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THE PREFERENCES AND DISTRIBUTION OF SUGARCANE SCALE INSECT Aulacaspis tegalensis (HEMIPTERA: DIASPIDIDAE) ON DIFFERENT CLONES OF SUGARCANE Pramono, Sudi; Wagiman, Franciscus Xaverius; Trisyono, Yohanes Andi; ., Witjaksono
JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA Vol. 20 No. 1 (2020): MARCH, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/j.hptt.12046-53

Abstract

This research was conducted in the field of Gunung Madu Plantations Co., Gunung Batin, Lampung Tengah, in the period of April 2015–May 2016. The experiment was arranged in a randomized completely block design with six sugarcane clones as treatments (GMP 1, GMP 2, RGM 99.370, RGM 97.8837, GMP 3, and GMP 4) and four blocks, every block was separated by the street. The abundance of sugarcane scale insects was observed in 15-day intervals from the plant ages of 4 to 12 months. The results showed that sugarcane clone affected the abundance of sugarcane scale insects. After a lag phase of six months (fifth observation), the sugarcane scale insects started being found on all six sugarcane clones in the field. The abundance of the scale insects then increased and peaked at 10.5 months. The sugarcane scale insects attacked all six clones of sugarcane. Clone GMP 1 was the most preferred with an average scale abundance at 814.4 individuals/stem while the least preferred clone was GMP 4 with average of 179 individuals/stem.
DETECTION AND TRANSMISSION OF RICE STUNT VIRUS ON CIHERANG AND SITU BAGENDIT VARIETIES Helina, Selvi; Sulandari, Sri; Hartono, Sedyo; Trisyono, Andi
JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA Vol 18, No 2 (2018): SEPTEMBER, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (353.425 KB) | DOI: 10.23960/j.hptt.218169-176

Abstract

Detection and Transmission of  rice stunt virus on Ciherang and Situ Bagendit Varieties. The explosion of brown planthoppers recently has caused reduction of rice production in Indonesia. Brown planthoppers do not only act as pest, but also transmit Rice grassy stunt virus (RGSV) and Rice ragged stunt virus (RRSV). Detection of the existence of the two viruses in rice plants and vector insects is important to be done to ensure that the virus is infected with the vector. The aim of this research is to detect the existence of virus in varieties of Ciherang and Situ Bagendit as a result of transmission in the laboratory and to find out the ability of brown planthoppers to transmit stunt virus to both of the varieties. This research was compiled using Completely Randomized Design (CRD) with 4 treatments, namely healthy rice plants of Ciherang and Situ Bagendit varieties, Ciherang and Situ Bagendit varieties which were infested by brown planthoppers each with 5 repetitions. The parameters observed were incubation period, symptoms, plant height, number of leaves and incidence of disease. The data on plant height, number of leaves and incidence of disease were analyzed using ANOVA and continued with the Least Significant Difference (LSD) test at the level of 5%. The results showed that Ciherang and Situ Bagendit varieties were only positively infected by Rice ragged stunt virus. The results of the rice transmission showed that Ciherang variety had a faster incubation period of 10 DAI while Situ Bagendit was 14 DAI, but the two varieties showed an inhibition of growth in plant height and number of leaves compared to healthy plants with each incidence of 51.3% and 46.3%.
Kendali Ketahanan Genetik Padi terhadap Penyakit Tungro Muliadi, Ahmad; Nasrullah, Nasrullah; Sumardiyono, Y. B.; Trisyono, Y. Andi
Jurnal Penelitian Pertanian Tanaman Pangan Vol 33, No 2 (2014): Agustus 2014
Publisher : Pusat Penelitian dan Pengembangan Tanaman Pangan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (83.134 KB) | DOI: 10.21082/jpptp.v33n2.2014.p87-92

Abstract

Genetic study of resistant to rice tungro disease was carried out in Indonesian Agricultural Biotechnology and Plant Genetic Resources Institute, Bogor. TN1 rice variety was used as susceptible parent and OBSTG02-124 line as resistant parent. Cross combinations between resistant vs susceptible parents obtained 6 crosses. The study materials consisted of 55 plants of susceptible parent (P1) and 35 plants of resistant parents (P2), 70 F1 plants, 70 F1R plants, 100 BC1-1 plants, 100-BC1-2 plants, and 300 F2 plants. The probable maternal effect was identified by comparing resistant to tungro disease of F1 plants vs. the F1 of their reciprocal crosses, using the t-test. Each population was planted in pot containing 5 kg of soil. Plants were inoculated at 7-10 days old with virus tungro isolate Subang, using 4-5 green leafhoppers for 5 hours. Visual symptoms were observed based on the Standard Evaluation System for Rice. ELISA test (non Precoated I-ELISA) was performed at 21 days after inoculation, using polyclonal antibodies RTSV (S) and the combined RTBV and RTSV (BS). The results showed that there was no maternal effect on the inheritance of tungro disease resistance. The resistant to tungro in OBSTG02-124 was controlled by two complementary recessive genes. The moderate heritability indicated that the environment play role in determining the degree of resistant to tungro desease.
Keragaman Virulensi dan Konstruksi Molekuler Virus Tungro pada Padi dari Daerah Endemis Praptana, R. Heru; Sumardiyono, Y. B.; Hartono, Sedyo; Trisyono, Y. Andi; Widiarta, I Nyoman
Jurnal Penelitian Pertanian Tanaman Pangan Vol 33, No 2 (2014): Agustus 2014
Publisher : Pusat Penelitian dan Pengembangan Tanaman Pangan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (191.024 KB) | DOI: 10.21082/jpptp.v33n2.2014.p93-101

Abstract

Tungro is an important disease of rice, constraining to the rice production in Indonesia. Tungro is caused by the infection of two different viruses namely tungro bacilliform virus (RTBV) and tungro spherical virus (RTSV). Both viruses are only transmitted by green leafhoppers, especially Nephotettix virescens in a semipersistent manner. The variation of tungro viruses from different areas had been reported, and there is a specific relationship between resistance variety and tungro virus isolate. It is important therefore, to study the virulences and the genetic diversities of tungro viruses derived from the endemic areas in Indonesia. This study was aimed to identify the virulence and the molecular diversity of tungro viruses from endemic areas in Indonesia. Susceptible variety TN1 was used in the study. Surveys and collection of the infected plants and green leafhoppers were conducted in some tungro endemic areas, including: West Java, Central Java, Yogyakarta, Central Sulawesi, West Sulawesi, South Sulawesi, Bali and West Nusa Tenggara. Artificial virus transmission using test tube method was used in the virulence test. Green leafhoppers caught from the field were used as vector transmitters. The virulence of tungro viruses was determined based on diseases indexes (DI). Results showed that the virulence of tungro viruses varied among region in the endemic areas in Indonesia. The Central Java virus isolate was the most virulence; however, not all isolates from endemic areas in the island of Java were more virulent than those from outside of Java. The presence of RTBV and RTSV was detected in the infected TN1 plants. The existences of molecular diversities of tungro viruses from the endemic areas were observed. The relationship between combination of DNA bands of RTBV and RTSV with the virulence in endemic areas outside of Java was more complex than it was in West Java and Central Java. The molecular diversities of tungro viruses were not correlated with the geographic difference of the endemic areas, nor with the virulences.
Residual Effect of Deltamethrin Insecticide at Rice Plants Against the Parasitism Level of Anagrus Nilaparvatae Parasitoids (Hymenoptera: Mymaridae) Meilin, Araz; Trisyono, Y. Andi; Buchori, Damayanti
Jurnal Penelitian Pertanian Tanaman Pangan Vol 2, No 1 (2018): April 2018
Publisher : Pusat Penelitian dan Pengembangan Tanaman Pangan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (98.414 KB) | DOI: 10.21082/jpptp.v2n1.2018.p9-15

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The impact of deltamethrin on natural enemies can be determined directly through contact and oral applications or indirectly through residues on plants. This research was aimed to determine the indirect impact of deltamethrin on the egg parasitoid of Anagrus nilaparvatae.  The rice plants of Cisadane variety were infested with 10 gravid females of N. lugens. Deltamethrin was applied at two concentrations (12.5 and 6.25 ppm) and at 7, 3, 1 day and 3 hours before the parasitization. Deltamethrin residues on rice plants were decrease the number of Nilaparvata lugens, parasitoid and  parasitization level of A. nilaparvatae. This suggests that deltamethrin residue on rice plants could decrease the role of A. nilaparvatae in controlling N. lugens.
Pewarisan Ketahanan Penyakit Tungro pada Galur Padi OBSTG02-28 Muliadi, Ahmad; Nasrullah, Nasrullah; Sumardiyono, Y. B.; Trisyono, Y. Andi
Jurnal Penelitian Pertanian Tanaman Pangan Vol 30, No 2 (2011): Agustus 2011
Publisher : Pusat Penelitian dan Pengembangan Tanaman Pangan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (96.456 KB) | DOI: 10.21082/jpptp.v30n2.2011.p121-126

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Tungro is the most important viral disease of rice plant, caused by two types of viruses, namely Rice Tungro Bacilliform Virus (RTBV) and Rice Tungro Spherical Virus (RTSV), transmitted by green leafhopper Nephotettix virescens. Planting resistant varieties proved effective in preventing the occurrence of tungro disease explosion, but the availability of tungro virus resistant varieties are still limited. To determine the best breeding strategy, genetic inheritance model for resistance to tungro disease needs to be studied especially the gene action and the number of genes controlling resistance. For this study, variety TN1 was crossed with OBSTG02-28 to produce F1, F1R, BC1-1, BC1-2, and F2. All crosses were inoculated with tungro virus and visual symptoms of the disease were observed, followed with the ELISA test. Scoring of the disease and ELISA test showed that the resistance of OBSTG02-28 was controlled by two complementary genes with a ratio of 9:7. The additive-dominant of gene action model fitted for the inheritance of resistant gene with additive gene action. The narrow sense heritability was considered high; therefore, selection for gene resistance to tungro disease could be conducted in the early generations.
Patogenisitas Virus Tungro pada Varietas Tetua Padi Tahan Tungro R Heru Praptana; Y B Sumardiyono; Y Andi Trisyono
Jurnal Fitopatologi Indonesia Vol 9 No 6 (2013)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (245.731 KB) | DOI: 10.14692/jfi.9.6.186

Abstract

Tungro is one of the major diseases in rice which has become a constraint in increasing rice production in Indonesia. Tungro is caused by infection of two different viruses, i.e. Rice tungro bacilliform virus (RTBV) and Rice tungro spherical virus (RTSV), both of which can only be transmitted by green leafhoppers Nephotettix virescens (Distant) in a semipersistent manner. Since there is an indication of the existance of virulence variation of tungro viruses from different areas and the specific relationship between resistance of varieties and tungro viruses isolates, it is important to study the pathogenicity of tungro viruses from some endemic areas in Indonesia. This study aimed to identify the pathogenicity of tungro viruses from several endemic areas in Indonesia in some resistant varieties. One susceptible variety i.e. TN1 and ten resistant varieties were used in the study. Surveys and collection of infected plants and green leafhoppers were done in tungro endemic areas, namely West Java, Central Java, Yogyakarta, Central Sulawesi, West Sulawesi, South Sulawesi, Bali and West Nusa Tenggara. Artificial transmission using the test tube method was used in the pathogenicity test. Green leafhoppers caught from the field were used as transmitters.. The pathogenicity of tungro viruses were identified by the values of the incidence of tungro and the diseases indexes (DI). The result showed that difference in pathogenicity of tungro viruses in some resistant varieties was observed. Isolates from Central Java, Central Sulawesi and West Nusa Tenggara were able to infect all of resistant varieties and the pathogenicity of Central Java isolate was the highest among the other isolates.
Komposisi dan Konsentrasi Senyawa dalam Minyak Atsiri Jeruk Manis dan Jeruk Besar terhadap Perkembangan Tungau Panonychus citri McGregor Mizu Istianto; K Untung; - Mulyadi; Y A Trisyono; T Yuwono
Jurnal Hortikultura Vol 16, No 1 (2006): Maret 2006
Publisher : Indonesian Center for Horticulture Research and Development

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jhort.v16n1.2006.p%p

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Tungau Panonychus citri (Acarina: Tetranychidae)  adalah salah satu hama penting yang menyerang tanaman jeruk di Indonesia. Salah satu kunci sukses untuk mengendalikan populasi P. citri adalah memahami interaksi hama ini dengan inangnya. Namun demikian, informasi dalam bidang ini masih sangat terbatas. Tujuan dari penelitian ini adalah untuk mengetahui perbedaan pengaruh minyak atsiri dari kulit buah jeruk manis dan jeruk besar terhadap perkembangan dan kemampuan reproduksi  tungau P. citri serta mengidentifikasi faktor penyebabnya.  Penelitian dilakukan di Laboratorium  Loka Penelitian Jeruk, Tlekung-Batu, Malang dan Universitas Gadjah Mada Yogyakarta mulai bulan Februari sampai Juli 2003. Perlakuannya adalah beberapa konsentrasi minyak atsiri, yaitu 10, 20, 40, 80 ppm, serta parafin dan kontrol.  Tiap perlakuan diulang 15 kali menggunakan  rancangan acak lengkap. Hasil penelitian menunjukkan bahwa minyak atsiri jeruk manis Pacitan dan jeruk besar Nambangan mampu menghambat perkembangan  dan menurunkan kemampuan reproduksi tungau P. citri pada kondisi laboratorium. Perkembangan yang terhambat  terlihat pada umur pradewasa yang menjadi lebih lama  dan umur dewasa lebih pendek dibanding perlakuan parafin dan kontrol.  Penurunan kemampuan reproduksi terlihat pada lebih sedikitnya telur yang diletakkan dan menetas dibanding perlakuan parafin dan kontrol.  Pengaruh negatif ini disebabkan oleh adanya senyawa limonen yang merupakan senyawa dominan dalam minyak atsiri jeruk.  Minyak atsiri jeruk besar Nambangan  mempunyai pengaruh negatif yang lebih kuat terhadap perkembangan dan kemampuan reproduksi P. citri dibanding minyak atsiri dari jeruk manis Pacitan.  Perbedaan pengaruh tersebut karena perbedaan kandungan senyawa linalool, di mana pada minyak atsiri jeruk besar kandungannya lebih sedikit dibanding pada minyak atsiri jeruk manis.  Linalool berperan mengurangi pengaruh negatif yang disebabkan oleh senyawa limonen. Hasil ini mengungkapkan ada peluang lain dalam mengendalikan tungau P. citri, yaitu memanfaatkan senyawa atsiri yang dihasilkan oleh tanaman itu sendiri dengan komposisi tertentu.  Panonychus citri is one of the most economically  important citrus pests in Indonesia. One of the key success for controlling the population of the pest is understanding the relationship between this mite and its host.  However, information in this area is not well understood. The objectives of this research were to evaluate the influences of essential oil  extracted from sweet orange and pummelo fruit peels on the development and reproductive capacity of P. citri and to understand the mechanism responsible for the different effects that will be useful to develop management program.  The research was conducted in the laboratory from February to July 2003 in Tlekung-Batu, Malang and Gadjah Mada University Yogyakarta. The treatments were 10, 20, 40, 80 ppm of essential oil, parafin and control. Each treatment was replicated 15 times and arranged  in a completely randomized design. The results showed that the essential oil  extracted from Pacitan sweet orange and Nambangan pummello fruit peels could inhibit the development and reduced  the reproductive capacity of P. citri. The essential oils prolonged the life cycle and reduced the fecundity of P. citri.  These negative effects were caused by limonene, a dominant compound in the citrus essential oil.  The negative effects of essential oil extracted from Nambangan pummelo were found to be more pronounced than that from Pacitan sweet orange. Concentration of linalool was found to be responsible for the differences, and it worked oppositely with limonene by reducing the negative effects of limonene on P. citri. Essential oil of Pacitan sweet orange contained more linalool than pummelo. This result gives an alternative technology to control P. citri by using volatile compounds produced by the plant itself with certain composition.
POPULASI Bemisia tabaci (GENNADIUS) PADA PERTANAMAN CABAI DI YOGYAKARTA: STUDI KASUS PADA DAERAH ENDEMIK DAN NON ENDEMIK PENYAKIT KERITING KUNING CABAI Ahmad Taufiq Arminudin; Arman Wijonarko; Y. Andi Trisyono
Jurnal Agroteknologi Vol 1, No 1 (2010): Agustus 2010
Publisher : Universitas Islam Negeri Sultan Syarif Kasim Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24014/ja.v1i1.45

Abstract

This research was aimed to study actual population of Bemisia tabaci, the vector of Pepper Yellow Leaf Curl Virus, in endemic and non endemic areas of Pepper Yellow Leaf Curl Disease (PYLCD) at Yogyakarta through population of its adult and immature. Field observation was taken at Sleman District as endemic areas and Bantul district as non endemic areas. We took sticky yellow trap and modified vacuum to collect actual population of adult whiteflies, whereas the immature were observed by direct counting and collecting the infested leaf of chili. The next activity was identifying and analyzing male and female the population by binocular microscope at Entomology Laboratory Faculty of Agriculture Gadjah Mada University Yogyakarta. The results showed that adult population of B. tabaci in endemic areas was higher than non endemic areas of PYLCD on chili vegetative phase, whereas the immature population was lower. This condition indicated that migration pattern has been occurred on population B. tabaci.
Hubungan Kepadatan dan Biting Behaviour Nyamuk Anopheles farauti Dengan Kasus Malaria di Ekosistem Pantai dan Rawa (Kabupaten Biak Numfor dan Asmat) Hanna S.I. Kawulur; Hidayat Soesilohadi; Suwarno Hadisusanto; Y. Andi Trisyono
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 19, No 1 (2014): February 2014
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v19i1.452

Abstract

AbstractPopulation density and bitting behaviour of insect vectors are several of the factors that influence the number of cases of malaria. This study aims to determine the relationship between population density and bitting behaviour Anopheles farauti which is a vector of malaria in coastal ecosystems (Biak Numfor Regency) and swamp ecosystems (Asmat Regency) with malaria cases. The method used is human landing collection conducted at 18:00 to 6:00 a.m. inside and outside the house. The results showed that the population density of An. farauti in coastal ecosystems is relatively lower than the swamp ecosystems. Man bitting rate in coastal ecosystems is 4 and 4.66, at 95.52 and 42.38 in swamp ecosystem. An. farauti on two ecosystems research are eksofilik. Population density and bitting behaviour An. farauti in coastal ecosystems and swamp ecosystems are not positively correlated with the number of malaria cases.Keywords: population density, biting behavior, An. farauti, Biak Numfor, AsmatAbstrakKepadatan populasi dan aktivitas menggigit serangga vektor merupakan salah satu faktor yang mempengaruhi jumlah kasus malaria. Penelitian ini bertujuan menentukan hubungan kepadatan populasi dan aktivitas menggigit Anopheles farauti yang merupakan vektor malaria di ekosistem pantai (Kabupaten Biak Numfor) dan ekosistem rawa (Kabupaten Asmat) dengan kasus malaria. Metode yang digunakan adalah human landing collection yang dilakukan pada pukul 18.0006.00 di dalam dan di luar rumah. Hasil penelitian menunjukkan bahwa padat populasi An. farauti di ekosistem pantai relatif lebih rendah dibandingkan ekosistem rawa. Man bitting rate di ekosistem pantai adalah 4 dan 4,66 di ekosistem rawa 95,52 dan 42,38. An. farauti pada dua ekosistem penelitian bersifat eksofilik. Kepadatan populasi dan aktivitas mencari darah An. farauti di ekosistem pantai dan ekosistem rawa tidak berkorelasi positif dengan jumlah kasus malaria.Kata kunci: kepadatan populasi, aktivitas menggigit, An. farauti, Biak Numfor, Asmat
Co-Authors - Mulyadi - Mulyadi - Muryati - Witjaksono Abdul Aziz Agustina Dwi Wijayanti Ahmad Taufiq Arminudin Ananto Triyogo Araz Meilin Araz Meilin Araz Meilin Arman Wijonarko Arman Wijonarko Arman Wijonarko Aryuwandari, Valentina E. F. Aye Kyawt Kyawt Ei Aziz Purwantoro Azzahra, Zulfa Bambang Sumiarto Bambang Sumiarto Budi Setiadi Daryono DAMAYANTI BUCHORI De Faveri, Stefano Didik Indradewa Donny Satria, R. Gagak Dwi Wijayanti Dwi Wijayanti Dyah Rini Indriyanti Eddy Mahrub Edhi Martono Edhi Martono Edhi Martono Edhi Martono George Andrew Charles Beattie George Andrew Charles Beattie Hanna Kawulur, Hanna Hanna S.I. Kawulur Hanna S.I. Kawulur, Hanna S.I. Harahap, Nafsiyah Agustina Harjanto, Sriyanto Harwanto (Harwanto) Helina, Selvi Hendrayanti, Hadvina Nur Hidayat Soesilohadi I. Indriyati Wibisono Ignatius Julijantono Ika Puji Rahmawati K Untung Kasumbogo Untung Kurnia Pratiwi Melhanah Melhanah Mizu Istianto Mizu lstianto Mofit Eko Poerwanto Muliadi, Ahmad Muliadi, Ahmad Muryati Muryati Nasrullah, Nasrullah Nasrullah, Nasrullah Nemay Anggadewi Ndaong, Nemay Anggadewi Nur Khasanah Palupi Jatuasri Paul Holford Praptana, R. Heru R Heru Praptana R. Gagak Donny Satria R. Gagak Donny Satria R.C. Hidayat Soesilohadi Rachmawati, Jeti Sari, Riya Fatma SEDYO HARTONO Serafina T.S. Rahayu Setyaningrum, Ary V. Siwi Indarti Somowiyarjo, Susamto Sri Suharni Siwi SRI SULANDARI St. Margino Subandi Subejo Subiadi Subiadi Subiadi Subiadi Suciati Hadi Wuryaningsih Sudi Pramono . Sumardi . Sumardi Sumardi Sumardiyono, Y. B. Sumardiyono, Y. B. Sumardiyono, Y. B. Suprihanto, Suprihanto Supriyadi Supriyadi Suputa Suputa Suputa Suputa Suryani, Julia Nanda Susamto Somowiyarjo SUWARNO HADISUSANTO T Yuwono Tanjung, Heru Rezki TRIS HARIS RAMADHAN Triwibowo Yuwono Vijaysegaran, Shanmugam Wagiman, Franciscus Xaverius Wahyono - Wahyono Wahyono Wahyu Listyaningrum Widiarta, I Nyoman Witjaksono . Witjaksono Witjaksono Witjaksono Witjaksono Witjaksono Witjaksono Y B Sumardiyono Yosefus F. da-Lopez Yuantomoputro, Arzaq P. Yuni Ratna