparison of Nutrient Digestibility of Bligon and Kejobong Goats Fed by King Grass and Peanut Straw Chusnul Hanim; Lies Mira Yusiati; I Gede Suparta Budisatria; Fandi Widya Rachman
Buletin Peternakan Vol 44, No 1 (2020): BULETIN PETERNAKAN VOL. 44 (1) FEBRUARY 2020
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21059/buletinpeternak.v44i1.36229

Abstract

This experiment aimed to determine the nutrient digestibility of Bligon and Kejobong goats fed by king grass and peanut straw. The sample used was 6 to 8 months of five male Bligon goats, and five male Kejobong goats with the initial body weight of 15 to 20 kg and placed in separated metabolism cages. The diet and water were supplied ad libitum. This experiment was done in 14 days of adaptation period and seven days of the collection period. In the collection period, samples of feed, refusal feed, and feces were collected to get the chemical content includes dry matter (DM), organic matter (OM), ether extract (EE), crude fiber (CF), crude protein (CP), nitrogen-free extract (NFE), and total digestible nutrient (TDN). The data obtained were subjected to a T-test analysis. Results showed that nutrients intake, digested nutrient as well as nutrient digestibility of Bligon and Kejobong goats were not significantly different. However, Kejobong goats had EE and CP intake of 15.42% and 14.02%, digested DM, OM, and NFE of 16.29%, 15.71%, and 13.93% respectively, as well as NFE digestibility 4.37% higher than those Bligon goats (P< 0.05). Therefore, there was no difference in nutrient intake, digested, and digestibility of nutrients in Bligon and Kejobong goats fed by king grass and peanut straw. However, Kejobong goats appeared to have higher EE and CP intake, as well as digested and digestibility of DM, OM, and NFE than Bligon goats. 
Bacteriocin Activity of Lactic Acid Bacteria Isolated from Rumen Fluid of Thin Tail Sheep Okti Widayati; Zaenal Bachruddin; Chusnul Hanim; Lies Mira Yusiati; Nafiatul Umami
Buletin Peternakan Vol 43, No 3 (2019): BULETIN PETERNAKAN VOL. 43 (3) AUGUST 2019
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21059/buletinpeternak.v43i3.36837

Abstract

The objective of this study was to determine the activity and the stability of bacteriocin from lactic acid bacteria (BAL) isolated from rumen fluid of thin-tail sheep under the temperature (80, 100, and 121°C), pH (3, 7, and 10), and the length of storage (for 2 weeks under the temperature -8, 11, and 29°C). Lactic acid bacteria obtained by isolation, selection, and identification of thin-tailed sheep rumen fluid were used for bacteriocin production. The crude bacteriocin was partially purified using 70% ammonium sulfate, then was dialysis for 12 hours. The obtained bacteriocin then tested its inhibitory activity against E.coli (representing Gram-negative) and S. aureus (representing Gram-positive) under temperature (80, 100, and 121°C), pH (3, 7, and 10), and the length of storage (for 2 weeks under the temperature -8, 11, and 29°C). The data of bacteriocin activity based on pH, temperature, and the length of storage were analyzed with factorial, then when there was a significant difference of variable because treatment was continued with Duncan's Multiple Range Test (DMRT) test. The results showed that the bacteriocin activity of the three types of BAL against S.aureus is greater than E.coli. The highest activity was shown in pH 3, while the lowest activity was shown at pH 10 (P<0.01). The highest activity was shown at a heating temperature of 100°C, while the lowest activity was shown at a heating temperature of 80°C (P<0.01). The activity of bacteriocin produced by BAL 0 A, BAL 1 A, and BAL 4 C tended to be stable to the heating temperature of 80, 100, and 121°C but decreased with increasing pH value (pH 3, 7, and 10). The best of bacteriocin activity was found at pH 3 (acid), heating at 100°C, and stored at -8°C for 14 days.
The Effect of Nutmeg Leaves Tannin (Myristica fragrans Houtt) as Protein Protecting Agents on In Vitro Nutrient Digestibility Monica Canadianti; Lies Mira Yusiati; Chusnul Hanim; Budi Prasetyo Widyobroto; Andriyani Astuti
Buletin Peternakan Vol 44, No 1 (2020): BULETIN PETERNAKAN VOL. 44 (1) FEBRUARY 2020
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21059/buletinpeternak.v44i1.47976

Abstract

This experiment was aimed to study the effect of nutmeg leaf tannin addition on in vitro nutrient digestibility. Treatments in this experiment consisted of: P0 (control without tannin), P1 (feed + 2% tannin) and P2 (feed + 4% tannin). Feed for fermentation substrate consisted of Pennisetum purpureum and soybean meal with ratio 60:40. Fermentation was carried out using Tilley and Terry two stages in vitro technique for 48 hours. Variables measured were the digestibility of dry matter, organic matter, and crude protein in the rumen as well as the total digestive tract digestibility based on in vitro technique. The data obtained were analyzed by One Way ANOVA, and followed by the Duncan Multiple Range Test (DMRT). The results showed that rumen dry matter digestibility was lower (P<0.05) in P1 and P2 (59.03±3.24 and 57.19±1.32) compared to P0 (70.77±1.05), but did not show a significant difference (P>0.05) in the total dry matter digestibility of P0, P1, and P2 (74.88±5.28, 67.70±3.21, and 64.83±4.96). Organic matter digestibility in the rumen was also lower (P<0.05) in P1 and P2 (55.55±6.29 and 55.76±6.88) compared to P0 (75.39±0.91), but did not show significant difference (P>0.05) in total organic matter digestibility from P0, P1, and P2 (64.69±6.44, 64.33±6.34, and 61.20±5.11). The digestibility of crude protein in the rumen at P1 and P2 (45.48±5.12 and 38.47±3.44) was also significantly lower (P<0.05) compared to P0 (60.93±9.72), whereas total digestibility did not show any significant difference (P>0.05). Addition of tannin leaf nutmeg 2% optimally reduced rumen dry matter and crude protein digestibility without causing excessive negative impact on results of in vitro digestibility, so it can be used as a protective agent protein feed.
Identification of Glucogenic Amino Acids Content in Gliricidia maculata as an Alternative Energy Source for High-Yielding Periparturient Dairy Cows Sulvia Dwi Astuti SW; Budi Prasetyo Widyobroto; Ali Agus; Lies Mira Yusiati
Buletin Peternakan Vol 44, No 2 (2020): BULETIN PETERNAKAN VOL. 44 (2) MAY 2020
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21059/buletinpeternak.v44i2.54103

Abstract

Gliricidia maculata which has long been used as a forage for animal feed may contain a variety of glucogenic amino acids variants. The objective of this study was to identify the glucogenic amino acids content in Gliricidia maculata as an alternative source of energy for high-yielding periparturient dairy cows. The samples were the edible portion of plants, harvested randomly at the optimal cutting age (80 days), from the feed plant collection garden of The Faculty of Animal Science, Universitas Gadjah Mada. Lyophilization was carried out by drying the samples at 55°C for 3 x 24 hours continouesly in a Sanyo Drying Oven MOV-112. Pulverization was done by the Foss Tecator CyclotecTM 1093 Sample Mill with 300 mesh (1 mm screen). The hydrolysis of amino acid was carried out using HCl solution while amino acid derivatization used O-phtalaldehyde (OPA) solution. Separation, determination, and quantification of amino acid were carried out by an analytical method in gradient elution using the Thermo ScientificTM DionexTM UltiMateTM 3000 UHPLC Systems with Rapid Separation Fluorescence Detector. Result showed there were at least fourteen kinds of amino acids identified from the samples, i.e.: aspartic acid, glutamic acid, serine, histidine, glycine, arginine, alanine, tyrosin, methionine, valine, phenylalanine, isoleucine, leucine and lysine. Gliricidia maculata contains 1349 ppm glucogenic amino acids, 412.7 ppm ketogenic amino acids and 444.7 ppm glucogenic and ketogenic amino acids. Moreover, there were three types of glucogenic amino acids with the highest concentration were serine (288.7 ppm), glutamic acid (245.5 ppm) and phenylalanine (197.1 ppm) respectively. Glucogenic amino acids can be used as an energy source for dairy cows through gluconeogenesis. This study suggests that Gliricidia maculata may use to supply the precursors of energy for high-yielding periparturient dairy cows to prevent ketosis.
Isolation and Identification of Lactic Acid Bacteria on Boiler Chicken Roisu Eny Mudawaroch; Setiyono Setiyono; Lies Mira Yusiati; Edi Suryanto
Elkawnie: Journal of Islamic Science and Technology Vol 6, No 2 (2020)
Publisher : Universitas Islam Negeri Ar-Raniry Banda Aceh

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22373/ekw.v6i2.7015

Abstract

Abstract: The aims of this research were to isolate lactic acid bacteria from broiler chicken. The main material was a chicken boiler aged 30 days. The identification of LAB was carried out based on isolation and identification of morphological, phenotypic, and biochemical characteristics. Thirteen LAB isolates were obtained and 5 selected LAB isolates were identified based on growth levels, pH, and lactic acid levels. Five selected LAB isolates were identified as Lactobacillus genus which has Gram-positive, negative catalase, non-motility, hetero-fermentative, rod shapes,  rod-colony shapes, and cream colony color.Abstrak: Penelitian ini bertujuan untuk mengisolasi bakteri asam laktat dari ayam broiler. Bahan utamanya adalah ayam boiler berumur 30 hari. Identifikasi BAL dilakukan berdasarkan isolasi dan identifikasi karakteristik morfologi, fenotipik, dan biokimia. Tiga belas isolat BAL diperoleh dan 5 isolat BAL terpilih diidentifikasi berdasarkan tingkat pertumbuhan, pH, dan kadar asam laktat. Lima isolat BAL terpilih diidentifikasi sebagai genus Lactobacillus yang memiliki Gram-positif, katalase negatif, non-motilitas, hetero-fermentatif, bentuk batang, bentuk koloni batang, dan warna koloni krem.
Co-Authors Abbas (Abbas) Achmad Dinoto Achmad Dinoto Adhe Humaera Adi Magna Patriadi Nuhriawangsa Agustinah Setyaningrum, Agustinah Ahmad Sofyan Ali Agus Ali Agus Ali Agus Ali Agus Ali Wibowo Ali Wibowo Ali Wibowo Ambar Pratiwiningrum Andriyani Astuti Asih Kurniawati Asih Kurniawati B. Suhartanto Bambang Suhartanto Budi Prasetyo Widyobroto Canadianti, Monica Catur Suci Purwati Chusnul Hanim Diah Tri Widayati Dianestu Putra Edi Suryanto Edi Suryanto Edi Suryanto Edy Suryanto Efka Aris Rimbawanto Ema Damayanti Ema Damayanti Endang Baliarti Erika Kusumawardani Fandi Widya Rachman Firman Nasiu Gardika Windar Prahara H. Hartadi Hari Hartadi Harwanto (Harwanto) I Gede Suparta Budisatria I Gede Suparta Budisatria Ika Sumantri Jamhari (Jamhari) Joko Riyanto Koestantinah Koestantinah, Koestantinah Kunty Novi Gamayanti Kustantinah Kustantinah Lilis Hartati Ludfia Widyasmara mudawaroh, roisu Mudawaroh, Roisu Eny Mugiono (Mugiono) Muhamad Nur Cahyanto Muhamad Nur Cahyanto, Muhamad Nur Muhammad Nur Cahyanto Muhsin Al Anas Nafiatul Umami Nafly C. Tiven Nafly C. Tiven Novita Kurniawati Puput Diah Sitoresmi Rahma Fitriastuti Ristanto Utomo Ristianto Utomo Ristianto Utomo Ristianto Utomo Roisu Eny Mudawaroch Roisu Eny Mudawaroch Roisu Eny Mudawaroch Roisu Eny Mudawaroch Rusman (Rusman) Rusman Rusman Rusman Rusman Sapta Chandra Marnadi Hidayat Setiyono (Setiyono) Setiyono Setiyono Setiyono Setiyono Setiyono Setiyono Setiyono Setiyono Setiyono, Etiyono Soemitro Padmowijoto Soeparno Soeparno Soeparno Soeparno Subur Priyono Sasmito Budhi Suharjono Triatmojo Sujadi (Sujadi) Sulvia Dwi Astuti Supadmo (Supadmo) Suryanto, Edi Syamsul Arifin Tety Hartatik Tri Yuwanta Umar Santoso Umar Santoso Umar Santoso Wahidin Teguh Sasongko Wayan Tunas Artama Wayan Tunas Artama Wibowo, Siti 'Athiya Widayati, Okti Widodo Widodo Widodo Widodo Yantyati Widyastuti Zaenal Bachruddin Zaenal Bachrudin